==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=8-FEB-2013 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER UNKNOWN FUNCTION 14-JAN-13 4IQZ . COMPND 2 MOLECULE: DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA'; . SOURCE 2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI; . AUTHOR V.BHANDARI,S.N.SUGIMAN-MARANGOS,H.S.NAUSHAD,R.S.GUPTA,M.S.JU . 851 18 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 38036.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 532 62.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 10 1.2 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 316 37.1 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 15 1.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 5 0.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 2 0.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 78 9.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 62 7.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 13 1.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 5 0.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 14 25 17 2 18 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 1 0 4 10 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 34 A A 0 0 111 0, 0.0 3,-0.5 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 -50.8 78.7 38.0 38.6 2 35 A A > + 0 0 87 1,-0.2 3,-1.1 2,-0.2 5,-0.1 0.850 360.0 57.5 -72.6 -36.2 81.4 35.4 39.3 3 36 A E T 3 S+ 0 0 187 1,-0.2 -1,-0.2 3,-0.1 3,-0.0 0.699 110.8 45.8 -64.8 -20.9 78.9 33.1 41.1 4 37 A S T 3 S+ 0 0 5 -3,-0.5 72,-2.6 72,-0.0 2,-0.3 -0.075 110.7 47.1-121.0 34.5 76.7 33.1 38.0 5 38 A S E < -A 75 0A 33 -3,-1.1 2,-0.5 70,-0.2 70,-0.2 -0.968 67.6-130.5-156.0 167.6 79.2 32.5 35.1 6 39 A I E +A 74 0A 5 68,-2.2 67,-3.2 -2,-0.3 68,-1.3 -0.995 19.6 179.0-126.3 125.1 82.1 30.2 34.2 7 40 A Q E -A 72 0A 101 -2,-0.5 65,-0.3 65,-0.3 2,-0.1 -0.994 27.3-132.7-120.9 117.9 85.3 31.6 33.0 8 41 A V - 0 0 1 63,-2.3 62,-2.5 -2,-0.5 63,-0.1 -0.400 5.1-150.4 -74.8 153.1 87.9 28.9 32.3 9 42 A K S S+ 0 0 155 60,-0.2 2,-0.3 61,-0.1 60,-0.2 0.615 76.7 23.8 -98.4 -14.6 91.5 29.4 33.6 10 43 A N S S- 0 0 95 58,-0.1 60,-0.1 59,-0.1 61,-0.1 -0.931 90.7 -88.3-141.1 167.2 93.3 27.5 30.9 11 44 A K + 0 0 95 -2,-0.3 2,-0.3 58,-0.1 57,-0.2 -0.345 65.3 115.8 -71.5 159.2 92.8 26.4 27.3 12 45 A G E -E 67 0B 13 55,-2.2 55,-3.1 30,-0.1 2,-0.4 -0.968 61.1 -72.9 170.5-165.5 91.0 23.2 26.7 13 46 A S E -EF 66 41B 26 28,-2.8 28,-2.8 -2,-0.3 2,-0.4 -0.921 41.9-113.6-118.5 150.4 87.8 21.8 25.1 14 47 A I E - F 0 40B 2 51,-2.5 50,-3.6 -2,-0.4 2,-0.4 -0.655 30.2-174.2 -80.2 130.3 84.3 22.0 26.5 15 48 A K E - F 0 39B 56 24,-2.7 24,-2.9 -2,-0.4 2,-0.4 -0.983 3.8-167.3-124.8 121.1 82.8 18.7 27.6 16 49 A L E - F 0 38B 16 -2,-0.4 2,-0.4 22,-0.2 3,-0.2 -0.872 2.3-161.0-106.2 135.0 79.2 18.7 28.7 17 50 A S E S+ F 0 37B 39 20,-3.1 20,-1.7 -2,-0.4 269,-0.0 -0.918 70.2 3.9-117.3 148.9 77.6 15.8 30.4 18 51 A N S S+ 0 0 78 -2,-0.4 17,-0.4 18,-0.2 2,-0.4 0.901 94.5 156.2 50.7 51.3 73.9 15.0 30.8 19 52 A V - 0 0 46 -3,-0.2 2,-0.4 17,-0.1 -1,-0.2 -0.840 35.5-176.7-114.7 139.0 73.0 18.1 28.7 20 53 A K + 0 0 73 -2,-0.4 12,-3.5 2,-0.0 13,-0.8 -0.995 22.4 172.9-122.3 130.1 70.1 19.1 26.5 21 54 A S E +B 31 0A 57 -2,-0.4 2,-0.3 10,-0.3 10,-0.2 -0.953 12.0 178.7-134.5 156.9 70.6 22.4 24.7 22 55 A V E -B 30 0A 37 8,-1.9 8,-3.3 -2,-0.3 2,-0.5 -0.979 32.0-113.5-151.2 153.4 68.7 24.4 22.1 23 56 A V E -B 29 0A 22 -2,-0.3 6,-0.2 6,-0.2 148,-0.1 -0.822 36.7-153.2 -91.1 129.0 69.3 27.7 20.3 24 57 A N > - 0 0 12 4,-3.0 3,-1.5 -2,-0.5 146,-0.1 -0.245 33.2 -89.3 -96.0-171.8 66.6 30.3 21.2 25 58 A S T 3 S+ 0 0 7 1,-0.3 578,-0.2 143,-0.2 662,-0.1 0.584 123.0 59.7 -74.7 -10.1 65.2 33.3 19.4 26 59 A S T 3 S- 0 0 32 2,-0.1 -1,-0.3 576,-0.1 3,-0.1 0.325 120.1-105.5 -96.5 3.4 67.9 35.6 20.9 27 60 A G S < S+ 0 0 0 -3,-1.5 636,-2.7 1,-0.3 2,-0.4 0.670 76.1 140.5 81.4 16.7 70.7 33.5 19.4 28 61 A K - 0 0 39 634,-0.2 -4,-3.0 30,-0.0 2,-0.8 -0.749 54.1-135.4 -96.9 140.1 71.6 32.0 22.7 29 62 A L E -BC 23 59A 6 30,-0.7 30,-2.9 -2,-0.4 2,-0.4 -0.846 35.6-165.4 -90.8 109.0 72.5 28.4 23.2 30 63 A V E -BC 22 58A 5 -8,-3.3 -8,-1.9 -2,-0.8 2,-0.4 -0.836 19.3-132.2-111.5 136.4 70.5 27.5 26.2 31 64 A I E +B 21 0A 0 26,-2.8 25,-2.7 -2,-0.4 -10,-0.3 -0.681 22.0 177.4 -83.3 128.7 70.8 24.5 28.5 32 65 A T + 0 0 27 -12,-3.5 2,-0.2 -2,-0.4 -11,-0.2 0.266 53.0 91.3-117.7 10.7 67.4 22.9 29.2 33 66 A S - 0 0 16 -13,-0.8 3,-0.2 21,-0.1 23,-0.2 -0.678 64.4-146.7-102.1 160.5 68.6 20.0 31.3 34 67 A R S S+ 0 0 91 -2,-0.2 21,-0.3 1,-0.1 -15,-0.1 0.561 88.2 44.7-110.4 -9.8 68.9 20.2 35.1 35 68 A N S S+ 0 0 116 -17,-0.4 -1,-0.1 19,-0.1 2,-0.1 -0.043 76.5 151.5-123.5 35.1 71.9 18.1 36.1 36 69 A T - 0 0 1 17,-0.5 17,-2.4 -3,-0.2 2,-0.4 -0.388 27.6-160.8 -71.2 136.4 74.4 19.2 33.5 37 70 A E E -FG 17 52B 41 -20,-1.7 -20,-3.1 15,-0.2 2,-0.5 -0.968 2.3-157.9-113.9 137.8 78.1 19.2 34.3 38 71 A L E -FG 16 51B 0 13,-3.3 13,-2.9 -2,-0.4 2,-0.4 -0.963 13.9-176.9-113.0 129.7 80.6 21.2 32.2 39 72 A K E -FG 15 50B 57 -24,-2.9 -24,-2.7 -2,-0.5 2,-0.6 -0.959 24.5-140.5-125.1 145.4 84.2 20.2 32.2 40 73 A L E +FG 14 49B 0 9,-2.9 8,-3.7 -2,-0.4 9,-1.4 -0.935 37.2 173.6 -97.6 118.9 87.3 21.7 30.6 41 74 A I E -FG 13 47B 22 -28,-2.8 -28,-2.8 -2,-0.6 6,-0.2 -0.783 29.5-119.4-120.5 166.3 89.3 18.8 29.2 42 75 A D > - 0 0 20 4,-1.9 3,-1.4 -2,-0.3 -30,-0.1 -0.305 49.6 -85.2 -88.5-173.7 92.5 18.4 27.2 43 76 A E T 3 S+ 0 0 173 1,-0.3 -1,-0.0 2,-0.1 -31,-0.0 0.533 129.2 50.8 -71.1 -3.8 92.5 16.6 23.8 44 77 A F T 3 S- 0 0 165 2,-0.1 -1,-0.3 0, 0.0 0, 0.0 0.266 121.7-100.2-119.1 8.2 92.9 13.2 25.6 45 78 A G < + 0 0 44 -3,-1.4 2,-0.2 1,-0.3 -2,-0.1 0.450 69.6 148.2 90.7 3.2 90.1 13.6 28.0 46 79 A R - 0 0 177 1,-0.1 -4,-1.9 2,-0.0 2,-0.4 -0.521 49.0-120.7 -71.1 134.2 92.0 14.7 31.1 47 80 A T E +G 41 0B 71 -6,-0.2 -6,-0.2 -2,-0.2 3,-0.1 -0.623 36.3 169.5 -80.8 131.0 90.0 17.0 33.3 48 81 A K E S+ 0 0 95 -8,-3.7 2,-0.3 1,-0.4 -7,-0.2 0.784 72.0 1.3 -98.8 -44.6 91.3 20.5 34.0 49 82 A E E -G 40 0B 23 -9,-1.4 -9,-2.9 2,-0.0 -1,-0.4 -0.985 61.8-162.0-146.1 150.7 88.2 22.1 35.5 50 83 A S E +G 39 0B 56 -2,-0.3 2,-0.3 -11,-0.2 -11,-0.2 -0.997 12.3 169.9-138.2 140.1 84.7 20.9 36.4 51 84 A Y E -G 38 0B 63 -13,-2.9 -13,-3.3 -2,-0.4 2,-0.3 -0.997 32.6-125.6-145.9 147.3 81.5 22.8 37.1 52 85 A K E -G 37 0B 142 -2,-0.3 -15,-0.2 -15,-0.2 -17,-0.0 -0.717 29.7-156.5 -81.3 143.7 77.8 22.1 37.6 53 86 A V - 0 0 3 -17,-2.4 -17,-0.5 -2,-0.3 3,-0.1 -0.992 12.7-128.0-127.7 124.6 75.8 24.3 35.2 54 87 A P > - 0 0 49 0, 0.0 3,-2.0 0, 0.0 -23,-0.2 -0.257 31.0 -85.7 -78.6 158.1 72.2 25.1 36.0 55 88 A Y T 3 S+ 0 0 20 -21,-0.3 -23,-0.2 1,-0.2 -21,-0.1 -0.299 115.7 27.3 -46.1 132.8 69.1 24.8 33.9 56 89 A G T 3 S+ 0 0 15 -25,-2.7 -1,-0.2 1,-0.3 -24,-0.1 0.301 84.7 144.4 91.7 -9.5 68.6 27.9 31.7 57 90 A A < - 0 0 0 -3,-2.0 -26,-2.8 -27,-0.1 2,-0.4 -0.284 46.7-129.9 -60.4 148.9 72.3 28.8 31.6 58 91 A V E -CD 30 75A 32 17,-3.1 17,-2.2 -28,-0.2 2,-0.4 -0.858 17.8-150.9-103.3 132.4 73.6 30.2 28.3 59 92 A L E -CD 29 74A 2 -30,-2.9 -30,-0.7 -2,-0.4 15,-0.2 -0.870 16.0-167.9-103.1 139.0 76.7 28.7 26.7 60 93 A A S S+ 0 0 5 13,-2.0 2,-0.4 -2,-0.4 14,-0.1 0.638 85.8 35.0 -90.4 -19.0 79.1 30.6 24.5 61 94 A K S S- 0 0 39 12,-0.9 -1,-0.2 -32,-0.1 3,-0.1 -1.000 80.2-142.5-136.4 134.0 80.7 27.4 23.5 62 95 A G > - 0 0 1 -2,-0.4 3,-2.0 1,-0.2 -48,-0.3 -0.254 45.7 -58.1 -87.3 179.6 79.1 24.0 22.9 63 96 A D T 3 S+ 0 0 64 1,-0.3 -48,-0.2 -50,-0.1 -1,-0.2 -0.316 124.6 18.9 -59.4 132.0 80.3 20.5 23.7 64 97 A G T 3 S+ 0 0 37 -50,-3.6 -1,-0.3 1,-0.3 2,-0.2 0.320 89.8 135.8 89.5 -8.8 83.5 19.7 21.9 65 98 A E < - 0 0 35 -3,-2.0 -51,-2.5 -51,-0.1 -1,-0.3 -0.528 54.0-126.3 -77.5 138.2 84.5 23.4 21.3 66 99 A Q E -E 13 0B 78 -53,-0.2 2,-0.3 -2,-0.2 -53,-0.3 -0.610 31.3-174.8 -78.7 139.7 88.1 24.5 21.9 67 100 A V E -E 12 0B 11 -55,-3.1 -55,-2.2 -2,-0.3 2,-0.2 -0.951 17.0-130.8-134.7 155.8 88.4 27.5 24.3 68 101 A A > - 0 0 61 -2,-0.3 3,-1.5 -57,-0.2 -60,-0.2 -0.635 41.7 -79.3-102.2 162.7 91.4 29.6 25.4 69 102 A G T 3 S+ 0 0 43 1,-0.2 -60,-0.2 -2,-0.2 -1,-0.1 -0.310 115.5 26.4 -58.3 141.1 92.3 30.6 29.0 70 103 A G T 3 S+ 0 0 39 -62,-2.5 2,-0.3 1,-0.3 -1,-0.2 0.380 86.9 135.8 89.6 -4.9 90.3 33.5 30.3 71 104 A E < - 0 0 34 -3,-1.5 -63,-2.3 -63,-0.1 2,-0.4 -0.610 53.9-132.4 -83.8 133.7 87.3 32.9 28.1 72 105 A T E +A 7 0A 9 585,-0.5 -65,-0.3 -2,-0.3 3,-0.1 -0.721 29.8 172.6 -82.4 129.9 83.8 33.1 29.7 73 106 A V E + 0 0 0 -67,-3.2 -13,-2.0 -2,-0.4 -12,-0.9 0.520 57.0 28.9-122.3 -10.3 81.9 30.0 28.5 74 107 A A E -AD 6 59A 0 -68,-1.3 -68,-2.2 -15,-0.2 2,-0.3 -0.992 55.2-178.0-151.3 150.1 78.7 30.1 30.5 75 108 A N E +AD 5 58A 49 -17,-2.2 -17,-3.1 -2,-0.3 2,-0.3 -0.997 6.4 168.3-146.9 138.1 76.4 32.7 32.0 76 109 A W - 0 0 52 -72,-2.6 -19,-0.1 -2,-0.3 -20,-0.1 -0.960 44.4 -87.5-146.3 164.7 73.1 32.5 34.0 77 110 A D - 0 0 80 -2,-0.3 3,-0.3 3,-0.2 -72,-0.0 -0.589 28.7-152.9 -76.3 128.4 71.0 34.9 36.1 78 111 A P S S+ 0 0 64 0, 0.0 -1,-0.2 0, 0.0 -73,-0.0 0.797 90.7 36.1 -68.8 -24.5 72.2 35.0 39.7 79 112 A H S S+ 0 0 120 -75,-0.1 99,-3.4 98,-0.0 2,-0.3 0.354 113.5 43.8-119.8 1.4 68.8 35.9 41.1 80 113 A T E -H 177 0C 31 97,-0.3 -3,-0.2 -3,-0.3 95,-0.0 -0.980 58.3-146.0-139.0 157.1 66.1 34.1 39.0 81 114 A X E -H 176 0C 34 95,-2.5 95,-3.4 -2,-0.3 -25,-0.1 -0.995 27.7-134.4-118.7 132.4 65.6 30.6 37.6 82 115 A P E -H 175 0C 18 0, 0.0 2,-0.5 0, 0.0 93,-0.2 -0.286 7.3-141.9 -82.8 163.7 63.8 30.4 34.3 83 116 A V E -H 174 0C 0 91,-2.1 90,-3.8 -27,-0.1 91,-1.1 -0.999 33.2-162.3-115.6 120.0 61.0 28.2 33.0 84 117 A I E -H 172 0C 0 -2,-0.5 2,-0.3 88,-0.3 88,-0.2 -0.853 16.5-124.6-113.4 137.1 61.9 27.5 29.4 85 118 A T - 0 0 0 86,-2.3 85,-3.1 -2,-0.4 86,-0.2 -0.609 9.6-158.5 -78.6 141.6 59.8 26.2 26.6 86 119 A E S S+ 0 0 73 -2,-0.3 2,-0.3 83,-0.2 83,-0.2 0.444 74.0 54.2 -94.8 -2.6 61.1 23.1 24.9 87 120 A V S S- 0 0 15 81,-0.2 2,-0.2 84,-0.1 84,-0.2 -0.931 75.9-125.2-136.0 156.2 59.1 23.8 21.8 88 121 A S + 0 0 54 -2,-0.3 2,-0.3 80,-0.2 80,-0.3 -0.612 59.7 87.5 -93.4 157.4 58.6 26.5 19.2 89 122 A G E S-M 167 0D 11 78,-3.0 78,-3.4 -2,-0.2 2,-0.3 -0.965 77.0 -42.3 157.9-137.0 55.2 27.9 18.4 90 123 A F E -MN 166 133D 27 43,-3.0 43,-2.9 -2,-0.3 2,-0.3 -0.977 51.0-113.6-130.9 147.0 53.0 30.7 19.8 91 124 A V E - N 0 132D 2 74,-3.0 73,-2.4 -2,-0.3 2,-0.4 -0.549 26.9-171.6 -81.6 135.6 52.2 31.6 23.4 92 125 A R E - N 0 131D 10 39,-2.3 39,-2.7 -2,-0.3 2,-0.2 -0.972 18.9-135.1-130.4 118.3 48.7 31.2 24.8 93 126 A F E - N 0 130D 18 -2,-0.4 2,-0.4 37,-0.2 699,-0.3 -0.469 22.6-168.9 -65.6 138.4 47.7 32.6 28.2 94 127 A T E - N 0 129D 33 35,-2.0 35,-2.5 -2,-0.2 3,-0.1 -0.992 66.7 -13.1-134.6 125.4 45.7 30.2 30.2 95 128 A D S S+ 0 0 61 -2,-0.4 2,-0.8 33,-0.2 -1,-0.1 0.827 89.8 142.9 57.4 33.2 43.9 31.1 33.5 96 129 A X + 0 0 3 32,-0.1 2,-0.4 31,-0.1 -1,-0.2 -0.841 17.6 159.6-111.0 95.1 45.8 34.4 33.6 97 130 A I >>> - 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