==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=24-JUL-2010 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER IMMUNE SYSTEM 12-FEB-10 3LS5 . COMPND 2 MOLECULE: LIGHT CHAIN OF ANTIBODY FAB FRAGMENT; . SOURCE 2 ORGANISM_SCIENTIFIC: MUS MUSCULUS; . AUTHOR M.H.NIEMI,J.ROUVINEN . 434 2 5 4 1 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 19618.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 279 64.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 8 1.8 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 175 40.3 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 3 0.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 42 9.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 34 7.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 13 3.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 4 3 7 2 4 9 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 1 0 5 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 L D 0 0 145 0, 0.0 2,-0.3 0, 0.0 96,-0.1 0.000 360.0 360.0 360.0 129.1 15.5 14.3 -30.9 2 2 L I - 0 0 15 94,-0.4 2,-0.5 89,-0.1 89,-0.1 -0.558 360.0-153.4 -67.1 130.5 17.0 11.7 -33.2 3 3 L V - 0 0 85 -2,-0.3 23,-2.3 95,-0.1 2,-0.6 -0.945 3.3-154.8-106.1 129.1 17.3 8.3 -31.4 4 4 L L E -A 25 0A 9 -2,-0.5 97,-0.6 95,-0.4 2,-0.6 -0.946 6.4-162.5-108.2 120.0 20.1 6.1 -32.7 5 5 L T E -A 24 0A 89 19,-2.8 19,-2.5 -2,-0.6 2,-0.3 -0.915 8.0-160.1-107.6 117.4 19.5 2.4 -32.1 6 6 L Q E -A 23 0A 14 -2,-0.6 17,-0.2 17,-0.2 95,-0.0 -0.726 12.7-122.0 -98.8 143.5 22.5 0.1 -32.4 7 7 L S E S+A 22 0A 60 15,-2.1 15,-3.2 -2,-0.3 2,-0.2 -0.993 77.6 20.6-144.8 153.8 22.6 -3.6 -32.9 8 8 L P - 0 0 45 0, 0.0 -1,-0.1 0, 0.0 3,-0.1 0.735 67.3-148.7 -76.7 176.3 23.6 -5.9 -31.9 9 9 L T S S+ 0 0 110 1,-0.2 95,-2.2 -2,-0.2 2,-0.3 0.846 85.7 8.5 -70.5 -34.6 24.0 -4.7 -28.3 10 10 L T E S-d 104 0B 63 93,-0.2 2,-0.3 76,-0.0 -1,-0.2 -0.987 70.3-172.7-144.7 147.6 26.9 -7.1 -27.8 11 11 L M E -d 105 0B 42 93,-2.3 95,-3.1 -2,-0.3 2,-0.5 -0.985 14.3-152.9-146.4 139.1 28.8 -9.3 -30.3 12 12 L A E -d 106 0B 44 -2,-0.3 2,-0.4 93,-0.2 95,-0.2 -0.958 22.0-168.7-110.4 122.9 31.4 -11.9 -29.9 13 13 L A E -d 107 0B 0 93,-2.7 95,-2.5 -2,-0.5 3,-0.1 -0.928 19.2-127.8-116.5 135.3 33.7 -12.3 -32.9 14 14 L S > - 0 0 48 -2,-0.4 3,-2.2 93,-0.2 65,-0.3 -0.510 46.0 -88.3 -69.6 148.1 36.2 -14.9 -33.8 15 15 L P T 3 S+ 0 0 85 0, 0.0 65,-0.2 0, 0.0 -1,-0.1 -0.294 117.7 19.5 -51.7 137.0 39.7 -13.7 -34.7 16 16 L G T 3 S+ 0 0 46 63,-3.1 2,-0.2 1,-0.2 64,-0.1 0.306 93.2 140.3 80.5 -8.1 39.7 -13.0 -38.5 17 17 L E < - 0 0 77 -3,-2.2 62,-2.3 61,-0.2 2,-0.6 -0.445 57.1-123.9 -72.4 139.9 35.9 -12.8 -38.7 18 18 L K - 0 0 150 60,-0.2 2,-0.4 -2,-0.2 59,-0.2 -0.752 37.4-166.9 -84.8 120.6 34.3 -10.2 -41.0 19 19 L I E - B 0 76A 6 57,-2.4 57,-2.8 -2,-0.6 2,-0.4 -0.903 14.6-174.2-118.5 137.4 32.0 -8.2 -38.6 20 20 L T E - B 0 75A 65 -2,-0.4 2,-0.3 55,-0.2 55,-0.2 -0.999 3.2-176.8-134.0 129.7 29.2 -5.7 -39.4 21 21 L I E - B 0 74A 5 53,-2.7 53,-2.3 -2,-0.4 2,-0.3 -0.915 14.3-140.7-126.0 156.4 27.3 -3.7 -36.8 22 22 L T E -AB 7 73A 56 -15,-3.2 -15,-2.1 -2,-0.3 2,-0.4 -0.803 14.3-163.0-129.6 159.5 24.4 -1.3 -37.4 23 23 L a E -AB 6 72A 1 49,-2.3 49,-2.4 -2,-0.3 2,-0.5 -0.976 12.9-164.0-140.3 118.6 22.9 2.0 -36.4 24 24 L S E -AB 5 71A 57 -19,-2.5 -19,-2.8 -2,-0.4 2,-0.5 -0.922 9.1-147.6-113.1 124.6 19.3 2.9 -37.2 25 25 L A E -A 4 0A 4 45,-2.4 -21,-0.2 -2,-0.5 4,-0.1 -0.809 15.1-138.0 -95.9 129.5 18.1 6.5 -36.9 26 26 L S E S+ 0 0 83 -23,-2.3 2,-0.3 -2,-0.5 -1,-0.1 0.602 94.5 23.4 -53.7 -18.1 14.5 7.3 -35.9 27 27 L S E S- 0 0 65 -24,-0.2 2,-0.2 42,-0.1 -1,-0.1 -0.985 104.3 -79.1-149.5 154.1 14.6 10.0 -38.7 28 28 L S E - 0 0 82 -2,-0.3 2,-0.4 1,-0.1 42,-0.2 -0.300 44.8-160.3 -64.7 122.9 16.6 10.4 -41.9 29 29 L I E - B 0 69A 3 40,-2.3 2,-0.7 -2,-0.2 40,-0.5 -0.778 26.2-113.6 -96.1 134.3 20.1 11.8 -41.4 30 30 L S > - 0 0 63 -2,-0.4 3,-3.4 1,-0.2 62,-0.2 -0.626 29.7-130.1 -63.6 118.2 22.2 13.5 -44.0 31 31 L S G > S+ 0 0 44 -2,-0.7 3,-1.3 1,-0.3 -1,-0.2 0.657 103.6 74.4 -39.5 -30.2 25.0 10.9 -44.4 32 32 L N G 3 S+ 0 0 69 1,-0.2 -1,-0.3 19,-0.1 19,-0.2 0.645 98.9 45.8 -55.0 -20.2 27.4 13.9 -44.0 33 33 L Y G < S+ 0 0 77 -3,-3.4 59,-2.5 18,-0.1 2,-0.5 0.024 86.4 100.5-119.1 23.3 26.5 14.0 -40.3 34 34 L L E < +E 91 0B 0 -3,-1.3 18,-3.4 57,-0.2 2,-0.3 -0.965 47.3 166.8-115.6 119.5 26.8 10.4 -39.3 35 35 L H E -E 90 0B 0 55,-2.5 55,-2.0 -2,-0.5 2,-0.4 -0.832 24.3-140.8-130.7 162.5 30.0 9.4 -37.5 36 36 L W E -EF 89 49B 0 13,-2.6 12,-3.1 -2,-0.3 13,-1.8 -0.974 10.9-165.1-126.3 145.0 31.4 6.5 -35.5 37 37 L Y E -EF 88 47B 2 51,-2.5 51,-2.2 -2,-0.4 2,-0.5 -0.914 9.5-146.8-125.2 153.8 33.7 6.4 -32.5 38 38 L Q E -EF 87 46B 8 8,-2.8 8,-1.5 -2,-0.3 2,-0.6 -0.975 9.8-167.8-121.4 129.6 35.6 3.6 -30.9 39 39 L Q E -E 86 0B 20 47,-3.2 47,-2.3 -2,-0.5 3,-0.1 -0.914 6.4-158.2-121.6 108.1 36.2 3.4 -27.2 40 40 L K > - 0 0 67 -2,-0.6 3,-2.3 4,-0.5 45,-0.2 -0.538 42.0 -85.2 -79.7 147.5 38.7 1.0 -26.0 41 41 L P T 3 S+ 0 0 63 0, 0.0 -1,-0.1 0, 0.0 3,-0.1 -0.298 116.1 17.8 -60.7 135.1 38.3 -0.1 -22.3 42 42 L G T 3 S+ 0 0 80 1,-0.3 2,-0.2 -3,-0.1 -2,-0.0 0.359 115.3 87.0 87.9 -4.5 39.9 2.4 -19.9 43 43 L F S < S- 0 0 142 -3,-2.3 -1,-0.3 1,-0.1 3,-0.1 -0.719 80.3 -92.4-128.5 173.5 40.1 5.1 -22.6 44 44 L S - 0 0 28 -2,-0.2 -4,-0.5 1,-0.1 -6,-0.1 -0.480 52.4 -94.6 -77.4 154.2 38.3 8.0 -24.1 45 45 L P - 0 0 3 0, 0.0 2,-0.3 0, 0.0 -6,-0.2 -0.334 42.6-154.1 -61.1 159.0 36.2 7.5 -27.2 46 46 L K E -F 38 0B 76 -8,-1.5 -8,-2.8 -3,-0.1 2,-0.1 -0.987 23.9-103.6-136.4 138.8 38.2 8.2 -30.5 47 47 L L E +F 37 0B 14 -2,-0.3 -10,-0.2 273,-0.2 3,-0.1 -0.451 45.0 165.8 -63.5 131.4 36.7 9.3 -33.9 48 48 L L E + 0 0 0 -12,-3.1 8,-3.0 1,-0.5 2,-0.4 0.710 63.0 13.0-116.6 -49.4 36.9 6.2 -36.1 49 49 L I E -FG 36 55B 0 -13,-1.8 -13,-2.6 6,-0.2 -1,-0.5 -0.994 65.4-167.8-132.5 137.4 34.7 7.0 -39.1 50 50 L Y E >> + G 0 54B 75 4,-2.9 4,-2.0 -2,-0.4 3,-0.5 -0.961 68.4 14.5-127.2 152.8 33.3 10.4 -40.2 51 51 L R T 34 S- 0 0 96 -2,-0.3 2,-2.1 1,-0.2 -16,-0.2 0.935 123.3 -69.6 52.3 52.3 30.7 11.3 -42.7 52 52 L T T 34 S+ 0 0 9 -18,-3.4 -1,-0.2 1,-0.2 21,-0.2 -0.235 129.2 23.8 72.7 -46.9 29.4 7.7 -43.0 53 53 L S T <4 S+ 0 0 67 -2,-2.1 2,-0.5 -3,-0.5 12,-0.3 0.348 86.7 110.1-138.3 -0.7 32.4 6.2 -44.8 54 54 L N E < -G 50 0B 43 -4,-2.0 -4,-2.9 10,-0.1 2,-0.2 -0.747 60.8-130.7 -94.2 130.2 35.6 8.2 -44.2 55 55 L L E -G 49 0B 61 -2,-0.5 -6,-0.2 -6,-0.2 3,-0.1 -0.492 22.7-132.9 -76.7 133.4 38.3 6.7 -42.0 56 56 L A > - 0 0 12 -8,-3.0 3,-1.7 -2,-0.2 -1,-0.1 -0.207 44.0 -62.1 -75.7 175.3 39.6 9.0 -39.3 57 57 L S T 3 S+ 0 0 118 1,-0.2 -1,-0.2 264,-0.1 -10,-0.0 -0.298 121.5 9.5 -60.6 136.7 43.3 9.6 -38.6 58 58 L G T 3 S+ 0 0 72 1,-0.2 -1,-0.2 -3,-0.1 -2,-0.1 0.463 90.6 137.9 75.6 4.5 45.2 6.5 -37.5 59 59 L V < - 0 0 16 -3,-1.7 -1,-0.2 -11,-0.1 -4,-0.1 -0.695 55.2-117.0 -90.1 125.6 42.3 4.1 -38.3 60 60 L P > - 0 0 36 0, 0.0 3,-2.0 0, 0.0 -1,-0.1 -0.197 20.8-116.1 -59.6 149.5 43.3 0.8 -40.0 61 61 L A T 3 S+ 0 0 96 1,-0.3 -2,-0.0 15,-0.0 -6,-0.0 0.687 105.7 77.5 -57.4 -23.9 42.1 -0.0 -43.6 62 62 L R T 3 S+ 0 0 54 14,-0.1 15,-2.9 16,-0.1 2,-0.3 0.645 81.7 86.9 -67.8 -10.6 40.1 -3.1 -42.4 63 63 L F E < +C 76 0A 5 -3,-2.0 2,-0.3 13,-0.2 13,-0.2 -0.678 53.7 159.7 -84.2 143.3 37.5 -0.6 -41.1 64 64 L S E -C 75 0A 58 11,-2.0 11,-2.8 -2,-0.3 2,-0.3 -0.983 19.6-158.8-153.9 161.9 34.7 0.7 -43.4 65 65 L G E +C 74 0A 10 -2,-0.3 2,-0.3 -12,-0.3 9,-0.2 -0.979 14.1 166.9-139.9 155.0 31.3 2.3 -43.0 66 66 L S E +C 73 0A 62 7,-2.2 7,-2.8 -2,-0.3 2,-0.2 -0.951 18.4 95.3-156.1 174.0 28.3 2.7 -45.3 67 67 L G E -C 72 0A 34 -2,-0.3 2,-0.3 5,-0.2 5,-0.2 -0.690 32.9-150.8 130.5 176.6 24.7 3.6 -45.3 68 68 L S E > +C 71 0A 83 3,-1.9 3,-2.1 -2,-0.2 2,-0.5 -0.937 61.9 18.7-174.0 165.1 22.3 6.5 -46.0 69 69 L G E 3 S-B 29 0A 25 -40,-0.5 -40,-2.3 -2,-0.3 -42,-0.1 -0.485 129.9 -22.9 70.8-119.6 18.9 7.7 -44.8 70 70 L T E 3 S+ 0 0 56 -2,-0.5 -45,-2.4 -42,-0.2 2,-0.5 0.350 123.8 79.1-105.4 2.1 18.0 6.0 -41.5 71 71 L S E < +BC 24 68A 75 -3,-2.1 -3,-1.9 -47,-0.2 2,-0.3 -0.932 50.7 162.3-124.8 118.0 20.4 3.0 -41.9 72 72 L Y E -BC 23 67A 3 -49,-2.4 -49,-2.3 -2,-0.5 2,-0.3 -0.887 11.1-170.2-128.2 155.4 24.1 3.0 -41.3 73 73 L S E -BC 22 66A 20 -7,-2.8 -7,-2.2 -2,-0.3 2,-0.4 -0.987 14.8-149.9-148.0 162.9 26.7 0.3 -40.7 74 74 L L E -BC 21 65A 3 -53,-2.3 -53,-2.7 -2,-0.3 2,-0.4 -0.985 17.9-165.1-132.6 133.0 30.3 -0.3 -39.7 75 75 L T E -BC 20 64A 20 -11,-2.8 -11,-2.0 -2,-0.4 2,-0.6 -0.974 14.2-160.9-127.4 135.9 32.4 -3.2 -40.9 76 76 L I E -BC 19 63A 4 -57,-2.8 -57,-2.4 -2,-0.4 3,-0.3 -0.979 15.6-149.4-112.8 123.8 35.6 -4.6 -39.7 77 77 L G S S+ 0 0 31 -15,-2.9 2,-0.3 -2,-0.6 -1,-0.1 0.866 83.8 4.5 -64.9 -38.9 37.1 -6.7 -42.5 78 78 L T S S- 0 0 57 -60,-0.1 -1,-0.3 -3,-0.1 -60,-0.2 -0.931 84.7-118.6-147.3 115.5 38.8 -9.2 -40.2 79 79 L M - 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