==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=19-FEB-2012 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 08-NOV-10 3PJ7 . COMPND 2 MOLECULE: RED FLUORESCENT PROTEIN EQFP578; . SOURCE 2 ORGANISM_SCIENTIFIC: ENTACMAEA QUADRICOLOR; . AUTHOR S.PLETNEV,N.V.PLETNEVA,V.Z.PLETNEV . 888 8 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 32491.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 621 69.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 55 6.2 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 410 46.2 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 12 1.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 56 6.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 76 8.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 24 2.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 12 1.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 4 0 0 0 0 0 0 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 4 0 0 6 2 4 4 8 4 12 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 3 A E 0 0 150 0, 0.0 76,-0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 169.5 -27.6 29.1 -26.3 2 4 A L + 0 0 83 75,-0.2 2,-0.8 1,-0.1 75,-0.0 0.638 360.0 75.3 -69.3 -20.7 -28.2 25.8 -24.5 3 5 A I S S- 0 0 4 74,-0.1 -1,-0.1 77,-0.1 4,-0.1 -0.876 74.9-166.0-100.7 105.6 -25.9 24.2 -27.1 4 6 A S - 0 0 58 -2,-0.8 29,-0.2 2,-0.2 3,-0.1 -0.192 35.5-100.8 -85.0 178.6 -22.3 25.2 -26.3 5 7 A E S S+ 0 0 112 1,-0.2 2,-0.4 26,-0.1 29,-0.2 0.651 113.9 40.1 -77.4 -17.7 -19.2 24.9 -28.5 6 8 A N S S+ 0 0 104 25,-0.2 2,-0.3 26,-0.1 25,-0.2 -0.997 79.1 174.9-131.4 130.3 -18.1 21.7 -26.7 7 9 A M E -A 30 0A 9 23,-2.6 23,-2.2 -2,-0.4 2,-0.3 -0.951 21.8-141.0-137.9 149.6 -20.8 19.2 -25.8 8 10 A H E -Ab 29 110A 103 101,-0.5 103,-2.4 -2,-0.3 2,-0.3 -0.857 16.2-161.9-110.7 152.9 -21.1 15.7 -24.3 9 11 A M E -Ab 28 111A 2 19,-1.8 19,-2.1 -2,-0.3 2,-0.4 -0.986 9.6-169.1-129.7 146.6 -23.5 12.9 -25.3 10 12 A K E -Ab 27 112A 96 101,-3.0 103,-2.4 -2,-0.3 2,-0.4 -0.999 15.4-172.0-131.5 137.6 -24.8 9.7 -23.7 11 13 A L E -Ab 26 113A 2 15,-2.4 15,-1.9 -2,-0.4 2,-0.4 -0.949 17.0-165.0-131.9 145.7 -26.8 7.1 -25.7 12 14 A Y E -Ab 25 114A 120 101,-2.7 103,-2.2 -2,-0.4 2,-0.4 -0.997 15.4-174.2-124.5 131.6 -28.8 3.9 -25.0 13 15 A M E -Ab 24 115A 4 11,-3.0 11,-2.7 -2,-0.4 2,-0.4 -0.987 7.9-180.0-130.2 134.0 -29.7 1.6 -27.9 14 16 A E E +Ab 23 116A 70 101,-2.5 103,-2.0 -2,-0.4 2,-0.3 -0.991 21.3 144.3-128.3 141.1 -31.9 -1.5 -28.0 15 17 A G E -Ab 22 117A 9 7,-2.5 7,-2.1 -2,-0.4 2,-0.4 -0.990 40.1-136.9-163.6 171.1 -32.6 -3.6 -31.1 16 18 A T E -Ab 21 118A 1 101,-1.5 103,-2.3 -2,-0.3 2,-0.5 -0.992 13.9-167.1-134.6 130.9 -33.2 -6.8 -33.0 17 19 A V E > S-Ab 20 119A 2 3,-2.0 3,-2.7 -2,-0.4 2,-0.6 -0.986 73.9 -40.8-116.0 118.0 -31.6 -7.5 -36.4 18 20 A N T 3 S- 0 0 7 101,-3.1 516,-0.1 -2,-0.5 518,-0.1 -0.598 128.9 -27.6 62.5-115.9 -33.3 -10.5 -38.1 19 21 A D T 3 S+ 0 0 81 -2,-0.6 2,-0.6 530,-0.1 -1,-0.3 0.309 114.9 107.8-105.3 7.5 -33.7 -12.7 -35.0 20 22 A H E < -A 17 0A 51 -3,-2.7 -3,-2.0 2,-0.0 2,-0.2 -0.784 52.4-157.6 -95.8 115.7 -30.7 -11.3 -33.0 21 23 A H E +A 16 0A 89 -2,-0.6 2,-0.3 -5,-0.2 -5,-0.2 -0.594 22.8 155.0 -88.0 147.8 -31.4 -9.1 -29.9 22 24 A F E -A 15 0A 3 -7,-2.1 -7,-2.5 -2,-0.2 2,-0.3 -0.984 27.5-148.7-161.1 166.7 -28.8 -6.7 -28.6 23 25 A K E -AC 14 45A 69 22,-2.2 21,-1.8 -2,-0.3 22,-1.5 -0.992 13.5-173.1-145.1 143.7 -28.2 -3.5 -26.6 24 26 A C E -AC 13 43A 2 -11,-2.7 -11,-3.0 -2,-0.3 2,-0.3 -0.932 6.6-165.3-131.7 158.2 -25.5 -0.9 -26.8 25 27 A T E -AC 12 42A 50 17,-2.1 17,-3.3 -2,-0.3 2,-0.3 -0.947 12.5-175.4-132.9 154.4 -24.5 2.3 -24.8 26 28 A S E -AC 11 41A 4 -15,-1.9 -15,-2.4 -2,-0.3 2,-0.4 -0.994 16.4-159.8-145.5 159.3 -22.2 5.0 -25.9 27 29 A E E +AC 10 40A 115 13,-1.8 13,-3.5 -2,-0.3 2,-0.3 -0.963 26.3 158.4-127.5 145.2 -20.5 8.2 -24.6 28 30 A G E -AC 9 39A 9 -19,-2.1 -19,-1.8 -2,-0.4 2,-0.3 -0.954 25.2-147.9-150.5 179.1 -19.1 10.9 -26.9 29 31 A E E +AC 8 38A 109 9,-2.0 9,-2.9 -2,-0.3 2,-0.3 -0.988 22.3 158.3-150.6 157.7 -18.1 14.4 -27.2 30 32 A G E -AC 7 37A 9 -23,-2.2 -23,-2.6 -2,-0.3 7,-0.3 -0.966 44.4-103.3-163.7 177.3 -18.1 16.9 -30.0 31 33 A K E >> - C 0 36A 62 5,-2.9 4,-2.1 -2,-0.3 5,-1.5 -0.892 30.2-166.2-118.2 91.4 -18.0 20.4 -31.3 32 34 A P T 45S+ 0 0 0 0, 0.0 -1,-0.1 0, 0.0 -27,-0.1 0.776 86.2 40.2 -54.1 -40.8 -21.6 21.2 -32.5 33 35 A Y T 45S+ 0 0 81 1,-0.2 -28,-0.1 -29,-0.2 -27,-0.1 0.733 116.2 50.6 -85.0 -23.1 -20.9 24.3 -34.5 34 36 A E T 45S- 0 0 95 -3,-0.5 33,-0.3 -29,-0.2 -1,-0.2 0.693 108.4-128.5 -84.1 -20.8 -17.7 22.9 -36.0 35 37 A G T <5 + 0 0 0 -4,-2.1 178,-2.2 1,-0.3 2,-0.3 0.807 64.7 119.8 82.5 31.3 -19.6 19.8 -37.0 36 38 A T E < +CD 31 212A 30 -5,-1.5 -5,-2.9 176,-0.2 -1,-0.3 -0.935 28.2 157.0-130.7 149.3 -17.3 17.1 -35.5 37 39 A Q E -CD 30 211A 10 174,-1.1 174,-2.0 -2,-0.3 2,-0.4 -0.968 25.3-144.7-165.5 156.7 -17.9 14.5 -32.8 38 40 A T E -CD 29 210A 38 -9,-2.9 -9,-2.0 -2,-0.3 2,-0.4 -0.993 10.2-163.4-132.4 133.0 -16.6 11.2 -31.5 39 41 A M E -CD 28 209A 7 170,-3.0 170,-2.4 -2,-0.4 2,-0.6 -0.951 6.4-158.6-116.9 133.5 -18.6 8.3 -30.0 40 42 A K E -CD 27 208A 104 -13,-3.5 -13,-1.8 -2,-0.4 2,-0.4 -0.952 25.3-163.2-110.2 113.9 -17.1 5.5 -28.0 41 43 A I E -CD 26 207A 1 166,-3.8 166,-2.0 -2,-0.6 2,-0.5 -0.811 15.9-163.3-115.2 138.3 -19.6 2.6 -28.1 42 44 A K E -CD 25 206A 118 -17,-3.3 -17,-2.1 -2,-0.4 2,-0.8 -0.952 15.2-146.6-116.7 130.6 -20.1 -0.6 -26.1 43 45 A V E +C 24 0A 15 162,-2.6 -19,-0.2 -2,-0.5 3,-0.1 -0.867 29.6 169.9 -86.8 112.4 -22.1 -3.6 -27.2 44 46 A V E + 0 0 83 -21,-1.8 2,-0.3 -2,-0.8 -20,-0.2 0.536 57.3 28.4-107.9 -16.3 -23.4 -4.6 -23.8 45 47 A E E S+C 23 0A 104 -22,-1.5 -22,-2.2 1,-0.0 -1,-0.2 -0.979 117.9 23.1-145.3 140.9 -26.0 -7.3 -24.4 46 48 A G S S- 0 0 27 -2,-0.3 -2,-0.1 1,-0.3 -22,-0.0 0.478 91.9-169.0 86.3 5.7 -26.2 -9.8 -27.4 47 49 A G + 0 0 42 2,-0.1 -1,-0.3 -24,-0.1 2,-0.1 -0.494 56.9 40.7 -89.0 155.7 -22.4 -9.6 -28.2 48 50 A P S S- 0 0 77 0, 0.0 156,-0.1 0, 0.0 155,-0.1 0.626 88.4-137.4 -66.4 151.2 -20.7 -10.4 -30.3 49 51 A L - 0 0 6 154,-0.3 4,-0.1 2,-0.1 -2,-0.1 -0.551 4.6-139.0 -74.8 136.6 -23.2 -9.4 -33.0 50 52 A P S S+ 0 0 47 0, 0.0 2,-0.3 0, 0.0 81,-0.1 0.554 83.9 63.2 -74.1 -3.1 -23.6 -11.8 -35.8 51 53 A F S S- 0 0 1 79,-0.1 78,-0.2 80,-0.1 -2,-0.1 -0.854 100.4 -83.6-121.4 155.0 -23.7 -8.9 -38.3 52 54 A A > - 0 0 0 76,-2.4 3,-1.5 -2,-0.3 4,-0.2 -0.322 32.0-136.8 -54.0 130.2 -21.3 -6.2 -39.3 53 55 A F G > S+ 0 0 0 1,-0.3 3,-2.1 2,-0.2 4,-0.4 0.749 94.5 84.1 -67.8 -19.4 -21.4 -3.2 -36.9 54 56 A D G > S+ 0 0 6 1,-0.3 3,-1.3 2,-0.2 4,-0.4 0.804 77.9 63.4 -48.1 -38.5 -21.4 -1.0 -39.9 55 57 A I G < S+ 0 0 2 -3,-1.5 -1,-0.3 1,-0.3 -2,-0.2 0.685 105.0 48.1 -68.0 -16.1 -25.2 -1.3 -40.3 56 58 A L G X S+ 0 0 0 -3,-2.1 3,-2.0 -4,-0.2 4,-0.4 0.540 83.4 97.4 -94.1 -2.9 -25.5 0.4 -37.0 57 59 A A G X S+ 0 0 4 -3,-1.3 3,-1.1 -4,-0.4 -2,-0.1 0.855 81.0 50.0 -58.1 -41.2 -23.1 3.3 -37.6 58 60 A T G 3 S+ 0 0 30 -4,-0.4 -1,-0.3 1,-0.2 -2,-0.1 0.438 99.9 66.9 -82.8 3.7 -25.7 5.9 -38.6 59 61 A S G < 0 0 1 -3,-2.0 -1,-0.2 1,-0.1 -2,-0.2 0.534 360.0 360.0-101.1 -7.6 -27.9 5.3 -35.6 60 62 A F < 0 0 11 -3,-1.1 -2,-0.2 -4,-0.4 -3,-0.1 0.767 360.0 360.0 -86.6 360.0 -25.4 6.6 -33.1 61 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 62 66 A S > 0 0 12 0, 0.0 3,-1.2 0, 0.0 -2,-0.1 0.000 360.0 360.0 360.0 64.7 -26.4 12.7 -33.8 63 67 A K T 3 + 0 0 3 1,-0.3 3,-0.4 2,-0.1 13,-0.2 0.582 360.0 76.0 -79.2 -15.1 -27.8 15.7 -35.5 64 68 A T T 3 S+ 0 0 0 1,-0.2 2,-0.6 12,-0.2 -1,-0.3 0.717 85.4 70.7 -57.7 -22.5 -26.9 18.5 -33.2 65 69 A F < + 0 0 0 -3,-1.2 2,-0.5 -30,-0.1 148,-0.3 -0.364 65.9 118.4-102.6 53.2 -23.4 18.3 -34.5 66 70 A I - 0 0 0 -2,-0.6 2,-1.0 -3,-0.4 -31,-0.2 -0.976 61.7-132.9-113.4 133.2 -23.7 19.7 -38.1 67 71 A N E -e 214 0A 52 146,-2.6 148,-2.5 -2,-0.5 2,-0.8 -0.686 23.2-152.5 -80.9 103.8 -21.8 22.9 -39.0 68 72 A H E -e 215 0A 29 -2,-1.0 3,-0.4 146,-0.2 148,-0.1 -0.692 19.3-174.8 -83.0 114.2 -24.5 24.9 -40.8 69 73 A T > + 0 0 40 146,-1.8 3,-2.2 -2,-0.8 -1,-0.0 -0.356 53.9 33.9 -98.6 176.0 -22.8 27.2 -43.2 70 74 A Q T 3 S- 0 0 104 1,-0.3 -1,-0.2 -2,-0.1 146,-0.1 0.676 125.6 -67.8 59.4 19.0 -24.1 30.0 -45.5 71 75 A G T 3 S+ 0 0 62 -3,-0.4 -1,-0.3 1,-0.2 -2,-0.1 0.670 80.7 161.8 78.5 15.0 -26.7 31.1 -43.0 72 76 A I < - 0 0 6 -3,-2.2 -1,-0.2 143,-0.1 143,-0.1 -0.580 46.0-115.9 -68.4 120.3 -28.9 27.9 -43.4 73 77 A P - 0 0 20 0, 0.0 2,-0.9 0, 0.0 -1,-0.1 -0.332 24.4-125.3 -61.4 152.8 -31.1 27.9 -40.2 74 78 A D > - 0 0 20 1,-0.2 4,-2.4 -6,-0.1 5,-0.2 -0.819 23.4-173.2-111.9 100.5 -30.3 25.0 -38.0 75 79 A F H > S+ 0 0 3 -2,-0.9 4,-0.6 1,-0.2 -1,-0.2 0.830 85.2 48.9 -55.3 -37.7 -33.5 23.0 -37.2 76 80 A F H >4 S+ 0 0 0 2,-0.2 3,-0.7 -13,-0.2 4,-0.3 0.946 112.6 42.3 -77.4 -53.4 -31.7 20.8 -34.7 77 81 A K H >4 S+ 0 0 8 1,-0.2 3,-1.3 -14,-0.2 -2,-0.2 0.903 112.9 55.4 -61.2 -39.0 -29.9 23.4 -32.6 78 82 A Q H 3< S+ 0 0 46 -4,-2.4 -1,-0.2 1,-0.2 -2,-0.2 0.725 92.2 74.8 -60.7 -24.4 -33.0 25.6 -32.6 79 83 A S T - G 0 168A 5 4,-2.8 3,-1.8 -2,-0.5 76,-0.3 -0.652 24.1-121.5 -97.3 150.9 -29.7 -2.3 -48.7 93 97 A E T 3 S+ 0 0 71 74,-2.8 75,-0.1 1,-0.3 -1,-0.1 0.701 109.1 59.4 -67.7 -19.9 -29.7 -3.4 -52.2 94 98 A D T 3 S- 0 0 48 73,-0.3 -1,-0.3 2,-0.1 74,-0.1 0.184 126.9 -90.6 -96.6 15.2 -29.5 -7.1 -51.3 95 99 A G S < S+ 0 0 18 -3,-1.8 -2,-0.1 1,-0.4 2,-0.1 0.245 82.2 134.2 107.6 -2.7 -32.8 -7.0 -49.4 96 100 A G - 0 0 0 -5,-0.1 -4,-2.8 23,-0.1 2,-0.4 -0.465 34.0-166.6 -70.8 146.7 -31.7 -6.1 -45.8 97 101 A V E -FH 91 120A 8 23,-2.0 23,-2.5 -6,-0.2 2,-0.4 -0.999 8.4-171.1-137.8 134.6 -33.7 -3.4 -44.2 98 102 A L E -FH 90 119A 1 -8,-2.4 -8,-1.9 -2,-0.4 2,-0.3 -0.954 4.1-172.9-127.6 117.4 -32.8 -1.5 -41.0 99 103 A T E -FH 89 118A 0 19,-2.8 19,-2.8 -2,-0.4 2,-0.3 -0.767 5.7-179.6-107.6 149.8 -35.3 0.8 -39.4 100 104 A A E -FH 88 117A 1 -12,-1.7 -12,-2.2 -2,-0.3 2,-0.4 -0.993 12.9-168.4-143.8 142.3 -34.5 3.1 -36.4 101 105 A T E -FH 87 116A 5 15,-2.1 15,-2.2 -2,-0.3 2,-0.4 -0.987 18.3-172.1-135.0 145.2 -36.4 5.6 -34.3 102 106 A Q E -FH 86 115A 7 -16,-2.3 -16,-1.6 -2,-0.4 2,-0.4 -0.990 20.1-149.9-145.2 140.0 -34.6 7.9 -31.9 103 107 A D E -FH 85 114A 40 11,-2.6 11,-2.1 -2,-0.4 2,-0.4 -0.916 10.9-163.8-110.5 139.2 -35.2 10.4 -29.2 104 108 A T E +FH 84 113A 0 -20,-2.4 -20,-1.4 -2,-0.4 2,-0.3 -0.986 18.7 163.9-119.6 117.2 -32.9 13.4 -28.6 105 109 A S E - 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