==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=31-DEC-2009 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER HYDROLASE 20-JUN-06 2HDU . COMPND 2 MOLECULE: BETA-LACTAMASE; . SOURCE 2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI K12; . AUTHOR K.BABAOGLU,B.K.SHOICHET . 715 3 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 27972.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 514 71.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 8 1.1 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 135 18.9 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 4 0.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 10 1.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 38 5.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 88 12.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 192 26.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 23 3.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 3 4 1 2 3 0 4 2 2 0 2 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 4 7 7 2 0 2 4 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 4 4 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 4 A A 0 0 76 0, 0.0 35,-0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 161.7 22.9 -20.2 34.7 2 5 A P >> - 0 0 56 0, 0.0 4,-2.4 0, 0.0 3,-0.5 -0.342 360.0-113.8 -49.9 144.1 23.8 -19.9 38.4 3 6 A Q H 3> S+ 0 0 146 1,-0.2 4,-3.1 2,-0.2 5,-0.2 0.846 114.1 58.7 -58.3 -35.5 20.8 -18.7 40.2 4 7 A Q H 3> S+ 0 0 105 2,-0.2 4,-2.4 1,-0.2 -1,-0.2 0.909 109.9 43.3 -60.8 -42.9 22.5 -15.4 41.2 5 8 A I H <> S+ 0 0 0 -3,-0.5 4,-2.4 2,-0.2 5,-0.3 0.943 114.0 51.3 -68.6 -46.1 23.1 -14.6 37.5 6 9 A N H X S+ 0 0 57 -4,-2.4 4,-2.8 1,-0.2 -2,-0.2 0.938 112.9 45.6 -51.3 -51.7 19.6 -15.7 36.5 7 10 A D H < S+ 0 0 93 -4,-3.1 -1,-0.2 2,-0.2 -2,-0.2 0.912 113.4 45.6 -67.7 -45.3 18.0 -13.5 39.1 8 11 A I H < S+ 0 0 41 -4,-2.4 5,-0.2 -5,-0.2 -1,-0.2 0.930 116.1 47.2 -66.5 -39.3 19.9 -10.3 38.6 9 12 A V H < 0 0 0 -4,-2.4 4,-3.0 -5,-0.2 5,-0.3 0.981 360.0 360.0 -62.4 -52.9 19.6 -10.5 34.9 10 13 A H < 0 0 74 -4,-2.8 4,-2.1 -5,-0.3 -2,-0.2 0.902 360.0 360.0 -64.5 360.0 15.8 -11.3 34.9 11 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 12 15 A T > 0 0 39 0, 0.0 4,-1.1 0, 0.0 -2,-0.2 0.000 360.0 360.0 360.0 -45.5 16.7 -6.0 35.7 13 16 A I H > + 0 0 0 -4,-3.0 4,-2.7 -5,-0.2 5,-0.2 0.889 360.0 61.5 -80.6 -37.5 15.8 -6.9 32.1 14 17 A T H > S+ 0 0 73 -4,-2.1 4,-1.9 -5,-0.3 -1,-0.2 0.941 109.8 42.1 -52.1 -53.6 12.1 -7.8 32.6 15 18 A P H > S+ 0 0 50 0, 0.0 4,-3.1 0, 0.0 5,-0.3 0.882 111.9 55.8 -63.3 -33.8 11.1 -4.4 33.8 16 19 A L H X S+ 0 0 8 -4,-1.1 4,-2.5 1,-0.2 6,-0.2 0.943 107.8 48.1 -61.7 -46.7 13.3 -2.7 31.2 17 20 A I H X>S+ 0 0 31 -4,-2.7 5,-1.5 1,-0.2 4,-0.9 0.893 114.8 46.6 -61.0 -41.3 11.5 -4.5 28.4 18 21 A E H <5S+ 0 0 148 -4,-1.9 3,-0.4 -5,-0.2 -2,-0.2 0.950 115.4 44.4 -65.1 -51.1 8.1 -3.5 29.9 19 22 A Q H <5S+ 0 0 135 -4,-3.1 -2,-0.2 1,-0.2 -1,-0.2 0.855 122.1 37.0 -63.0 -38.0 9.0 0.1 30.5 20 23 A Q H <5S- 0 0 44 -4,-2.5 -1,-0.2 -5,-0.3 -2,-0.2 0.503 101.6-128.9 -95.7 -4.7 10.7 0.6 27.1 21 24 A K T <5 + 0 0 164 -4,-0.9 -3,-0.2 -3,-0.4 -4,-0.1 0.907 39.2 179.8 53.3 48.0 8.2 -1.6 25.1 22 25 A I < - 0 0 8 -5,-1.5 -1,-0.2 -6,-0.2 3,-0.1 -0.651 22.4-153.4 -80.7 122.8 11.0 -3.6 23.5 23 26 A P S S+ 0 0 28 0, 0.0 19,-2.5 0, 0.0 2,-0.3 0.897 73.2 2.4 -65.1 -37.7 9.8 -6.2 21.2 24 27 A G E +A 41 0A 1 17,-0.3 314,-1.6 19,-0.1 2,-0.3 -0.997 64.5 179.3-153.2 144.3 12.7 -8.6 21.5 25 28 A M E -AB 40 337A 0 15,-2.4 15,-2.3 -2,-0.3 2,-0.4 -1.000 12.0-164.7-150.5 147.1 16.0 -8.8 23.4 26 29 A A E -AB 39 336A 0 310,-2.6 310,-3.0 -2,-0.3 2,-0.4 -0.996 12.6-172.3-130.2 138.0 19.0 -11.0 23.9 27 30 A V E -AB 38 335A 0 11,-2.8 11,-2.4 -2,-0.4 2,-0.4 -0.988 7.3-167.7-130.6 137.7 21.5 -10.6 26.7 28 31 A A E -AB 37 334A 0 306,-2.2 306,-2.4 -2,-0.4 2,-0.5 -0.995 4.6-164.4-117.7 132.9 24.9 -12.3 27.4 29 32 A V E -AB 36 333A 0 7,-2.4 7,-2.1 -2,-0.4 2,-0.6 -0.983 6.8-155.4-110.1 130.4 26.7 -12.1 30.7 30 33 A I E +AB 35 332A 0 302,-2.8 302,-2.1 -2,-0.5 2,-0.4 -0.936 19.8 177.3-101.0 118.3 30.3 -13.1 30.9 31 34 A Y E > S-A 34 0A 52 3,-2.5 3,-2.1 -2,-0.6 300,-0.2 -0.995 70.7 -11.5-131.6 123.0 31.1 -14.2 34.4 32 35 A Q T 3 S- 0 0 133 297,-0.5 299,-0.2 -2,-0.4 -1,-0.2 0.885 130.5 -58.5 48.5 41.6 34.5 -15.5 35.4 33 36 A G T 3 S+ 0 0 27 297,-2.1 -1,-0.3 1,-0.2 298,-0.1 0.299 115.4 111.5 78.4 -8.7 35.1 -15.6 31.6 34 37 A K E < -A 31 0A 128 -3,-2.1 -3,-2.5 296,-0.2 2,-0.3 -0.774 63.7-123.9-100.8 140.9 32.2 -18.0 30.8 35 38 A P E -A 30 0A 42 0, 0.0 2,-0.4 0, 0.0 -5,-0.2 -0.662 20.5-172.2 -90.1 145.3 29.0 -16.9 28.8 36 39 A Y E -A 29 0A 54 -7,-2.1 -7,-2.4 -2,-0.3 2,-0.3 -0.998 15.6-150.9-132.0 128.8 25.4 -17.1 30.0 37 40 A Y E -A 28 0A 60 -2,-0.4 2,-0.3 -9,-0.2 -9,-0.2 -0.817 15.1-180.0-111.5 148.4 22.6 -16.3 27.5 38 41 A F E -A 27 0A 17 -11,-2.4 -11,-2.8 -2,-0.3 2,-0.3 -0.992 4.0-174.6-143.4 138.3 19.1 -14.9 27.9 39 42 A T E +A 26 0A 52 -2,-0.3 2,-0.3 -13,-0.2 -13,-0.2 -0.986 3.5 177.4-136.1 153.7 16.4 -14.1 25.4 40 43 A W E -A 25 0A 40 -15,-2.3 -15,-2.4 -2,-0.3 12,-0.0 -0.995 43.1 -12.4-146.6 145.9 12.9 -12.6 25.4 41 44 A G E S-A 24 0A 37 -2,-0.3 10,-2.3 -17,-0.2 2,-0.4 -0.195 86.5 -19.3 75.0-153.3 10.3 -11.7 23.0 42 45 A Y E -I 50 0B 109 -19,-2.5 3,-0.2 8,-0.3 296,-0.2 -0.867 19.2-174.1-118.9 131.1 10.1 -11.4 19.2 43 46 A A E S+ 0 0 8 6,-2.3 155,-2.7 -2,-0.4 2,-0.4 0.738 100.4 19.7 -77.2 -33.9 12.6 -11.0 16.4 44 47 A D E > -I 49 0B 18 5,-1.7 5,-2.0 153,-0.2 4,-0.4 -0.922 68.4-169.1-142.6 108.4 9.7 -10.6 14.0 45 48 A I T > 5S+ 0 0 84 -2,-0.4 3,-1.1 -3,-0.2 -1,-0.1 0.954 87.7 48.5 -66.7 -48.1 6.3 -9.6 15.5 46 49 A A T 3 5S+ 0 0 89 1,-0.3 -1,-0.2 2,-0.1 -2,-0.0 0.876 118.4 39.4 -60.3 -43.9 4.2 -10.3 12.3 47 50 A K T 3 5S- 0 0 147 2,-0.1 -1,-0.3 0, 0.0 -2,-0.2 0.395 106.7-127.4 -88.5 1.2 5.7 -13.7 11.7 48 51 A K T < 5 + 0 0 159 -3,-1.1 -3,-0.2 -4,-0.4 -2,-0.1 0.803 49.7 162.4 58.7 35.4 5.7 -14.5 15.5 49 52 A Q E < -I 44 0B 95 -5,-2.0 -6,-2.3 1,-0.0 -5,-1.7 -0.762 30.4-136.1 -89.7 125.4 9.4 -15.5 15.4 50 53 A P E -I 42 0B 70 0, 0.0 2,-0.4 0, 0.0 -8,-0.3 -0.390 20.3-103.8 -76.1 158.7 11.1 -15.5 18.8 51 54 A V + 0 0 4 -10,-2.3 2,-0.2 -26,-0.1 -12,-0.1 -0.654 49.9 174.2 -72.0 133.8 14.5 -14.1 19.7 52 55 A T > - 0 0 44 -2,-0.4 3,-1.3 143,-0.1 172,-0.3 -0.747 51.1 -91.9-130.2 177.2 16.9 -17.0 20.0 53 56 A Q T 3 S+ 0 0 71 1,-0.2 172,-1.0 -2,-0.2 171,-0.1 0.601 127.2 53.6 -67.9 -11.1 20.7 -17.3 20.5 54 57 A Q T 3 S+ 0 0 96 169,-0.1 -1,-0.2 170,-0.1 2,-0.2 0.391 79.6 119.8-100.2 -5.6 20.9 -17.4 16.7 55 58 A T < - 0 0 4 -3,-1.3 2,-0.5 141,-0.1 169,-0.5 -0.447 58.7-135.8 -65.2 134.1 19.0 -14.2 16.0 56 59 A L B -j 196 0C 1 139,-3.0 141,-2.3 136,-0.2 2,-0.3 -0.819 21.6-171.6 -91.8 130.3 21.0 -11.4 14.2 57 60 A F E -K 222 0D 1 165,-2.7 165,-2.1 -2,-0.5 2,-0.5 -0.899 30.7-110.8-115.8 148.6 20.6 -7.9 15.5 58 61 A E E -K 221 0D 3 -2,-0.3 163,-0.2 163,-0.2 279,-0.2 -0.684 25.6-161.3 -72.5 121.3 21.9 -4.7 13.9 59 62 A L > - 0 0 0 161,-2.7 3,-2.1 -2,-0.5 262,-0.2 0.612 16.7-172.5 -76.2 -18.3 24.8 -3.5 16.2 60 63 A G G > S- 0 0 0 160,-0.5 3,-2.6 158,-0.5 160,-0.4 -0.264 70.4 -1.0 53.4-136.3 24.7 0.0 14.9 61 64 A S G > S+ 0 0 14 254,-0.5 3,-1.9 1,-0.3 4,-0.3 0.592 116.5 80.9 -65.5 -9.4 27.6 2.0 16.3 62 65 A V G X> S+ 0 0 0 -3,-2.1 3,-1.9 1,-0.3 4,-0.6 0.800 79.2 76.0 -62.6 -21.6 28.9 -1.0 18.4 63 66 A S H X> S+ 0 0 0 -3,-2.6 4,-2.0 1,-0.3 3,-0.6 0.768 77.2 73.4 -50.3 -32.8 30.3 -1.8 14.9 64 67 A K H <> S+ 0 0 0 -3,-1.9 4,-2.7 153,-0.4 -1,-0.3 0.747 86.5 62.0 -60.1 -25.5 32.9 0.9 15.6 65 68 A T H <> S+ 0 0 0 -3,-1.9 4,-2.1 -4,-0.3 -1,-0.2 0.885 105.2 46.6 -65.6 -40.6 34.6 -1.5 18.1 66 69 A F H S+ 0 0 2 -4,-1.9 5,-2.3 2,-0.2 6,-0.9 0.898 110.7 48.3 -63.3 -39.3 46.6 -3.1 10.5 75 78 A I H ><5S+ 0 0 10 -4,-2.2 3,-2.0 1,-0.2 -2,-0.2 0.948 110.6 50.6 -60.4 -50.1 48.9 -1.0 12.8 76 79 A A H 3<5S+ 0 0 16 -4,-2.6 -2,-0.2 1,-0.3 -1,-0.2 0.837 107.1 54.8 -60.0 -33.5 50.2 -4.2 14.3 77 80 A R T 3<5S- 0 0 107 -4,-2.1 -1,-0.3 -5,-0.2 -2,-0.2 0.556 115.1-118.8 -71.8 -8.5 50.9 -5.6 10.8 78 81 A G T < 5S+ 0 0 57 -3,-2.0 -3,-0.2 -4,-0.3 -2,-0.1 0.644 82.8 119.3 71.6 19.3 53.0 -2.5 10.1 79 82 A E S - 0 0 68 -2,-0.3 3,-1.8 4,-0.1 22,-0.3 -0.994 20.7-133.5-129.0 142.9 50.6 4.1 11.6 82 85 A L T 3 S+ 0 0 9 -2,-0.3 22,-2.4 1,-0.3 23,-0.3 0.723 108.9 60.4 -67.6 -21.2 48.2 6.3 13.6 83 86 A S T 3 S+ 0 0 22 576,-0.3 -1,-0.3 20,-0.2 575,-0.2 0.496 82.3 112.0 -79.5 -6.4 50.7 9.2 13.2 84 87 A D S < S- 0 0 3 -3,-1.8 19,-2.2 1,-0.1 20,-0.2 -0.436 77.1-111.3 -65.8 137.6 50.4 8.9 9.4 85 88 A P B >> -M 102 0E 58 0, 0.0 3,-1.6 0, 0.0 4,-0.5 -0.415 21.8-122.4 -60.5 143.6 48.7 11.9 7.6 86 89 A T H >> S+ 0 0 0 15,-2.7 3,-1.5 12,-0.3 4,-0.9 0.870 111.5 63.8 -53.5 -39.3 45.3 11.0 6.1 87 90 A T H 34 S+ 0 0 44 14,-0.3 -1,-0.3 1,-0.3 6,-0.1 0.673 84.9 73.2 -66.2 -18.4 46.7 12.1 2.7 88 91 A K H <4 S+ 0 0 119 -3,-1.6 -1,-0.3 1,-0.2 -2,-0.2 0.872 110.8 30.6 -58.4 -36.0 49.3 9.3 2.8 89 92 A Y H << S+ 0 0 25 -3,-1.5 -2,-0.2 -4,-0.5 -1,-0.2 0.537 130.8 35.7-103.4 -9.7 46.3 6.9 2.1 90 93 A W >< + 0 0 26 -4,-0.9 3,-2.1 3,-0.1 -1,-0.2 -0.659 64.9 173.6-139.3 77.2 44.1 9.3 0.1 91 94 A P T 3 S+ 0 0 78 0, 0.0 -4,-0.1 0, 0.0 -1,-0.1 0.738 74.0 69.2 -62.9 -24.1 46.5 11.4 -1.9 92 95 A E T 3 S+ 0 0 104 1,-0.2 2,-2.2 -3,-0.1 3,-0.2 0.582 75.1 92.9 -65.7 -13.9 43.5 13.0 -3.8 93 96 A L < + 0 0 2 -3,-2.1 -1,-0.2 1,-0.2 -3,-0.1 -0.503 48.5 147.3 -80.1 74.5 42.7 14.7 -0.5 94 97 A T + 0 0 105 -2,-2.2 -1,-0.2 3,-0.0 5,-0.1 0.570 19.2 122.2 -90.9 -10.2 44.8 17.7 -1.5 95 98 A A > - 0 0 21 -3,-0.2 3,-2.0 1,-0.1 4,-0.2 -0.263 67.8-126.0 -62.0 139.6 42.8 20.4 0.3 96 99 A K G > S+ 0 0 172 1,-0.3 3,-1.6 2,-0.2 -1,-0.1 0.708 102.2 72.0 -66.9 -21.5 45.0 22.3 2.8 97 100 A Q G 3 S+ 0 0 28 1,-0.3 -1,-0.3 36,-0.2 -2,-0.1 0.697 89.9 64.6 -65.2 -18.2 42.7 21.8 5.8 98 101 A W G X S+ 0 0 1 -3,-2.0 3,-2.2 2,-0.1 2,-0.4 0.606 72.9 110.2 -80.1 -14.2 43.9 18.2 5.7 99 102 A N T < S+ 0 0 117 -3,-1.6 3,-0.1 1,-0.3 -5,-0.0 -0.483 88.4 16.3 -61.5 120.1 47.4 19.2 6.5 100 103 A G T 3 S+ 0 0 51 -2,-0.4 2,-0.7 1,-0.2 -1,-0.3 0.389 91.8 122.5 98.5 -3.9 48.0 17.9 10.0 101 104 A I < - 0 0 0 -3,-2.2 -15,-2.7 40,-0.1 -14,-0.3 -0.851 43.1-170.8 -97.2 113.1 45.0 15.5 10.2 102 105 A T B > -M 85 0E 21 -2,-0.7 4,-2.0 -17,-0.3 5,-0.2 -0.475 40.3-101.9 -97.5 168.9 46.3 12.1 11.0 103 106 A L H > S+ 0 0 0 -19,-2.2 4,-2.6 -22,-0.3 5,-0.2 0.870 122.1 60.7 -56.1 -37.4 44.7 8.6 11.1 104 107 A L H > S+ 0 0 9 -22,-2.4 4,-1.6 -20,-0.2 6,-0.3 0.941 103.1 50.4 -52.6 -49.2 44.6 9.0 14.9 105 108 A H H 4>S+ 0 0 22 -23,-0.3 5,-2.0 1,-0.2 6,-0.7 0.926 112.0 46.3 -60.2 -44.0 42.4 12.1 14.5 106 109 A L H ><5S+ 0 0 0 -4,-2.0 3,-0.9 1,-0.2 -1,-0.2 0.926 112.7 50.1 -60.2 -46.9 39.9 10.2 12.2 107 110 A A H 3<5S+ 0 0 0 -4,-2.6 160,-0.6 1,-0.2 -1,-0.2 0.769 118.0 38.6 -64.2 -26.1 39.8 7.2 14.5 108 111 A T T 3<5S- 0 0 0 -4,-1.6 -1,-0.2 -3,-0.3 -2,-0.2 0.179 108.8-111.4-118.2 11.8 39.0 9.2 17.6 109 112 A Y T < 5S+ 0 0 4 -3,-0.9 37,-2.2 -4,-0.5 3,-0.2 0.734 85.3 115.6 67.9 27.8 36.7 12.0 16.3 110 113 A T < + 0 0 0 -5,-2.0 32,-2.9 -6,-0.3 33,-0.5 0.154 28.1 111.1-115.8 19.5 39.3 14.7 16.8 111 114 A A S S- 0 0 1 -6,-0.7 -1,-0.2 30,-0.2 -5,-0.1 0.679 100.6 -84.5 -68.7 -18.2 39.9 15.8 13.2 112 115 A G S S- 0 0 0 32,-0.4 27,-1.5 -3,-0.2 26,-1.5 0.655 91.1 -30.4 119.5 27.4 38.3 19.2 13.9 113 116 A G + 0 0 16 31,-0.2 23,-0.1 -4,-0.2 3,-0.1 0.663 67.0 173.1 110.5 25.4 34.5 18.8 13.5 114 117 A L - 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