==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=5-DEC-2009 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER OXIDOREDUCTASE 29-JUN-01 1JI8 . COMPND 2 MOLECULE: DISSIMILATORY SIROHEME-SULFITE REDUCTASE; . SOURCE 2 ORGANISM_SCIENTIFIC: PYROBACULUM AEROPHILUM; . AUTHOR J.R.CORT,M.A.KENNEDY,NORTHEAST STRUCTURAL GENOMICS . 111 1 2 2 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 8135.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 64 57.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 8 7.2 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 1 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 1 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 7 6.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 12 10.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 35 31.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 3 2.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 1 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 A M 0 0 172 0, 0.0 3,-0.1 0, 0.0 21,-0.0 0.000 360.0 360.0 360.0 145.1 -18.6 -8.8 6.9 2 2 A P + 0 0 98 0, 0.0 2,-0.5 0, 0.0 40,-0.0 0.871 360.0 42.7 -73.2 -38.6 -22.4 -9.0 7.2 3 3 A V S S- 0 0 2 4,-0.0 2,-1.9 2,-0.0 19,-0.1 -0.928 88.7-123.1-113.6 126.2 -22.8 -11.6 4.4 4 4 A K - 0 0 177 -2,-0.5 14,-0.1 -3,-0.1 -1,-0.0 -0.403 51.3-130.6 -65.2 87.5 -20.4 -14.6 4.2 5 5 A a S S+ 0 0 25 -2,-1.9 16,-0.2 1,-0.1 14,-0.1 -0.713 85.3 41.2 -96.3 148.5 -19.2 -13.9 0.6 6 6 A P S S+ 0 0 86 0, 0.0 2,-0.3 0, 0.0 13,-0.2 0.509 93.3 127.7 -76.0 150.5 -18.9 -15.4 -1.7 7 7 A G E -A 18 0A 18 11,-1.4 11,-1.7 12,-0.1 2,-0.3 -0.991 47.4-128.0-161.9 157.7 -22.3 -17.1 -0.9 8 8 A E E +A 17 0A 134 -2,-0.3 2,-0.3 9,-0.2 9,-0.2 -0.744 25.4 175.3-110.6 160.8 -25.6 -17.9 -2.6 9 9 A Y E -A 16 0A 73 7,-1.7 7,-2.2 -2,-0.3 2,-0.4 -0.967 23.2-125.7-154.6 169.8 -29.2 -17.1 -1.4 10 10 A Q E +A 15 0A 154 -2,-0.3 2,-0.4 5,-0.2 5,-0.2 -0.972 21.4 178.8-126.4 138.0 -32.8 -17.4 -2.4 11 11 A V - 0 0 20 3,-1.1 23,-0.1 -2,-0.4 22,-0.1 -0.949 66.8 -26.5-141.3 121.2 -35.4 -14.6 -2.5 12 12 A D S S- 0 0 117 -2,-0.4 -1,-0.1 1,-0.2 3,-0.1 0.935 129.2 -38.8 41.4 74.5 -39.1 -14.8 -3.6 13 13 A G S S+ 0 0 84 1,-0.2 2,-0.3 -3,-0.1 -1,-0.2 0.946 119.7 111.6 43.5 62.9 -38.6 -17.7 -6.0 14 14 A K - 0 0 49 2,-0.0 -3,-1.1 20,-0.0 2,-0.5 -0.994 62.2-130.9-157.7 159.9 -35.2 -16.4 -7.2 15 15 A K E -A 10 0A 145 -2,-0.3 2,-0.6 -5,-0.2 -5,-0.2 -0.943 20.0-175.4-121.9 117.1 -31.5 -17.2 -7.1 16 16 A V E -A 9 0A 1 -7,-2.2 -7,-1.7 -2,-0.5 2,-0.4 -0.925 7.0-162.7-113.5 120.4 -29.0 -14.5 -6.1 17 17 A I E -A 8 0A 85 -2,-0.6 8,-1.4 -9,-0.2 2,-0.5 -0.803 1.4-159.5-101.9 141.0 -25.2 -15.3 -6.2 18 18 A L E -AB 7 24A 2 -11,-1.7 -11,-1.4 -2,-0.4 6,-0.2 -0.954 6.2-163.7-122.4 120.3 -22.6 -13.2 -4.4 19 19 A D E >> - B 0 23A 66 4,-2.2 3,-1.3 -2,-0.5 4,-0.7 -0.113 51.4 -41.2 -86.4-169.7 -18.9 -13.3 -5.4 20 20 A E G >4 S+ 0 0 149 1,-0.3 3,-0.6 2,-0.2 -1,-0.2 -0.143 134.4 21.8 -50.7 147.5 -15.9 -12.0 -3.4 21 21 A D G 34 S- 0 0 98 1,-0.2 -1,-0.3 -16,-0.2 -2,-0.1 0.768 129.9 -79.5 64.3 20.9 -16.7 -8.7 -1.6 22 22 A a G <4 S+ 0 0 8 -3,-1.3 2,-0.4 1,-0.2 -1,-0.2 0.961 77.7 173.1 51.9 54.7 -20.4 -9.6 -1.9 23 23 A F E << -B 19 0A 59 -4,-0.7 -4,-2.2 -3,-0.6 2,-0.5 -0.765 39.1-110.2 -97.0 139.7 -20.5 -8.4 -5.6 24 24 A M E +B 18 0A 3 -2,-0.4 -6,-0.2 80,-0.2 82,-0.1 -0.510 40.4 170.5 -68.3 115.4 -23.7 -9.0 -7.7 25 25 A Q + 0 0 79 -8,-1.4 -1,-0.1 -2,-0.5 -7,-0.1 -0.068 62.5 61.7-116.9 34.4 -22.7 -11.7 -10.2 26 26 A N > + 0 0 15 -9,-0.2 3,-1.2 1,-0.0 -1,-0.2 -0.342 43.9 158.0-157.7 68.7 -26.2 -12.3 -11.6 27 27 A P G > + 0 0 54 0, 0.0 3,-0.5 0, 0.0 -1,-0.0 0.472 68.8 78.3 -73.2 -0.6 -27.8 -9.3 -13.3 28 28 A E G 3 S+ 0 0 184 1,-0.2 -2,-0.0 -3,-0.0 -3,-0.0 0.788 102.3 33.5 -79.9 -25.9 -30.1 -11.7 -15.2 29 29 A D G < S+ 0 0 67 -3,-1.2 -1,-0.2 -14,-0.1 2,-0.2 0.044 86.7 135.8-116.1 26.9 -32.4 -12.3 -12.2 30 30 A W < + 0 0 70 -3,-0.5 2,-0.2 73,-0.1 72,-0.0 -0.512 23.6 145.2 -75.6 141.3 -32.1 -8.8 -10.7 31 31 A D > - 0 0 60 -2,-0.2 4,-2.1 1,-0.1 5,-0.3 -0.839 63.5 -75.7-155.3-167.4 -35.5 -7.3 -9.5 32 32 A E H > S+ 0 0 127 -2,-0.2 4,-2.0 1,-0.2 5,-0.2 0.867 127.3 50.9 -72.5 -33.7 -37.1 -5.1 -6.8 33 33 A K H > S+ 0 0 102 2,-0.2 4,-1.4 3,-0.2 -1,-0.2 0.896 112.5 46.1 -71.5 -37.6 -36.9 -7.8 -4.2 34 34 A V H > S+ 0 0 0 2,-0.2 4,-2.3 3,-0.2 5,-0.3 0.968 115.4 44.2 -70.2 -51.2 -33.2 -8.5 -4.9 35 35 A A H X S+ 0 0 1 -4,-2.1 4,-1.9 1,-0.2 5,-0.2 0.955 118.2 44.2 -59.1 -48.5 -32.1 -4.8 -4.9 36 36 A E H X S+ 0 0 26 -4,-2.0 4,-1.5 -5,-0.3 -1,-0.2 0.820 111.1 58.3 -67.2 -27.0 -34.2 -4.1 -1.8 37 37 A W H >X S+ 0 0 42 -4,-1.4 4,-2.1 -5,-0.2 3,-0.5 0.994 109.5 39.0 -66.9 -60.8 -32.9 -7.3 -0.2 38 38 A L H 3X S+ 0 0 9 -4,-2.3 4,-1.9 1,-0.3 5,-0.3 0.905 116.1 54.1 -57.4 -39.0 -29.2 -6.5 -0.3 39 39 A A H 3X>S+ 0 0 2 -4,-1.9 5,-1.8 -5,-0.3 4,-1.2 0.852 108.7 49.3 -65.5 -30.8 -29.9 -2.9 0.6 40 40 A R H <<5S+ 0 0 133 -4,-1.5 -1,-0.2 -3,-0.5 -2,-0.2 0.867 112.2 46.6 -76.9 -34.7 -31.8 -4.1 3.7 41 41 A E H <5S+ 0 0 77 -4,-2.1 -2,-0.2 -5,-0.1 -1,-0.2 0.798 124.5 33.0 -77.3 -26.6 -29.0 -6.4 4.8 42 42 A L H <5S+ 0 0 76 -4,-1.9 -3,-0.2 -5,-0.3 -2,-0.2 0.913 137.9 15.3 -93.0 -66.1 -26.4 -3.7 4.3 43 43 A E T <5S- 0 0 61 -4,-1.2 -3,-0.2 -5,-0.3 -4,-0.1 0.837 96.2-126.0 -79.8 -31.9 -28.0 -0.3 5.1 44 44 A G < + 0 0 38 -5,-1.8 -4,-0.2 1,-0.3 -3,-0.1 0.376 49.0 161.5 102.0 -4.8 -31.0 -1.8 6.8 45 45 A I - 0 0 32 -6,-0.9 -1,-0.3 1,-0.2 3,-0.2 -0.273 19.0-173.5 -51.8 120.0 -33.5 0.0 4.6 46 46 A Q S S+ 0 0 160 1,-0.3 2,-0.4 -3,-0.1 -1,-0.2 0.767 75.7 31.0 -89.6 -26.4 -36.8 -1.9 5.1 47 47 A K S S- 0 0 161 -7,-0.0 -1,-0.3 0, 0.0 -2,-0.0 -0.864 86.3-127.2-135.5 103.6 -38.7 0.1 2.4 48 48 A M - 0 0 27 -2,-0.4 -12,-0.0 -3,-0.2 -8,-0.0 -0.221 32.1-141.6 -48.1 119.0 -36.8 1.5 -0.6 49 49 A T > - 0 0 44 1,-0.1 4,-1.0 4,-0.0 5,-0.1 0.055 26.3 -96.2 -71.6-169.1 -37.8 5.2 -0.6 50 50 A E H > S+ 0 0 143 2,-0.2 4,-1.0 1,-0.1 -1,-0.1 0.728 121.0 56.2 -84.7 -20.8 -38.4 7.1 -3.9 51 51 A E H > S+ 0 0 60 2,-0.2 4,-2.1 3,-0.1 5,-0.4 0.931 102.2 53.6 -77.0 -45.0 -34.9 8.5 -4.0 52 52 A H H > S+ 0 0 9 1,-0.2 4,-2.1 2,-0.2 5,-0.2 0.962 114.8 40.5 -54.8 -52.7 -33.1 5.1 -3.9 53 53 A W H X S+ 0 0 57 -4,-1.0 4,-1.5 2,-0.2 -1,-0.2 0.825 111.0 62.2 -67.4 -27.8 -35.1 3.8 -6.9 54 54 A K H >X S+ 0 0 118 -4,-1.0 4,-2.0 2,-0.2 3,-0.7 0.999 112.8 30.9 -61.6 -65.8 -34.7 7.2 -8.6 55 55 A L H 3X S+ 0 0 9 -4,-2.1 4,-2.2 1,-0.3 5,-0.3 0.935 117.5 58.4 -59.8 -43.4 -30.9 7.3 -8.9 56 56 A V H 3X S+ 0 0 5 -4,-2.1 4,-1.4 -5,-0.4 -1,-0.3 0.841 106.9 50.5 -56.5 -29.4 -30.9 3.5 -9.2 57 57 A K H < S+ 0 0 130 -4,-1.3 3,-1.5 -3,-0.4 -1,-0.2 0.917 107.9 49.4 -76.8 -42.4 -30.3 3.2 -18.3 62 62 A Y H >X>S+ 0 0 119 -4,-1.4 3,-1.6 1,-0.3 4,-1.0 0.818 100.5 66.4 -66.9 -26.9 -26.6 4.2 -18.7 63 63 A W T 3<5S+ 0 0 95 -4,-1.9 -1,-0.3 1,-0.3 -2,-0.2 0.559 92.8 63.5 -71.9 -2.7 -25.6 0.5 -18.1 64 64 A E T <45S+ 0 0 116 -3,-1.5 -1,-0.3 1,-0.1 -2,-0.2 0.277 98.8 53.7-103.2 11.9 -27.4 -0.3 -21.4 65 65 A T T <45S- 0 0 109 -3,-1.6 -2,-0.2 3,-0.1 -3,-0.1 0.774 135.2 -15.6-110.5 -46.7 -25.0 1.8 -23.5 66 66 A F T <5S- 0 0 183 -4,-1.0 -3,-0.1 2,-0.1 -2,-0.1 0.589 87.6-111.9-129.9 -34.1 -21.5 0.5 -22.7 67 67 A G S S+ 0 0 16 2,-0.1 4,-0.6 1,-0.1 9,-0.1 0.375 106.9 74.4-111.9 2.8 -22.3 10.6 -9.1 73 73 A K T 4 S+ 0 0 171 2,-0.1 4,-0.4 1,-0.1 -1,-0.1 0.763 98.7 45.0 -86.3 -24.9 -20.6 13.9 -10.1 74 74 A M T >4 S+ 0 0 144 2,-0.2 3,-0.7 1,-0.2 4,-0.4 0.806 104.4 61.4 -87.6 -30.4 -21.2 13.4 -13.8 75 75 A V G >4 S+ 0 0 11 -4,-0.3 3,-1.5 1,-0.2 -1,-0.2 0.838 93.8 65.6 -65.5 -29.2 -24.8 12.2 -13.5 76 76 A T G >< S+ 0 0 55 -4,-0.6 3,-1.7 1,-0.3 -1,-0.2 0.860 82.8 75.4 -62.3 -31.9 -25.7 15.6 -12.0 77 77 A K G < S+ 0 0 178 -3,-0.7 -1,-0.3 -4,-0.4 -2,-0.2 0.818 114.7 21.8 -51.0 -26.9 -24.9 17.3 -15.3 78 78 A E G < S+ 0 0 139 -3,-1.5 -1,-0.3 -4,-0.4 -2,-0.2 -0.292 130.8 37.3-137.0 52.9 -28.2 15.9 -16.5 79 79 A T S < S- 0 0 64 -3,-1.7 -3,-0.2 2,-0.1 -2,-0.1 0.108 84.5-121.0-159.4 -74.5 -30.3 15.2 -13.3 80 80 A G + 0 0 65 1,-0.2 2,-0.3 -5,-0.1 -4,-0.1 0.721 48.3 153.0 117.1 49.8 -30.1 17.6 -10.4 81 81 A F - 0 0 44 -9,-0.1 2,-0.5 -8,-0.0 -1,-0.2 -0.837 38.1-131.5-110.0 147.6 -29.0 15.6 -7.3 82 82 A S > - 0 0 72 -2,-0.3 4,-2.2 1,-0.1 5,-0.3 -0.816 4.6-145.9 -99.0 131.7 -27.1 16.9 -4.3 83 83 A L H > S+ 0 0 97 -2,-0.5 4,-1.8 1,-0.2 -1,-0.1 0.865 100.0 56.3 -62.6 -32.3 -23.9 15.1 -3.1 84 84 A E H > S+ 0 0 132 2,-0.2 4,-2.0 1,-0.2 5,-0.3 0.986 108.5 43.6 -64.5 -56.6 -24.8 16.0 0.5 85 85 A K H > S+ 0 0 118 1,-0.2 4,-1.7 2,-0.2 5,-0.2 0.957 114.3 50.6 -54.6 -50.5 -28.3 14.4 0.5 86 86 A I H X S+ 0 0 4 -4,-2.2 4,-1.9 1,-0.2 -1,-0.2 0.882 106.4 57.8 -56.9 -35.0 -27.0 11.3 -1.3 87 87 A Y H <>S+ 0 0 114 -4,-1.8 5,-0.9 -5,-0.3 3,-0.3 0.962 107.3 44.6 -61.8 -49.4 -24.2 11.1 1.3 88 88 A Q H <5S+ 0 0 165 -4,-2.0 -1,-0.2 1,-0.2 -2,-0.2 0.796 112.6 54.5 -66.2 -24.1 -26.7 10.9 4.2 89 89 A L H <5S+ 0 0 49 -4,-1.7 -1,-0.2 -5,-0.3 -2,-0.2 0.821 115.0 39.3 -79.3 -29.5 -28.7 8.4 2.1 90 90 A F T <5S- 0 0 20 -4,-1.9 2,-2.2 -3,-0.3 3,-0.0 -0.744 99.2 -94.3-116.8 167.4 -25.7 6.1 1.6 91 91 A P T 5S- 0 0 80 0, 0.0 -3,-0.1 0, 0.0 -4,-0.1 -0.457 81.6 -71.3 -78.0 70.8 -22.9 5.0 3.9 92 92 A S S + 0 0 11 -7,-0.2 4,-1.0 -6,-0.1 5,-0.2 0.213 42.3 165.3 156.8 67.0 -19.8 6.1 -0.7 94 94 A P H > S+ 0 0 12 0, 0.0 4,-1.3 0, 0.0 5,-0.4 0.618 70.8 74.2 -74.0 -12.5 -22.7 6.5 -3.2 95 95 A A H > S+ 0 0 50 2,-0.2 4,-1.4 1,-0.2 5,-0.3 0.968 112.4 14.8 -65.0 -85.2 -20.3 5.5 -6.0 96 96 A H H > S+ 0 0 120 1,-0.2 4,-1.9 2,-0.2 5,-0.2 0.801 126.0 64.7 -62.4 -24.6 -19.7 1.7 -5.6 97 97 A G H X S+ 0 0 9 -4,-1.0 4,-2.1 2,-0.2 5,-0.3 0.998 104.1 40.8 -62.8 -63.6 -22.8 1.7 -3.3 98 98 A A H X S+ 0 0 4 -4,-1.3 4,-2.1 1,-0.2 3,-0.4 0.971 115.8 49.3 -49.0 -66.2 -25.4 2.6 -5.9 99 99 A b H X>S+ 0 0 16 -4,-1.4 4,-1.8 -5,-0.4 5,-1.1 0.897 108.0 57.2 -41.9 -46.8 -23.9 0.5 -8.7 100 100 A K H ><5S+ 0 0 36 -4,-1.9 3,-0.7 -5,-0.3 -1,-0.2 0.962 108.3 44.0 -52.5 -54.9 -23.9 -2.5 -6.2 101 101 A V H 3<5S+ 0 0 13 -4,-2.1 -1,-0.3 -3,-0.4 -2,-0.2 0.811 109.1 60.4 -62.4 -26.0 -27.6 -2.2 -5.5 102 102 A A H 3<5S- 0 0 22 -4,-2.1 -1,-0.2 -5,-0.3 -2,-0.2 0.864 114.8-115.7 -71.1 -33.0 -28.2 -1.8 -9.3 103 103 A G T <<5 + 0 0 4 -4,-1.8 -3,-0.2 -3,-0.7 -2,-0.1 0.841 56.8 155.2 100.1 46.6 -26.6 -5.2 -10.0 104 104 A A < - 0 0 9 -5,-1.1 -80,-0.2 -4,-0.1 -1,-0.2 -0.925 35.4-137.7-112.4 122.8 -23.5 -4.3 -12.0 105 105 A P - 0 0 42 0, 0.0 -80,-0.1 0, 0.0 -5,-0.0 -0.374 43.5 -76.8 -72.5 151.1 -20.5 -6.7 -12.1 106 106 A K - 0 0 101 -82,-0.1 -83,-0.0 -2,-0.1 0, 0.0 -0.060 43.0-132.9 -45.1 149.9 -17.0 -5.2 -11.8 107 107 A P - 0 0 104 0, 0.0 2,-0.1 0, 0.0 -1,-0.1 0.947 64.9 -43.3 -72.7 -90.0 -15.8 -3.6 -15.0 108 108 A T + 0 0 129 2,-0.0 -2,-0.0 0, 0.0 0, 0.0 -0.559 67.3 162.8-148.6 80.2 -12.2 -4.8 -15.8 109 109 A G + 0 0 44 -2,-0.1 0, 0.0 2,-0.0 0, 0.0 0.710 23.3 123.2 -67.4-119.5 -9.9 -4.8 -12.8 110 110 A C 0 0 138 1,-0.1 -2,-0.0 0, 0.0 0, 0.0 0.908 360.0 360.0 58.9 97.7 -6.7 -6.9 -13.2 111 111 A V 0 0 205 0, 0.0 -1,-0.1 0, 0.0 -2,-0.0 0.535 360.0 360.0-141.8 360.0 -3.7 -4.6 -12.6