==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=9-DEC-2009 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER COMPLEX (MHC I/PEPTIDE) 18-JUN-98 1OSZ . COMPND 2 MOLECULE: MHC CLASS I H-2KB HEAVY CHAIN; . SOURCE 2 ORGANISM_SCIENTIFIC: MUS MUSCULUS; . AUTHOR Y.GHENDLER,M.-K.TENG,J.-H.LIU,T.WITTE,J.LIU,K.S.KIM,P.KERN, . 381 3 3 3 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 18681.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 250 65.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 134 35.2 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 1 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 4 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 4 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 23 6.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 32 8.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 57 15.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 4 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 1 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 2 5 3 1 2 2 2 1 3 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 3 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 A G 0 0 67 0, 0.0 2,-0.2 0, 0.0 102,-0.0 0.000 360.0 360.0 360.0-163.3 66.6 45.5 3.6 2 2 A P - 0 0 86 0, 0.0 2,-0.3 0, 0.0 102,-0.2 -0.509 360.0-162.9 -83.9 153.3 63.9 47.2 1.4 3 3 A H E -A 103 0A 32 100,-2.4 100,-2.5 101,-0.2 2,-0.3 -0.915 1.3-156.6-133.3 159.8 60.4 48.1 2.7 4 4 A S E -A 102 0A 19 -2,-0.3 2,-0.5 98,-0.2 98,-0.2 -0.976 15.0-150.9-142.8 155.8 57.1 49.0 1.1 5 5 A L E +A 101 0A 0 96,-2.2 96,-1.5 -2,-0.3 2,-0.3 -0.993 28.3 178.7-123.3 118.6 53.9 50.8 1.7 6 6 A R E -AB 100 27A 57 21,-2.9 21,-3.1 -2,-0.5 2,-0.4 -0.952 17.9-162.1-128.6 147.1 50.9 49.4 -0.2 7 7 A Y E -AB 99 26A 0 92,-2.1 92,-1.5 -2,-0.3 2,-0.7 -0.989 4.4-162.9-128.9 122.4 47.2 50.2 -0.4 8 8 A F E -AB 98 25A 4 17,-3.4 17,-1.6 -2,-0.4 2,-0.4 -0.913 14.4-176.7-106.4 112.0 44.7 47.7 -1.8 9 9 A V E -AB 97 24A 0 88,-2.6 88,-2.5 -2,-0.7 2,-0.4 -0.903 4.0-177.1-113.8 140.3 41.4 49.4 -2.7 10 10 A T E -AB 96 23A 9 13,-2.1 13,-2.5 -2,-0.4 2,-0.5 -0.996 9.0-175.7-137.6 132.7 38.3 47.6 -4.0 11 11 A A E +AB 95 22A 2 84,-2.4 84,-2.6 -2,-0.4 2,-0.4 -0.996 11.1 176.2-126.9 124.5 35.0 49.0 -5.2 12 12 A V E -AB 94 21A 26 9,-2.0 9,-2.6 -2,-0.5 82,-0.2 -0.984 19.0-141.5-135.4 125.2 32.2 46.5 -6.1 13 13 A S - 0 0 19 80,-1.9 6,-0.1 -2,-0.4 80,-0.0 -0.452 9.9-169.0 -82.3 156.0 28.7 47.4 -7.1 14 14 A R > - 0 0 72 4,-0.5 3,-2.6 -2,-0.1 78,-0.1 -0.603 19.1-147.6-144.4 74.5 25.6 45.4 -6.0 15 15 A P T 3 S+ 0 0 52 0, 0.0 77,-0.1 0, 0.0 4,-0.1 -0.122 83.7 19.1 -45.8 134.9 22.7 46.6 -8.0 16 16 A G T 3 S+ 0 0 84 2,-0.1 3,-0.1 1,-0.0 74,-0.0 0.324 112.8 76.5 84.9 -9.3 19.4 46.4 -6.1 17 17 A L S < S- 0 0 138 -3,-2.6 2,-0.2 1,-0.4 -1,-0.0 0.036 103.4-102.2-121.1 25.2 21.2 46.3 -2.7 18 18 A G S S- 0 0 59 -4,-0.0 -4,-0.5 3,-0.0 -1,-0.4 -0.550 72.2 -2.0 95.0-158.9 22.2 49.9 -2.3 19 19 A E S S- 0 0 104 -2,-0.2 3,-0.0 1,-0.1 2,-0.0 -0.198 83.5 -89.0 -68.1 161.4 25.5 51.6 -2.9 20 20 A P - 0 0 15 0, 0.0 2,-0.4 0, 0.0 -7,-0.3 -0.343 43.3-106.6 -69.9 154.2 28.7 49.7 -3.9 21 21 A R E -B 12 0A 84 -9,-2.6 -9,-2.0 -3,-0.1 2,-0.5 -0.692 37.0-161.9 -82.6 131.5 30.9 48.3 -1.1 22 22 A Y E +B 11 0A 3 -2,-0.4 16,-2.9 -11,-0.2 2,-0.3 -0.964 11.6 176.4-120.8 128.5 34.1 50.3 -0.8 23 23 A M E -BC 10 37A 27 -13,-2.5 -13,-2.1 -2,-0.5 2,-0.4 -0.967 14.4-173.1-133.7 148.4 37.3 49.1 0.9 24 24 A E E -BC 9 36A 1 12,-2.5 12,-2.7 -2,-0.3 2,-0.4 -0.998 6.6-179.7-137.3 129.2 40.8 50.3 1.6 25 25 A V E -BC 8 35A 1 -17,-1.6 -17,-3.4 -2,-0.4 2,-0.4 -0.980 8.8-161.5-135.0 122.6 43.6 48.2 3.1 26 26 A G E -BC 7 34A 0 8,-2.0 7,-2.3 -2,-0.4 8,-1.1 -0.821 4.3-169.6-107.0 144.9 47.1 49.4 3.8 27 27 A Y E -BC 6 32A 8 -21,-3.1 -21,-2.9 -2,-0.4 5,-0.2 -0.987 19.4-161.0-131.8 140.0 50.3 47.4 4.4 28 28 A V S S- 0 0 0 3,-1.8 2,-0.5 -2,-0.4 -23,-0.2 -0.394 86.0 -42.6-114.8 51.8 53.7 48.5 5.6 29 29 A D S S- 0 0 23 -26,-0.1 -23,-0.1 1,-0.1 -26,-0.0 -0.999 121.6 -24.9 126.7-119.3 55.4 45.3 4.3 30 30 A D S S+ 0 0 62 -2,-0.5 2,-0.5 149,-0.1 -1,-0.1 0.254 116.9 94.6-108.1 11.6 53.4 42.1 5.0 31 31 A T S S- 0 0 18 148,-0.0 -3,-1.8 18,-0.0 2,-0.2 -0.910 72.9-132.1-111.4 122.3 51.6 43.6 8.0 32 32 A E E +C 27 0A 19 -2,-0.5 -5,-0.2 -5,-0.2 3,-0.1 -0.467 33.6 165.1 -65.7 133.1 48.2 45.2 7.7 33 33 A F E + 0 0 0 -7,-2.3 15,-2.1 1,-0.4 16,-0.9 0.548 56.9 15.9-128.5 -13.5 48.2 48.5 9.5 34 34 A V E +CD 26 47A 0 -8,-1.1 -8,-2.0 13,-0.3 -1,-0.4 -0.979 58.9 176.0-155.6 160.2 45.1 50.5 8.5 35 35 A R E -CD 25 46A 60 11,-1.2 11,-1.9 -2,-0.3 2,-0.4 -0.972 15.8-157.7-168.4 153.5 41.8 49.8 6.8 36 36 A F E -C 24 0A 0 -12,-2.7 -12,-2.5 -2,-0.3 2,-0.4 -0.998 10.4-178.2-138.4 130.6 38.5 51.3 5.7 37 37 A D E > -C 23 0A 23 7,-0.4 3,-2.1 -2,-0.4 7,-0.2 -0.955 20.2-156.3-135.6 115.5 35.2 49.5 5.0 38 38 A S T 3 S+ 0 0 19 -16,-2.9 -15,-0.1 -2,-0.4 -1,-0.1 0.646 95.5 63.2 -61.9 -15.7 32.0 51.2 3.8 39 39 A D T 3 S+ 0 0 69 -17,-0.3 -1,-0.3 3,-0.1 -16,-0.1 0.528 77.6 112.2 -87.9 -6.5 30.1 48.3 5.3 40 40 A A S < S- 0 0 30 -3,-2.1 -4,-0.0 1,-0.1 0, 0.0 -0.249 84.7-110.2 -63.3 157.1 31.2 49.0 8.8 41 41 A E S S+ 0 0 191 1,-0.3 -1,-0.1 2,-0.0 -3,-0.0 0.828 116.2 14.7 -56.7 -34.7 28.5 50.2 11.3 42 42 A N S S- 0 0 110 -5,-0.1 -1,-0.3 2,-0.1 -3,-0.1 -0.692 94.7-135.6-144.2 80.9 30.2 53.6 11.2 43 43 A P + 0 0 31 0, 0.0 2,-0.3 0, 0.0 -5,-0.1 -0.108 46.4 137.1 -43.2 117.1 32.7 53.7 8.2 44 44 A R - 0 0 101 -7,-0.2 -7,-0.4 -9,-0.1 2,-0.4 -0.987 58.9 -92.9-161.2 157.7 36.0 55.2 9.3 45 45 A Y - 0 0 4 15,-0.5 -9,-0.2 -2,-0.3 19,-0.1 -0.626 50.6-179.2 -77.3 131.6 39.7 54.7 9.0 46 46 A E E -D 35 0A 72 -11,-1.9 -11,-1.2 -2,-0.4 2,-0.0 -0.964 29.6-101.8-135.5 152.9 41.0 52.6 11.8 47 47 A P E +D 34 0A 60 0, 0.0 -13,-0.3 0, 0.0 3,-0.1 -0.331 26.3 178.7 -68.3 149.8 44.3 51.2 13.0 48 48 A R + 0 0 76 -15,-2.1 2,-0.4 1,-0.2 -14,-0.2 0.338 69.6 57.2-130.4 -3.0 45.1 47.6 12.4 49 49 A A S > S- 0 0 16 -16,-0.9 3,-1.6 1,-0.1 -1,-0.2 -0.980 82.6-127.3-129.9 140.5 48.6 47.5 13.9 50 50 A R G > S+ 0 0 207 -2,-0.4 3,-1.6 1,-0.3 4,-0.1 0.813 105.3 61.3 -59.3 -33.9 49.3 48.5 17.5 51 51 A W G > S+ 0 0 11 1,-0.3 3,-1.4 2,-0.1 -1,-0.3 0.625 85.8 77.3 -70.5 -9.7 52.0 51.0 16.7 52 52 A M G X S+ 0 0 1 -3,-1.6 3,-1.5 1,-0.3 -1,-0.3 0.553 72.3 82.3 -75.4 -4.5 49.5 53.0 14.7 53 53 A E G < S+ 0 0 128 -3,-1.6 -1,-0.3 1,-0.3 -2,-0.1 0.610 75.6 74.9 -72.6 -10.2 48.2 54.2 18.1 54 54 A Q G < S+ 0 0 98 -3,-1.4 2,-0.4 -4,-0.1 -1,-0.3 0.537 76.6 93.1 -78.8 -7.4 51.1 56.7 17.9 55 55 A E S < S- 0 0 16 -3,-1.5 5,-0.1 1,-0.1 -3,-0.0 -0.719 79.6-117.3 -90.8 137.5 49.2 58.8 15.3 56 56 A G >> - 0 0 35 -2,-0.4 3,-1.1 1,-0.1 4,-1.0 -0.089 28.9-100.2 -66.5 167.9 47.0 61.7 16.5 57 57 A P H 3> S+ 0 0 110 0, 0.0 4,-1.7 0, 0.0 5,-0.1 0.822 121.1 64.2 -58.3 -31.3 43.2 62.0 16.2 58 58 A E H 3> S+ 0 0 130 1,-0.2 4,-2.9 2,-0.2 5,-0.2 0.850 94.4 60.1 -61.8 -34.7 43.8 64.3 13.2 59 59 A Y H <> S+ 0 0 10 -3,-1.1 4,-2.8 1,-0.2 5,-0.3 0.966 108.1 42.7 -57.4 -54.2 45.4 61.5 11.3 60 60 A W H X S+ 0 0 27 -4,-1.0 4,-1.5 1,-0.2 -15,-0.5 0.796 113.4 53.6 -63.2 -29.9 42.2 59.4 11.5 61 61 A E H X S+ 0 0 97 -4,-1.7 4,-2.2 2,-0.2 -1,-0.2 0.937 112.8 42.3 -70.4 -47.7 40.1 62.4 10.7 62 62 A R H X S+ 0 0 130 -4,-2.9 4,-2.4 2,-0.2 -2,-0.2 0.921 115.7 47.8 -65.4 -46.6 42.0 63.2 7.6 63 63 A E H X S+ 0 0 0 -4,-2.8 4,-2.3 -5,-0.2 -1,-0.2 0.838 112.7 50.3 -64.4 -33.5 42.3 59.6 6.3 64 64 A T H X S+ 0 0 3 -4,-1.5 4,-2.3 -5,-0.3 -1,-0.2 0.921 110.6 48.5 -70.4 -44.5 38.6 59.1 7.0 65 65 A Q H X S+ 0 0 98 -4,-2.2 4,-1.6 1,-0.2 -2,-0.2 0.926 114.4 46.1 -61.8 -44.5 37.6 62.2 5.0 66 66 A K H X S+ 0 0 29 -4,-2.4 4,-2.6 1,-0.2 -2,-0.2 0.925 110.3 52.8 -64.9 -44.0 39.9 61.2 2.1 67 67 A A H X S+ 0 0 0 -4,-2.3 4,-2.2 1,-0.2 -1,-0.2 0.895 108.9 51.1 -58.0 -40.5 38.6 57.6 2.1 68 68 A K H X S+ 0 0 82 -4,-2.3 4,-1.8 2,-0.2 -1,-0.2 0.839 108.8 50.1 -66.9 -33.8 35.0 58.9 2.0 69 69 A G H X S+ 0 0 38 -4,-1.6 4,-2.6 2,-0.2 -1,-0.2 0.916 110.2 51.8 -69.4 -41.1 35.9 61.1 -1.0 70 70 A N H X S+ 0 0 1 -4,-2.6 4,-2.7 1,-0.2 -2,-0.2 0.880 105.8 55.0 -60.0 -41.5 37.5 58.1 -2.6 71 71 A E H X S+ 0 0 21 -4,-2.2 4,-1.8 2,-0.2 -1,-0.2 0.949 110.4 43.5 -58.8 -52.2 34.3 56.1 -2.1 72 72 A Q H X S+ 0 0 90 -4,-1.8 4,-2.0 1,-0.2 3,-0.3 0.943 113.1 54.1 -59.3 -46.2 32.1 58.6 -3.8 73 73 A S H X S+ 0 0 29 -4,-2.6 4,-1.7 1,-0.2 310,-0.2 0.894 111.1 44.0 -55.2 -43.2 34.7 58.9 -6.5 74 74 A F H X S+ 0 0 1 -4,-2.7 4,-2.1 2,-0.2 -1,-0.2 0.757 106.3 60.7 -75.4 -23.7 34.7 55.1 -7.1 75 75 A R H X S+ 0 0 59 -4,-1.8 4,-1.1 -3,-0.3 -1,-0.2 0.914 109.4 43.4 -66.7 -41.9 30.9 54.9 -7.0 76 76 A V H X S+ 0 0 87 -4,-2.0 4,-2.8 1,-0.2 -2,-0.2 0.884 108.0 61.0 -68.2 -39.4 31.0 57.3 -10.0 77 77 A D H X S+ 0 0 2 -4,-1.7 4,-2.7 1,-0.2 5,-0.2 0.906 101.7 51.1 -53.3 -48.6 33.8 55.2 -11.6 78 78 A L H X S+ 0 0 9 -4,-2.1 4,-1.1 1,-0.2 -1,-0.2 0.870 111.2 48.4 -59.6 -38.2 31.6 52.1 -11.7 79 79 A R H >X S+ 0 0 161 -4,-1.1 4,-1.2 2,-0.2 3,-0.5 0.943 111.2 51.0 -66.4 -47.6 28.9 54.1 -13.5 80 80 A T H >X S+ 0 0 27 -4,-2.8 4,-2.2 1,-0.2 3,-0.7 0.902 107.5 51.7 -55.8 -47.7 31.4 55.6 -15.9 81 81 A L H 3X S+ 0 0 1 -4,-2.7 4,-2.8 1,-0.3 -1,-0.2 0.772 100.6 62.9 -63.6 -26.3 32.8 52.1 -16.8 82 82 A L H <<>S+ 0 0 24 -4,-1.1 5,-3.2 -3,-0.5 -1,-0.3 0.887 109.6 41.7 -64.7 -37.1 29.3 50.9 -17.5 83 83 A G H X<5S+ 0 0 56 -4,-1.2 3,-0.5 -3,-0.7 -2,-0.2 0.897 112.1 52.4 -76.4 -42.3 29.2 53.5 -20.3 84 84 A Y H 3<5S+ 0 0 52 -4,-2.2 -2,-0.2 1,-0.3 -3,-0.2 0.942 122.4 32.7 -58.3 -46.9 32.8 52.9 -21.5 85 85 A Y T 3<5S- 0 0 68 -4,-2.8 -1,-0.3 -5,-0.1 -2,-0.2 0.402 106.3-126.6 -90.5 2.2 32.0 49.2 -21.8 86 86 A N T < 5 + 0 0 149 -3,-0.5 2,-0.2 -4,-0.2 -3,-0.2 0.954 53.5 162.5 50.9 55.2 28.3 49.7 -22.7 87 87 A Q < - 0 0 64 -5,-3.2 -1,-0.2 -6,-0.1 2,-0.1 -0.686 41.0 -96.4-106.3 160.8 27.3 47.3 -19.9 88 88 A S > - 0 0 85 -2,-0.2 3,-0.9 1,-0.1 -1,-0.1 -0.401 21.7-130.1 -75.0 149.9 23.9 46.8 -18.2 89 89 A K T 3 S+ 0 0 156 1,-0.2 -1,-0.1 -2,-0.1 -7,-0.0 0.379 99.2 68.3 -79.4 3.1 23.0 48.5 -14.9 90 90 A G T 3 S+ 0 0 56 -75,-0.1 2,-0.3 2,-0.1 -1,-0.2 0.566 80.9 83.2-101.8 -8.0 21.9 45.3 -13.4 91 91 A G S < S- 0 0 30 -3,-0.9 2,-0.3 -77,-0.1 -78,-0.1 -0.687 77.2-117.1 -98.9 151.7 25.2 43.4 -13.1 92 92 A S - 0 0 70 -2,-0.3 2,-0.3 -78,-0.1 -78,-0.1 -0.648 35.6-175.8 -84.5 138.4 27.8 43.6 -10.4 93 93 A H - 0 0 24 -2,-0.3 -80,-1.9 -80,-0.0 2,-0.4 -0.929 12.6-139.7-133.9 158.4 31.2 44.8 -11.4 94 94 A T E -A 12 0A 3 -2,-0.3 25,-3.3 -82,-0.2 2,-0.4 -0.957 9.2-169.0-127.8 139.7 34.6 45.3 -9.7 95 95 A I E -AE 11 118A 1 -84,-2.6 -84,-2.4 -2,-0.4 2,-0.4 -0.983 14.0-172.2-121.8 130.8 37.3 47.9 -9.7 96 96 A Q E -AE 10 117A 0 21,-2.3 21,-1.6 -2,-0.4 2,-0.4 -0.977 3.8-176.3-127.5 139.1 40.7 47.1 -8.0 97 97 A V E -AE 9 116A 0 -88,-2.5 -88,-2.6 -2,-0.4 2,-0.4 -0.992 8.3-174.0-136.5 142.6 43.6 49.4 -7.4 98 98 A I E +AE 8 115A 27 17,-1.7 17,-2.1 -2,-0.4 2,-0.3 -0.985 15.7 165.2-135.8 121.6 47.0 48.8 -5.9 99 99 A S E +AE 7 114A 0 -92,-1.5 -92,-2.1 -2,-0.4 2,-0.3 -0.983 13.6 114.7-137.4 148.8 49.3 51.7 -5.3 100 100 A G E -AE 6 113A 0 13,-1.9 13,-2.1 -2,-0.3 2,-0.3 -0.990 41.1-117.7 175.5-174.0 52.5 52.1 -3.2 101 101 A a E -AE 5 112A 0 -96,-1.5 -96,-2.2 -2,-0.3 2,-0.3 -0.955 13.3-145.5-147.4 161.2 56.2 52.7 -3.0 102 102 A E E -AE 4 111A 55 9,-2.2 8,-3.7 -2,-0.3 9,-1.4 -0.964 12.0-168.7-131.4 148.1 59.3 50.8 -2.0 103 103 A V E -AE 3 109A 2 -100,-2.5 -100,-2.4 -2,-0.3 6,-0.2 -0.951 21.7-118.2-134.8 153.6 62.5 52.2 -0.3 104 104 A G > - 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