==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=11-SEP-2011 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 12-JUL-11 3SVS . COMPND 2 MOLECULE: MKATE S158A/S143C; . SOURCE 2 ORGANISM_SCIENTIFIC: ARTIFICIAL GENE; . AUTHOR Q.WANG,L.BYNRES,H.SONDERMANN . 1780 16 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 70613.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 1300 73.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 106 6.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 816 45.8 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 16 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 16 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 24 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 157 8.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 164 9.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 45 2.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 24 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 8 0 0 0 0 0 0 6 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 8 0 0 16 0 8 8 16 8 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 4 A L 0 0 134 0, 0.0 2,-0.9 0, 0.0 75,-0.0 0.000 360.0 360.0 360.0 -34.1 -23.6 16.5 -48.9 2 5 A I - 0 0 12 74,-0.1 4,-0.1 77,-0.1 2,-0.0 -0.846 360.0-161.4 -95.3 104.8 -20.2 15.1 -48.0 3 6 A T - 0 0 73 -2,-0.9 29,-0.2 2,-0.2 3,-0.0 -0.273 34.3-102.3 -83.3 171.2 -20.8 12.9 -45.0 4 7 A E S S+ 0 0 121 1,-0.2 2,-0.4 26,-0.1 28,-0.2 0.743 116.1 38.1 -66.6 -24.1 -18.2 11.7 -42.4 5 8 A N S S+ 0 0 77 25,-0.2 2,-0.3 26,-0.1 -2,-0.2 -0.999 77.9 175.6-124.0 130.1 -18.3 8.4 -44.3 6 9 A M E -A 29 0A 17 23,-2.1 23,-2.1 -2,-0.4 2,-0.3 -0.983 18.7-145.9-134.4 148.1 -18.6 8.2 -48.0 7 10 A H E -A 28 0A 107 101,-0.5 103,-2.5 -2,-0.3 2,-0.3 -0.735 15.3-160.7-104.0 157.4 -18.6 5.5 -50.7 8 11 A M E -Ab 27 110A 1 19,-2.1 19,-1.8 -2,-0.3 2,-0.4 -0.978 10.1-165.1-137.4 151.4 -17.2 5.8 -54.2 9 12 A K E -Ab 26 111A 93 101,-2.3 103,-3.0 -2,-0.3 2,-0.3 -0.997 15.3-167.8-132.6 141.8 -17.5 4.0 -57.5 10 13 A L E -Ab 25 112A 1 15,-2.5 15,-1.9 -2,-0.4 2,-0.4 -0.947 16.2-166.5-136.0 145.5 -15.2 4.4 -60.5 11 14 A Y E +Ab 24 113A 109 101,-2.2 103,-2.7 -2,-0.3 2,-0.4 -0.999 17.9 179.0-129.1 130.0 -15.0 3.5 -64.2 12 15 A M E +Ab 23 114A 3 11,-2.5 11,-2.7 -2,-0.4 2,-0.3 -0.996 6.0 165.3-136.3 137.5 -11.6 3.8 -66.0 13 16 A E E +Ab 22 115A 59 101,-1.8 103,-2.1 -2,-0.4 2,-0.3 -0.993 19.1 99.3-147.0 153.7 -10.6 3.1 -69.6 14 17 A G E -Ab 21 116A 3 7,-2.1 7,-2.5 -2,-0.3 2,-0.3 -0.997 50.9 -85.0 166.0-165.0 -7.6 3.9 -71.8 15 18 A T E -Ab 20 117A 17 101,-1.9 103,-3.1 -2,-0.3 2,-0.4 -0.989 15.4-166.4-136.6 146.9 -4.4 2.9 -73.4 16 19 A V E > S-Ab 19 118A 0 3,-1.8 3,-1.8 -2,-0.3 103,-0.2 -0.981 85.8 -16.6-129.9 119.7 -0.7 2.8 -72.5 17 20 A N T 3 S- 0 0 20 101,-2.6 102,-0.1 -2,-0.4 3,-0.1 0.895 131.4 -50.4 52.1 43.7 1.8 2.3 -75.3 18 21 A N T 3 S+ 0 0 79 100,-0.4 2,-0.7 1,-0.2 -1,-0.3 0.551 109.4 128.6 72.3 9.5 -1.1 1.0 -77.5 19 22 A H E < -A 16 0A 56 -3,-1.8 -3,-1.8 30,-0.0 -1,-0.2 -0.873 49.3-147.6 -98.3 116.4 -2.3 -1.4 -74.8 20 23 A H E +A 15 0A 76 -2,-0.7 2,-0.3 -5,-0.2 -5,-0.2 -0.483 25.6 160.8 -81.0 144.4 -6.0 -1.1 -74.2 21 24 A F E -A 14 0A 0 -7,-2.5 -7,-2.1 -2,-0.2 2,-0.3 -0.990 23.7-157.0-155.9 164.7 -7.5 -1.7 -70.8 22 25 A K E -AC 13 44A 64 22,-2.3 21,-2.4 -2,-0.3 22,-1.7 -0.993 12.0-174.9-143.9 140.4 -10.5 -1.0 -68.6 23 26 A C E -AC 12 42A 0 -11,-2.7 -11,-2.5 -2,-0.3 2,-0.3 -0.929 7.8-165.6-128.7 158.3 -10.9 -1.0 -64.8 24 27 A T E +AC 11 41A 48 17,-2.0 17,-2.8 -2,-0.3 2,-0.3 -0.965 15.1 178.0-132.0 155.5 -13.7 -0.6 -62.3 25 28 A S E -AC 10 40A 7 -15,-1.9 -15,-2.5 -2,-0.3 2,-0.3 -0.995 16.2-168.9-153.4 161.4 -13.5 0.1 -58.6 26 29 A E E +AC 9 39A 87 13,-1.7 13,-2.7 -2,-0.3 2,-0.3 -0.981 24.0 137.1-145.2 151.3 -15.4 0.7 -55.4 27 30 A G E -AC 8 38A 0 -19,-1.8 -19,-2.1 -2,-0.3 2,-0.3 -0.919 32.0-129.1-168.5-168.7 -14.3 1.9 -52.0 28 31 A E E +AC 7 37A 63 9,-1.4 9,-2.5 -2,-0.3 2,-0.3 -0.971 21.7 167.5-155.6 163.9 -15.1 4.1 -49.0 29 32 A G E -AC 6 36A 8 -23,-2.1 -23,-2.1 -2,-0.3 7,-0.3 -0.949 40.9-100.9-164.4-175.5 -13.3 6.7 -46.9 30 33 A K E >>> - C 0 35A 63 5,-2.4 5,-1.4 -2,-0.3 4,-1.2 -0.913 30.3-164.1-123.5 97.8 -13.6 9.4 -44.3 31 34 A P T 345S+ 0 0 0 0, 0.0 -1,-0.1 0, 0.0 -27,-0.1 0.780 86.9 45.0 -63.5 -31.2 -13.4 12.7 -46.2 32 35 A Y T 345S+ 0 0 125 -28,-0.2 -28,-0.1 1,-0.2 32,-0.1 0.645 114.1 50.0 -86.5 -15.5 -12.7 14.9 -43.2 33 36 A E T <45S- 0 0 103 -3,-1.1 33,-0.2 -29,-0.2 -1,-0.2 0.551 107.9-125.7 -95.0 -11.2 -10.0 12.5 -41.8 34 37 A G T <5 + 0 0 0 -4,-1.2 178,-2.3 1,-0.2 2,-0.3 0.745 65.7 125.2 75.6 25.3 -8.3 12.3 -45.2 35 38 A T E < +CD 30 211A 35 -5,-1.4 -5,-2.4 176,-0.2 2,-0.3 -0.894 27.0 157.6-120.9 150.6 -8.4 8.5 -45.5 36 39 A Q E -CD 29 210A 9 174,-1.8 174,-2.3 -2,-0.3 2,-0.4 -0.989 23.6-151.3-166.1 154.5 -9.7 6.1 -48.1 37 40 A T E -CD 28 209A 25 -9,-2.5 -9,-1.4 -2,-0.3 2,-0.4 -0.990 10.4-168.5-130.6 137.8 -9.6 2.6 -49.6 38 41 A M E -CD 27 208A 5 170,-2.6 170,-2.6 -2,-0.4 2,-0.6 -0.996 10.0-155.6-129.2 133.4 -10.3 1.5 -53.2 39 42 A R E -CD 26 207A 53 -13,-2.7 -13,-1.7 -2,-0.4 2,-0.4 -0.947 23.9-164.3-108.8 118.8 -10.7 -2.0 -54.4 40 43 A I E -CD 25 206A 1 166,-3.6 166,-2.5 -2,-0.6 2,-0.4 -0.894 14.3-165.7-117.8 132.7 -9.8 -2.2 -58.1 41 44 A K E -CD 24 205A 113 -17,-2.8 -17,-2.0 -2,-0.4 2,-0.7 -0.962 16.6-144.1-113.5 128.8 -10.5 -4.7 -60.7 42 45 A V E +C 23 0A 15 162,-2.7 -19,-0.3 -2,-0.4 3,-0.1 -0.833 27.5 172.2 -81.7 119.4 -8.8 -4.8 -64.2 43 46 A V E + 0 0 74 -21,-2.4 2,-0.3 -2,-0.7 -20,-0.2 0.663 59.8 28.5-108.0 -22.4 -11.7 -6.0 -66.3 44 47 A E E S+C 22 0A 85 -22,-1.7 -22,-2.3 1,-0.0 -1,-0.3 -0.994 119.2 19.0-135.7 143.0 -10.4 -5.7 -69.9 45 48 A G S S+ 0 0 30 -2,-0.3 -22,-0.0 1,-0.3 -24,-0.0 0.418 94.8 178.7 86.2 0.4 -6.8 -5.8 -71.1 46 49 A G + 0 0 36 -24,-0.1 -1,-0.3 2,-0.1 2,-0.2 -0.512 52.5 47.6 -96.8 162.9 -5.4 -7.6 -68.0 47 50 A P S S- 0 0 79 0, 0.0 156,-0.1 0, 0.0 157,-0.1 0.608 97.4-128.4 -61.1 145.8 -3.1 -8.8 -66.7 48 51 A L - 0 0 2 155,-0.3 4,-0.1 154,-0.2 -2,-0.1 -0.408 3.9-138.9 -73.5 140.7 -1.6 -5.4 -67.8 49 52 A P S S+ 0 0 53 0, 0.0 2,-0.3 0, 0.0 -1,-0.1 -0.004 86.7 52.4 -84.9 27.1 1.7 -5.5 -69.8 50 53 A F S S- 0 0 0 79,-0.1 2,-0.3 80,-0.1 78,-0.2 -0.964 105.0 -71.8-152.2 166.4 2.9 -2.5 -67.8 51 54 A A > - 0 0 1 76,-2.1 3,-1.8 -2,-0.3 4,-0.2 -0.429 30.2-146.4 -68.1 123.6 3.2 -1.6 -64.1 52 55 A F G > S+ 0 0 0 -2,-0.3 3,-2.6 1,-0.3 4,-0.5 0.789 90.8 81.5 -56.0 -28.0 -0.2 -0.9 -62.4 53 56 A D G > S+ 0 0 6 1,-0.3 3,-1.2 2,-0.2 4,-0.4 0.773 79.0 66.0 -50.5 -31.1 1.6 1.7 -60.3 54 57 A I G < S+ 0 0 4 -3,-1.8 -1,-0.3 1,-0.2 -2,-0.2 0.628 103.0 46.9 -72.1 -9.2 1.4 4.2 -63.2 55 58 A L G X S+ 0 0 0 -3,-2.6 3,-2.0 -4,-0.2 4,-0.4 0.471 84.6 97.7-100.6 -7.2 -2.4 4.3 -62.8 56 59 A A G X S+ 0 0 2 -3,-1.2 3,-1.2 -4,-0.5 -2,-0.1 0.839 80.4 49.7 -56.6 -43.4 -2.4 4.6 -59.0 57 60 A T G 3 S+ 0 0 33 -4,-0.4 -1,-0.3 1,-0.2 -2,-0.1 0.427 100.3 68.2 -77.8 1.5 -2.9 8.4 -58.8 58 61 A S G < 0 0 2 -3,-2.0 -1,-0.2 1,-0.1 -2,-0.2 0.550 360.0 360.0 -96.7 -7.9 -5.8 8.2 -61.2 59 62 A F < 0 0 10 -3,-1.2 -2,-0.2 -4,-0.4 -1,-0.1 0.659 360.0 360.0 -92.4 360.0 -8.1 6.3 -58.7 60 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 61 66 A S > 0 0 13 0, 0.0 3,-1.7 0, 0.0 -2,-0.0 0.000 360.0 360.0 360.0 63.5 -10.1 11.2 -55.2 62 67 A K G > + 0 0 2 1,-0.3 3,-0.6 2,-0.1 14,-0.2 0.561 360.0 75.4 -74.3 -14.5 -9.7 14.7 -54.0 63 68 A T G 3 S+ 0 0 0 1,-0.2 2,-0.6 12,-0.1 -1,-0.3 0.758 87.3 68.0 -65.7 -23.2 -12.8 14.8 -51.8 64 69 A F G < S+ 0 0 0 -3,-1.7 2,-0.5 -30,-0.1 148,-0.3 -0.291 70.9 115.2 -96.4 49.7 -10.9 12.7 -49.3 65 70 A I < - 0 0 0 -2,-0.6 2,-1.0 -3,-0.6 148,-0.2 -0.978 66.3-128.4-116.2 128.5 -8.3 15.2 -48.1 66 71 A N E -e 213 0A 50 146,-2.5 148,-3.0 -2,-0.5 2,-1.2 -0.651 22.4-155.6 -73.1 103.4 -8.3 16.5 -44.5 67 72 A H E -e 214 0A 26 -2,-1.0 3,-0.4 146,-0.2 148,-0.1 -0.722 22.1-179.2 -86.1 98.7 -8.2 20.3 -45.0 68 73 A T > + 0 0 38 146,-1.7 3,-2.0 -2,-1.2 145,-0.0 -0.249 52.1 38.4 -89.6 175.2 -6.7 21.5 -41.8 69 74 A Q T 3 S- 0 0 107 1,-0.3 -1,-0.2 -2,-0.1 146,-0.1 0.689 126.1 -69.7 58.9 22.2 -5.9 25.0 -40.5 70 75 A G T 3 S+ 0 0 72 -3,-0.4 -1,-0.3 1,-0.2 -2,-0.1 0.689 79.0 168.0 70.0 17.6 -9.2 26.4 -42.0 71 76 A I < - 0 0 7 -3,-2.0 -1,-0.2 143,-0.1 143,-0.1 -0.494 44.7-109.6 -60.9 123.2 -8.0 26.0 -45.6 72 77 A P - 0 0 32 0, 0.0 2,-1.0 0, 0.0 3,-0.1 -0.380 29.3-131.9 -56.6 134.2 -11.0 26.5 -47.8 73 78 A D > - 0 0 22 1,-0.2 4,-2.5 -6,-0.1 5,-0.2 -0.780 23.6-176.1-101.7 93.2 -11.9 23.1 -49.4 74 79 A F H > S+ 0 0 0 -2,-1.0 4,-0.6 1,-0.2 -1,-0.2 0.836 83.4 47.8 -55.5 -36.2 -12.3 23.7 -53.1 75 80 A F H >4 S+ 0 0 0 2,-0.2 3,-1.1 -13,-0.2 4,-0.3 0.965 113.4 41.3 -76.8 -53.5 -13.3 20.0 -53.6 76 81 A K H >4 S+ 0 0 25 1,-0.3 3,-1.0 -14,-0.2 -2,-0.2 0.848 112.8 57.8 -65.9 -31.2 -15.9 19.6 -50.8 77 82 A Q H 3< S+ 0 0 51 -4,-2.5 -1,-0.3 1,-0.2 -2,-0.2 0.751 91.8 72.2 -64.5 -24.0 -17.3 23.0 -51.6 78 83 A S T - G 0 167A 9 4,-3.1 3,-2.0 -2,-0.6 76,-0.2 -0.578 21.9-123.6 -82.7 146.5 8.5 10.3 -66.3 92 97 A E T 3 S+ 0 0 44 74,-2.6 75,-0.1 1,-0.3 -1,-0.1 0.648 111.2 54.6 -68.2 -13.3 12.2 11.2 -66.3 93 98 A D T 3 S- 0 0 33 73,-0.2 -1,-0.3 2,-0.1 675,-0.1 0.247 129.5 -89.2-102.7 10.7 12.9 8.3 -68.8 94 99 A G S < S+ 0 0 26 -3,-2.0 -2,-0.1 1,-0.3 27,-0.1 0.149 78.5 135.5 115.2 -19.1 10.3 9.6 -71.2 95 100 A G - 0 0 0 -5,-0.1 -4,-3.1 23,-0.1 2,-0.4 -0.360 31.9-166.5 -63.3 141.2 6.9 8.1 -70.5 96 101 A V E -FH 90 119A 11 23,-2.4 23,-2.9 -6,-0.2 2,-0.5 -0.995 5.4-175.1-132.5 127.6 4.0 10.6 -70.6 97 102 A L E -FH 89 118A 4 -8,-2.5 -8,-2.7 -2,-0.4 2,-0.5 -0.978 5.3-166.0-129.5 118.8 0.6 9.8 -69.1 98 103 A T E -FH 88 117A 3 19,-2.7 19,-2.6 -2,-0.5 2,-0.4 -0.898 7.8-172.6-104.6 132.4 -2.4 12.1 -69.4 99 104 A A E -FH 87 116A 1 -12,-2.4 -12,-2.6 -2,-0.5 2,-0.4 -0.987 6.2-167.1-126.1 135.2 -5.5 11.4 -67.2 100 105 A T E -FH 86 115A 21 15,-2.4 15,-2.4 -2,-0.4 2,-0.4 -0.984 14.7-173.9-125.7 130.4 -8.8 13.2 -67.4 101 106 A Q E -FH 85 114A 9 -16,-3.1 -16,-1.9 -2,-0.4 2,-0.4 -0.946 20.0-160.5-133.8 144.8 -11.4 12.8 -64.7 102 107 A D E -FH 84 113A 60 11,-2.1 11,-1.9 -2,-0.4 2,-0.5 -0.986 10.4-164.2-120.4 129.2 -15.0 13.7 -63.8 103 108 A T E +FH 83 112A 0 -20,-2.8 -20,-1.6 -2,-0.4 2,-0.3 -0.958 19.0 160.6-114.2 120.0 -16.2 13.6 -60.2 104 109 A S E - 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