==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=13-SEP-2013 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 06-NOV-12 4HVF . COMPND 2 MOLECULE: GREEN FLUORESCENT PROTEIN BLFP-Y6; . SOURCE 2 ORGANISM_SCIENTIFIC: BRANCHIOSTOMA LANCEOLATUM; . AUTHOR PLETNEV V.Z.,PLETNEVA N.V.,PLETNEV S.V. . 875 8 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 32042.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 631 72.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 44 5.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 392 44.8 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 4 0.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 9 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 16 1.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 54 6.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 72 8.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 38 4.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 4 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 3 3 4 6 4 4 4 2 4 11 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 1 0 0 0 0 0 0 0 0 0 0 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 A S 0 0 122 0, 0.0 2,-1.1 0, 0.0 78,-0.0 0.000 360.0 360.0 360.0 94.8 -32.0 -15.2 -10.8 2 2 A L + 0 0 81 76,-0.2 0, 0.0 72,-0.1 0, 0.0 -0.536 360.0 175.1 -98.4 79.4 -31.7 -13.2 -7.4 3 3 A L - 0 0 36 -2,-1.1 59,-0.1 1,-0.1 2,-0.1 -0.213 36.3 -86.4 -80.3 156.6 -30.8 -9.7 -8.2 4 4 A P + 0 0 2 0, 0.0 105,-0.2 0, 0.0 -1,-0.1 -0.361 39.7 166.9 -71.8 153.0 -30.5 -6.9 -5.7 5 5 A A + 0 0 43 103,-3.0 23,-1.6 1,-0.4 2,-0.3 0.561 69.5 18.5-124.2 -28.2 -33.5 -4.7 -4.6 6 6 A T E -Ab 27 109A 51 102,-1.7 104,-2.1 21,-0.2 -1,-0.4 -0.943 61.8-178.6-137.1 162.3 -32.1 -2.9 -1.6 7 7 A H E -Ab 26 110A 0 19,-2.1 19,-2.7 -2,-0.3 2,-0.3 -0.969 11.4-154.3-153.0 169.4 -28.7 -2.1 -0.1 8 8 A E E -Ab 25 111A 36 102,-2.2 104,-2.8 -2,-0.3 2,-0.4 -0.977 11.5-157.4-144.3 152.9 -27.0 -0.4 2.8 9 9 A L E -Ab 24 112A 4 15,-2.7 15,-1.6 -2,-0.3 2,-0.5 -0.996 16.6-171.9-136.4 133.6 -23.6 1.2 3.1 10 10 A H E -Ab 23 113A 45 102,-2.3 104,-2.6 -2,-0.4 2,-0.4 -0.989 21.5-179.2-114.3 117.6 -21.2 2.1 5.9 11 11 A I E +Ab 22 114A 0 11,-2.2 11,-3.0 -2,-0.5 2,-0.3 -0.965 10.3 156.9-120.1 131.6 -18.4 4.2 4.4 12 12 A F E +Ab 21 115A 55 102,-2.5 104,-2.8 -2,-0.4 2,-0.3 -0.965 13.7 94.7-149.7 165.0 -15.6 5.4 6.6 13 13 A G E -Ab 20 116A 4 7,-1.9 7,-2.5 -2,-0.3 2,-0.3 -0.990 60.8 -77.9 148.3-141.0 -12.0 6.5 6.5 14 14 A S E -Ab 19 117A 0 102,-1.5 104,-2.7 -2,-0.3 2,-0.5 -0.989 13.8-144.2-158.4 153.2 -10.4 9.9 6.3 15 15 A I E > S-Ab 18 118A 0 3,-1.9 3,-2.6 -2,-0.3 104,-0.2 -0.998 96.6 -14.0-122.0 117.2 -9.6 12.8 3.9 16 16 A N T 3 S- 0 0 26 102,-2.2 103,-0.2 -2,-0.5 -1,-0.1 0.897 131.9 -55.3 51.6 42.2 -6.2 14.5 4.7 17 17 A S T 3 S+ 0 0 39 101,-0.5 2,-0.7 1,-0.2 -1,-0.3 0.299 107.8 131.8 72.0 1.4 -6.5 12.6 8.1 18 18 A L E < -A 15 0A 49 -3,-2.6 -3,-1.9 29,-0.1 -1,-0.2 -0.702 56.4-133.6 -82.8 111.8 -9.9 14.1 8.8 19 19 A E E +A 14 0A 89 -2,-0.7 2,-0.3 -5,-0.2 -5,-0.3 -0.408 28.6 177.9 -70.3 145.0 -12.0 11.1 9.9 20 20 A F E -A 13 0A 4 -7,-2.5 -7,-1.9 -2,-0.1 2,-0.3 -0.954 9.5-179.3-139.8 161.6 -15.5 10.6 8.4 21 21 A D E +A 12 0A 27 -2,-0.3 21,-2.9 -9,-0.2 22,-0.6 -0.905 6.2 175.6-162.0 122.8 -18.2 7.9 8.8 22 22 A L E -AC 11 41A 0 -11,-3.0 -11,-2.2 -2,-0.3 2,-0.3 -0.980 5.8-171.0-133.3 154.8 -21.6 7.7 7.2 23 23 A V E +AC 10 40A 62 17,-2.1 17,-2.4 -2,-0.4 2,-0.3 -0.976 20.8 124.0-138.0 151.3 -24.4 5.1 7.2 24 24 A G E -AC 9 39A 16 -15,-1.6 -15,-2.7 -2,-0.3 2,-0.3 -0.982 39.6-108.1 171.3 176.4 -27.5 4.6 5.3 25 25 A R E +AC 8 38A 152 13,-0.7 13,-2.7 -2,-0.3 2,-0.3 -0.767 26.5 172.6-123.4 167.3 -29.8 2.6 3.1 26 26 A G E -AC 7 37A 11 -19,-2.7 -19,-2.1 -2,-0.3 2,-0.3 -0.930 17.5-139.5-158.8-178.0 -30.9 2.6 -0.5 27 27 A T E +AC 6 36A 64 9,-1.3 9,-2.5 -2,-0.3 2,-0.3 -0.898 19.8 173.5-141.4 170.8 -32.8 0.8 -3.3 28 28 A G E - C 0 35A 17 -23,-1.6 7,-0.3 -2,-0.3 -2,-0.0 -0.982 33.9-112.3-168.6-175.5 -32.3 0.2 -7.0 29 29 A N E >>> - C 0 34A 46 5,-2.6 5,-1.9 -2,-0.3 4,-1.2 -0.941 19.0-167.8-132.0 104.2 -33.5 -1.5 -10.1 30 30 A P T 345S+ 0 0 15 0, 0.0 -1,-0.1 0, 0.0 -25,-0.1 0.547 88.5 54.7 -80.9 -5.3 -31.0 -4.0 -11.4 31 31 A K T 345S+ 0 0 165 1,-0.1 37,-0.1 32,-0.1 32,-0.0 0.638 116.4 37.1 -91.6 -16.5 -32.8 -4.4 -14.7 32 32 A E T <45S- 0 0 138 -3,-1.1 179,-0.2 2,-0.1 -1,-0.1 0.450 107.0-118.7-107.1 -16.4 -32.5 -0.6 -15.4 33 33 A G T <5 + 0 0 0 -4,-1.2 178,-2.6 29,-0.6 2,-0.4 0.875 67.0 138.8 73.6 38.7 -29.0 0.1 -13.8 34 34 A Y E < +CD 29 210A 35 -5,-1.9 -5,-2.6 28,-0.2 2,-0.3 -0.925 22.9 164.6-117.5 143.0 -30.5 2.5 -11.3 35 35 A E E -CD 28 209A 0 174,-2.1 174,-2.3 -2,-0.4 2,-0.3 -0.992 12.9-168.4-147.9 152.3 -29.7 3.0 -7.6 36 36 A E E -CD 27 208A 77 -9,-2.5 -9,-1.3 -2,-0.3 2,-0.3 -0.983 8.7-174.1-137.3 151.1 -30.3 5.6 -4.9 37 37 A L E -CD 26 207A 6 170,-2.5 170,-2.9 -2,-0.3 2,-0.5 -0.997 14.2-155.0-149.2 136.4 -28.8 5.9 -1.5 38 38 A H E -CD 25 206A 61 -13,-2.7 -13,-0.7 -2,-0.3 2,-0.3 -0.970 20.3-177.4-115.6 120.8 -29.3 8.1 1.5 39 39 A L E -CD 24 205A 0 166,-2.8 166,-2.5 -2,-0.5 2,-0.4 -0.857 10.4-166.9-116.8 149.7 -26.4 8.5 3.9 40 40 A K E -CD 23 204A 85 -17,-2.4 -17,-2.1 -2,-0.3 164,-0.2 -0.998 27.1-114.1-132.3 138.7 -26.0 10.3 7.2 41 41 A S E -C 22 0A 17 162,-2.8 3,-0.3 -2,-0.4 -19,-0.3 -0.469 13.7-164.0 -68.2 144.8 -22.8 11.2 9.0 42 42 A T S S+ 0 0 78 -21,-2.9 -20,-0.1 1,-0.2 -1,-0.1 0.270 90.7 49.2-104.1 1.8 -22.2 9.5 12.4 43 43 A K S S- 0 0 101 -22,-0.6 -1,-0.2 1,-0.4 -21,-0.0 -0.252 109.5-110.5-133.4 40.2 -19.5 12.1 13.1 44 44 A S S S+ 0 0 93 -3,-0.3 -1,-0.4 159,-0.1 -23,-0.2 -0.062 74.4 10.8 53.0-158.2 -21.0 15.4 12.4 45 45 A A S S- 0 0 44 1,-0.1 2,-0.3 -3,-0.1 158,-0.2 -0.162 72.1-118.8 -60.4 137.1 -20.2 17.7 9.5 46 46 A L - 0 0 11 156,-2.3 -1,-0.1 1,-0.1 -26,-0.1 -0.592 16.0-149.1 -67.8 132.7 -17.9 16.6 6.7 47 47 A Q S S+ 0 0 113 -2,-0.3 2,-0.3 -28,-0.2 -1,-0.1 -0.054 75.8 38.9 -96.1 32.7 -14.9 18.8 6.6 48 48 A F S S- 0 0 1 154,-0.1 80,-0.2 81,-0.1 79,-0.1 -0.942 107.6 -55.2-167.1 166.6 -14.5 18.4 2.9 49 49 A S > - 0 0 2 78,-2.3 3,-2.3 -2,-0.3 4,-0.2 -0.437 38.3-146.3 -58.4 123.6 -16.6 18.3 -0.3 50 50 A P G > S+ 0 0 2 0, 0.0 3,-1.8 0, 0.0 -1,-0.2 0.767 95.6 73.0 -61.1 -20.9 -19.2 15.5 -0.1 51 51 A W G > S+ 0 0 15 1,-0.3 3,-2.0 2,-0.2 -2,-0.1 0.718 79.4 73.3 -66.6 -15.7 -18.7 15.1 -3.8 52 52 A I G < S+ 0 0 0 -3,-2.3 -1,-0.3 1,-0.3 4,-0.2 0.655 88.2 62.8 -74.7 -6.9 -15.3 13.5 -3.1 53 53 A L G <> S+ 0 0 0 -3,-1.8 4,-1.7 -4,-0.2 -1,-0.3 0.450 84.8 119.6 -92.3 -0.6 -17.3 10.5 -1.9 54 54 A V T <4 S+ 0 0 0 -3,-2.0 142,-0.0 1,-0.3 144,-0.0 -0.949 76.4 17.3-100.6 127.8 -18.7 10.0 -5.4 55 55 A P T 4 S+ 0 0 17 0, 0.0 -1,-0.3 0, 0.0 -2,-0.1 -0.895 112.9 76.0-102.1 22.9 -18.3 7.5 -7.0 56 56 A Q T 4 0 0 2 1,-0.2 -2,-0.2 -4,-0.2 -3,-0.1 0.852 360.0 360.0 -52.9 -36.7 -17.2 5.6 -3.8 57 57 A I < 0 0 2 -4,-1.7 -1,-0.2 2,-0.0 -3,-0.1 0.485 360.0 360.0 -89.6 360.0 -20.9 5.5 -2.8 58 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 59 61 A F > 0 0 12 0, 0.0 3,-2.1 0, 0.0 4,-0.1 0.000 360.0 360.0 360.0 72.4 -22.8 -1.0 -6.4 60 62 A Y G > + 0 0 15 1,-0.3 3,-1.7 2,-0.2 14,-0.3 0.528 360.0 87.5 -70.3 -4.8 -22.2 -2.6 -9.7 61 63 A Q G 3 S+ 0 0 0 1,-0.3 -1,-0.3 12,-0.2 10,-0.1 0.764 83.7 59.5 -64.4 -17.4 -24.7 -5.4 -8.6 62 64 A Y G < S+ 0 0 0 -3,-2.1 -29,-0.6 -29,-0.1 -1,-0.3 0.473 85.4 99.0 -84.3 -2.3 -27.4 -3.0 -10.0 63 65 A L < - 0 0 0 -3,-1.7 8,-0.7 -30,-0.1 11,-0.4 -0.799 55.6-165.8 -90.4 110.5 -25.7 -3.1 -13.5 64 66 A P B -M 70 0B 23 0, 0.0 149,-0.6 0, 0.0 6,-0.2 -0.513 19.3-126.7 -79.1 161.5 -27.3 -5.4 -16.1 65 67 A F - 0 0 4 4,-2.6 3,-0.5 1,-0.2 4,-0.3 -0.544 49.8 -61.2 -97.8 172.3 -25.4 -6.2 -19.3 66 68 A P S > S+ 0 0 38 0, 0.0 3,-1.2 0, 0.0 -1,-0.2 -0.148 118.0 18.7 -60.4 149.9 -27.0 -5.8 -22.7 67 69 A D T 3 S- 0 0 174 1,-0.3 -2,-0.0 -3,-0.1 0, 0.0 0.859 133.4 -60.4 65.0 38.0 -30.1 -7.7 -23.7 68 70 A G T 3 S+ 0 0 76 -3,-0.5 -1,-0.3 1,-0.2 2,-0.0 0.521 96.2 145.3 64.6 9.8 -31.1 -8.6 -20.1 69 71 A A < - 0 0 29 -3,-1.2 -4,-2.6 -4,-0.3 2,-0.3 -0.285 53.4-108.7 -69.2 154.6 -27.8 -10.5 -19.4 70 72 A M B -M 64 0B 60 -6,-0.2 -1,-0.1 4,-0.0 -39,-0.0 -0.714 27.0-128.2 -82.5 143.9 -26.2 -10.4 -16.0 71 73 A S > - 0 0 0 -8,-0.7 4,-2.5 -2,-0.3 5,-0.2 -0.386 32.3-104.0 -72.9 166.1 -23.0 -8.5 -15.6 72 74 A P H > S+ 0 0 3 0, 0.0 4,-1.4 0, 0.0 -1,-0.1 0.797 124.1 56.6 -60.6 -25.9 -20.2 -10.4 -13.9 73 75 A F H > S+ 0 0 0 2,-0.2 4,-1.3 1,-0.2 -12,-0.2 0.924 108.1 45.9 -75.9 -42.7 -20.9 -8.4 -10.7 74 76 A Q H > S+ 0 0 3 -11,-0.4 4,-3.0 -14,-0.3 3,-0.4 0.905 109.7 54.6 -64.4 -40.8 -24.5 -9.5 -10.7 75 77 A A H X S+ 0 0 24 -4,-2.5 4,-1.7 1,-0.2 6,-0.2 0.866 105.6 53.0 -59.6 -36.2 -23.5 -13.1 -11.3 76 78 A A H < S+ 0 0 3 -4,-1.4 6,-2.2 2,-0.2 -1,-0.2 0.782 112.5 43.7 -74.5 -27.0 -21.2 -13.0 -8.4 77 79 A M H >< S+ 0 0 10 -4,-1.3 3,-1.6 -3,-0.4 -2,-0.2 0.898 108.3 61.0 -73.4 -45.6 -24.0 -11.8 -6.2 78 80 A N H 3< S+ 0 0 61 -4,-3.0 -2,-0.2 1,-0.3 -76,-0.2 0.861 113.8 33.0 -52.9 -41.7 -26.4 -14.3 -7.7 79 81 A D T 3< S- 0 0 77 -4,-1.7 -1,-0.3 -5,-0.1 -2,-0.2 0.324 107.1-120.1-101.9 6.8 -24.4 -17.3 -6.6 80 82 A G S < S+ 0 0 55 -3,-1.6 25,-0.1 -4,-0.1 -3,-0.1 0.232 82.6 112.7 83.9 -11.4 -23.1 -15.8 -3.4 81 83 A S - 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