==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=19-FEB-2012 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 05-NOV-10 3PIB . COMPND 2 MOLECULE: EQFP578 FLUORESCENT PROTEIN; . SOURCE 2 ORGANISM_SCIENTIFIC: ENTACMAEA QUADRICOLOR; . AUTHOR S.PLETNEV,N.V.PLETNEVA,V.Z.PLETNEV . 913 8 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 33939.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 626 68.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 52 5.7 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 408 44.7 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 12 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 56 6.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 86 9.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 26 2.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 12 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 4 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 4 0 0 8 0 4 4 8 4 12 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 A S 0 0 164 0, 0.0 2,-0.5 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 -27.6 5.9 -13.2 -4.0 2 2 A S > + 0 0 18 3,-0.2 3,-1.9 1,-0.1 79,-0.0 -0.683 360.0 176.6-120.7 76.1 9.1 -13.1 -6.1 3 3 A E T 3 S+ 0 0 164 -2,-0.5 -1,-0.1 1,-0.3 0, 0.0 0.744 74.2 61.8 -54.3 -33.5 8.1 -14.9 -9.3 4 4 A L T 3 S+ 0 0 79 75,-0.1 2,-0.8 78,-0.1 -1,-0.3 0.752 91.2 74.2 -68.8 -24.7 11.4 -14.2 -11.1 5 5 A I S < S- 0 0 4 -3,-1.9 -3,-0.2 74,-0.1 -1,-0.1 -0.839 76.3-161.7 -95.4 108.7 13.3 -16.2 -8.5 6 6 A K - 0 0 105 -2,-0.8 29,-0.2 2,-0.2 -2,-0.1 -0.288 35.1 -98.0 -86.5 171.6 12.6 -19.9 -9.1 7 7 A E S S+ 0 0 124 1,-0.2 2,-0.4 26,-0.1 28,-0.3 0.797 116.4 32.6 -58.9 -32.0 13.0 -22.9 -6.8 8 8 A N S S+ 0 0 99 25,-0.2 2,-0.3 26,-0.1 -2,-0.2 -0.997 76.6 175.6-126.3 134.3 16.3 -23.6 -8.6 9 9 A M E -A 32 0A 12 23,-2.3 23,-2.2 -2,-0.4 2,-0.3 -0.985 19.2-143.3-138.9 149.4 18.6 -20.9 -10.0 10 10 A H E -A 31 0A 63 101,-0.5 103,-2.4 -2,-0.3 2,-0.3 -0.722 16.5-162.1-102.7 158.8 21.9 -20.6 -11.6 11 11 A M E -Ab 30 113A 0 19,-2.0 19,-2.4 -2,-0.3 2,-0.3 -0.971 8.8-162.9-139.8 153.3 24.5 -17.9 -11.1 12 12 A K E -Ab 29 114A 85 101,-2.2 103,-2.8 -2,-0.3 2,-0.4 -0.991 14.8-166.7-134.0 143.3 27.6 -16.6 -12.9 13 13 A L E -Ab 28 115A 10 15,-2.4 15,-1.8 -2,-0.3 2,-0.4 -0.971 15.9-168.6-136.3 145.7 30.3 -14.4 -11.4 14 14 A Y E -Ab 27 116A 82 101,-2.1 103,-2.5 -2,-0.4 2,-0.4 -0.995 16.6-179.6-128.1 135.8 33.2 -12.2 -12.5 15 15 A M E +Ab 26 117A 0 11,-2.5 11,-2.4 -2,-0.4 2,-0.4 -0.999 9.1 178.4-137.0 136.9 35.6 -10.8 -9.9 16 16 A E E +Ab 25 118A 34 101,-2.1 103,-2.2 -2,-0.4 2,-0.3 -0.992 22.5 132.8-134.0 146.5 38.7 -8.6 -10.2 17 17 A G E -Ab 24 119A 11 7,-2.5 7,-2.4 -2,-0.4 2,-0.4 -0.990 41.9-120.9-170.2-179.3 40.8 -7.4 -7.4 18 18 A T E -Ab 23 120A 6 101,-1.8 103,-3.0 -2,-0.3 2,-0.4 -0.995 12.2-165.9-138.6 134.9 44.1 -6.7 -5.6 19 19 A V E > S-Ab 22 121A 2 3,-2.1 3,-2.2 -2,-0.4 2,-0.8 -0.992 74.9 -38.7-124.0 123.1 45.2 -8.0 -2.2 20 20 A N T 3 S- 0 0 7 101,-2.4 303,-0.1 -2,-0.4 305,-0.1 -0.611 130.1 -28.0 62.6-106.6 48.3 -6.1 -0.9 21 21 A N T 3 S+ 0 0 85 -2,-0.8 2,-0.6 317,-0.1 -1,-0.3 0.022 115.5 108.7-115.5 18.7 50.1 -5.7 -4.2 22 22 A H E < -A 19 0A 52 -3,-2.2 -3,-2.1 2,-0.0 2,-0.2 -0.878 51.5-157.3-115.3 115.5 48.8 -8.8 -5.9 23 23 A H E +A 18 0A 96 -2,-0.6 2,-0.3 -5,-0.2 -5,-0.2 -0.566 21.8 158.6 -87.0 145.3 46.3 -8.5 -8.8 24 24 A F E -A 17 0A 2 -7,-2.4 -7,-2.5 -2,-0.2 2,-0.3 -0.980 26.6-148.6-157.4 167.0 43.9 -11.3 -9.8 25 25 A K E -AC 16 47A 54 22,-2.1 21,-2.2 -2,-0.3 22,-1.6 -0.992 15.3-173.7-140.2 144.7 40.7 -12.2 -11.5 26 26 A C E -AC 15 45A 0 -11,-2.4 -11,-2.5 -2,-0.3 2,-0.3 -0.955 7.1-166.0-133.4 155.4 38.2 -15.0 -10.8 27 27 A T E -AC 14 44A 51 17,-2.3 17,-3.0 -2,-0.3 2,-0.3 -0.934 11.2-176.6-128.4 161.9 35.1 -16.4 -12.4 28 28 A S E -AC 13 43A 4 -15,-1.8 -15,-2.4 -2,-0.3 2,-0.4 -0.998 17.5-156.5-155.8 156.4 32.5 -18.8 -11.1 29 29 A E E +AC 12 42A 88 13,-2.0 13,-3.1 -2,-0.3 2,-0.3 -0.982 26.7 167.6-125.0 145.4 29.4 -20.7 -11.9 30 30 A G E -AC 11 41A 7 -19,-2.4 -19,-2.0 -2,-0.4 2,-0.3 -0.928 22.4-159.2-149.7 171.1 26.9 -21.8 -9.4 31 31 A E E +AC 10 40A 136 9,-2.1 9,-2.7 -2,-0.3 2,-0.3 -0.985 21.6 152.0-148.9 154.6 23.5 -23.2 -8.7 32 32 A G E -AC 9 39A 10 -23,-2.2 -23,-2.3 -2,-0.3 7,-0.3 -0.970 46.9-100.1-166.3-176.4 21.2 -23.2 -5.6 33 33 A K E >>> - C 0 38A 81 5,-2.4 5,-1.4 -2,-0.3 4,-1.1 -0.905 30.5-164.3-120.4 95.5 17.8 -23.3 -4.1 34 34 A P T 345S+ 0 0 0 0, 0.0 34,-0.4 0, 0.0 3,-0.2 0.809 86.1 45.3 -58.0 -37.4 16.9 -19.7 -3.2 35 35 A Y T 345S+ 0 0 75 -28,-0.3 -28,-0.1 1,-0.2 35,-0.1 0.681 113.2 49.9 -82.0 -18.1 14.0 -20.5 -0.9 36 36 A E T <45S- 0 0 100 -3,-1.0 33,-0.3 -29,-0.2 -1,-0.2 0.542 108.6-126.3 -92.0 -8.9 15.9 -23.3 1.0 37 37 A G T <5 + 0 0 0 -4,-1.1 178,-2.0 1,-0.2 2,-0.3 0.747 66.3 122.6 72.4 27.7 18.9 -21.0 1.5 38 38 A T E < +CD 33 214A 27 -5,-1.4 -5,-2.4 176,-0.2 2,-0.3 -0.892 27.3 155.5-123.5 151.7 21.4 -23.3 -0.1 39 39 A Q E -CD 32 213A 12 174,-1.3 174,-2.0 -2,-0.3 2,-0.4 -0.970 25.2-147.6-167.7 153.9 23.9 -22.9 -2.9 40 40 A T E -CD 31 212A 44 -9,-2.7 -9,-2.1 -2,-0.3 2,-0.4 -0.998 12.1-165.7-129.1 135.5 27.2 -24.2 -4.4 41 41 A M E -CD 30 211A 7 170,-2.5 170,-2.5 -2,-0.4 2,-0.6 -0.969 9.9-153.0-121.1 137.3 29.6 -22.1 -6.4 42 42 A K E -CD 29 210A 105 -13,-3.1 -13,-2.0 -2,-0.4 2,-0.4 -0.954 25.0-164.2-110.1 116.7 32.5 -23.5 -8.4 43 43 A I E -CD 28 209A 0 166,-3.3 166,-2.2 -2,-0.6 2,-0.4 -0.872 13.8-167.7-117.3 138.0 35.3 -20.9 -8.6 44 44 A K E -CD 27 208A 122 -17,-3.0 -17,-2.3 -2,-0.4 2,-0.7 -0.977 19.8-141.1-122.7 131.6 38.3 -20.5 -10.8 45 45 A V E +C 26 0A 18 162,-2.4 -19,-0.3 -2,-0.4 3,-0.1 -0.846 28.2 174.4 -82.8 114.0 41.2 -18.1 -10.4 46 46 A V E + 0 0 79 -21,-2.2 2,-0.3 -2,-0.7 -20,-0.2 0.597 61.4 24.4-105.2 -14.5 41.8 -17.1 -14.0 47 47 A E E S+C 25 0A 107 -22,-1.6 -22,-2.1 1,-0.0 -1,-0.3 -0.984 118.7 23.5-146.3 141.7 44.5 -14.5 -13.7 48 48 A G S S- 0 0 29 -2,-0.3 -2,-0.1 1,-0.3 -22,-0.0 0.552 89.7-175.0 85.3 7.4 47.0 -14.0 -11.0 49 49 A G + 0 0 42 -24,-0.1 -1,-0.3 2,-0.1 2,-0.1 -0.561 53.7 45.6 -91.5 157.6 47.0 -17.5 -9.8 50 50 A P S S- 0 0 73 0, 0.0 156,-0.1 0, 0.0 155,-0.1 0.595 87.1-140.0 -68.0 148.4 48.2 -19.1 -7.6 51 51 A L - 0 0 5 154,-0.4 4,-0.1 -2,-0.1 -2,-0.1 -0.515 5.5-139.2 -76.3 144.4 47.4 -16.4 -5.1 52 52 A P S S+ 0 0 43 0, 0.0 2,-0.3 0, 0.0 81,-0.1 0.443 83.2 65.0 -83.5 0.5 49.9 -15.7 -2.4 53 53 A F S S- 0 0 1 79,-0.1 78,-0.2 80,-0.0 -2,-0.1 -0.893 99.8 -85.2-119.3 154.7 47.2 -15.4 0.3 54 54 A A > - 0 0 0 76,-1.8 3,-1.7 -2,-0.3 4,-0.2 -0.338 31.8-137.2 -55.8 128.2 44.8 -17.9 1.8 55 55 A F G > S+ 0 0 0 1,-0.3 3,-2.6 2,-0.2 4,-0.5 0.757 93.1 84.3 -62.4 -25.1 41.7 -18.1 -0.4 56 56 A D G > S+ 0 0 8 1,-0.3 3,-1.1 2,-0.2 4,-0.4 0.785 79.3 64.8 -46.8 -31.2 39.5 -18.1 2.8 57 57 A I G < S+ 0 0 2 -3,-1.7 -1,-0.3 1,-0.2 -2,-0.2 0.669 103.4 47.0 -71.6 -14.6 39.7 -14.3 2.8 58 58 A L G X> S+ 0 0 0 -3,-2.6 3,-1.9 -4,-0.2 4,-0.6 0.481 84.0 98.2 -95.4 -8.7 37.7 -14.2 -0.5 59 59 A A G X4 S+ 0 0 4 -3,-1.1 3,-1.0 -4,-0.5 -2,-0.1 0.850 80.7 48.8 -57.6 -43.5 35.0 -16.6 0.6 60 60 A T G 34 S+ 0 0 33 -4,-0.4 -1,-0.3 1,-0.2 -2,-0.1 0.541 102.3 66.0 -74.0 -6.4 32.3 -14.1 1.6 61 61 A S G <4 0 0 1 -3,-1.9 -1,-0.2 1,-0.2 -2,-0.2 0.643 360.0 360.0 -92.4 -11.6 32.8 -12.2 -1.6 62 62 A F << 0 0 10 -3,-1.0 -1,-0.2 -4,-0.6 -2,-0.1 0.620 360.0 360.0 -84.7 360.0 31.5 -14.9 -4.0 63 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 64 66 A S > 0 0 12 0, 0.0 3,-1.4 0, 0.0 -2,-0.1 0.000 360.0 360.0 360.0 74.9 25.2 -14.4 -3.0 65 67 A K T 3 + 0 0 15 1,-0.3 3,-0.2 2,-0.0 13,-0.2 0.523 360.0 75.3 -81.2 -11.7 22.3 -13.0 -1.1 66 68 A T T 3 S+ 0 0 0 1,-0.2 2,-1.0 12,-0.1 -1,-0.3 0.721 85.3 72.6 -69.7 -19.6 19.3 -14.3 -3.1 67 69 A F < + 0 0 0 -3,-1.4 2,-0.4 -30,-0.1 148,-0.3 -0.491 67.3 115.8 -99.5 60.2 19.9 -17.7 -1.5 68 70 A I - 0 0 3 -2,-1.0 2,-0.8 -34,-0.4 -31,-0.2 -0.996 63.4-131.3-126.6 127.4 18.7 -17.1 2.1 69 71 A N E -e 216 0A 53 146,-2.4 148,-2.6 -2,-0.4 2,-0.8 -0.691 23.4-151.7 -75.2 105.9 15.7 -19.0 3.5 70 72 A H E -e 217 0A 36 -2,-0.8 3,-0.3 146,-0.2 2,-0.2 -0.789 22.4-179.2 -85.7 110.5 13.6 -16.2 5.0 71 73 A T > + 0 0 37 146,-2.2 3,-2.1 -2,-0.8 145,-0.0 -0.491 50.4 32.8-100.8 169.6 11.6 -17.6 7.9 72 74 A Q T 3 S- 0 0 112 1,-0.3 -1,-0.2 -2,-0.2 146,-0.1 0.667 125.6 -64.9 62.3 25.1 9.0 -16.3 10.3 73 75 A G T 3 S+ 0 0 63 -3,-0.3 -1,-0.3 1,-0.3 -2,-0.1 0.536 84.5 159.4 78.1 8.5 7.5 -13.8 7.9 74 76 A I < - 0 0 7 -3,-2.1 -1,-0.3 143,-0.2 143,-0.1 -0.486 46.3-114.2 -66.8 125.9 10.7 -11.7 7.6 75 77 A P - 0 0 20 0, 0.0 2,-0.9 0, 0.0 3,-0.1 -0.319 23.4-128.4 -60.3 143.3 10.6 -9.7 4.4 76 78 A D > - 0 0 23 1,-0.2 4,-1.9 -6,-0.1 5,-0.2 -0.825 20.8-171.8-102.5 99.3 13.2 -10.7 1.8 77 79 A F T 4 S+ 0 0 3 -2,-0.9 4,-0.4 1,-0.2 -1,-0.2 0.822 83.6 46.2 -59.0 -35.6 15.1 -7.6 0.7 78 80 A F T >4 S+ 0 0 5 -13,-0.2 3,-1.1 2,-0.2 4,-0.4 0.951 111.3 44.6 -80.5 -51.0 17.0 -9.5 -1.9 79 81 A K G >4 S+ 0 0 4 1,-0.3 3,-1.1 -14,-0.2 -2,-0.2 0.888 112.4 55.4 -63.3 -34.8 14.3 -11.6 -3.7 80 82 A Q G 3< S+ 0 0 41 -4,-1.9 -1,-0.3 1,-0.2 -2,-0.2 0.718 94.6 70.4 -66.8 -22.5 12.0 -8.5 -3.7 81 83 A S G X> S+ 0 0 0 -3,-1.1 4,-2.0 -4,-0.4 3,-0.6 0.656 81.2 134.5 -76.1 -13.4 14.7 -6.5 -5.5 82 84 A F T <4 + 0 0 11 -3,-1.1 -78,-0.1 -4,-0.4 -77,-0.1 -0.530 54.0 28.5 -80.0 148.1 14.3 -8.3 -8.8 83 85 A P T 34 S+ 0 0 78 0, 0.0 25,-0.5 0, 0.0 -1,-0.3 -0.988 130.0 43.6 -81.2 -11.9 14.1 -7.6 -11.7 84 86 A E T <4 S- 0 0 86 -3,-0.6 -2,-0.3 1,-0.2 2,-0.2 0.860 96.6-155.0 -66.7 -38.0 16.2 -4.5 -10.9 85 87 A G < - 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