==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=6-DEC-2009 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER LUMINESCENT PROTEIN 28-DEC-01 1KP5 . COMPND 2 MOLECULE: GREEN FLUORESCENT PROTEIN; . SOURCE 2 ORGANISM_SCIENTIFIC: AEQUOREA VICTORIA; . AUTHOR A.HOFMANN,H.IWAI,A.PLUECKTHUN,A.WLODAWER . 484 4 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 20961.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 338 69.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 24 5.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 190 39.3 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 4 0.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 4 0.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 4 0.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 1 0.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 44 9.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 50 10.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 15 3.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 6 1.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 2 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 2 0 0 0 4 0 2 4 4 2 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 A T 0 0 57 0, 0.0 4,-0.1 0, 0.0 204,-0.1 0.000 360.0 360.0 360.0-150.6 28.5 47.4 15.6 2 2 A G + 0 0 70 241,-0.2 3,-0.1 2,-0.1 159,-0.1 0.202 360.0 6.1 159.8 -13.4 26.9 47.1 19.0 3 3 A S S S+ 0 0 80 1,-0.3 2,-0.2 157,-0.1 158,-0.1 0.218 132.1 22.6-169.3 3.5 23.7 45.1 18.3 4 4 A R - 0 0 22 156,-0.1 158,-0.4 159,-0.0 2,-0.3 -0.812 64.9-130.4-179.6 138.2 24.1 44.1 14.6 5 5 A H - 0 0 75 -2,-0.2 159,-0.4 -4,-0.1 2,-0.2 -0.756 29.0-119.4 -98.2 140.7 26.8 43.7 12.0 6 6 A H - 0 0 31 237,-0.4 2,-0.2 -2,-0.3 3,-0.1 -0.493 8.5-148.7 -78.9 145.9 26.5 45.3 8.5 7 7 A H - 0 0 39 1,-0.4 -1,-0.1 -2,-0.2 196,-0.0 -0.359 52.8 -90.1-109.8 51.1 26.4 43.1 5.3 8 8 A H - 0 0 76 -2,-0.2 195,-0.8 195,-0.1 -1,-0.4 0.288 40.3-127.6 61.1 164.3 28.2 45.6 3.0 9 9 A H + 0 0 33 193,-0.2 192,-0.1 -3,-0.1 80,-0.1 0.162 35.6 155.8-114.9-128.5 26.4 48.2 0.8 10 10 A H + 0 0 42 190,-0.1 78,-0.0 78,-0.1 2,-0.0 0.900 45.9 99.5 96.2 61.2 26.5 48.9 -2.9 11 11 A S + 0 0 19 188,-0.1 2,-0.4 2,-0.0 191,-0.3 -0.339 35.4 159.6-176.7 86.5 23.3 50.7 -3.8 12 12 A R + 0 0 62 1,-0.1 82,-0.1 189,-0.1 76,-0.0 -0.914 34.4 90.6-119.0 141.9 23.0 54.4 -4.3 13 13 A K S >> S+ 0 0 115 -2,-0.4 3,-1.4 80,-0.1 4,-0.6 -0.105 75.4 69.0 172.1 -50.9 20.3 56.4 -6.2 14 14 A G G >4 S+ 0 0 0 82,-0.3 3,-1.6 80,-0.3 4,-0.5 0.857 92.3 70.7 -55.8 -30.8 17.5 57.2 -3.8 15 15 A E G >4 S+ 0 0 74 1,-0.3 3,-1.7 2,-0.2 -1,-0.3 0.895 91.2 56.5 -50.6 -43.5 20.2 59.4 -2.3 16 16 A E G X4 S+ 0 0 123 -3,-1.4 3,-1.1 1,-0.3 -1,-0.3 0.735 93.9 67.8 -63.9 -23.5 19.9 61.6 -5.4 17 17 A L G << S+ 0 0 61 -3,-1.6 3,-0.3 -4,-0.6 -1,-0.3 0.626 98.7 53.2 -72.4 -12.5 16.1 62.1 -4.7 18 18 A F G < S+ 0 0 5 -3,-1.7 30,-0.5 -4,-0.5 -1,-0.2 0.222 71.4 103.7-109.6 17.9 17.0 64.1 -1.5 19 19 A T S < S+ 0 0 107 -3,-1.1 2,-0.2 28,-0.1 -1,-0.2 0.686 86.2 33.4 -72.9 -17.6 19.3 66.7 -2.8 20 20 A G S S- 0 0 34 -3,-0.3 27,-0.4 -4,-0.1 2,-0.3 -0.655 108.0 -60.9-126.4-176.1 16.7 69.4 -2.7 21 21 A V - 0 0 77 -2,-0.2 25,-0.2 25,-0.1 -2,-0.1 -0.522 55.2-160.5 -66.9 128.1 13.6 70.4 -0.6 22 22 A V E -A 45 0A 10 23,-3.0 23,-1.5 -2,-0.3 2,-0.1 -0.962 15.5-122.5-119.2 127.9 11.1 67.6 -0.9 23 23 A P E -A 44 0A 57 0, 0.0 104,-2.6 0, 0.0 2,-0.4 -0.422 27.6-150.1 -68.0 135.8 7.4 67.9 -0.1 24 24 A I E -Ab 43 127A 4 19,-2.9 19,-1.7 102,-0.2 2,-0.4 -0.898 13.3-171.4-112.6 139.8 6.1 65.6 2.6 25 25 A L E -Ab 42 128A 1 102,-2.3 104,-2.4 -2,-0.4 2,-0.4 -0.995 5.5-169.4-128.7 125.5 2.6 64.1 3.0 26 26 A V E +Ab 41 129A 0 15,-3.5 15,-2.8 -2,-0.4 2,-0.4 -0.978 6.2 178.0-119.9 130.7 1.6 62.2 6.2 27 27 A E E -Ab 40 130A 1 102,-2.4 104,-3.0 -2,-0.4 2,-0.4 -0.985 4.7-178.6-133.0 123.0 -1.6 60.2 6.5 28 28 A L E -Ab 39 131A 0 11,-2.8 11,-2.1 -2,-0.4 2,-0.5 -0.957 12.4-168.7-125.8 136.3 -2.5 58.2 9.6 29 29 A D E +Ab 38 132A 33 102,-2.1 104,-3.1 -2,-0.4 2,-0.3 -0.989 24.9 179.0-122.3 119.2 -5.5 56.0 10.4 30 30 A G E -Ab 37 133A 2 7,-2.7 7,-2.5 -2,-0.5 2,-0.3 -0.890 29.9-173.7-131.0 160.3 -5.8 55.1 14.0 31 31 A D E +Ab 36 134A 33 102,-1.7 104,-2.7 -2,-0.3 2,-0.5 -0.930 13.3 178.4-145.8 110.6 -7.9 53.2 16.6 32 32 A V E > S-Ab 35 135A 0 3,-2.0 3,-2.6 -2,-0.3 104,-0.2 -0.973 75.6 -23.7-120.4 115.7 -6.9 53.6 20.3 33 33 A N T 3 S- 0 0 55 102,-2.4 -1,-0.1 -2,-0.5 103,-0.1 0.900 128.4 -49.2 52.2 43.7 -9.1 51.7 22.8 34 34 A G T 3 S+ 0 0 54 101,-0.3 2,-1.1 1,-0.2 -1,-0.3 0.345 110.7 126.2 80.5 -9.5 -12.0 51.8 20.3 35 35 A H E < -A 32 0A 77 -3,-2.6 -3,-2.0 332,-0.1 2,-0.2 -0.724 47.1-159.3 -87.1 100.7 -11.5 55.5 19.7 36 36 A K E +A 31 0A 75 -2,-1.1 2,-0.3 -5,-0.2 -5,-0.2 -0.550 22.7 149.8 -80.8 144.7 -11.2 55.8 15.9 37 37 A F E -A 30 0A 1 -7,-2.5 -7,-2.7 -2,-0.2 2,-0.3 -0.984 30.0-139.3-164.1 168.2 -9.6 58.8 14.3 38 38 A S E -A 29 0A 0 -2,-0.3 22,-0.8 -9,-0.2 21,-0.7 -0.963 9.9-164.3-138.2 152.6 -7.6 60.2 11.4 39 39 A V E -AC 28 58A 0 -11,-2.1 -11,-2.8 -2,-0.3 2,-0.4 -1.000 1.3-166.1-140.7 140.5 -4.7 62.6 11.0 40 40 A S E +AC 27 57A 14 17,-2.3 17,-3.0 -2,-0.4 2,-0.3 -0.976 10.5 179.3-126.0 137.8 -3.3 64.4 8.0 41 41 A G E +AC 26 56A 1 -15,-2.8 -15,-3.5 -2,-0.4 2,-0.3 -0.990 3.9 179.3-139.2 148.5 0.0 66.2 7.8 42 42 A E E +AC 25 55A 71 13,-1.8 13,-2.4 -2,-0.3 2,-0.3 -0.962 22.2 107.6-144.6 156.9 2.0 68.1 5.2 43 43 A G E -AC 24 54A 25 -19,-1.7 -19,-2.9 -2,-0.3 2,-0.3 -0.985 52.4 -68.9 170.1-156.2 5.3 70.0 5.0 44 44 A E E -AC 23 53A 108 9,-2.1 9,-2.6 -2,-0.3 2,-0.3 -0.919 24.9-164.3-131.9 158.4 8.8 70.0 3.7 45 45 A G E -AC 22 52A 2 -23,-1.5 -23,-3.0 -2,-0.3 2,-0.4 -0.998 4.7-171.6-143.5 135.9 12.0 68.1 4.3 46 46 A D E >>> - C 0 51A 31 5,-3.8 3,-2.2 -2,-0.3 5,-2.0 -0.794 0.3-175.1-131.2 89.9 15.6 69.0 3.3 47 47 A A T 345S+ 0 0 6 -2,-0.4 3,-0.5 -27,-0.4 35,-0.1 0.774 78.1 75.8 -53.7 -32.0 17.9 66.1 4.1 48 48 A T T 345S+ 0 0 65 -30,-0.5 -1,-0.3 1,-0.3 -29,-0.1 0.802 116.4 20.1 -52.4 -27.8 20.9 68.2 3.0 49 49 A Y T <45S- 0 0 149 -3,-2.2 -1,-0.3 2,-0.1 -2,-0.2 0.310 108.3-116.8-122.5 3.7 20.5 69.9 6.4 50 50 A G T <5 + 0 0 1 -4,-0.6 182,-2.7 -3,-0.5 2,-0.4 0.794 67.8 146.3 62.7 29.0 18.5 67.2 8.1 51 51 A K E < +CD 46 231A 93 -5,-2.0 -5,-3.8 180,-0.2 2,-0.3 -0.804 22.2 168.2-105.9 137.6 15.6 69.7 8.4 52 52 A L E -CD 45 230A 8 178,-2.2 178,-2.3 -2,-0.4 2,-0.4 -0.999 13.9-167.8-147.6 140.8 11.9 69.1 8.2 53 53 A T E +CD 44 229A 51 -9,-2.6 -9,-2.1 -2,-0.3 2,-0.3 -0.998 17.1 171.1-129.1 127.3 8.8 71.1 9.0 54 54 A L E -CD 43 228A 7 174,-2.5 174,-3.6 -2,-0.4 2,-0.4 -0.980 21.9-156.0-139.8 148.6 5.3 69.5 9.2 55 55 A K E -CD 42 227A 66 -13,-2.4 -13,-1.8 -2,-0.3 2,-0.4 -0.995 16.6-162.0-123.9 128.3 1.8 70.6 10.3 56 56 A F E -CD 41 226A 0 170,-3.1 170,-1.9 -2,-0.4 2,-0.4 -0.896 5.4-169.5-114.4 145.2 -0.6 67.9 11.4 57 57 A I E -CD 40 225A 20 -17,-3.0 -17,-2.3 -2,-0.4 2,-1.0 -0.995 22.9-136.1-135.6 127.6 -4.4 68.1 11.7 58 58 A C E > -C 39 0A 2 166,-2.5 3,-0.8 -2,-0.4 166,-0.4 -0.749 22.8-170.9 -81.3 106.1 -6.8 65.7 13.3 59 59 A T T 3 S+ 0 0 30 -2,-1.0 -1,-0.2 -21,-0.7 -20,-0.1 0.496 75.3 59.8 -79.1 -4.3 -9.5 65.6 10.6 60 60 A T T 3 S- 0 0 13 -22,-0.8 309,-0.6 1,-0.2 2,-0.4 0.389 116.7 -93.8-105.6 5.0 -12.0 63.6 12.6 61 61 A G S < S+ 0 0 44 -3,-0.8 163,-0.5 -23,-0.2 2,-0.4 -0.977 89.1 16.6 128.8-124.4 -12.4 66.1 15.5 62 62 A K S S- 0 0 124 -2,-0.4 -3,-0.0 -3,-0.1 161,-0.0 -0.774 79.1-114.1 -92.5 130.1 -10.4 66.0 18.8 63 63 A L - 0 0 1 -2,-0.4 4,-0.1 1,-0.2 -26,-0.0 -0.444 19.7-146.5 -62.3 128.2 -7.3 63.8 18.8 64 64 A P S S+ 0 0 25 0, 0.0 -1,-0.2 0, 0.0 83,-0.1 0.644 80.5 42.1 -71.1 -17.6 -8.0 60.9 21.3 65 65 A V S S- 0 0 0 81,-0.1 80,-0.1 82,-0.1 2,-0.1 -0.868 103.0 -81.0-127.2 162.6 -4.3 60.9 22.4 66 66 A P > - 0 0 6 0, 0.0 3,-1.0 0, 0.0 4,-0.3 -0.413 37.0-126.8 -65.0 135.6 -1.7 63.6 23.2 67 67 A W G > S+ 0 0 12 1,-0.2 3,-1.5 2,-0.2 4,-0.5 0.832 105.5 61.4 -50.2 -39.2 -0.1 65.0 20.1 68 68 A P G > S+ 0 0 4 0, 0.0 3,-1.2 0, 0.0 4,-0.4 0.865 93.5 62.1 -59.9 -36.8 3.4 64.3 21.5 69 69 A T G < S+ 0 0 3 -3,-1.0 -2,-0.2 1,-0.2 4,-0.2 0.568 104.6 50.2 -67.4 -6.4 2.9 60.5 21.7 70 70 A L G <> S+ 0 0 0 -3,-1.5 4,-2.7 -4,-0.3 3,-0.4 0.513 81.3 94.7-107.7 -8.1 2.4 60.5 17.9 71 71 A V T <4 S+ 0 0 7 -3,-1.2 -2,-0.1 -4,-0.5 -1,-0.1 0.924 86.6 46.4 -48.7 -56.9 5.5 62.5 16.9 72 72 A T T 4 S+ 0 0 27 -4,-0.4 -1,-0.2 1,-0.2 -2,-0.1 0.814 113.3 50.4 -59.8 -32.6 7.7 59.4 16.3 73 73 A T T 4 0 0 6 -3,-0.4 -1,-0.2 -4,-0.2 -2,-0.2 0.945 360.0 360.0 -70.3 -48.0 5.0 57.8 14.3 74 74 A F < 0 0 8 -4,-2.7 -2,-0.2 -48,-0.1 -3,-0.2 0.923 360.0 360.0 -62.0 360.0 4.4 60.8 12.0 75 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 76 78 A V > 0 0 20 0, 0.0 3,-2.2 0, 0.0 4,-0.1 0.000 360.0 360.0 360.0 55.4 10.5 59.9 7.7 77 79 A Q G > + 0 0 25 1,-0.3 3,-1.6 2,-0.2 16,-0.2 0.513 360.0 87.4 -59.4 -4.0 14.1 59.3 8.9 78 80 A C G 3 S+ 0 0 0 1,-0.3 -1,-0.3 14,-0.2 -31,-0.1 0.636 81.2 63.0 -71.0 -10.8 15.0 59.3 5.2 79 81 A F G < S+ 0 0 2 -3,-2.2 -1,-0.3 -29,-0.1 153,-0.3 0.397 75.9 119.7 -93.6 3.6 15.4 63.1 5.8 80 82 A S S < S- 0 0 0 -3,-1.6 2,-0.7 1,-0.1 153,-0.2 -0.290 72.6-112.7 -65.0 151.1 18.2 62.7 8.3 81 83 A R B -e 233 0A 99 151,-2.7 153,-3.1 -32,-0.2 -33,-0.1 -0.815 30.9-164.7 -90.1 116.2 21.4 64.4 7.4 82 84 A Y - 0 0 4 -2,-0.7 5,-0.1 151,-0.2 153,-0.1 -0.911 18.8-129.3-100.3 121.5 24.0 61.7 6.8 83 85 A P >> - 0 0 18 0, 0.0 3,-2.5 0, 0.0 4,-0.6 -0.265 28.5-102.0 -64.7 159.5 27.6 63.2 6.7 84 86 A D G >4 S+ 0 0 146 1,-0.3 3,-1.5 2,-0.2 4,-0.3 0.888 122.7 55.0 -48.6 -47.2 29.8 62.3 3.8 85 87 A H G 34 S+ 0 0 79 1,-0.3 155,-0.3 2,-0.1 -1,-0.3 0.474 111.5 46.6 -70.4 2.5 31.7 59.7 5.8 86 88 A M G X4 S+ 0 0 0 -3,-2.5 3,-2.6 1,-0.1 -1,-0.3 0.359 75.6 107.6-121.9 2.6 28.4 58.0 6.6 87 89 A K G X< S+ 0 0 61 -3,-1.5 3,-1.0 -4,-0.6 -2,-0.1 0.767 74.5 60.5 -53.3 -30.1 26.9 57.9 3.1 88 90 A R G 3 S+ 0 0 135 -4,-0.3 -1,-0.3 1,-0.2 115,-0.1 0.383 102.8 55.6 -80.2 7.0 27.4 54.2 2.8 89 91 A H G < S+ 0 0 17 -3,-2.6 2,-1.1 115,-0.1 -1,-0.2 0.193 71.3 113.2-124.4 17.5 25.2 53.7 5.9 90 92 A D <> + 0 0 6 -3,-1.0 4,-1.4 1,-0.2 112,-0.3 -0.696 29.8 165.8 -95.7 87.6 22.0 55.5 4.9 91 93 A F H > + 0 0 1 -2,-1.1 4,-1.5 1,-0.2 111,-0.2 0.936 70.0 60.7 -63.0 -52.8 19.4 52.7 4.6 92 94 A F H 4 S+ 0 0 0 1,-0.2 3,-0.3 2,-0.2 4,-0.2 0.876 111.2 37.6 -44.8 -50.5 16.4 55.0 4.5 93 95 A K H >4 S+ 0 0 5 1,-0.2 3,-1.3 2,-0.2 -1,-0.2 0.827 109.0 62.8 -76.2 -29.8 17.4 56.9 1.3 94 96 A S H 3< S+ 0 0 12 -4,-1.4 -80,-0.3 1,-0.3 -1,-0.2 0.787 98.2 57.6 -65.6 -26.2 18.8 53.8 -0.4 95 97 A A T 3X S+ 0 0 4 -4,-1.5 4,-2.6 -3,-0.3 102,-0.3 0.541 93.2 141.7 -81.5 -8.5 15.4 52.1 -0.4 96 98 A M T <4 + 0 0 4 -3,-1.3 -82,-0.3 2,-0.3 -81,-0.1 -0.482 48.5 21.4 -80.9 156.2 13.8 55.0 -2.3 97 99 A P T 4 S+ 0 0 56 0, 0.0 25,-1.8 0, 0.0 -1,-0.2 -0.984 130.4 43.8 -80.0 -18.3 11.8 55.5 -4.4 98 100 A E T 4 S- 0 0 96 23,-0.2 -2,-0.3 1,-0.1 2,-0.1 0.891 101.8-164.5 -51.0 -41.3 10.1 52.2 -3.8 99 101 A G < - 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