==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=26-JAN-2011 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 02-JUL-10 3NT3 . COMPND 2 MOLECULE: LSSMKATE2 RED FLUORESCENT PROTEIN; . SOURCE 2 ORGANISM_SCIENTIFIC: ARTIFICIAL GENE; . AUTHOR V.N.MALASHKEVICH,K.PIATKEVICH,S.C.ALMO,V.VERKHUSHA . 893 8 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 33289.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 658 73.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 52 5.8 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 406 45.5 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 12 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 82 9.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 81 9.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 30 3.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 12 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 4 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 4 0 2 6 0 4 4 8 4 12 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 3 A E 0 0 169 0, 0.0 3,-0.1 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0-175.2 -71.6 -5.3 -16.6 2 4 A L + 0 0 79 1,-0.2 2,-0.9 75,-0.1 75,-0.0 0.831 360.0 55.5 -83.5 -36.9 -68.5 -3.8 -14.9 3 5 A I S S- 0 0 2 74,-0.1 -1,-0.2 77,-0.1 4,-0.1 -0.861 86.4-163.6 -98.7 102.5 -68.0 -1.2 -17.6 4 6 A K - 0 0 130 -2,-0.9 29,-0.2 2,-0.3 -2,-0.1 -0.212 35.8 -98.0 -81.6 171.0 -71.3 0.6 -17.9 5 7 A E S S+ 0 0 128 1,-0.1 2,-0.4 26,-0.1 28,-0.3 0.840 117.9 34.0 -56.3 -38.3 -72.7 2.8 -20.6 6 8 A N S S+ 0 0 87 25,-0.2 2,-0.4 26,-0.1 -2,-0.3 -0.980 76.8 178.1-116.5 135.0 -71.7 5.8 -18.4 7 9 A M E -A 30 0A 4 23,-2.1 23,-2.2 -2,-0.4 2,-0.3 -0.998 15.5-149.3-136.5 142.7 -68.5 5.7 -16.3 8 10 A H E -A 29 0A 109 101,-0.5 103,-2.4 -2,-0.4 2,-0.3 -0.687 15.1-161.7-100.1 157.7 -66.8 8.2 -14.1 9 11 A M E -Ab 28 111A 2 19,-2.1 19,-2.1 -2,-0.3 2,-0.4 -0.976 12.5-163.2-138.3 155.1 -63.1 8.4 -13.4 10 12 A K E -Ab 27 112A 103 101,-2.2 103,-2.8 -2,-0.3 2,-0.4 -0.990 16.2-165.2-132.0 140.4 -60.7 10.0 -10.9 11 13 A L E -Ab 26 113A 1 15,-2.5 15,-2.0 -2,-0.4 2,-0.4 -0.963 13.8-166.3-133.0 145.1 -57.1 10.4 -11.6 12 14 A Y E -Ab 25 114A 107 101,-2.5 103,-3.0 -2,-0.4 2,-0.4 -0.999 16.1-177.8-127.2 125.9 -53.9 11.2 -9.5 13 15 A M E +Ab 24 115A 4 11,-2.8 11,-2.4 -2,-0.4 2,-0.3 -0.982 8.0 173.7-128.6 136.5 -50.8 12.2 -11.3 14 16 A E E +Ab 23 116A 42 101,-2.0 103,-2.1 -2,-0.4 2,-0.3 -0.991 20.9 122.2-133.5 146.6 -47.3 13.0 -10.0 15 17 A G E -Ab 22 117A 3 7,-2.3 7,-2.4 -2,-0.3 2,-0.3 -0.994 44.1-113.6-176.9-172.4 -44.1 13.7 -11.9 16 18 A T E -Ab 21 118A 6 101,-1.5 103,-3.3 -2,-0.3 2,-0.4 -0.992 13.5-170.0-139.9 138.1 -41.2 15.8 -12.8 17 19 A V E > S-Ab 20 119A 0 3,-1.6 3,-1.6 -2,-0.3 103,-0.2 -0.989 86.1 -15.5-130.3 118.8 -40.2 17.5 -16.2 18 20 A N T 3 S- 0 0 24 101,-2.3 102,-0.1 -2,-0.4 3,-0.1 0.883 130.9 -54.3 48.4 45.1 -36.7 19.0 -16.3 19 21 A N T 3 S+ 0 0 66 100,-0.5 2,-0.7 1,-0.2 -1,-0.3 0.647 106.9 130.0 64.5 20.2 -36.6 18.6 -12.5 20 22 A H E < -A 17 0A 55 -3,-1.6 -3,-1.6 2,-0.0 -1,-0.2 -0.916 48.9-147.8-103.3 113.2 -39.8 20.6 -12.0 21 23 A H E +A 16 0A 59 -2,-0.7 2,-0.3 -5,-0.2 -5,-0.2 -0.494 25.7 159.5 -80.4 144.2 -42.0 18.6 -9.7 22 24 A F E -A 15 0A 0 -7,-2.4 -7,-2.3 -2,-0.2 2,-0.3 -0.985 25.8-151.1-153.2 162.9 -45.8 18.7 -9.9 23 25 A K E -AC 14 45A 91 22,-2.1 21,-2.2 -2,-0.3 22,-1.6 -0.994 14.5-173.4-133.5 144.0 -49.0 16.9 -9.0 24 26 A C E -AC 13 43A 2 -11,-2.4 -11,-2.8 -2,-0.3 2,-0.3 -0.941 5.5-166.2-127.5 156.9 -52.4 16.9 -10.8 25 27 A T E -AC 12 42A 46 17,-2.4 17,-3.1 -2,-0.3 2,-0.3 -0.959 10.6-178.6-131.0 154.7 -55.8 15.5 -10.0 26 28 A S E -AC 11 41A 8 -15,-2.0 -15,-2.5 -2,-0.3 2,-0.3 -0.991 13.5-166.8-146.9 161.1 -58.7 15.1 -12.3 27 29 A E E +AC 10 40A 87 13,-1.8 13,-3.0 -2,-0.3 2,-0.3 -0.982 27.0 130.9-137.9 151.0 -62.3 13.9 -12.4 28 30 A G E -AC 9 39A 1 -19,-2.1 -19,-2.1 -2,-0.3 2,-0.3 -0.939 33.9-129.7-171.2-164.6 -64.5 13.2 -15.3 29 31 A E E +AC 8 38A 63 9,-1.5 9,-2.5 -2,-0.3 2,-0.3 -0.970 21.2 166.7-159.5 164.2 -66.9 10.9 -17.1 30 32 A G E -AC 7 37A 8 -23,-2.2 -23,-2.1 -2,-0.3 7,-0.3 -0.959 41.5 -96.8-167.8-177.9 -67.5 9.3 -20.5 31 33 A K E >>> - C 0 36A 49 5,-2.2 5,-1.4 -2,-0.3 3,-1.2 -0.913 29.7-164.8-115.6 98.5 -69.3 6.8 -22.6 32 34 A P T 345S+ 0 0 0 0, 0.0 34,-0.3 0, 0.0 -1,-0.1 0.781 87.2 47.4 -61.8 -28.3 -66.9 3.8 -22.9 33 35 A Y T 345S+ 0 0 106 -28,-0.3 -28,-0.1 1,-0.2 32,-0.1 0.585 113.8 47.0 -91.5 -11.2 -68.8 2.2 -25.9 34 36 A E T <45S- 0 0 109 -3,-1.2 33,-0.3 -29,-0.2 -1,-0.2 0.519 108.1-126.3 -95.7 -14.2 -69.0 5.5 -27.8 35 37 A G T <5 + 0 0 0 -4,-1.2 178,-2.3 1,-0.2 2,-0.3 0.749 65.0 128.4 75.1 27.1 -65.3 6.2 -27.2 36 38 A T E < +CD 31 212A 30 -5,-1.4 -5,-2.2 176,-0.2 2,-0.3 -0.860 26.0 159.7-118.9 150.5 -65.9 9.7 -25.7 37 39 A Q E -CD 30 211A 10 174,-1.8 174,-2.4 -2,-0.3 2,-0.4 -0.984 22.3-152.8-165.4 151.2 -64.8 11.3 -22.4 38 40 A T E -CD 29 210A 26 -9,-2.5 -9,-1.5 -2,-0.3 2,-0.4 -0.987 9.9-167.7-127.4 139.2 -64.2 14.5 -20.6 39 41 A M E -CD 28 209A 6 170,-2.7 170,-2.5 -2,-0.4 2,-0.6 -0.997 12.5-154.5-126.9 137.1 -61.7 15.3 -17.8 40 42 A R E -CD 27 208A 56 -13,-3.0 -13,-1.8 -2,-0.4 2,-0.4 -0.953 27.6-163.9-105.5 116.3 -61.6 18.4 -15.6 41 43 A I E -CD 26 207A 0 166,-3.5 166,-2.4 -2,-0.6 2,-0.4 -0.859 14.1-168.7-115.7 136.0 -58.0 18.7 -14.4 42 44 A K E -CD 25 206A 122 -17,-3.1 -17,-2.4 -2,-0.4 2,-0.7 -0.971 21.4-138.5-119.0 135.7 -56.3 20.6 -11.6 43 45 A V E +C 24 0A 19 162,-2.4 -19,-0.3 -2,-0.4 3,-0.1 -0.846 29.2 175.8 -84.1 114.6 -52.6 21.3 -11.0 44 46 A V E + 0 0 83 -21,-2.2 2,-0.3 -2,-0.7 -20,-0.2 0.567 60.0 23.6-104.8 -12.5 -52.5 20.8 -7.3 45 47 A E E S+C 23 0A 87 -22,-1.6 -22,-2.1 1,-0.0 -1,-0.3 -0.971 118.9 21.6-149.4 143.4 -48.8 21.1 -6.5 46 48 A G S S- 0 0 19 -2,-0.3 -2,-0.1 1,-0.3 -22,-0.0 0.568 89.8-173.5 78.1 11.0 -46.0 22.8 -8.4 47 49 A G + 0 0 42 2,-0.1 -1,-0.3 -24,-0.1 2,-0.1 -0.528 56.7 50.4 -82.3 154.5 -48.3 25.2 -10.4 48 50 A P S S- 0 0 58 0, 0.0 156,-0.1 0, 0.0 155,-0.1 0.586 88.6-136.8 -71.2 142.8 -48.0 27.1 -12.5 49 51 A L - 0 0 3 154,-0.5 2,-0.4 1,-0.1 4,-0.1 -0.494 2.4-140.6 -67.7 132.6 -46.1 24.5 -14.6 50 52 A P S S+ 0 0 56 0, 0.0 2,-0.3 0, 0.0 -1,-0.1 -0.095 80.6 53.4 -86.5 37.4 -43.1 26.1 -16.3 51 53 A F S S- 0 0 0 -2,-0.4 2,-0.3 79,-0.1 78,-0.2 -0.971 103.1 -66.2-160.8 163.3 -43.8 24.0 -19.5 52 54 A A > - 0 0 0 76,-2.1 3,-1.8 -2,-0.3 4,-0.2 -0.400 31.8-148.5 -60.8 119.6 -46.5 23.2 -22.0 53 55 A F T >> S+ 0 0 1 1,-0.3 3,-2.8 -2,-0.3 4,-0.7 0.818 90.8 80.2 -55.1 -30.7 -49.4 21.3 -20.3 54 56 A D H >> S+ 0 0 6 1,-0.3 3,-0.8 2,-0.2 4,-0.5 0.777 80.3 64.2 -49.1 -31.5 -49.9 19.7 -23.8 55 57 A I H <4 S+ 0 0 2 -3,-1.8 -1,-0.3 1,-0.3 -2,-0.2 0.680 107.1 42.9 -72.6 -13.2 -47.0 17.3 -23.0 56 58 A L H X4 S+ 0 0 0 -3,-2.8 3,-2.1 -4,-0.2 4,-0.4 0.524 90.4 90.7 -96.0 -12.6 -49.1 15.8 -20.2 57 59 A A H X< S+ 0 0 2 -3,-0.8 3,-1.2 -4,-0.7 -2,-0.1 0.851 83.1 52.0 -62.0 -37.7 -52.4 15.7 -22.1 58 60 A T T 3< S+ 0 0 41 -4,-0.5 -1,-0.3 1,-0.2 -2,-0.1 0.360 101.0 64.0 -81.6 6.4 -52.0 12.2 -23.5 59 61 A S T < 0 0 4 -3,-2.1 -1,-0.2 1,-0.1 -2,-0.2 0.455 360.0 360.0-102.7 -6.1 -51.3 10.8 -20.0 60 62 A F < 0 0 11 -3,-1.2 -2,-0.2 -4,-0.4 -1,-0.1 0.618 360.0 360.0 -85.8 360.0 -54.8 11.7 -18.6 61 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 62 66 A S > 0 0 15 0, 0.0 3,-1.7 0, 0.0 -2,-0.0 0.000 360.0 360.0 360.0 62.3 -57.8 6.4 -20.6 63 67 A Y T 3 + 0 0 7 1,-0.3 3,-0.4 2,-0.1 14,-0.2 0.561 360.0 75.7 -70.4 -16.7 -58.1 3.3 -22.8 64 68 A T T 3 S+ 0 0 0 1,-0.2 2,-0.9 12,-0.1 -1,-0.3 0.803 89.3 64.4 -62.6 -26.7 -61.4 2.0 -21.3 65 69 A F S < S+ 0 0 0 -3,-1.7 2,-0.4 -30,-0.1 -1,-0.2 -0.451 74.0 115.7 -98.8 55.8 -63.1 4.8 -23.3 66 70 A I - 0 0 0 -2,-0.9 2,-1.1 -3,-0.4 -31,-0.2 -0.997 67.5-128.9-119.8 128.5 -62.3 3.5 -26.8 67 71 A N E -e 214 0A 49 146,-2.4 148,-2.7 -2,-0.4 2,-1.3 -0.645 21.7-158.0 -73.4 100.1 -65.1 2.4 -29.1 68 72 A H E > -e 215 0A 22 -2,-1.1 3,-0.5 146,-0.2 148,-0.1 -0.728 21.0-161.8 -84.4 96.6 -63.8 -1.0 -30.1 69 73 A T G > + 0 0 35 146,-1.4 3,-2.0 -2,-1.3 145,-0.0 -0.263 57.4 22.8 -79.4 163.5 -65.8 -1.4 -33.4 70 74 A Q G 3 S- 0 0 138 1,-0.3 -1,-0.2 146,-0.1 146,-0.0 0.715 127.3 -65.5 58.1 27.6 -66.6 -4.5 -35.4 71 75 A G G < S+ 0 0 65 -3,-0.5 -1,-0.3 1,-0.2 -2,-0.1 0.635 75.7 172.1 69.7 16.5 -66.1 -6.8 -32.5 72 76 A I < - 0 0 5 -3,-2.0 -1,-0.2 143,-0.1 -4,-0.1 -0.387 47.8-103.6 -51.7 124.2 -62.4 -6.2 -32.1 73 77 A P - 0 0 20 0, 0.0 2,-1.0 0, 0.0 3,-0.1 -0.353 34.7-136.4 -55.0 131.7 -61.5 -8.0 -28.8 74 78 A D > - 0 0 20 1,-0.2 4,-2.5 111,-0.1 5,-0.2 -0.797 21.2-174.2-105.1 95.4 -61.2 -5.2 -26.2 75 79 A F H > S+ 0 0 4 -2,-1.0 4,-0.6 1,-0.2 -1,-0.2 0.826 84.3 48.3 -55.3 -37.5 -58.1 -5.9 -24.1 76 80 A F H >4 S+ 0 0 0 2,-0.2 3,-1.1 -13,-0.2 4,-0.4 0.973 113.4 41.3 -74.8 -54.1 -58.9 -3.0 -21.8 77 81 A K H >4 S+ 0 0 13 1,-0.3 3,-1.1 -14,-0.2 -2,-0.2 0.875 112.8 56.7 -65.3 -33.7 -62.6 -3.6 -21.1 78 82 A Q H 3< S+ 0 0 50 -4,-2.5 -1,-0.3 1,-0.2 -2,-0.2 0.695 92.4 72.5 -68.0 -19.1 -62.0 -7.3 -20.7 79 83 A S T XX S+ 0 0 0 -3,-1.1 4,-2.0 -4,-0.6 3,-0.6 0.639 80.8 139.5 -74.4 -17.8 -59.4 -6.7 -18.0 80 84 A F T <4 + 0 0 9 -3,-1.1 -78,-0.1 -4,-0.4 -77,-0.1 -0.469 51.4 27.7 -74.6 149.0 -61.9 -5.7 -15.4 81 85 A P T 34 S+ 0 0 75 0, 0.0 25,-0.5 0, 0.0 -1,-0.3 -0.982 131.6 41.6 -80.5 -13.5 -62.4 -6.2 -12.5 82 86 A E T <4 S- 0 0 71 -3,-0.6 -2,-0.3 1,-0.2 2,-0.2 0.866 96.6-157.1 -65.9 -38.5 -58.6 -6.6 -12.1 83 87 A G < - 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