==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=20-JUL-2011 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER IMMUNOGLOBULIN 25-OCT-96 1IGT . COMPND 2 MOLECULE: IGG2A INTACT ANTIBODY - MAB231; . SOURCE 2 ORGANISM_SCIENTIFIC: MUS MUSCULUS; . AUTHOR L.J.HARRIS,S.B.LARSON,K.W.HASEL,A.MCPHERSON . 1316 4 17 12 5 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 64882.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 857 65.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 21 1.6 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 483 36.7 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 10 0.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 10 0.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 11 0.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 2 0.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 1 0.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 185 14.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 100 7.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 45 3.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 9 0.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 8 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 2 1 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 17 16 11 6 13 20 4 0 5 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 1 0 13 7 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 A D 0 0 124 0, 0.0 2,-0.7 0, 0.0 26,-0.1 0.000 360.0 360.0 360.0 160.1 1.6 -84.3 45.6 2 2 A I - 0 0 10 93,-0.4 2,-0.4 91,-0.1 88,-0.2 -0.969 360.0-154.1-110.7 108.7 -0.9 -83.4 48.3 3 3 A V - 0 0 92 -2,-0.7 23,-1.4 96,-0.0 2,-0.3 -0.738 5.2-146.2 -88.4 133.5 -2.5 -80.2 47.2 4 4 A L B -A 25 0A 13 94,-0.6 2,-0.4 -2,-0.4 21,-0.2 -0.716 4.0-151.3 -93.7 152.2 -3.9 -78.1 50.0 5 5 A T - 0 0 78 19,-2.6 2,-0.3 -2,-0.3 19,-0.2 -0.798 9.8-169.6-131.4 92.8 -7.1 -76.0 49.5 6 6 A Q - 0 0 15 93,-0.6 17,-0.2 -2,-0.4 95,-0.2 -0.598 23.8-126.0 -80.6 139.2 -7.4 -72.8 51.5 7 7 A S B +B 22 0B 45 15,-2.4 15,-2.4 -2,-0.3 2,-0.2 -0.993 68.9 19.2-143.7 144.3 -10.8 -71.2 51.5 8 8 A P - 0 0 47 0, 0.0 3,-0.1 0, 0.0 14,-0.1 0.540 66.0-140.9 -78.0-148.8 -12.0 -68.6 50.9 9 9 A S S S+ 0 0 94 1,-0.4 94,-2.2 -2,-0.2 2,-0.3 0.645 80.3 3.5-109.4 -21.1 -9.6 -66.6 48.9 10 10 A S E -e 103 0C 71 92,-0.3 -1,-0.4 75,-0.0 2,-0.3 -0.978 65.4-169.0-159.7 151.8 -10.2 -63.2 50.5 11 11 A L E -e 104 0C 54 92,-2.4 94,-3.1 -2,-0.3 2,-0.4 -0.998 8.8-161.4-149.5 150.3 -12.3 -61.9 53.4 12 12 A S E +e 105 0C 46 -2,-0.3 2,-0.3 92,-0.2 94,-0.2 -0.981 23.1 151.8-130.6 140.0 -13.4 -58.6 54.9 13 13 A A E -e 106 0C 18 92,-1.3 94,-1.6 -2,-0.4 3,-0.1 -0.974 42.0 -92.8-161.8 160.7 -14.8 -58.1 58.4 14 14 A S > - 0 0 60 -2,-0.3 3,-1.5 92,-0.2 64,-0.3 -0.339 53.4 -82.9 -77.4 162.8 -15.0 -55.5 61.1 15 15 A L T 3 S+ 0 0 87 92,-0.3 -1,-0.2 1,-0.2 64,-0.1 -0.199 115.7 24.7 -59.2 153.1 -12.5 -55.0 63.9 16 16 A G T 3 S+ 0 0 33 62,-1.5 61,-1.1 1,-0.2 -1,-0.2 0.215 91.9 133.4 75.9 -19.5 -13.0 -57.3 66.9 17 17 A D < - 0 0 71 -3,-1.5 61,-1.8 60,-0.2 2,-0.5 -0.101 56.5-129.2 -61.0 161.0 -14.8 -59.8 64.7 18 18 A T - 0 0 92 58,-0.2 2,-0.3 59,-0.2 58,-0.2 -0.973 32.5-175.1-112.1 128.7 -13.9 -63.5 65.0 19 19 A I E - C 0 75B 15 56,-2.3 56,-2.0 -2,-0.5 2,-0.5 -0.976 16.4-144.8-132.1 146.8 -13.2 -65.0 61.6 20 20 A T E - C 0 74B 86 -2,-0.3 2,-0.3 54,-0.2 54,-0.2 -0.923 10.9-174.7-119.6 119.6 -12.4 -68.6 60.4 21 21 A I E - C 0 73B 0 52,-1.9 52,-2.2 -2,-0.5 2,-0.3 -0.725 17.5-159.4-100.5 149.4 -10.1 -69.5 57.5 22 22 A T E -BC 7 72B 34 -15,-2.4 -15,-2.4 -2,-0.3 2,-0.3 -0.942 14.8-164.0-135.7 159.9 -9.9 -73.1 56.5 23 23 A a E - C 0 71B 0 48,-2.9 48,-2.5 -2,-0.3 2,-0.3 -0.967 6.7-167.6-136.6 149.9 -7.6 -75.6 54.6 24 24 A H E - C 0 70B 91 -2,-0.3 -19,-2.6 46,-0.2 2,-0.4 -0.974 8.1-147.9-136.6 154.0 -8.3 -79.0 53.2 25 25 A A B -A 4 0A 13 44,-1.4 -21,-0.2 -2,-0.3 4,-0.1 -0.934 16.6-141.2-129.8 146.9 -6.0 -81.7 51.9 26 26 A S S S+ 0 0 68 -23,-1.4 -1,-0.2 -2,-0.4 2,-0.1 0.993 93.7 28.8 -59.3 -73.9 -6.2 -84.3 49.3 27 27 A Q S S- 0 0 124 1,-0.1 2,-0.1 -26,-0.1 -25,-0.0 -0.402 104.5 -85.4 -86.6 161.4 -4.5 -87.0 51.3 28 28 A N - 0 0 88 1,-0.1 41,-0.2 -2,-0.1 -1,-0.1 -0.428 38.4-179.8 -66.9 136.4 -4.5 -87.3 55.1 29 29 A I > - 0 0 0 39,-2.8 3,-2.7 1,-0.2 2,-1.2 0.055 25.7-145.7-125.3 17.8 -1.7 -85.2 56.8 30 30 A N T 3 - 0 0 58 38,-0.3 38,-2.8 1,-0.3 -1,-0.2 -0.277 69.6 -35.0 56.0 -87.0 -2.5 -86.2 60.3 31 31 A V T 3 S+ 0 0 47 -2,-1.2 2,-2.0 36,-0.2 20,-0.7 0.250 98.6 124.3-151.0 10.9 -1.7 -83.0 62.2 32 32 A W < + 0 0 74 -3,-2.7 59,-2.0 18,-0.2 2,-0.4 -0.492 37.5 128.5 -79.9 74.7 1.2 -81.7 60.2 33 33 A L E -F 90 0C 0 -2,-2.0 18,-1.1 57,-0.2 57,-0.2 -0.988 35.3-168.2-136.7 131.0 -0.1 -78.2 59.3 34 34 A S E -F 89 0C 1 55,-2.4 55,-1.1 -2,-0.4 2,-0.4 -0.562 9.3-148.2-109.2 169.7 1.4 -74.8 59.7 35 35 A W E -FG 88 48C 0 13,-1.7 12,-2.8 53,-0.2 13,-1.6 -0.946 14.1-168.5-144.1 122.0 -0.1 -71.4 59.4 36 36 A Y E -FG 87 46C 0 51,-2.6 51,-1.3 -2,-0.4 2,-0.5 -0.801 10.7-149.0-113.4 153.0 1.9 -68.3 58.2 37 37 A Q E -FG 86 45C 25 8,-2.2 8,-1.9 -2,-0.3 2,-0.5 -0.980 10.9-176.6-125.1 123.8 1.1 -64.6 58.2 38 38 A Q E -F 85 0C 16 47,-2.6 47,-1.4 -2,-0.5 3,-0.1 -0.894 10.4-160.0-122.6 95.8 2.4 -62.3 55.5 39 39 A K - 0 0 78 -2,-0.5 3,-0.5 4,-0.5 45,-0.2 -0.666 50.6 -76.5 -76.3 129.9 1.5 -58.6 56.0 40 40 A P S S+ 0 0 72 0, 0.0 2,-0.7 0, 0.0 -1,-0.1 0.070 116.1 0.5 -29.4 107.0 1.8 -56.7 52.7 41 41 A G S S+ 0 0 78 1,-0.3 -2,-0.0 -3,-0.1 -3,-0.0 -0.619 110.6 91.1 109.2 -69.6 5.5 -56.1 52.1 42 42 A N S S- 0 0 87 -2,-0.7 -1,-0.3 -3,-0.5 3,-0.1 0.076 83.2 -93.6 -56.3 167.1 7.1 -57.8 55.2 43 43 A I - 0 0 15 1,-0.1 -4,-0.5 -3,-0.1 2,-0.2 -0.290 46.5 -88.7 -80.1 164.9 8.2 -61.5 55.2 44 44 A P - 0 0 0 0, 0.0 2,-0.4 0, 0.0 -6,-0.2 -0.568 39.7-158.5 -78.4 140.2 6.0 -64.4 56.4 45 45 A K E -G 37 0C 99 -8,-1.9 -8,-2.2 -2,-0.2 2,-0.4 -0.906 25.9-105.6-115.9 146.9 6.2 -65.3 60.0 46 46 A L E +G 36 0C 8 275,-0.5 12,-0.2 -2,-0.4 -10,-0.2 -0.608 39.3 171.0 -73.4 128.7 5.3 -68.6 61.5 47 47 A L E + 0 0 10 -12,-2.8 8,-2.4 -2,-0.4 2,-0.3 0.813 61.6 4.3-105.0 -45.6 2.0 -68.5 63.4 48 48 A I E -GH 35 54C 0 -13,-1.6 -13,-1.7 6,-0.2 -1,-0.3 -0.998 55.5-176.6-149.1 140.8 1.1 -72.1 64.2 49 49 A Y E > + H 0 53C 56 4,-2.8 4,-0.7 -2,-0.3 -15,-0.1 -0.752 69.5 31.7-126.7 170.7 2.6 -75.5 63.8 50 50 A K T >4 S- 0 0 64 267,-0.4 2,-4.0 -2,-0.3 3,-2.1 0.904 120.7 -79.4 41.4 60.6 1.3 -79.0 64.6 51 51 A A T 34 S+ 0 0 2 -18,-1.1 -1,-0.2 -20,-0.7 14,-0.2 -0.025 126.8 22.1 48.5 -52.7 -2.3 -77.8 63.8 52 52 A S T 34 S+ 0 0 63 -2,-4.0 12,-0.5 -21,-0.1 -1,-0.3 0.658 88.1 116.6-113.3 -22.2 -2.9 -76.1 67.1 53 53 A N E << -H 49 0C 52 -3,-2.1 -4,-2.8 -4,-0.7 2,-0.2 -0.270 57.2-144.2 -56.3 110.4 0.5 -75.3 68.4 54 54 A L E -H 48 0C 64 -2,-0.3 -6,-0.2 -6,-0.2 2,-0.1 -0.542 13.2-130.6 -75.2 142.3 0.9 -71.6 68.7 55 55 A H - 0 0 35 -8,-2.4 3,-0.3 -2,-0.2 -1,-0.1 -0.421 41.4 -70.5 -87.3 166.3 4.3 -70.2 67.9 56 56 A T S S+ 0 0 118 1,-0.2 2,-2.0 -2,-0.1 -1,-0.2 -0.105 118.4 17.2 -52.0 151.8 6.1 -67.7 70.1 57 57 A G S S+ 0 0 70 1,-0.2 -1,-0.2 -3,-0.1 3,-0.1 -0.317 82.8 132.0 82.8 -60.0 4.6 -64.2 70.1 58 58 A V - 0 0 19 -2,-2.0 -1,-0.2 -3,-0.3 4,-0.1 0.040 60.4-127.6 -31.7 99.4 1.3 -65.2 68.6 59 59 A P > - 0 0 58 0, 0.0 3,-1.8 0, 0.0 -1,-0.1 -0.285 27.6-103.0 -55.8 142.8 -1.2 -63.4 71.0 60 60 A S T 3 S+ 0 0 111 1,-0.3 -2,-0.1 -3,-0.1 -6,-0.0 0.679 110.4 83.9 -37.5 -32.9 -3.8 -65.9 72.3 61 61 A R T 3 S+ 0 0 52 16,-0.1 15,-2.3 14,-0.1 2,-0.4 0.518 75.8 81.2 -55.6 -8.4 -6.5 -64.5 69.9 62 62 A F E < -D 75 0B 5 -3,-1.8 2,-0.4 13,-0.2 13,-0.2 -0.868 61.9-178.0-100.8 135.0 -5.1 -66.8 67.1 63 63 A S E -D 74 0B 44 11,-2.2 11,-1.5 -2,-0.4 2,-0.2 -0.943 10.5-166.2-142.3 117.4 -6.3 -70.4 67.3 64 64 A G E +D 73 0B 10 -12,-0.5 2,-0.3 -2,-0.4 9,-0.2 -0.653 21.6 152.3 -95.8 158.3 -5.4 -73.3 65.1 65 65 A S E +D 72 0B 65 7,-1.7 7,-2.2 -2,-0.2 2,-0.2 -0.976 12.7 83.8-169.5 175.6 -7.4 -76.5 65.0 66 66 A G E -D 71 0B 29 -2,-0.3 2,-0.2 5,-0.3 5,-0.2 -0.515 41.2-138.1 110.7 179.1 -8.4 -79.6 63.0 67 67 A S E > +D 70 0B 93 3,-1.5 2,-1.4 -2,-0.2 3,-0.8 -0.856 62.8 11.7-177.9 140.6 -7.0 -83.0 62.1 68 68 A G T 3 S- 0 0 18 -38,-2.8 -39,-2.8 -2,-0.2 -38,-0.3 -0.599 128.3 -15.0 93.2 -82.3 -6.7 -85.3 59.1 69 69 A T T 3 S+ 0 0 62 -2,-1.4 -44,-1.4 1,-0.3 2,-0.5 0.539 123.5 60.4-132.4 -22.6 -7.7 -83.3 56.1 70 70 A G E < +CD 24 67B 22 -3,-0.8 -3,-1.5 -46,-0.2 2,-0.3 -0.934 56.1 169.2-115.5 131.6 -9.5 -80.2 57.2 71 71 A F E -CD 23 66B 0 -48,-2.5 -48,-2.9 -2,-0.5 2,-0.3 -0.952 12.6-159.4-133.9 156.6 -7.8 -77.6 59.4 72 72 A T E -CD 22 65B 49 -7,-2.2 -7,-1.7 -2,-0.3 2,-0.4 -0.985 9.3-153.2-141.7 146.8 -8.9 -74.2 60.4 73 73 A L E -CD 21 64B 0 -52,-2.2 -52,-1.9 -2,-0.3 2,-0.4 -0.899 18.8-165.3-111.6 144.6 -7.3 -71.0 61.7 74 74 A T E -CD 20 63B 28 -11,-1.5 -11,-2.2 -2,-0.4 2,-0.5 -0.971 8.8-164.2-142.1 125.2 -9.3 -68.7 63.8 75 75 A I E -CD 19 62B 0 -56,-2.0 -56,-2.3 -2,-0.4 2,-0.3 -0.906 8.1-160.1-108.9 133.1 -8.7 -65.1 64.8 76 76 A S S S+ 0 0 41 -15,-2.3 3,-0.2 -2,-0.5 -58,-0.2 -0.783 70.5 7.7-107.3 153.7 -10.7 -63.4 67.6 77 77 A S S S- 0 0 63 -61,-1.1 -1,-0.2 -2,-0.3 -60,-0.2 0.889 90.0-124.8 39.1 63.5 -11.0 -59.6 68.1 78 78 A L - 0 0 7 -61,-1.8 -62,-1.5 -64,-0.3 -1,-0.2 -0.049 26.3-159.1 -38.7 112.1 -9.3 -58.7 64.8 79 79 A Q > - 0 0 79 -3,-0.2 3,-0.5 -64,-0.1 4,-0.2 -0.925 29.4-111.1-102.0 126.8 -6.5 -56.4 65.8 80 80 A P G > S+ 0 0 74 0, 0.0 3,-1.8 0, 0.0 2,-1.1 0.654 113.0 67.4 -20.6 -49.0 -5.2 -54.1 62.9 81 81 A E G 3 S+ 0 0 76 1,-0.3 -42,-0.1 -42,-0.0 -3,-0.0 0.020 85.3 73.4 -73.2 31.5 -1.9 -56.0 62.7 82 82 A D G < + 0 0 1 -2,-1.1 -1,-0.3 -3,-0.5 2,-0.2 0.620 62.1 112.5-111.0 -28.9 -3.7 -59.1 61.4 83 83 A I < + 0 0 33 -3,-1.8 2,-0.3 -4,-0.2 22,-0.1 -0.257 52.1 98.4 -52.4 106.1 -4.3 -57.7 58.0 84 84 A A S S- 0 0 0 -2,-0.2 20,-2.6 -45,-0.2 2,-0.4 -0.849 78.7 -80.0-165.6-164.0 -2.1 -59.9 55.9 85 85 A T E -FI 38 103C 21 -47,-1.4 -47,-2.6 -2,-0.3 2,-0.4 -0.962 40.0-150.7-120.4 135.7 -2.4 -63.0 53.8 86 86 A Y E -FI 37 102C 0 16,-2.9 16,-1.6 -2,-0.4 2,-0.4 -0.866 12.8-176.2-110.1 144.1 -2.5 -66.4 55.5 87 87 A Y E -F 36 0C 5 -51,-1.3 -51,-2.6 -2,-0.4 2,-0.3 -0.990 11.5-152.6-136.8 142.2 -1.3 -69.6 54.0 88 88 A a E -F 35 0C 0 -2,-0.4 11,-2.2 -53,-0.2 2,-0.3 -0.742 13.4-166.6-107.9 160.9 -1.5 -73.1 55.4 89 89 A Q E -FJ 34 98C 0 -55,-1.1 -55,-2.4 -2,-0.3 9,-0.2 -0.933 15.5-134.8-154.8 127.1 1.1 -75.8 54.6 90 90 A Q E -F 33 0C 1 7,-1.2 7,-0.4 -2,-0.3 -57,-0.2 -0.471 14.9-176.2 -75.3 152.4 1.1 -79.5 55.1 91 91 A G + 0 0 4 -59,-2.0 -58,-0.1 -62,-0.2 -1,-0.1 0.004 54.4 103.3-137.4 25.2 4.3 -81.1 56.4 92 92 A Q S S- 0 0 75 -60,-0.4 2,-0.3 1,-0.2 4,-0.1 0.909 90.5 -6.4 -75.4 -46.6 3.3 -84.7 56.2 93 93 A S S S- 0 0 56 2,-0.2 -1,-0.2 -91,-0.1 -91,-0.1 -0.905 88.9 -45.5-145.3 173.3 5.2 -85.6 53.1 94 94 A Y S S+ 0 0 36 -2,-0.3 2,-0.1 2,-0.1 -3,-0.0 -0.690 101.4 44.3 -92.5 143.3 7.4 -84.3 50.3 95 95 A P S S- 0 0 8 0, 0.0 2,-0.5 0, 0.0 -93,-0.4 0.533 84.5-132.3 -79.5 160.4 7.1 -82.1 48.5 96 96 A L - 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