==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=24-JUL-2010 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 19-FEB-10 3LVA . COMPND 2 MOLECULE: GREEN FLUORESCENT PROTEIN; . SOURCE 2 ORGANISM_SCIENTIFIC: AEQUOREA COERULESCENS; . AUTHOR N.V.PLETNEVA,V.Z.PLETNEV,S.V.PLETNEV . 468 4 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 19139.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 344 73.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 24 5.1 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 190 40.6 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 56 12.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 49 10.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 14 3.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 6 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 2 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 2 0 0 0 4 0 2 4 4 2 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 2 A S 0 0 65 0, 0.0 4,-0.3 0, 0.0 3,-0.1 0.000 360.0 360.0 360.0 162.0 -33.1 -9.8 10.6 2 3 A K > + 0 0 179 1,-0.2 3,-1.4 2,-0.2 4,-0.3 0.843 360.0 61.4 -69.9 -36.0 -32.5 -6.5 12.4 3 4 A G G > S+ 0 0 5 1,-0.3 3,-1.6 80,-0.2 -1,-0.2 0.805 91.2 69.0 -62.1 -26.7 -29.0 -7.6 13.5 4 5 A A G > S+ 0 0 25 1,-0.3 3,-1.7 2,-0.2 -1,-0.3 0.779 85.2 69.1 -63.3 -25.5 -28.0 -7.9 9.9 5 6 A E G X S+ 0 0 119 -3,-1.4 3,-0.9 -4,-0.3 -1,-0.3 0.730 88.2 65.6 -66.1 -20.5 -28.1 -4.1 9.6 6 7 A L G < S+ 0 0 76 -3,-1.6 -1,-0.3 -4,-0.3 3,-0.2 0.550 100.4 51.8 -75.9 -6.3 -25.1 -3.8 11.9 7 8 A F G < S+ 0 0 5 -3,-1.7 30,-0.4 -4,-0.2 29,-0.4 0.217 72.5 102.2-117.9 13.0 -23.0 -5.6 9.2 8 9 A T S < S+ 0 0 94 -3,-0.9 -1,-0.1 28,-0.1 2,-0.1 0.689 88.7 29.8 -74.4 -16.7 -23.7 -3.5 6.1 9 10 A G S S- 0 0 37 -3,-0.2 2,-0.5 -4,-0.1 27,-0.5 -0.338 110.0 -54.4-120.3-157.9 -20.3 -1.6 6.4 10 11 A I - 0 0 104 25,-0.1 25,-0.2 -2,-0.1 -2,-0.1 -0.776 53.8-162.4 -89.9 124.2 -16.8 -2.3 7.7 11 12 A V E -A 34 0A 9 23,-2.4 23,-2.2 -2,-0.5 104,-0.1 -0.903 16.0-125.0-112.6 131.3 -16.9 -3.4 11.3 12 13 A P E -A 33 0A 51 0, 0.0 104,-2.7 0, 0.0 2,-0.4 -0.431 28.6-154.1 -72.3 149.9 -13.9 -3.4 13.7 13 14 A I E -Ab 32 116A 4 19,-2.4 19,-1.7 102,-0.2 2,-0.4 -0.977 16.0-174.2-131.8 135.7 -13.0 -6.7 15.4 14 15 A L E -Ab 31 117A 80 102,-2.2 104,-2.2 -2,-0.4 2,-0.4 -0.992 8.5-172.5-124.9 139.9 -11.3 -7.7 18.6 15 16 A I E -Ab 30 118A 0 15,-2.6 15,-2.5 -2,-0.4 2,-0.4 -0.995 4.4-176.6-133.0 134.6 -10.4 -11.3 19.5 16 17 A E E -Ab 29 119A 68 102,-2.5 104,-2.9 -2,-0.4 2,-0.4 -0.994 5.9-179.6-133.8 130.6 -9.1 -12.6 22.8 17 18 A L E -Ab 28 120A 0 11,-2.7 11,-2.4 -2,-0.4 2,-0.5 -0.992 14.3-170.6-132.5 130.7 -8.1 -16.2 23.4 18 19 A N E -Ab 27 121A 65 102,-2.1 104,-2.7 -2,-0.4 2,-0.3 -0.986 22.3-179.6-110.9 129.9 -6.8 -18.1 26.4 19 20 A G E -Ab 26 122A 6 7,-2.8 7,-2.2 -2,-0.5 2,-0.3 -0.925 23.1-172.0-131.3 156.2 -5.7 -21.7 25.8 20 21 A D E +Ab 25 123A 48 102,-1.2 104,-3.3 -2,-0.3 2,-0.5 -0.886 10.8 179.6-146.8 106.0 -4.2 -24.6 27.6 21 22 A V E > S-Ab 24 124A 0 3,-2.7 3,-2.4 -2,-0.3 104,-0.2 -0.959 77.2 -23.5-117.1 115.1 -3.1 -27.6 25.6 22 23 A N T 3 S- 0 0 50 102,-2.4 -1,-0.2 -2,-0.5 103,-0.1 0.818 128.7 -51.1 51.5 34.8 -1.7 -30.5 27.5 23 24 A G T 3 S+ 0 0 56 101,-0.4 2,-0.7 1,-0.3 -1,-0.3 0.383 111.3 125.1 86.8 -5.1 -0.8 -28.0 30.2 24 25 A H E < -A 21 0A 74 -3,-2.4 -3,-2.7 100,-0.1 -1,-0.3 -0.817 47.7-158.1 -90.2 114.0 0.9 -25.6 27.8 25 26 A K E +A 20 0A 155 -2,-0.7 2,-0.3 -5,-0.2 -5,-0.2 -0.590 19.4 160.5 -98.6 150.7 -0.7 -22.3 28.2 26 27 A F E -A 19 0A 2 -7,-2.2 -7,-2.8 -2,-0.2 2,-0.4 -0.980 24.2-146.1-156.7 158.5 -0.9 -19.3 25.9 27 28 A S E -A 18 0A 28 -2,-0.3 21,-1.9 -9,-0.2 22,-0.8 -0.988 13.4-167.7-128.8 145.3 -2.9 -16.2 25.2 28 29 A V E -AC 17 47A 0 -11,-2.4 -11,-2.7 -2,-0.4 2,-0.4 -0.993 2.0-166.3-130.7 132.2 -3.6 -14.7 21.8 29 30 A S E -AC 16 46A 33 17,-2.3 17,-2.7 -2,-0.4 2,-0.3 -0.929 11.8-176.8-115.5 150.7 -5.0 -11.3 21.0 30 31 A G E +AC 15 45A 5 -15,-2.5 -15,-2.6 -2,-0.4 2,-0.3 -0.989 8.7 175.8-145.8 150.2 -6.3 -10.2 17.6 31 32 A E E +AC 14 44A 102 13,-1.9 13,-2.8 -2,-0.3 2,-0.3 -0.988 25.1 100.1-149.6 156.8 -7.7 -7.2 15.8 32 33 A G E -AC 13 43A 18 -19,-1.7 -19,-2.4 -2,-0.3 2,-0.3 -0.952 55.6 -76.5 167.0-139.0 -8.8 -6.2 12.3 33 34 A E E -AC 12 42A 99 9,-2.1 9,-2.2 -2,-0.3 2,-0.4 -0.988 20.5-142.4-155.0 158.1 -12.0 -5.7 10.4 34 35 A G E -AC 11 41A 1 -23,-2.2 -23,-2.4 -2,-0.3 2,-0.4 -0.949 3.2-166.9-126.4 147.6 -14.8 -7.6 8.7 35 36 A D E >>> + C 0 40A 31 5,-2.9 5,-2.0 -2,-0.4 3,-1.5 -0.772 7.8 178.5-135.5 82.2 -16.8 -6.7 5.6 36 37 A A T 345S+ 0 0 6 -27,-0.5 35,-0.2 -2,-0.4 -28,-0.1 0.715 75.4 72.1 -61.3 -25.5 -19.8 -9.0 5.4 37 38 A T T 345S+ 0 0 55 -30,-0.4 -1,-0.3 -28,-0.3 -29,-0.1 0.848 117.9 21.0 -59.1 -33.0 -21.1 -7.4 2.2 38 39 A Y T <45S- 0 0 75 -3,-1.5 404,-0.3 2,-0.1 -2,-0.2 0.481 109.7-116.5-111.4 -9.1 -18.2 -9.1 0.5 39 40 A G T <5 + 0 0 0 -4,-1.3 182,-2.4 1,-0.2 2,-0.3 0.734 63.6 149.9 79.9 25.3 -17.6 -11.9 3.0 40 41 A K E < -CD 35 220A 69 -5,-2.0 -5,-2.9 180,-0.2 2,-0.4 -0.718 26.2-176.8-102.8 141.9 -14.1 -10.6 3.8 41 42 A L E -CD 34 219A 15 178,-2.3 178,-2.3 -2,-0.3 2,-0.4 -0.992 4.9-175.7-130.8 138.4 -12.1 -10.7 7.0 42 43 A T E +CD 33 218A 61 -9,-2.2 -9,-2.1 -2,-0.4 2,-0.4 -0.966 18.8 163.7-134.2 119.3 -8.7 -9.3 7.8 43 44 A L E -CD 32 217A 5 174,-2.6 174,-3.5 -2,-0.4 2,-0.5 -0.996 26.2-155.7-141.3 139.8 -7.1 -10.1 11.2 44 45 A K E -CD 31 216A 48 -13,-2.8 -13,-1.9 -2,-0.4 2,-0.4 -0.970 15.9-166.5-115.8 132.1 -3.8 -9.9 12.9 45 46 A F E -CD 30 215A 3 170,-2.7 170,-2.1 -2,-0.5 2,-0.4 -0.919 4.1-170.4-118.4 139.0 -3.1 -12.2 15.9 46 47 A I E -CD 29 214A 12 -17,-2.7 -17,-2.3 -2,-0.4 2,-0.7 -0.978 25.1-128.0-123.9 141.8 -0.2 -12.0 18.4 47 48 A C E > -C 28 0A 6 166,-2.4 3,-0.7 -2,-0.4 166,-0.3 -0.810 20.7-168.8 -84.1 118.3 0.8 -14.5 21.0 48 49 A T T 3 S+ 0 0 87 -21,-1.9 -20,-0.1 -2,-0.7 -1,-0.1 0.481 80.0 61.1 -89.4 -2.7 1.0 -12.3 24.2 49 50 A T T 3 S- 0 0 76 -22,-0.8 2,-0.7 1,-0.2 -1,-0.2 0.107 113.3-107.8-108.1 20.0 2.7 -15.1 26.2 50 51 A G S < S+ 0 0 46 -3,-0.7 163,-0.5 -23,-0.1 2,-0.4 -0.845 88.0 27.1 100.0-115.9 5.8 -15.4 24.0 51 52 A K S S- 0 0 156 -2,-0.7 -3,-0.0 -3,-0.1 161,-0.0 -0.742 76.9-126.9 -88.5 129.7 6.0 -18.5 21.8 52 53 A L - 0 0 2 -2,-0.4 4,-0.1 1,-0.1 -26,-0.1 -0.601 14.5-152.0 -71.5 128.7 2.9 -20.2 20.8 53 54 A P S S+ 0 0 29 0, 0.0 83,-0.2 0, 0.0 -1,-0.1 0.503 76.3 46.7 -87.1 -2.9 3.2 -23.9 21.8 54 55 A V S S- 0 0 3 81,-0.1 2,-0.2 82,-0.1 80,-0.1 -0.832 100.9 -81.3-128.0 167.1 0.9 -25.1 19.0 55 56 A P >> - 0 0 9 0, 0.0 3,-1.2 0, 0.0 4,-0.6 -0.479 30.2-130.1 -71.8 138.1 0.7 -24.3 15.3 56 57 A W H >> S+ 0 0 12 1,-0.3 3,-1.9 -2,-0.2 4,-0.5 0.887 106.4 62.9 -49.6 -42.1 -1.2 -21.2 14.3 57 58 A P H >4 S+ 0 0 4 0, 0.0 3,-1.0 0, 0.0 4,-0.4 0.831 94.4 60.8 -59.0 -29.1 -3.2 -23.2 11.8 58 59 A T H <4 S+ 0 0 2 -3,-1.2 4,-0.2 1,-0.2 -2,-0.2 0.697 106.6 47.2 -68.2 -19.1 -4.8 -25.2 14.6 59 60 A L H 0 0 17 0, 0.0 3,-2.0 0, 0.0 -3,-0.0 0.000 360.0 360.0 360.0 61.6 -16.6 -14.7 13.7 66 69 A Q G > + 0 0 26 1,-0.3 3,-1.6 2,-0.2 16,-0.2 0.504 360.0 86.9 -71.2 -5.1 -18.9 -16.3 11.1 67 70 A C G 3 S+ 0 0 0 1,-0.3 -1,-0.3 14,-0.2 15,-0.1 0.623 80.7 67.1 -62.7 -14.1 -21.3 -13.3 11.4 68 71 A F G < S+ 0 0 1 -3,-2.0 -1,-0.3 -29,-0.1 153,-0.2 0.389 74.2 118.1 -91.0 6.5 -19.0 -11.8 8.7 69 72 A S S < S- 0 0 2 -3,-1.6 2,-0.7 1,-0.1 153,-0.2 -0.374 71.0-116.8 -64.8 145.1 -20.1 -14.4 6.1 70 73 A R B -e 222 0A 57 151,-2.6 153,-2.9 -32,-0.2 -33,-0.1 -0.780 27.6-165.7 -81.5 119.5 -21.7 -13.0 3.0 71 74 A Y - 0 0 5 -2,-0.7 5,-0.1 151,-0.2 153,-0.1 -0.937 21.8-127.7-100.6 120.5 -25.3 -14.4 2.7 72 75 A P > - 0 0 14 0, 0.0 3,-1.9 0, 0.0 4,-0.3 -0.251 28.7-102.8 -58.2 160.0 -26.7 -13.7 -0.8 73 76 A D G > S+ 0 0 132 1,-0.3 3,-1.5 2,-0.2 4,-0.3 0.875 121.9 55.0 -51.3 -45.4 -30.1 -12.0 -0.9 74 77 A H G 3 S+ 0 0 140 1,-0.3 -1,-0.3 2,-0.1 3,-0.1 0.599 114.3 42.1 -70.7 -8.1 -31.9 -15.2 -1.6 75 78 A M G X S+ 0 0 11 -3,-1.9 3,-2.4 1,-0.1 -1,-0.3 0.294 77.0 107.7-115.7 7.6 -30.4 -16.8 1.5 76 79 A K G X S+ 0 0 99 -3,-1.5 3,-1.2 -4,-0.3 -2,-0.1 0.755 71.4 66.6 -65.3 -19.8 -30.7 -13.9 4.0 77 80 A Q G 3 S+ 0 0 106 -4,-0.3 -1,-0.3 1,-0.2 -2,-0.1 0.541 98.4 56.1 -73.6 -6.0 -33.5 -15.7 5.9 78 81 A H G < S+ 0 0 22 -3,-2.4 2,-1.4 115,-0.1 -1,-0.2 0.315 73.6 111.4-108.8 8.6 -30.8 -18.4 6.8 79 82 A D <> + 0 0 7 -3,-1.2 4,-1.1 1,-0.2 112,-0.2 -0.636 31.2 164.8 -90.5 85.2 -28.3 -16.1 8.5 80 83 A F H > + 0 0 1 -2,-1.4 4,-1.4 1,-0.2 111,-0.2 0.899 67.8 63.5 -61.3 -40.7 -28.4 -17.1 12.2 81 84 A F H 4 S+ 0 0 0 1,-0.3 3,-0.4 2,-0.2 4,-0.2 0.899 107.7 37.7 -61.3 -49.9 -25.2 -15.4 13.1 82 85 A K H >4 S+ 0 0 12 1,-0.2 3,-1.5 2,-0.2 -1,-0.3 0.820 109.0 64.0 -68.7 -30.1 -26.2 -11.8 12.4 83 86 A S H 3< S+ 0 0 11 -4,-1.1 -80,-0.2 1,-0.3 -1,-0.2 0.788 96.9 57.9 -68.6 -25.2 -29.8 -12.3 13.7 84 87 A A T 3X S+ 0 0 4 -4,-1.4 4,-2.1 -3,-0.4 102,-0.3 0.546 94.5 144.6 -78.2 -9.3 -28.4 -12.9 17.2 85 88 A M T <4 + 0 0 4 -3,-1.5 -82,-0.1 1,-0.3 -81,-0.1 -0.514 48.8 15.0 -86.6 152.2 -26.7 -9.5 17.2 86 89 A P T 4 S+ 0 0 65 0, 0.0 25,-1.6 0, 0.0 -1,-0.3 -0.998 128.7 48.6 -85.0 -11.0 -26.0 -7.3 19.0 87 90 A E T 4 S- 0 0 99 1,-0.2 -2,-0.2 -3,-0.2 2,-0.1 0.837 103.3-154.2 -59.5 -34.1 -26.7 -9.4 22.1 88 91 A G < - 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0 0 1 -2,-0.2 -4,-2.4 -3,-0.1 2,-0.3 0.132 45.4-117.7 -82.0-169.6 -5.7 -35.0 20.8 102 105 A N E -FH 96 125A 14 23,-2.2 23,-2.0 -6,-0.2 2,-0.4 -0.962 6.8-142.0-134.6 148.2 -8.7 -32.7 20.9 103 106 A Y E -FH 95 124A 0 -8,-2.2 -8,-2.9 -2,-0.3 2,-0.4 -0.874 12.3-166.8-101.5 145.5 -9.5 -29.0 21.5 104 107 A K E -FH 94 123A 105 19,-2.5 19,-2.7 -2,-0.4 2,-0.3 -1.000 14.9-179.2-127.4 132.4 -12.7 -27.9 23.3 105 108 A S E -FH 93 122A 13 -12,-2.4 -12,-1.9 -2,-0.4 2,-0.4 -0.957 21.7-168.3-135.3 150.4 -13.7 -24.3 23.2 106 109 A R E +FH 92 121A 125 15,-2.2 15,-2.5 -2,-0.3 2,-0.3 -0.992 21.5 170.4-132.7 129.4 -16.3 -21.9 24.4 107 110 A A E -FH 91 120A 6 -16,-2.1 -16,-2.4 -2,-0.4 2,-0.4 -0.955 26.2-145.6-135.7 152.2 -16.6 -18.3 23.0 108 111 A E E -FH 90 119A 67 11,-2.0 11,-2.3 -2,-0.3 2,-0.5 -0.990 14.7-168.1-113.6 133.7 -18.9 -15.4 23.1 109 112 A V E +FH 89 118A 1 -20,-2.5 -20,-2.1 -2,-0.4 2,-0.3 -0.985 31.5 121.6-121.1 120.0 -19.3 -13.3 20.0 110 113 A K E - H 0 117A 52 7,-2.1 7,-2.9 -2,-0.5 2,-0.4 -0.983 61.7 -78.0-165.8 169.2 -21.1 -10.0 20.4 111 114 A F E - 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