==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=27-SEP-2013 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER HYDROLASE 10-APR-13 4K3A . COMPND 2 MOLECULE: GLYCOSIDE HYDROLASE FAMILY 81 ENDO-BETA-1,3-GLUCA . SOURCE 2 ORGANISM_SCIENTIFIC: RHIZOMUCOR MIEHEI; . AUTHOR Z.Q.JIANG,P.ZHOU,Z.Z.CHEN,Q.J.YAN,S.Q.YANG,R.HILGENFELD . 1435 4 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 49955.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 966 67.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 14 1.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 283 19.7 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 4 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 5 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 11 0.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 4 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 125 8.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 140 9.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 329 22.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 26 1.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 6 4 6 0 2 3 3 0 2 0 0 0 2 0 4 4 0 2 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 4 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 11 6 10 12 8 4 2 3 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 2 2 2 4 0 0 2 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 5 A G 0 0 80 0, 0.0 2,-0.4 0, 0.0 277,-0.0 0.000 360.0 360.0 360.0-179.5 -13.8 37.5 1.9 2 6 A D - 0 0 87 270,-0.4 270,-1.9 569,-0.0 2,-0.2 -0.781 360.0-113.4 -98.5 132.9 -12.4 36.7 -1.5 3 7 A D B -A 271 0A 62 -2,-0.4 268,-0.2 268,-0.2 3,-0.1 -0.409 27.2-175.7 -65.2 126.0 -14.5 36.3 -4.7 4 8 A L + 0 0 2 266,-2.5 2,-1.4 -2,-0.2 267,-0.2 0.632 67.9 78.5 -99.1 -16.2 -14.5 32.7 -6.0 5 9 A F + 0 0 18 265,-0.7 -1,-0.2 506,-0.0 -2,-0.1 -0.543 69.1 119.0 -95.9 68.1 -16.5 33.3 -9.1 6 10 A V - 0 0 48 -2,-1.4 506,-0.4 -3,-0.1 507,-0.1 -0.892 69.6 -86.5-125.7 158.7 -13.8 34.8 -11.3 7 11 A P - 0 0 48 0, 0.0 3,-0.1 0, 0.0 566,-0.1 -0.297 14.1-148.1 -62.7 147.2 -12.2 33.7 -14.6 8 12 A V S S- 0 0 23 1,-0.4 2,-0.3 505,-0.1 565,-0.1 0.898 81.3 -50.8 -69.7 -48.5 -9.4 31.2 -14.6 9 13 A S - 0 0 30 494,-0.1 -1,-0.4 2,-0.1 0, 0.0 -0.959 36.9-121.7-168.7 173.6 -8.4 33.3 -17.7 10 14 A N + 0 0 70 -2,-0.3 2,-0.1 -3,-0.1 507,-0.1 -0.069 55.1 151.5-111.9 31.7 -9.8 34.6 -21.0 11 15 A F - 0 0 72 1,-0.1 507,-0.1 2,-0.1 -2,-0.1 -0.392 51.8-117.7 -62.4 132.0 -7.2 32.8 -23.1 12 16 A D > - 0 0 47 1,-0.2 3,-1.9 -2,-0.1 4,-0.2 -0.672 20.1-161.4 -77.7 109.6 -8.5 32.0 -26.6 13 17 A P G >> S+ 0 0 0 0, 0.0 4,-3.0 0, 0.0 3,-1.4 0.596 82.0 81.1 -70.4 -10.9 -8.5 28.2 -26.9 14 18 A K G 34 S+ 0 0 86 1,-0.2 5,-0.2 2,-0.2 423,-0.1 0.697 83.2 65.8 -65.6 -16.5 -8.7 28.6 -30.7 15 19 A S G <4 S+ 0 0 88 -3,-1.9 -1,-0.2 1,-0.1 -3,-0.0 0.679 116.4 23.8 -72.2 -22.0 -4.9 29.1 -30.4 16 20 A I T <4 S+ 0 0 17 -3,-1.4 -2,-0.2 1,-0.3 -1,-0.1 0.715 122.2 48.9-112.8 -40.3 -4.4 25.5 -29.2 17 21 A F S < S- 0 0 1 -4,-3.0 -1,-0.3 417,-0.0 419,-0.2 -0.919 85.9-110.8-115.2 128.2 -7.4 23.6 -30.5 18 22 A P - 0 0 65 0, 0.0 419,-2.4 0, 0.0 2,-0.2 -0.332 43.5-116.0 -51.5 132.1 -8.8 23.5 -34.1 19 23 A E E +e 437 0B 119 417,-0.2 2,-0.3 -5,-0.2 419,-0.2 -0.523 41.3 166.3 -78.4 133.9 -12.1 25.3 -34.1 20 24 A I E -e 438 0B 21 417,-3.3 419,-2.6 -2,-0.2 2,-0.5 -0.912 33.6-128.0-132.5 168.6 -15.4 23.5 -35.0 21 25 A K - 0 0 118 -2,-0.3 417,-0.1 417,-0.2 -2,-0.0 -0.992 41.8-107.1-113.4 128.9 -19.1 24.1 -34.7 22 26 A H - 0 0 1 -2,-0.5 446,-0.0 1,-0.1 428,-0.0 -0.270 19.3-143.0 -63.5 132.5 -20.9 21.3 -33.0 23 27 A P S S+ 0 0 49 0, 0.0 2,-0.9 0, 0.0 -1,-0.1 0.770 86.1 70.5 -65.0 -31.1 -23.0 19.2 -35.4 24 28 A F S S- 0 0 59 2,-0.0 -2,-0.1 513,-0.0 513,-0.0 -0.779 75.6-165.0 -96.5 102.0 -25.9 18.6 -32.9 25 29 A E - 0 0 109 -2,-0.9 2,-1.0 1,-0.1 415,-0.0 -0.445 28.2-105.9 -89.7 153.5 -27.7 21.9 -32.4 26 30 A P > - 0 0 15 0, 0.0 3,-1.3 0, 0.0 22,-0.2 -0.678 34.2-165.4 -80.4 103.8 -30.1 23.0 -29.7 27 31 A M T 3 S+ 0 0 118 -2,-1.0 21,-0.2 1,-0.3 20,-0.1 0.801 79.5 37.7 -63.9 -38.0 -33.4 23.0 -31.6 28 32 A Y T 3 S+ 0 0 59 19,-2.5 99,-3.2 98,-0.1 2,-0.4 0.195 87.6 122.4-106.3 20.1 -35.7 24.9 -29.2 29 33 A A B < -g 127 0C 13 -3,-1.3 2,-0.8 18,-0.3 99,-0.1 -0.664 67.5-124.6 -88.7 129.8 -33.2 27.5 -28.0 30 34 A N > - 0 0 42 97,-2.0 3,-0.6 -2,-0.4 5,-0.4 -0.632 31.0-170.1 -61.9 106.7 -33.7 31.2 -28.4 31 35 A T T 3 S+ 0 0 104 -2,-0.8 3,-0.3 1,-0.2 -1,-0.2 0.485 70.6 63.2 -88.0 -0.7 -30.5 31.8 -30.2 32 36 A E T 3 S+ 0 0 168 1,-0.2 -1,-0.2 2,-0.1 -2,-0.1 0.390 76.7 80.9-111.0 2.4 -30.6 35.6 -30.1 33 37 A N S < S- 0 0 49 -3,-0.6 -1,-0.2 2,-0.1 -2,-0.1 0.358 101.6-118.0 -88.9 7.0 -30.4 36.2 -26.4 34 38 A G + 0 0 73 -3,-0.3 2,-0.3 1,-0.2 -2,-0.1 0.748 67.6 138.8 68.6 24.9 -26.7 35.8 -26.5 35 39 A K - 0 0 86 -5,-0.4 2,-0.5 96,-0.0 -1,-0.2 -0.759 52.4-122.9-113.9 152.8 -26.8 32.8 -24.1 36 40 A I - 0 0 56 -2,-0.3 405,-0.1 483,-0.1 485,-0.1 -0.765 22.1-129.4 -93.4 125.0 -25.0 29.5 -24.1 37 41 A V - 0 0 10 -2,-0.5 2,-0.2 403,-0.3 483,-0.1 -0.637 27.4-130.3 -69.8 122.3 -27.0 26.3 -24.0 38 42 A P - 0 0 28 0, 0.0 3,-0.1 0, 0.0 485,-0.0 -0.516 15.6-162.3 -74.6 144.9 -25.7 24.1 -21.2 39 43 A T S S+ 0 0 1 -2,-0.2 499,-0.1 402,-0.2 484,-0.1 0.676 84.9 35.6-100.6 -24.5 -24.9 20.5 -22.0 40 44 A N S S+ 0 0 2 483,-0.3 2,-0.2 496,-0.1 493,-0.1 -0.100 93.4 119.2-124.0 34.9 -24.8 18.8 -18.6 41 45 A S S > S- 0 0 18 -3,-0.1 3,-1.5 1,-0.1 4,-0.3 -0.616 74.6-117.5-105.8 160.6 -27.6 20.7 -16.9 42 46 A W T 3 S+ 0 0 2 1,-0.3 16,-0.5 -2,-0.2 3,-0.2 0.695 118.6 50.5 -66.8 -17.4 -30.9 19.8 -15.3 43 47 A I T > S+ 0 0 1 1,-0.1 3,-1.5 89,-0.1 4,-0.4 0.487 80.9 101.1 -95.4 -0.6 -32.5 22.0 -17.9 44 48 A S G X S+ 0 0 3 -3,-1.5 3,-2.3 1,-0.3 4,-0.4 0.863 70.3 62.1 -58.3 -43.0 -30.7 20.4 -20.9 45 49 A N G > S+ 0 0 14 -4,-0.3 3,-1.5 1,-0.3 -1,-0.3 0.792 90.9 71.6 -52.7 -28.1 -33.6 18.2 -22.2 46 50 A L G < S+ 0 0 2 -3,-1.5 -1,-0.3 1,-0.3 -2,-0.2 0.775 90.7 58.2 -60.3 -25.3 -35.7 21.3 -22.8 47 51 A F G < S+ 0 0 0 -3,-2.3 -19,-2.5 -4,-0.4 -18,-0.3 0.661 106.6 48.0 -85.4 -11.1 -33.6 22.3 -25.8 48 52 A Y S < S- 0 0 11 -3,-1.5 2,-0.0 -4,-0.4 0, 0.0 -0.830 83.9-101.4-129.1 159.1 -34.1 19.1 -27.7 49 53 A P - 0 0 40 0, 0.0 491,-0.4 0, 0.0 2,-0.3 -0.345 40.5-154.0 -64.4 156.4 -36.9 16.7 -29.0 50 54 A S > - 0 0 14 490,-0.1 3,-3.0 489,-0.1 491,-0.2 -0.997 28.6 -81.5-139.8 151.6 -37.3 13.5 -27.0 51 55 A A G > S- 0 0 24 489,-2.3 3,-1.9 -2,-0.3 4,-0.1 -0.212 114.6 -14.8 -45.5 116.1 -38.6 9.9 -27.6 52 56 A D G 3 S- 0 0 134 1,-0.3 17,-0.5 2,-0.1 -1,-0.3 0.721 114.2 -80.2 54.9 25.0 -42.4 10.1 -27.3 53 57 A N G < S+ 0 0 51 -3,-3.0 13,-0.4 15,-0.1 -1,-0.3 0.806 101.6 127.2 54.1 33.3 -42.0 13.5 -25.6 54 58 A L < + 0 0 60 -3,-1.9 -2,-0.1 11,-0.1 -1,-0.1 0.472 20.4 124.7-100.5 -1.6 -41.2 11.7 -22.4 55 59 A A - 0 0 8 11,-0.1 11,-0.4 -4,-0.1 2,-0.3 -0.407 55.7-137.7 -64.0 126.8 -37.8 13.4 -21.4 56 60 A P - 0 0 0 0, 0.0 2,-0.5 0, 0.0 9,-0.2 -0.630 14.8-158.9 -89.1 145.1 -38.3 14.7 -17.9 57 61 A T B -O 64 0D 2 7,-2.8 7,-2.9 -2,-0.3 3,-0.2 -0.988 15.4-160.7-119.3 127.0 -37.1 18.1 -16.6 58 62 A T + 0 0 0 -16,-0.5 5,-0.2 -2,-0.5 262,-0.1 -0.713 29.8 161.7-122.6 82.0 -36.9 18.0 -12.8 59 63 A P - 0 0 0 0, 0.0 97,-1.7 0, 0.0 -1,-0.2 0.854 64.7 -99.4 -61.0 -32.6 -36.8 21.2 -10.9 60 64 A D S S+ 0 0 4 2,-2.3 96,-0.8 95,-0.2 236,-0.2 0.660 101.4 31.0 69.0 132.5 -37.8 19.2 -7.8 61 65 A P S S+ 0 0 0 0, 0.0 2,-0.4 0, 0.0 97,-0.1 -0.988 120.6 62.2 -82.0 6.8 -39.9 18.4 -6.0 62 66 A Y - 0 0 0 95,-0.3 -2,-2.3 18,-0.1 2,-0.5 -0.758 66.7-156.3 -91.5 137.4 -41.9 18.7 -9.3 63 67 A T E - P 0 79D 0 16,-2.9 16,-2.6 -2,-0.4 2,-0.4 -0.937 17.8-160.5-103.6 131.5 -41.0 16.5 -12.3 64 68 A L E -OP 57 78D 1 -7,-2.9 -7,-2.8 -2,-0.5 2,-0.3 -0.914 14.2-176.4-120.4 140.9 -42.1 18.0 -15.6 65 69 A R E - P 0 77D 10 12,-2.2 12,-2.6 -2,-0.4 2,-0.5 -0.958 18.7-134.9-129.3 157.6 -42.8 16.6 -19.0 66 70 A L E S- P 0 76D 10 -11,-0.4 10,-0.2 -13,-0.4 -11,-0.1 -0.943 71.7 -23.5-120.7 117.1 -43.7 18.4 -22.2 67 71 A L S S+ 0 0 10 8,-2.8 -1,-0.1 -2,-0.5 9,-0.1 0.765 84.4 157.4 60.6 29.6 -46.4 17.3 -24.7 68 72 A D + 0 0 10 7,-0.4 7,-0.3 -15,-0.1 3,-0.2 -0.358 30.2 58.5 -70.1 164.2 -46.3 13.7 -23.7 69 73 A G S S- 0 0 50 -17,-0.5 -15,-0.0 1,-0.1 -17,-0.0 -0.501 101.7 -52.3 109.3-175.1 -49.4 11.5 -24.4 70 74 A Y S S- 0 0 250 -2,-0.2 -1,-0.1 1,-0.1 -2,-0.0 0.550 81.2 -98.7 -82.1 -7.3 -51.5 10.4 -27.3 71 75 A G S S+ 0 0 30 -3,-0.2 49,-0.6 50,-0.0 50,-0.2 0.350 91.8 117.0 109.7 -3.3 -52.0 13.9 -28.6 72 76 A G S S- 0 0 28 1,-0.2 48,-2.0 47,-0.2 49,-0.2 0.959 95.2 -5.9 -60.8 -56.6 -55.5 14.5 -27.1 73 77 A N S S- 0 0 39 46,-0.2 -1,-0.2 47,-0.1 46,-0.2 -0.535 79.6-173.1-142.1 68.6 -54.6 17.4 -24.8 74 78 A P + 0 0 7 0, 0.0 41,-0.5 0, 0.0 2,-0.2 -0.306 27.6 113.9 -69.2 149.5 -50.9 18.1 -24.8 75 79 A G S S- 0 0 0 -7,-0.3 -8,-2.8 39,-0.1 2,-0.7 -0.854 72.1 -30.7-174.1-149.5 -49.4 20.6 -22.3 76 80 A L E -PQ 66 113D 0 37,-2.6 37,-2.4 -2,-0.2 2,-0.4 -0.870 59.7-152.5 -95.4 111.9 -47.0 20.7 -19.3 77 81 A T E -PQ 65 112D 3 -12,-2.6 -12,-2.2 -2,-0.7 2,-0.5 -0.710 5.8-153.8 -88.3 130.1 -47.4 17.4 -17.4 78 82 A I E +PQ 64 111D 0 33,-3.8 33,-1.7 -2,-0.4 32,-1.2 -0.882 14.2 179.0-109.2 129.9 -46.7 17.4 -13.7 79 83 A R E +P 63 0D 25 -16,-2.6 -16,-2.9 -2,-0.5 30,-0.1 -1.000 4.5 179.1-123.3 129.7 -45.6 14.4 -11.6 80 84 A Q - 0 0 0 -2,-0.4 2,-0.6 -18,-0.2 -18,-0.1 -0.856 23.3-148.0-127.5 95.4 -44.9 14.8 -7.9 81 85 A P + 0 0 0 0, 0.0 237,-0.1 0, 0.0 3,-0.1 -0.508 26.0 172.9 -77.0 112.5 -43.9 11.4 -6.7 82 86 A S S S+ 0 0 49 -2,-0.6 2,-0.3 1,-0.1 237,-0.1 0.847 74.4 29.7 -79.1 -34.0 -44.9 10.6 -3.1 83 87 A A S S- 0 0 60 123,-0.1 23,-0.4 124,-0.1 2,-0.2 -0.889 74.1-138.3-127.6 156.7 -43.8 7.0 -3.4 84 88 A K - 0 0 51 -2,-0.3 2,-0.5 21,-0.1 21,-0.2 -0.682 15.9-123.1-108.1 161.9 -41.1 5.1 -5.4 85 89 A V E -V 104 0E 68 19,-2.8 19,-2.7 -2,-0.2 2,-0.5 -0.925 27.2-162.2-104.2 124.9 -41.1 1.8 -7.3 86 90 A L E +V 103 0E 65 -2,-0.5 2,-0.3 17,-0.2 17,-0.2 -0.915 16.9 162.4-110.1 133.6 -38.4 -0.6 -6.1 87 91 A G E -V 102 0E 14 15,-1.6 14,-2.1 -2,-0.5 15,-1.6 -0.849 17.2-159.0-139.7 174.5 -37.2 -3.6 -8.1 88 92 A S - 0 0 40 12,-0.3 11,-0.1 -2,-0.3 12,-0.1 -0.906 12.5-130.9-145.4 175.4 -34.5 -6.1 -8.5 89 93 A Y - 0 0 23 -2,-0.3 9,-0.3 9,-0.3 3,-0.1 -0.947 30.9-104.8-129.3 149.7 -32.9 -8.5 -11.0 90 94 A P - 0 0 67 0, 0.0 881,-0.1 0, 0.0 7,-0.0 -0.550 50.7 -92.5 -72.9 145.1 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