==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=22-MAR-2013 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER SIGNALING PROTEIN 16-AUG-12 4GN1 . COMPND 2 MOLECULE: RAS-ASSOCIATED AND PLECKSTRIN HOMOLOGY DOMAINS-CO . SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS; . AUTHOR Y.C.E.CHANG,J.WU . 981 6 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 48058.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 617 62.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 9 0.9 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 219 22.3 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 8 0.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 6 0.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 4 0.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 66 6.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 99 10.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 206 21.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 12 1.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 6 6 0 0 0 0 4 0 8 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 3 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 4 10 4 13 4 1 7 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 268 A V 0 0 110 0, 0.0 22,-0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 116.5 -3.5 -20.6 22.3 2 269 A K - 0 0 150 19,-0.1 20,-2.0 1,-0.1 2,-0.3 -0.193 360.0-144.2 -62.1 138.6 -0.1 -22.3 22.7 3 270 A K E -A 21 0A 119 18,-0.2 2,-0.3 19,-0.1 18,-0.2 -0.704 14.5-171.6 -97.1 149.4 1.7 -22.2 26.0 4 271 A L E -A 20 0A 72 16,-3.2 16,-3.7 -2,-0.3 2,-0.7 -0.957 17.7-144.0-145.4 130.4 5.5 -21.9 26.4 5 272 A V E -A 19 0A 92 -2,-0.3 2,-0.2 14,-0.3 14,-0.2 -0.816 25.6-171.1 -88.0 114.9 7.8 -22.1 29.5 6 273 A I E -A 18 0A 11 12,-2.6 12,-1.8 -2,-0.7 2,-0.5 -0.693 19.0-131.1 -99.7 158.5 10.7 -19.7 29.2 7 274 A R E -A 17 0A 141 -2,-0.2 74,-0.5 10,-0.2 2,-0.4 -0.968 24.7-166.7-111.0 130.0 13.7 -19.4 31.4 8 275 A V E -Ab 16 81A 0 8,-3.0 8,-2.5 -2,-0.5 2,-0.5 -0.960 15.8-137.4-122.4 129.9 14.6 -15.9 32.7 9 276 A H E -Ab 15 82A 19 72,-2.8 74,-2.6 -2,-0.4 6,-0.3 -0.750 25.7-144.9 -84.6 125.0 17.8 -14.7 34.4 10 277 A M > - 0 0 15 4,-3.3 3,-1.6 -2,-0.5 74,-0.1 -0.203 30.9 -91.3 -85.9-179.7 17.1 -12.5 37.3 11 278 A S T 3 S+ 0 0 75 1,-0.3 73,-0.1 72,-0.2 -1,-0.1 0.745 124.3 51.9 -68.1 -25.2 19.1 -9.5 38.5 12 279 A D T 3 S- 0 0 109 2,-0.1 -1,-0.3 1,-0.0 3,-0.1 -0.048 120.9-101.1-102.6 28.0 21.2 -11.6 40.8 13 280 A D S < S+ 0 0 94 -3,-1.6 -2,-0.1 1,-0.2 2,-0.0 0.294 87.3 122.3 73.1 -11.7 22.3 -14.2 38.2 14 281 A S - 0 0 48 -5,-0.1 -4,-3.3 1,-0.0 2,-0.3 -0.256 52.5-139.4 -69.0 170.0 19.7 -16.7 39.4 15 282 A S E -A 9 0A 50 -6,-0.3 2,-0.3 -3,-0.1 -6,-0.2 -0.948 16.6-170.5-138.0 154.3 17.1 -18.0 37.0 16 283 A K E -A 8 0A 106 -8,-2.5 -8,-3.0 -2,-0.3 2,-0.3 -0.992 16.1-135.5-141.8 146.5 13.4 -18.8 37.0 17 284 A T E +A 7 0A 100 -2,-0.3 2,-0.3 -10,-0.2 -10,-0.2 -0.710 26.3 171.1 -95.9 158.8 11.0 -20.5 34.7 18 285 A M E -A 6 0A 24 -12,-1.8 -12,-2.6 -2,-0.3 2,-0.4 -0.944 32.0-112.5-162.8 146.8 7.5 -19.1 33.8 19 286 A M E +A 5 0A 106 -2,-0.3 2,-0.3 -14,-0.2 -14,-0.3 -0.654 33.2 175.7 -84.3 133.4 4.8 -19.9 31.4 20 287 A V E -A 4 0A 3 -16,-3.7 -16,-3.2 -2,-0.4 2,-0.3 -0.837 18.1-138.2-122.0 164.1 4.0 -17.4 28.6 21 288 A D E > -A 3 0A 29 -2,-0.3 3,-1.7 -18,-0.2 46,-0.4 -0.861 27.5-104.5-123.5 168.9 1.5 -17.8 25.8 22 289 A E T 3 S+ 0 0 69 -20,-2.0 46,-0.2 -2,-0.3 -19,-0.1 0.574 115.0 54.4 -71.5 -8.3 1.6 -16.9 22.1 23 290 A R T 3 S+ 0 0 185 -21,-0.2 -1,-0.3 44,-0.1 2,-0.1 0.588 83.9 111.8 -94.8 -10.8 -0.6 -13.9 22.4 24 291 A Q < - 0 0 24 -3,-1.7 42,-1.4 41,-0.1 43,-0.4 -0.374 55.2-144.4 -79.9 141.3 1.3 -12.1 25.2 25 292 A T B > -L 65 0B 37 40,-0.2 4,-1.8 -2,-0.1 40,-0.2 -0.530 33.4-107.4 -82.9 164.8 3.2 -8.8 24.8 26 293 A V H > S+ 0 0 0 38,-2.0 4,-3.6 35,-0.4 5,-0.2 0.881 122.0 60.6 -57.9 -40.1 6.5 -8.3 26.8 27 294 A R H > S+ 0 0 7 35,-2.6 4,-2.5 1,-0.2 -1,-0.2 0.941 105.3 45.9 -51.8 -53.7 4.4 -5.9 28.9 28 295 A Q H > S+ 0 0 91 34,-0.3 4,-1.3 2,-0.2 -1,-0.2 0.860 113.7 48.9 -59.1 -39.3 2.1 -8.7 30.0 29 296 A V H X S+ 0 0 0 -4,-1.8 4,-2.5 2,-0.2 -2,-0.2 0.915 111.3 49.6 -67.7 -45.4 5.1 -11.0 30.6 30 297 A L H X S+ 0 0 0 -4,-3.6 4,-3.0 2,-0.2 -2,-0.2 0.883 104.9 57.7 -59.4 -40.9 6.8 -8.4 32.7 31 298 A D H X S+ 0 0 38 -4,-2.5 4,-2.2 -5,-0.2 -1,-0.2 0.904 110.2 45.9 -57.6 -37.4 3.7 -7.8 34.7 32 299 A N H X S+ 0 0 58 -4,-1.3 4,-2.8 2,-0.2 5,-0.3 0.930 109.3 52.7 -69.8 -46.3 3.8 -11.5 35.6 33 300 A L H X S+ 0 0 8 -4,-2.5 4,-1.5 2,-0.2 6,-0.3 0.921 110.5 49.7 -54.0 -44.4 7.6 -11.4 36.4 34 301 A M H X>S+ 0 0 8 -4,-3.0 4,-1.6 1,-0.2 5,-0.8 0.957 112.9 46.2 -59.0 -48.9 6.8 -8.4 38.8 35 302 A D H <5S+ 0 0 105 -4,-2.2 -2,-0.2 1,-0.2 -1,-0.2 0.891 114.5 45.7 -59.6 -46.7 4.0 -10.3 40.5 36 303 A K H <5S+ 0 0 165 -4,-2.8 -1,-0.2 1,-0.2 -2,-0.2 0.761 117.9 42.0 -75.7 -19.8 5.8 -13.6 41.0 37 304 A S H <5S- 0 0 29 -4,-1.5 -1,-0.2 -3,-0.3 -2,-0.2 0.672 93.0-136.9-100.6 -18.9 9.0 -12.1 42.2 38 305 A H T <5 + 0 0 163 -4,-1.6 -3,-0.1 -5,-0.2 -4,-0.1 0.760 56.3 140.5 64.9 32.6 7.6 -9.4 44.6 39 306 A C < - 0 0 46 -5,-0.8 -4,-0.1 -6,-0.3 -5,-0.1 0.653 62.6-113.6 -77.8 -17.7 10.1 -6.9 43.3 40 307 A G - 0 0 41 -6,-0.4 -1,-0.1 2,-0.0 -5,-0.0 0.264 38.0 -67.7 90.3 142.2 7.6 -4.1 43.3 41 308 A Y + 0 0 85 -3,-0.1 2,-0.2 3,-0.0 3,-0.0 -0.151 58.2 147.7 -61.3 152.3 6.2 -2.2 40.4 42 309 A S > - 0 0 28 207,-0.1 3,-1.3 3,-0.1 208,-0.1 -0.724 51.2-130.6 176.8 139.1 8.1 0.2 38.1 43 310 A L T 3 S+ 0 0 26 1,-0.2 207,-0.1 -2,-0.2 206,-0.1 0.661 105.2 68.6 -65.5 -17.7 8.1 1.2 34.5 44 311 A D T 3 + 0 0 21 45,-0.1 42,-3.2 2,-0.1 -1,-0.2 0.541 67.5 109.7 -83.4 -12.9 11.9 0.5 34.5 45 312 A W E < -C 85 0A 22 -3,-1.3 2,-0.3 40,-0.3 40,-0.2 -0.509 54.4-175.8 -62.0 130.8 11.6 -3.3 34.8 46 313 A S E -C 84 0A 0 38,-2.2 38,-1.6 -2,-0.2 2,-0.5 -0.903 32.9-134.2-135.7 156.0 12.7 -4.6 31.5 47 314 A L E -C 83 0A 0 13,-0.3 13,-2.2 -2,-0.3 2,-0.4 -0.974 30.5-171.6-113.1 126.4 13.1 -7.8 29.6 48 315 A V E -CD 82 59A 0 34,-2.8 34,-2.9 -2,-0.5 2,-0.4 -0.935 9.6-156.8-121.1 135.9 16.4 -8.0 27.8 49 316 A E E -CD 81 58A 8 9,-2.5 9,-2.0 -2,-0.4 2,-0.4 -0.920 6.1-151.3-109.2 141.0 17.6 -10.6 25.3 50 317 A T E - D 0 57A 27 30,-2.1 2,-0.7 -2,-0.4 30,-0.4 -0.881 9.8-144.1 -99.9 144.2 21.2 -11.4 24.6 51 318 A V E >>> - D 0 56A 0 5,-2.3 5,-1.3 -2,-0.4 3,-1.0 -0.948 19.7-164.3-102.5 105.9 22.3 -12.6 21.1 52 319 A S G >45S+ 0 0 52 -2,-0.7 3,-0.6 1,-0.3 -1,-0.1 0.831 79.1 54.1 -69.6 -40.3 24.9 -15.0 22.2 53 320 A E G 345S+ 0 0 97 156,-0.4 -1,-0.3 1,-0.2 158,-0.1 0.653 119.6 36.2 -60.2 -19.8 26.8 -15.6 19.0 54 321 A L G <45S- 0 0 0 -3,-1.0 -1,-0.2 2,-0.1 -2,-0.2 0.290 101.0-128.1-126.6 2.2 27.3 -11.8 18.6 55 322 A Q T <<5 + 0 0 76 -3,-0.6 132,-2.3 -4,-0.5 2,-0.3 0.884 67.6 112.2 50.0 53.7 27.8 -10.7 22.2 56 323 A M E < -DE 51 186A 3 -5,-1.3 -5,-2.3 130,-0.2 2,-0.3 -0.893 44.9-157.8-143.7 175.2 25.1 -7.9 22.1 57 324 A E E -DE 50 185A 23 128,-2.7 128,-3.2 -2,-0.3 2,-0.3 -0.999 4.4-161.9-156.0 153.4 21.8 -7.2 23.8 58 325 A R E -D 49 0A 6 -9,-2.0 -9,-2.5 -2,-0.3 2,-0.6 -0.995 23.9-125.5-138.1 150.8 18.5 -5.3 23.5 59 326 A I E -D 48 0A 17 -2,-0.3 -11,-0.2 -11,-0.2 3,-0.0 -0.834 38.0-127.1 -84.6 120.0 15.7 -4.3 25.7 60 327 A F - 0 0 0 -13,-2.2 -13,-0.3 -2,-0.6 2,-0.3 -0.363 21.7-112.9 -58.8 147.1 12.5 -5.5 24.0 61 328 A E > - 0 0 6 1,-0.1 3,-2.0 -15,-0.1 -35,-0.4 -0.674 27.2-120.7 -79.3 146.3 9.8 -2.9 23.5 62 329 A D T 3 S+ 0 0 2 -2,-0.3 -35,-2.6 1,-0.3 -34,-0.3 0.671 107.5 60.5 -67.3 -19.0 6.8 -3.7 25.7 63 330 A H T 3 S+ 0 0 59 -37,-0.2 -1,-0.3 -38,-0.2 2,-0.2 0.421 82.3 112.1 -83.2 -2.1 4.4 -4.0 22.9 64 331 A E S < S- 0 0 18 -3,-2.0 -38,-2.0 1,-0.1 2,-0.6 -0.539 74.5-118.4 -85.5 141.3 6.3 -6.8 21.3 65 332 A N B > -L 25 0B 38 -40,-0.2 4,-1.3 -2,-0.2 -40,-0.2 -0.637 25.0-158.1 -71.4 116.1 5.0 -10.3 21.0 66 333 A L H > S+ 0 0 2 -42,-1.4 4,-2.3 -2,-0.6 3,-0.5 0.961 87.0 50.7 -62.4 -51.4 7.5 -12.3 23.0 67 334 A V H > S+ 0 0 23 -43,-0.4 4,-1.8 -46,-0.4 -1,-0.2 0.906 108.1 54.0 -57.9 -45.3 6.9 -15.7 21.5 68 335 A E H 4 S+ 0 0 119 1,-0.2 4,-0.4 2,-0.2 -1,-0.3 0.838 109.6 47.5 -58.1 -36.1 7.2 -14.3 18.0 69 336 A N H >< S+ 0 0 10 -4,-1.3 3,-1.2 -3,-0.5 -1,-0.2 0.893 107.9 55.5 -72.1 -35.6 10.6 -12.9 18.8 70 337 A L H >< S+ 0 0 24 -4,-2.3 3,-2.3 1,-0.3 -2,-0.2 0.809 94.1 67.4 -68.9 -32.6 11.7 -16.1 20.4 71 338 A L T 3< S+ 0 0 136 -4,-1.8 -1,-0.3 1,-0.3 -2,-0.2 0.735 89.6 67.9 -59.6 -23.4 10.9 -18.1 17.2 72 339 A N T < S+ 0 0 37 -3,-1.2 -1,-0.3 -4,-0.4 -2,-0.2 0.617 82.1 98.3 -65.5 -13.4 13.8 -16.1 15.6 73 340 A W S < S- 0 0 56 -3,-2.3 2,-0.1 -4,-0.2 136,-0.0 -0.396 83.5-107.6 -75.0 153.1 16.2 -18.0 17.8 74 341 A T > - 0 0 67 1,-0.1 3,-2.0 135,-0.1 5,-0.1 -0.423 30.6-108.7 -72.5 158.3 18.1 -21.0 16.5 75 342 A R T 3 S+ 0 0 262 1,-0.3 -1,-0.1 -2,-0.1 -2,-0.1 0.905 123.6 37.5 -46.9 -45.6 17.2 -24.4 17.7 76 343 A D T 3 S+ 0 0 133 2,-0.0 -1,-0.3 0, 0.0 -2,-0.1 -0.058 88.7 157.5-100.1 27.5 20.5 -24.5 19.7 77 344 A S < - 0 0 31 -3,-2.0 -4,-0.1 1,-0.1 -26,-0.0 -0.249 33.5-165.0 -59.2 137.9 20.4 -20.8 20.7 78 345 A Q + 0 0 145 2,-0.1 -1,-0.1 -28,-0.0 -27,-0.0 0.097 46.3 135.1-106.3 16.2 22.4 -19.8 23.8 79 346 A N - 0 0 15 -5,-0.1 2,-0.3 -28,-0.1 -28,-0.2 -0.327 43.7-146.5 -57.0 146.0 20.6 -16.5 23.8 80 347 A K - 0 0 95 -30,-0.4 -30,-2.1 -72,-0.0 2,-0.4 -0.880 9.1-140.9-119.3 152.1 19.4 -15.5 27.3 81 348 A L E -bC 8 49A 5 -74,-0.5 -72,-2.8 -2,-0.3 2,-0.4 -0.906 23.5-164.9-103.4 139.4 16.4 -13.6 28.6 82 349 A I E -bC 9 48A 32 -34,-2.9 -34,-2.8 -2,-0.4 2,-0.7 -0.985 14.7-157.5-134.2 127.4 17.1 -11.2 31.4 83 350 A F E + C 0 47A 2 -74,-2.6 2,-0.3 -2,-0.4 -72,-0.2 -0.896 42.1 127.9-103.0 109.0 14.7 -9.5 33.8 84 351 A M E - 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