==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=20-JUL-2011 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER SIGNALING PROTEIN/CYTOKINE 14-MAY-09 3HH2 . COMPND 2 MOLECULE: GROWTH/DIFFERENTIATION FACTOR 8; . SOURCE 2 ORGANISM_SCIENTIFIC: MUS MUSCULUS; . AUTHOR J.N.CASH,T.B.THOMPSON . 771 9 45 35 10 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 40019.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 440 57.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 14 1.8 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 174 22.6 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 2 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 13 1.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 1 0.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 68 8.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 49 6.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 92 11.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 12 1.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 4 0 0 0 0 2 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 4 10 12 4 4 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 6 11 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 A D 0 0 54 0, 0.0 3,-0.0 0, 0.0 531,-0.0 0.000 360.0 360.0 360.0 20.8 -11.4 -10.7 26.3 2 2 A F + 0 0 78 505,-0.0 2,-0.3 2,-0.0 504,-0.1 0.502 360.0 32.8 -99.5 -10.4 -14.8 -12.3 26.8 3 3 A G S S- 0 0 15 503,-0.1 2,-0.4 101,-0.1 104,-0.1 -0.868 77.3-105.3-140.0 174.2 -16.6 -9.3 28.3 4 4 A L - 0 0 16 102,-0.4 13,-2.8 11,-0.3 2,-0.4 -0.885 22.9-158.9-103.7 136.1 -16.8 -5.6 28.4 5 5 A D B +a 17 0A 105 -2,-0.4 2,-0.3 11,-0.2 13,-0.2 -0.932 17.8 179.1-109.3 137.2 -15.5 -3.4 31.3 6 6 A a - 0 0 16 11,-3.5 2,-0.3 -2,-0.4 10,-0.0 -0.916 16.1-152.4-134.6 157.4 -16.9 0.1 31.8 7 7 A D > - 0 0 75 -2,-0.3 3,-2.8 11,-0.2 11,-0.0 -0.760 47.3 -85.7-120.7 176.6 -16.6 3.0 34.2 8 8 A E T 3 S+ 0 0 138 1,-0.3 10,-0.0 -2,-0.3 -2,-0.0 0.691 127.6 58.2 -52.6 -19.7 -19.0 5.8 35.2 9 9 A H T 3 S+ 0 0 150 2,-0.0 -1,-0.3 0, 0.0 2,-0.2 0.263 80.9 111.2 -99.7 12.3 -17.8 7.6 32.1 10 10 A S < - 0 0 44 -3,-2.8 -4,-0.1 1,-0.1 0, 0.0 -0.535 53.4-158.6 -78.8 150.9 -18.8 4.9 29.6 11 11 A T + 0 0 148 -2,-0.2 2,-0.6 0, 0.0 -1,-0.1 -0.138 46.6 141.1-114.9 34.0 -21.7 5.6 27.2 12 12 A E - 0 0 40 1,-0.2 4,-0.1 2,-0.1 -2,-0.1 -0.662 31.7-176.6 -80.4 118.0 -22.0 1.8 26.8 13 13 A S + 0 0 76 -2,-0.6 33,-2.9 32,-0.1 -1,-0.2 0.800 57.9 87.4 -81.1 -27.9 -25.6 0.7 26.5 14 14 A R B S-c 46 0B 31 31,-0.3 33,-0.2 1,-0.2 30,-0.1 -0.191 99.8 -68.3 -77.9 163.3 -24.8 -3.1 26.4 15 15 A b S S+ 0 0 1 31,-1.8 -11,-0.3 30,-0.2 2,-0.3 -0.271 71.1 164.0 -50.0 121.7 -24.2 -5.5 29.2 16 16 A a E - B 0 44A 1 28,-2.1 28,-2.2 -3,-0.1 2,-0.5 -0.951 40.7-112.6-145.5 155.1 -21.0 -4.4 30.9 17 17 A R E -aB 5 43A 53 -13,-2.8 -11,-3.5 -2,-0.3 26,-0.2 -0.838 34.9-172.9 -97.2 129.6 -19.0 -4.8 34.1 18 18 A Y E - B 0 42A 32 24,-3.2 24,-2.4 -2,-0.5 -11,-0.2 -0.850 26.6-100.1-119.1 156.2 -18.7 -1.7 36.3 19 19 A P + 0 0 70 0, 0.0 2,-0.3 0, 0.0 20,-0.0 -0.361 40.8 165.5 -78.8 149.8 -16.7 -1.1 39.4 20 20 A L - 0 0 28 -2,-0.1 21,-2.2 156,-0.0 20,-1.6 -0.852 22.5-148.9-163.0 129.2 -18.1 -1.3 42.9 21 21 A T E -D 39 0C 83 -2,-0.3 2,-0.5 18,-0.2 18,-0.2 -0.855 11.5-150.9-101.4 133.8 -16.4 -1.5 46.3 22 22 A V E -D 38 0C 0 16,-2.8 16,-1.8 -2,-0.4 2,-0.6 -0.921 4.4-159.0-104.2 133.2 -18.1 -3.4 49.1 23 23 A D E > -D 37 0C 44 -2,-0.5 4,-1.4 14,-0.2 14,-0.2 -0.942 3.8-162.4-113.1 113.5 -17.4 -2.2 52.7 24 24 A F H >>S+ 0 0 3 12,-2.6 5,-2.2 -2,-0.6 4,-0.9 0.841 89.6 54.1 -69.7 -32.9 -18.1 -4.9 55.3 25 25 A E H >45S+ 0 0 114 11,-0.5 3,-0.9 2,-0.2 -1,-0.2 0.940 107.4 50.8 -61.7 -48.7 -18.2 -2.5 58.2 26 26 A A H 345S+ 0 0 84 1,-0.3 -1,-0.2 2,-0.1 -2,-0.2 0.828 112.4 48.5 -56.8 -32.5 -20.8 -0.4 56.5 27 27 A F H 3<5S- 0 0 35 -4,-1.4 -1,-0.3 2,-0.1 -2,-0.2 0.651 112.6-122.6 -80.2 -19.2 -22.7 -3.6 55.9 28 28 A G T <<5 + 0 0 26 -3,-0.9 2,-2.2 -4,-0.9 -3,-0.2 0.612 61.5 146.7 81.3 13.3 -22.4 -4.6 59.6 29 29 A W > < + 0 0 5 -5,-2.2 3,-1.4 1,-0.2 -1,-0.2 -0.499 17.1 165.7 -77.0 76.7 -20.7 -7.9 58.8 30 30 A D T 3 + 0 0 111 -2,-2.2 -1,-0.2 1,-0.3 -5,-0.1 0.419 56.6 75.2 -85.4 3.2 -18.6 -7.5 61.9 31 31 A W T 3 S+ 0 0 6 -3,-0.1 56,-2.9 55,-0.1 2,-0.4 0.552 77.3 99.2 -84.1 -5.7 -17.3 -11.1 62.1 32 32 A I E < +E 86 0D 14 -3,-1.4 54,-0.2 54,-0.2 56,-0.0 -0.682 45.4 177.9 -88.3 130.0 -14.9 -10.2 59.3 33 33 A I E + 0 0 11 52,-2.4 628,-0.3 -2,-0.4 53,-0.2 0.867 58.6 51.6 -93.0 -47.7 -11.4 -9.4 60.2 34 34 A A E S+E 85 0D 28 51,-2.2 51,-3.3 1,-0.2 -1,-0.4 -0.982 96.9 20.7-157.6 147.7 -9.9 -8.8 56.8 35 35 A P - 0 0 35 0, 0.0 -1,-0.2 0, 0.0 -11,-0.1 0.646 56.5-166.8 -83.9 164.0 -10.2 -7.4 54.4 36 36 A K S S+ 0 0 47 1,-0.2 -12,-2.6 -3,-0.1 -11,-0.5 0.813 78.0 15.3 -75.0 -32.1 -12.5 -4.5 55.4 37 37 A R E +D 23 0C 127 -14,-0.2 2,-0.3 -13,-0.1 -14,-0.2 -0.955 67.1 173.1-140.8 154.0 -13.3 -3.6 51.8 38 38 A Y E -D 22 0C 3 -16,-1.8 -16,-2.8 -2,-0.3 2,-1.0 -0.983 40.0 -96.8-157.7 164.7 -12.9 -5.2 48.4 39 39 A K E +D 21 0C 71 -2,-0.3 -18,-0.2 -18,-0.2 64,-0.1 -0.736 42.7 162.4 -89.2 100.3 -13.8 -4.7 44.7 40 40 A A - 0 0 0 -20,-1.6 63,-2.4 -2,-1.0 -19,-0.2 0.817 25.2-167.5 -84.2 -33.2 -17.0 -6.7 44.0 41 41 A N - 0 0 19 -21,-2.2 2,-0.3 61,-0.2 62,-0.1 0.118 5.5-136.7 59.7 173.6 -17.9 -5.0 40.8 42 42 A Y E -B 18 0A 21 -24,-2.4 -24,-3.2 61,-0.1 2,-0.4 -0.981 6.9-110.9-160.1 165.2 -21.4 -5.4 39.2 43 43 A c E +B 17 0A 18 -2,-0.3 137,-0.4 -26,-0.2 2,-0.3 -0.845 36.2 160.0-106.5 136.2 -23.2 -6.0 35.9 44 44 A S E +B 16 0A 21 -28,-2.2 -28,-2.1 -2,-0.4 2,-0.2 -0.981 23.0 68.8-152.1 141.7 -25.4 -3.5 34.1 45 45 A G - 0 0 31 133,-0.4 29,-0.4 -2,-0.3 -31,-0.3 -0.660 69.0 -51.3 139.8 173.9 -26.8 -2.9 30.6 46 46 A E B -c 14 0B 42 -33,-2.9 -31,-1.8 -2,-0.2 2,-0.5 -0.379 30.1-156.8 -81.2 151.6 -29.1 -4.1 27.8 47 47 A d + 0 0 19 25,-0.3 2,-0.3 -33,-0.2 5,-0.1 -0.916 28.1 158.3-125.9 100.6 -29.3 -7.6 26.3 48 48 A E > - 0 0 120 -2,-0.5 3,-2.3 23,-0.1 60,-0.1 -0.816 53.8 -12.8-119.0 165.5 -30.8 -7.5 22.8 49 49 A F T 3 S- 0 0 58 -2,-0.3 -2,-0.0 1,-0.3 0, 0.0 -0.171 120.2 -45.5 49.1-131.7 -30.6 -9.9 19.9 50 50 A V T > S+ 0 0 12 3,-0.0 3,-1.4 4,-0.0 467,-0.3 0.030 90.9 149.1-115.8 26.0 -27.9 -12.5 20.7 51 51 A F T < S- 0 0 12 -3,-2.3 467,-0.2 1,-0.3 57,-0.1 -0.378 73.2 -3.2 -64.3 130.8 -25.4 -9.9 21.8 52 52 A L T 3 S+ 0 0 11 465,-2.6 56,-2.7 1,-0.2 2,-0.6 0.762 93.6 144.8 62.5 27.9 -23.0 -11.1 24.5 53 53 A Q < + 0 0 30 -3,-1.4 464,-0.3 54,-0.2 -1,-0.2 -0.881 27.7 179.3-102.9 120.2 -24.7 -14.5 24.7 54 54 A K + 0 0 79 -2,-0.6 -1,-0.1 54,-0.4 55,-0.1 0.788 66.1 65.2 -84.5 -30.4 -22.4 -17.5 25.4 55 55 A Y >> - 0 0 80 53,-0.4 3,-1.0 1,-0.1 4,-0.6 -0.822 65.5-151.2-104.3 130.8 -25.2 -20.1 25.5 56 56 A P H 3> S+ 0 0 20 0, 0.0 4,-1.6 0, 0.0 3,-0.4 0.811 93.7 72.4 -65.6 -27.8 -27.3 -21.1 22.4 57 57 A H H 3> S+ 0 0 6 153,-1.7 4,-2.2 1,-0.2 5,-0.2 0.845 86.6 65.4 -52.4 -38.5 -30.1 -22.0 24.8 58 58 A T H <> S+ 0 0 16 -3,-1.0 4,-1.7 1,-0.2 -1,-0.2 0.933 106.2 37.9 -55.3 -52.6 -30.8 -18.3 25.6 59 59 A H H X S+ 0 0 22 -4,-0.6 4,-2.7 -3,-0.4 -1,-0.2 0.838 111.0 60.4 -72.3 -31.4 -31.9 -17.3 22.1 60 60 A L H X S+ 0 0 0 -4,-1.6 4,-2.0 2,-0.2 -1,-0.2 0.923 106.6 46.7 -60.0 -43.5 -33.8 -20.6 21.5 61 61 A V H X S+ 0 0 18 -4,-2.2 4,-2.2 2,-0.2 -1,-0.2 0.923 110.6 54.3 -62.6 -43.2 -36.0 -19.9 24.5 62 62 A H H < S+ 0 0 104 -4,-1.7 -2,-0.2 -5,-0.2 -1,-0.2 0.933 107.2 49.5 -55.9 -50.7 -36.5 -16.3 23.3 63 63 A Q H < S+ 0 0 71 -4,-2.7 -1,-0.2 1,-0.2 -2,-0.2 0.923 112.9 47.1 -53.4 -47.0 -37.7 -17.5 19.9 64 64 A A H < S- 0 0 36 -4,-2.0 -1,-0.2 -5,-0.2 -2,-0.2 0.819 88.6-164.0 -67.8 -30.7 -40.2 -19.9 21.5 65 65 A N < - 0 0 120 -4,-2.2 -3,-0.1 -5,-0.2 2,-0.1 0.926 13.0-172.2 43.8 63.6 -41.4 -17.2 23.8 66 66 A P > - 0 0 20 0, 0.0 3,-1.8 0, 0.0 -1,-0.1 -0.432 38.0 -73.0 -79.6 160.5 -43.1 -19.5 26.3 67 67 A R T 3 S+ 0 0 227 1,-0.2 3,-0.1 -2,-0.1 -2,-0.0 -0.236 118.1 16.3 -53.9 135.0 -45.3 -18.1 29.1 68 68 A G T 3 S+ 0 0 45 1,-0.3 2,-0.5 -3,-0.1 87,-0.4 0.338 85.8 135.9 88.7 -7.6 -43.3 -16.4 31.9 69 69 A S < - 0 0 28 -3,-1.8 2,-0.3 85,-0.1 -1,-0.3 -0.631 38.5-158.3 -78.8 124.1 -40.0 -16.1 30.0 70 70 A A - 0 0 87 -2,-0.5 3,-0.1 83,-0.4 83,-0.1 -0.739 31.1 -91.5 -97.0 150.0 -38.3 -12.7 30.5 71 71 A G - 0 0 48 -2,-0.3 -1,-0.2 1,-0.1 38,-0.1 0.011 62.8 -73.3 -48.1 167.7 -35.8 -11.4 28.0 72 72 A P - 0 0 27 0, 0.0 2,-0.3 0, 0.0 -25,-0.3 -0.369 53.2-144.5 -66.1 147.5 -32.2 -12.2 28.7 73 73 A e - 0 0 32 36,-2.0 36,-2.1 -3,-0.1 2,-0.6 -0.848 19.1-107.0-115.8 153.3 -30.7 -10.3 31.6 74 74 A b E + F 0 108D 25 -29,-0.4 34,-0.2 -2,-0.3 -31,-0.1 -0.673 58.5 153.5 -78.8 118.8 -27.2 -8.9 32.1 75 75 A T E - F 0 107D 4 32,-2.8 32,-2.6 -2,-0.6 2,-0.1 -0.922 49.5 -77.0-147.3 161.4 -25.6 -11.2 34.7 76 76 A P E + F 0 106D 29 0, 0.0 30,-0.3 0, 0.0 3,-0.1 -0.432 34.2 177.0 -63.9 134.0 -22.3 -12.5 35.9 77 77 A T E S+ 0 0 50 28,-2.7 2,-0.3 1,-0.3 29,-0.2 0.374 75.3 31.9-113.3 -1.4 -20.9 -15.3 33.7 78 78 A K E - 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