==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=14-JUN-2013 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER BIOTIN-BINDING PROTEIN 10-MAY-11 3RY1 . COMPND 2 MOLECULE: STREPTAVIDIN; . SOURCE 2 ORGANISM_SCIENTIFIC: STREPTOMYCES AVIDINII; . AUTHOR R.E.STENKAMP,Z.WANG,I.LE TRONG,P.S.STAYTON,T.P.LYBRAND . 490 4 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 20816.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 327 66.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 240 49.0 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 1.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 4 0.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 51 10.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 31 6.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 6 0 0 5 3 6 2 1 7 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 15 A A 0 0 124 0, 0.0 83,-0.0 0, 0.0 3,-0.0 0.000 360.0 360.0 360.0 166.2 10.8 28.8 12.9 2 16 A G - 0 0 30 1,-0.1 81,-0.1 3,-0.0 86,-0.0 0.155 360.0 -87.4 95.3 161.6 14.1 26.8 12.3 3 17 A I S S+ 0 0 14 79,-0.1 14,-0.2 2,-0.0 -1,-0.1 0.885 97.3 90.6 -71.8 -41.3 15.6 23.8 14.0 4 18 A T S S+ 0 0 69 15,-0.1 2,-0.3 12,-0.1 14,-0.2 -0.386 70.4 65.5 -61.3 133.2 17.5 25.6 16.8 5 19 A G E S-A 17 0A 22 12,-2.5 12,-2.6 -2,-0.1 2,-0.5 -0.958 88.9 -54.3 151.0-171.9 15.3 25.9 19.8 6 20 A T E +A 16 0A 53 -2,-0.3 2,-0.3 10,-0.2 10,-0.2 -0.935 53.2 180.0-107.3 122.6 13.6 24.1 22.6 7 21 A W E -A 15 0A 0 8,-3.0 8,-3.0 -2,-0.5 2,-0.4 -0.824 14.0-154.5-120.9 159.2 11.3 21.3 21.5 8 22 A Y E -AB 14 117A 54 109,-2.7 109,-2.2 -2,-0.3 6,-0.2 -0.995 11.0-139.5-136.0 138.5 9.0 18.8 23.3 9 23 A N > - 0 0 4 4,-1.6 3,-1.9 -2,-0.4 107,-0.2 -0.288 43.1 -85.8 -85.0-177.6 7.8 15.4 22.2 10 24 A Q T 3 S+ 0 0 75 105,-0.4 106,-0.1 1,-0.3 -1,-0.1 0.632 129.8 48.8 -68.4 -14.3 4.3 14.0 22.7 11 25 A L T 3 S- 0 0 61 2,-0.1 -1,-0.3 111,-0.0 109,-0.1 0.395 122.6-101.6-101.0 -0.1 5.3 12.7 26.1 12 26 A G < + 0 0 10 -3,-1.9 -2,-0.1 1,-0.3 2,-0.0 0.525 65.1 155.7 91.3 7.4 6.8 16.0 27.3 13 27 A S - 0 0 2 1,-0.1 -4,-1.6 18,-0.0 2,-0.5 -0.403 40.5-128.0 -61.7 148.5 10.5 15.1 26.8 14 28 A T E -AC 8 30A 19 16,-2.4 16,-2.4 -6,-0.2 2,-0.6 -0.912 22.7-172.1-111.7 122.0 12.6 18.3 26.3 15 29 A F E -AC 7 29A 1 -8,-3.0 -8,-3.0 -2,-0.5 2,-0.6 -0.952 1.2-173.7-117.7 108.5 15.0 18.5 23.3 16 30 A I E +AC 6 28A 44 12,-2.9 12,-2.0 -2,-0.6 2,-0.4 -0.931 20.9 169.8-107.9 111.2 17.2 21.6 23.5 17 31 A V E -AC 5 27A 1 -12,-2.6 -12,-2.5 -2,-0.6 2,-0.4 -0.951 29.6-158.8-134.2 147.9 19.1 21.8 20.2 18 32 A T E - C 0 26A 74 8,-2.3 8,-1.8 -2,-0.4 2,-0.7 -0.998 14.1-148.4-122.8 127.5 21.4 24.1 18.2 19 33 A A E - C 0 25A 22 -2,-0.4 6,-0.2 6,-0.2 2,-0.2 -0.872 21.2-150.0 -94.9 114.0 21.8 23.7 14.5 20 34 A G > - 0 0 21 4,-3.0 3,-2.0 -2,-0.7 26,-0.1 -0.499 24.7-116.0 -84.3 157.3 25.3 24.8 13.6 21 35 A A T 3 S+ 0 0 102 1,-0.3 -1,-0.1 -2,-0.2 27,-0.0 0.725 115.1 63.1 -61.3 -23.0 26.3 26.3 10.3 22 36 A D T 3 S- 0 0 109 2,-0.1 -1,-0.3 1,-0.0 26,-0.1 0.263 124.2 -98.5 -83.9 6.1 28.5 23.2 9.8 23 37 A G S < S+ 0 0 1 -3,-2.0 23,-2.1 1,-0.3 2,-0.2 0.456 79.8 139.1 89.2 2.6 25.5 20.7 9.8 24 38 A A E - D 0 45A 19 21,-0.2 -4,-3.0 19,-0.0 2,-0.4 -0.543 41.1-156.5 -82.6 147.2 26.0 19.8 13.4 25 39 A L E +CD 19 44A 1 19,-2.4 19,-2.2 -6,-0.2 2,-0.3 -0.985 22.5 167.2-111.7 132.2 23.3 19.3 16.0 26 40 A T E +CD 18 43A 70 -8,-1.8 -8,-2.3 -2,-0.4 2,-0.3 -0.991 19.3 85.4-141.1 151.6 24.3 19.7 19.6 27 41 A G E -CD 17 42A 23 15,-1.4 15,-2.2 -2,-0.3 2,-0.3 -0.991 62.6 -60.4 160.5-150.0 22.4 20.1 22.8 28 42 A T E -CD 16 41A 38 -12,-2.0 -12,-2.9 -2,-0.3 2,-0.4 -0.947 27.2-155.8-136.3 154.4 20.7 18.3 25.6 29 43 A Y E -CD 15 40A 3 11,-2.5 11,-2.3 -2,-0.3 2,-0.5 -0.995 3.2-169.5-132.3 133.7 17.8 15.9 26.0 30 44 A E E -C 14 0A 83 -16,-2.4 -16,-2.4 -2,-0.4 2,-0.3 -0.990 20.5-140.9-128.1 117.0 15.6 15.3 29.1 31 45 A S - 0 0 27 7,-0.5 7,-0.2 -2,-0.5 5,-0.1 -0.630 14.4-157.9 -85.4 137.5 13.3 12.3 28.9 32 46 A A S S+ 0 0 53 -2,-0.3 2,-0.3 5,-0.1 -1,-0.1 0.586 82.0 36.7 -79.2 -13.8 9.8 12.2 30.3 33 47 A V S S+ 0 0 44 2,-0.0 -2,-0.1 444,-0.0 442,-0.0 -0.905 91.4 26.6-136.6 162.4 10.0 8.4 30.5 34 48 A G S S- 0 0 49 -2,-0.3 2,-1.9 1,-0.1 -3,-0.0 -0.193 101.7 -31.2 86.9-173.0 12.4 5.5 31.2 35 49 A N S S+ 0 0 91 37,-0.1 2,-0.3 2,-0.0 37,-0.1 -0.583 98.1 116.2 -86.4 75.0 15.5 5.1 33.3 36 50 A A - 0 0 20 -2,-1.9 2,-0.3 -5,-0.1 3,-0.1 -0.993 40.4-174.4-150.4 139.7 16.6 8.6 32.9 37 51 A E - 0 0 87 -2,-0.3 32,-0.2 2,-0.1 33,-0.2 -0.980 55.3 -21.8-142.6 130.3 17.2 11.6 35.1 38 52 A S S S- 0 0 43 -2,-0.3 -7,-0.5 -7,-0.2 2,-0.2 -0.193 102.8 -21.6 63.8-166.3 18.1 15.2 34.2 39 53 A R - 0 0 138 -9,-0.2 2,-0.3 -3,-0.1 -9,-0.2 -0.518 53.1-163.3 -81.1 147.7 19.7 16.2 31.0 40 54 A Y E -D 29 0A 2 -11,-2.3 -11,-2.5 -2,-0.2 2,-0.2 -0.931 29.0-104.9-121.2 148.4 21.8 14.0 28.7 41 55 A V E -D 28 0A 69 25,-0.4 23,-0.5 -2,-0.3 2,-0.3 -0.482 36.5-169.5 -66.8 140.5 24.1 15.1 25.9 42 56 A L E +DE 27 63A 2 -15,-2.2 -15,-1.4 -2,-0.2 2,-0.3 -0.966 9.4 178.3-126.4 154.9 23.0 14.8 22.3 43 57 A T E +DE 26 62A 19 19,-1.5 19,-2.5 -2,-0.3 2,-0.3 -0.988 17.5 135.7-144.8 149.3 24.9 15.2 19.0 44 58 A G E -DE 25 61A 0 -19,-2.2 -19,-2.4 -2,-0.3 2,-0.3 -0.892 38.4-105.9-167.3-164.6 23.6 14.7 15.5 45 59 A R E -DE 24 60A 24 15,-2.0 15,-2.5 -21,-0.3 2,-0.3 -0.971 20.1-170.5-140.9 153.9 23.6 16.1 12.0 46 60 A Y E - E 0 59A 32 -23,-2.1 2,-0.9 -2,-0.3 13,-0.2 -0.973 38.4 -97.7-143.5 157.3 21.1 17.9 9.7 47 61 A D - 0 0 2 11,-2.5 -24,-0.1 -2,-0.3 152,-0.0 -0.674 32.9-168.0 -76.1 106.5 21.0 18.8 6.0 48 62 A S S S+ 0 0 52 -2,-0.9 -1,-0.2 1,-0.3 -25,-0.1 0.439 82.6 43.3 -79.3 -1.2 22.1 22.5 6.1 49 63 A A S S- 0 0 48 -26,-0.0 -1,-0.3 0, 0.0 148,-0.1 -0.525 83.7-174.4-139.8 69.2 21.0 23.1 2.4 50 64 A P - 0 0 49 0, 0.0 7,-0.1 0, 0.0 6,-0.0 -0.090 35.3 -82.5 -63.7 164.8 17.6 21.4 2.1 51 65 A A - 0 0 26 5,-0.4 3,-0.3 1,-0.1 173,-0.0 -0.234 28.9-141.1 -59.6 156.4 15.6 21.1 -1.1 52 66 A T S S+ 0 0 149 1,-0.2 -1,-0.1 2,-0.1 0, 0.0 0.093 79.6 92.6-109.2 21.8 13.6 24.1 -2.2 53 67 A D S S- 0 0 113 2,-0.0 -1,-0.2 172,-0.0 172,-0.1 0.267 113.0 -83.7-101.7 7.7 10.6 22.1 -3.5 54 68 A G S S+ 0 0 62 1,-0.3 172,-0.3 -3,-0.3 2,-0.1 0.386 86.9 136.9 99.7 -2.2 8.5 22.0 -0.3 55 69 A S - 0 0 25 170,-0.1 -1,-0.3 1,-0.1 170,-0.3 -0.415 54.4-109.5 -74.1 154.3 10.3 19.0 1.3 56 70 A G - 0 0 8 168,-2.2 2,-0.6 166,-0.1 -5,-0.4 -0.076 34.4 -97.2 -69.6 176.6 11.3 18.9 5.0 57 71 A T E - F 0 82A 17 25,-2.4 25,-2.5 -7,-0.1 -1,-0.1 -0.911 36.3-132.9-105.9 113.8 14.8 19.0 6.3 58 72 A A E + F 0 81A 0 -2,-0.6 -11,-2.5 23,-0.2 2,-0.3 -0.338 39.0 149.0 -69.2 150.2 16.4 15.7 7.1 59 73 A L E -EF 46 80A 1 21,-2.3 21,-3.0 -13,-0.2 2,-0.3 -0.992 22.0-170.4-171.9 161.4 18.2 15.2 10.4 60 74 A G E -EF 45 79A 0 -15,-2.5 -15,-2.0 -2,-0.3 2,-0.3 -0.993 3.8-175.5-158.0 157.5 19.1 12.8 13.1 61 75 A W E -EF 44 78A 0 17,-2.1 17,-2.6 -2,-0.3 2,-0.4 -0.987 13.2-138.1-153.8 164.3 20.6 12.7 16.5 62 76 A T E -EF 43 77A 0 -19,-2.5 -19,-1.5 -2,-0.3 2,-0.4 -0.970 7.7-170.9-125.4 141.3 21.8 10.3 19.3 63 77 A V E -EF 42 76A 0 13,-2.5 13,-2.5 -2,-0.4 2,-0.5 -0.996 11.1-156.5-123.1 122.7 21.5 10.2 23.1 64 78 A A E - F 0 75A 3 -23,-0.5 2,-1.9 -2,-0.4 11,-0.3 -0.888 26.5-127.0 -88.6 130.9 23.4 7.6 25.0 65 79 A W E S+ 0 0 16 9,-2.8 7,-2.6 -2,-0.5 2,-0.4 -0.193 75.4 105.2 -82.9 49.2 21.5 7.2 28.3 66 80 A K E + F 0 71A 57 -2,-1.9 -25,-0.4 5,-0.2 5,-0.2 -0.997 35.4 148.4-122.5 129.0 24.5 7.8 30.5 67 81 A N - 0 0 25 3,-2.4 -27,-0.1 -2,-0.4 -29,-0.1 -0.640 65.9 -77.6-135.4-160.9 25.1 11.1 32.4 68 82 A N S S+ 0 0 160 -2,-0.2 3,-0.1 1,-0.2 -28,-0.1 0.514 128.1 41.5 -81.0 -3.4 26.7 11.9 35.7 69 83 A Y S S+ 0 0 152 1,-0.3 2,-0.3 -32,-0.2 -1,-0.2 0.726 120.7 8.0-109.5 -36.3 23.7 10.6 37.6 70 84 A R - 0 0 98 -33,-0.2 -3,-2.4 -35,-0.0 2,-0.3 -0.972 49.1-154.6-149.9 157.9 22.5 7.4 35.9 71 85 A N E +F 66 0A 41 -2,-0.3 -5,-0.2 -5,-0.2 -35,-0.1 -0.921 10.3 177.1-142.5 112.0 23.4 4.9 33.2 72 86 A A E - 0 0 25 -7,-2.6 -6,-0.1 -2,-0.3 3,-0.1 0.310 43.6-127.2 -93.2 4.8 20.8 2.8 31.4 73 87 A H E S+ 0 0 58 -8,-0.3 26,-2.6 1,-0.2 2,-0.3 0.918 74.5 103.0 51.1 50.8 23.3 1.1 29.1 74 88 A S E - G 0 98A 10 24,-0.3 -9,-2.8 108,-0.1 2,-0.3 -0.966 54.5-152.7-155.4 161.0 21.3 2.2 26.1 75 89 A A E -FG 64 97A 0 22,-2.2 22,-2.5 -2,-0.3 2,-0.4 -0.990 5.8-152.5-146.4 137.4 21.2 4.7 23.3 76 90 A T E -FG 63 96A 7 -13,-2.5 -13,-2.5 -2,-0.3 2,-0.5 -0.922 6.7-165.0-104.2 136.9 18.3 6.2 21.2 77 91 A T E -FG 62 95A 0 18,-2.4 18,-2.1 -2,-0.4 2,-0.5 -0.991 5.3-158.6-115.5 130.6 18.8 7.5 17.7 78 92 A W E -FG 61 94A 6 -17,-2.6 -17,-2.1 -2,-0.5 2,-0.4 -0.918 8.1-174.1-105.6 130.2 16.1 9.7 16.2 79 93 A S E +FG 60 93A 0 14,-2.8 14,-2.6 -2,-0.5 2,-0.3 -0.986 32.6 102.8-121.6 128.4 15.9 9.9 12.4 80 94 A G E -FG 59 92A 1 -21,-3.0 -21,-2.3 -2,-0.4 2,-0.3 -0.882 55.8 -88.7-171.9-157.6 13.5 12.4 10.9 81 95 A Q E -FG 58 91A 5 10,-2.1 10,-2.4 -2,-0.3 2,-0.4 -0.999 23.3-125.6-147.1 143.0 12.7 15.7 9.2 82 96 A Y E -FG 57 90A 25 -25,-2.5 -25,-2.4 -2,-0.3 2,-0.5 -0.754 25.4-160.2 -82.9 133.9 11.9 19.2 10.3 83 97 A V E - 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