==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=11-JAN-2013 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 14-SEP-12 4H3L . COMPND 2 MOLECULE: FLUORESCENT PROTEIN PLUM; . SOURCE 2 ORGANISM_SCIENTIFIC: DISCOSOMA SP. LW-2004; . AUTHOR M.M.MOORE,R.A.CHICA . 425 5 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 17488.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 310 72.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 24 5.6 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 200 47.1 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 6 1.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 37 8.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 33 7.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 12 2.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 3 0.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 2 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 2 2 0 4 2 2 2 4 4 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 7 A V 0 0 103 0, 0.0 2,-0.8 0, 0.0 107,-0.0 0.000 360.0 360.0 360.0 -19.6 -3.5 -27.6 -5.3 2 8 A I - 0 0 16 77,-0.1 2,-0.1 4,-0.0 4,-0.1 -0.887 360.0-163.7 -98.7 108.7 -5.8 -24.6 -4.4 3 9 A K - 0 0 100 -2,-0.8 29,-0.2 2,-0.2 3,-0.0 -0.347 35.1-103.9 -85.3 168.6 -9.1 -25.3 -6.1 4 10 A E S S+ 0 0 155 1,-0.2 2,-0.4 26,-0.1 29,-0.2 0.744 114.8 42.6 -66.2 -24.9 -11.8 -22.7 -6.7 5 11 A F S S+ 0 0 94 25,-0.2 2,-0.4 26,-0.1 -2,-0.2 -0.996 75.1 169.7-121.6 127.1 -13.7 -24.3 -3.8 6 12 A M E -A 29 0A 3 23,-2.3 23,-1.5 -2,-0.4 2,-0.2 -1.000 20.8-149.4-140.3 136.1 -11.9 -25.3 -0.6 7 13 A R E -A 28 0A 99 101,-0.5 103,-2.2 -2,-0.4 2,-0.3 -0.595 15.9-162.3 -93.0 161.3 -12.9 -26.5 2.8 8 14 A F E -Ab 27 110A 2 19,-1.9 19,-2.6 101,-0.2 103,-0.2 -0.993 12.5-150.6-145.8 152.2 -10.9 -25.8 6.0 9 15 A K E - b 0 111A 40 101,-2.4 103,-2.2 -2,-0.3 2,-0.3 -0.932 17.1-165.3-127.5 107.8 -10.8 -27.2 9.5 10 16 A P E - b 0 112A 8 0, 0.0 15,-1.0 0, 0.0 2,-0.4 -0.716 9.0-172.9 -94.8 139.3 -9.8 -25.0 12.3 11 17 A H E -Cb 24 113A 53 101,-2.6 103,-3.0 -2,-0.3 2,-0.4 -1.000 11.8-179.0-122.9 130.4 -8.7 -26.0 15.8 12 18 A M E +Cb 23 114A 2 11,-2.9 11,-2.5 -2,-0.4 2,-0.3 -0.989 6.8 163.3-133.9 130.3 -8.2 -23.2 18.3 13 19 A E E +Cb 22 115A 75 101,-2.4 103,-2.5 -2,-0.4 2,-0.3 -0.925 20.8 93.9-136.1 164.1 -7.1 -23.4 21.9 14 20 A G E -Cb 21 116A 9 7,-1.7 7,-2.2 -2,-0.3 2,-0.3 -0.994 56.3 -83.5 155.2-152.5 -5.7 -21.0 24.5 15 21 A S E -Cb 20 117A 5 101,-1.8 103,-3.1 -2,-0.3 2,-0.4 -0.994 14.3-157.7-151.8 149.5 -6.8 -18.8 27.4 16 22 A V E > S-Cb 19 118A 0 3,-1.8 3,-1.7 -2,-0.3 103,-0.2 -0.974 87.1 -14.4-131.2 119.4 -8.1 -15.3 28.0 17 23 A N T 3 S- 0 0 51 101,-2.4 102,-0.1 -2,-0.4 -1,-0.1 0.901 131.8 -51.3 53.4 43.0 -7.7 -13.8 31.4 18 24 A G T 3 S+ 0 0 49 100,-0.5 2,-0.8 1,-0.2 -1,-0.3 0.332 109.6 126.4 82.4 -8.0 -6.9 -17.3 32.8 19 25 A H E < -C 16 0A 60 -3,-1.7 -3,-1.8 30,-0.0 -1,-0.2 -0.752 48.8-152.4 -88.8 110.6 -10.0 -18.9 31.2 20 26 A E E +C 15 0A 141 -2,-0.8 2,-0.3 -5,-0.2 -5,-0.2 -0.455 22.4 162.4 -82.5 151.4 -8.9 -21.8 29.1 21 27 A F E -C 14 0A 6 -7,-2.2 -7,-1.7 -2,-0.1 2,-0.3 -0.976 26.6-148.4-157.3 165.5 -10.9 -23.1 26.1 22 28 A E E -CD 13 44A 81 22,-2.1 22,-1.6 -2,-0.3 21,-1.6 -0.986 14.8-172.2-140.6 147.5 -10.9 -25.1 23.0 23 29 A I E +CD 12 42A 2 -11,-2.5 -11,-2.9 -2,-0.3 2,-0.3 -0.991 4.5 177.4-141.3 141.0 -12.8 -24.6 19.8 24 30 A E E +CD 11 41A 101 17,-2.3 17,-2.9 -2,-0.3 2,-0.3 -0.882 13.9 136.1-133.5 170.3 -13.3 -26.6 16.6 25 31 A G E - D 0 40A 23 -15,-1.0 2,-0.3 -2,-0.3 15,-0.2 -0.975 38.0-109.3 175.8-164.9 -15.2 -26.3 13.4 26 32 A E E + D 0 39A 73 13,-1.9 13,-3.1 -2,-0.3 2,-0.3 -0.979 27.5 179.2-153.3 144.3 -15.4 -26.5 9.7 27 33 A G E -AD 8 38A 1 -19,-2.6 -19,-1.9 -2,-0.3 2,-0.3 -0.869 10.5-159.0-141.2 173.9 -15.6 -23.9 6.9 28 34 A E E +AD 7 37A 56 9,-2.2 9,-2.8 -21,-0.3 2,-0.3 -0.981 20.7 143.3-151.8 158.4 -15.7 -23.5 3.1 29 35 A G E -AD 6 36A 7 -23,-1.5 -23,-2.3 -2,-0.3 7,-0.3 -0.984 49.5 -93.3-177.8-176.1 -15.0 -20.8 0.7 30 36 A R E >>> - D 0 35A 152 5,-2.3 3,-1.6 -2,-0.3 5,-1.4 -0.911 29.4-163.9-123.0 96.5 -13.7 -19.6 -2.7 31 37 A P T 345S+ 0 0 0 0, 0.0 34,-0.3 0, 0.0 3,-0.2 0.826 88.6 49.0 -58.2 -34.5 -10.0 -18.7 -2.3 32 38 A Y T 345S+ 0 0 109 1,-0.2 35,-0.2 -28,-0.2 -28,-0.1 0.625 112.1 49.4 -81.2 -13.2 -9.8 -16.7 -5.5 33 39 A E T <45S- 0 0 90 -3,-1.6 33,-0.3 -29,-0.2 -1,-0.2 0.583 107.1-126.5 -95.9 -13.0 -12.9 -14.7 -4.7 34 40 A G T <5 + 0 0 1 -4,-1.2 175,-2.3 1,-0.2 2,-0.3 0.759 67.3 124.9 75.5 27.0 -11.7 -13.9 -1.2 35 41 A T E < +DE 30 208A 29 -5,-1.4 -5,-2.3 173,-0.2 2,-0.3 -0.918 26.9 153.7-125.9 146.6 -14.8 -15.3 0.4 36 42 A Q E -DE 29 207A 10 171,-1.5 171,-2.3 -2,-0.3 2,-0.3 -0.990 20.8-157.4-164.3 154.0 -15.4 -17.9 3.1 37 43 A T E -DE 28 206A 45 -9,-2.8 -9,-2.2 -2,-0.3 2,-0.3 -0.933 12.7-167.9-130.7 160.2 -17.8 -19.1 5.9 38 44 A A E -DE 27 205A 2 167,-2.0 167,-1.9 -2,-0.3 2,-0.5 -0.999 19.1-145.1-150.4 148.3 -17.3 -21.2 9.0 39 45 A R E -DE 26 204A 52 -13,-3.1 -13,-1.9 -2,-0.3 2,-0.3 -0.970 28.7-171.6-109.0 120.8 -19.2 -23.1 11.6 40 46 A L E -DE 25 203A 2 163,-3.5 163,-2.3 -2,-0.5 2,-0.4 -0.842 9.6-171.0-117.1 150.0 -17.5 -23.0 15.0 41 47 A K E -DE 24 202A 52 -17,-2.9 -17,-2.3 -2,-0.3 2,-0.7 -1.000 24.2-131.1-137.0 138.4 -18.1 -24.8 18.3 42 48 A V E +D 23 0A 20 159,-2.5 -19,-0.3 -2,-0.4 3,-0.1 -0.800 28.8 173.8 -87.4 117.0 -16.7 -24.4 21.8 43 49 A T E + 0 0 74 -21,-1.6 2,-0.3 -2,-0.7 -20,-0.2 0.630 64.7 29.5-101.2 -19.8 -15.8 -27.9 22.8 44 50 A K E S+D 22 0A 35 -22,-1.6 -22,-2.1 1,-0.1 -1,-0.3 -0.994 118.8 16.1-143.1 137.1 -14.0 -27.1 26.1 45 51 A G S S- 0 0 33 -2,-0.3 -2,-0.1 1,-0.3 -1,-0.1 0.598 88.6-170.7 82.7 12.6 -14.4 -24.2 28.6 46 52 A G + 0 0 41 -4,-0.1 -1,-0.3 2,-0.1 2,-0.1 -0.585 57.4 48.9 -86.5 150.5 -17.8 -23.1 27.3 47 53 A P S S- 0 0 81 0, 0.0 153,-0.1 0, 0.0 154,-0.1 0.513 86.6-138.0 -77.0 142.9 -19.6 -20.9 27.8 48 54 A L - 0 0 8 151,-0.5 4,-0.1 152,-0.1 -2,-0.1 -0.477 1.1-146.3 -66.7 136.2 -16.8 -18.3 27.3 49 55 A P S S+ 0 0 48 0, 0.0 2,-0.3 0, 0.0 -1,-0.1 0.103 78.4 59.4 -89.8 18.7 -16.9 -15.5 29.9 50 56 A F S S- 0 0 3 79,-0.1 78,-0.2 80,-0.1 2,-0.1 -0.931 100.1 -68.8-142.1 167.9 -15.5 -13.0 27.3 51 57 A A > - 0 0 4 76,-1.8 3,-1.4 -2,-0.3 4,-0.4 -0.318 27.7-145.7 -62.5 127.7 -16.5 -11.6 23.8 52 58 A W G >> S+ 0 0 8 1,-0.2 3,-2.1 2,-0.2 4,-1.5 0.826 92.8 78.4 -53.9 -34.5 -16.4 -14.1 20.9 53 59 A D G 34 S+ 0 0 9 1,-0.3 -1,-0.2 2,-0.2 -2,-0.1 0.732 83.9 58.9 -54.3 -26.9 -15.3 -11.1 18.8 54 60 A I G <4 S+ 0 0 0 -3,-1.4 -1,-0.3 1,-0.2 -2,-0.2 0.782 111.8 41.6 -75.0 -19.6 -11.7 -11.2 20.1 55 61 A L T X4 S+ 0 0 0 -3,-2.1 3,-2.4 -4,-0.4 4,-0.2 0.717 93.2 82.7 -93.8 -27.4 -11.4 -14.8 18.8 56 62 A S G >< S+ 0 0 0 -4,-1.5 3,-0.9 1,-0.3 -2,-0.1 0.819 89.7 50.4 -54.7 -37.4 -13.1 -14.4 15.4 57 63 A P G 3 S+ 0 0 19 0, 0.0 -1,-0.3 0, 0.0 -2,-0.1 0.505 98.3 67.0 -78.8 -0.9 -10.2 -12.9 13.5 58 64 A Q G < 0 0 0 -3,-2.4 -2,-0.2 1,-0.2 -3,-0.1 0.293 360.0 360.0 -95.3 7.7 -7.8 -15.8 14.7 59 65 A I < 0 0 9 -3,-0.9 -1,-0.2 -4,-0.2 -3,-0.1 -0.015 360.0 360.0-112.9 360.0 -9.9 -18.1 12.6 60 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 61 69 A S > 0 0 11 0, 0.0 3,-1.1 0, 0.0 -2,-0.1 0.000 360.0 360.0 360.0 77.2 -7.7 -17.6 7.0 62 70 A K T 3 + 0 0 16 1,-0.3 3,-0.3 13,-0.0 14,-0.2 0.463 360.0 78.9 -92.0 -1.9 -5.5 -15.8 4.5 63 71 A A T 3 S+ 0 0 0 1,-0.2 2,-0.8 12,-0.1 -1,-0.3 0.681 87.7 69.4 -68.4 -17.7 -5.5 -18.2 1.7 64 72 A Y < + 0 0 0 -3,-1.1 2,-0.4 -30,-0.1 -1,-0.2 -0.527 70.0 115.0-103.1 64.9 -9.0 -16.7 1.0 65 73 A V - 0 0 4 -2,-0.8 2,-0.3 -34,-0.3 145,-0.2 -0.991 65.0-125.5-130.6 125.8 -8.0 -13.2 -0.2 66 74 A K B -f 210 0A 80 143,-2.3 145,-3.1 -2,-0.4 -34,-0.1 -0.540 33.0-172.9 -70.2 126.5 -8.7 -12.1 -3.7 67 75 A H - 0 0 31 -2,-0.3 145,-0.1 143,-0.2 6,-0.1 -0.967 23.7-122.6-120.2 139.5 -5.5 -10.9 -5.4 68 76 A P > - 0 0 47 0, 0.0 3,-1.7 0, 0.0 144,-0.0 -0.417 37.0-106.3 -69.2 160.4 -5.1 -9.2 -8.7 69 77 A A T 3 S+ 0 0 113 1,-0.3 4,-0.1 -2,-0.1 -2,-0.0 0.750 115.9 59.8 -59.2 -28.0 -2.6 -11.0 -11.0 70 78 A D T 3 S+ 0 0 109 1,-0.1 -1,-0.3 2,-0.1 -3,-0.0 0.404 92.5 72.3 -90.9 5.1 0.1 -8.4 -10.5 71 79 A I S < S- 0 0 9 -3,-1.7 -1,-0.1 111,-0.1 140,-0.1 -0.964 89.9-122.0-117.2 113.7 0.4 -8.9 -6.8 72 80 A P - 0 0 27 0, 0.0 2,-1.6 0, 0.0 3,-0.2 -0.323 14.6-137.5 -57.4 131.2 2.0 -12.2 -5.8 73 81 A D >> + 0 0 25 1,-0.2 4,-2.8 -4,-0.1 3,-0.6 -0.578 30.0 176.8 -92.2 71.0 -0.3 -14.2 -3.6 74 82 A Y H 3> S+ 0 0 6 -2,-1.6 4,-1.1 1,-0.3 -1,-0.2 0.814 73.5 50.2 -44.4 -44.7 2.4 -15.3 -1.2 75 83 A L H 34 S+ 0 0 9 1,-0.2 -1,-0.3 2,-0.2 4,-0.2 0.863 112.9 44.5 -75.7 -35.7 0.1 -17.1 1.1 76 84 A K H X4 S+ 0 0 15 -3,-0.6 3,-1.7 1,-0.2 -1,-0.2 0.903 111.6 55.5 -65.3 -41.7 -1.6 -19.2 -1.6 77 85 A L H 3< S+ 0 0 86 -4,-2.8 -2,-0.2 1,-0.3 -1,-0.2 0.745 93.3 70.7 -62.2 -23.5 1.8 -19.9 -3.2 78 86 A S T 3X S+ 0 0 6 -4,-1.1 4,-2.7 -5,-0.2 3,-0.4 0.662 88.0 141.0 -70.3 -16.5 3.0 -21.3 0.1 79 87 A F T <4 + 0 0 16 -3,-1.7 -77,-0.1 1,-0.3 25,-0.0 -0.488 45.1 25.4 -83.2 152.0 0.7 -24.3 -0.4 80 88 A P T 4 S+ 0 0 78 0, 0.0 25,-2.0 0, 0.0 -1,-0.3 -0.964 133.1 38.5 -81.9 -13.7 0.6 -27.2 -0.0 81 89 A E T 4 S- 0 0 92 -3,-0.4 2,-0.3 1,-0.2 -2,-0.3 0.950 101.0-164.4 -61.3 -45.5 3.2 -27.0 2.8 82 90 A G < - 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