==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=3-DEC-2009 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER ANTIBODY 14-FEB-01 1H8O . COMPND 2 MOLECULE: MUTANT AL2 6E7P9G; . SOURCE 2 ORGANISM_SCIENTIFIC: MUS MUSCULUS; . AUTHOR J.BURMESTER,S.SPINELLI,L.PUGLIESE,A.KREBBER,A.HONEGGER, . 436 4 4 4 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 21878.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 290 66.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 20 4.6 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 157 36.0 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 5 1.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 1 0.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 45 10.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 43 9.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 8 1.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 2 0.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 8 5 4 2 11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 2 2 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 4 A D 0 0 141 0, 0.0 2,-0.7 0, 0.0 95,-0.1 0.000 360.0 360.0 360.0 117.8 15.7 46.4 -0.9 2 5 A I - 0 0 5 93,-0.4 2,-0.4 91,-0.1 88,-0.1 -0.750 360.0-153.9 -91.3 115.8 15.3 48.3 2.3 3 6 A V - 0 0 83 -2,-0.7 23,-1.3 94,-0.1 2,-0.6 -0.666 1.7-157.1 -88.0 135.2 17.7 51.2 2.8 4 7 A L E -A 25 0A 11 94,-0.5 2,-0.6 -2,-0.4 96,-0.5 -0.948 8.2-160.4-113.6 118.3 18.5 52.4 6.3 5 8 A T E -A 24 0A 72 19,-2.3 19,-2.4 -2,-0.6 2,-0.5 -0.886 3.5-152.7-105.4 123.2 19.8 55.9 6.5 6 9 A Q E -A 23 0A 1 -2,-0.6 17,-0.2 17,-0.2 322,-0.1 -0.821 19.5-126.5 -97.2 126.4 21.7 57.0 9.6 7 10 A S S S+ 0 0 11 15,-1.7 2,-0.3 -2,-0.5 -1,-0.1 0.811 83.2 7.2 -28.0 -70.3 21.6 60.7 10.5 8 11 A H - 0 0 46 14,-0.2 3,-0.1 1,-0.1 -1,-0.1 -0.959 50.7-143.9-131.3 147.2 25.3 61.4 10.7 9 12 A K S S+ 0 0 105 -2,-0.3 94,-3.2 1,-0.2 2,-0.3 0.823 98.0 23.1 -70.9 -33.7 28.6 59.6 10.0 10 13 A F E -d 103 0B 106 92,-0.2 2,-0.4 -3,-0.1 -1,-0.2 -0.998 63.7-173.4-138.1 138.5 30.2 61.3 13.0 11 14 A M E -d 104 0B 24 92,-2.4 94,-1.4 -2,-0.3 2,-0.4 -0.975 14.9-150.2-134.9 117.2 28.6 62.8 16.1 12 15 A S E +d 105 0B 83 -2,-0.4 2,-0.2 92,-0.2 94,-0.2 -0.702 29.7 153.1 -88.0 135.0 30.8 64.8 18.6 13 16 A T E -d 106 0B 9 92,-2.4 94,-2.0 -2,-0.4 2,-0.3 -0.799 30.9-131.4-144.6-172.6 29.7 64.7 22.2 14 17 A S > - 0 0 46 -2,-0.2 3,-2.2 93,-0.2 64,-0.2 -0.993 41.6 -76.5-149.1 145.7 31.2 65.0 25.7 15 18 A V T 3 S+ 0 0 79 -2,-0.3 64,-0.2 1,-0.3 63,-0.1 -0.073 117.3 26.7 -41.8 131.0 30.8 63.0 28.9 16 19 A G T 3 S+ 0 0 43 62,-2.2 -1,-0.3 1,-0.3 63,-0.1 0.186 95.8 123.4 99.4 -16.3 27.5 63.7 30.7 17 20 A D < - 0 0 74 -3,-2.2 61,-2.8 60,-0.2 2,-0.5 -0.216 61.5-121.4 -77.5 166.5 25.6 64.6 27.5 18 21 A R - 0 0 121 59,-0.2 2,-0.3 -3,-0.1 58,-0.2 -0.908 32.8-173.3-108.1 130.5 22.5 63.3 26.0 19 22 A V E - B 0 75A 11 56,-2.1 56,-1.8 -2,-0.5 2,-0.4 -0.949 12.9-158.8-127.8 149.8 22.9 62.0 22.4 20 23 A S E - B 0 74A 33 -2,-0.3 2,-0.6 54,-0.2 54,-0.2 -0.931 1.8-166.2-133.6 114.0 20.4 60.7 19.9 21 24 A I E - B 0 73A 0 52,-3.2 52,-3.2 -2,-0.4 2,-0.3 -0.840 16.0-161.6 -98.7 123.5 21.3 58.5 17.0 22 25 A T E - B 0 72A 9 -2,-0.6 -15,-1.7 50,-0.2 2,-0.3 -0.788 13.3-175.3-110.8 154.5 18.6 58.2 14.3 23 26 A a E -AB 6 71A 0 48,-2.4 48,-2.6 -2,-0.3 2,-0.5 -0.994 6.7-162.3-146.7 129.9 18.0 55.8 11.6 24 27 A K E -AB 5 70A 70 -19,-2.4 -19,-2.3 -2,-0.3 46,-0.2 -0.961 9.1-147.5-122.4 122.6 15.2 55.9 8.9 25 28 A A E -A 4 0A 2 44,-1.8 -21,-0.2 -2,-0.5 4,-0.1 -0.511 16.7-134.3 -82.4 154.6 14.1 53.0 6.8 26 29 A S S S+ 0 0 72 -23,-1.3 2,-0.3 -2,-0.1 -1,-0.1 0.282 91.1 24.5 -89.3 9.4 13.0 53.7 3.2 27 30 A Q S S- 0 0 105 -24,-0.2 2,-0.4 41,-0.2 -2,-0.1 -0.972 105.3 -70.5-162.3 163.7 10.0 51.4 3.9 28 31 A D + 0 0 117 -2,-0.3 41,-0.2 1,-0.2 3,-0.1 -0.456 43.4 168.7 -67.8 120.8 7.9 50.2 6.8 29 32 A V > - 0 0 1 39,-2.5 3,-2.3 -2,-0.4 2,-0.3 0.108 32.5-149.0-114.9 14.5 10.0 47.8 8.9 30 33 A G T 3 - 0 0 27 38,-0.3 38,-3.6 1,-0.3 -1,-0.3 -0.350 61.8 -35.0 60.2-114.5 7.5 47.8 11.7 31 34 A T T 3 S+ 0 0 61 -2,-0.3 2,-1.3 36,-0.2 -1,-0.3 0.068 103.4 116.7-128.5 16.5 9.3 47.3 15.0 32 35 A A < + 0 0 7 -3,-2.3 59,-2.4 18,-0.2 2,-0.4 -0.511 42.8 122.2 -92.5 67.8 12.0 44.8 13.7 33 36 A V E -E 90 0B 0 -2,-1.3 18,-2.5 57,-0.2 2,-0.3 -0.985 38.1-169.1-133.1 135.8 15.1 46.9 14.3 34 37 A A E -E 89 0B 0 55,-2.1 55,-2.2 -2,-0.4 2,-0.4 -0.916 12.4-142.0-124.5 154.5 18.2 46.2 16.4 35 38 A W E -EF 88 48B 0 13,-2.3 12,-2.8 -2,-0.3 13,-1.3 -0.948 15.5-163.9-117.5 135.5 21.0 48.5 17.5 36 39 A Y E -EF 87 46B 6 51,-3.5 51,-3.0 -2,-0.4 2,-0.4 -0.942 10.2-154.6-124.5 145.0 24.7 47.5 17.6 37 40 A Q E -EF 86 45B 11 8,-2.3 8,-2.9 -2,-0.4 2,-0.4 -0.909 14.3-172.5-110.9 136.1 27.8 49.0 19.3 38 41 A Q E -E 85 0B 21 47,-2.4 47,-2.5 -2,-0.4 5,-0.1 -0.964 7.6-163.0-137.0 120.9 31.2 48.3 17.8 39 42 A K > - 0 0 57 -2,-0.4 3,-1.4 4,-0.3 45,-0.1 -0.683 48.4 -74.9 -92.8 153.5 34.5 49.2 19.4 40 43 A P T 3 S+ 0 0 96 0, 0.0 -1,-0.1 0, 0.0 3,-0.1 -0.166 118.0 7.6 -52.8 135.3 37.7 49.2 17.1 41 44 A G T 3 S+ 0 0 37 1,-0.2 2,-0.3 -3,-0.1 71,-0.1 0.820 111.8 109.5 63.2 31.0 39.0 45.7 16.3 42 45 A Q S < S- 0 0 111 -3,-1.4 -1,-0.2 73,-0.0 3,-0.1 -0.976 72.1-109.6-137.9 150.5 35.9 44.2 17.9 43 46 A S - 0 0 38 -2,-0.3 -4,-0.3 1,-0.1 169,-0.1 -0.486 52.2 -86.2 -77.7 146.7 32.8 42.3 16.8 44 47 A P - 0 0 5 0, 0.0 2,-0.4 0, 0.0 166,-0.4 -0.157 39.8-145.9 -50.1 145.4 29.5 44.2 17.1 45 48 A K E -F 37 0B 101 -8,-2.9 -8,-2.3 164,-0.1 2,-0.3 -0.974 17.4-119.5-119.6 129.7 27.7 43.9 20.4 46 49 A L E +F 36 0B 10 -2,-0.4 -10,-0.2 -10,-0.2 12,-0.2 -0.546 38.0 163.6 -72.2 129.9 23.9 43.8 20.5 47 50 A L E + 0 0 1 -12,-2.8 8,-2.3 1,-0.3 2,-0.3 0.757 61.9 10.1-111.6 -44.8 22.3 46.7 22.5 48 51 A I E -FG 35 54B 1 -13,-1.3 -13,-2.3 6,-0.2 -1,-0.3 -0.993 57.0-177.4-146.4 135.6 18.6 46.8 21.6 49 52 A Y E >> + G 0 53B 59 4,-2.5 4,-2.7 -2,-0.3 3,-0.6 -0.903 67.8 33.3-130.3 161.9 16.1 44.6 19.6 50 53 A W T 34 S- 0 0 126 -2,-0.3 2,-1.9 1,-0.3 -18,-0.2 0.886 124.1 -77.6 59.6 39.1 12.5 44.9 18.6 51 54 A A T 34 S+ 0 0 1 -18,-2.5 -1,-0.3 -3,-0.2 21,-0.2 -0.044 127.2 27.7 63.9 -31.5 13.2 48.6 18.4 52 55 A S T <4 S+ 0 0 74 -2,-1.9 2,-0.7 -3,-0.6 12,-0.3 0.400 81.8 111.3-142.0 8.8 13.0 49.1 22.2 53 56 A T E < -G 49 0B 58 -4,-2.7 -4,-2.5 10,-0.1 2,-0.2 -0.788 59.1-138.1 -92.2 116.1 14.0 46.1 24.2 54 57 A R E -G 48 0B 108 -2,-0.7 -6,-0.2 -6,-0.2 2,-0.2 -0.471 23.9-121.6 -68.6 137.6 17.3 46.5 26.0 55 58 A H > - 0 0 51 -8,-2.3 3,-1.5 -2,-0.2 -1,-0.1 -0.542 39.0 -79.3 -84.4 150.8 19.5 43.4 25.8 56 59 A T T 3 S+ 0 0 125 1,-0.3 2,-0.1 -2,-0.2 -1,-0.1 -0.193 119.1 18.4 -47.5 124.8 20.7 41.5 28.8 57 60 A G T 3 S+ 0 0 71 1,-0.3 -1,-0.3 -3,-0.1 -10,-0.1 -0.304 88.8 123.8 107.7 -50.5 23.7 43.4 30.2 58 61 A V < - 0 0 12 -3,-1.5 -1,-0.3 -12,-0.2 -4,-0.0 -0.121 62.2-117.5 -48.7 134.2 23.2 46.8 28.5 59 62 A P > - 0 0 41 0, 0.0 3,-1.9 0, 0.0 -1,-0.1 -0.327 22.3-105.6 -75.4 160.3 23.0 49.7 31.0 60 63 A D T 3 S+ 0 0 139 1,-0.3 16,-0.2 15,-0.0 -2,-0.1 0.870 108.2 82.5 -45.4 -49.7 20.0 52.0 31.6 61 64 A R T 3 S+ 0 0 48 14,-0.1 15,-2.6 16,-0.1 2,-0.4 0.694 81.5 77.4 -28.9 -33.9 21.8 54.8 29.7 62 65 A F E < +C 75 0A 3 -3,-1.9 2,-0.4 13,-0.2 13,-0.2 -0.720 56.1 169.4 -94.2 130.3 20.6 53.2 26.4 63 66 A T E -C 74 0A 71 11,-1.9 11,-3.3 -2,-0.4 2,-0.3 -0.999 13.1-163.2-139.7 135.3 17.1 53.6 25.2 64 67 A G E +C 73 0A 5 -2,-0.4 2,-0.3 -12,-0.3 9,-0.2 -0.827 17.5 159.5-114.5 156.5 15.7 52.6 21.8 65 68 A S E +C 72 0A 61 7,-1.5 7,-3.2 -2,-0.3 2,-0.2 -0.966 15.6 95.7-166.6 162.1 12.4 53.9 20.3 66 69 A G E -C 71 0A 31 -2,-0.3 2,-0.3 5,-0.2 5,-0.2 -0.548 37.0-145.1 131.5 162.0 10.6 54.2 17.0 67 70 A S E > -C 70 0A 90 3,-1.1 2,-2.1 -2,-0.2 3,-0.9 -0.949 63.4 -6.5-163.7 141.7 8.2 52.4 14.8 68 71 A G T 3 S- 0 0 7 -38,-3.6 -39,-2.5 -2,-0.3 -38,-0.3 -0.472 131.7 -7.7 76.0 -78.6 7.5 51.9 11.2 69 72 A T T 3 S+ 0 0 38 -2,-2.1 -44,-1.8 1,-0.2 2,-0.5 0.650 118.6 62.9-129.8 -20.9 10.0 54.2 9.6 70 73 A D E < -BC 24 67A 20 -3,-0.9 -3,-1.1 -46,-0.2 2,-0.4 -0.940 57.3-179.9-114.4 128.9 11.9 56.4 12.1 71 74 A F E -BC 23 66A 0 -48,-2.6 -48,-2.4 -2,-0.5 2,-0.4 -0.968 7.0-166.5-130.1 145.4 14.1 54.9 14.8 72 75 A T E -BC 22 65A 18 -7,-3.2 -7,-1.5 -2,-0.4 2,-0.5 -0.988 11.0-165.4-139.7 130.5 16.1 56.5 17.6 73 76 A L E -BC 21 64A 0 -52,-3.2 -52,-3.2 -2,-0.4 2,-0.5 -0.951 20.3-166.5-108.9 125.4 18.8 55.2 19.9 74 77 A T E -BC 20 63A 14 -11,-3.3 -11,-1.9 -2,-0.5 2,-0.7 -0.970 16.3-162.2-124.1 127.8 19.4 57.5 22.8 75 78 A I E -BC 19 62A 0 -56,-1.8 -56,-2.1 -2,-0.5 3,-0.5 -0.910 12.3-165.7-105.2 105.5 22.3 57.5 25.2 76 79 A S S S+ 0 0 53 -15,-2.6 2,-0.8 -2,-0.7 -1,-0.2 0.968 76.9 16.8 -53.8 -74.2 21.2 59.6 28.2 77 80 A N S S- 0 0 74 -59,-0.1 -1,-0.3 -62,-0.0 -59,-0.2 -0.727 89.5-141.6-107.5 78.2 24.4 60.3 30.2 78 81 A V - 0 0 0 -61,-2.8 -62,-2.2 -2,-0.8 2,-0.3 0.029 20.5-169.2 -41.3 139.7 27.2 59.5 27.7 79 82 A Q > - 0 0 59 -64,-0.2 3,-1.7 1,-0.1 4,-0.3 -0.860 35.5-104.0-130.0 164.4 30.3 57.8 29.1 80 83 A S G > S+ 0 0 68 -2,-0.3 3,-3.0 1,-0.3 -1,-0.1 0.940 120.3 55.8 -51.9 -55.5 33.8 57.1 27.8 81 84 A E G 3 S+ 0 0 106 1,-0.3 -1,-0.3 -3,-0.1 -3,-0.0 0.528 97.6 68.0 -59.7 -3.0 33.1 53.5 27.2 82 85 A D G < S+ 0 0 2 -3,-1.7 2,-0.5 2,-0.1 -1,-0.3 0.628 70.2 106.3 -90.5 -14.8 30.2 54.7 25.1 83 86 A L < + 0 0 49 -3,-3.0 2,-0.3 -4,-0.3 22,-0.1 -0.537 56.8 95.4 -67.9 117.1 32.5 56.2 22.4 84 87 A A S S- 0 0 7 -2,-0.5 20,-2.7 20,-0.4 2,-0.4 -0.957 78.2 -61.3-177.3-171.5 32.1 53.7 19.6 85 88 A D E -EH 38 103B 41 -47,-2.5 -47,-2.4 -2,-0.3 2,-0.4 -0.803 44.4-157.3 -96.3 138.8 30.4 52.9 16.3 86 89 A Y E -EH 37 102B 0 16,-1.6 16,-1.2 -2,-0.4 2,-0.4 -0.961 10.1-174.5-121.1 134.6 26.6 52.5 16.4 87 90 A F E -E 36 0B 3 -51,-3.0 -51,-3.5 -2,-0.4 2,-0.3 -0.974 13.7-152.7-130.5 143.3 24.6 50.6 13.8 88 91 A a E -E 35 0B 0 -2,-0.4 11,-2.1 -53,-0.2 2,-0.4 -0.814 16.0-164.6-108.2 151.4 20.9 50.0 13.2 89 92 A Q E -EI 34 98B 14 -55,-2.2 -55,-2.1 -2,-0.3 2,-0.4 -0.995 11.0-148.5-138.8 144.7 19.6 46.9 11.5 90 93 A Q E +EI 33 97B 0 7,-2.0 7,-1.2 -2,-0.4 -57,-0.2 -0.925 18.0 170.5-112.6 132.3 16.4 45.9 9.9 91 94 A Y + 0 0 49 -59,-2.4 -58,-0.1 -2,-0.4 3,-0.1 -0.079 48.7 106.4-132.2 35.5 15.2 42.3 9.9 92 95 A S S S- 0 0 62 -60,-0.3 2,-0.3 1,-0.3 4,-0.1 0.947 91.8 -2.2 -75.9 -54.9 11.6 42.8 8.7 93 96 A S S S- 0 0 52 2,-0.2 -1,-0.3 -91,-0.0 4,-0.2 -0.889 93.8 -54.2-135.5 165.3 12.3 41.4 5.2 94 97 A Y S S+ 0 0 122 -2,-0.3 2,-0.1 -3,-0.1 -3,-0.1 -0.603 102.9 47.9 -86.7 150.9 15.2 40.0 3.2 95 98 A P S S- 0 0 21 0, 0.0 -93,-0.4 0, 0.0 -2,-0.2 0.557 88.0-130.3 -81.5 150.1 17.9 40.8 2.5 96 99 A L - 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