==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=20-SEP-2013 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER IMMUNE SYSTEM 25-FEB-13 2M5H . COMPND 2 MOLECULE: MIP18 FAMILY PROTEIN FAM96A; . SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS; . AUTHOR B.OUYANG,B.XIA . 139 1 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 9262.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 91 65.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 9 6.5 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 10 7.2 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 1 0.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 1 0.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 1 0.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 1 0.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 6 4.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 9 6.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 50 36.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 2 1.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 1 1 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 27 A M 0 0 247 0, 0.0 2,-0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 154.9 1.5 1.8 7.5 2 28 A R - 0 0 198 1,-0.1 0, 0.0 0, 0.0 0, 0.0 -0.233 360.0 -98.1 -71.1 164.3 -0.7 0.8 4.5 3 29 A Q - 0 0 156 1,-0.1 -1,-0.1 -2,-0.0 2,-0.0 -0.528 45.6-106.0 -76.4 150.7 0.4 -0.5 1.1 4 30 A P - 0 0 95 0, 0.0 2,-0.2 0, 0.0 -1,-0.1 -0.246 40.4 -84.4 -74.5 165.6 0.6 2.0 -1.8 5 31 A R - 0 0 121 40,-0.0 2,-0.4 2,-0.0 40,-0.3 -0.467 39.6-159.7 -66.7 141.5 -1.8 2.3 -4.8 6 32 A I - 0 0 100 -2,-0.2 2,-1.0 38,-0.1 3,-0.3 -0.991 15.1-144.3-127.7 131.5 -1.2 -0.0 -7.8 7 33 A M > + 0 0 27 36,-0.9 4,-2.4 -2,-0.4 5,-0.2 -0.317 44.8 146.6 -91.5 48.8 -2.6 0.8 -11.3 8 34 A E H > S+ 0 0 122 -2,-1.0 4,-2.0 1,-0.2 -1,-0.2 0.906 71.4 45.1 -60.0 -46.1 -3.3 -2.9 -12.2 9 35 A E H > S+ 0 0 118 -3,-0.3 4,-2.6 2,-0.2 -1,-0.2 0.869 111.0 54.9 -69.0 -31.3 -6.4 -2.4 -14.4 10 36 A K H > S+ 0 0 70 2,-0.2 4,-3.2 1,-0.2 -2,-0.2 0.933 107.2 50.8 -63.9 -40.0 -4.7 0.6 -16.1 11 37 A A H X S+ 0 0 11 -4,-2.4 4,-2.5 2,-0.2 -2,-0.2 0.902 109.9 50.1 -62.4 -38.3 -1.9 -1.9 -17.0 12 38 A L H X S+ 0 0 80 -4,-2.0 4,-2.4 2,-0.2 -2,-0.2 0.930 113.6 45.2 -62.3 -45.8 -4.6 -4.3 -18.4 13 39 A E H X S+ 0 0 101 -4,-2.6 4,-2.1 2,-0.2 -2,-0.2 0.928 113.0 50.0 -63.9 -46.0 -6.0 -1.4 -20.5 14 40 A V H X S+ 0 0 1 -4,-3.2 4,-1.6 2,-0.2 -2,-0.2 0.906 111.1 50.6 -58.3 -44.2 -2.5 -0.3 -21.6 15 41 A Y H >X S+ 0 0 25 -4,-2.5 4,-2.5 -5,-0.2 3,-0.6 0.970 110.1 48.2 -57.6 -53.1 -1.8 -3.9 -22.6 16 42 A D H 3X S+ 0 0 84 -4,-2.4 4,-0.6 1,-0.2 -2,-0.2 0.810 106.8 58.8 -61.6 -29.0 -5.1 -4.2 -24.6 17 43 A L H 3< S+ 0 0 46 -4,-2.1 -1,-0.2 1,-0.2 3,-0.2 0.905 117.9 30.3 -60.6 -45.8 -4.2 -0.8 -26.4 18 44 A I H X< S+ 0 0 0 -4,-1.6 3,-1.9 -3,-0.6 12,-0.3 0.731 100.9 83.7 -88.3 -20.6 -0.9 -2.3 -27.7 19 45 A R H 3< S+ 0 0 79 -4,-2.5 12,-1.3 1,-0.3 13,-0.3 0.765 93.5 45.8 -51.7 -37.7 -2.1 -6.0 -28.1 20 46 A T T 3< S+ 0 0 35 -4,-0.6 -1,-0.3 -3,-0.2 2,-0.1 0.277 81.9 119.0 -98.4 4.9 -3.7 -5.3 -31.5 21 47 A I < - 0 0 1 -3,-1.9 9,-2.8 111,-0.1 10,-0.3 -0.481 61.4-128.2 -64.6 144.0 -0.8 -3.4 -33.1 22 48 A R B -A 29 0A 52 107,-2.6 7,-0.2 7,-0.3 6,-0.1 -0.647 21.1-101.5 -94.8 152.2 0.6 -5.1 -36.3 23 49 A D - 0 0 6 5,-1.8 -1,-0.1 3,-0.4 43,-0.0 -0.389 34.3-110.7 -66.2 148.4 4.3 -6.0 -37.0 24 50 A P S S+ 0 0 33 0, 0.0 -1,-0.1 0, 0.0 104,-0.0 0.691 114.9 14.9 -54.5 -22.9 6.3 -3.6 -39.5 25 51 A E S S+ 0 0 139 1,-0.1 -3,-0.0 3,-0.1 -2,-0.0 0.691 116.1 71.3-114.9 -49.3 6.4 -6.5 -42.1 26 52 A K - 0 0 95 1,-0.1 -3,-0.4 2,-0.1 -1,-0.1 -0.480 68.6-140.4 -79.2 144.2 3.8 -9.2 -41.0 27 53 A P S S+ 0 0 87 0, 0.0 -1,-0.1 0, 0.0 2,-0.1 0.631 78.5 91.8 -74.4 -13.8 -0.0 -8.6 -41.2 28 54 A N S S- 0 0 74 1,-0.1 -5,-1.8 -6,-0.1 -3,-0.1 -0.392 82.7 -98.4 -82.7 160.0 -0.5 -10.4 -37.8 29 55 A T B > -A 22 0A 12 -7,-0.2 4,-2.4 -2,-0.1 -7,-0.3 -0.304 27.1-107.8 -80.5 160.2 -0.6 -8.6 -34.3 30 56 A L T 4>S+ 0 0 1 -9,-2.8 5,-2.7 -12,-0.3 6,-0.6 0.802 122.0 52.4 -55.3 -34.1 2.2 -8.2 -31.7 31 57 A E T >45S+ 0 0 75 -12,-1.3 3,-1.4 -10,-0.3 -1,-0.2 0.932 109.5 47.5 -68.4 -45.2 0.3 -10.7 -29.5 32 58 A E T 345S+ 0 0 111 -13,-0.3 -2,-0.2 1,-0.3 -1,-0.2 0.868 112.8 49.6 -62.9 -36.7 0.2 -13.3 -32.3 33 59 A L T 3<5S- 0 0 59 -4,-2.4 -1,-0.3 -7,-0.1 -2,-0.2 0.399 114.2-123.7 -78.6 -1.7 3.9 -12.7 -33.0 34 60 A E T < 5S+ 0 0 178 -3,-1.4 -3,-0.2 -5,-0.2 -2,-0.1 0.674 70.5 134.1 66.8 22.5 4.5 -13.2 -29.1 35 61 A V < + 0 0 6 -5,-2.7 23,-1.4 -6,-0.2 2,-0.3 0.737 69.2 20.1 -70.4 -25.7 6.2 -9.6 -28.8 36 62 A V + 0 0 11 -6,-0.6 2,-0.2 21,-0.1 -2,-0.1 -0.908 56.1 169.9-145.8 162.4 4.1 -8.7 -25.7 37 63 A S > - 0 0 63 -2,-0.3 3,-1.5 1,-0.1 4,-0.5 -0.920 55.7 -80.8-163.3 175.2 2.1 -10.2 -22.7 38 64 A E G > S+ 0 0 128 1,-0.3 3,-2.1 -2,-0.2 -1,-0.1 0.932 127.2 56.3 -53.8 -47.5 0.5 -8.8 -19.4 39 65 A S G 3 S+ 0 0 116 1,-0.3 -1,-0.3 -3,-0.1 17,-0.1 0.628 95.3 68.1 -63.7 -13.6 3.9 -9.0 -17.6 40 66 A C G < S+ 0 0 15 -3,-1.5 16,-2.4 15,-0.1 2,-0.3 0.732 97.5 63.9 -71.4 -24.9 5.4 -6.8 -20.4 41 67 A V E < +B 55 0B 11 -3,-2.1 2,-0.3 -4,-0.5 14,-0.2 -0.831 55.6 167.6-112.8 140.4 3.3 -3.8 -19.1 42 68 A E E -B 54 0B 82 12,-2.6 12,-3.1 -2,-0.3 2,-0.3 -0.977 10.4-166.1-144.2 154.6 3.2 -1.9 -15.8 43 69 A V E -B 53 0B 1 -2,-0.3 -36,-0.9 10,-0.2 2,-0.3 -0.993 4.5-174.8-141.7 145.2 1.6 1.5 -14.6 44 70 A Q E -B 52 0B 101 8,-2.7 8,-2.5 -2,-0.3 2,-0.4 -0.965 28.2-110.3-137.4 154.0 2.1 3.7 -11.5 45 71 A E E +B 51 0B 61 -40,-0.3 6,-0.3 -2,-0.3 3,-0.1 -0.714 27.3 173.3 -83.3 132.7 0.4 6.9 -10.0 46 72 A I E S- 0 0 66 4,-2.6 2,-0.2 1,-0.6 5,-0.2 0.805 78.7 -22.0 -96.4 -49.2 2.5 10.2 -10.1 47 73 A N E > S-B 50 0B 97 3,-2.6 3,-1.9 0, 0.0 -1,-0.6 -0.896 80.6 -86.8-145.5 174.3 -0.5 12.3 -8.8 48 74 A E T 3 S+ 0 0 126 1,-0.3 3,-0.1 -2,-0.2 -3,-0.0 0.687 133.2 30.7 -65.7 -13.1 -4.4 11.7 -8.8 49 75 A E T 3 S+ 0 0 155 1,-0.1 36,-0.7 35,-0.0 2,-0.3 0.022 117.8 63.2-125.5 22.8 -4.4 13.3 -12.4 50 76 A E E < +Bc 47 85B 46 -3,-1.9 -3,-2.6 34,-0.1 -4,-2.6 -0.961 60.9 178.4-152.7 130.2 -0.9 12.2 -13.6 51 77 A Y E -Bc 45 86B 26 34,-2.2 36,-3.0 -2,-0.3 2,-0.4 -0.969 24.4-130.9-142.5 148.2 0.5 8.6 -14.2 52 78 A L E -Bc 44 87B 37 -8,-2.5 -8,-2.7 -2,-0.3 2,-0.4 -0.861 18.8-163.3-100.1 130.7 3.6 6.7 -15.3 53 79 A V E -Bc 43 88B 0 34,-3.2 36,-1.3 -2,-0.4 2,-0.4 -0.958 7.1-170.1-114.3 132.6 3.3 3.9 -17.9 54 80 A I E +Bc 42 89B 19 -12,-3.1 -12,-2.6 -2,-0.4 2,-0.4 -0.986 7.6 179.9-125.9 130.8 6.2 1.4 -18.4 55 81 A I E -Bc 41 90B 0 34,-2.3 36,-2.7 -2,-0.4 2,-0.5 -0.997 7.4-174.8-132.0 132.1 6.6 -1.3 -21.2 56 82 A R E + c 0 91B 170 -16,-2.4 36,-0.2 -2,-0.4 2,-0.2 -0.871 26.0 172.3-125.2 88.2 9.6 -3.7 -21.4 57 83 A F E - c 0 92B 3 34,-1.9 36,-0.6 -2,-0.5 -21,-0.1 -0.511 33.3-137.1-105.4 168.4 9.2 -5.7 -24.7 58 84 A T - 0 0 76 -23,-1.4 34,-0.0 -2,-0.2 -2,-0.0 -0.997 31.2-131.8-123.3 121.6 11.1 -8.2 -27.0 59 85 A P - 0 0 14 0, 0.0 8,-0.1 0, 0.0 9,-0.0 -0.163 18.8-112.7 -76.0 167.8 11.0 -7.6 -30.8 60 86 A T S S+ 0 0 44 1,-0.2 -37,-0.0 3,-0.1 -2,-0.0 0.824 105.3 13.4 -66.4 -33.5 10.2 -10.0 -33.7 61 87 A V S S- 0 0 49 2,-0.2 2,-1.6 33,-0.1 6,-0.2 -0.981 78.6-119.0-139.5 149.7 13.9 -9.7 -34.9 62 88 A P S S+ 0 0 57 0, 0.0 2,-0.4 0, 0.0 37,-0.1 -0.297 78.1 115.8 -81.6 49.4 17.0 -8.2 -33.2 63 89 A H >> - 0 0 108 -2,-1.6 4,-2.9 1,-0.1 3,-1.6 -0.989 68.5-135.3-129.1 130.6 17.3 -5.6 -36.0 64 90 A C H 3>>S+ 0 0 48 -2,-0.4 4,-2.2 1,-0.3 5,-0.5 0.808 99.6 67.0 -55.8 -36.9 17.0 -1.8 -35.6 65 91 A S H 345S+ 0 0 85 1,-0.2 -1,-0.3 2,-0.2 4,-0.1 0.736 120.6 19.1 -61.7 -26.5 14.7 -1.4 -38.7 66 92 A L H <>5S+ 0 0 31 -3,-1.6 4,-2.7 -5,-0.1 5,-0.2 0.750 121.2 59.7-105.2 -42.5 11.9 -3.3 -37.0 67 93 A A H X5S+ 0 0 0 -4,-2.9 4,-1.7 1,-0.2 -3,-0.2 0.878 109.3 44.2 -59.3 -41.9 12.9 -3.1 -33.3 68 94 A T H X5S+ 0 0 23 -4,-2.2 4,-2.6 -5,-0.2 -1,-0.2 0.939 114.4 49.9 -61.0 -48.1 12.8 0.8 -33.3 69 95 A L H >> S+ 0 0 67 0, 0.0 3,-2.1 0, 0.0 4,-0.8 0.165 100.6 83.3-175.2 -50.7 25.4 -2.2 -28.3 99 125 A T H >> S+ 0 0 36 1,-0.3 4,-2.5 2,-0.2 3,-0.7 0.795 84.0 67.3 -42.7 -44.4 22.5 -1.5 -30.8 100 126 A E H 3> S+ 0 0 35 1,-0.2 4,-2.4 2,-0.2 -1,-0.3 0.801 95.8 54.9 -48.9 -37.9 19.9 -2.1 -28.0 101 127 A E H <> S+ 0 0 143 -3,-2.1 4,-1.5 2,-0.2 -1,-0.2 0.863 110.9 44.6 -70.6 -35.6 21.1 1.0 -26.2 102 128 A D H S+ 0 0 172 -2,-0.4 4,-2.5 1,-0.2 5,-0.1 0.869 102.5 47.3 -57.6 -41.6 10.9 12.8 -27.2 111 137 A E H > S+ 0 0 144 2,-0.2 4,-2.7 1,-0.2 -1,-0.2 0.910 114.1 47.7 -70.0 -38.4 13.0 14.4 -30.0 112 138 A R H > S+ 0 0 98 2,-0.2 4,-2.3 -7,-0.2 -2,-0.2 0.921 112.5 49.5 -65.6 -40.6 13.8 11.0 -31.6 113 139 A V H X S+ 0 0 21 -4,-3.0 4,-1.3 2,-0.2 -2,-0.2 0.923 113.4 47.0 -62.0 -43.5 10.0 10.1 -31.3 114 140 A A H X S+ 0 0 44 -4,-2.5 4,-2.2 -5,-0.2 3,-0.3 0.930 110.7 51.6 -63.6 -44.3 9.2 13.5 -33.0 115 141 A A H X S+ 0 0 30 -4,-2.7 4,-2.3 1,-0.2 -2,-0.2 0.896 107.9 52.3 -60.6 -41.2 11.8 12.9 -35.7 116 142 A A H < S+ 0 0 10 -4,-2.3 7,-0.4 1,-0.2 -1,-0.2 0.814 112.2 46.4 -64.0 -31.6 10.3 9.5 -36.5 117 143 A M H < S+ 0 0 40 -4,-1.3 -2,-0.2 -3,-0.3 -1,-0.2 0.783 109.8 52.7 -81.9 -29.1 6.9 11.1 -36.9 118 144 A E H < S+ 0 0 156 -4,-2.2 -2,-0.2 -5,-0.1 -3,-0.2 0.893 92.3 87.1 -68.8 -39.3 8.2 14.0 -39.1 119 145 A N S X S- 0 0 68 -4,-2.3 4,-2.2 -5,-0.2 5,-0.2 -0.445 75.0-148.7 -60.8 124.7 9.8 11.3 -41.4 120 146 A P H > S+ 0 0 83 0, 0.0 4,-2.9 0, 0.0 5,-0.2 0.905 91.0 47.8 -70.5 -42.3 7.1 10.3 -44.0 121 147 A N H > S+ 0 0 96 2,-0.2 4,-2.3 1,-0.2 5,-0.2 0.950 115.7 43.5 -65.2 -49.2 8.1 6.7 -44.5 122 148 A L H > S+ 0 0 55 2,-0.2 4,-3.1 1,-0.2 5,-0.2 0.954 116.0 49.4 -63.0 -47.0 8.4 5.8 -40.8 123 149 A R H X S+ 0 0 103 -4,-2.2 4,-3.1 -7,-0.4 5,-0.2 0.949 110.9 49.2 -52.1 -55.6 5.1 7.7 -40.0 124 150 A E H X S+ 0 0 127 -4,-2.9 4,-1.7 1,-0.2 -1,-0.2 0.866 114.5 45.1 -59.8 -38.4 3.2 5.9 -42.9 125 151 A I H X S+ 0 0 43 -4,-2.3 4,-2.5 2,-0.2 -1,-0.2 0.962 113.9 49.5 -65.9 -49.6 4.4 2.5 -41.6 126 152 A V H X S+ 0 0 0 -4,-3.1 4,-3.0 2,-0.2 5,-0.3 0.908 109.2 52.2 -58.4 -43.8 3.6 3.4 -37.9 127 153 A E H X S+ 0 0 64 -4,-3.1 4,-1.6 1,-0.2 -1,-0.2 0.935 112.6 44.8 -58.0 -47.2 0.1 4.6 -38.9 128 154 A Q H < S+ 0 0 110 -4,-1.7 -1,-0.2 -5,-0.2 -2,-0.2 0.874 116.7 47.5 -64.1 -36.8 -0.6 1.2 -40.7 129 155 A C H < S+ 0 0 7 -4,-2.5 -107,-2.6 1,-0.2 -2,-0.2 0.904 111.7 47.3 -70.4 -44.7 1.0 -0.7 -37.7 130 156 A V H < S+ 0 0 12 -4,-3.0 2,-0.5 -109,-0.2 -2,-0.2 0.732 94.7 84.7 -76.3 -23.1 -0.9 1.1 -34.9 131 157 A L S < S- 0 0 91 -4,-1.6 -109,-0.2 -5,-0.3 -54,-0.0 -0.707 85.3-128.3 -79.0 123.9 -4.3 0.8 -36.7 132 158 A E - 0 0 94 -2,-0.5 2,-0.2 1,-0.1 -111,-0.1 -0.382 18.2-103.1 -75.8 149.9 -5.7 -2.7 -35.7 133 159 A P - 0 0 40 0, 0.0 -1,-0.1 0, 0.0 -104,-0.0 -0.484 21.4-152.4 -73.1 142.8 -7.0 -5.4 -38.2 134 160 A D - 0 0 143 -2,-0.2 -2,-0.0 0, 0.0 0, 0.0 0.932 67.5 -56.0 -81.3 -48.8 -10.8 -5.7 -38.5 135 161 A Q - 0 0 132 0, 0.0 3,-0.0 0, 0.0 0, 0.0 0.150 54.9-173.7-157.2 -70.2 -11.1 -9.4 -39.5 136 162 A L + 0 0 82 1,-0.2 2,-1.8 2,-0.1 3,-0.3 0.728 19.1 161.9 66.7 30.2 -9.1 -10.1 -42.7 137 163 A E + 0 0 148 1,-0.2 -1,-0.2 0, 0.0 0, 0.0 -0.140 21.4 133.3 -73.0 40.0 -10.4 -13.7 -43.0 138 164 A H 0 0 166 -2,-1.8 -1,-0.2 1,-0.2 -2,-0.1 0.568 360.0 360.0 -75.7 -11.4 -9.4 -13.9 -46.7 139 165 A H 0 0 252 -3,-0.3 -1,-0.2 0, 0.0 0, 0.0 -0.929 360.0 360.0-155.4 360.0 -7.7 -17.4 -46.4