==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=13-NOV-2011 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER MEMBRANE PROTEIN 20-OCT-06 2NLJ . COMPND 2 MOLECULE: ANTIBODY FAB FRAGMENT LIGHT CHAIN; . SOURCE 2 ORGANISM_SCIENTIFIC: MUS MUSCULUS; . AUTHOR S.W.LOCKLESS,M.ZHOU,R.MACKINNON . 533 3 3 3 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 24130.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 361 67.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 10 1.9 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 166 31.1 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 5 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 2 0.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 4 0.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 1 0.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 55 10.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 35 6.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 81 15.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 6 1.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 2 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 RESIDUES PER ALPHA HELIX . 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 5 7 6 3 3 4 3 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 4 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 A D 0 0 117 0, 0.0 2,-0.6 0, 0.0 95,-0.1 0.000 360.0 360.0 360.0 150.2 153.9 139.1 -12.5 2 2 A I - 0 0 7 93,-0.5 2,-0.4 91,-0.2 88,-0.1 -0.752 360.0-144.0 -89.7 121.8 151.0 141.4 -11.8 3 3 A L - 0 0 111 -2,-0.6 23,-2.0 94,-0.0 2,-0.6 -0.685 9.6-162.5 -85.9 133.8 150.0 141.5 -8.1 4 4 A L E -A 25 0A 6 -2,-0.4 96,-0.7 94,-0.4 2,-0.5 -0.934 7.4-162.2-119.0 104.9 146.2 141.8 -7.4 5 5 A T E -A 24 0A 62 19,-2.9 19,-2.5 -2,-0.6 2,-0.6 -0.791 6.8-170.6 -95.6 124.4 145.5 143.0 -3.9 6 6 A Q - 0 0 6 -2,-0.5 17,-0.2 17,-0.2 -2,-0.0 -0.926 20.3-158.2-115.2 106.4 142.0 142.4 -2.5 7 7 A S + 0 0 79 -2,-0.6 2,-0.1 15,-0.3 15,-0.1 -1.000 65.9 37.0-142.0 137.5 141.5 144.2 0.8 8 8 A P S S- 0 0 71 0, 0.0 14,-0.2 0, 0.0 -1,-0.1 0.520 72.2-153.0 -71.2 156.8 139.7 144.0 3.2 9 9 A A S S+ 0 0 63 -2,-0.1 94,-2.6 1,-0.1 2,-0.4 0.731 80.2 35.9 -71.1 -25.9 139.6 140.2 3.0 10 10 A I E -d 103 0B 96 92,-0.2 2,-0.5 75,-0.0 94,-0.2 -0.999 68.5-159.7-134.0 135.4 136.2 140.0 4.7 11 11 A L E -d 104 0B 35 92,-2.5 94,-2.7 -2,-0.4 2,-0.7 -0.963 2.7-160.1-117.7 123.2 133.2 142.4 4.2 12 12 A S E +d 105 0B 79 -2,-0.5 2,-0.3 92,-0.2 94,-0.2 -0.888 24.1 163.9-104.6 111.6 130.4 142.6 6.8 13 13 A V E -d 106 0B 6 92,-2.6 94,-3.4 -2,-0.7 3,-0.1 -0.876 34.7-119.1-126.0 158.2 127.3 144.2 5.3 14 14 A S > - 0 0 47 -2,-0.3 3,-2.5 92,-0.2 64,-0.3 -0.734 47.5 -84.4 -95.8 146.2 123.6 144.3 6.4 15 15 A P T 3 S+ 0 0 71 0, 0.0 64,-0.2 0, 0.0 -1,-0.1 -0.148 116.5 18.8 -46.9 133.6 120.9 142.8 4.2 16 16 A G T 3 S+ 0 0 48 62,-1.8 61,-0.3 1,-0.3 63,-0.1 0.197 96.3 124.1 89.1 -18.5 119.8 145.3 1.6 17 17 A E < - 0 0 96 -3,-2.5 61,-2.2 60,-0.2 2,-0.6 -0.165 67.5-106.9 -72.3 170.5 122.9 147.5 1.8 18 18 A R - 0 0 141 59,-0.2 2,-0.3 58,-0.2 58,-0.2 -0.886 38.3-163.3 -99.8 120.9 125.2 148.4 -1.1 19 19 A V E - B 0 75A 4 56,-2.3 56,-2.4 -2,-0.6 2,-0.4 -0.825 7.4-160.1-110.1 148.8 128.6 146.6 -0.9 20 20 A S E - B 0 74A 61 -2,-0.3 2,-0.4 54,-0.2 54,-0.2 -0.944 1.6-160.3-133.1 114.7 131.8 147.4 -2.7 21 21 A F E - B 0 73A 1 52,-3.4 52,-2.2 -2,-0.4 2,-0.4 -0.736 17.0-143.5 -91.7 138.5 134.8 144.9 -3.3 22 22 A S E - B 0 72A 42 -2,-0.4 2,-0.4 -14,-0.2 -15,-0.3 -0.807 18.1-178.6-109.2 142.1 138.2 146.4 -4.0 23 23 A a E - B 0 71A 0 48,-2.4 48,-3.0 -2,-0.4 2,-0.5 -0.954 5.0-172.7-139.0 116.9 140.9 145.2 -6.4 24 24 A R E -AB 5 70A 125 -19,-2.5 -19,-2.9 -2,-0.4 2,-0.2 -0.944 10.5-151.7-114.8 121.3 144.2 147.0 -6.7 25 25 A A E -A 4 0A 5 44,-2.2 -21,-0.2 -2,-0.5 4,-0.1 -0.636 20.6-127.7 -90.8 149.9 146.8 146.0 -9.4 26 26 A S S S+ 0 0 70 -23,-2.0 2,-0.3 -2,-0.2 -1,-0.1 0.629 93.1 15.9 -69.9 -15.1 150.5 146.5 -8.9 27 27 A Q S S- 0 0 75 -24,-0.3 2,-0.2 41,-0.1 -2,-0.1 -0.953 101.7 -71.3-150.4 167.4 150.7 148.3 -12.3 28 28 A S + 0 0 78 -2,-0.3 41,-0.2 1,-0.1 3,-0.1 -0.435 45.1 168.5 -66.3 129.8 148.3 149.8 -14.8 29 29 A I > - 0 0 1 39,-2.8 3,-2.4 1,-0.4 62,-0.2 0.035 36.0-131.6-133.2 30.7 146.2 147.3 -16.7 30 30 A G T 3 S- 0 0 37 1,-0.3 38,-3.1 2,-0.1 -1,-0.4 -0.402 71.2 -41.7 59.1-122.7 143.5 149.3 -18.5 31 31 A T T 3 S+ 0 0 62 36,-0.2 2,-1.3 -2,-0.2 -1,-0.3 -0.107 98.1 128.8-126.4 26.9 140.2 147.6 -17.8 32 32 A D < + 0 0 6 -3,-2.4 59,-2.0 18,-0.2 60,-0.5 -0.236 38.5 113.3 -81.5 47.0 141.6 144.1 -18.2 33 33 A I E -E 90 0B 0 -2,-1.3 18,-3.5 57,-0.2 2,-0.3 -0.894 45.6-165.2-118.5 148.3 140.2 142.8 -14.9 34 34 A H E -E 89 0B 0 55,-2.4 55,-1.5 -2,-0.3 2,-0.5 -0.995 10.5-138.5-136.3 142.9 137.5 140.2 -14.4 35 35 A W E -EF 88 48B 0 13,-3.0 12,-3.8 -2,-0.3 13,-1.6 -0.877 15.7-171.5-110.6 132.5 135.4 139.2 -11.4 36 36 A Y E -EF 87 46B 0 51,-2.8 51,-1.9 -2,-0.5 2,-0.4 -0.883 10.9-149.6-118.6 147.5 134.4 135.7 -10.3 37 37 A Q E -EF 86 45B 8 8,-2.5 8,-1.7 -2,-0.3 2,-0.4 -0.946 12.3-175.4-114.9 133.9 132.0 134.5 -7.6 38 38 A Q E -E 85 0B 13 47,-2.5 47,-2.5 -2,-0.4 2,-0.1 -0.952 7.8-159.9-133.5 114.7 132.6 131.2 -5.8 39 39 A R > - 0 0 85 -2,-0.4 3,-2.7 4,-0.4 45,-0.1 -0.446 50.3 -60.6 -84.9 164.6 130.0 129.9 -3.3 40 40 A T T 3 S- 0 0 80 43,-0.3 -1,-0.2 1,-0.3 3,-0.1 -0.221 122.4 -10.9 -47.1 119.4 130.8 127.4 -0.6 41 41 A N T 3 S+ 0 0 165 1,-0.2 -1,-0.3 -3,-0.1 2,-0.2 0.605 111.6 111.9 60.0 18.0 132.0 124.2 -2.4 42 42 A G S < S- 0 0 29 -3,-2.7 -1,-0.2 1,-0.0 3,-0.1 -0.598 70.7-104.2-108.1 174.1 131.0 125.4 -5.8 43 43 A S - 0 0 42 -2,-0.2 -4,-0.4 1,-0.1 -6,-0.1 -0.635 48.6 -77.6 -98.3 161.4 133.3 126.4 -8.7 44 44 A P - 0 0 5 0, 0.0 2,-0.4 0, 0.0 277,-0.3 -0.163 42.8-158.5 -54.3 145.8 134.1 130.0 -9.8 45 45 A R E -F 37 0B 133 -8,-1.7 -8,-2.5 275,-0.1 2,-0.2 -0.991 21.1-118.4-132.2 125.8 131.5 131.8 -11.9 46 46 A L E +F 36 0B 10 273,-0.4 -10,-0.3 -2,-0.4 3,-0.1 -0.427 38.1 165.5 -62.4 127.3 132.3 134.8 -14.1 47 47 A L E + 0 0 3 -12,-3.8 8,-0.8 1,-0.3 2,-0.3 0.717 65.8 13.6-110.7 -39.8 130.3 137.8 -12.9 48 48 A I E -FG 35 54B 2 -13,-1.6 -13,-3.0 6,-0.2 -1,-0.3 -0.972 64.2-159.7-145.2 126.4 132.0 140.7 -14.7 49 49 A K E >> S+ G 0 53B 52 4,-2.0 4,-2.4 -2,-0.3 3,-0.5 -0.803 73.7 8.0-107.8 151.7 134.4 140.8 -17.6 50 50 A Y T 34 S- 0 0 39 -2,-0.3 2,-2.9 1,-0.3 -1,-0.2 0.931 123.0 -68.9 46.3 57.2 136.7 143.6 -18.6 51 51 A A T 34 S+ 0 0 1 -18,-3.5 -1,-0.3 -3,-0.3 21,-0.2 -0.103 128.3 20.0 64.2 -44.4 136.0 145.6 -15.4 52 52 A S T <4 S+ 0 0 74 -2,-2.9 2,-0.7 -3,-0.5 12,-0.3 0.439 82.4 118.0-138.9 4.9 132.4 146.6 -16.2 53 53 A E E < -G 49 0B 98 -4,-2.4 -4,-2.0 -3,-0.2 -2,-0.1 -0.666 57.6-132.2 -85.6 116.6 130.7 144.4 -18.8 54 54 A S E -G 48 0B 98 -2,-0.7 2,-0.3 -6,-0.2 -6,-0.2 -0.275 16.2-137.5 -61.1 145.1 127.7 142.5 -17.4 55 55 A I > - 0 0 38 -8,-0.8 3,-1.4 -9,-0.1 2,-0.2 -0.799 29.2 -98.3-103.4 148.3 127.6 138.8 -18.2 56 56 A S T 3 S+ 0 0 120 -2,-0.3 -1,-0.0 1,-0.2 0, 0.0 -0.469 107.9 27.3 -68.4 135.3 124.3 137.2 -19.3 57 57 A G T 3 S+ 0 0 70 1,-0.3 -1,-0.2 -2,-0.2 3,-0.0 0.464 90.8 120.1 95.2 1.6 122.5 135.5 -16.5 58 58 A I S < S- 0 0 28 -3,-1.4 -1,-0.3 -11,-0.1 4,-0.1 -0.866 73.9 -99.1-104.7 132.2 123.9 137.6 -13.7 59 59 A P > - 0 0 47 0, 0.0 3,-2.3 0, 0.0 -1,-0.1 -0.152 28.4-124.9 -47.9 132.6 121.6 139.7 -11.4 60 60 A S T 3 S+ 0 0 106 1,-0.3 16,-0.1 -6,-0.0 -6,-0.0 0.659 104.2 76.8 -55.3 -16.8 121.4 143.3 -12.5 61 61 A R T 3 S+ 0 0 37 14,-0.1 15,-2.5 16,-0.1 2,-0.4 0.571 79.7 86.4 -74.2 -6.1 122.5 144.3 -9.0 62 62 A F E < +C 75 0A 11 -3,-2.3 2,-0.3 13,-0.2 13,-0.2 -0.805 54.6 151.3 -96.1 132.6 126.1 143.3 -9.8 63 63 A S E -C 74 0A 48 11,-2.3 11,-3.6 -2,-0.4 2,-0.3 -0.988 26.8-148.1-155.3 162.0 128.4 145.8 -11.5 64 64 A G E +C 73 0A 11 -2,-0.3 2,-0.3 -12,-0.3 9,-0.2 -0.941 14.1 175.6-134.0 156.3 132.0 146.8 -11.8 65 65 A S E +C 72 0A 64 7,-2.7 7,-3.2 -2,-0.3 2,-0.2 -0.968 27.9 75.0-152.4 166.2 134.0 150.0 -12.2 66 66 A G E -C 71 0A 35 -2,-0.3 2,-0.3 5,-0.2 5,-0.2 -0.465 39.0-150.0 117.0 169.6 137.5 151.3 -12.4 67 67 A S E > +C 70 0A 79 3,-0.9 2,-1.1 -2,-0.2 3,-0.8 -0.915 64.9 24.3-172.2 151.9 140.5 151.4 -14.8 68 68 A G T 3 S- 0 0 15 -38,-3.1 -39,-2.8 -2,-0.3 -41,-0.1 -0.742 129.6 -23.7 91.2 -98.5 144.3 151.5 -14.4 69 69 A T T 3 S+ 0 0 48 -2,-1.1 -44,-2.2 -41,-0.2 2,-0.6 0.520 120.6 73.8-126.7 -14.1 145.0 150.1 -11.0 70 70 A D E < +BC 24 67A 68 -3,-0.8 -3,-0.9 -46,-0.2 2,-0.3 -0.918 56.1 174.2-111.9 117.6 141.9 150.5 -8.9 71 71 A F E -BC 23 66A 1 -48,-3.0 -48,-2.4 -2,-0.6 2,-0.4 -0.913 11.4-162.9-124.0 149.2 138.9 148.3 -9.5 72 72 A T E -BC 22 65A 41 -7,-3.2 -7,-2.7 -2,-0.3 2,-0.5 -0.992 10.2-163.7-134.8 135.4 135.6 147.9 -7.8 73 73 A L E -BC 21 64A 0 -52,-2.2 -52,-3.4 -2,-0.4 2,-0.3 -0.979 24.1-167.2-112.1 120.3 132.9 145.2 -7.9 74 74 A S E -BC 20 63A 5 -11,-3.6 -11,-2.3 -2,-0.5 2,-0.4 -0.868 16.0-163.5-115.4 147.6 129.6 146.5 -6.4 75 75 A I E -BC 19 62A 0 -56,-2.4 -56,-2.3 -2,-0.3 2,-0.5 -0.970 16.1-143.4-127.5 114.2 126.4 144.8 -5.3 76 76 A N S S- 0 0 77 -15,-2.5 -58,-0.2 -2,-0.4 -59,-0.1 -0.671 82.7 -7.0 -78.3 125.1 123.3 146.9 -4.9 77 77 A S S S- 0 0 54 -2,-0.5 -1,-0.2 -61,-0.3 -59,-0.2 0.974 87.7-137.6 51.3 84.9 121.3 145.6 -1.9 78 78 A V - 0 0 4 -61,-2.2 -62,-1.8 -3,-0.3 2,-0.3 -0.095 24.1-172.8 -62.8 166.5 123.2 142.4 -1.0 79 79 A E > - 0 0 84 -64,-0.2 3,-4.5 -63,-0.1 4,-0.4 -0.960 41.6 -88.5-165.0 148.6 121.3 139.2 -0.1 80 80 A S G > S+ 0 0 25 1,-0.3 3,-1.7 -2,-0.3 90,-0.2 0.675 120.7 61.6 -26.2 -44.1 121.9 135.7 1.2 81 81 A E G 3 S+ 0 0 74 1,-0.3 -1,-0.3 88,-0.2 87,-0.1 0.743 98.4 60.1 -63.9 -20.0 122.4 134.3 -2.3 82 82 A D G < S+ 0 0 0 -3,-4.5 2,-0.3 2,-0.1 -1,-0.3 0.570 73.3 119.7 -83.0 -11.1 125.4 136.7 -2.6 83 83 A I < + 0 0 12 -3,-1.7 -43,-0.3 -4,-0.4 2,-0.3 -0.402 49.8 81.7 -60.3 119.3 127.2 135.2 0.4 84 84 A A S S- 0 0 7 20,-0.5 20,-3.1 -2,-0.3 2,-0.4 -0.975 79.9 -57.4 176.1-164.5 130.6 133.9 -1.0 85 85 A N E -EH 38 103B 56 -47,-2.5 -47,-2.5 -2,-0.3 2,-0.5 -0.883 40.8-156.4-107.3 139.8 134.1 134.6 -2.0 86 86 A Y E -EH 37 102B 0 16,-2.1 16,-1.8 -2,-0.4 2,-0.3 -0.970 10.4-172.8-124.5 120.7 134.9 137.1 -4.8 87 87 A Y E -E 36 0B 6 -51,-1.9 -51,-2.8 -2,-0.5 2,-0.3 -0.850 12.6-149.0-113.0 144.8 138.1 137.0 -6.8 88 88 A a E -E 35 0B 0 -2,-0.3 11,-2.8 -53,-0.2 2,-0.3 -0.755 15.3-168.4-105.1 157.2 139.5 139.4 -9.4 89 89 A Q E -EI 34 98B 0 -55,-1.5 -55,-2.4 -2,-0.3 2,-0.4 -0.996 7.6-146.1-146.4 141.5 141.6 138.2 -12.3 90 90 A Q E +EI 33 97B 0 7,-2.3 7,-1.0 -2,-0.3 -57,-0.2 -0.903 20.0 175.6-118.6 142.7 143.8 140.1 -14.8 91 91 A S + 0 0 1 -59,-2.0 380,-0.2 -2,-0.4 -58,-0.1 0.109 58.5 99.0-124.7 18.6 144.5 139.4 -18.4 92 92 A N S S+ 0 0 8 -60,-0.5 2,-0.3 1,-0.2 379,-0.3 0.933 91.1 19.2 -71.6 -47.3 146.6 142.5 -19.2 93 93 A R S S- 0 0 65 2,-0.2 -91,-0.2 377,-0.1 376,-0.2 -0.957 98.5 -68.7-129.6 148.4 150.0 140.8 -18.9 94 94 A W S S+ 0 0 5 374,-2.0 373,-2.1 -2,-0.3 374,-0.2 -0.553 107.3 39.9 -77.6 135.3 151.3 137.2 -19.0 95 95 A P S S- 0 0 5 0, 0.0 -93,-0.5 0, 0.0 -2,-0.2 0.609 87.4-127.8 -87.6 164.4 150.7 135.2 -16.9 96 96 A F - 0 0 3 -2,-0.1 2,-0.3 -5,-0.1 -5,-0.2 -0.364 33.5-164.0 -63.9 150.1 147.1 135.8 -15.9 97 97 A T E -I 90 0B 23 -7,-1.0 -7,-2.3 162,-0.1 2,-0.3 -0.967 12.6-138.8-141.2 157.7 146.8 136.2 -12.1 98 98 A F E -I 89 0B 24 -2,-0.3 -94,-0.4 160,-0.3 -9,-0.2 -0.878 20.2-120.4-115.7 147.6 144.1 136.1 -9.4 99 99 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