==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=7-DEC-2009 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER BIOTIN-BINDING PROTEIN 08-AUG-02 1MEP . COMPND 2 MOLECULE: STREPTAVIDIN; . SOURCE 2 ORGANISM_SCIENTIFIC: STREPTOMYCES AVIDINII; . AUTHOR D.E.HYRE,I.LE TRONG,E.A.MERRITT,R.E.STENKAMP,N.M.GREEN, . 473 4 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 18686.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 326 68.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 241 51.0 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 1.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 4 0.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 54 11.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 25 5.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 2 0.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 5 1 0 4 4 7 1 1 7 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 16 A G 0 0 71 0, 0.0 86,-0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 -48.0 33.3 -14.5 25.9 2 17 A I + 0 0 9 16,-0.1 14,-0.2 79,-0.1 87,-0.0 0.814 360.0 101.3 -84.9 -30.2 29.7 -15.6 25.4 3 18 A T + 0 0 72 15,-0.1 2,-0.3 12,-0.1 14,-0.2 -0.315 66.4 47.2 -57.1 130.7 29.4 -17.5 28.6 4 19 A G E S-A 16 0A 28 12,-2.9 12,-2.8 2,-0.0 2,-0.4 -0.916 93.5 -33.0 141.2-164.0 27.5 -15.6 31.2 5 20 A T E -A 15 0A 57 -2,-0.3 2,-0.3 10,-0.2 10,-0.2 -0.812 51.4-170.9 -99.6 133.2 24.4 -13.5 31.8 6 21 A W E -A 14 0A 2 8,-2.9 8,-3.0 -2,-0.4 2,-0.3 -0.939 4.7-157.3-127.5 147.7 23.0 -11.3 29.1 7 22 A Y E -AB 13 116A 51 109,-3.1 109,-3.1 -2,-0.3 2,-0.2 -0.958 1.8-162.2-126.1 140.3 20.3 -8.6 29.1 8 23 A N > - 0 0 12 4,-1.5 3,-0.9 -2,-0.3 107,-0.1 -0.619 37.9-102.8-112.1 179.3 18.0 -7.1 26.4 9 24 A Q T 3 S+ 0 0 118 105,-0.3 106,-0.1 1,-0.2 -1,-0.0 0.651 115.4 65.7 -75.8 -18.9 16.0 -3.9 26.5 10 25 A L T 3 S- 0 0 70 2,-0.1 -1,-0.2 1,-0.0 3,-0.1 0.682 117.0-108.2 -81.2 -16.0 12.8 -5.7 27.1 11 26 A G S < S+ 0 0 49 -3,-0.9 -2,-0.1 1,-0.3 2,-0.1 0.454 72.6 138.1 103.2 2.6 14.0 -6.8 30.5 12 27 A S - 0 0 3 19,-0.0 -4,-1.5 16,-0.0 2,-0.4 -0.402 41.2-143.9 -81.5 165.2 14.5 -10.4 29.7 13 28 A T E -AC 7 29A 24 16,-2.4 16,-3.1 -6,-0.2 2,-0.5 -0.986 10.3-166.4-134.2 133.1 17.5 -12.3 30.9 14 29 A F E -AC 6 28A 1 -8,-3.0 -8,-2.9 -2,-0.4 2,-0.5 -0.986 0.9-167.2-126.4 124.0 19.5 -15.0 29.1 15 30 A I E +AC 5 27A 48 12,-2.6 12,-1.3 -2,-0.5 2,-0.4 -0.914 20.4 172.5-105.1 130.4 22.0 -17.2 30.9 16 31 A V E -AC 4 26A 1 -12,-2.8 -12,-2.9 -2,-0.5 2,-0.5 -0.995 28.2-155.7-145.2 148.3 24.3 -19.2 28.6 17 32 A T E - C 0 25A 71 8,-2.7 8,-1.9 -2,-0.4 2,-0.8 -0.984 16.0-149.2-117.2 127.7 27.3 -21.5 28.6 18 33 A A E - C 0 24A 22 -2,-0.5 6,-0.2 6,-0.2 -15,-0.1 -0.851 17.5-144.7 -99.0 107.7 29.4 -21.7 25.4 19 34 A G > - 0 0 25 4,-2.9 3,-0.7 -2,-0.8 26,-0.1 -0.140 21.3-114.0 -65.1 160.6 30.9 -25.2 25.1 20 35 A A T 3 S+ 0 0 100 1,-0.2 -1,-0.1 2,-0.1 -2,-0.0 0.768 115.8 49.0 -71.1 -21.4 34.3 -25.7 23.6 21 36 A D T 3 S- 0 0 102 25,-0.0 -1,-0.2 2,-0.0 26,-0.2 0.474 130.9 -84.3 -95.5 -10.7 32.9 -27.6 20.7 22 37 A G S < S+ 0 0 0 -3,-0.7 23,-1.9 1,-0.3 2,-0.2 0.414 78.2 140.3 120.7 1.8 30.2 -25.0 19.8 23 38 A A E - D 0 44A 24 21,-0.2 -4,-2.9 19,-0.0 2,-0.5 -0.490 34.1-158.9 -80.7 144.3 27.2 -25.8 22.0 24 39 A L E +CD 18 43A 1 19,-2.5 19,-2.0 -2,-0.2 2,-0.3 -0.981 21.3 169.5-110.5 124.7 25.1 -23.1 23.6 25 40 A T E +CD 17 42A 67 -8,-1.9 -8,-2.7 -2,-0.5 2,-0.3 -0.991 20.5 82.9-134.6 147.9 23.1 -24.3 26.7 26 41 A G E -CD 16 41A 20 15,-1.8 15,-2.6 -2,-0.3 2,-0.3 -0.987 63.1 -65.8 166.5-149.3 21.2 -22.4 29.3 27 42 A T E -CD 15 40A 34 -12,-1.3 -12,-2.6 -2,-0.3 2,-0.4 -0.992 26.1-148.6-138.4 152.2 17.9 -20.8 30.2 28 43 A Y E -CD 14 39A 6 11,-2.8 11,-2.2 -2,-0.3 2,-0.5 -0.961 3.9-160.9-118.2 137.7 16.0 -17.8 29.0 29 44 A E E -CD 13 38A 77 -16,-3.1 -16,-2.4 -2,-0.4 2,-0.2 -0.975 13.0-157.3-115.6 109.1 13.7 -15.6 31.2 30 45 A A - 0 0 17 7,-0.6 7,-0.1 -2,-0.5 3,-0.1 -0.567 13.2-168.7 -85.6 151.8 11.2 -13.6 29.1 31 46 A A S S+ 0 0 63 -2,-0.2 2,-0.3 1,-0.1 -1,-0.1 0.393 78.1 39.2-119.0 -2.5 9.8 -10.4 30.7 32 47 A V S S+ 0 0 42 431,-0.0 -1,-0.1 2,-0.0 2,-0.1 -0.961 94.7 46.3-134.1 156.4 7.1 -10.0 28.0 33 48 A G S S- 0 0 44 -2,-0.3 2,-2.2 1,-0.1 431,-0.0 -0.397 106.0 -45.5 104.2 170.6 5.1 -12.7 26.2 34 49 A N S S+ 0 0 86 -2,-0.1 2,-0.3 37,-0.1 37,-0.1 -0.559 101.6 118.4 -77.6 69.5 3.1 -15.7 27.4 35 50 A A + 0 0 26 -2,-2.2 2,-0.3 35,-0.1 3,-0.1 -0.999 39.6 177.2-147.7 142.8 6.0 -16.7 29.6 36 51 A E - 0 0 85 -2,-0.3 32,-0.2 2,-0.1 33,-0.2 -0.995 56.9 -9.1-146.9 144.7 6.8 -17.2 33.2 37 52 A S S S- 0 0 52 -2,-0.3 -7,-0.6 -7,-0.1 2,-0.2 -0.177 101.4 -34.7 64.3-163.5 10.0 -18.4 35.0 38 53 A R E -D 29 0A 134 -9,-0.2 2,-0.3 -3,-0.1 -9,-0.2 -0.520 52.3-157.0 -92.0 152.3 13.0 -19.7 33.1 39 54 A Y E -D 28 0A 1 -11,-2.2 -11,-2.8 -2,-0.2 2,-0.2 -0.969 25.9-105.7-130.3 145.3 13.0 -21.8 29.9 40 55 A V E -D 27 0A 65 25,-0.4 23,-0.6 -2,-0.3 2,-0.4 -0.482 37.0-169.8 -67.9 135.5 15.7 -24.1 28.5 41 56 A L E +DE 26 62A 1 -15,-2.6 -15,-1.8 -2,-0.2 2,-0.3 -0.947 8.0 177.5-124.1 151.5 17.6 -22.8 25.6 42 57 A T E +DE 25 61A 19 19,-1.9 19,-2.5 -2,-0.4 2,-0.3 -0.988 15.7 131.2-146.9 153.4 20.0 -24.6 23.3 43 58 A G E -DE 24 60A 0 -19,-2.0 -19,-2.5 -2,-0.3 2,-0.3 -0.898 40.5-103.1-175.1-165.9 21.9 -23.5 20.3 44 59 A R E -DE 23 59A 19 15,-1.6 15,-2.2 -2,-0.3 2,-0.3 -0.949 21.2-168.2-143.4 153.4 25.3 -23.4 18.6 45 60 A Y E - E 0 58A 30 -23,-1.9 2,-0.7 -2,-0.3 13,-0.2 -0.966 39.6 -94.6-142.9 157.5 28.0 -20.9 18.0 46 61 A D - 0 0 1 11,-2.5 -24,-0.1 -2,-0.3 148,-0.0 -0.625 37.8-170.6 -67.7 107.8 31.0 -20.6 15.7 47 62 A S S S+ 0 0 30 -2,-0.7 -1,-0.2 1,-0.3 -25,-0.1 0.398 80.5 45.3 -93.8 9.3 33.7 -21.8 18.2 48 63 A A S S+ 0 0 45 -26,-0.0 -1,-0.3 0, 0.0 3,-0.1 -0.498 85.9 175.9-137.5 70.4 36.6 -20.8 15.9 49 64 A P - 0 0 48 0, 0.0 7,-0.1 0, 0.0 2,-0.1 -0.102 39.0 -81.3 -67.9 171.6 35.6 -17.4 14.8 50 65 A A - 0 0 32 5,-0.4 3,-0.2 1,-0.1 169,-0.0 -0.309 28.5-152.1 -59.1 149.8 37.5 -14.9 12.7 51 66 A T S S+ 0 0 144 1,-0.1 -1,-0.1 -3,-0.1 4,-0.0 0.374 74.7 96.9-110.4 1.5 40.0 -12.9 14.6 52 67 A D S S- 0 0 104 3,-0.1 -1,-0.1 1,-0.1 3,-0.1 0.126 114.1 -88.3 -79.9 11.2 39.7 -9.9 12.2 53 68 A G S S+ 0 0 60 1,-0.3 168,-0.2 -3,-0.2 166,-0.1 0.221 86.8 139.6 94.6 -11.1 37.3 -8.1 14.5 54 69 A S - 0 0 22 164,-0.1 -1,-0.3 166,-0.1 166,-0.3 -0.255 57.0-102.7 -62.1 150.5 34.3 -9.9 12.9 55 70 A G - 0 0 5 164,-2.5 2,-0.6 162,-0.1 -5,-0.4 -0.108 30.8-108.0 -68.7 173.8 31.5 -11.0 15.2 56 71 A T E - F 0 81A 17 25,-2.2 25,-2.9 -7,-0.1 -1,-0.1 -0.950 32.9-132.7-106.8 112.7 31.0 -14.6 16.3 57 72 A A E + F 0 80A 1 -2,-0.6 -11,-2.5 23,-0.3 2,-0.3 -0.337 40.4 148.5 -69.0 147.7 27.9 -16.2 14.6 58 73 A L E -EF 45 79A 1 21,-2.2 21,-2.5 -13,-0.2 2,-0.3 -0.978 22.2-170.2-164.7 168.3 25.5 -18.0 16.9 59 74 A G E -EF 44 78A 0 -15,-2.2 -15,-1.6 -2,-0.3 2,-0.3 -0.968 3.4-171.1-160.7 158.8 21.9 -18.9 17.4 60 75 A W E -EF 43 77A 0 17,-1.9 17,-2.6 -2,-0.3 2,-0.3 -0.973 11.2-141.4-151.0 162.5 19.6 -20.5 19.9 61 76 A T E -EF 42 76A 0 -19,-2.5 -19,-1.9 -2,-0.3 2,-0.4 -0.955 6.3-168.4-124.4 142.2 16.1 -21.8 20.4 62 77 A V E -EF 41 75A 0 13,-2.2 13,-2.5 -2,-0.3 2,-0.6 -0.990 10.1-156.3-126.5 118.7 13.6 -21.6 23.2 63 78 A A E - F 0 74A 3 -23,-0.6 2,-2.3 -2,-0.4 11,-0.2 -0.884 26.3-130.5 -88.6 124.2 10.5 -23.7 23.2 64 79 A W E S+ 0 0 19 9,-2.6 7,-2.3 -2,-0.6 2,-0.4 -0.186 72.8 106.7 -80.8 50.5 8.1 -21.8 25.4 65 80 A K E + F 0 70A 60 -2,-2.3 -25,-0.4 5,-0.3 5,-0.2 -0.995 35.3 144.0-123.2 127.8 7.2 -24.8 27.5 66 81 A N - 0 0 22 3,-2.9 3,-0.1 -2,-0.4 -27,-0.1 -0.761 67.2 -70.7-142.3-166.2 8.4 -25.3 31.1 67 82 A N S S+ 0 0 157 -2,-0.2 3,-0.1 1,-0.2 -30,-0.1 0.758 128.4 45.1 -65.1 -17.5 7.0 -26.7 34.3 68 83 A Y S S- 0 0 143 1,-0.3 2,-0.3 -32,-0.2 -1,-0.2 0.753 121.3 -14.9-102.6 -25.1 4.7 -23.7 34.7 69 84 A R - 0 0 92 -33,-0.2 -3,-2.9 -3,-0.1 2,-0.3 -0.991 45.7-135.7-168.1 173.4 3.2 -23.2 31.3 70 85 A N E -F 65 0A 36 -2,-0.3 -5,-0.3 -5,-0.2 -35,-0.1 -0.948 8.2-176.4-143.0 114.6 3.2 -23.8 27.6 71 86 A A E - 0 0 21 -7,-2.3 -6,-0.1 -2,-0.3 3,-0.1 0.379 42.1-128.5 -90.3 -0.6 2.7 -21.1 25.0 72 87 A H E S+ 0 0 58 1,-0.2 26,-2.4 -8,-0.2 2,-0.3 0.946 74.1 101.2 45.0 61.8 2.8 -23.7 22.2 73 88 A S E - G 0 97A 9 24,-0.3 -9,-2.6 104,-0.1 2,-0.3 -0.980 56.4-149.4-162.6 164.1 5.4 -21.6 20.5 74 89 A A E -FG 63 96A 0 22,-2.7 22,-2.7 -2,-0.3 2,-0.4 -0.998 4.7-154.9-145.5 140.2 9.2 -21.3 19.9 75 90 A T E -FG 62 95A 6 -13,-2.5 -13,-2.2 -2,-0.3 2,-0.4 -0.943 5.0-165.6-115.4 141.0 11.6 -18.5 19.3 76 91 A T E -FG 61 94A 0 18,-2.1 18,-2.0 -2,-0.4 2,-0.5 -0.986 5.5-158.1-118.3 136.3 14.9 -18.7 17.5 77 92 A W E -FG 60 93A 8 -17,-2.6 -17,-1.9 -2,-0.4 2,-0.4 -0.957 7.9-172.1-109.0 129.8 17.5 -15.9 17.8 78 93 A S E +FG 59 92A 0 14,-3.2 14,-2.5 -2,-0.5 2,-0.3 -0.976 32.1 103.4-117.7 130.5 20.1 -15.6 15.1 79 94 A G E -FG 58 91A 1 -21,-2.5 -21,-2.2 -2,-0.4 2,-0.3 -0.910 56.8 -89.2-171.2-158.2 22.9 -13.2 15.5 80 95 A Q E -FG 57 90A 3 10,-2.1 10,-2.2 -2,-0.3 2,-0.4 -0.999 24.9-130.1-141.2 142.6 26.6 -12.5 16.2 81 96 A Y E -FG 56 89A 33 -25,-2.9 -25,-2.2 -2,-0.3 2,-0.5 -0.721 18.2-165.4 -83.6 137.8 28.5 -11.8 19.4 82 97 A V E - 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