==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=11-JAN-2013 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 14-SEP-12 4H3M . COMPND 2 MOLECULE: FLUORESCENT PROTEIN PLUM; . SOURCE 2 ORGANISM_SCIENTIFIC: DISCOSOMA SP. LW-2004; . AUTHOR M.M.MOORE,R.A.CHICA . 423 5 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 17436.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 309 73.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 24 5.7 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 200 47.3 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 3 0.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 6 1.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 40 9.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 27 6.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 14 3.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 2 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 2 0 0 4 0 2 2 6 2 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 6 A E 0 0 132 0, 0.0 3,-0.1 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0-135.6 -13.5 -23.3 -9.8 2 7 A V + 0 0 71 1,-0.2 2,-0.8 78,-0.1 107,-0.0 0.884 360.0 56.3 -75.3 -37.8 -13.1 -23.9 -6.1 3 8 A I S S- 0 0 10 77,-0.1 -1,-0.2 4,-0.0 2,-0.1 -0.866 79.8-163.5 -98.3 108.3 -15.3 -21.0 -5.0 4 9 A K - 0 0 91 -2,-0.8 29,-0.1 2,-0.2 -2,-0.0 -0.375 34.4-104.7 -84.3 166.7 -18.7 -21.5 -6.6 5 10 A E S S+ 0 0 159 1,-0.2 2,-0.4 26,-0.1 28,-0.2 0.720 113.3 39.3 -69.1 -19.2 -21.4 -18.9 -6.9 6 11 A F S S+ 0 0 92 25,-0.2 2,-0.4 26,-0.1 25,-0.2 -0.996 75.2 172.6-129.1 127.2 -23.4 -20.6 -4.1 7 12 A M E -A 30 0A 7 23,-2.0 23,-1.5 -2,-0.4 2,-0.2 -0.998 18.8-149.6-136.0 139.5 -21.5 -22.1 -1.1 8 13 A R E -A 29 0A 112 101,-0.5 103,-2.4 -2,-0.4 2,-0.3 -0.635 15.3-161.1 -96.9 160.7 -22.6 -23.6 2.2 9 14 A F E -Ab 28 111A 5 19,-1.9 19,-2.6 101,-0.2 2,-0.4 -0.984 11.7-151.3-140.1 152.8 -20.7 -23.4 5.5 10 15 A K E -Ab 27 112A 31 101,-2.5 103,-3.0 -2,-0.3 2,-0.4 -0.983 18.8-165.7-119.3 137.4 -20.6 -25.3 8.8 11 16 A A E -Ab 26 113A 7 15,-2.4 15,-2.5 -2,-0.4 2,-0.4 -0.966 12.8-174.1-129.4 138.9 -19.5 -23.5 11.9 12 17 A H E -Ab 25 114A 69 101,-2.4 103,-3.0 -2,-0.4 2,-0.4 -0.999 11.7-172.7-128.5 128.7 -18.4 -24.7 15.3 13 18 A M E +Ab 24 115A 1 11,-3.5 11,-3.0 -2,-0.4 2,-0.3 -0.980 6.4 178.9-128.7 132.9 -17.7 -22.2 18.1 14 19 A E E +Ab 23 116A 121 101,-2.8 103,-2.6 -2,-0.4 2,-0.3 -0.943 23.5 110.6-124.7 150.3 -16.4 -22.7 21.6 15 20 A G E -Ab 22 117A 11 7,-1.8 7,-2.2 -2,-0.3 2,-0.3 -1.000 46.9-107.4 174.1-165.3 -15.6 -20.2 24.3 16 21 A S E -Ab 21 118A 29 101,-2.1 103,-2.7 -2,-0.3 2,-0.4 -0.992 16.2-167.2-152.1 131.7 -16.1 -18.5 27.5 17 22 A V E > S-Ab 20 119A 0 3,-1.9 3,-2.1 -2,-0.3 103,-0.2 -0.972 81.0 -17.2-130.7 123.0 -17.4 -15.0 28.4 18 23 A N T 3 S- 0 0 57 101,-2.3 102,-0.2 -2,-0.4 -1,-0.1 0.884 132.9 -50.3 48.9 40.5 -16.9 -13.5 31.9 19 24 A G T 3 S+ 0 0 56 100,-0.6 2,-0.8 1,-0.2 -1,-0.3 0.369 110.4 127.5 83.9 -3.5 -16.1 -17.1 32.9 20 25 A H E < -A 17 0A 60 -3,-2.1 -3,-1.9 30,-0.0 -1,-0.2 -0.776 47.8-150.7 -91.5 113.1 -19.3 -18.6 31.3 21 26 A E E +A 16 0A 149 -2,-0.8 2,-0.3 -5,-0.2 -5,-0.3 -0.459 24.5 157.7 -83.1 150.5 -18.4 -21.5 29.0 22 27 A F E -A 15 0A 12 -7,-2.2 -7,-1.8 -2,-0.1 2,-0.3 -0.981 29.4-141.5-161.3 164.7 -20.5 -22.4 25.9 23 28 A E E -AC 14 45A 56 22,-2.2 21,-1.6 -2,-0.3 22,-1.5 -0.999 14.0-169.7-134.4 139.4 -20.6 -24.2 22.6 24 29 A I E -AC 13 43A 1 -11,-3.0 -11,-3.5 -2,-0.3 2,-0.4 -0.993 1.3-172.4-130.4 135.3 -22.4 -23.1 19.4 25 30 A E E +AC 12 42A 90 17,-2.2 17,-2.4 -2,-0.4 2,-0.3 -0.968 16.5 163.2-123.5 144.4 -22.9 -25.1 16.2 26 31 A G E -AC 11 41A 16 -15,-2.5 -15,-2.4 -2,-0.4 2,-0.3 -0.970 27.6-147.9-154.4 164.7 -24.4 -23.7 13.0 27 32 A E E +AC 10 40A 76 13,-2.2 13,-3.0 -2,-0.3 2,-0.2 -0.995 23.7 168.5-136.5 145.0 -24.9 -24.0 9.3 28 33 A G E -AC 9 39A 0 -19,-2.6 -19,-1.9 -2,-0.3 2,-0.3 -0.823 15.7-157.8-143.1-177.8 -25.2 -21.3 6.6 29 34 A E E +AC 8 38A 53 9,-2.3 9,-2.5 -21,-0.3 2,-0.3 -0.973 20.5 143.5-159.6 157.8 -25.2 -20.5 2.9 30 35 A G E -AC 7 37A 6 -23,-1.5 -23,-2.0 -2,-0.3 7,-0.3 -0.978 49.6 -89.7-177.5-168.2 -24.6 -17.6 0.6 31 36 A R E >>> - C 0 36A 152 5,-2.6 4,-1.4 -2,-0.3 5,-1.3 -0.890 30.0-164.5-131.9 91.9 -23.3 -16.1 -2.6 32 37 A P T 345S+ 0 0 0 0, 0.0 34,-0.3 0, 0.0 -1,-0.1 0.766 88.0 49.3 -54.5 -34.8 -19.6 -15.3 -2.2 33 38 A Y T 345S+ 0 0 98 -28,-0.2 35,-0.2 1,-0.2 -28,-0.1 0.695 113.7 46.4 -80.3 -17.7 -19.4 -13.0 -5.2 34 39 A E T <45S- 0 0 107 -3,-1.3 33,-0.3 -29,-0.2 -1,-0.2 0.610 107.1-126.1 -98.2 -10.9 -22.5 -11.1 -4.2 35 40 A G T <5 + 0 0 0 -4,-1.4 175,-2.5 1,-0.2 2,-0.3 0.737 66.3 121.4 79.4 26.1 -21.4 -10.6 -0.6 36 41 A T E < +CD 31 209A 33 -5,-1.3 -5,-2.6 173,-0.2 2,-0.3 -0.917 26.8 153.1-128.2 146.9 -24.5 -12.1 1.0 37 42 A Q E -CD 30 208A 14 171,-1.4 171,-2.4 -2,-0.3 2,-0.3 -0.973 19.8-159.0-165.9 154.9 -25.1 -15.0 3.4 38 43 A T E -CD 29 207A 47 -9,-2.5 -9,-2.3 -2,-0.3 2,-0.3 -0.937 12.5-170.8-134.4 159.5 -27.5 -16.3 6.1 39 44 A A E -CD 28 206A 3 167,-2.0 167,-2.0 -2,-0.3 2,-0.5 -0.995 20.8-139.3-150.7 156.9 -27.0 -18.9 8.9 40 45 A R E -CD 27 205A 57 -13,-3.0 -13,-2.2 -2,-0.3 2,-0.3 -0.979 27.9-171.6-114.8 124.2 -28.9 -20.8 11.5 41 46 A L E -CD 26 204A 4 163,-2.9 163,-2.1 -2,-0.5 2,-0.4 -0.896 8.2-167.1-120.3 147.5 -27.1 -21.1 14.9 42 47 A K E -CD 25 203A 39 -17,-2.4 -17,-2.2 -2,-0.3 2,-0.9 -0.999 24.5-129.7-134.6 131.7 -27.9 -23.2 18.0 43 48 A V E +C 24 0A 17 159,-2.2 -19,-0.2 -2,-0.4 4,-0.2 -0.723 30.6 177.1 -78.1 106.4 -26.4 -23.0 21.5 44 49 A T E + 0 0 65 -21,-1.6 2,-0.3 -2,-0.9 -20,-0.2 0.737 64.3 20.5 -88.3 -26.9 -25.5 -26.6 22.1 45 50 A K E S+C 23 0A 47 -22,-1.5 -22,-2.2 1,-0.1 -1,-0.2 -0.997 120.6 22.6-140.9 141.8 -23.9 -26.2 25.5 46 51 A G S S- 0 0 32 -2,-0.3 -2,-0.1 1,-0.3 -1,-0.1 0.575 90.4-173.6 85.4 9.4 -24.1 -23.5 28.1 47 52 A G + 0 0 41 -4,-0.2 -1,-0.3 2,-0.1 2,-0.1 -0.596 55.1 46.5 -89.7 152.8 -27.5 -22.2 27.0 48 53 A P S S- 0 0 75 0, 0.0 153,-0.1 0, 0.0 154,-0.1 0.512 91.9-131.4 -77.7 145.0 -29.3 -20.1 27.5 49 54 A L - 0 0 23 151,-0.3 -2,-0.1 152,-0.2 4,-0.1 -0.306 2.2-142.0 -66.8 139.6 -26.4 -17.7 27.4 50 55 A P S S+ 0 0 56 0, 0.0 2,-0.3 0, 0.0 -1,-0.1 0.296 81.5 55.8 -82.7 14.0 -26.1 -15.2 30.3 51 56 A F S S- 0 0 8 79,-0.1 78,-0.2 80,-0.1 2,-0.2 -0.911 100.0 -69.6-141.9 168.2 -24.9 -12.5 27.9 52 57 A A > - 0 0 5 76,-2.1 3,-1.6 -2,-0.3 4,-0.3 -0.378 29.4-146.1 -64.4 125.3 -25.9 -10.7 24.7 53 58 A W G >> S+ 0 0 20 1,-0.3 3,-2.5 -2,-0.2 4,-1.6 0.774 90.7 82.4 -53.4 -29.9 -25.8 -12.8 21.6 54 59 A D G 34 S+ 0 0 13 1,-0.3 -1,-0.3 2,-0.2 82,-0.1 0.738 83.4 57.4 -54.2 -28.2 -24.7 -9.6 19.8 55 60 A I G <4 S+ 0 0 0 -3,-1.6 -1,-0.3 1,-0.2 -2,-0.2 0.714 110.6 44.3 -76.3 -17.1 -21.1 -10.1 20.8 56 61 A L T X> S+ 0 0 0 -3,-2.5 3,-1.6 -4,-0.3 4,-1.2 0.747 93.2 78.5 -95.9 -30.1 -21.0 -13.5 19.2 57 62 A S T 3< S+ 0 0 1 -4,-1.6 3,-0.3 1,-0.3 -2,-0.1 0.831 92.6 50.4 -51.9 -42.6 -22.7 -12.7 15.9 58 63 A P T 34 S+ 0 0 17 0, 0.0 -1,-0.3 0, 0.0 -2,-0.1 0.684 103.0 62.2 -71.9 -14.2 -19.7 -11.1 14.3 59 64 A Q T <4 0 0 1 -3,-1.6 -2,-0.2 1,-0.2 -3,-0.1 0.833 360.0 360.0 -86.6 -16.3 -17.5 -14.1 15.2 60 65 A I < 0 0 13 -4,-1.2 -1,-0.2 -3,-0.3 -3,-0.1 0.869 360.0 360.0 -86.5 360.0 -19.7 -16.3 13.0 61 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 62 69 A S > 0 0 15 0, 0.0 3,-1.7 0, 0.0 -2,-0.1 0.000 360.0 360.0 360.0 64.7 -17.3 -15.4 7.2 63 70 A K T 3 + 0 0 11 1,-0.3 3,-0.4 2,-0.1 14,-0.2 0.456 360.0 82.0 -79.1 -4.5 -15.1 -13.3 5.0 64 71 A A T 3 S+ 0 0 0 1,-0.2 2,-0.6 12,-0.1 -1,-0.3 0.692 87.4 65.3 -67.6 -15.3 -15.1 -15.5 2.0 65 72 A Y < + 0 0 0 -3,-1.7 2,-0.5 -30,-0.1 145,-0.3 -0.526 69.6 120.4-107.8 63.5 -18.5 -13.9 1.2 66 73 A V - 0 0 2 -2,-0.6 2,-0.4 -3,-0.4 145,-0.2 -0.992 62.4-125.9-125.9 121.9 -17.5 -10.2 0.5 67 74 A K B -e 211 0A 73 143,-2.2 145,-3.0 -2,-0.5 -34,-0.1 -0.551 34.1-171.8 -67.4 125.5 -18.3 -8.7 -2.9 68 75 A H - 0 0 30 -2,-0.4 145,-0.1 -35,-0.2 6,-0.1 -0.919 25.6-112.9-117.5 145.0 -15.1 -7.3 -4.4 69 76 A P > - 0 0 42 0, 0.0 3,-1.4 0, 0.0 144,-0.0 -0.342 36.6-111.2 -63.2 159.2 -14.5 -5.1 -7.5 70 77 A A T 3 S+ 0 0 113 1,-0.3 4,-0.1 3,-0.1 -2,-0.0 0.854 115.4 57.9 -60.9 -36.6 -12.6 -6.9 -10.3 71 78 A D T 3 S+ 0 0 142 2,-0.1 -1,-0.3 1,-0.1 0, 0.0 0.321 92.6 78.5 -84.1 9.3 -9.5 -4.7 -9.8 72 79 A I S < S- 0 0 29 -3,-1.4 2,-0.1 111,-0.1 -1,-0.1 -0.953 86.0-126.7-115.1 112.2 -9.2 -5.7 -6.1 73 80 A P - 0 0 28 0, 0.0 2,-1.7 0, 0.0 3,-0.2 -0.347 16.5-135.2 -56.9 128.8 -7.6 -9.1 -5.5 74 81 A D > - 0 0 23 1,-0.2 4,-2.6 -4,-0.1 3,-0.3 -0.620 28.3-178.6 -89.4 81.3 -9.9 -11.1 -3.3 75 82 A Y H > S+ 0 0 4 -2,-1.7 4,-0.8 1,-0.2 -1,-0.2 0.826 75.3 48.8 -54.0 -40.5 -7.2 -12.4 -1.0 76 83 A L H 4 S+ 0 0 7 1,-0.2 4,-0.3 2,-0.2 -1,-0.2 0.887 113.4 46.0 -77.3 -35.3 -9.4 -14.5 1.2 77 84 A K H >4 S+ 0 0 12 -3,-0.3 3,-1.5 1,-0.2 -2,-0.2 0.901 109.8 56.2 -63.6 -43.9 -11.2 -16.2 -1.7 78 85 A L H 3< S+ 0 0 82 -4,-2.6 -2,-0.2 1,-0.2 -1,-0.2 0.732 94.0 71.1 -59.9 -21.1 -7.8 -16.8 -3.4 79 86 A S T 3X S+ 0 0 1 -4,-0.8 4,-2.4 -3,-0.2 3,-0.4 0.671 86.0 132.5 -72.4 -18.5 -6.7 -18.6 -0.2 80 87 A F T <4 + 0 0 9 -3,-1.5 -78,-0.1 -4,-0.3 -77,-0.1 -0.408 45.5 33.8 -83.1 158.2 -8.9 -21.6 -0.9 81 88 A P T 4 S+ 0 0 61 0, 0.0 25,-1.2 0, 0.0 -1,-0.3 -0.973 134.3 37.1 -73.1 -19.8 -9.0 -24.6 -1.0 82 89 A E T 4 S- 0 0 106 -3,-0.4 -2,-0.3 1,-0.2 2,-0.3 0.936 100.4-165.3 -59.8 -51.9 -6.5 -24.4 1.9 83 90 A G < - 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