==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=6-SEP-2013 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER HYDROLASE 25-JUN-13 4LE4 . COMPND 2 MOLECULE: BETA-GLUCANASE; . SOURCE 2 ORGANISM_SCIENTIFIC: PODOSPORA ANSERINA; . AUTHOR T.JIANG,H.C.CHAN,C.H.HUANG,T.P.KO,T.Y.HUANG,J.R.LIU,R.T.GUO . 982 4 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 37396.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 636 64.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 367 37.4 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 12 1.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 8 0.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 128 13.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 104 10.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 8 0.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 4 0.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 7 0 7 9 20 4 4 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 19 A G 0 0 73 0, 0.0 2,-0.3 0, 0.0 233,-0.2 0.000 360.0 360.0 360.0 167.4 28.1 16.8 50.7 2 20 A T E -A 233 0A 82 231,-1.1 231,-2.2 233,-0.4 2,-0.6 -0.905 360.0-134.2-152.9 121.5 31.2 18.2 52.5 3 21 A I E -A 232 0A 61 -2,-0.3 229,-0.3 229,-0.2 3,-0.2 -0.676 21.1-179.6 -79.4 118.3 32.1 18.0 56.2 4 22 A L E S+ 0 0 48 227,-2.4 2,-0.3 -2,-0.6 84,-0.2 0.823 73.1 5.8 -83.8 -36.9 33.1 21.4 57.4 5 23 A W E -A 231 0A 13 226,-1.8 226,-2.8 81,-0.1 2,-0.6 -0.983 66.1-148.4-151.7 136.3 33.9 20.3 60.9 6 24 A D E +A 230 0A 61 -2,-0.3 3,-0.2 224,-0.2 224,-0.2 -0.924 20.3 167.6-111.0 116.6 34.0 16.9 62.5 7 25 A G + 0 0 0 222,-1.8 222,-0.3 -2,-0.6 223,-0.2 0.057 45.2 110.8-115.1 29.3 33.1 16.6 66.2 8 26 A R S S- 0 0 17 220,-0.1 52,-0.5 49,-0.1 51,-0.3 0.448 80.7-126.7 -83.6 0.9 32.8 12.9 66.4 9 27 A F > + 0 0 11 -3,-0.2 3,-2.5 220,-0.1 -1,-0.1 0.492 61.2 143.5 68.7 12.2 35.9 12.5 68.5 10 28 A N T 3 + 0 0 49 1,-0.3 -1,-0.1 47,-0.1 -3,-0.0 0.701 69.3 47.3 -56.8 -25.0 37.4 10.0 66.1 11 29 A D T 3 S+ 0 0 167 2,-0.0 2,-0.4 0, 0.0 -1,-0.3 0.292 97.7 90.5 -98.2 8.3 41.0 11.2 66.5 12 30 A M < - 0 0 11 -3,-2.5 3,-0.1 1,-0.1 6,-0.0 -0.837 56.7-161.9-104.8 144.3 40.8 11.2 70.3 13 31 A T S S- 0 0 112 -2,-0.4 2,-0.3 1,-0.3 -1,-0.1 0.865 77.0 -14.0 -86.7 -38.1 41.7 8.3 72.5 14 32 A S S > S- 0 0 48 31,-0.0 3,-1.8 33,-0.0 -1,-0.3 -0.917 77.7 -89.8-154.5 171.8 39.8 9.5 75.6 15 33 A S G > S+ 0 0 8 1,-0.3 3,-2.0 -2,-0.3 4,-0.3 0.644 111.4 82.1 -66.9 -12.7 38.2 12.8 76.9 16 34 A A G > S+ 0 0 39 1,-0.3 3,-1.7 2,-0.2 -1,-0.3 0.769 76.4 72.3 -60.7 -25.0 41.6 13.7 78.4 17 35 A D G X S+ 0 0 35 -3,-1.8 3,-1.4 1,-0.3 -1,-0.3 0.700 81.1 71.5 -65.9 -15.9 42.5 14.9 74.9 18 36 A L G X S+ 0 0 5 -3,-2.0 3,-1.4 1,-0.3 -1,-0.3 0.785 85.4 67.6 -68.4 -26.6 40.1 17.8 75.5 19 37 A N G < S+ 0 0 47 -3,-1.7 -1,-0.3 -4,-0.3 -2,-0.2 0.599 81.4 76.1 -68.6 -11.8 42.7 19.2 78.0 20 38 A K G < S+ 0 0 136 -3,-1.4 7,-0.3 -4,-0.2 -1,-0.3 0.751 81.1 86.8 -71.4 -19.1 45.0 19.8 75.1 21 39 A W < + 0 0 17 -3,-1.4 2,-0.3 -4,-0.2 5,-0.2 -0.580 51.0 155.2 -84.2 142.6 43.0 22.9 74.2 22 40 A S B > -H 25 0B 52 3,-1.3 3,-1.1 -2,-0.2 5,-0.1 -0.963 55.6-104.2-156.3 160.6 43.6 26.3 75.7 23 41 A W T 3 S+ 0 0 145 -2,-0.3 3,-0.4 1,-0.3 -1,-0.1 0.899 124.2 46.2 -57.1 -38.8 43.0 29.9 74.8 24 42 A G T 3 S+ 0 0 78 1,-0.3 -1,-0.3 -3,-0.1 2,-0.1 0.577 125.1 30.7 -81.0 -9.6 46.7 30.2 74.0 25 43 A N B < S-H 22 0B 93 -3,-1.1 2,-2.2 2,-0.1 -3,-1.3 -0.626 71.4-173.1-149.3 79.5 46.8 27.0 72.0 26 44 A Q + 0 0 84 -3,-0.4 2,-0.2 -5,-0.2 -5,-0.1 -0.387 29.5 151.9 -77.7 65.5 43.5 26.4 70.4 27 45 A V + 0 0 59 -2,-2.2 3,-0.3 -7,-0.3 -2,-0.1 -0.549 33.3 28.4 -96.9 161.9 44.2 22.9 69.0 28 46 A G S S- 0 0 54 -2,-0.2 -10,-0.0 1,-0.2 3,-0.0 -0.225 97.8 -65.4 85.7-178.4 41.9 20.0 68.3 29 47 A P S S+ 0 0 41 0, 0.0 56,-2.5 0, 0.0 2,-0.4 0.574 108.5 52.0 -85.1 -12.1 38.2 19.9 67.3 30 48 A Y E -I 84 0C 3 -3,-0.3 2,-0.5 54,-0.3 -9,-0.1 -0.985 66.2-137.5-135.8 137.9 36.7 21.4 70.5 31 49 A Q E -I 83 0C 13 52,-3.7 52,-1.7 -2,-0.4 2,-0.6 -0.738 25.7-163.8 -84.2 131.6 37.1 24.4 72.8 32 50 A Y E +I 82 0C 14 -2,-0.5 50,-0.1 -14,-0.2 3,-0.1 -0.897 49.4 99.6-123.2 99.5 36.9 23.1 76.4 33 51 A Y E + 0 0 36 -2,-0.6 2,-1.4 48,-0.5 -1,-0.1 0.460 51.1 90.1-150.1 -19.4 36.2 25.8 79.0 34 52 A I E S+I 81 0C 3 47,-0.6 47,-2.4 -3,-0.2 2,-0.3 -0.677 70.2 97.1 -89.7 87.4 32.5 25.9 79.9 35 53 A H S S- 0 0 46 -2,-1.4 38,-0.3 45,-0.2 45,-0.1 -0.955 71.1 -93.4-160.3 169.8 32.6 23.4 82.8 36 54 A G - 0 0 5 -2,-0.3 34,-0.2 43,-0.2 36,-0.1 0.078 52.3 -77.3 -80.7-164.0 32.8 23.4 86.5 37 55 A S S S+ 0 0 97 32,-1.0 33,-0.1 34,-0.2 -1,-0.1 0.564 98.0 89.7 -76.8 -12.2 35.7 23.1 88.9 38 56 A S S S- 0 0 16 31,-0.3 705,-0.1 1,-0.1 703,-0.0 -0.294 87.0 -87.2 -82.7 170.4 36.4 19.4 88.6 39 57 A P > - 0 0 18 0, 0.0 3,-2.1 0, 0.0 4,-0.5 -0.286 47.2 -98.4 -70.0 163.2 38.7 17.6 86.1 40 58 A V G >> S+ 0 0 9 701,-0.4 4,-2.8 1,-0.3 3,-2.2 0.847 118.2 67.2 -56.2 -37.2 37.3 16.6 82.7 41 59 A S G 34 S+ 0 0 16 1,-0.3 -1,-0.3 701,-0.3 701,-0.1 0.659 93.3 61.7 -62.5 -10.6 36.6 13.0 83.6 42 60 A A G <4 S+ 0 0 27 -3,-2.1 -1,-0.3 1,-0.1 -2,-0.2 0.762 120.4 22.7 -79.5 -24.4 33.9 14.3 86.0 43 61 A Y T <4 S+ 0 0 0 -3,-2.2 22,-2.8 -4,-0.5 2,-0.4 0.703 124.1 34.2-115.0 -28.1 32.0 15.8 83.0 44 62 A V E < +B 64 0A 0 -4,-2.8 2,-0.3 20,-0.2 -1,-0.3 -0.993 55.8 168.0-138.1 128.8 32.9 14.1 79.7 45 63 A N E -B 63 0A 21 18,-1.9 18,-3.5 -2,-0.4 2,-0.3 -0.998 20.3-144.8-140.5 137.9 33.8 10.5 79.1 46 64 A L E +B 62 0A 23 -2,-0.3 16,-0.2 16,-0.2 -31,-0.1 -0.800 28.2 148.5-106.3 146.3 34.1 8.6 75.8 47 65 A S E > -B 61 0A 19 14,-2.0 14,-3.1 -2,-0.3 3,-1.7 -0.946 53.8-122.1-169.0 148.3 33.2 5.0 75.0 48 66 A P G > S+ 0 0 71 0, 0.0 3,-0.9 0, 0.0 14,-0.1 0.745 114.9 63.6 -65.5 -19.6 31.9 2.9 72.2 49 67 A D G 3 S+ 0 0 127 1,-0.2 11,-0.1 12,-0.1 13,-0.1 0.585 93.9 61.5 -80.6 -7.3 29.0 2.1 74.6 50 68 A Y G < S+ 0 0 51 -3,-1.7 12,-0.4 11,-0.2 -1,-0.2 0.251 91.0 79.5-103.4 10.0 27.9 5.7 74.6 51 69 A K S < S- 0 0 37 -3,-0.9 10,-0.1 10,-0.3 176,-0.1 -0.674 94.0 -85.3-113.0 169.0 27.0 6.2 70.9 52 70 A N > - 0 0 4 -2,-0.2 3,-1.8 1,-0.2 6,-0.2 -0.632 40.2-141.2 -67.4 120.6 24.1 5.4 68.7 53 71 A P T 3 S+ 0 0 100 0, 0.0 -1,-0.2 0, 0.0 131,-0.0 0.767 98.7 58.8 -58.7 -24.3 25.0 1.8 67.7 54 72 A A T 3 S+ 0 0 14 1,-0.1 2,-1.5 129,-0.1 3,-0.1 0.660 83.4 90.5 -77.9 -18.1 23.8 2.4 64.1 55 73 A D X + 0 0 1 -3,-1.8 3,-1.3 1,-0.2 186,-0.2 -0.609 45.9 168.8 -82.6 87.9 26.3 5.2 63.6 56 74 A T T 3 S+ 0 0 73 -2,-1.5 -1,-0.2 1,-0.3 185,-0.1 0.542 70.6 72.3 -77.5 -3.1 29.3 3.4 62.2 57 75 A G T 3 S+ 0 0 9 183,-0.2 2,-0.5 -3,-0.1 -1,-0.3 0.634 91.0 63.0 -84.7 -12.7 30.8 6.8 61.4 58 76 A S < - 0 0 0 -3,-1.3 3,-0.1 -6,-0.2 -49,-0.1 -0.951 62.3-166.4-114.8 125.3 31.5 7.5 65.1 59 77 A R S S+ 0 0 115 -2,-0.5 2,-0.3 -51,-0.3 -1,-0.1 0.906 76.8 13.3 -77.0 -40.5 33.9 5.3 67.0 60 78 A Q - 0 0 20 -52,-0.5 2,-0.2 -10,-0.1 -1,-0.2 -0.942 67.8-144.4-133.5 154.6 33.0 6.4 70.5 61 79 A G E -B 47 0A 0 -14,-3.1 -14,-2.0 -2,-0.3 2,-0.5 -0.632 23.6-119.7-107.9 172.5 30.1 8.5 71.9 62 80 A A E -BC 46 226A 0 164,-2.9 164,-2.2 -12,-0.4 2,-0.7 -0.949 7.1-149.1-119.9 131.7 30.5 10.9 74.8 63 81 A K E -BC 45 225A 41 -18,-3.5 -18,-1.9 -2,-0.5 2,-0.5 -0.895 19.4-161.9 -94.1 120.8 28.7 10.8 78.1 64 82 A I E -BC 44 224A 1 160,-3.4 160,-2.7 -2,-0.7 2,-0.4 -0.927 11.0-162.7-106.0 126.7 28.3 14.4 79.2 65 83 A T E - C 0 223A 8 -22,-2.8 2,-0.5 -2,-0.5 158,-0.2 -0.912 21.2-161.3-119.5 138.9 27.6 14.8 82.9 66 84 A L E + C 0 222A 0 156,-2.5 156,-2.2 -2,-0.4 2,-0.2 -0.965 29.3 162.3-110.4 126.6 26.2 17.5 85.2 67 85 A D > - 0 0 25 -2,-0.5 3,-1.5 154,-0.2 153,-0.1 -0.618 57.8 -71.7-128.9-167.0 27.1 16.9 88.8 68 86 A N T 3 S+ 0 0 71 1,-0.3 151,-0.1 -2,-0.2 -2,-0.0 0.674 131.1 46.7 -65.7 -13.7 27.2 19.0 91.9 69 87 A T T 3 S+ 0 0 18 1,-0.1 2,-2.4 -27,-0.1 -32,-1.0 0.355 74.9 114.9-107.0 3.5 30.2 20.9 90.7 70 88 A A < + 0 0 0 -3,-1.5 9,-2.3 -34,-0.2 2,-0.4 -0.266 42.5 122.7 -75.2 56.7 29.1 21.7 87.2 71 89 A Y B -J 78 0C 70 -2,-2.4 2,-0.4 7,-0.2 3,-0.2 -0.976 45.1-156.8-122.0 127.8 28.9 25.4 87.6 72 90 A W > - 0 0 66 5,-0.5 3,-1.9 -2,-0.4 5,-0.2 -0.862 65.5 -9.7-109.3 137.4 30.9 27.8 85.3 73 91 A N T 3 S- 0 0 118 -2,-0.4 -1,-0.2 -38,-0.3 -37,-0.1 0.846 121.3 -60.5 53.2 41.4 32.0 31.3 86.0 74 92 A G T 3 S+ 0 0 85 -3,-0.2 -1,-0.3 1,-0.2 2,-0.1 0.638 95.9 146.8 65.1 14.2 30.0 31.8 89.2 75 93 A Q < - 0 0 27 -3,-1.9 -1,-0.2 2,-0.1 389,-0.0 -0.406 52.2-135.2 -81.4 162.3 26.6 31.2 87.4 76 94 A N S S+ 0 0 87 -2,-0.1 2,-0.4 -5,-0.1 -1,-0.1 0.316 81.6 84.8 -99.2 4.4 23.6 29.5 89.1 77 95 A M S S- 0 0 16 -5,-0.2 -5,-0.5 135,-0.1 2,-0.3 -0.840 82.2-122.0-101.0 144.5 22.8 27.3 86.0 78 96 A R E +JK 71 211C 0 133,-2.1 133,-2.7 -2,-0.4 2,-0.3 -0.721 39.0 176.0 -86.7 139.9 24.7 23.9 85.7 79 97 A R E + K 0 210C 8 -9,-2.3 2,-0.3 -2,-0.3 -43,-0.2 -0.997 29.5 171.3-149.3 143.0 26.6 23.5 82.4 80 98 A T E + K 0 209C 0 129,-1.7 129,-2.0 -2,-0.3 2,-0.3 -0.888 32.4 160.4-145.7 106.8 29.0 21.2 80.6 81 99 A E E -IK 34 208C 1 -47,-2.4 -47,-0.6 -2,-0.3 -48,-0.5 -0.987 29.6-158.6-139.2 147.1 29.5 22.4 77.0 82 100 A L E -IK 32 207C 0 125,-2.6 125,-3.0 -2,-0.3 -50,-0.2 -0.922 13.9-155.1-118.2 142.9 32.0 21.9 74.2 83 101 A I E -I 31 0C 2 -52,-1.7 -52,-3.7 -2,-0.4 123,-0.1 -0.967 29.0-101.7-121.9 137.0 32.2 24.5 71.3 84 102 A P E -I 30 0C 2 0, 0.0 2,-0.7 0, 0.0 -54,-0.3 -0.317 26.4-149.5 -57.0 135.2 33.5 23.6 67.8 85 103 A Q + 0 0 32 -56,-2.5 2,-0.3 118,-0.0 118,-0.1 -0.955 46.8 128.7-107.3 107.3 37.1 24.8 67.3 86 104 A T - 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