==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=3-DEC-2009 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER LUMINESCENT PROTEIN 22-JUN-01 1H6R . COMPND 2 MOLECULE: GREEN FLUORESCENT PROTEIN; . SOURCE 2 ORGANISM_SCIENTIFIC: AEQUOREA VICTORIA; . AUTHOR H.OSTERGAARD,A.HENRIKSEN,F.G.HANSEN,J.R.WINTHER . 681 6 3 3 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 29427.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 495 72.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 36 5.3 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 286 42.0 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 6 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 6 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 6 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 71 10.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 70 10.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 17 2.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 9 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 3 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 2 0 0 3 3 0 3 4 7 4 5 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 2 A S 0 0 93 0, 0.0 2,-0.4 0, 0.0 619,-0.1 0.000 360.0 360.0 360.0-145.9 4.3 29.8 33.4 2 3 A K > - 0 0 32 84,-0.1 3,-1.2 604,-0.0 4,-0.1 -0.987 360.0 -26.2-135.7 142.7 4.3 31.3 30.0 3 4 A G G > S+ 0 0 0 82,-2.8 3,-2.1 -2,-0.4 4,-0.2 -0.199 121.5 1.6 64.7-141.8 7.1 32.8 27.8 4 5 A E G > S+ 0 0 31 1,-0.3 3,-2.2 2,-0.2 -1,-0.2 0.730 117.3 75.1 -58.5 -24.0 10.2 34.3 29.1 5 6 A E G X S+ 0 0 32 -3,-1.2 3,-1.1 1,-0.3 -1,-0.3 0.811 86.7 64.8 -58.7 -26.9 9.2 33.6 32.7 6 7 A L G < S+ 0 0 33 -3,-2.1 -1,-0.3 1,-0.2 3,-0.2 0.616 102.3 49.3 -70.4 -11.3 10.1 29.9 32.0 7 8 A F G < S+ 0 0 4 -3,-2.2 29,-0.4 -4,-0.2 30,-0.4 0.233 72.7 106.0-114.1 13.0 13.8 30.9 31.5 8 9 A T S < S+ 0 0 48 -3,-1.1 2,-0.2 -4,-0.2 -1,-0.1 0.715 87.1 26.3 -69.8 -18.8 14.5 33.1 34.6 9 10 A G S S- 0 0 34 -3,-0.2 27,-0.5 -4,-0.2 2,-0.5 -0.401 111.3 -53.0-121.6-158.9 16.6 30.4 36.2 10 11 A V E -A 35 0A 74 25,-0.2 25,-0.2 -2,-0.2 -2,-0.1 -0.720 54.9-160.1 -84.8 125.2 18.7 27.4 35.1 11 12 A V E -A 34 0A 10 23,-2.9 23,-2.4 -2,-0.5 104,-0.1 -0.930 14.9-126.4-113.3 124.5 16.7 25.1 32.9 12 13 A P E -A 33 0A 65 0, 0.0 104,-2.6 0, 0.0 2,-0.4 -0.400 27.8-152.3 -66.2 144.4 17.6 21.5 32.2 13 14 A I E -Ab 32 116A 5 19,-2.6 19,-1.7 102,-0.2 2,-0.4 -0.954 14.2-168.8-123.3 138.9 17.8 20.6 28.6 14 15 A L E -Ab 31 117A 48 102,-2.4 104,-2.6 -2,-0.4 2,-0.4 -0.993 8.2-172.5-122.0 130.2 17.3 17.3 26.7 15 16 A V E +Ab 30 118A 1 15,-2.6 15,-3.0 -2,-0.4 2,-0.4 -0.985 3.9 179.7-122.5 135.1 18.4 17.0 23.1 16 17 A E E -Ab 29 119A 72 102,-2.6 104,-3.6 -2,-0.4 2,-0.4 -0.997 2.2-176.9-134.2 132.4 17.6 13.9 20.9 17 18 A L E -Ab 28 120A 1 11,-2.7 11,-2.2 -2,-0.4 2,-0.5 -0.993 14.2-166.0-134.2 129.7 18.5 13.5 17.3 18 19 A D E -Ab 27 121A 106 102,-2.3 104,-2.7 -2,-0.4 2,-0.3 -0.965 27.9-175.3-107.4 125.8 17.8 10.7 14.8 19 20 A G E -Ab 26 122A 8 7,-3.3 7,-2.2 -2,-0.5 2,-0.3 -0.872 27.8-177.7-129.2 159.9 20.0 11.0 11.8 20 21 A D E +Ab 25 123A 33 102,-1.0 104,-3.7 -2,-0.3 2,-0.5 -0.877 10.1 177.5-151.6 111.0 20.7 9.5 8.4 21 22 A V E > S-Ab 24 124A 0 3,-2.5 3,-1.9 -2,-0.3 104,-0.2 -0.961 77.4 -22.8-123.4 112.3 23.6 10.8 6.3 22 23 A N T 3 S- 0 0 57 102,-2.9 -1,-0.1 -2,-0.5 103,-0.1 0.867 127.9 -51.3 58.4 37.5 24.2 9.0 3.0 23 24 A G T 3 S+ 0 0 49 101,-0.4 2,-0.6 1,-0.3 -1,-0.3 0.066 109.3 126.9 89.5 -25.4 22.4 6.0 4.3 24 25 A H E < -A 21 0A 96 -3,-1.9 -3,-2.5 29,-0.1 2,-0.3 -0.571 48.2-156.2 -71.0 110.6 24.5 5.9 7.5 25 26 A K E +A 20 0A 160 -2,-0.6 2,-0.3 -5,-0.2 -5,-0.2 -0.651 26.0 142.5 -92.4 146.7 22.0 5.8 10.3 26 27 A F E -A 19 0A 26 -7,-2.2 -7,-3.3 -2,-0.3 2,-0.3 -0.977 29.4-145.2-169.3 165.9 22.7 7.0 13.8 27 28 A S E -A 18 0A 29 -2,-0.3 21,-2.7 -9,-0.2 22,-0.3 -0.957 7.4-160.0-141.8 162.2 21.3 8.8 16.8 28 29 A V E -AC 17 47A 2 -11,-2.2 -11,-2.7 -2,-0.3 2,-0.4 -0.996 2.3-159.7-144.1 142.8 22.6 11.1 19.5 29 30 A S E -AC 16 46A 60 17,-2.2 17,-2.9 -2,-0.3 2,-0.3 -0.966 13.6-176.2-123.6 141.0 21.3 12.1 22.9 30 31 A G E -AC 15 45A 4 -15,-3.0 -15,-2.6 -2,-0.4 2,-0.3 -0.955 5.3-179.5-135.4 154.3 22.4 15.3 24.8 31 32 A E E +AC 14 44A 141 13,-2.0 13,-2.4 -2,-0.3 2,-0.3 -0.988 24.8 105.4-149.6 153.8 21.7 16.7 28.2 32 33 A G E -AC 13 43A 27 -19,-1.7 -19,-2.6 -2,-0.3 2,-0.3 -0.943 52.9 -85.2 171.6-146.4 22.7 19.8 30.0 33 34 A E E -AC 12 42A 92 9,-2.3 9,-2.3 -2,-0.3 2,-0.4 -0.993 18.0-144.0-155.7 156.6 21.3 23.1 31.1 34 35 A G E -AC 11 41A 0 -23,-2.4 -23,-2.9 -2,-0.3 2,-0.4 -0.968 4.6-169.5-125.7 144.9 20.6 26.7 29.9 35 36 A D E > > -AC 10 40A 33 5,-3.0 5,-2.2 -2,-0.4 3,-1.7 -0.819 5.2-176.1-133.2 89.3 20.8 29.9 31.8 36 37 A A G > 5S+ 0 0 7 -27,-0.5 3,-0.7 -2,-0.4 35,-0.1 0.731 76.2 75.4 -63.9 -22.1 19.3 32.6 29.6 37 38 A T G 3 5S+ 0 0 75 -30,-0.4 -1,-0.3 1,-0.3 -29,-0.1 0.853 114.0 25.9 -57.2 -31.3 20.1 35.3 32.1 38 39 A Y G < 5S- 0 0 146 -3,-1.7 -1,-0.3 2,-0.1 -2,-0.2 0.281 110.8-117.9-110.7 5.6 23.7 34.9 30.8 39 40 A G T < 5 + 0 0 0 -3,-0.7 182,-2.4 -4,-0.3 2,-0.4 0.783 65.6 153.7 61.0 29.7 22.8 33.5 27.4 40 41 A K E < -CD 35 220A 86 -5,-2.2 -5,-3.0 180,-0.2 2,-0.4 -0.766 29.5-179.4-106.9 132.6 24.6 30.4 28.4 41 42 A L E -CD 34 219A 0 178,-2.8 178,-2.6 -2,-0.4 2,-0.4 -0.981 2.3-175.8-123.4 135.2 24.2 26.8 27.3 42 43 A T E +CD 33 218A 55 -9,-2.3 -9,-2.3 -2,-0.4 2,-0.3 -0.986 20.3 161.8-131.2 117.9 26.2 23.9 28.6 43 44 A L E -CD 32 217A 4 174,-2.7 174,-3.2 -2,-0.4 2,-0.4 -0.980 29.0-155.6-140.6 152.2 25.6 20.5 26.9 44 45 A K E -CD 31 216A 41 -13,-2.4 -13,-2.0 -2,-0.3 2,-0.4 -0.999 14.1-163.1-127.5 132.0 27.1 17.1 26.5 45 46 A F E -CD 30 215A 0 170,-3.1 170,-2.1 -2,-0.4 2,-0.4 -0.930 4.3-171.9-116.8 135.4 26.2 14.9 23.5 46 47 A I E -CD 29 214A 32 -17,-2.9 -17,-2.2 -2,-0.4 168,-0.2 -0.993 25.3-124.3-128.6 129.1 26.8 11.2 23.3 47 48 A V E -C 28 0A 5 166,-2.2 -19,-0.3 -2,-0.4 3,-0.1 -0.476 26.6-121.8 -68.2 144.8 26.3 9.1 20.1 48 49 A T S S- 0 0 82 -21,-2.7 2,-0.3 1,-0.2 -1,-0.1 0.903 84.1 -18.6 -55.8 -43.6 23.9 6.2 20.9 49 50 A T S S- 0 0 117 -22,-0.3 -1,-0.2 2,-0.0 -22,-0.2 -0.983 109.6 -3.7-160.3 156.8 26.5 3.6 19.8 50 51 A G S S- 0 0 57 -2,-0.3 163,-0.6 -3,-0.1 2,-0.4 -0.269 102.0 -23.2 64.1-139.1 29.8 3.3 17.8 51 52 A K - 0 0 140 161,-0.1 -2,-0.0 -2,-0.0 -3,-0.0 -0.889 61.4-113.7-115.6 139.6 31.2 6.3 16.0 52 53 A L - 0 0 1 -2,-0.4 4,-0.1 1,-0.1 -26,-0.1 -0.548 23.7-141.2 -67.6 133.2 29.4 9.3 14.7 53 54 A P S S+ 0 0 26 0, 0.0 83,-0.2 0, 0.0 -1,-0.1 0.567 82.3 42.5 -74.0 -11.3 29.5 9.3 10.8 54 55 A V S S- 0 0 1 81,-0.1 2,-0.1 82,-0.0 80,-0.1 -0.878 102.6 -82.9-129.4 163.0 30.0 13.0 10.5 55 56 A P > - 0 0 9 0, 0.0 3,-1.4 0, 0.0 4,-0.4 -0.440 35.2-126.4 -69.8 137.7 32.2 15.5 12.4 56 57 A W G >> S+ 0 0 12 1,-0.3 3,-2.1 2,-0.2 4,-0.5 0.873 106.4 62.8 -49.7 -44.3 30.7 16.7 15.7 57 58 A P G >4 S+ 0 0 4 0, 0.0 3,-1.1 0, 0.0 4,-0.4 0.834 93.2 64.2 -55.2 -29.7 31.2 20.4 14.7 58 59 A T G <4 S+ 0 0 2 -3,-1.4 -2,-0.2 1,-0.2 4,-0.2 0.654 103.6 47.4 -70.0 -11.6 28.8 19.9 11.8 59 60 A L G <> S+ 0 0 0 -3,-2.1 4,-2.9 -4,-0.4 -1,-0.2 0.443 81.5 97.3-106.0 -2.1 25.9 19.2 14.1 60 61 A V T << S+ 0 0 4 -3,-1.1 -2,-0.1 -4,-0.5 -1,-0.1 0.934 86.6 45.7 -54.4 -49.7 26.4 22.1 16.6 61 62 A T T 4 S+ 0 0 23 -4,-0.4 -1,-0.2 1,-0.2 -2,-0.1 0.836 113.9 49.7 -66.2 -29.0 23.8 24.3 15.0 62 63 A T T 4 0 0 3 -3,-0.2 -1,-0.2 1,-0.2 -2,-0.2 0.922 360.0 360.0 -73.3 -43.2 21.3 21.5 14.7 63 64 A F < 0 0 7 -4,-2.9 -2,-0.2 -48,-0.1 -1,-0.2 0.837 360.0 360.0 -67.9 360.0 21.8 20.5 18.4 64 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 65 68 A L > 0 0 15 0, 0.0 3,-2.1 0, 0.0 4,-0.1 0.000 360.0 360.0 360.0 53.7 18.3 26.2 21.4 66 69 A Q G > + 0 0 6 1,-0.3 3,-1.8 2,-0.2 16,-0.2 0.647 360.0 80.8 -63.3 -14.2 17.9 29.9 20.5 67 70 A C G 3 S+ 0 0 0 1,-0.3 -1,-0.3 14,-0.2 -28,-0.1 0.632 83.9 64.3 -67.1 -11.2 15.5 30.3 23.4 68 71 A F G < S+ 0 0 1 -3,-2.1 153,-0.3 -29,-0.1 -1,-0.3 0.397 74.9 124.1 -94.6 8.6 18.5 30.5 25.7 69 72 A A < - 0 0 1 -3,-1.8 2,-0.9 1,-0.1 -30,-0.2 -0.242 67.3-119.5 -63.2 148.2 19.8 33.7 24.2 70 73 A R B -e 222 0A 89 151,-2.7 153,-2.7 -31,-0.2 -33,-0.1 -0.826 27.9-165.1 -89.1 109.3 20.3 36.7 26.4 71 74 A Y - 0 0 2 -2,-0.9 5,-0.1 151,-0.2 153,-0.1 -0.879 24.8-121.0 -94.1 125.0 18.1 39.5 25.1 72 75 A P > - 0 0 17 0, 0.0 3,-2.3 0, 0.0 4,-0.2 -0.128 30.3 -96.6 -58.1 163.6 19.2 42.8 26.7 73 76 A D T 3 S+ 0 0 76 1,-0.3 3,-0.3 2,-0.2 530,-0.1 0.649 123.6 51.6 -60.5 -18.2 16.7 44.7 28.8 74 77 A H T 3 S+ 0 0 36 1,-0.2 -1,-0.3 152,-0.1 3,-0.1 0.448 109.0 51.4 -98.3 2.9 15.7 47.0 25.9 75 78 A M X + 0 0 1 -3,-2.3 3,-2.0 1,-0.1 -2,-0.2 0.205 66.0 114.7-122.3 17.5 15.0 44.1 23.5 76 79 A K G > S+ 0 0 43 -3,-0.3 3,-1.4 1,-0.3 528,-0.1 0.818 73.1 62.0 -61.1 -28.6 12.6 41.8 25.4 77 80 A R G 3 S+ 0 0 60 1,-0.3 -1,-0.3 -3,-0.1 -2,-0.1 0.588 98.9 59.2 -73.5 -5.5 9.8 42.4 23.0 78 81 A H G < S+ 0 0 5 -3,-2.0 2,-1.3 115,-0.1 -1,-0.3 0.268 72.7 109.2-105.0 9.9 11.9 40.9 20.2 79 82 A D <> + 0 0 7 -3,-1.4 4,-1.4 1,-0.2 112,-0.3 -0.674 33.4 166.4 -93.7 90.0 12.4 37.5 21.8 80 83 A F H > + 0 0 1 -2,-1.3 4,-1.3 1,-0.2 111,-0.3 0.921 68.4 61.4 -64.9 -44.7 10.4 35.2 19.6 81 84 A F H 4 S+ 0 0 0 1,-0.2 3,-0.5 2,-0.2 4,-0.2 0.883 108.9 37.1 -55.7 -50.9 11.9 32.0 20.9 82 85 A K H >4 S+ 0 0 13 1,-0.2 3,-2.0 -16,-0.2 -1,-0.2 0.869 108.7 65.9 -71.4 -31.6 11.0 32.3 24.6 83 86 A S H 3< S+ 0 0 2 -4,-1.4 -1,-0.2 1,-0.3 -2,-0.2 0.759 95.1 58.5 -61.8 -23.8 7.6 33.8 23.7 84 87 A A T 3X S+ 0 0 5 -4,-1.3 4,-2.3 -3,-0.5 -1,-0.3 0.577 92.1 146.3 -79.9 -9.6 6.5 30.5 22.1 85 88 A M T <4 + 0 0 0 -3,-2.0 -82,-2.8 1,-0.3 -79,-0.1 -0.507 48.7 19.5 -82.3 151.9 7.0 28.5 25.3 86 89 A P T 4 S+ 0 0 45 0, 0.0 25,-2.1 0, 0.0 -1,-0.3 -0.975 130.8 41.7 -80.0 -17.1 5.9 26.2 26.7 87 90 A E T 4 S- 0 0 90 -3,-0.2 -2,-0.2 23,-0.2 2,-0.1 0.853 99.8-166.5 -59.8 -34.9 4.2 24.9 23.6 88 91 A G < - 0 0 1 -4,-2.3 97,-2.7 -6,-0.1 98,-0.6 -0.357 8.9 -82.5 91.6-166.3 7.3 25.7 21.6 89 92 A Y E -FG 109 184A 0 20,-2.1 20,-3.0 95,-0.3 2,-0.5 -0.950 21.5-119.6-145.6 160.7 8.2 25.9 18.0 90 93 A V E -FG 108 183A 16 93,-2.9 93,-2.2 -2,-0.3 2,-0.5 -0.895 25.9-160.9 -99.9 131.3 9.3 23.9 15.0 91 94 A Q E -FG 107 182A 8 16,-2.7 16,-2.3 -2,-0.5 2,-0.4 -0.979 10.7-175.6-115.4 122.5 12.8 24.8 13.6 92 95 A E E +FG 106 181A 75 89,-3.1 89,-2.3 -2,-0.5 2,-0.3 -0.953 5.2 173.4-119.2 139.7 13.5 23.6 10.1 93 96 A R E -FG 105 180A 12 12,-2.1 12,-2.5 -2,-0.4 2,-0.4 -0.973 27.5-158.1-141.1 155.9 16.8 24.0 8.2 94 97 A T E -FG 104 179A 33 85,-1.8 85,-2.4 -2,-0.3 2,-0.5 -0.994 20.5-165.1-124.5 129.9 18.5 23.1 5.1 95 98 A I E -FG 103 178A 0 8,-2.8 8,-2.1 -2,-0.4 2,-0.6 -0.965 1.8-160.8-119.3 114.5 22.3 23.3 5.5 96 99 A F E -FG 102 177A 91 81,-2.9 81,-3.1 -2,-0.5 2,-0.7 -0.853 6.2-152.1 -99.4 116.7 24.4 23.3 2.3 97 100 A F E > - G 0 176A 4 4,-2.5 3,-2.3 -2,-0.6 79,-0.2 -0.805 33.4-109.4 -88.2 120.1 28.0 22.3 2.7 98 101 A K T 3 S+ 0 0 130 77,-2.0 3,-0.1 -2,-0.7 77,-0.1 -0.216 98.4 0.1 -49.9 128.1 29.9 24.1 -0.2 99 102 A D T 3 S+ 0 0 135 1,-0.2 -1,-0.3 75,-0.1 2,-0.2 0.684 131.0 62.7 63.4 19.7 31.1 21.6 -2.8 100 103 A D S < S- 0 0 29 -3,-2.3 -1,-0.2 1,-0.3 27,-0.2 -0.714 91.1 -52.2-149.2-160.2 29.6 18.7 -0.8 101 104 A G - 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