==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=11-SEP-2011 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 12-JUL-11 3SVR . COMPND 2 MOLECULE: MKATE S158A/S143C; . SOURCE 2 ORGANISM_SCIENTIFIC: ARTIFICIAL GENE; . AUTHOR Q.WANG,L.BYRNES,H.SONDERMANN . 899 9 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 32661.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 656 73.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 52 5.8 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 407 45.3 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 11 1.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 1 0.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 89 9.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 78 8.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 25 2.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 12 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 4 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 4 0 1 7 0 4 4 8 4 12 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 4 A L 0 0 135 0, 0.0 2,-1.0 0, 0.0 75,-0.0 0.000 360.0 360.0 360.0 -46.4 10.6 -14.2 25.5 2 5 A I - 0 0 12 74,-0.1 4,-0.1 77,-0.1 74,-0.0 -0.781 360.0-168.5 -93.6 101.0 12.5 -16.1 28.2 3 6 A T - 0 0 77 -2,-1.0 29,-0.2 2,-0.2 3,-0.1 -0.137 38.0 -94.2 -84.1-179.5 11.7 -19.7 27.6 4 7 A E S S+ 0 0 122 1,-0.2 2,-0.4 26,-0.1 28,-0.3 0.791 116.6 34.2 -66.0 -31.9 12.3 -22.9 29.7 5 8 A N S S+ 0 0 92 25,-0.2 2,-0.3 26,-0.1 -2,-0.2 -0.997 76.1 177.0-125.5 127.2 15.5 -23.5 27.8 6 9 A M E -A 29 0A 7 23,-2.2 23,-2.3 -2,-0.4 2,-0.3 -0.965 18.4-141.9-131.0 148.0 17.7 -20.7 26.6 7 10 A H E -A 28 0A 111 101,-0.5 103,-2.3 -2,-0.3 2,-0.4 -0.750 14.7-154.2-103.0 156.0 21.1 -20.5 24.8 8 11 A M E -Ab 27 110A 2 19,-2.4 19,-2.0 -2,-0.3 2,-0.4 -0.979 10.1-170.4-129.9 143.5 23.8 -17.9 25.4 9 12 A K E -Ab 26 111A 121 101,-2.3 103,-2.9 -2,-0.4 2,-0.4 -0.995 16.9-171.8-126.7 139.1 26.7 -16.4 23.3 10 13 A L E -Ab 25 112A 9 15,-2.6 15,-1.9 -2,-0.4 2,-0.4 -0.972 18.5-168.7-136.6 145.2 29.4 -14.2 24.9 11 14 A Y E -Ab 24 113A 71 101,-2.2 103,-3.0 -2,-0.4 2,-0.4 -0.999 15.2-177.2-128.9 135.9 32.3 -12.0 23.9 12 15 A M E +Ab 23 114A 0 11,-2.6 11,-2.7 -2,-0.4 2,-0.3 -0.994 6.1 170.5-138.7 138.1 34.8 -10.7 26.5 13 16 A E E +Ab 22 115A 60 101,-1.8 103,-2.1 -2,-0.4 2,-0.3 -0.997 21.5 98.3-147.2 142.1 37.8 -8.4 26.2 14 17 A G E -Ab 21 116A 5 7,-2.1 7,-2.1 -2,-0.3 2,-0.3 -0.987 50.6 -87.2 174.9-158.2 40.0 -6.7 28.7 15 18 A T E -Ab 20 117A 0 101,-1.5 103,-3.0 -2,-0.3 2,-0.4 -0.994 12.7-160.7-142.2 142.2 43.3 -6.4 30.6 16 19 A V E > S-Ab 19 118A 2 3,-1.9 3,-2.1 -2,-0.3 2,-0.9 -0.979 76.7 -42.1-122.5 120.3 44.5 -7.7 34.0 17 20 A N T 3 S- 0 0 6 101,-3.0 523,-0.1 -2,-0.4 525,-0.1 -0.540 129.2 -24.5 62.5-102.3 47.5 -5.8 35.3 18 21 A N T 3 S+ 0 0 87 -2,-0.9 2,-0.6 537,-0.1 -1,-0.3 0.188 115.7 103.1-121.5 17.0 49.5 -5.4 32.0 19 22 A H E < -A 16 0A 51 -3,-2.1 -3,-1.9 2,-0.0 2,-0.2 -0.882 53.6-156.0-116.0 114.7 48.1 -8.4 30.2 20 23 A H E +A 15 0A 85 -2,-0.6 2,-0.3 -5,-0.2 -5,-0.2 -0.475 23.3 158.8 -86.7 151.7 45.5 -8.1 27.4 21 24 A F E -A 14 0A 2 -7,-2.1 -7,-2.1 -2,-0.2 2,-0.3 -0.987 25.4-150.7-163.2 164.8 43.1 -11.0 26.5 22 25 A K E -AC 13 44A 47 22,-2.0 21,-2.0 -2,-0.3 22,-1.5 -0.993 12.0-173.4-145.7 148.0 39.9 -12.0 24.9 23 26 A C E -AC 12 42A 0 -11,-2.7 -11,-2.6 -2,-0.3 2,-0.3 -0.937 7.0-164.1-135.3 158.9 37.4 -14.8 25.4 24 27 A T E +AC 11 41A 49 17,-2.1 17,-3.2 -2,-0.3 2,-0.3 -0.976 14.4 178.6-135.7 152.4 34.3 -16.2 23.8 25 28 A S E -AC 10 40A 10 -15,-1.9 -15,-2.6 -2,-0.3 2,-0.3 -0.980 15.2-167.7-147.5 162.6 31.8 -18.6 25.1 26 29 A E E +AC 9 39A 91 13,-1.7 13,-3.1 -2,-0.3 2,-0.3 -0.975 24.3 137.8-142.3 152.3 28.6 -20.4 24.4 27 30 A G E -AC 8 38A 0 -19,-2.0 -19,-2.4 -2,-0.3 2,-0.3 -0.923 31.5-132.8-167.0-167.2 26.3 -22.3 26.7 28 31 A E E +AC 7 37A 65 9,-1.4 9,-2.3 -21,-0.3 2,-0.3 -0.976 22.2 165.3-158.8 159.3 22.7 -23.0 27.6 29 32 A G E -AC 6 36A 9 -23,-2.3 -23,-2.2 -2,-0.3 7,-0.3 -0.942 42.4 -94.9-162.2-174.8 20.6 -23.2 30.8 30 33 A K E >>> - C 0 35A 79 5,-2.6 4,-1.5 -2,-0.3 5,-1.3 -0.857 30.3-165.3-119.9 93.5 17.2 -23.3 32.4 31 34 A P T 345S+ 0 0 0 0, 0.0 34,-0.3 0, 0.0 -1,-0.1 0.767 86.8 45.7 -52.3 -38.3 16.3 -19.7 33.3 32 35 A Y T 345S+ 0 0 115 -28,-0.3 -28,-0.1 1,-0.2 -27,-0.1 0.709 114.7 46.7 -83.5 -21.2 13.4 -20.6 35.7 33 36 A E T <45S- 0 0 94 -3,-1.2 33,-0.3 -29,-0.2 -1,-0.2 0.563 109.0-125.8 -91.3 -11.7 15.3 -23.4 37.4 34 37 A G T <5 + 0 0 0 -4,-1.5 177,-2.4 1,-0.3 2,-0.3 0.800 65.5 125.0 75.8 31.0 18.3 -21.1 37.8 35 38 A T E < +CD 30 210A 35 -5,-1.3 -5,-2.6 175,-0.2 2,-0.3 -0.927 26.7 158.5-124.5 146.4 20.9 -23.4 36.2 36 39 A Q E -CD 29 209A 12 173,-1.6 173,-2.0 -2,-0.3 2,-0.4 -0.982 23.4-149.3-160.4 157.3 23.3 -22.8 33.3 37 40 A T E -CD 28 208A 26 -9,-2.3 -9,-1.4 -2,-0.3 2,-0.4 -0.989 9.6-167.8-131.0 138.7 26.5 -24.0 31.7 38 41 A M E -CD 27 207A 6 169,-2.5 169,-2.5 -2,-0.4 2,-0.6 -0.997 10.3-156.3-124.5 134.0 29.1 -22.1 29.7 39 42 A R E -CD 26 206A 51 -13,-3.1 -13,-1.7 -2,-0.4 2,-0.4 -0.948 26.5-162.4-105.5 121.1 31.9 -23.5 27.6 40 43 A I E -CD 25 205A 1 165,-3.5 165,-2.2 -2,-0.6 2,-0.4 -0.861 15.4-164.4-118.3 140.8 34.6 -20.8 27.4 41 44 A K E -CD 24 204A 122 -17,-3.2 -17,-2.1 -2,-0.4 2,-0.8 -0.981 17.5-143.9-122.3 127.9 37.6 -20.2 25.2 42 45 A V E +C 23 0A 16 161,-2.6 -19,-0.3 -2,-0.4 4,-0.1 -0.822 28.9 174.1 -83.2 111.9 40.5 -17.9 25.8 43 46 A V E + 0 0 78 -21,-2.0 2,-0.3 -2,-0.8 -20,-0.2 0.514 58.4 28.5-105.9 -9.7 41.0 -16.8 22.2 44 47 A E E S+C 22 0A 106 -22,-1.5 -22,-2.0 1,-0.0 -1,-0.2 -0.976 118.7 19.2-149.7 140.5 43.7 -14.0 22.5 45 48 A G S S- 0 0 31 -2,-0.3 -2,-0.1 1,-0.3 -3,-0.0 0.544 90.5-175.3 78.6 8.5 46.4 -13.6 25.1 46 49 A G + 0 0 39 -4,-0.1 -1,-0.3 -24,-0.1 2,-0.1 -0.597 55.7 49.5 -87.6 152.0 46.3 -17.2 26.2 47 50 A P S S- 0 0 78 0, 0.0 155,-0.1 0, 0.0 154,-0.1 0.543 88.1-140.6 -72.4 146.0 47.6 -18.8 28.3 48 51 A L - 0 0 4 153,-0.4 4,-0.1 -2,-0.1 -2,-0.1 -0.513 5.8-141.8 -75.7 141.2 46.7 -16.2 30.9 49 52 A P S S+ 0 0 46 0, 0.0 2,-0.3 0, 0.0 81,-0.1 0.365 83.5 65.4 -83.5 3.9 49.4 -15.4 33.5 50 53 A F S S- 0 0 0 79,-0.1 78,-0.2 80,-0.1 -2,-0.1 -0.903 100.6 -85.0-124.5 154.9 46.6 -15.1 36.2 51 54 A A > - 0 0 0 76,-1.9 3,-1.6 -2,-0.3 4,-0.2 -0.348 30.4-136.9 -56.9 129.2 44.2 -17.6 37.7 52 55 A F G > S+ 0 0 0 1,-0.3 3,-2.8 2,-0.1 4,-0.5 0.807 93.8 83.1 -59.3 -25.8 41.1 -18.0 35.5 53 56 A D G > S+ 0 0 7 1,-0.3 3,-1.4 2,-0.2 4,-0.4 0.777 78.9 64.0 -47.8 -36.0 39.0 -17.9 38.7 54 57 A I G < S+ 0 0 2 -3,-1.6 -1,-0.3 1,-0.3 -2,-0.1 0.569 103.3 49.0 -72.5 -5.5 39.1 -14.1 38.8 55 58 A L G X S+ 0 0 0 -3,-2.8 3,-1.8 -4,-0.2 4,-0.4 0.495 84.2 96.4-102.5 -8.7 37.1 -14.0 35.6 56 59 A A G X S+ 0 0 5 -3,-1.4 3,-1.1 -4,-0.5 -2,-0.1 0.836 80.1 50.1 -55.6 -43.8 34.4 -16.5 36.7 57 60 A T G 3 S+ 0 0 32 -4,-0.4 -1,-0.3 1,-0.2 -2,-0.1 0.454 100.4 67.8 -77.1 -0.5 31.7 -14.1 37.8 58 61 A S G < 0 0 1 -3,-1.8 -1,-0.2 1,-0.2 -2,-0.2 0.599 360.0 360.0 -95.6 -11.0 32.0 -12.1 34.6 59 62 A F < 0 0 11 -3,-1.1 -1,-0.2 -4,-0.4 -2,-0.1 0.514 360.0 360.0 -89.6 360.0 30.5 -14.9 32.3 60 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 61 66 A S > 0 0 14 0, 0.0 3,-1.5 0, 0.0 -2,-0.1 0.000 360.0 360.0 360.0 71.0 24.4 -14.2 33.6 62 67 A K T 3 + 0 0 5 1,-0.3 3,-0.3 2,-0.1 13,-0.2 0.513 360.0 75.7 -79.3 -9.1 21.5 -12.9 35.7 63 68 A T T 3 S+ 0 0 0 1,-0.2 2,-0.9 12,-0.1 -1,-0.3 0.739 85.9 68.9 -69.8 -24.0 18.7 -14.2 33.5 64 69 A F S < S+ 0 0 0 -3,-1.5 2,-0.4 -30,-0.1 147,-0.3 -0.441 71.3 116.1 -96.9 59.0 19.3 -17.6 34.9 65 70 A I - 0 0 0 -2,-0.9 2,-1.1 -3,-0.3 -31,-0.2 -0.997 65.9-128.6-125.1 128.6 18.2 -17.1 38.5 66 71 A N E -e 212 0A 51 145,-2.5 147,-2.3 -2,-0.4 2,-1.2 -0.628 22.5-155.8 -75.4 99.8 15.3 -19.0 39.9 67 72 A H E -e 213 0A 27 -2,-1.1 3,-0.4 145,-0.2 147,-0.1 -0.681 21.1-176.8 -82.5 98.5 13.1 -16.2 41.4 68 73 A T > + 0 0 37 145,-1.3 3,-1.9 -2,-1.2 144,-0.0 -0.329 52.4 35.7 -86.5 170.4 11.1 -18.0 44.1 69 74 A Q T 3 S- 0 0 115 1,-0.3 -1,-0.2 -2,-0.1 145,-0.1 0.710 125.7 -68.4 63.3 23.9 8.4 -16.8 46.4 70 75 A G T 3 S+ 0 0 69 -3,-0.4 -1,-0.3 1,-0.2 -2,-0.1 0.501 79.8 166.9 75.8 2.8 6.9 -14.4 43.8 71 76 A I < - 0 0 7 -3,-1.9 -1,-0.2 142,-0.1 142,-0.1 -0.289 44.9-111.5 -52.2 122.8 10.0 -12.1 43.9 72 77 A P - 0 0 20 0, 0.0 2,-0.9 0, 0.0 -1,-0.1 -0.378 28.0-128.9 -59.2 141.1 9.6 -9.8 40.9 73 78 A D > - 0 0 21 1,-0.2 4,-2.7 -6,-0.1 5,-0.2 -0.773 24.7-176.8-104.3 91.6 12.3 -10.7 38.4 74 79 A F H > S+ 0 0 2 -2,-0.9 4,-0.7 1,-0.2 -1,-0.2 0.817 84.2 46.2 -48.8 -40.1 14.1 -7.5 37.4 75 80 A F H >4 S+ 0 0 0 2,-0.2 3,-0.7 -13,-0.2 4,-0.2 0.942 113.8 42.7 -79.2 -50.7 16.2 -9.3 34.8 76 81 A K H >4 S+ 0 0 24 1,-0.2 3,-1.2 -14,-0.2 -2,-0.2 0.885 112.8 56.8 -64.1 -35.8 13.6 -11.4 33.1 77 82 A Q H 3< S+ 0 0 54 -4,-2.7 -1,-0.2 1,-0.2 -2,-0.2 0.760 91.5 71.9 -61.9 -28.3 11.3 -8.4 33.0 78 83 A S T - G 0 166A 8 4,-2.9 3,-1.8 -2,-0.6 75,-0.2 -0.636 21.9-125.5 -86.0 144.5 40.4 -8.6 46.7 92 97 A E T 3 S+ 0 0 63 73,-2.6 74,-0.1 -2,-0.3 -1,-0.1 0.580 108.1 56.9 -67.3 -6.3 41.8 -8.3 50.3 93 98 A D T 3 S- 0 0 50 72,-0.2 -1,-0.3 2,-0.1 73,-0.1 0.230 127.7 -85.7-111.2 13.6 45.3 -8.5 48.9 94 99 A G S < S+ 0 0 37 -3,-1.8 -2,-0.1 1,-0.4 25,-0.1 0.061 83.4 129.4 114.7 -24.1 45.2 -5.6 46.5 95 100 A G - 0 0 0 -5,-0.1 -4,-2.9 23,-0.1 2,-0.4 -0.384 33.9-171.6 -62.2 138.3 43.8 -6.9 43.3 96 101 A V E -FH 90 119A 12 23,-2.4 23,-2.5 -6,-0.2 2,-0.5 -0.989 4.0-174.3-133.6 128.8 40.9 -4.9 41.9 97 102 A L E -FH 89 118A 3 -8,-2.7 -8,-2.4 -2,-0.4 2,-0.3 -0.953 2.1-171.4-129.0 115.9 38.8 -6.0 39.0 98 103 A T E -FH 88 117A 0 19,-2.3 19,-2.8 -2,-0.5 2,-0.3 -0.787 7.6-177.6-100.8 147.8 36.1 -4.0 37.3 99 104 A A E -FH 87 116A 2 -12,-1.8 -12,-2.7 -2,-0.3 2,-0.4 -0.997 12.1-165.4-144.3 140.7 33.7 -5.4 34.7 100 105 A T E -FH 86 115A 12 15,-2.1 15,-2.4 -2,-0.3 2,-0.4 -0.993 15.3-171.8-127.1 138.1 30.9 -3.9 32.6 101 106 A Q E -FH 85 114A 8 -16,-3.3 -16,-2.1 -2,-0.4 2,-0.4 -0.980 17.5-156.8-135.8 141.2 28.4 -6.0 30.8 102 107 A D E -FH 84 113A 57 11,-2.2 11,-2.1 -2,-0.4 2,-0.5 -0.972 11.8-165.5-111.3 131.8 25.6 -5.7 28.2 103 108 A T E +FH 83 112A 0 -20,-3.1 -20,-1.8 -2,-0.4 2,-0.3 -0.970 17.6 162.8-118.0 120.4 22.9 -8.3 28.0 104 109 A S E - 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