==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=26-JAN-2011 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER TRANSPORT PROTEIN 17-SEP-10 3OW7 . COMPND 2 MOLECULE: CATION EFFLUX SYSTEM PROTEIN CUSB; . SOURCE 2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI; . AUTHOR C.-C.SU . 593 2 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 35213.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 359 60.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 174 29.3 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 2 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 1 0.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 3 0.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 1 0.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 1 0.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 75 12.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 25 4.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 67 11.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 3 0.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 1 0 1 0 1 0 1 0 0 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 7 10 11 7 1 0 1 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 1 1 6 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 90 A Q 0 0 134 0, 0.0 293,-0.2 0, 0.0 4,-0.0 0.000 360.0 360.0 360.0 83.9 49.3 58.2 -82.2 2 91 A N - 0 0 75 291,-0.9 292,-0.1 1,-0.1 290,-0.0 0.988 360.0-132.6 -65.2 -88.0 48.2 61.2 -80.1 3 92 A L S S+ 0 0 148 290,-0.3 2,-1.5 3,-0.0 -1,-0.1 -0.021 81.4 96.2 149.9 -29.3 50.5 62.0 -77.1 4 93 A G + 0 0 55 2,-0.0 2,-0.3 289,-0.0 -2,-0.0 -0.188 64.9 124.5 -78.6 46.1 48.1 62.4 -74.3 5 94 A V - 0 0 59 -2,-1.5 2,-0.4 287,-0.1 287,-0.1 -0.789 54.4-138.6-111.7 150.1 48.7 58.8 -73.3 6 95 A K E -A 290 0A 129 284,-0.7 284,-1.9 -2,-0.3 2,-0.5 -0.838 13.7-138.0-104.6 145.3 49.8 57.2 -70.1 7 96 A T E +A 289 0A 65 -2,-0.4 282,-0.2 282,-0.2 2,-0.1 -0.898 25.8 170.6-105.5 125.1 52.3 54.3 -69.9 8 97 A A - 0 0 12 280,-4.0 280,-0.3 -2,-0.5 2,-0.2 0.059 18.1-137.6-101.7-142.2 51.7 51.4 -67.5 9 98 A T - 0 0 58 278,-0.3 2,-0.4 -2,-0.1 278,-0.2 -0.859 20.4-100.1 177.9 141.1 53.5 48.1 -67.2 10 99 A V - 0 0 6 276,-2.3 2,-0.4 -2,-0.2 228,-0.2 -0.595 43.2-168.0 -68.5 126.4 53.1 44.4 -66.7 11 100 A T E -B 237 0B 78 226,-2.5 226,-2.8 -2,-0.4 2,-0.3 -0.923 16.7-129.2-117.3 144.5 53.8 43.6 -63.1 12 101 A R E +B 236 0B 138 -2,-0.4 224,-0.2 224,-0.2 272,-0.0 -0.705 58.6 84.1 -89.2 149.5 54.3 40.2 -61.6 13 102 A G E -B 235 0B 14 222,-0.9 222,-3.5 -2,-0.3 2,-0.2 -0.929 66.4 -65.7 151.0-175.3 52.2 39.2 -58.6 14 103 A P - 0 0 70 0, 0.0 2,-0.2 0, 0.0 219,-0.1 -0.492 36.9-113.3-105.8 173.1 48.9 37.9 -57.3 15 104 A L - 0 0 2 218,-0.2 2,-0.6 -2,-0.2 217,-0.3 -0.696 18.9-133.1-106.2 158.0 45.2 39.0 -57.2 16 105 A T - 0 0 68 256,-0.3 2,-0.4 -2,-0.2 215,-0.2 -0.936 17.8-166.6-118.4 113.0 43.0 39.9 -54.2 17 106 A F E -E 230 0C 0 -2,-0.6 213,-2.0 213,-0.5 2,-0.2 -0.764 7.6-156.9 -95.7 142.8 39.5 38.4 -53.9 18 107 A A E +E 229 0C 22 -2,-0.4 2,-0.3 211,-0.2 211,-0.2 -0.707 13.9 172.5-113.2 170.4 37.0 39.8 -51.5 19 108 A Q E -E 228 0C 42 209,-0.6 209,-1.4 -2,-0.2 2,-0.4 -0.933 22.9-135.4-170.9 147.7 34.0 38.4 -49.8 20 109 A S - 0 0 72 -2,-0.3 207,-0.2 207,-0.2 205,-0.1 -0.899 25.9-161.8-116.1 148.0 31.5 39.4 -47.1 21 110 A F - 0 0 29 -2,-0.4 205,-1.0 140,-0.0 2,-0.4 -0.974 25.5-104.6-141.7 146.3 30.4 37.0 -44.4 22 111 A P E -F 225 0C 68 0, 0.0 138,-0.7 0, 0.0 2,-0.5 -0.491 39.1-170.8 -68.4 116.1 27.7 36.3 -41.7 23 112 A A E -FG 224 159C 8 201,-1.7 201,-1.3 -2,-0.4 2,-0.4 -0.943 9.4-167.2-119.0 112.7 29.1 37.2 -38.3 24 113 A N E - G 0 158C 104 134,-1.4 134,-1.7 -2,-0.5 2,-0.2 -0.809 22.3-131.5 -90.6 135.9 27.2 36.3 -35.1 25 114 A V E - G 0 157C 14 -2,-0.4 196,-1.5 197,-0.4 2,-0.3 -0.622 27.2-177.3 -94.3 153.9 28.6 38.0 -32.0 26 115 A S E - G 0 156C 47 130,-3.9 130,-2.1 -2,-0.2 2,-0.6 -0.878 30.7-106.2-145.1 162.6 29.4 36.3 -28.7 27 116 A Y - 0 0 57 -2,-0.3 128,-0.1 128,-0.2 125,-0.1 -0.876 46.0-110.1 -95.4 119.9 30.6 36.6 -25.0 28 117 A N - 0 0 4 -2,-0.6 3,-0.5 126,-0.4 123,-0.4 -0.262 18.0-148.3 -48.8 118.1 34.1 35.3 -24.5 29 118 A E S S+ 0 0 119 1,-0.2 -1,-0.2 2,-0.1 3,-0.2 0.326 95.6 59.3 -71.2 12.2 33.6 32.2 -22.4 30 119 A Y S S+ 0 0 157 1,-0.1 2,-0.9 121,-0.1 -1,-0.2 0.740 87.0 64.5-112.1 -36.3 37.0 33.2 -20.9 31 120 A Q S S+ 0 0 78 -3,-0.5 120,-1.7 120,-0.1 2,-0.3 -0.246 80.7 127.5 -82.1 44.6 36.4 36.7 -19.4 32 121 A Y B -K 150 0D 118 -2,-0.9 2,-0.5 118,-0.2 118,-0.2 -0.820 47.4-154.7-103.3 145.0 34.0 35.1 -17.0 33 122 A A - 0 0 10 116,-2.1 116,-0.4 -2,-0.3 2,-0.4 -0.961 11.9-165.9-120.3 115.8 34.1 35.5 -13.2 34 123 A I - 0 0 65 -2,-0.5 2,-0.5 114,-0.2 114,-0.2 -0.827 11.1-160.3-104.6 142.0 32.6 32.8 -10.9 35 124 A V - 0 0 19 112,-0.7 111,-1.9 -2,-0.4 2,-0.3 -0.973 19.1-171.2-113.7 125.1 31.8 33.0 -7.3 36 125 A Q B -N 145 0E 104 -2,-0.5 2,-1.1 109,-0.2 109,-0.2 -0.876 26.9-116.7-122.5 154.0 31.5 29.5 -5.7 37 126 A A - 0 0 19 107,-1.9 106,-3.6 106,-0.4 107,-0.3 -0.712 21.7-167.0 -96.9 95.8 30.3 28.5 -2.3 38 127 A R S S+ 0 0 136 -2,-1.1 2,-0.3 105,-0.2 -1,-0.2 0.714 71.4 32.1 -56.8 -26.0 33.2 26.8 -0.5 39 128 A A S S- 0 0 17 103,-0.1 2,-1.0 61,-0.1 104,-0.2 -0.906 88.1-103.2-132.3 159.7 31.0 25.5 2.3 40 129 A A + 0 0 34 -2,-0.3 28,-0.3 101,-0.2 101,-0.3 -0.728 66.1 128.4 -87.9 104.3 27.5 24.2 2.7 41 130 A G - 0 0 4 -2,-1.0 99,-2.4 99,-0.5 2,-0.3 -0.630 52.4 -89.3-139.1-170.2 25.5 26.9 4.4 42 131 A F E -OP 65 139F 96 23,-2.8 23,-2.2 -2,-0.2 2,-0.3 -0.761 31.7-131.6-115.0 159.5 22.4 29.1 4.3 43 132 A I E +O 64 0F 24 95,-0.6 2,-0.3 -2,-0.3 21,-0.2 -0.735 22.2 167.9-114.5 155.7 21.7 32.5 2.6 44 133 A D E +O 63 0F 64 19,-2.1 19,-1.3 -2,-0.3 93,-0.1 -0.912 56.7 21.1-151.2 177.6 20.1 35.9 3.6 45 134 A K - 0 0 155 -2,-0.3 91,-0.4 17,-0.2 -2,-0.0 -0.403 55.7-176.6 58.7-138.6 19.8 39.4 2.2 46 135 A V - 0 0 16 89,-0.1 16,-0.5 -2,-0.0 -1,-0.1 -0.104 30.7-123.6 140.6 -30.0 20.5 39.2 -1.6 47 136 A Y - 0 0 129 14,-0.1 2,-1.1 1,-0.1 14,-0.1 0.040 41.7 -67.4 80.2 163.2 20.4 42.8 -2.9 48 137 A P S S+ 0 0 112 0, 0.0 2,-0.3 0, 0.0 -1,-0.1 -0.734 79.1 142.3 -91.7 94.1 18.1 44.0 -5.7 49 138 A L - 0 0 62 -2,-1.1 2,-0.2 84,-0.1 -2,-0.0 -0.905 35.7-159.8-131.5 157.8 19.4 42.3 -8.9 50 139 A T - 0 0 89 -2,-0.3 3,-0.3 79,-0.0 83,-0.2 -0.661 47.3 -89.7-117.4-178.8 18.2 40.6 -12.1 51 140 A V S S+ 0 0 72 1,-0.2 2,-0.3 -2,-0.2 82,-0.0 0.719 115.6 43.0 -68.3 -20.5 20.1 38.2 -14.4 52 141 A G S S+ 0 0 58 78,-0.1 -1,-0.2 76,-0.0 2,-0.2 -0.675 78.9 122.7-133.5 85.0 21.7 40.9 -16.5 53 142 A D - 0 0 43 -2,-0.3 76,-1.7 -3,-0.3 2,-0.3 -0.613 53.5 -96.1-127.6-175.6 23.1 44.0 -14.7 54 143 A K E -L 128 0D 178 74,-0.2 2,-0.3 -2,-0.2 74,-0.2 -0.725 27.2-160.6-103.3 156.1 26.3 46.0 -14.3 55 144 A V E -L 127 0D 36 72,-1.4 72,-0.5 -2,-0.3 4,-0.1 -0.952 12.2-156.9-131.6 156.6 28.9 45.8 -11.5 56 145 A Q S S- 0 0 144 -2,-0.3 -1,-0.1 70,-0.2 71,-0.1 0.871 87.2 -28.0 -99.0 -48.5 31.6 48.3 -10.3 57 146 A K S S- 0 0 153 69,-0.1 2,-0.3 67,-0.0 69,-0.2 0.175 96.5 -91.2-147.2 4.6 34.2 46.2 -8.6 58 147 A G + 0 0 1 69,-0.1 65,-0.6 2,-0.1 68,-0.1 -0.454 63.5 177.2 106.6 -57.8 31.9 43.4 -7.4 59 148 A T - 0 0 50 64,-0.4 64,-1.7 -2,-0.3 2,-0.3 0.030 46.6 -17.5 49.3-159.1 31.1 44.9 -4.0 60 149 A P E + Q 0 122F 55 0, 0.0 2,-0.4 0, 0.0 62,-0.2 -0.612 59.6 169.5 -94.3 136.0 28.6 43.1 -1.8 61 150 A L E - Q 0 121F 31 60,-0.6 60,-0.7 -2,-0.3 -14,-0.1 -0.976 55.7 -32.5-130.8 143.1 26.2 40.4 -2.9 62 151 A L E - 0 0 18 -16,-0.5 59,-0.9 -2,-0.4 2,-0.3 0.638 63.8-132.6 19.3 128.4 24.0 38.1 -0.7 63 152 A D E -OQ 44 120F 16 -19,-1.3 -19,-2.1 57,-0.2 57,-0.2 -0.815 16.2-152.1-110.0 145.8 25.4 37.1 2.7 64 153 A L E -OQ 43 119F 31 55,-2.3 55,-1.8 -2,-0.3 2,-0.3 -0.618 8.3-158.5-108.4 171.0 25.5 33.6 4.2 65 154 A T E -O 42 0F 49 -23,-2.2 -23,-2.8 53,-0.2 53,-0.1 -0.975 15.7-162.6-142.0 145.2 25.5 32.0 7.6 66 155 A I - 0 0 14 -2,-0.3 4,-0.4 -25,-0.2 -25,-0.1 -0.989 20.0-164.4-137.2 143.4 26.8 28.5 8.4 67 156 A P S > S+ 0 0 62 0, 0.0 4,-1.3 0, 0.0 -1,-0.1 0.757 78.9 72.4 -90.4 -32.6 26.5 26.0 11.2 68 157 A D H > S+ 0 0 111 -28,-0.3 4,-0.6 1,-0.2 -27,-0.1 0.710 102.5 39.9 -69.1 -20.8 29.3 23.6 10.5 69 158 A W H > S+ 0 0 19 2,-0.2 4,-3.8 3,-0.1 5,-0.3 0.816 103.4 64.0 -96.8 -36.4 32.1 25.8 11.5 70 159 A V H > S+ 0 0 40 -4,-0.4 4,-1.6 1,-0.2 -2,-0.2 0.879 103.0 50.9 -60.0 -39.6 30.7 27.5 14.6 71 160 A E H X S+ 0 0 122 -4,-1.3 4,-1.2 2,-0.2 -1,-0.2 0.966 117.1 39.6 -60.6 -51.3 30.5 24.2 16.4 72 161 A A H X S+ 0 0 30 -4,-0.6 4,-1.1 1,-0.2 3,-0.3 0.917 115.0 50.3 -66.3 -48.7 34.1 23.4 15.6 73 162 A Q H X S+ 0 0 1 -4,-3.8 4,-2.2 1,-0.2 -1,-0.2 0.776 102.4 62.7 -63.0 -27.3 35.5 26.9 16.0 74 163 A S H X S+ 0 0 49 -4,-1.6 4,-2.3 -5,-0.3 5,-0.4 0.929 95.9 57.2 -66.7 -45.5 33.9 27.3 19.4 75 164 A E H X S+ 0 0 99 -4,-1.2 4,-1.5 1,-0.3 -1,-0.2 0.900 107.8 50.0 -49.2 -42.9 35.9 24.4 20.9 76 165 A Y H X S+ 0 0 1 -4,-1.1 4,-1.4 2,-0.2 3,-0.5 0.953 105.5 57.8 -55.3 -50.6 38.9 26.4 19.8 77 166 A L H >X S+ 0 0 27 -4,-2.2 4,-0.7 1,-0.3 3,-0.5 0.887 111.2 39.1 -52.4 -46.5 37.3 29.4 21.5 78 167 A L H 3X S+ 0 0 98 -4,-2.3 4,-3.0 1,-0.2 -1,-0.3 0.726 107.8 64.7 -75.4 -21.0 37.2 27.7 24.9 79 168 A L H 3< S+ 0 0 36 -4,-1.5 -1,-0.2 -3,-0.5 -2,-0.2 0.759 101.8 50.9 -70.8 -24.2 40.6 26.1 24.3 80 169 A R H << S+ 0 0 109 -4,-1.4 -1,-0.2 -3,-0.5 -2,-0.2 0.743 112.2 46.1 -82.6 -27.0 41.9 29.6 24.3 81 170 A E H < S+ 0 0 147 -4,-0.7 -2,-0.2 -5,-0.2 -3,-0.1 0.955 116.4 45.3 -75.8 -55.2 40.3 30.3 27.6 82 171 A T S < S- 0 0 79 -4,-3.0 2,-0.3 1,-0.2 0, 0.0 -0.180 116.1 -62.0 -78.9 176.1 41.3 27.1 29.3 83 172 A G + 0 0 78 -2,-0.0 2,-0.3 1,-0.0 -1,-0.2 -0.466 68.0 151.0 -64.9 122.5 44.9 25.6 29.1 84 173 A G - 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