==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=8-DEC-2009 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER ELECTRON TRANSPORT 28-NOV-02 1NAQ . COMPND 2 MOLECULE: PERIPLASMIC DIVALENT CATION TOLERANCE PROTEIN . SOURCE 2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI; . AUTHOR V.CALDERONE,S.MANGANI,M.BENVENUTI,M.S.VIEZZOLI,L.BANCI, . 628 6 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 25375.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 477 76.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 215 34.2 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 6 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 35 5.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 38 6.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 171 27.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 6 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 4 2 5 0 6 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 1 5 2 8 2 0 0 6 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 7 A S 0 0 133 0, 0.0 2,-0.2 0, 0.0 493,-0.1 0.000 360.0 360.0 360.0 114.8 22.8 16.1 47.2 2 8 A N - 0 0 53 1,-0.1 2,-0.2 3,-0.0 620,-0.1 -0.478 360.0-145.7 -83.7 139.3 25.2 13.9 45.2 3 9 A T - 0 0 10 -2,-0.2 99,-0.1 95,-0.2 2,-0.1 -0.519 35.3 -91.0 -90.3 161.0 25.6 10.1 45.4 4 10 A A - 0 0 11 -2,-0.2 60,-2.3 1,-0.1 61,-0.3 -0.435 43.3-133.0 -67.0 149.1 28.8 8.3 45.0 5 11 A S E -A 63 0A 7 58,-0.3 85,-1.0 613,-0.2 2,-0.3 -0.619 19.3-166.4 -98.2 163.5 29.7 7.2 41.4 6 12 A V E -AB 62 89A 10 56,-2.3 56,-1.7 -2,-0.2 2,-0.5 -0.967 18.7-128.5-140.7 157.7 31.0 3.8 40.1 7 13 A V E -AB 61 88A 3 81,-2.3 81,-3.7 -2,-0.3 2,-0.5 -0.963 20.1-162.5-107.9 122.7 32.5 2.4 36.9 8 14 A V E -AB 60 87A 0 52,-2.6 52,-3.4 -2,-0.5 2,-0.5 -0.964 3.4-157.1-104.9 119.9 30.9 -0.6 35.5 9 15 A L E +AB 59 86A 21 77,-3.0 77,-1.9 -2,-0.5 2,-0.4 -0.853 19.2 168.1 -94.0 124.7 33.0 -2.7 33.0 10 16 A C E -AB 58 85A 4 48,-1.7 48,-2.9 -2,-0.5 2,-0.4 -0.985 25.3-135.5-136.1 145.7 31.0 -4.8 30.6 11 17 A T E -A 57 0A 26 73,-0.7 73,-0.4 -2,-0.4 46,-0.2 -0.896 19.7-178.6-106.3 141.0 32.2 -6.7 27.5 12 18 A A E -A 56 0A 7 44,-2.4 44,-3.3 -2,-0.4 3,-0.1 -0.895 29.0-130.8-131.1 151.6 30.3 -6.8 24.1 13 19 A P S S- 0 0 80 0, 0.0 2,-0.3 0, 0.0 44,-0.1 0.802 81.0 -2.0 -76.7 -27.6 31.3 -8.6 20.9 14 20 A D S > S- 0 0 74 42,-0.1 4,-2.3 1,-0.1 5,-0.2 -0.933 73.8 -96.2-159.5 171.0 30.9 -5.7 18.4 15 21 A E H > S+ 0 0 55 -2,-0.3 4,-3.0 1,-0.2 5,-0.2 0.911 117.3 54.0 -68.6 -40.7 29.9 -2.1 18.0 16 22 A A H > S+ 0 0 72 2,-0.2 4,-1.8 1,-0.2 -1,-0.2 0.918 113.5 42.7 -60.6 -47.7 26.3 -2.8 16.9 17 23 A T H > S+ 0 0 63 2,-0.2 4,-2.4 1,-0.2 -2,-0.2 0.936 116.4 47.7 -59.5 -50.5 25.6 -4.9 20.1 18 24 A A H X S+ 0 0 0 -4,-2.3 4,-2.8 1,-0.2 -2,-0.2 0.884 112.6 48.5 -63.3 -41.2 27.4 -2.5 22.4 19 25 A Q H X S+ 0 0 56 -4,-3.0 4,-2.5 -5,-0.2 -1,-0.2 0.893 111.9 48.2 -71.2 -36.5 25.7 0.6 21.0 20 26 A D H X S+ 0 0 97 -4,-1.8 4,-2.0 -5,-0.2 -2,-0.2 0.920 113.8 48.9 -66.8 -40.2 22.2 -1.0 21.2 21 27 A L H X S+ 0 0 24 -4,-2.4 4,-2.4 2,-0.2 5,-0.2 0.958 111.9 47.3 -65.1 -51.8 22.9 -2.0 24.8 22 28 A A H X S+ 0 0 0 -4,-2.8 4,-2.4 1,-0.2 5,-0.3 0.917 109.8 53.3 -53.2 -49.3 24.2 1.4 25.8 23 29 A A H X S+ 0 0 29 -4,-2.5 4,-2.4 -5,-0.2 -1,-0.2 0.920 111.1 47.0 -55.8 -41.9 21.2 3.2 24.2 24 30 A K H X S+ 0 0 81 -4,-2.0 4,-1.6 2,-0.2 -2,-0.2 0.943 113.2 45.8 -69.7 -48.3 18.7 0.9 26.2 25 31 A V H <>S+ 0 0 0 -4,-2.4 5,-2.7 1,-0.2 6,-0.7 0.892 115.4 49.1 -64.4 -34.0 20.4 1.3 29.6 26 32 A L H ><5S+ 0 0 7 -4,-2.4 3,-1.6 -5,-0.2 -2,-0.2 0.907 108.9 51.2 -65.8 -44.2 20.7 5.1 29.0 27 33 A A H 3<5S+ 0 0 59 -4,-2.4 -2,-0.2 1,-0.3 -1,-0.2 0.804 110.8 50.6 -67.3 -27.1 17.0 5.4 28.0 28 34 A E T 3<5S- 0 0 96 -4,-1.6 -1,-0.3 -5,-0.2 -2,-0.2 0.411 112.6-122.4 -89.7 1.1 16.1 3.6 31.2 29 35 A K T < 5S+ 0 0 86 -3,-1.6 -3,-0.2 2,-0.2 -2,-0.1 0.862 74.5 126.6 58.6 44.7 18.3 5.9 33.3 30 36 A L S -C 49 0A 121 3,-2.5 3,-1.3 -2,-0.5 -2,-0.0 -0.979 69.0 -4.3-136.9 123.0 37.0 -22.2 27.9 47 53 A E T 3 S- 0 0 169 -2,-0.4 -1,-0.1 1,-0.3 3,-0.0 0.938 132.5 -63.2 41.4 70.2 36.0 -25.8 27.9 48 54 A G T 3 S+ 0 0 76 1,-0.1 2,-0.4 -3,-0.1 -1,-0.3 0.151 114.5 108.9 -92.8 80.5 39.3 -26.2 28.4 49 55 A K E < S- C 0 46A 122 -3,-1.3 -3,-2.5 -5,-0.1 2,-0.5 -0.983 73.3-105.9-117.6 141.1 40.7 -24.8 25.1 50 56 A L E - C 0 45A 49 -2,-0.4 2,-0.3 -5,-0.2 -5,-0.2 -0.491 43.2-167.7 -66.8 108.3 42.7 -21.5 24.7 51 57 A E E - C 0 44A 60 -7,-2.8 -7,-1.5 -2,-0.5 2,-0.4 -0.720 14.6-165.8 -98.6 153.6 40.3 -19.0 23.0 52 58 A Q E + C 0 43A 84 -2,-0.3 2,-0.4 -9,-0.2 -9,-0.2 -0.995 19.6 173.2-135.7 125.8 41.1 -15.6 21.5 53 59 A E E - C 0 42A 97 -11,-2.6 -11,-3.3 -2,-0.4 2,-0.3 -0.992 32.4-117.8-143.5 143.9 38.3 -13.4 20.7 54 60 A Y E + C 0 41A 188 -2,-0.4 2,-0.3 -13,-0.2 -13,-0.2 -0.566 40.8 175.1 -77.7 140.0 37.6 -9.8 19.5 55 61 A E E - C 0 40A 33 -15,-2.6 -15,-1.4 -2,-0.3 2,-0.5 -0.883 34.6-133.4-142.1 160.2 35.7 -7.6 21.9 56 62 A V E -A 12 0A 7 -44,-3.3 -44,-2.4 -2,-0.3 2,-0.5 -0.998 29.8-139.4-116.0 122.1 34.4 -4.1 22.5 57 63 A Q E -AC 11 37A 11 -20,-2.5 -20,-3.1 -2,-0.5 2,-0.4 -0.754 16.2-155.0 -83.8 118.6 35.2 -2.9 26.0 58 64 A M E -AC 10 36A 0 -48,-2.9 -48,-1.7 -2,-0.5 2,-0.5 -0.823 9.3-167.0 -98.0 137.6 32.3 -1.0 27.6 59 65 A I E -AC 9 35A 20 -24,-2.9 -24,-2.4 -2,-0.4 2,-0.5 -0.953 12.1-169.6-119.7 105.5 32.9 1.5 30.4 60 66 A L E -AC 8 34A 0 -52,-3.4 -52,-2.6 -2,-0.5 2,-0.5 -0.852 7.3-155.7-101.9 131.6 29.5 2.5 32.0 61 67 A K E +AC 7 33A 6 -28,-2.8 -29,-2.8 -2,-0.5 -28,-1.2 -0.893 32.2 134.0-109.9 125.7 29.3 5.4 34.5 62 68 A T E -A 6 0A 0 -56,-1.7 -56,-2.3 -2,-0.5 2,-0.3 -0.822 49.3 -87.0-147.5-177.3 26.5 5.4 37.0 63 69 A T E >> -A 5 0A 3 -58,-0.2 3,-1.2 -2,-0.2 4,-1.0 -0.714 33.9-113.8 -97.0 161.2 25.7 6.0 40.8 64 70 A V G >4 S+ 0 0 5 -60,-2.3 3,-0.7 -2,-0.3 4,-0.5 0.892 116.7 62.0 -56.7 -38.7 25.8 3.6 43.7 65 71 A S G 34 S+ 0 0 37 -61,-0.3 -1,-0.3 1,-0.2 4,-0.2 0.788 111.7 36.0 -59.4 -26.0 22.0 3.9 44.0 66 72 A H G <> S+ 0 0 45 -3,-1.2 4,-2.2 1,-0.1 -1,-0.2 0.439 89.5 98.5-106.9 -5.6 21.5 2.5 40.4 67 73 A Q H S+ 0 0 14 -4,-0.5 4,-2.7 2,-0.2 -1,-0.2 0.954 112.2 48.8 -62.8 -45.9 22.2 -3.2 41.2 69 75 A A H > S+ 0 0 44 -4,-0.2 4,-2.6 1,-0.2 -2,-0.2 0.910 112.2 50.4 -60.7 -39.5 19.5 -2.4 38.6 70 76 A L H X S+ 0 0 0 -4,-2.2 4,-2.5 -40,-0.2 5,-0.2 0.926 110.9 48.4 -60.8 -50.0 22.2 -1.8 35.9 71 77 A L H X S+ 0 0 9 -4,-2.6 4,-2.5 1,-0.2 -2,-0.2 0.943 112.2 49.8 -55.8 -50.0 23.9 -5.1 36.8 72 78 A E H X S+ 0 0 89 -4,-2.7 4,-2.0 1,-0.2 -2,-0.2 0.867 111.2 48.1 -56.1 -50.7 20.5 -6.9 36.6 73 79 A C H X S+ 0 0 17 -4,-2.6 4,-1.7 2,-0.2 -1,-0.2 0.898 112.9 47.3 -53.6 -49.2 19.6 -5.3 33.2 74 80 A L H X S+ 0 0 5 -4,-2.5 4,-1.1 1,-0.2 -2,-0.2 0.925 111.9 50.7 -65.5 -41.6 22.9 -6.2 31.6 75 81 A K H >< S+ 0 0 44 -4,-2.5 3,-0.8 -5,-0.2 -1,-0.2 0.936 109.3 51.8 -57.5 -46.5 22.7 -9.7 32.9 76 82 A S H 3< S+ 0 0 87 -4,-2.0 -1,-0.2 1,-0.3 -2,-0.2 0.778 108.8 50.5 -64.1 -30.6 19.2 -10.1 31.5 77 83 A H H 3< S+ 0 0 95 -4,-1.7 -1,-0.3 -5,-0.1 -2,-0.2 0.725 86.4 103.5 -79.4 -19.0 20.3 -9.0 28.1 78 84 A H S X< S- 0 0 53 -4,-1.1 3,-1.6 -3,-0.8 -3,-0.0 -0.353 76.7-128.7 -74.4 140.5 23.3 -11.4 27.8 79 85 A P T 3 S+ 0 0 108 0, 0.0 -1,-0.1 0, 0.0 -4,-0.0 0.697 110.0 27.1 -67.4 -18.9 22.6 -14.4 25.6 80 86 A Y T 3 S- 0 0 187 -5,-0.1 -2,-0.1 0, 0.0 -4,-0.1 0.146 108.9-116.0-118.2 11.8 23.8 -16.8 28.3 81 87 A Q S < S+ 0 0 94 -3,-1.6 120,-0.2 -6,-0.2 -6,-0.1 0.782 89.8 105.2 53.6 32.4 22.9 -14.6 31.2 82 88 A T + 0 0 64 119,-0.1 -1,-0.1 -7,-0.1 -7,-0.1 -0.414 47.8 178.0-141.8 69.4 26.6 -14.4 32.1 83 89 A P - 0 0 1 0, 0.0 2,-1.5 0, 0.0 119,-0.2 -0.235 41.1-113.8 -71.9 146.5 28.2 -11.0 31.1 84 90 A E + 0 0 28 -73,-0.4 -73,-0.7 118,-0.1 2,-0.5 -0.717 60.5 163.5 -80.0 94.2 31.8 -10.3 31.9 85 91 A L E +B 10 0A 0 -2,-1.5 114,-2.1 -75,-0.2 2,-0.3 -0.959 16.8 167.2-134.4 121.3 30.8 -7.6 34.3 86 92 A L E -BF 9 198A 4 -77,-1.9 -77,-3.0 -2,-0.5 2,-0.4 -0.956 21.8-143.6-136.0 145.8 32.9 -5.9 37.0 87 93 A V E -BF 8 197A 0 110,-2.5 109,-2.3 -2,-0.3 110,-0.9 -0.912 12.5-165.4-115.2 131.3 32.4 -2.8 39.2 88 94 A L E -B 7 0A 6 -81,-3.7 -81,-2.3 -2,-0.4 107,-0.2 -0.951 21.4-121.3-117.5 136.0 35.2 -0.5 40.2 89 95 A P E -B 6 0A 7 0, 0.0 2,-0.5 0, 0.0 -83,-0.2 -0.412 16.8-148.0 -75.7 143.5 34.9 2.1 43.0 90 96 A V - 0 0 5 -85,-1.0 208,-0.2 208,-0.1 -85,-0.2 -0.947 11.7-174.2-108.0 126.8 35.5 5.8 42.2 91 97 A T - 0 0 24 206,-2.4 2,-0.3 -2,-0.5 441,-0.2 0.559 52.6 -36.5 -98.7 -6.1 37.0 7.8 45.0 92 98 A H B +G 297 0A 28 205,-0.8 205,-3.2 439,-0.1 -1,-0.3 -0.952 56.3 86.0-174.7 162.9 36.8 11.3 43.4 93 99 A G + 0 0 2 203,-0.3 5,-0.1 -2,-0.3 203,-0.1 -0.679 50.3 57.4-169.9 -89.3 36.7 13.9 41.6 94 100 A D > - 0 0 11 201,-0.4 4,-2.6 -2,-0.2 5,-0.2 0.279 46.3-159.0 -50.7 118.5 35.2 16.6 39.3 95 101 A T H > S+ 0 0 76 1,-0.2 4,-2.1 2,-0.2 -1,-0.1 0.821 87.7 50.5 -70.7 -32.0 32.2 17.9 41.2 96 102 A D H > S+ 0 0 82 2,-0.2 4,-1.7 1,-0.2 -1,-0.2 0.904 111.0 48.9 -72.7 -38.1 30.4 19.3 38.2 97 103 A Y H > S+ 0 0 19 196,-0.2 4,-2.4 1,-0.2 -2,-0.2 0.928 112.4 48.8 -62.3 -46.6 30.7 16.1 36.3 98 104 A L H X S+ 0 0 26 -4,-2.6 4,-1.7 1,-0.2 -2,-0.2 0.872 107.3 54.5 -61.8 -39.9 29.5 14.3 39.4 99 105 A S H X S+ 0 0 49 -4,-2.1 4,-2.1 1,-0.2 -1,-0.2 0.836 110.5 48.3 -60.3 -34.2 26.5 16.6 39.7 100 106 A W H X S+ 0 0 52 -4,-1.7 4,-2.4 2,-0.2 -2,-0.2 0.872 105.8 54.6 -75.5 -38.1 25.6 15.9 36.1 101 107 A L H X S+ 0 0 1 -4,-2.4 4,-0.8 1,-0.2 -2,-0.2 0.948 113.0 45.7 -64.5 -29.9 25.8 12.1 36.5 102 108 A N H >< S+ 0 0 20 -4,-1.7 3,-0.8 1,-0.2 4,-0.5 0.929 111.4 48.8 -72.9 -48.1 23.4 12.5 39.4 103 109 A A H >< S+ 0 0 72 -4,-2.1 3,-0.9 1,-0.2 -1,-0.2 0.851 104.1 64.2 -57.2 -36.9 20.9 14.9 37.6 104 110 A S H 3< S+ 0 0 30 -4,-2.4 -1,-0.2 1,-0.2 -2,-0.2 0.835 106.4 42.1 -56.7 -36.5 21.0 12.4 34.6 105 111 A L T << 0 0 2 -3,-0.8 -1,-0.2 -4,-0.8 -2,-0.2 0.361 360.0 360.0-100.0 -2.9 19.4 9.6 36.7 106 112 A R < 0 0 229 -3,-0.9 -1,-0.2 -4,-0.5 -2,-0.2 0.001 360.0 360.0 155.7 360.0 16.7 11.6 38.6 107 !* 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 108 9 B T 0 0 56 0, 0.0 2,-0.3 0, 0.0 99,-0.1 0.000 360.0 360.0 360.0 116.2 36.4 -15.2 46.1 109 10 B A - 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0 0 10 -114,-1.9 4,-2.3 -2,-0.3 5,-0.2 -0.443 40.6-131.7 -53.1 131.2 29.1 14.1 53.9 622 101 F T H > S+ 0 0 79 1,-0.2 4,-1.3 2,-0.2 -1,-0.1 0.857 103.2 46.5 -63.7 -39.2 29.7 17.2 51.8 623 102 F D H > S+ 0 0 79 -120,-0.2 4,-1.5 2,-0.2 -1,-0.2 0.885 112.2 50.4 -71.5 -37.7 29.5 19.7 54.7 624 103 F Y H > S+ 0 0 12 -119,-0.2 4,-2.6 1,-0.2 3,-0.2 0.939 109.9 50.9 -61.5 -44.9 31.8 17.6 56.9 625 104 F L H X S+ 0 0 21 -4,-2.3 4,-2.8 1,-0.3 -1,-0.2 0.835 103.9 58.7 -67.5 -29.1 34.4 17.3 54.1 626 105 F S H X S+ 0 0 56 -4,-1.3 4,-2.8 -5,-0.2 -1,-0.3 0.923 109.9 43.2 -61.4 -41.4 34.3 21.1 53.6 627 106 F W H X S+ 0 0 66 -4,-1.5 4,-2.6 -3,-0.2 -2,-0.2 0.903 110.4 55.1 -73.7 -39.6 35.4 21.5 57.2 628 107 F L H < S+ 0 0 1 -4,-2.6 -2,-0.2 1,-0.2 -1,-0.2 0.967 113.7 43.5 -56.0 -47.4 37.9 18.8 56.9 629 108 F N H < S+ 0 0 29 -4,-2.8 4,-0.4 1,-0.2 -2,-0.2 0.946 109.4 53.2 -59.8 -54.4 39.4 20.7 54.0 630 109 F A H >< S+ 0 0 67 -4,-2.8 3,-0.8 3,-0.4 -1,-0.2 0.849 102.4 69.4 -56.3 -36.0 39.2 24.2 55.6 631 110 F S T 3< S+ 0 0 28 -4,-2.6 -72,-0.1 2,-0.4 -163,-0.0 -0.410 97.9 39.5 -62.8 155.8 41.1 22.6 58.4 632 111 F L T 3 0 0 17 1,-0.3 -1,-0.2 -79,-0.2 -2,-0.2 0.238 360.0 360.0 85.0 -25.3 44.6 21.9 56.9 633 112 F R < 0 0 198 -3,-0.8 -2,-0.4 -4,-0.4 -3,-0.4 0.484 360.0 360.0-138.1 360.0 43.4 24.7 56.0