==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=15-MAY-2011 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER ANTIFREEZE PROTEIN 06-OCT-10 3P4G . COMPND 2 MOLECULE: ANTIFREEZE PROTEIN; . SOURCE 2 ORGANISM_SCIENTIFIC: MARINOMONAS PRIMORYENSIS; . AUTHOR C.P.GARNHAM,R.L.CAMPBELL,P.L.DAVIES . 1227 4 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 42447.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 912 74.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 522 42.5 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 20 1.6 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 4 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 1 0.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 178 14.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 36 2.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 34 2.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 2 0 2 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 48 26 22 52 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 4 4 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 23 A H 0 0 105 0, 0.0 18,-3.0 0, 0.0 2,-0.4 0.000 360.0 360.0 360.0 119.9 -3.0 -44.3 5.5 2 24 A S E +a 19 0A 109 16,-0.2 2,-0.3 18,-0.1 18,-0.2 -0.903 360.0 168.2-110.4 135.9 -6.1 -46.5 5.8 3 25 A F E -a 20 0A 47 16,-2.8 18,-2.6 -2,-0.4 19,-0.2 -0.967 26.0-166.9-135.9 152.1 -9.4 -45.8 4.0 4 26 A D S S+ 0 0 114 1,-0.4 18,-2.1 -2,-0.3 2,-0.8 0.674 94.3 16.4 -94.9 -85.8 -12.6 -47.7 3.4 5 27 A H S S- 0 0 105 17,-0.2 2,-0.7 16,-0.1 -1,-0.4 -0.761 88.1-142.5 -84.9 112.4 -14.3 -45.6 0.7 6 28 A Y B +p 24 0B 0 17,-2.8 19,-3.0 -2,-0.8 20,-0.2 -0.691 33.7 160.9 -80.2 116.7 -11.4 -43.4 -0.7 7 29 A I - 0 0 124 -2,-0.7 20,-0.1 1,-0.3 -1,-0.1 0.114 63.6 -50.6-123.9 19.7 -12.8 -40.0 -1.6 8 30 A G + 0 0 39 18,-0.2 -1,-0.3 1,-0.0 2,-0.3 0.007 56.5 169.4 121.6 135.6 -9.5 -38.0 -1.7 9 31 A S - 0 0 54 18,-0.5 2,-0.4 -3,-0.1 20,-0.4 -0.952 31.0-114.1-165.7 159.0 -6.5 -37.4 0.4 10 32 A A - 0 0 84 -2,-0.3 2,-0.1 18,-0.1 20,-0.1 -0.863 30.5-177.1-104.0 136.8 -3.1 -35.7 0.1 11 33 A F - 0 0 16 18,-0.6 2,-0.3 -2,-0.4 19,-0.1 -0.277 6.9-156.0-113.3-164.1 0.2 -37.7 0.3 12 34 A D - 0 0 79 18,-0.4 4,-0.3 2,-0.2 -1,-0.0 -0.872 39.3 -63.0-158.7-164.1 3.9 -36.7 0.1 13 35 A A S S+ 0 0 36 -2,-0.3 21,-0.1 18,-0.1 17,-0.0 0.525 93.5 100.8 -78.5 -6.2 7.5 -37.5 -0.6 14 36 A S S S- 0 0 59 1,-0.1 2,-1.4 19,-0.1 -2,-0.2 -0.227 94.7 -91.9 -70.4 165.6 7.7 -40.2 2.1 15 37 A N - 0 0 62 18,-0.1 20,-0.2 20,-0.1 18,-0.1 -0.718 59.0-153.8 -75.1 91.8 7.5 -43.9 1.1 16 38 A N - 0 0 4 -2,-1.4 20,-2.8 16,-0.3 2,-0.4 -0.341 20.3-158.1 -69.4 153.6 3.7 -44.1 1.6 17 39 A N E - b 0 36A 72 18,-0.2 2,-0.5 -2,-0.1 20,-0.2 -0.959 16.7-175.3-130.9 110.3 1.6 -47.2 2.4 18 40 A V E - b 0 37A 0 18,-2.6 20,-2.5 -2,-0.4 2,-0.5 -0.955 5.9-172.7-113.1 123.6 -2.0 -46.7 1.4 19 41 A A E -ab 2 38A 36 -18,-3.0 -16,-2.8 -2,-0.5 2,-0.5 -0.974 3.5-179.7-118.6 117.5 -4.7 -49.3 2.2 20 42 A V E -ab 3 39A 2 18,-3.2 20,-2.8 -2,-0.5 21,-0.4 -0.965 10.6-175.1-116.6 113.5 -8.1 -48.9 0.7 21 43 A T S S+ 0 0 84 -18,-2.6 -17,-0.2 -2,-0.5 -16,-0.1 0.727 75.7 28.7 -81.5 -19.3 -10.7 -51.6 1.6 22 44 A G S S- 0 0 14 -18,-2.1 19,-1.5 -19,-0.2 18,-0.2 -0.045 106.8 -39.9-113.2-141.0 -13.4 -50.1 -0.7 23 45 A N - 0 0 52 18,-0.2 -17,-2.8 17,-0.1 2,-0.7 -0.479 41.8-125.8 -91.5 157.0 -13.4 -48.1 -4.0 24 46 A V B +pq 6 43B 0 18,-2.9 20,-2.2 -19,-0.2 -17,-0.2 -0.917 35.7 164.0 -95.6 112.2 -11.2 -45.2 -5.2 25 47 A S + 0 0 34 -19,-3.0 2,-0.3 -2,-0.7 -18,-0.1 0.196 58.8 43.5-120.4 15.3 -13.5 -42.4 -6.1 26 48 A A S S- 0 0 19 -20,-0.2 -18,-0.2 20,-0.1 18,-0.1 -0.939 101.2 -70.9-144.0 171.9 -11.1 -39.5 -6.3 27 49 A T - 0 0 72 -2,-0.3 -18,-0.5 -20,-0.1 2,-0.4 -0.335 44.6-162.0 -63.1 143.6 -7.6 -38.9 -7.6 28 50 A L E +b 55 0C 0 26,-2.8 28,-3.0 -20,-0.1 2,-0.4 -0.983 13.6 172.3-132.8 116.4 -4.9 -40.7 -5.6 29 51 A N E -b 56 0C 44 -2,-0.4 -18,-0.6 -20,-0.4 28,-0.2 -0.917 16.5-176.1-133.1 108.4 -1.4 -39.5 -6.0 30 52 A V - 0 0 0 26,-2.1 -18,-0.4 -2,-0.4 -14,-0.3 0.522 29.4-168.7 -89.3 0.0 1.3 -40.9 -3.8 31 53 A L + 0 0 14 25,-0.5 4,-0.2 -20,-0.1 -1,-0.2 -0.053 44.6 4.9 61.1-151.1 4.0 -38.6 -5.3 32 54 A A S S+ 0 0 9 742,-0.2 -16,-0.3 26,-0.1 -20,-0.2 -0.152 98.4 56.9 -67.0 166.7 7.7 -39.1 -4.6 33 55 A G S S- 0 0 15 -18,-0.1 2,-1.0 2,-0.1 -18,-0.1 -0.144 98.8 -69.2 96.7 163.1 9.1 -42.0 -2.7 34 56 A D - 0 0 25 26,-0.2 536,-0.3 -21,-0.1 2,-0.3 -0.800 68.3-171.7 -93.7 99.6 8.7 -45.7 -3.2 35 57 A D - 0 0 0 -2,-1.0 28,-3.0 22,-0.3 2,-0.4 -0.667 21.7-151.9-103.0 150.2 5.0 -46.0 -2.3 36 58 A K E -bc 17 63A 38 -20,-2.8 -18,-2.6 -2,-0.3 2,-0.4 -0.971 14.7-177.6-120.2 131.0 2.8 -49.0 -1.9 37 59 A V E -bc 18 64A 0 26,-2.5 28,-2.7 -2,-0.4 2,-0.4 -1.000 2.6-176.0-129.2 132.3 -0.9 -48.7 -2.6 38 60 A S E -bc 19 65A 27 -20,-2.5 -18,-3.2 -2,-0.4 2,-0.4 -0.994 1.4-178.8-135.8 124.2 -3.5 -51.6 -2.1 39 61 A I E -bc 20 66A 0 26,-2.1 28,-2.8 -2,-0.4 29,-0.4 -0.989 15.7-169.1-128.0 121.6 -7.1 -51.3 -3.1 40 62 A D S S+ 0 0 84 -20,-2.8 -19,-0.2 -2,-0.4 -17,-0.1 0.700 78.3 43.2 -85.3 -21.2 -9.6 -54.1 -2.5 41 63 A G S S- 0 0 11 -19,-1.5 27,-1.8 -21,-0.4 2,-0.3 0.124 102.5 -57.0 -96.6-142.3 -12.3 -52.4 -4.7 42 64 A N - 0 0 49 26,-0.2 -18,-2.9 25,-0.1 2,-0.8 -0.716 30.8-126.9-106.4 149.6 -12.2 -50.6 -8.0 43 65 A V B -qr 24 70B 0 26,-2.4 28,-3.6 -2,-0.3 29,-0.5 -0.892 47.5-141.6 -87.0 112.4 -10.3 -47.6 -9.4 44 66 A E - 0 0 64 -20,-2.2 2,-2.3 -2,-0.8 10,-0.0 -0.243 19.6 -97.2 -81.5 162.5 -13.3 -45.7 -10.6 45 67 A D - 0 0 130 27,-0.1 2,-0.2 25,-0.0 27,-0.2 -0.473 53.0-173.5 -72.5 67.7 -13.9 -43.6 -13.7 46 68 A V - 0 0 27 -2,-2.3 2,-0.4 -22,-0.1 27,-0.2 -0.493 32.9-104.9 -67.3 131.0 -13.1 -40.3 -11.8 47 69 A L + 0 0 152 -2,-0.2 2,-0.3 25,-0.1 -20,-0.1 -0.475 60.1 149.5 -63.2 114.2 -13.8 -37.2 -13.9 48 70 A V - 0 0 48 -2,-0.4 3,-0.1 4,-0.1 6,-0.1 -0.911 50.9 -85.0-136.6 162.5 -10.4 -35.8 -14.9 49 71 A A S > S- 0 0 32 -2,-0.3 3,-2.0 1,-0.2 -1,-0.1 -0.191 71.2 -63.3 -60.4 164.9 -9.1 -33.9 -18.0 50 72 A A T 3 S+ 0 0 45 1,-0.3 -1,-0.2 1040,-0.1 3,-0.1 -0.242 121.9 3.1 -53.4 133.1 -8.1 -35.8 -21.1 51 73 A N T 3 S+ 0 0 122 1,-0.2 2,-0.3 -3,-0.1 -1,-0.3 0.482 93.9 138.5 67.9 9.0 -5.1 -38.2 -20.4 52 74 A V < - 0 0 58 -3,-2.0 21,-0.3 1,-0.1 2,-0.3 -0.629 54.7-118.7 -82.0 140.6 -4.9 -37.3 -16.8 53 75 A A - 0 0 18 -2,-0.3 21,-0.2 1,-0.2 3,-0.2 -0.588 15.7-163.3 -80.0 139.1 -4.2 -40.1 -14.3 54 76 A V S S+ 0 0 3 19,-1.9 -26,-2.8 1,-0.3 2,-0.5 0.877 90.5 32.9 -76.4 -44.1 -6.8 -41.0 -11.7 55 77 A L E S-bc 28 74C 0 18,-2.3 20,-2.6 -28,-0.2 2,-0.5 -0.964 74.9-177.2-118.7 119.2 -4.2 -43.0 -9.8 56 78 A D E -bc 29 75C 13 -28,-3.0 -26,-2.1 -2,-0.5 -25,-0.5 -0.980 17.2-172.6-122.3 118.7 -0.6 -41.7 -10.0 57 79 A M - 0 0 1 18,-2.8 -22,-0.3 -2,-0.5 19,-0.2 0.660 38.2-161.1 -83.7 -15.9 2.2 -43.7 -8.2 58 80 A G - 0 0 2 17,-0.6 4,-0.2 -28,-0.1 -1,-0.2 -0.111 38.2 -18.5 71.8-169.6 4.6 -40.9 -9.1 59 81 A T S S+ 0 0 4 696,-0.2 20,-0.1 18,-0.2 2,-0.1 -0.274 103.9 61.0 -59.2 157.4 8.4 -40.9 -9.2 60 82 A G S S- 0 0 13 -28,-0.1 2,-1.0 -26,-0.1 -26,-0.2 -0.289 99.7 -65.3 109.2 164.1 9.9 -43.8 -7.4 61 83 A N - 0 0 27 18,-0.2 490,-0.4 -2,-0.1 2,-0.2 -0.804 69.6-174.8 -90.5 94.3 9.9 -47.6 -7.5 62 84 A D - 0 0 1 -2,-1.0 20,-2.8 14,-0.2 2,-0.5 -0.630 21.9-150.1-103.7 156.2 6.2 -48.0 -6.6 63 85 A Q E -cd 36 82A 26 -28,-3.0 -26,-2.5 -2,-0.2 2,-0.4 -0.987 15.1-174.3-132.4 121.3 4.0 -51.1 -5.9 64 86 A L E -cd 37 83A 4 18,-2.8 20,-2.0 -2,-0.5 2,-0.4 -0.948 8.4-176.2-120.7 133.6 0.3 -51.2 -6.7 65 87 A Y E +cd 38 84A 81 -28,-2.7 -26,-2.1 -2,-0.4 2,-0.4 -1.000 3.8 176.7-125.2 128.0 -2.3 -53.9 -5.9 66 88 A V E -cd 39 85A 0 18,-2.6 20,-3.0 -2,-0.4 21,-0.4 -0.975 11.0-172.5-125.6 120.3 -5.9 -53.5 -7.1 67 89 A A S S+ 0 0 26 -28,-2.8 -27,-0.2 -2,-0.4 -25,-0.1 0.743 73.8 43.2 -80.8 -25.6 -8.3 -56.5 -6.4 68 90 A G S S- 0 0 13 -27,-1.8 19,-1.7 -29,-0.4 2,-0.2 0.148 103.5 -56.2 -95.2-145.3 -11.2 -55.1 -8.5 69 91 A D - 0 0 57 18,-0.2 -26,-2.4 17,-0.1 2,-0.6 -0.570 37.9-131.0-101.3 162.2 -11.4 -53.4 -11.9 70 92 A V B +rs 43 89B 0 18,-2.5 20,-3.0 -28,-0.2 21,-0.6 -0.967 36.1 155.5-118.5 114.1 -9.6 -50.3 -13.2 71 93 A L + 0 0 64 -28,-3.6 2,-0.3 -2,-0.6 -27,-0.2 0.295 62.0 61.5-119.2 0.1 -11.9 -47.8 -14.9 72 94 A G S S- 0 0 2 -29,-0.5 19,-0.2 -27,-0.2 -27,-0.1 -0.957 98.8 -84.8-128.5 149.3 -9.8 -44.7 -14.3 73 95 A K - 0 0 41 -21,-0.3 -18,-2.3 -2,-0.3 -19,-1.9 -0.198 36.6-154.9 -61.2 137.9 -6.3 -44.1 -15.7 74 96 A I E -cd 55 93C 0 18,-2.2 20,-2.5 -20,-0.2 2,-0.5 -0.922 9.2-174.5-108.6 133.4 -3.2 -45.4 -13.9 75 97 A D E -cd 56 94C 11 -20,-2.6 -18,-2.8 -2,-0.4 -17,-0.6 -0.986 9.6-173.4-124.4 119.2 0.2 -43.9 -14.3 76 98 A A - 0 0 0 18,-3.0 -14,-0.2 -2,-0.5 19,-0.2 0.620 33.6-159.7 -82.2 -11.1 3.1 -45.7 -12.5 77 99 A G - 0 0 3 17,-0.6 4,-0.2 -20,-0.1 -1,-0.2 -0.080 38.8 -20.8 65.5-169.0 5.4 -42.8 -13.4 78 100 A T S S+ 0 0 1 658,-0.4 20,-0.1 18,-0.2 2,-0.1 -0.201 102.0 61.2 -61.0 163.4 9.2 -42.9 -13.6 79 101 A G S S- 0 0 7 -20,-0.1 2,-1.1 -18,-0.1 -18,-0.2 -0.141 100.2 -62.8 100.8 167.0 10.9 -45.7 -11.6 80 102 A N - 0 0 28 18,-0.2 450,-0.4 20,-0.1 2,-0.3 -0.765 68.4-170.8 -92.1 92.8 10.8 -49.5 -11.7 81 103 A D - 0 0 1 -2,-1.1 20,-3.2 14,-0.3 2,-0.5 -0.672 21.2-152.1 -99.3 151.5 7.2 -50.0 -10.8 82 104 A E E -de 63 101A 32 -20,-2.8 -18,-2.8 -2,-0.3 2,-0.4 -0.979 16.6-177.6-125.7 117.6 5.2 -53.2 -10.0 83 105 A I E +de 64 102A 5 18,-3.5 20,-2.2 -2,-0.5 2,-0.4 -0.976 7.4 179.9-120.8 132.1 1.4 -53.2 -10.8 84 106 A Y E +de 65 103A 92 -20,-2.0 -18,-2.6 -2,-0.4 2,-0.5 -0.918 3.7 173.7-133.9 111.4 -1.0 -56.1 -10.0 85 107 A I E -de 66 104A 0 18,-3.2 20,-3.2 -2,-0.4 21,-0.3 -0.972 13.6-167.1-116.3 120.4 -4.6 -55.7 -11.0 86 108 A K + 0 0 133 -20,-3.0 -19,-0.2 -2,-0.5 -17,-0.1 0.849 69.1 48.9 -75.4 -34.9 -6.8 -58.8 -10.5 87 109 A G S S- 0 0 13 -19,-1.7 19,-2.1 -21,-0.4 2,-0.2 0.167 100.2 -56.5 -91.8-152.3 -9.8 -57.6 -12.5 88 110 A D - 0 0 69 18,-0.2 -18,-2.5 17,-0.2 2,-0.5 -0.608 40.5-143.7 -95.3 151.9 -10.5 -56.0 -15.9 89 111 A V B +st 70 108B 0 18,-2.8 20,-3.5 -2,-0.2 21,-0.8 -0.973 28.2 161.7-120.7 121.6 -8.9 -52.8 -17.1 90 112 A S + 0 0 60 -20,-3.0 2,-0.3 -2,-0.5 -19,-0.2 0.187 60.0 67.3-120.8 7.8 -11.0 -50.4 -19.2 91 113 A A S S- 0 0 14 -21,-0.6 19,-0.3 -19,-0.2 -19,-0.1 -0.829 95.6 -77.6-122.1 168.9 -8.9 -47.3 -18.8 92 114 A A - 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