==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=11-SEP-2011 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 12-JUL-11 3SVU . COMPND 2 MOLECULE: MKATE S143C; . SOURCE 2 ORGANISM_SCIENTIFIC: ARTIFICIAL GENE; . AUTHOR Q.WANG,L.BYRNES,H.SONDERMANN . 902 8 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 33178.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 657 72.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 55 6.1 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 405 44.9 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 12 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 91 10.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 72 8.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 25 2.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 11 1.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 4 0 0 0 0 0 0 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 4 0 3 5 0 4 4 8 4 12 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 2 A S 0 0 172 0, 0.0 2,-0.6 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 -59.0 13.4 -4.7 -17.4 2 3 A A + 0 0 77 1,-0.2 3,-0.1 0, 0.0 0, 0.0 -0.807 360.0 177.5 -86.5 119.5 15.2 -7.0 -19.9 3 4 A L + 0 0 79 -2,-0.6 2,-1.0 1,-0.2 -1,-0.2 0.801 66.2 64.3 -96.6 -33.8 14.5 -10.6 -18.7 4 5 A I S S- 0 0 5 74,-0.1 -1,-0.2 77,-0.1 4,-0.1 -0.792 79.3-172.6 -91.0 96.2 16.2 -12.6 -21.4 5 6 A T - 0 0 79 -2,-1.0 29,-0.2 2,-0.2 3,-0.1 -0.164 40.2 -88.0 -88.6-176.4 19.9 -11.6 -20.8 6 7 A E S S+ 0 0 126 1,-0.2 2,-0.4 26,-0.1 28,-0.2 0.799 117.8 34.9 -65.7 -31.8 23.1 -12.4 -22.7 7 8 A N S S- 0 0 89 25,-0.2 2,-0.4 26,-0.1 25,-0.2 -0.985 74.9-179.2-126.4 122.8 23.6 -15.6 -20.9 8 9 A M E -A 31 0A 5 23,-2.5 23,-2.1 -2,-0.4 2,-0.3 -0.934 14.3-143.5-125.4 145.4 20.7 -17.8 -19.8 9 10 A H E -Ab 30 111A 110 101,-0.6 103,-2.4 -2,-0.4 2,-0.3 -0.738 13.3-150.1-106.2 156.3 20.5 -21.1 -17.9 10 11 A M E -Ab 29 112A 2 19,-2.0 19,-1.7 -2,-0.3 2,-0.3 -0.947 16.1-177.2-129.7 146.5 18.2 -24.0 -18.3 11 12 A K E -Ab 28 113A 106 101,-2.0 103,-2.1 -2,-0.3 2,-0.4 -0.980 19.6-175.1-129.7 140.4 16.6 -26.8 -16.2 12 13 A L E -Ab 27 114A 2 15,-2.5 15,-1.9 -2,-0.3 2,-0.4 -0.999 17.6-168.9-143.8 140.3 14.4 -29.4 -17.9 13 14 A Y E -Ab 26 115A 110 101,-2.0 103,-2.4 -2,-0.4 2,-0.4 -0.999 15.0-178.0-126.3 129.7 12.2 -32.3 -16.9 14 15 A M E +Ab 25 116A 5 11,-2.7 11,-2.6 -2,-0.4 2,-0.3 -0.982 6.3 176.0-132.8 142.2 10.8 -34.7 -19.6 15 16 A E E +Ab 24 117A 98 101,-1.3 103,-1.6 -2,-0.4 2,-0.3 -0.995 21.2 113.1-145.4 134.1 8.5 -37.8 -19.4 16 17 A G E -Ab 23 118A 4 7,-2.1 7,-2.1 -2,-0.3 2,-0.3 -0.947 46.2-102.9-172.0-163.9 7.0 -40.0 -22.0 17 18 A T E -Ab 22 119A 2 101,-1.5 103,-2.5 -2,-0.3 2,-0.4 -0.991 14.7-160.9-141.9 141.1 6.6 -43.3 -23.8 18 19 A V E > S-Ab 21 120A 1 3,-1.8 3,-2.3 -2,-0.3 2,-1.2 -0.993 76.2 -36.5-128.1 129.8 7.9 -44.6 -27.1 19 20 A N T 3 S- 0 0 6 101,-1.6 610,-0.1 -2,-0.4 523,-0.0 -0.474 130.4 -32.4 52.0 -93.6 6.1 -47.5 -28.6 20 21 A N T 3 S+ 0 0 75 -2,-1.2 2,-0.6 537,-0.1 -1,-0.3 -0.006 112.5 110.5-138.5 36.0 5.5 -49.1 -25.3 21 22 A H E < -A 18 0A 55 -3,-2.3 -3,-1.8 2,-0.0 2,-0.2 -0.936 53.0-150.7-117.0 116.7 8.6 -48.1 -23.3 22 23 A H E +A 17 0A 83 -2,-0.6 2,-0.3 -5,-0.2 -5,-0.2 -0.487 26.1 156.0 -82.0 149.1 8.2 -45.6 -20.5 23 24 A F E -A 16 0A 2 -7,-2.1 -7,-2.1 -2,-0.2 2,-0.3 -0.989 27.2-148.7-164.4 166.0 11.0 -43.2 -19.5 24 25 A K E -AC 15 46A 74 22,-2.0 21,-1.5 -2,-0.3 22,-1.5 -0.997 13.5-174.4-144.1 144.4 12.1 -39.9 -17.8 25 26 A C E -AC 14 44A 2 -11,-2.6 -11,-2.7 -2,-0.3 2,-0.3 -0.924 8.0-165.1-133.3 158.5 14.9 -37.4 -18.4 26 27 A T E -AC 13 43A 52 17,-2.1 17,-3.3 -2,-0.3 2,-0.3 -0.963 12.4-177.7-134.7 156.4 16.3 -34.3 -16.9 27 28 A S E -AC 12 42A 7 -15,-1.9 -15,-2.5 -2,-0.3 2,-0.3 -0.971 11.3-167.6-155.3 169.5 18.7 -31.9 -18.4 28 29 A E E +AC 11 41A 82 13,-1.0 13,-3.3 -2,-0.3 2,-0.3 -0.972 25.2 131.2-163.1 134.9 20.5 -28.8 -17.5 29 30 A G E -AC 10 40A 0 -19,-1.7 -19,-2.0 -2,-0.3 2,-0.3 -0.904 34.7-126.0-167.8-163.7 22.4 -26.3 -19.6 30 31 A E E +AC 9 39A 68 9,-1.8 9,-2.9 -2,-0.3 2,-0.3 -0.970 21.9 166.6-162.8 160.1 23.2 -22.8 -20.7 31 32 A G E -AC 8 38A 6 -23,-2.1 -23,-2.5 -2,-0.3 7,-0.3 -0.968 41.2-102.9-164.2 173.3 23.3 -20.7 -23.9 32 33 A K E >>> - C 0 37A 76 5,-1.7 5,-1.5 -2,-0.3 3,-1.0 -0.914 29.6-159.4-111.4 102.9 23.4 -17.2 -25.3 33 34 A P T 345S+ 0 0 0 0, 0.0 -1,-0.1 0, 0.0 -27,-0.1 0.748 87.5 46.0 -51.9 -37.1 19.9 -16.4 -26.5 34 35 A Y T 345S+ 0 0 111 -28,-0.2 -28,-0.1 1,-0.2 -27,-0.1 0.672 113.5 48.5 -87.4 -18.6 20.8 -13.5 -28.9 35 36 A E T <45S- 0 0 99 -3,-1.0 33,-0.3 -29,-0.2 -1,-0.2 0.518 107.6-130.8 -92.2 -9.6 23.6 -15.5 -30.5 36 37 A G T <5 + 0 0 0 -4,-0.9 178,-2.0 1,-0.2 2,-0.3 0.830 62.4 127.8 63.5 34.6 21.2 -18.4 -30.8 37 38 A T E < +CD 32 213A 37 -5,-1.5 -5,-1.7 176,-0.2 2,-0.3 -0.914 27.4 160.7-121.8 151.5 23.6 -21.0 -29.3 38 39 A Q E -CD 31 212A 9 174,-1.4 174,-1.9 -2,-0.3 2,-0.4 -0.970 24.5-149.3-166.8 148.2 23.0 -23.4 -26.5 39 40 A T E -CD 30 211A 22 -9,-2.9 -9,-1.8 -2,-0.3 2,-0.4 -0.977 8.8-167.2-125.8 140.2 24.1 -26.6 -24.7 40 41 A M E -CD 29 210A 8 170,-2.4 170,-2.2 -2,-0.4 2,-0.6 -0.984 9.6-156.2-121.8 132.6 22.1 -29.1 -22.8 41 42 A R E -CD 28 209A 54 -13,-3.3 -13,-1.0 -2,-0.4 2,-0.3 -0.938 26.3-164.3-108.4 109.1 23.6 -31.9 -20.6 42 43 A I E -CD 27 208A 2 166,-3.1 166,-1.8 -2,-0.6 2,-0.4 -0.711 15.4-166.8-105.6 141.1 20.9 -34.6 -20.4 43 44 A K E -CD 26 207A 122 -17,-3.3 -17,-2.1 -2,-0.3 2,-1.0 -0.988 19.6-140.5-122.4 131.1 20.4 -37.6 -18.1 44 45 A V E +C 25 0A 18 162,-2.6 -19,-0.3 -2,-0.4 4,-0.2 -0.798 29.1 174.3 -84.9 104.9 18.0 -40.5 -18.7 45 46 A V E + 0 0 82 -21,-1.5 2,-0.3 -2,-1.0 -20,-0.2 0.482 58.8 29.9 -98.1 -2.6 16.8 -40.9 -15.1 46 47 A E E S+C 24 0A 94 -22,-1.5 -22,-2.0 1,-0.0 -1,-0.2 -0.923 116.4 14.6-158.6 129.6 14.2 -43.6 -15.6 47 48 A G S S- 0 0 34 -2,-0.3 -2,-0.1 1,-0.3 -1,-0.0 0.676 88.1-176.9 82.7 19.1 13.7 -46.5 -18.1 48 49 A G + 0 0 38 -4,-0.2 -1,-0.3 2,-0.1 2,-0.2 -0.753 56.0 54.8-103.9 155.0 17.3 -46.5 -19.5 49 50 A P S S- 0 0 76 0, 0.0 156,-0.1 0, 0.0 155,-0.1 0.447 87.8-141.5 -61.8 142.7 18.9 -47.9 -21.4 50 51 A L - 0 0 2 154,-0.4 -2,-0.1 -2,-0.2 4,-0.1 -0.553 5.5-137.5 -75.3 141.3 16.2 -46.8 -23.9 51 52 A P S S+ 0 0 47 0, 0.0 2,-0.3 0, 0.0 81,-0.2 0.322 82.0 64.8 -84.8 12.2 15.5 -49.4 -26.6 52 53 A F S S- 0 0 0 79,-0.1 78,-0.2 80,-0.1 -2,-0.1 -0.918 97.7 -84.1-131.5 157.5 15.4 -46.7 -29.3 53 54 A A > - 0 0 0 76,-2.6 3,-1.1 -2,-0.3 4,-0.1 -0.354 31.9-138.0 -57.6 132.6 17.8 -44.2 -30.9 54 55 A F G >> S+ 0 0 1 1,-0.2 3,-2.8 2,-0.1 4,-0.9 0.771 91.4 83.6 -63.8 -27.8 18.1 -41.1 -28.8 55 56 A D G 34 S+ 0 0 5 1,-0.3 4,-0.5 2,-0.2 3,-0.3 0.760 79.3 65.9 -46.6 -31.8 18.0 -38.9 -32.0 56 57 A I G <4 S+ 0 0 2 -3,-1.1 -1,-0.3 1,-0.2 -2,-0.1 0.615 103.7 45.1 -74.7 -11.5 14.2 -39.1 -32.0 57 58 A L T X4 S+ 0 0 0 -3,-2.8 3,-2.1 -4,-0.1 4,-0.2 0.658 87.7 94.3 -97.3 -21.4 14.0 -37.1 -28.8 58 59 A A G >< S+ 0 0 4 -4,-0.9 3,-1.7 -3,-0.3 -2,-0.1 0.811 79.5 53.4 -40.2 -56.5 16.6 -34.5 -29.9 59 60 A T G 3 S+ 0 0 31 -4,-0.5 -1,-0.3 1,-0.3 -3,-0.1 0.226 101.6 66.6 -69.2 18.0 14.2 -31.9 -31.2 60 61 A S G < 0 0 3 -3,-2.1 -1,-0.3 1,-0.1 -2,-0.2 0.418 360.0 360.0-120.3 -3.1 12.4 -32.0 -27.9 61 62 A F < 0 0 9 -3,-1.7 -2,-0.1 -4,-0.2 -1,-0.1 0.264 360.0 360.0 -96.7 360.0 15.0 -30.5 -25.5 62 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 63 66 A S > 0 0 13 0, 0.0 3,-1.1 0, 0.0 -2,-0.0 0.000 360.0 360.0 360.0 71.2 14.5 -24.5 -26.8 64 67 A K T 3 + 0 0 3 1,-0.3 3,-0.4 2,-0.1 13,-0.2 0.616 360.0 72.8 -75.5 -16.8 13.0 -21.7 -28.9 65 68 A T T 3 S+ 0 0 0 1,-0.2 2,-1.1 12,-0.1 -1,-0.3 0.797 86.2 68.1 -63.9 -30.6 14.2 -18.8 -26.8 66 69 A F S < S+ 0 0 0 -3,-1.1 2,-0.5 -30,-0.1 148,-0.3 -0.351 70.4 120.8 -92.8 54.3 17.8 -19.3 -28.1 67 70 A I - 0 0 0 -2,-1.1 2,-1.0 -3,-0.4 148,-0.2 -0.981 65.1-125.9-117.4 130.0 17.2 -18.2 -31.7 68 71 A N E -e 215 0A 51 146,-2.5 148,-1.9 -2,-0.5 2,-1.5 -0.587 22.8-159.1 -71.3 99.5 19.2 -15.3 -33.2 69 72 A H E -e 216 0A 29 -2,-1.0 3,-0.5 146,-0.2 148,-0.1 -0.661 20.5-176.8 -87.7 89.1 16.3 -13.1 -34.6 70 73 A T > + 0 0 39 -2,-1.5 3,-1.4 146,-1.0 148,-0.0 -0.264 55.1 35.7 -77.6 165.4 18.3 -11.1 -37.1 71 74 A Q T 3 S- 0 0 104 1,-0.3 -1,-0.2 146,-0.1 146,-0.1 0.658 126.3 -73.1 68.8 17.3 17.0 -8.3 -39.3 72 75 A G T 3 S+ 0 0 67 -3,-0.5 -1,-0.3 1,-0.2 -2,-0.2 0.855 76.5 172.3 61.8 33.9 14.7 -7.0 -36.5 73 76 A I < - 0 0 5 -3,-1.4 -1,-0.2 143,-0.1 2,-0.1 -0.653 45.2-104.3 -70.6 124.4 12.4 -9.9 -37.0 74 77 A P - 0 0 28 0, 0.0 2,-1.0 0, 0.0 -1,-0.1 -0.325 31.8-132.9 -58.3 126.6 9.9 -9.7 -34.2 75 78 A D > - 0 0 21 1,-0.2 4,-1.9 -6,-0.1 5,-0.1 -0.716 23.4-176.4 -91.8 101.0 10.8 -12.3 -31.6 76 79 A F H > S+ 0 0 1 -2,-1.0 4,-0.7 1,-0.2 -1,-0.2 0.838 82.8 45.8 -64.6 -35.2 7.6 -14.1 -30.6 77 80 A F H 4 S+ 0 0 0 2,-0.2 3,-0.3 -13,-0.2 -1,-0.2 0.893 113.3 44.8 -81.6 -43.3 9.4 -16.3 -28.0 78 81 A K H >4 S+ 0 0 28 1,-0.2 3,-1.1 -14,-0.2 -2,-0.2 0.858 111.8 55.7 -68.1 -32.0 11.5 -13.7 -26.3 79 82 A Q H 3< S+ 0 0 52 -4,-1.9 -1,-0.2 1,-0.2 -2,-0.2 0.769 90.5 74.7 -67.6 -27.4 8.5 -11.3 -26.2 80 83 A S T 3X S+ 0 0 0 -4,-0.7 4,-2.6 -3,-0.3 3,-0.5 0.508 82.9 143.7 -72.6 -3.7 6.4 -13.9 -24.3 81 84 A F T <4 + 0 0 9 -3,-1.1 -78,-0.1 1,-0.3 -77,-0.1 -0.533 49.7 22.0 -86.8 156.5 8.2 -13.3 -21.1 82 85 A P T 4 S+ 0 0 78 0, 0.0 25,-0.6 0, 0.0 -1,-0.3 -0.997 132.0 46.0 -80.9 1.1 7.8 -13.2 -18.2 83 86 A E T 4 S- 0 0 74 -3,-0.5 -2,-0.3 1,-0.2 2,-0.1 0.849 95.2-159.2 -72.8 -33.6 4.7 -15.3 -18.9 84 87 A G < - 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