==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=16-AUG-2013 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER RNA BINDING PROTEIN 12-FEB-13 4J6X . COMPND 2 MOLECULE: PROTEIN HFQ; . SOURCE 2 ORGANISM_SCIENTIFIC: PSEUDOMONAS AERUGINOSA; . AUTHOR A.D.NIKULIN,V.MURINA,N.LEKONTSEVA . 401 6 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 18636.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 294 73.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 161 40.1 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 6 1.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 6 1.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 3 0.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 1 0.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 45 11.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 24 6.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 54 13.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 6 1.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 6 1 11 12 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 6 A S 0 0 119 0, 0.0 375,-0.0 0, 0.0 5,-0.0 0.000 360.0 360.0 360.0 -59.2 13.4 -15.9 -5.0 2 7 A L > + 0 0 20 2,-0.1 4,-1.3 3,-0.1 5,-0.1 0.744 360.0 53.3-100.9 -38.9 11.5 -13.9 -7.6 3 8 A Q H > S+ 0 0 13 2,-0.2 4,-2.6 1,-0.2 5,-0.3 0.920 104.5 54.6 -59.8 -49.6 14.1 -14.4 -10.4 4 9 A D H > S+ 0 0 78 1,-0.2 4,-2.8 2,-0.2 -1,-0.2 0.943 111.4 39.2 -60.3 -54.3 14.2 -18.2 -10.3 5 10 A P H > S+ 0 0 47 0, 0.0 4,-2.4 0, 0.0 -1,-0.2 0.843 114.7 56.2 -67.1 -26.7 10.5 -19.1 -10.6 6 11 A Y H X S+ 0 0 2 -4,-1.3 4,-1.7 2,-0.2 -2,-0.2 0.943 111.7 41.6 -68.8 -43.7 10.1 -16.3 -13.2 7 12 A L H X S+ 0 0 0 -4,-2.6 4,-2.7 1,-0.2 -1,-0.2 0.937 113.1 56.1 -63.8 -44.6 12.8 -17.8 -15.4 8 13 A N H X S+ 0 0 50 -4,-2.8 4,-3.7 -5,-0.3 5,-0.3 0.871 104.3 51.3 -56.4 -42.4 11.4 -21.3 -14.7 9 14 A T H X S+ 0 0 15 -4,-2.4 4,-2.8 1,-0.2 6,-0.3 0.955 111.3 47.7 -61.3 -49.0 8.0 -20.4 -16.0 10 15 A L H <>S+ 0 0 0 -4,-1.7 5,-1.9 2,-0.2 -2,-0.2 0.884 117.9 44.2 -55.6 -40.4 9.5 -19.0 -19.2 11 16 A R H ><5S+ 0 0 94 -4,-2.7 3,-2.2 3,-0.2 -2,-0.2 0.996 115.4 43.9 -68.0 -64.2 11.6 -22.2 -19.3 12 17 A K H 3<5S+ 0 0 122 -4,-3.7 -2,-0.2 1,-0.3 -3,-0.2 0.837 119.1 44.6 -48.2 -39.5 8.8 -24.7 -18.5 13 18 A E T 3<5S- 0 0 69 -4,-2.8 -1,-0.3 -5,-0.3 -2,-0.2 0.292 105.2-126.4 -96.5 9.6 6.3 -23.0 -20.8 14 19 A R T < 5 + 0 0 195 -3,-2.2 -3,-0.2 1,-0.2 -4,-0.1 0.821 42.8 179.0 55.9 38.3 8.8 -22.6 -23.7 15 20 A V < - 0 0 2 -5,-1.9 16,-0.5 -6,-0.3 -1,-0.2 -0.569 28.3-123.8 -82.7 128.0 8.1 -18.8 -23.9 16 21 A P E -A 30 0A 62 0, 0.0 44,-2.2 0, 0.0 2,-0.3 -0.483 38.9-177.7 -68.4 135.7 10.0 -16.8 -26.5 17 22 A V E -AB 29 59A 4 12,-3.4 12,-1.6 42,-0.2 2,-0.5 -0.871 30.3-133.8-131.0 161.9 11.9 -13.9 -25.0 18 23 A S E -AB 28 58A 15 40,-2.5 40,-3.1 -2,-0.3 2,-0.6 -0.992 20.1-163.6-110.9 124.1 14.2 -11.0 -25.7 19 24 A I E -AB 27 57A 0 8,-3.4 8,-3.7 -2,-0.5 2,-0.5 -0.950 7.3-156.4-105.9 117.9 17.2 -10.7 -23.5 20 25 A Y E -AB 26 56A 76 36,-2.1 35,-2.6 -2,-0.6 36,-1.2 -0.825 9.7-141.9 -97.6 129.0 18.8 -7.3 -23.7 21 26 A L E > - B 0 54A 3 4,-3.8 3,-2.4 -2,-0.5 33,-0.3 -0.470 25.5-108.9 -88.1 158.9 22.5 -7.1 -22.8 22 27 A V T 3 S+ 0 0 23 31,-2.9 32,-0.1 1,-0.3 -1,-0.1 0.713 119.2 56.3 -63.1 -19.5 24.0 -4.2 -20.9 23 28 A N T 3 S- 0 0 56 30,-0.3 -1,-0.3 2,-0.1 31,-0.1 0.292 126.2 -97.7 -92.5 7.3 25.8 -3.1 -24.1 24 29 A G S < S+ 0 0 36 -3,-2.4 -2,-0.1 1,-0.3 340,-0.0 0.003 76.4 141.6 104.6 -28.1 22.5 -2.8 -26.0 25 30 A I - 0 0 88 -5,-0.1 -4,-3.8 1,-0.1 2,-0.6 -0.179 41.0-145.8 -54.3 135.1 22.6 -6.2 -27.8 26 31 A K E -A 20 0A 100 -6,-0.2 2,-0.4 -8,-0.0 -6,-0.2 -0.922 13.5-168.9-106.3 115.7 19.3 -7.9 -28.1 27 32 A L E -A 19 0A 18 -8,-3.7 -8,-3.4 -2,-0.6 2,-0.3 -0.851 6.9-160.4-102.0 142.9 19.3 -11.7 -27.9 28 33 A Q E +A 18 0A 111 -2,-0.4 2,-0.3 -10,-0.2 -10,-0.2 -0.895 34.8 71.8-122.1 152.7 16.2 -13.8 -28.8 29 34 A G E S-A 17 0A 11 -12,-1.6 -12,-3.4 -2,-0.3 2,-0.4 -0.988 72.3 -56.6 152.6-156.0 15.0 -17.3 -28.1 30 35 A Q E -AC 16 42A 64 12,-1.0 12,-2.7 -2,-0.3 2,-0.7 -0.951 49.7-107.1-123.7 144.8 13.7 -19.6 -25.4 31 36 A I E + C 0 41A 6 -16,-0.5 10,-0.2 -2,-0.4 -17,-0.2 -0.611 32.8 179.5 -74.6 110.3 15.3 -20.3 -22.1 32 37 A E E - 0 0 101 8,-2.7 2,-0.3 -2,-0.7 -1,-0.2 0.924 65.4 -36.6 -74.7 -47.2 16.6 -23.9 -22.3 33 38 A S E - C 0 40A 31 7,-1.2 7,-3.1 2,-0.0 -1,-0.3 -0.954 58.0-169.5-166.2 175.0 18.0 -23.8 -18.8 34 39 A F E - C 0 39A 41 -2,-0.3 5,-0.2 5,-0.3 2,-0.2 -0.973 3.4-167.6-164.3 160.9 19.8 -21.6 -16.2 35 40 A D - 0 0 13 3,-2.4 -2,-0.0 -2,-0.3 5,-0.0 -0.679 56.3 -61.3-136.9-154.6 21.5 -21.6 -12.9 36 41 A Q S S+ 0 0 125 -2,-0.2 3,-0.1 1,-0.2 -32,-0.0 0.799 130.8 18.8 -64.5 -29.1 22.7 -19.0 -10.4 37 42 A F S S+ 0 0 91 1,-0.1 14,-2.8 14,-0.1 15,-0.4 0.674 119.4 41.3-118.7 -25.4 25.0 -17.2 -12.9 38 43 A V E - D 0 50A 0 12,-0.2 -3,-2.4 13,-0.1 2,-0.4 -0.861 52.4-151.2-129.3 164.2 24.1 -18.1 -16.5 39 44 A I E -CD 34 49A 0 10,-2.5 10,-3.5 -2,-0.3 2,-0.7 -1.000 13.5-145.4-130.3 130.6 21.2 -18.7 -18.8 40 45 A L E -CD 33 48A 7 -7,-3.1 -8,-2.7 -2,-0.4 -7,-1.2 -0.879 20.6-169.4 -98.1 116.0 21.3 -21.0 -21.8 41 46 A L E -CD 31 47A 0 6,-2.2 6,-1.5 -2,-0.7 -10,-0.2 -0.914 5.3-166.1-113.4 123.9 19.2 -19.5 -24.6 42 47 A K E +C 30 0A 56 -12,-2.7 -12,-1.0 -2,-0.5 4,-0.1 -0.365 37.8 68.1-108.6-177.2 18.2 -21.6 -27.7 43 48 A N S S+ 0 0 114 -14,-0.2 2,-1.4 1,-0.2 -15,-0.1 0.553 98.8 25.1 71.1 139.5 16.8 -21.2 -31.3 44 49 A T S S- 0 0 150 -15,-0.1 2,-0.3 2,-0.0 -1,-0.2 -0.747 144.3 -10.6 71.9 -85.5 18.6 -19.5 -34.2 45 50 A V S S- 0 0 66 -2,-1.4 2,-0.3 -3,-0.1 83,-0.1 -0.950 78.0-125.8-127.1 153.3 21.9 -20.3 -32.4 46 51 A S + 0 0 14 -2,-0.3 2,-0.3 81,-0.2 -4,-0.2 -0.781 36.5 168.6 -95.2 148.3 22.2 -21.6 -28.9 47 52 A Q E -D 41 0A 18 -6,-1.5 -6,-2.2 -2,-0.3 2,-0.6 -0.991 37.3-119.1-158.0 146.2 24.5 -19.7 -26.5 48 53 A M E -DE 40 125A 0 77,-2.8 77,-2.8 -2,-0.3 2,-0.5 -0.838 32.9-159.8 -90.6 125.9 25.4 -19.4 -22.8 49 54 A V E -DE 39 124A 0 -10,-3.5 -10,-2.5 -2,-0.6 2,-0.3 -0.913 12.3-134.3-110.6 123.8 24.6 -16.0 -21.5 50 55 A Y E > -D 38 0A 4 73,-2.6 3,-1.0 -2,-0.5 -12,-0.2 -0.574 10.5-141.9 -70.6 135.5 26.1 -14.5 -18.3 51 56 A K G > S+ 0 0 9 -14,-2.8 3,-2.9 -2,-0.3 -1,-0.1 0.911 99.6 64.5 -64.0 -41.4 23.6 -12.9 -16.1 52 57 A H G 3 S+ 0 0 73 -15,-0.4 -1,-0.2 1,-0.3 71,-0.1 0.679 101.0 52.8 -57.4 -18.6 26.0 -10.1 -15.2 53 58 A A G < S+ 0 0 0 -3,-1.0 -31,-2.9 70,-0.2 2,-0.4 0.471 93.5 93.8 -93.9 -3.7 25.9 -9.1 -18.8 54 59 A I E < +B 21 0A 0 -3,-2.9 -33,-0.3 -33,-0.3 3,-0.1 -0.740 42.6 177.6 -95.0 134.3 22.1 -8.9 -18.9 55 60 A S E - 0 0 2 -35,-2.6 336,-3.0 -2,-0.4 2,-0.3 0.874 68.9 -3.1 -90.0 -56.5 20.0 -5.7 -18.3 56 61 A T E -BF 20 390A 4 -36,-1.2 -36,-2.1 334,-0.2 2,-0.4 -0.929 49.8-144.2-136.5 161.3 16.5 -7.0 -18.9 57 62 A V E -BF 19 389A 0 332,-2.2 332,-2.2 -2,-0.3 -38,-0.2 -0.994 20.2-170.3-123.4 122.9 14.4 -10.0 -19.9 58 63 A V E -B 18 0A 35 -40,-3.1 -40,-2.5 -2,-0.4 330,-0.1 -0.921 11.2-147.1-122.3 105.5 11.3 -9.3 -21.9 59 64 A P E -B 17 0A 5 0, 0.0 -42,-0.2 0, 0.0 327,-0.1 -0.319 17.6-129.8 -65.7 151.8 8.8 -12.1 -22.5 60 65 A S S S+ 0 0 79 -44,-2.2 -43,-0.1 1,-0.1 -2,-0.0 0.485 91.9 17.2 -80.8 -3.9 6.9 -12.1 -25.8 61 66 A R S S- 0 0 105 -45,-0.2 -1,-0.1 326,-0.0 326,-0.1 -0.976 100.6 -72.3-161.4 157.5 3.7 -12.5 -23.9 62 67 A P - 0 0 85 0, 0.0 2,-0.3 0, 0.0 324,-0.1 -0.153 48.8-154.4 -59.0 154.1 2.3 -12.1 -20.4 63 68 A V - 0 0 21 -4,-0.1 2,-0.3 -48,-0.0 324,-0.1 -0.936 25.3-104.2-134.2 143.0 3.4 -14.6 -17.8 64 69 A R - 0 0 182 -2,-0.3 -55,-0.1 1,-0.1 0, 0.0 -0.594 44.0-167.8 -65.5 132.8 2.1 -16.0 -14.5 65 70 A L 0 0 30 -2,-0.3 -1,-0.1 -56,-0.1 -56,-0.1 0.803 360.0 360.0-106.4 -33.5 4.3 -14.2 -12.0 66 71 A P 0 0 148 0, 0.0 -60,-0.1 0, 0.0 -2,-0.0 0.973 360.0 360.0 -57.4 360.0 4.2 -15.5 -8.4 67 !* 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 68 5 B H > 0 0 64 0, 0.0 4,-1.4 0, 0.0 -33,-0.1 0.000 360.0 360.0 360.0 0.3 27.0 -23.5 -11.3 69 6 B S T 4 + 0 0 107 2,-0.1 -34,-0.1 1,-0.1 -35,-0.0 0.586 360.0 31.0 -73.2 -11.2 25.2 -26.7 -12.2 70 7 B L T > S+ 0 0 9 3,-0.1 4,-2.0 2,-0.1 5,-0.2 0.770 116.4 52.5-107.3 -55.3 24.9 -25.5 -15.7 71 8 B Q H > S+ 0 0 4 1,-0.2 4,-2.5 2,-0.2 5,-0.2 0.888 108.7 49.6 -47.6 -53.3 27.9 -23.3 -16.4 72 9 B D H X S+ 0 0 62 -4,-1.4 4,-2.8 1,-0.2 -1,-0.2 0.935 114.1 40.1 -62.6 -53.8 30.6 -25.8 -15.3 73 10 B P H > S+ 0 0 36 0, 0.0 4,-2.6 0, 0.0 5,-0.2 0.852 114.0 57.6 -65.4 -27.1 29.5 -28.9 -17.2 74 11 B Y H X S+ 0 0 4 -4,-2.0 4,-1.8 2,-0.2 -2,-0.2 0.961 113.1 37.4 -64.1 -48.0 28.7 -26.6 -20.2 75 12 B L H X S+ 0 0 0 -4,-2.5 4,-2.5 -5,-0.2 -1,-0.2 0.884 115.4 55.9 -67.8 -39.3 32.3 -25.3 -20.2 76 13 B N H X S+ 0 0 49 -4,-2.8 4,-3.4 -5,-0.2 5,-0.2 0.907 107.6 47.0 -60.7 -45.6 33.7 -28.7 -19.4 77 14 B T H X S+ 0 0 9 -4,-2.6 4,-2.5 2,-0.2 6,-0.2 0.906 112.8 49.5 -63.5 -44.0 32.0 -30.4 -22.3 78 15 B L H <>S+ 0 0 0 -4,-1.8 5,-1.8 -5,-0.2 4,-0.3 0.888 114.0 48.3 -60.1 -38.8 33.2 -27.7 -24.6 79 16 B R H ><5S+ 0 0 93 -4,-2.5 3,-1.2 3,-0.2 -2,-0.2 0.971 113.9 43.2 -66.4 -54.6 36.6 -28.2 -23.1 80 17 B K H 3<5S+ 0 0 131 -4,-3.4 -2,-0.2 1,-0.3 -1,-0.2 0.844 117.5 44.9 -60.5 -37.5 36.7 -32.0 -23.5 81 18 B E T 3<5S- 0 0 97 -4,-2.5 -1,-0.3 -5,-0.2 -2,-0.2 0.316 103.8-127.2 -97.2 9.9 35.3 -32.1 -27.0 82 19 B R T < 5 - 0 0 180 -3,-1.2 -3,-0.2 -4,-0.3 -4,-0.1 0.845 40.1-178.0 51.2 41.0 37.5 -29.3 -28.2 83 20 B V < - 0 0 1 -5,-1.8 16,-0.5 -6,-0.2 -1,-0.2 -0.580 27.9-122.5 -76.6 124.1 34.5 -27.4 -29.5 84 21 B P - 0 0 60 0, 0.0 44,-2.9 0, 0.0 2,-0.3 -0.460 39.0-172.8 -60.4 135.3 35.4 -24.2 -31.3 85 22 B V E -GH 97 127A 4 12,-3.0 12,-1.6 42,-0.3 2,-0.5 -0.952 26.3-140.9-131.8 153.9 33.7 -21.3 -29.6 86 23 B S E -GH 96 126A 19 40,-2.3 40,-2.9 -2,-0.3 2,-0.6 -0.979 19.1-160.7-108.1 125.5 33.1 -17.6 -30.1 87 24 B I E -GH 95 125A 0 8,-3.0 8,-3.5 -2,-0.5 2,-0.5 -0.939 4.3-155.2-108.5 118.0 33.3 -15.6 -26.9 88 25 B Y E -GH 94 124A 84 36,-2.7 35,-2.6 -2,-0.6 36,-1.3 -0.816 11.5-142.2 -94.3 124.8 31.6 -12.1 -27.0 89 26 B L E > - H 0 122A 3 4,-3.3 3,-2.0 -2,-0.5 33,-0.3 -0.413 25.8-110.4 -79.4 161.4 32.9 -9.6 -24.5 90 27 B V T 3 S+ 0 0 37 31,-3.6 32,-0.1 1,-0.3 -1,-0.1 0.768 119.6 59.5 -60.7 -23.2 30.7 -7.1 -22.8 91 28 B N T 3 S- 0 0 54 30,-0.4 -1,-0.3 2,-0.1 31,-0.1 0.352 122.8-103.1 -88.6 5.8 32.5 -4.4 -24.9 92 29 B G S < S+ 0 0 49 -3,-2.0 2,-0.2 1,-0.3 -2,-0.1 0.290 76.9 134.4 94.1 -9.5 31.4 -6.0 -28.2 93 30 B I - 0 0 82 -5,-0.1 -4,-3.3 1,-0.0 2,-0.6 -0.500 47.6-138.5 -73.4 140.6 34.7 -7.7 -29.1 94 31 B K E -G 88 0A 97 -6,-0.2 2,-0.3 -2,-0.2 -6,-0.2 -0.903 14.8-167.6-103.3 121.4 34.6 -11.3 -30.3 95 32 B L E -G 87 0A 18 -8,-3.5 -8,-3.0 -2,-0.6 2,-0.3 -0.800 11.2-164.1 -98.1 147.5 37.2 -13.8 -29.1 96 33 B Q E +G 86 0A 104 -2,-0.3 17,-0.3 -10,-0.2 2,-0.3 -0.926 32.4 65.6-132.0 159.5 37.6 -17.2 -30.9 97 34 B G E S-G 85 0A 11 -12,-1.6 -12,-3.0 -2,-0.3 2,-0.3 -0.913 73.4 -48.7 137.3-160.0 39.2 -20.6 -30.3 98 35 B Q E -K 110 0B 61 12,-1.1 12,-3.1 -2,-0.3 2,-0.5 -0.847 47.3-111.4-116.8 151.8 38.9 -23.6 -28.0 99 36 B I E +K 109 0B 6 -16,-0.5 10,-0.2 -2,-0.3 -17,-0.2 -0.713 29.3 177.0 -83.9 122.5 38.8 -23.8 -24.2 100 37 B E E - 0 0 107 8,-3.0 2,-0.3 -2,-0.5 -1,-0.2 0.804 67.2 -30.6 -90.1 -37.4 41.9 -25.3 -22.7 101 38 B S E -K 108 0B 29 7,-1.1 7,-2.6 2,-0.0 -1,-0.4 -0.950 57.7-168.6-167.7 174.1 40.8 -24.9 -19.1 102 39 B F E -K 107 0B 40 -2,-0.3 5,-0.2 5,-0.3 2,-0.2 -0.962 2.1-166.0-166.6 157.6 38.8 -22.7 -16.8 103 40 B D - 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