==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=8-MAR-2013 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER TRANSFERASE 10-AUG-12 4GKA . COMPND 2 MOLECULE: PURINE NUCLEOSIDE PHOSPHORYLASE; . SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS; . AUTHOR A.M.HAAPALAINEN,M.C.HO,J.J.SUAREZ,S.C.ALMO,V.L.SCHRAMM . 1608 12 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 61928.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 1112 69.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 218 13.6 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 132 8.2 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 6 0.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 125 7.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 199 12.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 352 21.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 31 1.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 6 6 0 0 0 18 1 0 8 5 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 1 0 6 6 6 0 6 0 0 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 6 6 0 6 0 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 5 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 3 A N 0 0 82 0, 0.0 144,-0.0 0, 0.0 146,-0.0 0.000 360.0 360.0 360.0 118.1 18.7 6.3 13.7 2 4 A G + 0 0 55 2,-0.0 2,-0.4 0, 0.0 91,-0.0 0.484 360.0 83.7 -77.7 -6.3 21.7 4.3 12.7 3 5 A Y - 0 0 27 3,-0.0 2,-0.1 4,-0.0 90,-0.0 -0.867 66.4-149.6-101.5 135.0 21.7 5.8 9.1 4 6 A T > - 0 0 75 -2,-0.4 4,-1.0 1,-0.1 3,-0.1 -0.392 35.0-103.6 -82.6 170.5 23.2 9.1 8.2 5 7 A Y H > S+ 0 0 49 1,-0.2 4,-2.1 2,-0.2 3,-0.2 0.845 122.7 58.2 -60.1 -33.5 21.9 11.3 5.4 6 8 A E H > S+ 0 0 135 1,-0.2 4,-3.5 2,-0.2 5,-0.2 0.859 96.5 59.0 -68.8 -37.7 24.8 10.1 3.3 7 9 A D H > S+ 0 0 43 2,-0.2 4,-1.7 1,-0.2 -1,-0.2 0.858 109.6 45.3 -56.0 -40.8 23.9 6.4 3.6 8 10 A Y H X S+ 0 0 3 -4,-1.0 4,-2.3 2,-0.2 -2,-0.2 0.936 114.8 47.0 -65.8 -49.8 20.5 7.3 2.0 9 11 A K H X S+ 0 0 96 -4,-2.1 4,-3.3 1,-0.2 5,-0.2 0.917 111.3 51.3 -62.7 -42.7 22.1 9.4 -0.7 10 12 A N H X S+ 0 0 91 -4,-3.5 4,-2.4 1,-0.2 -1,-0.2 0.917 110.4 48.1 -61.4 -45.7 24.7 6.8 -1.5 11 13 A T H X S+ 0 0 0 -4,-1.7 4,-1.5 -5,-0.2 -1,-0.2 0.903 114.7 47.4 -59.3 -41.5 22.1 4.1 -1.8 12 14 A A H X S+ 0 0 3 -4,-2.3 4,-3.3 2,-0.2 3,-0.2 0.947 111.0 49.8 -65.6 -47.5 20.1 6.4 -4.1 13 15 A E H X S+ 0 0 98 -4,-3.3 4,-2.2 1,-0.3 -2,-0.2 0.898 107.6 55.4 -59.8 -41.6 23.1 7.4 -6.3 14 16 A Y H < S+ 0 0 49 -4,-2.4 -1,-0.3 -5,-0.2 -2,-0.2 0.889 113.1 41.9 -55.9 -42.4 24.0 3.7 -6.7 15 17 A L H >X S+ 0 0 0 -4,-1.5 3,-2.0 -3,-0.2 4,-1.1 0.943 112.1 53.0 -72.1 -46.9 20.5 3.1 -8.0 16 18 A L H 3< S+ 0 0 44 -4,-3.3 -2,-0.2 1,-0.3 -1,-0.2 0.804 112.6 46.6 -58.7 -30.9 20.4 6.3 -10.2 17 19 A S T 3< S+ 0 0 85 -4,-2.2 -1,-0.3 -5,-0.2 -2,-0.2 0.195 112.8 48.9 -97.5 10.6 23.7 5.2 -11.8 18 20 A H T <4 S+ 0 0 95 -3,-2.0 2,-0.3 1,-0.3 -2,-0.2 0.365 115.1 35.9-126.4 -2.0 22.7 1.6 -12.4 19 21 A T < - 0 0 6 -4,-1.1 -1,-0.3 -3,-0.1 25,-0.0 -0.985 55.6-154.2-142.8 151.8 19.3 2.1 -14.0 20 22 A K + 0 0 195 -2,-0.3 -1,-0.1 -3,-0.1 -4,-0.1 0.509 66.2 112.1 -96.2 -11.1 17.9 4.8 -16.4 21 23 A H - 0 0 33 1,-0.1 -2,-0.1 47,-0.0 49,-0.0 -0.373 50.9-165.2 -57.1 140.4 14.4 4.1 -15.1 22 24 A R - 0 0 122 47,-0.1 2,-0.1 83,-0.0 53,-0.1 -0.877 10.6-154.4-133.3 97.4 13.1 7.2 -13.2 23 25 A P - 0 0 8 0, 0.0 53,-0.3 0, 0.0 3,-0.1 -0.456 21.8-172.3 -79.5 146.4 10.1 6.3 -11.1 24 26 A Q S S+ 0 0 16 51,-2.7 83,-3.6 1,-0.2 84,-1.5 0.655 78.2 43.7 -93.8 -29.2 7.4 8.7 -10.0 25 27 A V E -ab 76 108A 0 50,-1.4 52,-2.1 81,-0.2 2,-0.4 -0.956 65.1-156.5-126.9 132.4 5.6 6.2 -7.8 26 28 A A E -ab 77 109A 0 82,-2.9 84,-3.2 -2,-0.4 2,-0.4 -0.878 10.7-164.9-103.6 143.9 6.9 3.6 -5.4 27 29 A I E -ab 78 110A 0 50,-2.5 52,-2.4 -2,-0.4 2,-0.6 -0.983 8.1-155.0-130.1 123.3 4.7 0.5 -4.5 28 30 A I E -ab 79 111A 1 82,-2.8 84,-1.4 -2,-0.4 2,-0.5 -0.844 14.4-150.7 -97.3 116.1 5.4 -1.8 -1.5 29 31 A C E -a 80 0A 0 50,-2.7 52,-0.9 -2,-0.6 3,-0.1 -0.783 13.2-155.5 -91.0 123.5 4.0 -5.3 -2.1 30 32 A G > - 0 0 2 -2,-0.5 3,-2.1 1,-0.2 4,-0.2 -0.054 44.7 -40.3 -82.3-171.9 2.8 -7.2 1.0 31 33 A S T 3 S+ 0 0 49 1,-0.3 -1,-0.2 2,-0.1 31,-0.1 -0.237 131.0 6.5 -51.0 130.1 2.5 -10.9 1.7 32 34 A G T 3 S+ 0 0 57 29,-0.4 -1,-0.3 -3,-0.1 3,-0.2 0.466 110.0 92.5 72.9 4.1 1.0 -12.8 -1.3 33 35 A L X + 0 0 4 -3,-2.1 3,-1.4 1,-0.1 -2,-0.1 0.231 44.5 104.0-115.6 12.3 1.2 -9.6 -3.5 34 36 A G G > + 0 0 15 27,-0.3 3,-2.0 1,-0.2 4,-0.3 0.761 64.1 79.3 -63.6 -25.9 4.6 -10.1 -5.1 35 37 A G G > S+ 0 0 22 1,-0.3 3,-1.4 -3,-0.2 4,-0.4 0.643 70.1 83.9 -58.1 -13.8 2.9 -11.2 -8.3 36 38 A L G X S+ 0 0 4 -3,-1.4 3,-1.0 1,-0.3 -1,-0.3 0.737 77.3 67.8 -63.2 -22.8 2.2 -7.5 -9.1 37 39 A T G X S+ 0 0 19 -3,-2.0 3,-1.8 1,-0.2 -1,-0.3 0.796 84.9 72.5 -65.3 -27.5 5.8 -7.3 -10.6 38 40 A D G < S+ 0 0 133 -3,-1.4 -1,-0.2 -4,-0.3 -2,-0.2 0.793 91.5 57.4 -53.3 -32.4 4.5 -9.6 -13.4 39 41 A K G < S+ 0 0 68 -3,-1.0 33,-0.4 -4,-0.4 -1,-0.3 0.481 83.2 105.9 -84.9 -4.7 2.6 -6.7 -14.8 40 42 A L < - 0 0 15 -3,-1.8 2,-0.4 -4,-0.1 3,-0.3 -0.483 57.9-149.6 -72.9 145.7 5.6 -4.4 -15.2 41 43 A T E S+C 70 0A 78 29,-1.9 29,-2.1 1,-0.2 -2,-0.1 -0.938 78.4 17.2-114.4 144.7 7.0 -3.8 -18.7 42 44 A Q E S- 0 0 142 -2,-0.4 2,-0.2 1,-0.2 -1,-0.2 0.912 90.8-168.9 62.5 46.5 10.7 -3.1 -19.4 43 45 A A E - 0 0 58 -3,-0.3 2,-0.4 26,-0.1 26,-0.2 -0.463 19.5-170.5 -75.6 132.3 11.5 -4.5 -15.9 44 46 A Q E -C 68 0A 66 24,-2.1 24,-2.5 -2,-0.2 2,-0.4 -0.998 14.4-153.8-118.9 129.9 14.9 -4.1 -14.4 45 47 A I E -C 67 0A 93 -2,-0.4 2,-0.4 22,-0.2 22,-0.2 -0.872 14.6-175.7-108.7 129.7 15.5 -6.1 -11.2 46 48 A F E -C 66 0A 6 20,-2.8 20,-2.8 -2,-0.4 2,-0.3 -0.986 23.9-134.7-113.4 132.5 17.9 -5.5 -8.3 47 49 A D E > -C 65 0A 69 -2,-0.4 3,-1.0 18,-0.2 4,-0.4 -0.630 21.2-124.5 -74.0 143.8 18.2 -8.0 -5.5 48 50 A Y G > S+ 0 0 1 16,-2.6 3,-1.4 -2,-0.3 6,-0.3 0.863 110.4 64.5 -56.5 -33.5 18.1 -6.3 -2.1 49 51 A S G 3 S+ 0 0 71 15,-0.4 -1,-0.2 1,-0.3 4,-0.1 0.813 100.9 49.4 -61.7 -31.2 21.4 -8.1 -1.3 50 52 A E G < S+ 0 0 95 -3,-1.0 -1,-0.3 2,-0.1 -2,-0.2 0.478 92.9 90.7 -87.1 -2.8 23.2 -6.1 -4.1 51 53 A I S X S- 0 0 6 -3,-1.4 3,-1.5 -4,-0.4 -40,-0.1 -0.827 85.3-112.5 -97.9 126.8 21.9 -2.7 -3.0 52 54 A P T 3 S+ 0 0 47 0, 0.0 -2,-0.1 0, 0.0 -38,-0.0 -0.289 94.3 1.9 -54.6 138.7 24.1 -0.8 -0.6 53 55 A N T 3 S+ 0 0 62 1,-0.2 -4,-0.1 -4,-0.1 43,-0.1 0.442 94.7 142.0 68.4 5.5 22.6 -0.3 3.0 54 56 A F < - 0 0 20 -3,-1.5 -1,-0.2 -6,-0.3 2,-0.1 -0.585 45.3-133.4 -87.2 136.7 19.5 -2.3 2.2 55 57 A P - 0 0 9 0, 0.0 2,-0.3 0, 0.0 -4,-0.1 -0.263 24.6-112.8 -73.0 174.6 17.9 -4.7 4.7 56 58 A R - 0 0 135 -2,-0.1 2,-0.8 -7,-0.0 8,-0.1 -0.845 11.8-123.5-118.1 143.0 16.8 -8.2 3.9 57 59 A S + 0 0 24 -2,-0.3 26,-0.1 1,-0.2 5,-0.0 -0.761 28.7 177.1 -81.9 114.8 13.4 -9.9 3.7 58 60 A T + 0 0 82 -2,-0.8 -1,-0.2 2,-0.1 25,-0.1 0.513 51.4 93.0 -93.5 -5.9 13.6 -12.8 6.0 59 61 A V S > S- 0 0 12 23,-0.3 3,-2.1 3,-0.1 23,-0.1 -0.728 81.2-119.1 -94.2 124.9 9.9 -13.9 5.6 60 62 A P T 3 S+ 0 0 119 0, 0.0 3,-0.1 0, 0.0 -2,-0.1 -0.346 102.1 39.0 -58.1 146.9 9.2 -16.6 3.0 61 63 A G T 3 S+ 0 0 66 1,-0.1 2,-0.7 -30,-0.1 -29,-0.4 0.170 92.4 96.2 93.3 -16.7 6.9 -15.2 0.3 62 64 A H < - 0 0 11 -3,-2.1 19,-0.2 1,-0.1 -1,-0.1 -0.906 64.4-161.9-105.6 99.5 8.6 -11.8 0.5 63 65 A A - 0 0 55 -2,-0.7 18,-0.2 1,-0.1 -1,-0.1 0.771 20.8-130.7 -63.3 -37.1 11.0 -12.2 -2.5 64 66 A G + 0 0 11 16,-2.5 -16,-2.6 1,-0.3 2,-0.4 0.860 56.7 132.4 88.9 40.2 13.5 -9.4 -1.7 65 67 A R E -CD 47 80A 97 15,-1.8 15,-3.2 -18,-0.2 2,-0.6 -0.980 48.1-148.9-127.2 133.7 13.7 -7.7 -5.1 66 68 A L E -CD 46 79A 0 -20,-2.8 -20,-2.8 -2,-0.4 2,-0.6 -0.913 26.4-168.3 -93.0 117.2 13.5 -4.0 -6.0 67 69 A V E -CD 45 78A 4 11,-2.7 11,-2.5 -2,-0.6 2,-0.2 -0.941 7.3-165.3-120.7 113.5 11.9 -3.9 -9.5 68 70 A F E +CD 44 77A 2 -24,-2.5 -24,-2.1 -2,-0.6 2,-0.3 -0.640 36.1 94.6 -87.7 150.4 11.8 -0.8 -11.7 69 71 A G E - D 0 76A 1 7,-2.2 7,-2.9 -2,-0.2 2,-0.4 -0.993 68.1 -53.8 164.7-160.4 9.5 -0.5 -14.8 70 72 A F E -CD 41 75A 59 -29,-2.1 -29,-1.9 -2,-0.3 2,-0.5 -0.941 33.3-168.3-116.6 132.7 6.2 0.7 -16.2 71 73 A L E > S- D 0 74A 0 3,-3.0 3,-2.6 -2,-0.4 -31,-0.1 -0.981 83.1 -20.1-121.8 116.6 2.8 -0.2 -14.8 72 74 A N T 3 S- 0 0 94 -2,-0.5 -1,-0.2 -33,-0.4 3,-0.1 0.947 130.8 -52.4 46.8 50.1 -0.1 0.7 -17.0 73 75 A G T 3 S+ 0 0 68 1,-0.2 2,-0.4 -3,-0.1 -1,-0.3 0.359 113.2 123.5 72.4 -4.2 2.4 3.2 -18.7 74 76 A R E < - D 0 71A 27 -3,-2.6 -3,-3.0 1,-0.0 2,-0.5 -0.763 64.7-122.9 -95.8 135.0 3.4 4.7 -15.3 75 77 A A E + D 0 70A 21 -2,-0.4 -51,-2.7 -5,-0.2 -50,-1.4 -0.613 51.5 147.1 -75.4 116.7 7.0 4.8 -14.3 76 78 A C E -aD 25 69A 1 -7,-2.9 -7,-2.2 -2,-0.5 2,-0.4 -0.895 42.0-135.0-146.1 174.5 7.4 3.1 -10.9 77 79 A V E -aD 26 68A 0 -52,-2.1 -50,-2.5 -2,-0.3 2,-0.5 -0.999 21.0-153.8-135.7 136.4 9.5 1.0 -8.6 78 80 A M E -aD 27 67A 0 -11,-2.5 -11,-2.7 -2,-0.4 2,-0.9 -0.932 14.5-144.7-118.8 128.0 8.2 -1.9 -6.6 79 81 A M E -aD 28 66A 3 -52,-2.4 -50,-2.7 -2,-0.5 2,-1.1 -0.841 17.0-159.7 -78.6 108.1 9.4 -3.4 -3.4 80 82 A Q E S+aD 29 65A 17 -15,-3.2 -16,-2.5 -2,-0.9 -15,-1.8 -0.789 75.5 4.8 -89.3 91.9 8.7 -7.1 -4.0 81 83 A G S S- 0 0 4 -2,-1.1 2,-0.2 -52,-0.9 -52,-0.2 -0.051 78.9-148.1 99.5 147.8 8.8 -8.0 -0.3 82 84 A R - 0 0 2 -2,-0.1 -23,-0.3 -54,-0.1 2,-0.3 -0.763 16.1 -99.9-138.2-178.3 9.1 -5.7 2.7 83 85 A F - 0 0 0 11,-0.4 2,-0.4 -2,-0.2 -26,-0.1 -0.785 27.9-157.4-112.2 152.0 10.5 -5.6 6.2 84 86 A H > > - 0 0 9 -2,-0.3 3,-1.7 1,-0.1 5,-0.9 -0.982 26.7-129.2-134.3 144.3 8.7 -6.0 9.6 85 87 A M G > 5S+ 0 0 1 132,-0.4 3,-2.3 -2,-0.4 605,-0.1 0.878 108.0 63.4 -49.9 -45.8 9.4 -5.1 13.1 86 88 A Y G 3 5S+ 0 0 8 108,-0.5 604,-2.8 131,-0.3 -1,-0.3 0.695 89.2 68.6 -59.5 -19.3 8.8 -8.7 14.2 87 89 A E G < 5S- 0 0 10 -3,-1.7 -1,-0.3 602,-0.2 -2,-0.2 0.696 126.0 -98.0 -70.9 -17.5 11.8 -9.8 12.2 88 90 A G T < 5S+ 0 0 36 -3,-2.3 -3,-0.2 -4,-0.3 -2,-0.2 0.333 79.7 140.0 115.4 0.1 13.9 -8.0 14.7 89 91 A Y < - 0 0 38 -5,-0.9 -1,-0.3 -4,-0.1 -2,-0.1 -0.570 51.0-129.3 -78.7 129.2 14.4 -4.8 12.6 90 92 A P >> - 0 0 41 0, 0.0 4,-1.9 0, 0.0 3,-0.9 -0.341 33.2-107.5 -65.0 159.9 14.3 -1.4 14.2 91 93 A L H 3> S+ 0 0 5 1,-0.2 4,-2.1 2,-0.2 5,-0.1 0.747 115.9 65.3 -69.4 -23.1 11.9 0.9 12.4 92 94 A Y H 34 S+ 0 0 71 2,-0.2 -1,-0.2 1,-0.2 4,-0.1 0.833 109.3 41.6 -68.9 -27.4 14.6 3.0 10.8 93 95 A K H X4 S+ 0 0 75 -3,-0.9 3,-1.5 2,-0.2 -2,-0.2 0.913 109.5 57.2 -76.5 -46.3 15.6 -0.1 8.9 94 96 A V H 3< S+ 0 0 6 -4,-1.9 -11,-0.4 1,-0.3 -2,-0.2 0.897 118.5 34.1 -50.8 -40.0 12.0 -1.1 8.2 95 97 A T T >X S+ 0 0 4 -4,-2.1 3,-1.3 1,-0.2 4,-0.9 0.331 83.3 109.7-101.5 5.6 11.5 2.3 6.5 96 98 A F H X> S+ 0 0 0 -3,-1.5 4,-1.4 1,-0.3 3,-0.9 0.904 76.0 56.3 -49.2 -47.5 14.9 2.9 5.0 97 99 A P H 3> S+ 0 0 0 0, 0.0 4,-3.0 0, 0.0 -1,-0.3 0.800 95.0 66.9 -57.6 -29.6 13.6 2.4 1.4 98 100 A V H <> S+ 0 0 1 -3,-1.3 4,-1.5 1,-0.2 -2,-0.2 0.886 103.8 43.0 -65.0 -38.0 11.0 5.1 1.8 99 101 A R H S+ 0 0 0 -4,-3.0 5,-2.0 1,-0.3 4,-0.6 0.873 106.6 55.0 -48.3 -43.5 12.6 6.5 -3.0 102 104 A H H ><5S+ 0 0 54 -4,-1.5 3,-1.5 1,-0.2 -1,-0.3 0.939 108.9 46.5 -56.0 -49.7 12.2 10.2 -2.0 103 105 A L H 3<5S+ 0 0 41 -4,-2.2 -1,-0.2 -3,-0.3 -2,-0.2 0.754 103.9 62.6 -67.3 -26.1 15.8 10.9 -3.2 104 106 A L H 3<5S- 0 0 12 -4,-2.1 -1,-0.3 -5,-0.2 -2,-0.2 0.673 124.6-104.5 -67.0 -17.4 15.1 8.9 -6.4 105 107 A G T <<5 + 0 0 53 -3,-1.5 -3,-0.2 -4,-0.6 -2,-0.1 0.427 68.0 153.2 103.0 3.4 12.5 11.6 -7.2 106 108 A V < - 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