==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=11-MAR-2010 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER MEMBRANE PROTEIN 29-JAN-10 3LMA . COMPND 2 MOLECULE: STAGE V SPORULATION PROTEIN AD (SPOVAD); . SOURCE 2 ORGANISM_SCIENTIFIC: BACILLUS LICHENIFORMIS; . AUTHOR S.VOROBIEV,S.ASHOK,J.SEETHARAMAN,R.L.BELOTE,C.CICCOSANTI, . 1258 11 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 40637.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 912 72.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 93 7.4 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 200 15.9 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 4 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 86 6.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 126 10.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 329 26.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 34 2.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 8 3 5 2 1 1 0 0 0 8 0 0 0 1 4 3 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 4 8 3 1 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 6 8 4 0 0 4 0 8 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 A X 0 0 131 0, 0.0 1082,-0.2 0, 0.0 1081,-0.1 0.000 360.0 360.0 360.0 139.0 3.2 61.2 26.5 2 2 A K + 0 0 23 1080,-2.8 8,-2.7 1079,-0.1 2,-0.4 0.616 360.0 97.1 -92.0 -17.5 1.0 58.1 26.7 3 3 A L E -A 9 0A 28 1079,-1.6 2,-0.5 6,-0.2 6,-0.2 -0.614 61.6-166.0 -70.0 125.1 0.6 58.6 30.4 4 4 A T E > -A 8 0A 34 4,-3.6 4,-1.4 -2,-0.4 3,-0.4 -0.971 54.9 -26.1-117.4 128.8 -2.7 60.4 30.9 5 5 A G T 4 S- 0 0 50 -2,-0.5 196,-0.1 1,-0.2 199,-0.0 -0.386 113.5 -42.0 66.0-145.1 -3.5 61.9 34.2 6 6 A K T 4 S+ 0 0 123 194,-0.2 -1,-0.2 1,-0.1 195,-0.1 0.826 130.5 5.8 -90.7 -30.9 -1.7 60.3 37.1 7 7 A Q T 4 S+ 0 0 7 -3,-0.4 165,-2.3 193,-0.2 2,-0.5 0.308 95.1 105.2-140.6 10.2 -1.9 56.5 36.6 8 8 A T E < -AB 4 171A 0 -4,-1.4 -4,-3.6 163,-0.2 2,-0.6 -0.859 47.7-155.4-110.9 125.4 -3.4 55.7 33.2 9 9 A W E -AB 3 170A 6 161,-3.2 161,-1.8 -2,-0.5 2,-0.7 -0.849 7.9-162.1 -93.2 121.9 -1.6 54.5 30.1 10 10 A E E - B 0 169A 77 -8,-2.7 159,-0.2 -2,-0.6 2,-0.2 -0.911 17.4-137.4-104.7 112.5 -3.4 55.3 26.8 11 11 A F - 0 0 21 157,-1.2 4,-0.1 -2,-0.7 111,-0.1 -0.470 15.6-168.1 -75.2 132.8 -2.1 53.1 24.0 12 12 A E + 0 0 134 -2,-0.2 -1,-0.1 2,-0.1 3,-0.1 0.696 64.1 60.5 -95.1 -20.4 -1.5 55.0 20.8 13 13 A N S S- 0 0 71 1,-0.1 112,-0.1 112,-0.0 113,-0.0 -0.693 103.1 -74.6-107.2 159.5 -1.0 52.2 18.3 14 14 A P + 0 0 78 0, 0.0 2,-0.3 0, 0.0 -1,-0.1 -0.275 56.1 177.5 -52.5 134.5 -3.3 49.3 17.4 15 15 A L + 0 0 4 152,-0.1 152,-2.1 -4,-0.1 2,-0.3 -0.969 1.3 172.7-148.1 123.9 -3.3 46.7 20.2 16 16 A F E -CD 159 166A 25 143,-3.0 143,-1.8 -2,-0.3 2,-0.7 -0.919 39.5-122.6-137.2 156.5 -5.4 43.6 20.4 17 17 A V E -CD 158 165A 6 148,-2.6 148,-0.8 -2,-0.3 147,-0.3 -0.884 31.1-176.6 -89.0 116.7 -6.1 40.4 22.3 18 18 A N E - 0 0 17 139,-2.7 2,-0.3 -2,-0.7 146,-0.2 0.830 61.7 -19.3 -87.8 -33.0 -5.4 37.8 19.5 19 19 A S E -C 157 0A 10 138,-1.1 138,-2.5 -3,-0.1 -1,-0.4 -0.977 57.0-156.8-164.8 163.3 -6.4 34.8 21.5 20 20 A S E -C 156 0A 15 -2,-0.3 2,-0.3 136,-0.2 136,-0.2 -0.948 5.4-157.7-142.6 161.8 -6.9 33.6 25.1 21 21 A G E -C 155 0A 0 134,-2.5 134,-2.6 -2,-0.3 2,-0.3 -0.997 6.0-172.4-143.1 144.5 -6.8 30.3 26.8 22 22 A T E -C 154 0A 0 -2,-0.3 17,-1.8 132,-0.2 16,-0.8 -0.998 5.8-177.4-139.2 144.5 -8.3 29.0 30.1 23 23 A A E +Ce 153 39A 0 130,-2.1 130,-2.1 -2,-0.3 2,-0.3 -0.996 16.7 174.3-138.8 132.0 -7.9 25.8 32.1 24 24 A V E -Ce 152 40A 0 15,-2.0 17,-2.4 -2,-0.4 128,-0.3 -0.872 25.0-113.1-138.5 168.4 -9.8 25.1 35.3 25 25 A G > - 0 0 0 126,-2.2 4,-2.4 -2,-0.3 5,-0.1 -0.161 47.7 -82.1 -86.5-173.4 -10.6 22.5 38.0 26 26 A P H > S+ 0 0 18 0, 0.0 4,-2.2 0, 0.0 5,-0.2 0.845 123.0 56.4 -63.2 -39.8 -13.9 20.7 39.0 27 27 A K H > S+ 0 0 119 1,-0.2 4,-0.6 2,-0.2 7,-0.1 0.925 114.4 41.7 -61.4 -39.8 -15.5 23.4 41.1 28 28 A E H >4 S+ 0 0 2 123,-0.2 3,-0.8 1,-0.2 6,-0.3 0.904 110.8 56.8 -71.6 -40.3 -15.2 25.7 38.1 29 29 A K H 3< S+ 0 0 77 -4,-2.4 -2,-0.2 12,-0.3 -1,-0.2 0.855 108.4 47.6 -57.6 -37.8 -16.3 23.1 35.6 30 30 A E H 3< S+ 0 0 165 -4,-2.2 -1,-0.3 -5,-0.1 -2,-0.2 0.631 98.6 93.4 -79.7 -17.1 -19.5 22.6 37.6 31 31 A G S X< S- 0 0 12 -3,-0.8 3,-1.9 -4,-0.6 4,-0.3 -0.136 98.1 -89.3 -73.0 173.5 -20.2 26.3 37.8 32 32 A P T 3 S+ 0 0 73 0, 0.0 3,-0.2 0, 0.0 218,-0.1 0.720 128.0 43.9 -52.6 -29.9 -22.3 28.5 35.4 33 33 A L T >> S+ 0 0 11 217,-0.5 3,-2.7 -5,-0.2 4,-0.6 0.400 78.6 114.5-100.5 3.7 -19.3 29.2 33.2 34 34 A G G X4 S+ 0 0 4 -3,-1.9 3,-0.7 1,-0.3 -1,-0.2 0.776 74.3 51.2 -43.7 -42.7 -18.0 25.6 33.1 35 35 A H G 34 S+ 0 0 149 -4,-0.3 -1,-0.3 -3,-0.2 -2,-0.1 0.615 100.4 65.3 -74.8 -12.1 -18.6 25.1 29.4 36 36 A L G <4 S+ 0 0 32 -3,-2.7 -1,-0.2 -7,-0.0 -2,-0.2 0.662 79.9 91.7 -86.4 -16.4 -16.7 28.2 28.4 37 37 A F S << S- 0 0 12 -3,-0.7 -14,-0.1 -4,-0.6 3,-0.1 -0.470 74.5-134.4 -79.5 151.2 -13.2 27.2 29.6 38 38 A D S S+ 0 0 59 -16,-0.8 2,-0.4 1,-0.2 -15,-0.2 0.871 93.1 22.3 -75.1 -38.2 -10.9 25.5 27.1 39 39 A K E +e 23 0A 101 -17,-1.8 -15,-2.0 19,-0.1 2,-0.3 -0.983 68.5 173.0-126.7 140.3 -9.8 22.8 29.5 40 40 A S E -e 24 0A 37 -2,-0.4 2,-0.3 -17,-0.2 -15,-0.2 -0.878 17.7-147.9-151.5 118.4 -11.8 21.7 32.6 41 41 A Y - 0 0 42 -17,-2.4 -12,-0.3 -2,-0.3 -13,-0.1 -0.655 13.7-143.7 -95.9 142.0 -10.9 18.7 34.8 42 42 A D S S+ 0 0 137 -2,-0.3 2,-0.3 -17,-0.1 -1,-0.1 0.607 88.9 36.3 -72.2 -14.3 -13.2 16.5 36.7 43 43 A E S > S- 0 0 98 -17,-0.0 3,-2.2 1,-0.0 4,-0.2 -0.995 85.0-115.2-145.3 148.6 -10.8 16.1 39.6 44 44 A X T 3 S+ 0 0 53 -2,-0.3 97,-0.1 1,-0.3 -3,-0.0 0.595 107.0 65.3 -66.5 -14.8 -8.4 18.5 41.3 45 45 A H T > S- 0 0 59 9,-0.1 3,-1.6 4,-0.0 -1,-0.3 0.641 94.4-140.1 -77.9 -13.0 -5.1 16.8 40.4 46 46 A C T < - 0 0 0 -3,-2.2 -2,-0.1 1,-0.3 3,-0.1 0.884 67.5 -58.7 53.3 40.6 -5.9 17.6 36.8 47 47 A N T 3 S+ 0 0 148 1,-0.2 -1,-0.3 -4,-0.2 2,-0.2 0.684 112.8 127.1 63.6 23.6 -4.6 14.1 35.9 48 48 A Q S < S- 0 0 53 -3,-1.6 -1,-0.2 2,-0.1 6,-0.1 -0.639 72.8-113.3-111.0 165.6 -1.3 15.1 37.5 49 49 A K S S- 0 0 187 -2,-0.2 2,-0.2 -3,-0.1 -1,-0.1 0.842 89.7 -15.2 -64.4 -37.3 1.0 13.5 40.1 50 50 A N S > S- 0 0 48 -5,-0.2 4,-1.9 -4,-0.1 5,-0.1 -0.873 79.6 -79.0-155.6-175.1 0.6 16.3 42.7 51 51 A W H > S+ 0 0 78 -2,-0.2 4,-2.3 2,-0.2 5,-0.2 0.855 122.6 51.7 -67.2 -35.9 -0.6 19.9 43.3 52 52 A E H > S+ 0 0 38 2,-0.2 4,-2.2 1,-0.2 -1,-0.2 0.966 114.0 41.9 -65.5 -53.5 2.5 21.5 41.9 53 53 A X H > S+ 0 0 54 1,-0.2 4,-2.4 2,-0.2 -1,-0.2 0.815 111.4 60.0 -64.8 -27.0 2.3 19.6 38.6 54 54 A A H X S+ 0 0 0 -4,-1.9 4,-2.1 2,-0.2 -2,-0.2 0.957 108.8 40.5 -63.7 -53.1 -1.5 20.1 38.6 55 55 A E H X S+ 0 0 3 -4,-2.3 4,-2.1 2,-0.2 -2,-0.2 0.843 114.1 54.3 -64.9 -34.7 -1.2 23.9 38.5 56 56 A R H X S+ 0 0 5 -4,-2.2 4,-2.6 2,-0.2 -1,-0.2 0.913 108.3 49.6 -64.7 -41.5 1.8 23.7 36.1 57 57 A K H X S+ 0 0 63 -4,-2.4 4,-2.9 2,-0.2 -2,-0.2 0.905 107.9 54.8 -62.2 -42.0 -0.5 21.6 33.8 58 58 A L H X S+ 0 0 0 -4,-2.1 4,-2.4 2,-0.2 -2,-0.2 0.931 112.2 42.2 -59.1 -45.6 -3.3 24.2 34.1 59 59 A X H X S+ 0 0 0 -4,-2.1 4,-2.9 2,-0.2 5,-0.2 0.920 114.0 50.8 -68.5 -44.5 -0.9 27.0 32.9 60 60 A E H X S+ 0 0 65 -4,-2.6 4,-2.7 1,-0.2 5,-0.2 0.918 112.3 49.3 -56.8 -43.8 0.6 24.9 30.2 61 61 A D H X S+ 0 0 12 -4,-2.9 4,-2.3 2,-0.2 -2,-0.2 0.948 112.3 46.6 -58.2 -50.2 -2.9 24.1 29.0 62 62 A A H X S+ 0 0 0 -4,-2.4 4,-2.2 -5,-0.2 93,-0.2 0.920 114.4 47.3 -62.8 -43.4 -4.0 27.7 29.0 63 63 A V H X S+ 0 0 3 -4,-2.9 4,-2.7 1,-0.2 -1,-0.2 0.929 112.7 48.0 -62.4 -46.6 -0.9 28.9 27.2 64 64 A Q H X S+ 0 0 99 -4,-2.7 4,-2.8 -5,-0.2 -1,-0.2 0.848 109.8 53.7 -68.1 -30.0 -1.2 26.1 24.5 65 65 A S H X S+ 0 0 21 -4,-2.3 4,-1.9 -5,-0.2 -1,-0.2 0.960 110.8 46.1 -64.8 -49.8 -4.9 26.9 24.0 66 66 A A H < S+ 0 0 0 -4,-2.2 4,-0.5 1,-0.2 -2,-0.2 0.945 116.6 44.2 -58.0 -48.4 -4.1 30.5 23.4 67 67 A L H ><>S+ 0 0 2 -4,-2.7 5,-2.5 1,-0.2 3,-1.5 0.917 110.5 55.6 -62.3 -42.7 -1.2 29.7 21.0 68 68 A S H ><5S+ 0 0 70 -4,-2.8 3,-1.3 1,-0.3 -1,-0.2 0.844 97.2 63.0 -58.4 -38.4 -3.2 27.0 19.2 69 69 A K T 3<5S+ 0 0 97 -4,-1.9 -1,-0.3 1,-0.3 -2,-0.2 0.697 115.3 32.2 -64.5 -18.1 -6.0 29.3 18.3 70 70 A Q T < 5S- 0 0 60 -3,-1.5 -1,-0.3 -4,-0.5 -2,-0.2 0.273 110.6-117.3-117.9 11.7 -3.5 31.4 16.3 71 71 A N T < 5 + 0 0 109 -3,-1.3 2,-0.3 -4,-0.3 -3,-0.2 0.922 62.5 156.4 48.7 49.1 -1.3 28.4 15.2 72 72 A L < - 0 0 8 -5,-2.5 2,-0.3 -8,-0.1 -1,-0.2 -0.780 35.6-147.6-104.1 147.0 1.6 29.9 17.0 73 73 A K > - 0 0 106 -2,-0.3 3,-1.8 1,-0.1 4,-0.2 -0.699 34.9-105.5-100.4 163.1 4.7 28.2 18.4 74 74 A K G > S+ 0 0 62 1,-0.3 3,-1.8 -2,-0.3 -1,-0.1 0.853 120.2 63.7 -55.3 -35.9 6.5 29.4 21.6 75 75 A E G 3 S+ 0 0 81 1,-0.3 -1,-0.3 26,-0.1 25,-0.0 0.697 90.6 67.8 -64.2 -16.3 9.3 30.9 19.3 76 76 A D G < S+ 0 0 3 -3,-1.8 2,-0.4 706,-0.1 -1,-0.3 0.486 86.3 83.2 -82.1 -3.9 6.7 33.3 17.8 77 77 A I < - 0 0 5 -3,-1.8 55,-0.2 -4,-0.2 3,-0.1 -0.863 58.1-166.3-104.9 135.3 6.4 35.1 21.1 78 78 A D S S+ 0 0 12 53,-1.9 2,-0.3 -2,-0.4 54,-0.2 0.807 71.6 19.2 -87.7 -33.1 8.8 37.9 22.0 79 79 A I E -f 132 0A 2 52,-1.2 54,-2.4 22,-0.1 2,-0.5 -0.998 57.7-156.8-146.3 143.6 8.0 38.4 25.7 80 80 A F E -fg 133 103A 0 22,-2.3 24,-2.8 -2,-0.3 2,-0.5 -0.988 9.1-173.6-124.9 121.5 6.3 36.4 28.5 81 81 A L E +fg 134 104A 0 52,-2.7 54,-1.9 -2,-0.5 2,-0.3 -0.974 26.8 135.2-117.6 120.3 4.9 38.1 31.6 82 82 A A E +fg 135 105A 0 22,-1.3 24,-2.7 -2,-0.5 2,-0.3 -0.988 21.4 167.4-160.2 161.0 3.6 35.9 34.3 83 83 A G E -f 136 0A 0 52,-1.2 54,-0.7 -2,-0.3 2,-0.3 -0.950 43.1 -91.6-164.9 163.4 3.3 35.1 38.0 84 84 A D E -f 137 0A 0 22,-0.3 54,-0.2 3,-0.3 28,-0.1 -0.623 25.9-140.7 -78.9 145.7 1.3 32.9 40.3 85 85 A L S S+ 0 0 9 52,-3.2 53,-0.2 -2,-0.3 26,-0.1 0.795 97.3 11.8 -75.6 -25.4 -1.8 34.6 41.7 86 86 A L S S+ 0 0 15 51,-0.6 52,-0.1 23,-0.1 24,-0.1 0.798 118.3 50.8-116.4 -49.4 -1.1 32.9 45.1 87 87 A N S S- 0 0 28 1,-0.1 2,-0.7 22,-0.1 21,-0.3 -0.019 94.5 -78.3 -93.1-169.5 2.3 31.3 45.5 88 88 A Q S S- 0 0 40 1,-0.1 -1,-0.1 19,-0.1 -3,-0.0 -0.020 79.9 -82.6 -85.8 42.5 6.0 32.3 44.9 89 89 A N S > S+ 0 0 0 -2,-0.7 4,-2.3 3,-0.0 5,-0.3 0.637 82.7 147.3 63.7 27.8 5.9 31.9 41.1 90 90 A V T 4 S+ 0 0 11 1,-0.2 4,-0.3 2,-0.2 1156,-0.1 0.749 77.5 33.7 -57.2 -30.5 6.5 28.0 41.5 91 91 A T T > S+ 0 0 2 2,-0.1 4,-2.5 3,-0.1 3,-0.4 0.890 114.1 55.4 -91.5 -51.9 4.4 27.4 38.4 92 92 A A H > S+ 0 0 0 1,-0.2 4,-2.6 2,-0.2 -2,-0.2 0.854 107.3 49.2 -52.9 -44.3 5.3 30.4 36.3 93 93 A N H X S+ 0 0 7 -4,-2.3 4,-2.0 2,-0.2 -1,-0.2 0.870 111.2 50.1 -65.7 -38.7 9.1 29.9 36.4 94 94 A Y H > S+ 0 0 42 -3,-0.4 4,-1.7 -4,-0.3 -2,-0.2 0.931 113.6 45.0 -64.3 -48.1 8.7 26.2 35.4 95 95 A V H X S+ 0 0 2 -4,-2.5 4,-2.3 1,-0.2 5,-0.2 0.905 111.4 53.6 -64.4 -39.3 6.4 27.1 32.4 96 96 A A H X S+ 0 0 0 -4,-2.6 4,-2.2 -5,-0.3 5,-0.4 0.895 105.0 55.3 -62.0 -38.3 8.8 29.9 31.4 97 97 A R H < S+ 0 0 68 -4,-2.0 -1,-0.2 1,-0.2 -2,-0.2 0.925 110.8 44.8 -61.5 -42.7 11.7 27.5 31.4 98 98 A H H < S+ 0 0 65 -4,-1.7 -1,-0.2 1,-0.2 -2,-0.2 0.872 113.7 47.7 -70.4 -35.2 9.8 25.2 29.0 99 99 A L H < S- 0 0 22 -4,-2.3 -1,-0.2 -5,-0.1 -2,-0.2 0.792 88.9-151.8 -75.3 -28.4 8.6 28.0 26.6 100 100 A K < + 0 0 113 -4,-2.2 -3,-0.1 1,-0.2 -4,-0.1 0.648 42.8 145.3 64.7 15.9 12.0 29.6 26.4 101 101 A I - 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