==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=24-JUL-2010 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER BIOTIN-BINDING PROTEIN 05-APR-10 3MG5 . COMPND 2 MOLECULE: STREPTAVIDIN; . SOURCE 2 ORGANISM_SCIENTIFIC: STREPTOMYCES AVIDINII; . AUTHOR I.LE TRONG,L.BAUGH,P.S.STAYTON,T.P.LYBRAND,R.E.STENKAMP . 479 4 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 19739.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 326 68.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 242 50.5 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 1.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 4 0.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 56 11.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 24 5.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 2 4 0 5 3 8 0 1 7 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 16 A G 0 0 85 0, 0.0 86,-0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 -20.5 33.1 -14.2 26.6 2 17 A I + 0 0 9 79,-0.1 14,-0.2 16,-0.1 2,-0.0 0.851 360.0 98.0 -80.5 -38.5 29.7 -15.6 25.9 3 18 A T + 0 0 73 15,-0.1 2,-0.3 12,-0.1 14,-0.2 -0.297 69.4 40.3 -59.9 131.9 29.2 -17.5 29.1 4 19 A G E S-A 16 0A 22 12,-3.0 12,-2.4 2,-0.0 2,-0.4 -0.897 93.9 -31.1 134.0-163.2 27.2 -15.7 31.8 5 20 A T E -A 15 0A 48 -2,-0.3 2,-0.3 10,-0.2 10,-0.2 -0.802 49.9-179.7 -99.5 131.9 24.2 -13.5 32.3 6 21 A W E -A 14 0A 0 8,-2.9 8,-2.7 -2,-0.4 2,-0.4 -0.942 12.7-150.0-128.7 154.5 23.0 -11.0 29.7 7 22 A Y E -AB 13 116A 49 109,-2.5 109,-2.6 -2,-0.3 6,-0.2 -0.965 10.1-149.3-127.2 137.5 20.1 -8.6 29.7 8 23 A N E > - B 0 115A 12 4,-1.9 3,-1.4 -2,-0.4 107,-0.2 -0.421 34.9 -95.8-102.5 178.4 18.0 -7.4 26.7 9 24 A Q T 3 S+ 0 0 95 105,-0.6 106,-0.1 1,-0.3 104,-0.0 0.596 121.1 55.1 -70.2 -12.9 16.2 -4.1 26.0 10 25 A L T 3 S- 0 0 53 2,-0.1 -1,-0.3 22,-0.0 457,-0.0 0.476 121.1-101.6 -95.0 -5.7 12.8 -5.6 27.2 11 26 A G S < S+ 0 0 52 -3,-1.4 -2,-0.1 1,-0.3 2,-0.1 0.467 74.7 143.2 95.7 4.5 14.1 -6.6 30.6 12 27 A S - 0 0 2 18,-0.1 -4,-1.9 1,-0.0 2,-0.4 -0.376 43.0-134.3 -75.7 159.4 14.6 -10.3 29.8 13 28 A T E -AC 7 29A 31 16,-2.5 16,-2.4 -6,-0.2 2,-0.5 -0.979 13.3-166.6-126.5 122.6 17.5 -12.4 31.2 14 29 A F E -AC 6 28A 0 -8,-2.7 -8,-2.9 -2,-0.4 2,-0.5 -0.964 5.3-174.2-109.7 116.6 19.6 -14.8 29.1 15 30 A I E +AC 5 27A 44 12,-2.8 12,-2.2 -2,-0.5 2,-0.3 -0.974 18.7 166.8-117.2 116.3 21.8 -17.1 31.2 16 31 A V E -AC 4 26A 3 -12,-2.4 -12,-3.0 -2,-0.5 2,-0.4 -0.971 32.2-154.1-137.1 150.0 24.1 -19.2 29.0 17 32 A T E - C 0 25A 70 8,-2.6 8,-1.8 -2,-0.3 2,-0.7 -0.997 16.4-150.6-120.7 126.6 27.1 -21.4 29.1 18 33 A A E - C 0 24A 24 -2,-0.4 6,-0.2 6,-0.2 -15,-0.1 -0.891 16.2-148.1 -97.6 112.0 29.2 -21.7 25.9 19 34 A G > - 0 0 26 4,-2.9 3,-1.7 -2,-0.7 26,-0.1 -0.441 24.1-111.3 -80.5 158.8 30.9 -25.1 25.7 20 35 A A T 3 S+ 0 0 101 1,-0.3 -1,-0.1 2,-0.1 -2,-0.0 0.720 115.1 53.8 -61.2 -26.2 34.3 -25.6 24.1 21 36 A D T 3 S- 0 0 104 2,-0.0 -1,-0.3 25,-0.0 26,-0.2 0.503 128.6 -86.5 -86.0 -11.8 32.8 -27.5 21.2 22 37 A G S < S+ 0 0 0 -3,-1.7 23,-2.1 1,-0.3 2,-0.2 0.445 79.9 141.6 113.3 0.7 30.2 -24.9 20.1 23 38 A A E - D 0 44A 31 21,-0.2 -4,-2.9 19,-0.0 2,-0.4 -0.557 33.1-161.8 -76.9 141.6 27.3 -25.8 22.5 24 39 A L E +CD 18 43A 1 19,-2.3 19,-2.2 -2,-0.2 2,-0.3 -0.994 18.8 165.3-116.7 129.7 25.1 -23.2 24.1 25 40 A T E +CD 17 42A 77 -8,-1.8 -8,-2.6 -2,-0.4 2,-0.3 -0.990 20.8 84.7-137.4 149.7 23.0 -24.3 27.1 26 41 A G E -CD 16 41A 17 15,-1.6 15,-2.1 -2,-0.3 2,-0.3 -0.985 62.4 -60.2 161.6-147.8 21.1 -22.3 29.8 27 42 A T E -CD 15 40A 37 -12,-2.2 -12,-2.8 -2,-0.3 2,-0.4 -0.952 26.1-154.0-138.5 151.1 17.9 -20.5 30.6 28 43 A Y E -CD 14 39A 4 11,-2.4 11,-2.4 -2,-0.3 2,-0.5 -0.989 4.4-170.0-126.6 137.8 15.8 -17.7 29.3 29 44 A E E -C 13 0A 75 -16,-2.4 -16,-2.5 -2,-0.4 2,-0.4 -0.977 14.8-150.8-129.1 113.1 13.4 -15.5 31.3 30 45 A S - 0 0 24 7,-0.5 7,-0.1 -2,-0.5 5,-0.1 -0.720 14.0-158.8 -88.1 133.8 11.2 -13.2 29.3 31 46 A A S S+ 0 0 60 -2,-0.4 2,-0.3 5,-0.1 -1,-0.1 0.588 80.0 39.6 -78.8 -10.8 9.9 -9.9 30.7 32 47 A V S S+ 0 0 34 2,-0.0 -2,-0.1 435,-0.0 433,-0.0 -0.842 91.5 25.0-135.2 165.6 7.0 -10.0 28.1 33 48 A G S S- 0 0 43 -2,-0.3 2,-1.9 1,-0.1 435,-0.0 -0.240 105.9 -25.5 80.5-164.3 4.6 -12.3 26.4 34 49 A N S S+ 0 0 91 37,-0.1 2,-0.3 -2,-0.0 37,-0.2 -0.563 102.1 110.1 -87.9 71.3 3.1 -15.6 27.5 35 50 A A - 0 0 19 -2,-1.9 2,-0.3 -5,-0.1 3,-0.1 -0.997 40.5-177.7-151.1 142.6 5.9 -16.6 29.7 36 51 A E - 0 0 75 -2,-0.3 32,-0.2 33,-0.1 33,-0.1 -0.991 57.0 -18.4-146.9 138.2 6.7 -17.0 33.4 37 52 A S S S- 0 0 51 -2,-0.3 -7,-0.5 -7,-0.1 2,-0.3 -0.166 101.9 -25.4 65.0-162.4 9.9 -17.9 35.1 38 53 A R - 0 0 133 -9,-0.2 2,-0.3 -11,-0.1 -9,-0.2 -0.603 53.0-163.1 -89.5 149.7 12.9 -19.6 33.4 39 54 A Y E -D 28 0A 2 -11,-2.4 -11,-2.4 -2,-0.3 2,-0.1 -0.949 28.3-102.9-127.4 147.5 12.8 -21.8 30.2 40 55 A V E -D 27 0A 67 25,-0.4 23,-0.6 -2,-0.3 2,-0.3 -0.422 37.6-170.7 -66.0 136.0 15.4 -24.1 28.9 41 56 A L E +DE 26 62A 0 -15,-2.1 -15,-1.6 21,-0.1 2,-0.3 -0.971 8.5 178.7-124.3 150.2 17.5 -22.9 25.9 42 57 A T E +DE 25 61A 25 19,-1.5 19,-2.5 -2,-0.3 2,-0.3 -0.984 17.7 132.1-141.6 150.3 19.9 -24.8 23.7 43 58 A G E -DE 24 60A 1 -19,-2.2 -19,-2.3 -2,-0.3 2,-0.3 -0.899 39.3-102.4-169.0-161.8 21.9 -23.6 20.7 44 59 A R E -DE 23 59A 30 15,-2.0 15,-2.2 -2,-0.3 2,-0.3 -0.980 20.6-171.3-142.5 151.8 25.2 -23.4 18.9 45 60 A Y E - E 0 58A 32 -23,-2.1 2,-0.8 -2,-0.3 13,-0.2 -0.965 38.9 -94.4-140.6 160.7 28.0 -20.9 18.4 46 61 A D - 0 0 2 11,-2.6 -24,-0.1 -2,-0.3 148,-0.0 -0.637 33.7-168.8 -73.0 108.2 31.1 -20.6 16.3 47 62 A S S S+ 0 0 36 -2,-0.8 -1,-0.2 1,-0.3 -25,-0.1 0.449 82.3 42.3 -83.9 2.4 33.8 -21.9 18.5 48 63 A A S S+ 0 0 45 -26,-0.0 -1,-0.3 0, 0.0 3,-0.1 -0.483 83.0 174.7-142.1 65.4 36.7 -20.7 16.3 49 64 A P - 0 0 43 0, 0.0 7,-0.1 0, 0.0 6,-0.1 -0.116 41.2 -80.1 -68.1 166.4 35.6 -17.2 15.1 50 65 A A - 0 0 38 5,-0.4 3,-0.2 1,-0.1 142,-0.0 -0.274 31.8-143.8 -57.4 154.8 37.6 -14.8 13.0 51 66 A T S S+ 0 0 147 1,-0.1 -1,-0.1 -3,-0.1 4,-0.0 0.156 77.4 92.5-112.2 14.8 40.2 -12.8 14.9 52 67 A D S S- 0 0 107 3,-0.1 -1,-0.1 2,-0.0 3,-0.1 0.239 113.0 -86.7 -95.1 9.8 39.8 -9.4 13.0 53 68 A G S S+ 0 0 60 1,-0.3 168,-0.1 -3,-0.2 30,-0.1 0.236 87.0 135.8 95.3 -13.9 37.2 -7.8 15.3 54 69 A S - 0 0 21 1,-0.1 -1,-0.3 166,-0.1 166,-0.3 -0.259 59.2 -96.0 -57.3 156.2 34.2 -9.5 13.5 55 70 A G - 0 0 5 164,-2.4 2,-0.6 162,-0.1 -5,-0.4 -0.125 28.2-112.4 -75.7 166.8 31.5 -10.9 15.6 56 71 A T E - F 0 81A 16 25,-2.6 25,-2.6 -7,-0.1 2,-0.1 -0.915 33.5-130.2-102.4 117.3 31.0 -14.5 16.7 57 72 A A E + F 0 80A 1 -2,-0.6 -11,-2.6 23,-0.2 2,-0.3 -0.384 40.4 151.0 -71.9 140.7 28.0 -16.1 15.0 58 73 A L E -EF 45 79A 0 21,-2.3 21,-2.7 -13,-0.2 2,-0.3 -0.980 20.4-173.8-157.6 167.5 25.5 -17.9 17.3 59 74 A G E -EF 44 78A 0 -15,-2.2 -15,-2.0 -2,-0.3 2,-0.3 -0.977 2.5-172.9-163.0 159.9 21.9 -18.9 17.8 60 75 A W E -EF 43 77A 0 17,-1.9 17,-2.6 -2,-0.3 2,-0.4 -0.978 12.4-138.8-152.7 162.9 19.6 -20.5 20.3 61 76 A T E -EF 42 76A 0 -19,-2.5 -19,-1.5 -2,-0.3 2,-0.4 -0.966 7.2-169.6-126.5 141.4 16.0 -21.8 20.7 62 77 A V E -EF 41 75A 0 13,-2.5 13,-2.5 -2,-0.4 2,-0.5 -0.999 9.7-157.6-123.5 124.5 13.5 -21.6 23.5 63 78 A A E - F 0 74A 3 -23,-0.6 2,-2.1 -2,-0.4 11,-0.3 -0.927 28.7-127.0 -91.8 125.4 10.3 -23.7 23.4 64 79 A W E S+ 0 0 15 9,-2.5 7,-2.6 -2,-0.5 2,-0.4 -0.267 73.4 107.4 -80.1 52.8 7.9 -21.8 25.7 65 80 A K E + F 0 70A 59 -2,-2.1 -25,-0.4 5,-0.3 5,-0.2 -0.998 34.9 146.7-125.5 127.4 7.0 -24.7 27.9 66 81 A N - 0 0 24 3,-2.4 3,-0.2 -2,-0.4 -27,-0.1 -0.699 66.0 -72.6-139.3-166.1 8.3 -25.1 31.4 67 82 A N S S+ 0 0 158 -2,-0.2 3,-0.1 1,-0.2 -28,-0.1 0.671 128.8 44.8 -64.7 -20.2 6.9 -26.5 34.7 68 83 A Y S S- 0 0 150 1,-0.3 2,-0.3 -32,-0.2 -1,-0.2 0.651 121.1 -16.4-102.4 -30.1 4.5 -23.6 35.1 69 84 A R - 0 0 91 -3,-0.2 -3,-2.4 -33,-0.1 2,-0.4 -0.982 45.8-133.1-169.7 171.1 3.0 -23.1 31.7 70 85 A N E -F 65 0A 39 -2,-0.3 -5,-0.3 -5,-0.2 -35,-0.1 -0.953 8.8-176.6-141.2 116.2 3.0 -23.8 27.9 71 86 A A E - 0 0 19 -7,-2.6 27,-0.1 -2,-0.4 -6,-0.1 0.435 41.9-127.2 -86.6 -5.9 2.5 -21.1 25.3 72 87 A H E S+ 0 0 60 -8,-0.3 26,-2.8 1,-0.2 2,-0.3 0.951 74.4 102.2 54.7 52.4 2.6 -23.6 22.4 73 88 A S E - G 0 97A 9 24,-0.3 -9,-2.5 104,-0.1 2,-0.3 -0.958 54.9-151.8-156.4 163.6 5.3 -21.5 20.7 74 89 A A E -FG 63 96A 0 22,-2.2 22,-2.7 -2,-0.3 2,-0.4 -0.989 5.1-155.6-145.8 140.8 9.1 -21.3 20.1 75 90 A T E -FG 62 95A 5 -13,-2.5 -13,-2.5 -2,-0.3 2,-0.4 -0.957 4.6-165.2-116.4 137.7 11.5 -18.5 19.6 76 91 A T E -FG 61 94A 0 18,-2.2 18,-2.2 -2,-0.4 2,-0.4 -0.979 5.1-158.8-114.8 138.6 14.9 -18.7 17.8 77 92 A W E -FG 60 93A 6 -17,-2.6 -17,-1.9 -2,-0.4 2,-0.4 -0.976 7.5-173.5-115.6 130.2 17.5 -16.0 18.0 78 93 A S E +FG 59 92A 0 14,-2.8 14,-2.4 -2,-0.4 2,-0.3 -0.989 32.8 101.5-121.6 127.8 20.2 -15.7 15.3 79 94 A G E -FG 58 91A 1 -21,-2.7 -21,-2.3 -2,-0.4 2,-0.3 -0.877 56.4 -82.1-172.3-155.5 23.0 -13.2 15.8 80 95 A Q E -FG 57 90A 10 10,-2.1 10,-2.5 -2,-0.3 2,-0.4 -0.999 21.7-133.1-144.4 142.3 26.5 -12.4 16.7 81 96 A Y E -FG 56 89A 42 -25,-2.6 -25,-2.6 -2,-0.3 2,-0.5 -0.789 18.8-167.3 -88.6 132.7 28.5 -11.7 19.9 82 97 A V E - 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