==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=20-JUL-2011 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 12-FEB-10 3LSA . COMPND 2 MOLECULE: PADRON0.9; . SOURCE 2 ORGANISM_SCIENTIFIC: PECTINIIDAE; . AUTHOR T.BRAKEMANN,G.WEBER,S.TROWITZSCH,M.C.WAHL,S.JAKOBS . 863 8 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 33996.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 651 75.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 52 6.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 408 47.3 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 12 1.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 77 8.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 65 7.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 31 3.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 4 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 0 4 0 8 0 4 4 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 2 A S 0 0 150 0, 0.0 3,-0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 160.6 -4.6 43.8 -27.6 2 3 A V + 0 0 54 75,-0.2 2,-0.8 78,-0.1 75,-0.0 0.525 360.0 91.9-105.7 -11.0 -7.0 42.0 -25.2 3 4 A I - 0 0 21 77,-0.1 106,-0.1 74,-0.1 4,-0.1 -0.784 61.1-168.1 -89.9 108.2 -5.9 38.4 -25.9 4 5 A K - 0 0 104 -2,-0.8 29,-0.2 1,-0.1 3,-0.1 -0.560 33.1-115.2 -91.5 158.2 -3.1 37.5 -23.4 5 6 A P S S+ 0 0 91 0, 0.0 2,-0.4 0, 0.0 28,-0.3 0.802 108.0 40.1 -65.9 -26.6 -0.8 34.4 -23.7 6 7 A D S S+ 0 0 98 25,-0.2 2,-0.3 26,-0.1 25,-0.2 -0.989 76.8 173.5-123.8 124.8 -2.3 33.1 -20.4 7 8 A M E -A 30 0A 2 23,-2.5 23,-3.1 -2,-0.4 2,-0.2 -0.920 20.8-134.7-131.4 152.9 -6.0 33.5 -19.8 8 9 A K E -Ab 29 110A 108 101,-2.8 103,-2.6 -2,-0.3 2,-0.4 -0.593 13.6-145.5-105.6 165.9 -8.5 32.3 -17.1 9 10 A I E -Ab 28 111A 5 19,-2.3 19,-2.2 101,-0.2 2,-0.4 -0.997 13.8-178.9-137.1 133.8 -12.0 30.8 -17.4 10 11 A K E +Ab 27 112A 117 101,-2.8 103,-2.5 -2,-0.4 2,-0.3 -0.998 21.2 167.7-128.6 127.2 -15.2 31.1 -15.3 11 12 A L E -Ab 26 113A 12 15,-2.4 15,-2.6 -2,-0.4 2,-0.3 -0.979 24.3-169.5-142.1 154.9 -18.3 29.1 -16.4 12 13 A R E -Ab 25 114A 70 101,-1.8 103,-2.9 -2,-0.3 2,-0.5 -0.979 9.3-165.7-138.9 130.3 -21.7 27.9 -15.4 13 14 A M E -Ab 24 115A 0 11,-3.2 11,-2.4 -2,-0.3 2,-0.4 -0.962 4.0-174.8-111.1 131.3 -23.9 25.4 -17.3 14 15 A E E +Ab 23 116A 88 101,-2.5 103,-1.9 -2,-0.5 2,-0.3 -0.979 28.8 120.2-117.9 139.9 -27.6 25.0 -16.5 15 16 A G E -Ab 22 117A 2 7,-2.3 7,-2.3 -2,-0.4 2,-0.3 -0.932 44.7-121.0-167.0-166.4 -29.5 22.2 -18.3 16 17 A A E -Ab 21 118A 8 101,-2.0 103,-2.4 5,-0.3 2,-0.4 -0.975 7.6-156.9-154.1 150.9 -31.6 19.1 -18.4 17 18 A V E > S-Ab 20 119A 2 3,-1.5 3,-1.7 -2,-0.3 103,-0.2 -0.990 87.2 -11.6-132.8 125.1 -31.2 15.6 -19.9 18 19 A N T 3 S- 0 0 9 101,-2.5 102,-0.2 -2,-0.4 -1,-0.1 0.850 132.7 -54.8 48.9 36.8 -34.4 13.6 -20.5 19 20 A G T 3 S+ 0 0 32 100,-0.6 -1,-0.3 1,-0.2 101,-0.1 0.500 110.5 121.4 82.8 5.3 -36.2 16.3 -18.4 20 21 A H E < -A 17 0A 124 -3,-1.7 -3,-1.5 26,-0.0 -1,-0.2 -0.894 56.4-137.9-108.3 111.8 -34.0 15.9 -15.3 21 22 A P E +A 16 0A 90 0, 0.0 2,-0.3 0, 0.0 -5,-0.3 -0.251 27.5 165.6 -75.0 150.1 -32.3 19.3 -14.5 22 23 A F E -A 15 0A 6 -7,-2.3 -7,-2.3 27,-0.0 2,-0.3 -0.959 20.0-150.0-150.9 166.7 -28.7 19.9 -13.4 23 24 A A E -AC 14 45A 0 22,-2.2 21,-3.0 -2,-0.3 22,-1.8 -0.988 10.4-173.8-146.0 138.6 -26.1 22.6 -12.9 24 25 A I E -AC 13 43A 0 -11,-2.4 -11,-3.2 -2,-0.3 2,-0.3 -0.987 5.7-160.5-133.0 138.8 -22.4 22.6 -13.2 25 26 A E E -AC 12 42A 76 17,-2.7 17,-3.2 -2,-0.4 2,-0.3 -0.916 11.4-170.5-118.7 150.0 -19.8 25.3 -12.5 26 27 A G E -AC 11 41A 7 -15,-2.6 -15,-2.4 -2,-0.3 2,-0.4 -0.980 17.4-169.2-141.0 149.3 -16.2 25.5 -13.7 27 28 A V E +AC 10 40A 91 13,-1.9 13,-3.0 -2,-0.3 2,-0.3 -0.993 28.5 156.9-131.2 140.8 -12.9 27.4 -13.3 28 29 A G E -AC 9 39A 7 -19,-2.2 -19,-2.3 -2,-0.4 2,-0.4 -0.925 34.4-128.5-155.6 178.0 -10.0 27.0 -15.6 29 30 A L E +AC 8 38A 112 9,-2.7 9,-2.7 -2,-0.3 2,-0.3 -0.991 27.9 174.7-137.5 139.9 -6.8 28.2 -17.3 30 31 A G E -AC 7 37A 5 -23,-3.1 -23,-2.5 -2,-0.4 7,-0.3 -0.940 32.2-138.2-145.1 165.5 -5.9 28.3 -21.0 31 32 A K E >>> - C 0 36A 82 5,-3.4 4,-1.7 -2,-0.3 5,-1.4 -0.880 17.7-167.7-125.2 94.8 -3.4 29.4 -23.6 32 33 A P T 345S+ 0 0 0 0, 0.0 34,-0.6 0, 0.0 -1,-0.1 0.822 87.3 45.1 -58.8 -34.1 -5.2 31.0 -26.5 33 34 A F T 345S+ 0 0 113 -28,-0.3 -27,-0.1 1,-0.2 32,-0.1 0.734 115.1 47.4 -81.8 -20.9 -2.2 31.0 -28.8 34 35 A E T <45S- 0 0 102 -3,-0.8 33,-0.3 -29,-0.3 -1,-0.2 0.622 106.4-127.2 -90.7 -15.2 -1.2 27.5 -28.0 35 36 A G T <5 + 0 0 0 -4,-1.7 175,-2.5 31,-0.3 2,-0.4 0.874 65.9 125.2 71.1 39.7 -4.8 26.1 -28.4 36 37 A K E < +CD 31 209A 60 -5,-1.4 -5,-3.4 173,-0.2 2,-0.3 -0.980 29.0 165.2-131.7 142.5 -4.8 24.5 -24.9 37 38 A Q E -CD 30 208A 8 171,-2.0 171,-2.6 -2,-0.4 2,-0.3 -0.988 12.5-167.9-149.5 155.3 -7.1 24.8 -21.9 38 39 A S E +CD 29 207A 47 -9,-2.7 -9,-2.7 -2,-0.3 2,-0.3 -0.994 12.7 179.9-138.6 143.2 -7.8 22.9 -18.7 39 40 A M E -CD 28 206A 6 167,-2.5 167,-2.7 -2,-0.3 2,-0.5 -0.998 25.7-134.7-145.0 152.5 -10.7 23.3 -16.3 40 41 A D E -CD 27 205A 95 -13,-3.0 -13,-1.9 -2,-0.3 2,-0.4 -0.914 27.2-162.0-103.0 126.9 -12.0 21.9 -13.0 41 42 A L E -CD 26 204A 2 163,-3.3 163,-2.4 -2,-0.5 2,-0.4 -0.902 7.0-169.4-116.7 136.8 -15.8 21.2 -13.1 42 43 A K E -CD 25 203A 99 -17,-3.2 -17,-2.7 -2,-0.4 2,-0.4 -0.987 22.1-130.7-129.5 125.9 -18.1 20.8 -10.2 43 44 A V E +C 24 0A 18 159,-2.1 -19,-0.3 -2,-0.4 3,-0.1 -0.639 28.0 173.9 -74.7 123.5 -21.7 19.6 -10.2 44 45 A K E + 0 0 105 -21,-3.0 2,-0.3 -2,-0.4 -20,-0.2 0.688 63.5 18.5-102.7 -29.9 -23.8 22.0 -8.1 45 46 A E E S+C 23 0A 106 -22,-1.8 -22,-2.2 1,-0.1 -1,-0.3 -0.992 113.4 27.6-143.8 146.6 -27.3 20.7 -8.7 46 47 A G S S- 0 0 40 -2,-0.3 -1,-0.1 -24,-0.2 -22,-0.0 0.602 86.7-167.8 82.3 13.7 -28.9 17.5 -9.9 47 48 A G + 0 0 47 2,-0.1 -1,-0.2 1,-0.1 2,-0.1 -0.505 59.6 47.7 -86.5 158.0 -26.1 15.1 -8.8 48 49 A P S S- 0 0 88 0, 0.0 -1,-0.1 0, 0.0 153,-0.1 0.472 91.9-130.0 -75.0 137.0 -25.2 12.4 -9.1 49 50 A L - 0 0 5 151,-0.5 -2,-0.1 1,-0.1 4,-0.1 -0.282 10.0-151.6 -58.3 128.4 -25.6 12.9 -12.9 50 51 A P S S+ 0 0 64 0, 0.0 2,-0.3 0, 0.0 -1,-0.1 0.179 71.3 62.1 -87.2 19.7 -27.6 9.9 -14.3 51 52 A F S S- 0 0 5 79,-0.1 2,-0.3 80,-0.1 78,-0.2 -0.915 98.5 -74.4-140.9 164.7 -26.0 10.2 -17.8 52 53 A A > - 0 0 4 76,-1.5 3,-1.6 -2,-0.3 4,-0.2 -0.425 33.1-146.1 -62.8 120.9 -22.6 10.0 -19.5 53 54 A Y G >> S+ 0 0 7 1,-0.3 3,-2.5 -2,-0.3 4,-0.6 0.793 90.5 79.0 -59.3 -28.7 -20.6 13.1 -18.7 54 55 A D G >4 S+ 0 0 14 1,-0.3 3,-0.6 2,-0.2 4,-0.5 0.769 80.1 66.0 -52.8 -27.8 -19.0 12.8 -22.2 55 56 A I G <4 S+ 0 0 4 -3,-1.6 -1,-0.3 1,-0.2 -2,-0.2 0.686 105.1 45.9 -69.5 -14.7 -22.2 14.4 -23.8 56 57 A L G X> S+ 0 0 0 -3,-2.5 4,-1.3 -4,-0.2 3,-0.7 0.624 87.1 86.4 -95.8 -18.7 -21.3 17.7 -21.9 57 58 A T T << S+ 0 0 2 -3,-0.6 -2,-0.1 -4,-0.6 -1,-0.1 0.830 86.7 49.9 -68.1 -37.4 -17.6 18.1 -22.6 58 59 A M T 34 S+ 0 0 35 -4,-0.5 -1,-0.3 1,-0.2 -2,-0.1 0.788 106.2 59.4 -66.6 -27.7 -17.7 20.0 -25.9 59 60 A A T <4 0 0 2 -3,-0.7 -1,-0.2 1,-0.2 -2,-0.2 0.893 360.0 360.0 -66.4 -38.1 -20.2 22.4 -24.4 60 61 A F < 0 0 9 -4,-1.3 -2,-0.2 -3,-0.1 -1,-0.2 0.854 360.0 360.0 -71.2 360.0 -17.6 23.3 -21.7 61 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 62 65 A N > 0 0 8 0, 0.0 3,-1.9 0, 0.0 -3,-0.0 0.000 360.0 360.0 360.0 104.0 -14.6 28.3 -26.6 63 66 A R T 3 + 0 0 11 1,-0.3 3,-0.3 2,-0.0 14,-0.2 0.487 360.0 84.8 -76.3 -1.1 -12.8 28.5 -30.0 64 67 A V T 3 S+ 0 0 0 1,-0.3 2,-1.2 12,-0.2 -1,-0.3 0.812 83.2 67.6 -62.6 -26.7 -10.7 31.4 -28.8 65 68 A F S < S+ 0 0 1 -3,-1.9 2,-0.3 -30,-0.1 -1,-0.3 -0.533 80.2 102.1 -90.8 64.0 -8.6 28.4 -27.4 66 69 A A S S- 0 0 0 -2,-1.2 2,-0.8 -34,-0.6 -31,-0.3 -0.978 75.8-119.1-143.4 136.2 -7.5 27.1 -30.8 67 70 A K B -e 211 0A 103 143,-2.6 145,-3.0 -2,-0.3 -34,-0.1 -0.707 32.6-169.3 -79.5 110.8 -4.2 27.6 -32.5 68 71 A Y - 0 0 29 -2,-0.8 6,-0.1 143,-0.2 2,-0.1 -0.893 13.7-137.6-101.3 126.8 -4.7 29.5 -35.7 69 72 A P > - 0 0 48 0, 0.0 3,-1.5 0, 0.0 144,-0.0 -0.347 31.4-100.1 -74.7 169.2 -1.8 29.8 -38.2 70 73 A E T 3 S+ 0 0 210 1,-0.3 4,-0.1 -2,-0.1 -2,-0.0 0.720 116.7 59.0 -66.0 -25.1 -1.2 33.2 -39.9 71 74 A N T 3 S+ 0 0 130 2,-0.1 2,-0.4 1,-0.0 -1,-0.3 0.250 94.7 73.5 -94.5 12.5 -2.9 32.2 -43.2 72 75 A I S < S- 0 0 15 -3,-1.5 2,-0.2 111,-0.1 140,-0.1 -0.997 85.2-120.7-126.0 129.8 -6.3 31.5 -41.6 73 76 A V - 0 0 36 -2,-0.4 2,-0.9 109,-0.1 -2,-0.1 -0.469 22.0-139.3 -68.2 132.1 -8.5 34.3 -40.4 74 77 A D > - 0 0 25 -2,-0.2 4,-1.5 1,-0.2 107,-0.1 -0.807 21.2-179.4-102.4 95.5 -9.3 34.0 -36.7 75 78 A Y H > S+ 0 0 6 -2,-0.9 4,-0.7 1,-0.2 -1,-0.2 0.843 79.0 49.3 -57.9 -37.6 -13.0 34.8 -36.2 76 79 A F H >4 S+ 0 0 0 2,-0.2 3,-0.7 1,-0.2 4,-0.5 0.910 107.5 47.6 -81.3 -46.2 -12.9 34.3 -32.4 77 80 A K H >4 S+ 0 0 27 1,-0.2 3,-0.8 -14,-0.2 -1,-0.2 0.855 110.8 55.4 -66.3 -31.8 -9.8 36.2 -31.3 78 81 A Q H 3< S+ 0 0 65 -4,-1.5 -1,-0.2 1,-0.2 -2,-0.2 0.783 94.9 69.3 -64.7 -29.2 -11.1 39.2 -33.4 79 82 A S T - G 0 168A 4 4,-3.0 3,-2.1 -2,-0.7 76,-0.2 -0.530 24.3-118.4 -84.9 151.4 -26.8 11.0 -31.8 93 96 A E T 3 S+ 0 0 84 74,-2.3 75,-0.1 1,-0.3 -1,-0.1 0.700 112.4 54.8 -66.7 -19.1 -27.3 7.9 -34.0 94 97 A D T 3 S- 0 0 55 73,-0.2 -1,-0.3 2,-0.1 32,-0.0 0.222 126.9 -94.4 -98.8 14.6 -29.8 6.3 -31.6 95 98 A G S < S+ 0 0 37 -3,-2.1 2,-0.2 1,-0.4 -2,-0.1 0.317 78.7 138.5 96.1 -5.2 -32.1 9.4 -31.7 96 99 A G - 0 0 0 -5,-0.1 -4,-3.0 23,-0.1 2,-0.4 -0.517 33.0-168.0 -64.1 137.6 -30.9 11.3 -28.7 97 100 A I E -FH 91 120A 8 23,-2.4 23,-2.4 -6,-0.2 2,-0.4 -0.996 7.9-169.2-134.3 130.6 -30.6 15.0 -29.5 98 101 A C E -FH 90 119A 0 -8,-2.5 -8,-2.8 -2,-0.4 2,-0.3 -0.953 2.9-165.2-119.7 139.4 -29.0 17.8 -27.5 99 102 A N E +FH 89 118A 1 19,-3.0 19,-3.1 -2,-0.4 2,-0.3 -0.895 14.7 173.5-112.6 148.9 -29.2 21.5 -28.0 100 103 A A E -FH 88 117A 2 -12,-2.2 -12,-2.6 -2,-0.3 2,-0.3 -0.993 14.6-166.0-152.6 154.9 -26.8 24.0 -26.3 101 104 A T E -FH 87 116A 23 15,-2.0 15,-2.3 -2,-0.3 2,-0.4 -0.981 3.8-168.1-137.2 151.7 -26.0 27.6 -26.3 102 105 A N E -FH 86 115A 3 -16,-2.3 -16,-2.5 -2,-0.3 2,-0.5 -0.998 7.9-162.8-139.8 137.1 -23.0 29.6 -24.9 103 106 A D E -FH 85 114A 55 11,-1.4 11,-1.8 -2,-0.4 2,-0.5 -0.991 16.9-162.3-119.1 119.3 -22.6 33.3 -24.5 104 107 A I E +FH 84 113A 0 -20,-2.8 -20,-1.3 -2,-0.5 2,-0.2 -0.908 14.6 167.5-113.1 121.1 -18.9 34.3 -24.0 105 108 A T E - 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