==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=31-DEC-2009 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER TRANSCRIPTION 12-MAY-06 2GZW . COMPND 2 MOLECULE: CATABOLITE GENE ACTIVATOR; . SOURCE 2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI; . AUTHOR T.S.KUMAREVEL,T.TANAKA,A.SHINKAI,S.YOKOYAMA,RIKEN . 799 4 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 39903.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 585 73.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 2 0.3 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 183 22.9 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 1 0.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 4 0.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 5 0.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 45 5.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 66 8.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 263 32.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 7 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 3 5 0 4 0 3 2 3 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 RESIDUES PER ALPHA HELIX . 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 6 8 6 6 4 7 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 7 A T 0 0 172 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 173.1 -12.9 12.4 24.8 2 8 A D > - 0 0 53 1,-0.1 4,-2.9 2,-0.0 5,-0.3 -0.950 360.0-155.7-118.1 107.1 -11.5 13.5 21.4 3 9 A P H > S+ 0 0 105 0, 0.0 4,-1.8 0, 0.0 -1,-0.1 0.857 93.2 47.0 -47.2 -46.4 -13.2 16.7 20.1 4 10 A T H > S+ 0 0 22 2,-0.2 4,-2.8 1,-0.2 5,-0.2 0.922 114.0 45.3 -65.8 -48.3 -12.4 15.9 16.4 5 11 A L H > S+ 0 0 32 1,-0.2 4,-3.1 2,-0.2 5,-0.2 0.947 115.9 46.9 -61.9 -48.5 -13.4 12.3 16.5 6 12 A E H X S+ 0 0 102 -4,-2.9 4,-1.0 1,-0.2 -1,-0.2 0.839 111.6 52.4 -62.8 -33.2 -16.6 13.1 18.3 7 13 A W H < S+ 0 0 62 -4,-1.8 3,-0.5 -5,-0.3 -1,-0.2 0.950 112.9 44.5 -65.7 -47.9 -17.2 15.9 15.9 8 14 A F H >< S+ 0 0 1 -4,-2.8 3,-1.7 1,-0.2 -2,-0.2 0.893 108.9 55.3 -62.8 -44.6 -16.7 13.5 13.0 9 15 A L H >< S+ 0 0 28 -4,-3.1 3,-1.2 1,-0.3 -1,-0.2 0.751 96.2 67.0 -64.2 -22.4 -18.9 10.7 14.5 10 16 A S T 3< S+ 0 0 84 -4,-1.0 -1,-0.3 -3,-0.5 -2,-0.2 0.518 100.2 50.2 -76.0 -6.0 -21.8 13.1 14.8 11 17 A H T < S+ 0 0 68 -3,-1.7 -1,-0.3 -4,-0.2 2,-0.2 0.310 103.3 78.8-109.5 1.7 -22.1 13.3 11.0 12 18 A C S < S- 0 0 20 -3,-1.2 2,-0.5 -4,-0.2 79,-0.2 -0.629 72.5-135.4-106.2 165.3 -22.0 9.5 10.7 13 19 A H E -A 90 0A 94 77,-1.9 77,-1.7 -2,-0.2 2,-0.2 -0.996 24.9-137.3-123.6 126.7 -24.7 6.9 11.2 14 20 A I E +A 89 0A 70 -2,-0.5 2,-0.3 75,-0.2 75,-0.2 -0.581 24.5 173.3 -88.1 145.7 -23.7 3.8 13.2 15 21 A H E -A 88 0A 82 73,-2.1 73,-2.4 -2,-0.2 2,-0.4 -0.941 23.6-121.7-144.1 164.8 -24.6 0.2 12.4 16 22 A K E -A 87 0A 173 -2,-0.3 71,-0.2 71,-0.2 69,-0.0 -0.885 15.2-168.5-117.6 146.0 -23.8 -3.2 13.7 17 23 A Y E -A 86 0A 50 69,-2.3 69,-3.5 -2,-0.4 3,-0.1 -0.997 19.4-134.5-130.7 124.6 -22.3 -6.3 12.0 18 24 A P > - 0 0 86 0, 0.0 3,-1.8 0, 0.0 64,-0.2 -0.411 45.2 -74.4 -76.5 158.0 -22.2 -9.7 13.6 19 25 A S T 3 S+ 0 0 58 1,-0.3 64,-0.2 -2,-0.1 65,-0.1 -0.116 121.0 23.2 -47.4 146.4 -19.0 -11.8 13.5 20 26 A K T 3 S+ 0 0 176 62,-3.0 -1,-0.3 1,-0.2 2,-0.1 0.521 91.2 137.7 70.3 6.9 -18.3 -13.3 10.0 21 27 A S < - 0 0 26 -3,-1.8 61,-2.6 63,-0.1 2,-0.7 -0.478 55.0-127.3 -79.8 155.5 -20.4 -10.6 8.3 22 28 A T E +D 81 0B 49 59,-0.2 59,-0.2 1,-0.2 3,-0.1 -0.913 27.0 177.1-109.0 108.5 -19.1 -9.0 5.1 23 29 A L E S+ 0 0 9 57,-1.6 2,-0.4 -2,-0.7 -1,-0.2 0.853 72.0 26.2 -75.6 -40.2 -19.1 -5.2 5.4 24 30 A I E -D 80 0B 4 56,-1.2 56,-2.1 4,-0.0 2,-0.4 -0.995 68.5-154.2-132.3 131.0 -17.5 -4.5 2.0 25 31 A H > - 0 0 85 -2,-0.4 3,-1.6 54,-0.2 51,-0.3 -0.865 27.9-114.6-104.1 133.4 -17.6 -6.7 -1.1 26 32 A Q T 3 S+ 0 0 80 -2,-0.4 51,-0.2 1,-0.2 3,-0.1 -0.410 102.8 28.0 -64.7 140.7 -14.8 -6.4 -3.7 27 33 A G T 3 S+ 0 0 35 49,-2.3 -1,-0.2 1,-0.3 3,-0.1 0.131 93.8 128.7 95.4 -20.1 -16.1 -5.0 -7.0 28 34 A E S < S- 0 0 76 -3,-1.6 48,-2.6 1,-0.1 -1,-0.3 -0.224 73.3 -81.7 -68.9 156.8 -18.9 -3.1 -5.3 29 35 A K B -G 75 0C 126 46,-0.2 2,-0.7 -3,-0.1 46,-0.2 -0.446 40.9-142.6 -61.6 117.4 -19.6 0.6 -5.8 30 36 A A + 0 0 10 44,-2.4 44,-0.4 -2,-0.3 43,-0.3 -0.746 55.8 120.2 -87.1 114.3 -17.2 2.7 -3.6 31 37 A E + 0 0 115 -2,-0.7 62,-2.3 1,-0.2 63,-0.4 0.325 57.8 65.9-152.5 -0.4 -19.1 5.7 -2.4 32 38 A T E -B 92 0A 20 60,-0.2 2,-0.4 -3,-0.2 60,-0.2 -0.927 61.3-143.3-131.9 154.4 -19.0 5.5 1.4 33 39 A L E -B 91 0A 0 58,-2.9 58,-2.0 -2,-0.3 2,-0.3 -0.897 17.0-154.9-115.6 145.1 -16.4 5.7 4.1 34 40 A Y E -BC 90 64A 22 30,-2.7 30,-2.6 -2,-0.4 2,-0.4 -0.863 9.8-166.5-122.1 156.8 -16.3 3.6 7.3 35 41 A Y E -BC 89 63A 28 54,-2.1 54,-2.9 -2,-0.3 2,-0.8 -0.944 24.0-129.8-143.7 117.1 -14.9 4.0 10.8 36 42 A I E +B 88 0A 0 26,-2.6 25,-2.6 -2,-0.4 52,-0.2 -0.540 27.3 173.9 -73.4 106.1 -14.6 1.1 13.3 37 43 A V E S- 0 0 39 50,-2.3 2,-0.3 -2,-0.8 51,-0.2 0.847 74.3 -2.2 -77.8 -36.5 -16.1 2.2 16.6 38 44 A K E S+B 87 0A 130 49,-1.8 49,-2.6 21,-0.1 -1,-0.3 -0.983 106.3 31.6-154.5 146.1 -15.7 -1.3 18.1 39 45 A G - 0 0 17 -2,-0.3 21,-0.5 47,-0.2 2,-0.3 -0.451 68.5 -90.7 104.5-178.0 -14.6 -4.6 16.8 40 46 A S - 0 0 15 44,-0.2 43,-2.3 19,-0.2 44,-1.4 -0.978 31.0-171.1-139.5 150.6 -12.0 -6.0 14.4 41 47 A V E -EF 58 82B 0 17,-2.5 17,-2.2 -2,-0.3 2,-0.4 -0.900 17.6-129.1-138.7 167.0 -12.1 -7.0 10.7 42 48 A A E -EF 57 81B 6 39,-2.5 39,-2.6 -2,-0.3 2,-0.6 -0.928 12.9-144.2-118.7 141.1 -10.0 -8.7 8.0 43 49 A V E -EF 56 80B 24 13,-2.7 12,-3.2 -2,-0.4 13,-1.1 -0.943 30.8-175.8-106.5 119.7 -9.2 -7.3 4.6 44 50 A L E -EF 54 79B 2 35,-3.7 35,-3.2 -2,-0.6 2,-0.3 -0.870 21.8-175.8-123.4 153.8 -9.2 -10.0 2.0 45 51 A I E -E 53 0B 15 8,-1.8 8,-2.4 -2,-0.3 2,-0.3 -0.883 17.5-149.4-133.8 163.3 -8.4 -10.6 -1.7 46 52 A K E -E 52 0B 84 -2,-0.3 6,-0.2 6,-0.2 2,-0.1 -0.968 9.7-128.6-140.5 154.0 -8.8 -13.8 -3.7 47 53 A D > - 0 0 23 4,-1.7 3,-1.4 -2,-0.3 285,-0.0 -0.314 52.1 -83.9 -88.6 179.8 -7.3 -15.7 -6.7 48 54 A E T 3 S+ 0 0 208 1,-0.3 -1,-0.1 2,-0.1 -2,-0.0 0.650 127.4 48.1 -58.6 -16.0 -9.4 -17.1 -9.6 49 55 A E T 3 S- 0 0 152 2,-0.1 -1,-0.3 0, 0.0 3,-0.1 -0.007 121.5 -91.3-120.0 31.7 -10.2 -20.2 -7.5 50 56 A G S < S+ 0 0 46 -3,-1.4 2,-0.3 1,-0.3 -2,-0.1 0.410 71.2 147.6 81.4 -3.4 -11.2 -19.0 -4.0 51 57 A K - 0 0 104 1,-0.0 -4,-1.7 280,-0.0 2,-0.4 -0.523 37.4-147.3 -68.2 128.1 -7.9 -19.0 -2.3 52 58 A E E -E 46 0B 58 -2,-0.3 2,-0.4 -6,-0.2 -6,-0.2 -0.803 15.8-168.5-104.2 139.5 -8.0 -16.2 0.2 53 59 A M E -E 45 0B 1 -8,-2.4 -8,-1.8 -2,-0.4 2,-0.6 -0.986 20.0-133.7-124.4 135.2 -5.2 -13.9 1.3 54 60 A I E -E 44 0B 5 114,-2.1 -10,-0.2 -2,-0.4 3,-0.1 -0.783 19.6-177.9 -88.5 119.5 -5.4 -11.6 4.3 55 61 A L E - 0 0 14 -12,-3.2 2,-0.3 -2,-0.6 -1,-0.2 0.846 64.5 -25.6 -83.3 -37.2 -4.0 -8.2 3.4 56 62 A S E -E 43 0B 40 -13,-1.1 -13,-2.7 2,-0.0 2,-0.5 -0.995 47.8-120.7-169.1 165.7 -4.6 -6.8 6.9 57 63 A Y E -E 42 0B 32 -2,-0.3 2,-0.3 -15,-0.2 -15,-0.2 -0.968 31.2-166.2-113.7 128.8 -6.4 -6.8 10.2 58 64 A L E -E 41 0B 8 -17,-2.2 -17,-2.5 -2,-0.5 2,-0.2 -0.874 10.3-179.0-115.4 152.7 -8.1 -3.5 11.0 59 65 A N > - 0 0 58 -2,-0.3 3,-2.5 -19,-0.2 -23,-0.3 -0.669 39.7 -37.5-136.7-169.8 -9.4 -2.6 14.4 60 66 A Q T 3 S+ 0 0 130 -21,-0.5 -23,-0.2 1,-0.3 3,-0.1 -0.358 125.8 26.6 -57.6 121.0 -11.2 -0.0 16.6 61 67 A G T 3 S+ 0 0 21 -25,-2.6 -1,-0.3 1,-0.4 2,-0.2 0.074 95.2 118.5 110.7 -21.7 -10.1 3.4 15.5 62 68 A D < - 0 0 40 -3,-2.5 -26,-2.6 -26,-0.2 -1,-0.4 -0.555 59.2-134.2 -81.7 141.0 -9.2 2.4 11.9 63 69 A F E -C 35 0A 4 -2,-0.2 2,-0.3 -28,-0.2 -28,-0.3 -0.566 22.9-163.1 -85.3 155.3 -11.0 3.8 8.9 64 70 A I E +C 34 0A 4 -30,-2.6 -30,-2.7 -2,-0.2 16,-0.0 -0.940 62.1 25.1-135.9 159.8 -12.2 1.4 6.2 65 71 A G S S+ 0 0 12 -2,-0.3 3,-0.4 -32,-0.2 -1,-0.1 0.806 73.1 133.3 57.3 35.8 -13.2 1.9 2.6 66 72 A E > + 0 0 7 1,-0.2 3,-1.7 2,-0.1 4,-0.3 0.682 45.5 85.2 -87.7 -17.8 -11.2 5.1 2.2 67 73 A L T 3 S+ 0 0 21 1,-0.3 3,-0.2 2,-0.1 -1,-0.2 0.633 89.3 47.9 -63.0 -18.0 -9.6 4.1 -1.2 68 74 A G T > S+ 0 0 4 -3,-0.4 3,-0.9 -36,-0.2 -1,-0.3 0.497 87.0 96.9 -99.0 -2.4 -12.5 5.3 -3.4 69 75 A L T < S+ 0 0 0 -3,-1.7 -1,-0.1 1,-0.2 -2,-0.1 0.739 82.8 41.6 -57.3 -35.4 -12.7 8.7 -1.6 70 76 A F T 3 S+ 0 0 27 -4,-0.3 2,-0.4 -3,-0.2 -1,-0.2 0.493 116.7 45.0 -98.1 -0.8 -10.7 10.9 -3.9 71 77 A E S X S- 0 0 93 -3,-0.9 3,-0.5 -4,-0.1 -1,-0.2 -0.966 75.2-138.3-145.2 124.0 -12.0 9.6 -7.2 72 78 A E T 3 S+ 0 0 161 -2,-0.4 -41,-0.1 1,-0.2 -3,-0.0 -0.313 81.7 41.0 -75.7 161.8 -15.5 8.9 -8.2 73 79 A G T 3 S+ 0 0 86 -43,-0.3 2,-0.3 1,-0.2 -1,-0.2 0.653 83.4 133.9 76.6 16.4 -16.6 5.8 -10.2 74 80 A Q < - 0 0 42 -3,-0.5 -44,-2.4 -44,-0.4 -1,-0.2 -0.737 45.0-139.9-102.4 150.4 -14.3 3.4 -8.3 75 81 A E B -G 29 0C 130 -2,-0.3 -46,-0.2 -46,-0.2 2,-0.1 -0.635 36.1 -83.4-102.0 163.2 -15.1 -0.0 -6.9 76 82 A R - 0 0 17 -48,-2.6 -49,-2.3 -51,-0.3 -1,-0.1 -0.414 32.2-150.1 -65.4 135.1 -13.9 -1.6 -3.7 77 83 A S S S+ 0 0 46 -51,-0.2 2,-0.3 -2,-0.1 -1,-0.1 0.471 70.3 7.4 -87.7 -3.4 -10.5 -3.1 -4.0 78 84 A A S S- 0 0 7 -53,-0.1 2,-0.4 -33,-0.1 -33,-0.2 -0.971 76.6 -93.9-164.6 174.2 -11.0 -5.9 -1.5 79 85 A W E - F 0 44B 48 -35,-3.2 -35,-3.7 -2,-0.3 2,-0.5 -0.805 28.4-156.8 -99.6 139.9 -13.4 -7.8 0.9 80 86 A V E -DF 24 43B 7 -56,-2.1 -57,-1.6 -2,-0.4 -56,-1.2 -0.982 12.9-173.0-120.9 120.9 -13.7 -6.7 4.5 81 87 A R E -DF 22 42B 74 -39,-2.6 -39,-2.5 -2,-0.5 2,-0.3 -0.960 26.0-119.2-121.0 128.8 -15.0 -9.4 6.9 82 88 A A E - F 0 41B 1 -61,-2.6 -62,-3.0 -2,-0.4 -41,-0.3 -0.437 21.5-174.2 -64.5 122.1 -15.9 -8.9 10.6 83 89 A K S S+ 0 0 77 -43,-2.3 2,-0.3 -2,-0.3 -42,-0.2 0.835 78.6 11.9 -83.7 -36.6 -13.7 -11.2 12.8 84 90 A T S S- 0 0 65 -44,-1.4 -44,-0.2 -65,-0.1 -1,-0.2 -0.827 110.8 -64.8-130.5 169.1 -15.7 -10.2 15.9 85 91 A A - 0 0 69 -2,-0.3 2,-0.4 -66,-0.1 -45,-0.2 -0.316 64.0-151.9 -55.2 138.0 -19.1 -8.3 16.3 86 92 A C E -A 17 0A 6 -69,-3.5 -69,-2.3 -47,-0.2 2,-0.6 -0.923 25.2-158.7-125.6 146.9 -18.5 -4.8 15.0 87 93 A E E -AB 16 38A 73 -49,-2.6 -50,-2.3 -2,-0.4 -49,-1.8 -0.970 29.1-176.4-117.4 112.4 -19.9 -1.3 15.5 88 94 A V E -AB 15 36A 0 -73,-2.4 -73,-2.1 -2,-0.6 2,-0.4 -0.925 22.2-145.3-120.7 138.6 -19.1 0.8 12.5 89 95 A A E -AB 14 35A 0 -54,-2.9 -54,-2.1 -2,-0.4 2,-0.4 -0.802 21.7-166.7 -97.0 136.9 -19.7 4.5 11.6 90 96 A E E +AB 13 34A 18 -77,-1.7 -77,-1.9 -2,-0.4 2,-0.3 -0.967 12.7 169.4-128.3 142.6 -20.5 5.2 8.0 91 97 A I E - B 0 33A 3 -58,-2.0 -58,-2.9 -2,-0.4 -79,-0.1 -0.989 37.3-104.6-151.2 141.5 -20.6 8.5 6.1 92 98 A S E > - B 0 32A 39 -2,-0.3 4,-2.2 -60,-0.2 -60,-0.2 -0.314 27.6-121.2 -65.3 150.9 -20.9 9.3 2.4 93 99 A Y H > S+ 0 0 8 -62,-2.3 4,-2.1 1,-0.2 5,-0.2 0.903 113.1 60.0 -59.0 -37.0 -17.8 10.4 0.5 94 100 A K H > S+ 0 0 118 -63,-0.4 4,-1.2 1,-0.2 -1,-0.2 0.911 107.7 41.6 -56.7 -49.2 -19.7 13.6 -0.3 95 101 A K H > S+ 0 0 80 1,-0.2 4,-2.2 2,-0.2 3,-0.4 0.919 111.0 55.6 -67.7 -43.1 -20.2 14.6 3.3 96 102 A F H X S+ 0 0 0 -4,-2.2 4,-2.0 1,-0.3 -1,-0.2 0.861 104.9 54.8 -57.0 -37.7 -16.7 13.6 4.4 97 103 A R H X S+ 0 0 70 -4,-2.1 4,-1.3 2,-0.2 -1,-0.3 0.866 106.9 49.9 -64.4 -36.6 -15.3 15.9 1.7 98 104 A Q H X S+ 0 0 108 -4,-1.2 4,-1.4 -3,-0.4 -2,-0.2 0.897 109.2 52.1 -68.5 -38.4 -17.3 18.8 3.2 99 105 A L H X S+ 0 0 9 -4,-2.2 4,-2.7 1,-0.2 -2,-0.2 0.860 101.1 62.9 -65.4 -34.4 -16.0 18.0 6.6 100 106 A I H < S+ 0 0 1 -4,-2.0 -1,-0.2 1,-0.2 -2,-0.2 0.937 102.4 48.4 -55.9 -49.4 -12.4 18.1 5.2 101 107 A Q H < S+ 0 0 98 -4,-1.3 -1,-0.2 1,-0.2 -2,-0.2 0.889 111.6 51.9 -59.5 -37.2 -12.8 21.8 4.4 102 108 A V H < S- 0 0 103 -4,-1.4 -2,-0.2 1,-0.2 -1,-0.2 0.947 135.2 -2.6 -64.3 -49.8 -14.1 22.4 7.8 103 109 A N >< - 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