==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=31-DEC-2009 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER STRUCTURAL GENOMICS, UNKNOWN FUNCTION 26-JUN-06 2HGA . COMPND 2 MOLECULE: CONSERVED PROTEIN MTH1368; . SOURCE 2 ORGANISM_SCIENTIFIC: METHANOTHERMOBACTER . AUTHOR G.LIU,Y.LIN,D.PARISH,Y.SHEN,D.SUKUMARAN,A.YEE,A.SEMESI, . 103 1 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 7624.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 66 64.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 2 1.9 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 24 23.3 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 2 1.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 1 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 20 19.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 9 8.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 8 7.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 2 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 23 A Q 0 0 175 0, 0.0 2,-0.4 0, 0.0 86,-0.3 0.000 360.0 360.0 360.0 97.1 -2.3 -10.5 -1.9 2 24 A P + 0 0 66 0, 0.0 34,-0.1 0, 0.0 3,-0.1 -0.673 360.0 157.4 -72.7 128.3 -2.4 -7.4 0.3 3 25 A D - 0 0 147 1,-0.6 33,-0.2 -2,-0.4 2,-0.1 -0.004 62.8 -43.7-147.9 29.2 1.2 -6.5 1.1 4 26 A G - 0 0 10 31,-2.7 -1,-0.6 29,-0.1 2,-0.5 -0.209 61.2 -95.5 115.6 148.9 1.1 -2.8 1.9 5 27 A V E -A 26 0A 1 29,-1.1 21,-2.8 21,-1.0 2,-0.3 -0.873 38.2-158.6-102.6 129.7 -0.6 0.3 0.4 6 28 A Q E -AB 25 83A 49 77,-3.6 77,-2.4 -2,-0.5 2,-0.6 -0.793 20.6-114.6-110.7 149.7 1.5 2.4 -2.0 7 29 A I E - B 0 82A 1 17,-3.5 16,-1.8 -2,-0.3 75,-0.2 -0.724 29.3-179.6 -82.3 117.8 1.1 6.0 -3.0 8 30 A D E - 0 0 71 73,-3.0 2,-0.3 -2,-0.6 -1,-0.2 0.969 61.2 -21.0 -79.1 -65.1 0.3 6.2 -6.7 9 31 A S E - B 0 81A 38 72,-1.0 72,-1.9 13,-0.1 2,-0.6 -0.980 52.6-130.7-150.1 156.5 0.1 10.0 -7.3 10 32 A V E - B 0 80A 36 -2,-0.3 70,-0.2 70,-0.2 69,-0.0 -0.940 24.7-174.2-116.2 113.7 -0.4 13.2 -5.4 11 33 A V - 0 0 77 68,-0.9 3,-0.4 -2,-0.6 6,-0.1 -0.769 33.4 -93.5-105.7 153.5 -3.0 15.7 -6.7 12 34 A P S S+ 0 0 120 0, 0.0 -1,-0.1 0, 0.0 67,-0.0 -0.244 99.7 64.2 -62.7 151.0 -3.7 19.2 -5.4 13 35 A G S S+ 0 0 49 1,-0.5 55,-0.8 56,-0.0 56,-0.1 0.403 85.9 95.0 109.7 3.5 -6.4 19.7 -2.8 14 36 A S S > S- 0 0 12 -3,-0.4 3,-1.2 53,-0.1 -1,-0.5 -0.930 74.7-134.2-125.3 148.0 -4.6 17.7 -0.2 15 37 A P G > S+ 0 0 49 0, 0.0 3,-0.9 0, 0.0 51,-0.1 0.773 107.7 65.6 -67.3 -23.4 -2.3 18.7 2.7 16 38 A A G >> S+ 0 0 0 62,-0.5 3,-1.5 49,-0.2 4,-1.5 0.563 70.8 99.5 -75.3 -7.9 -0.0 15.9 1.5 17 39 A S G <4 S+ 0 0 43 -3,-1.2 -1,-0.2 1,-0.3 -7,-0.1 0.776 102.0 17.0 -53.3 -31.2 0.6 17.7 -1.7 18 40 A K G <4 S+ 0 0 197 -3,-0.9 -1,-0.3 -4,-0.1 -2,-0.2 0.113 119.1 68.5-129.0 21.2 4.0 19.0 -0.4 19 41 A V T <4 S+ 0 0 63 -3,-1.5 2,-0.2 1,-0.1 -2,-0.2 0.827 102.8 22.2-103.7 -51.0 4.5 16.6 2.5 20 42 A L S < S- 0 0 16 -4,-1.5 -1,-0.1 42,-0.0 -10,-0.0 -0.511 76.1-112.6-112.3-179.8 5.1 13.2 0.9 21 43 A T > - 0 0 81 -2,-0.2 3,-1.2 -3,-0.1 -14,-0.1 -0.915 27.2-114.4-116.5 138.3 6.3 11.9 -2.4 22 44 A P T 3 S+ 0 0 68 0, 0.0 -14,-0.2 0, 0.0 -13,-0.1 -0.476 101.1 39.2 -64.3 141.9 4.3 10.0 -5.0 23 45 A G T 3 S+ 0 0 39 -16,-1.8 -15,-0.1 1,-0.3 2,-0.1 0.316 86.6 127.5 99.5 -7.8 5.6 6.4 -5.5 24 46 A L < - 0 0 22 -3,-1.2 -17,-3.5 -18,-0.1 2,-0.5 -0.371 56.9-123.3 -81.8 163.3 6.3 5.9 -1.8 25 47 A V E -A 6 0A 55 -19,-0.3 33,-1.1 -3,-0.1 2,-0.7 -0.924 12.1-138.1-113.3 126.7 5.0 2.9 0.2 26 48 A I E +AC 5 57A 1 -21,-2.8 -21,-1.0 -2,-0.5 31,-0.2 -0.730 23.3 176.1 -84.6 113.0 3.0 3.3 3.3 27 49 A E E - 0 0 92 29,-2.1 7,-0.6 -2,-0.7 2,-0.3 0.911 61.3 -19.3 -83.9 -46.5 4.2 0.8 5.9 28 50 A S E -DC 33 56A 35 28,-1.7 28,-2.7 5,-0.2 -1,-0.3 -0.990 51.8-136.5-162.8 152.1 2.1 1.7 8.9 29 51 A I E > -DC 32 55A 5 3,-2.9 3,-1.7 -2,-0.3 2,-0.5 -0.549 62.0 -55.6-103.3 172.5 -0.1 4.5 10.4 30 52 A N T 3 S- 0 0 67 24,-0.5 -1,-0.1 1,-0.3 25,-0.1 -0.313 122.0 -23.7 -56.1 101.9 -0.1 5.6 14.0 31 53 A G T 3 S+ 0 0 83 -2,-0.5 -1,-0.3 1,-0.1 -3,-0.0 0.478 129.7 88.9 75.0 1.8 -0.8 2.4 15.9 32 54 A M E < -D 29 0A 52 -3,-1.7 -3,-2.9 2,-0.0 2,-0.3 -0.852 64.9-155.5-138.1 96.7 -2.4 1.0 12.8 33 55 A P E -D 28 0A 83 0, 0.0 2,-0.8 0, 0.0 -5,-0.2 -0.551 8.4-149.1 -73.9 133.9 -0.3 -0.9 10.2 34 56 A T + 0 0 0 -7,-0.6 -29,-1.1 -2,-0.3 -31,-0.1 -0.742 42.8 140.5-110.8 83.8 -1.7 -0.9 6.7 35 57 A S S S+ 0 0 60 -2,-0.8 -31,-2.7 -31,-0.2 2,-0.2 0.567 71.2 42.0 -91.9 -13.5 -0.7 -4.1 5.0 36 58 A N S > S- 0 0 55 -3,-0.2 4,-0.9 -33,-0.2 -31,-0.1 -0.747 81.8-117.6-128.1 173.8 -4.1 -4.4 3.3 37 59 A L H > S+ 0 0 82 -2,-0.2 4,-1.8 2,-0.2 5,-0.1 0.872 114.4 50.1 -78.7 -39.2 -6.7 -2.2 1.6 38 60 A T H > S+ 0 0 114 2,-0.2 4,-3.0 1,-0.2 5,-0.2 0.903 108.4 51.4 -67.1 -42.5 -9.4 -3.0 4.3 39 61 A T H > S+ 0 0 53 1,-0.2 4,-1.8 2,-0.2 -1,-0.2 0.842 107.3 54.9 -65.6 -32.4 -7.0 -2.2 7.2 40 62 A Y H X S+ 0 0 32 -4,-0.9 4,-2.4 2,-0.2 -1,-0.2 0.926 112.0 43.2 -62.1 -44.5 -6.2 1.1 5.5 41 63 A S H X S+ 0 0 45 -4,-1.8 4,-2.8 2,-0.2 -2,-0.2 0.907 109.9 56.2 -68.1 -41.7 -10.0 1.9 5.4 42 64 A A H < S+ 0 0 58 -4,-3.0 4,-0.2 1,-0.2 -1,-0.2 0.844 112.2 43.4 -60.7 -34.7 -10.5 0.7 9.0 43 65 A A H >X S+ 0 0 3 -4,-1.8 3,-1.5 -5,-0.2 4,-0.6 0.927 112.8 52.0 -73.1 -45.6 -7.8 3.1 10.1 44 66 A L H >< S+ 0 0 21 -4,-2.4 3,-1.3 1,-0.3 -2,-0.2 0.853 99.4 63.6 -60.2 -38.2 -9.2 5.9 7.9 45 67 A K T 3< S+ 0 0 131 -4,-2.8 -1,-0.3 1,-0.3 -2,-0.2 0.639 104.1 48.6 -65.4 -14.4 -12.8 5.5 9.3 46 68 A T T <4 S+ 0 0 88 -3,-1.5 -1,-0.3 -4,-0.2 2,-0.3 0.586 94.3 90.9 -99.3 -13.1 -11.5 6.5 12.7 47 69 A I << - 0 0 10 -3,-1.3 2,-0.2 -4,-0.6 6,-0.0 -0.600 65.3-145.2 -88.7 144.2 -9.6 9.6 11.6 48 70 A S > - 0 0 65 -2,-0.3 3,-1.3 27,-0.1 2,-0.6 -0.546 29.3 -85.7-104.1 171.0 -11.3 13.0 11.5 49 71 A V T 3 S+ 0 0 64 1,-0.3 3,-0.1 -2,-0.2 18,-0.1 -0.656 116.3 18.7 -81.4 118.0 -11.0 16.0 9.1 50 72 A G T 3 S+ 0 0 40 -2,-0.6 -1,-0.3 1,-0.4 2,-0.2 0.368 96.3 128.7 102.6 -1.6 -8.1 18.2 10.2 51 73 A E < - 0 0 87 -3,-1.3 15,-2.5 16,-0.1 2,-0.5 -0.590 53.6-134.1 -87.6 149.1 -6.6 15.5 12.3 52 74 A V E - E 0 65A 84 13,-0.2 13,-0.3 -2,-0.2 2,-0.2 -0.915 21.1-169.6-111.1 124.9 -2.9 14.6 12.0 53 75 A I E - E 0 64A 2 11,-3.1 11,-2.5 -2,-0.5 2,-0.6 -0.677 23.5-117.5-110.1 164.3 -1.8 11.0 11.7 54 76 A N E - E 0 63A 51 -2,-0.2 2,-0.6 9,-0.2 -24,-0.5 -0.897 30.0-174.3-107.5 114.9 1.7 9.3 11.9 55 77 A I E -CE 29 62A 4 7,-3.0 7,-3.3 -2,-0.6 2,-0.5 -0.932 9.0-160.6-117.5 115.2 2.7 7.5 8.7 56 78 A T E +CE 28 61A 33 -28,-2.7 -29,-2.1 -2,-0.6 -28,-1.7 -0.820 21.5 168.9 -93.0 125.4 5.9 5.5 8.6 57 79 A T E > -CE 26 60A 8 3,-2.6 3,-1.7 -2,-0.5 -31,-0.2 -0.722 52.9 -94.6-124.2 177.5 7.1 4.7 5.1 58 80 A D T 3 S+ 0 0 115 -33,-1.1 3,-0.1 1,-0.3 -32,-0.1 0.852 127.6 54.5 -57.9 -33.7 10.3 3.4 3.4 59 81 A Q T 3 S- 0 0 113 1,-0.3 -1,-0.3 -34,-0.2 2,-0.1 0.168 127.8 -87.7 -90.7 17.3 11.3 7.0 3.0 60 82 A G E < -E 57 0A 33 -3,-1.7 -3,-2.6 2,-0.0 2,-0.4 -0.413 57.3 -49.6 107.7 178.4 10.9 7.6 6.7 61 83 A T E +E 56 0A 72 -5,-0.3 2,-0.3 -2,-0.1 -5,-0.3 -0.787 54.4 179.5 -94.6 131.6 8.1 8.5 9.1 62 84 A F E -E 55 0A 61 -7,-3.3 -7,-3.0 -2,-0.4 2,-0.5 -0.879 27.2-124.8-125.2 159.7 6.0 11.6 8.2 63 85 A H E -E 54 0A 131 -2,-0.3 2,-0.4 -9,-0.2 -9,-0.2 -0.935 30.4-165.0-106.6 124.3 3.1 13.3 9.9 64 86 A L E -E 53 0A 1 -11,-2.5 -11,-3.1 -2,-0.5 2,-0.4 -0.901 13.0-146.5-116.7 135.9 0.1 13.8 7.6 65 87 A K E -E 52 0A 110 -2,-0.4 -49,-0.2 -13,-0.3 -13,-0.2 -0.819 32.6-109.3 -95.2 135.8 -3.0 15.9 8.0 66 88 A T - 0 0 3 -15,-2.5 -16,-0.3 -2,-0.4 12,-0.2 -0.357 40.4-109.2 -65.1 143.5 -6.2 14.6 6.6 67 89 A G - 0 0 1 1,-0.1 10,-0.9 -18,-0.1 9,-0.2 0.015 18.9-110.8 -72.9-177.6 -7.5 16.3 3.5 68 90 A R B -f 77 0B 207 -55,-0.8 -1,-0.1 8,-0.1 -54,-0.1 0.275 55.2-104.6-103.9 8.4 -10.4 18.6 3.1 69 91 A N - 0 0 32 8,-0.5 2,-0.4 -56,-0.1 -55,-0.1 0.972 35.4-152.9 66.6 88.7 -12.6 16.2 1.0 70 92 A P S S- 0 0 59 0, 0.0 2,-1.8 0, 0.0 -1,-0.1 -0.799 72.6 -13.0 -91.1 134.1 -12.7 17.2 -2.7 71 93 A N S S+ 0 0 164 -2,-0.4 2,-0.4 2,-0.0 -2,-0.0 -0.092 131.7 58.8 72.4 -38.5 -15.8 16.3 -4.8 72 94 A N S S- 0 0 82 -2,-1.8 2,-0.6 5,-0.0 5,-0.0 -0.927 77.2-132.1-126.5 145.7 -17.0 14.0 -2.1 73 95 A S + 0 0 122 -2,-0.4 2,-0.2 2,-0.0 -4,-0.1 -0.866 50.3 119.9 -99.6 119.7 -17.9 14.5 1.6 74 96 A S - 0 0 75 -2,-0.6 2,-0.5 -6,-0.0 3,-0.2 -0.832 64.0 -76.1-152.9-168.9 -16.4 12.0 4.0 75 97 A R S S- 0 0 152 1,-0.3 -28,-0.2 -2,-0.2 -27,-0.1 -0.924 101.9 -2.4-105.9 127.9 -14.1 11.8 7.1 76 98 A A - 0 0 5 -2,-0.5 2,-0.8 -9,-0.2 -1,-0.3 0.974 69.2-163.6 61.4 90.0 -10.3 12.2 6.5 77 99 A Y B -f 68 0B 85 -10,-0.9 -8,-0.5 -3,-0.2 -1,-0.2 -0.822 6.1-165.5-111.6 96.0 -9.8 12.5 2.8 78 100 A M - 0 0 10 -2,-0.8 -62,-0.5 -12,-0.2 -1,-0.2 0.878 18.1-149.0 -47.0 -54.2 -6.1 11.9 2.0 79 101 A G + 0 0 16 1,-0.3 -68,-0.9 -65,-0.1 2,-0.4 0.403 59.4 103.4 99.3 -2.2 -6.3 13.3 -1.5 80 102 A I E S-B 10 0A 11 -70,-0.2 2,-0.4 -72,-0.0 -1,-0.3 -0.911 78.5-110.9-114.6 140.6 -3.7 11.0 -3.0 81 103 A R E +B 9 0A 158 -72,-1.9 -73,-3.0 -2,-0.4 -72,-1.0 -0.558 46.3 178.6 -69.5 125.4 -4.3 8.0 -5.2 82 104 A T E +B 7 0A 36 -2,-0.4 2,-0.3 -75,-0.2 -75,-0.2 -0.982 10.7 164.7-135.5 142.0 -3.3 5.0 -3.2 83 105 A S E -B 6 0A 55 -77,-2.4 -77,-3.6 -2,-0.4 -2,-0.0 -0.987 46.5-118.9-153.4 157.4 -3.4 1.3 -3.9 84 106 A N - 0 0 27 -2,-0.3 -81,-0.3 -79,-0.3 -77,-0.1 0.560 52.5-129.7 -74.7 -8.8 -2.0 -2.0 -2.6 85 107 A H - 0 0 127 -79,-0.2 3,-0.1 1,-0.1 -2,-0.0 0.988 22.1-130.6 57.9 76.0 -0.3 -2.4 -6.0 86 108 A L - 0 0 105 1,-0.1 2,-0.3 -84,-0.0 -1,-0.1 -0.251 24.1-101.3 -59.3 141.6 -1.3 -5.9 -6.9 87 109 A R - 0 0 190 -86,-0.3 -1,-0.1 1,-0.1 2,-0.1 -0.493 43.8-113.8 -67.9 121.1 1.4 -8.2 -8.1 88 110 A V + 0 0 117 -2,-0.3 2,-0.3 -3,-0.1 -1,-0.1 -0.355 56.3 135.9 -66.0 129.1 1.2 -8.4 -11.9 89 111 A R - 0 0 199 -2,-0.1 2,-0.8 2,-0.0 -3,-0.0 -0.936 56.9 -98.6-157.0 175.3 0.3 -11.8 -13.3 90 112 A D + 0 0 144 -2,-0.3 2,-1.1 2,-0.1 -2,-0.0 -0.738 42.0 159.4-111.3 82.3 -2.0 -13.3 -15.9 91 113 A S - 0 0 115 -2,-0.8 -2,-0.0 2,-0.0 -1,-0.0 -0.804 37.5-150.4 -93.5 86.0 -5.2 -14.5 -14.4 92 114 A V - 0 0 116 -2,-1.1 2,-0.6 1,-0.1 -2,-0.1 -0.274 16.9-155.6 -67.9 143.0 -7.1 -14.4 -17.6 93 115 A A - 0 0 100 -2,-0.0 2,-0.1 3,-0.0 -1,-0.1 -0.807 21.0-167.2-116.6 85.4 -10.8 -13.9 -17.8 94 116 A S - 0 0 91 -2,-0.6 2,-0.3 1,-0.1 0, 0.0 -0.382 29.9 -99.7 -74.4 150.1 -11.9 -15.4 -21.1 95 117 A V - 0 0 147 1,-0.2 -1,-0.1 -2,-0.1 3,-0.1 -0.563 33.3-171.8 -70.2 126.4 -15.3 -14.8 -22.6 96 118 A L - 0 0 179 -2,-0.3 2,-0.2 1,-0.2 -1,-0.2 0.885 49.3 -84.6 -86.6 -43.3 -17.6 -17.7 -21.8 97 119 A G - 0 0 70 2,-0.0 2,-0.5 0, 0.0 -1,-0.2 -0.502 28.5-119.0 141.5 146.4 -20.5 -16.8 -23.9 98 120 A D - 0 0 139 -2,-0.2 2,-0.6 -3,-0.1 -3,-0.0 -0.952 20.1-142.8-119.8 117.7 -23.6 -14.6 -23.8 99 121 A T - 0 0 131 -2,-0.5 -2,-0.0 1,-0.0 0, 0.0 -0.676 14.9-163.4 -82.6 118.1 -27.0 -16.2 -24.1 100 122 A L - 0 0 143 -2,-0.6 2,-0.1 2,-0.0 -2,-0.0 -0.884 6.5-151.5-107.2 106.3 -29.4 -13.9 -26.1 101 123 A P - 0 0 122 0, 0.0 2,-0.5 0, 0.0 0, 0.0 -0.403 7.1-144.6 -70.9 150.8 -33.1 -14.7 -25.7 102 124 A F 0 0 198 -2,-0.1 -2,-0.0 0, 0.0 0, 0.0 -0.972 360.0 360.0-125.1 117.3 -35.4 -13.9 -28.6 103 125 A A 0 0 162 -2,-0.5 0, 0.0 0, 0.0 0, 0.0 -0.746 360.0 360.0 -88.5 360.0 -39.0 -12.8 -27.9