==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=12-DEC-2009 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER BIOTIN-BINDING PROTEIN 04-MAR-97 1SWC . COMPND 2 MOLECULE: STREPTAVIDIN; . SOURCE 2 ORGANISM_SCIENTIFIC: STREPTOMYCES AVIDINII; . AUTHOR S.FREITAG,I.LE TRONG,L.KLUMB,P.S.STAYTON,R.E.STENKAMP . 461 6 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 19047.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 325 70.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 242 52.5 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 9 2.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 45 9.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 33 7.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 4 2 0 6 3 4 3 0 8 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 16 A G 0 0 69 0, 0.0 84,-0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0-124.5 -13.6 17.3 -5.8 2 17 A I + 0 0 15 77,-0.1 14,-0.2 2,-0.0 85,-0.0 0.871 360.0 98.3 -76.0 -38.8 -13.8 19.2 -2.5 3 18 A T + 0 0 68 15,-0.1 2,-0.3 12,-0.1 14,-0.2 -0.237 66.5 60.1 -51.1 131.8 -16.2 22.0 -3.6 4 19 A G E S-A 16 0A 23 12,-2.4 12,-2.7 111,-0.1 2,-0.5 -0.974 89.7 -45.5 144.6-162.7 -19.8 21.2 -2.5 5 20 A T E -A 15 0A 54 -2,-0.3 2,-0.3 10,-0.2 10,-0.2 -0.937 49.5-169.1-115.4 128.2 -22.0 20.5 0.5 6 21 A W E -A 14 0A 0 8,-3.0 8,-3.1 -2,-0.5 2,-0.3 -0.938 6.1-163.0-124.4 148.4 -20.8 18.1 3.2 7 22 A Y E -AB 13 114A 111 107,-2.4 107,-2.5 -2,-0.3 2,-0.3 -0.943 9.5-146.1-127.2 140.5 -22.5 16.5 6.2 8 23 A N E > - B 0 113A 16 4,-1.8 3,-2.2 -2,-0.3 105,-0.2 -0.742 37.6 -90.6-104.7 166.2 -21.1 14.9 9.3 9 24 A Q T 3 S+ 0 0 91 103,-1.0 104,-0.1 -2,-0.3 -1,-0.1 0.658 122.9 44.4 -24.4 -40.2 -22.1 12.0 11.5 10 25 A L T 3 S- 0 0 125 2,-0.0 -1,-0.3 0, 0.0 101,-0.0 0.016 120.3 -90.0-119.7 34.7 -24.1 14.5 13.7 11 26 A G S < S+ 0 0 64 -3,-2.2 -2,-0.1 1,-0.3 2,-0.1 0.325 75.8 136.5 88.8 -11.5 -26.1 16.8 11.5 12 27 A S - 0 0 38 -4,-0.1 -4,-1.8 16,-0.0 2,-0.4 -0.392 38.5-152.0 -72.4 154.5 -23.6 19.7 10.9 13 28 A T E -A 7 0A 31 -6,-0.2 16,-3.7 -2,-0.1 2,-0.4 -0.979 11.3-170.4-130.0 134.4 -23.4 21.0 7.4 14 29 A F E -AC 6 28A 2 -8,-3.1 -8,-3.0 -2,-0.4 2,-0.5 -0.993 5.5-163.2-131.3 126.4 -20.4 22.7 5.7 15 30 A I E +AC 5 27A 60 12,-2.0 12,-1.6 -2,-0.4 2,-0.4 -0.936 23.8 177.6-107.8 116.5 -20.6 24.4 2.3 16 31 A V E -AC 4 26A 1 -12,-2.7 -12,-2.4 -2,-0.5 2,-0.4 -0.925 25.9-159.6-131.1 152.6 -17.2 24.9 0.8 17 32 A T E - C 0 25A 73 8,-3.3 8,-1.7 -2,-0.4 2,-0.6 -0.987 13.4-149.7-131.9 123.3 -15.5 26.2 -2.2 18 33 A A E - C 0 24A 21 -2,-0.4 6,-0.2 6,-0.2 -15,-0.1 -0.798 14.9-150.5 -92.1 113.2 -12.0 25.3 -3.2 19 34 A G > - 0 0 22 4,-3.2 3,-1.2 -2,-0.6 -16,-0.0 -0.385 25.1-118.8 -75.4 167.4 -10.1 28.0 -5.1 20 35 A A T 3 S+ 0 0 100 1,-0.3 -1,-0.1 2,-0.1 25,-0.1 0.659 113.9 68.9 -81.8 -11.0 -7.4 27.1 -7.6 21 36 A D T 3 S- 0 0 101 2,-0.1 -1,-0.3 21,-0.0 3,-0.1 0.290 124.8-100.7 -77.8 -8.3 -5.2 29.1 -5.2 22 37 A G S < S+ 0 0 1 -3,-1.2 21,-2.2 1,-0.3 2,-0.2 0.496 79.0 143.2 91.0 5.1 -5.4 26.5 -2.5 23 38 A A E - D 0 42A 21 19,-0.2 -4,-3.2 22,-0.0 2,-0.4 -0.518 36.4-160.8 -81.1 138.3 -8.0 28.6 -0.7 24 39 A L E +CD 18 41A 2 17,-2.2 17,-2.1 -6,-0.2 2,-0.3 -0.993 18.2 165.7-116.6 131.5 -11.0 27.1 1.1 25 40 A T E +CD 17 40A 75 -8,-1.7 -8,-3.3 -2,-0.4 2,-0.3 -0.945 17.7 100.5-139.6 158.5 -14.0 29.3 1.9 26 41 A G E -CD 16 39A 21 13,-1.1 13,-2.3 -2,-0.3 2,-0.3 -0.981 57.8 -81.1 162.1-157.3 -17.6 28.5 3.0 27 42 A T E -CD 15 38A 39 -12,-1.6 -12,-2.0 -2,-0.3 2,-0.5 -0.960 20.3-137.6-139.4 148.9 -19.9 28.4 6.0 28 43 A Y E CD 14 37A 13 9,-3.1 9,-2.6 -2,-0.3 -14,-0.2 -0.981 360.0 360.0-110.8 124.9 -20.5 25.9 8.7 29 44 A E 0 0 48 -16,-3.7 3,-0.2 -2,-0.5 -15,-0.2 0.363 360.0 360.0-135.3 360.0 -24.2 25.6 9.3 30 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 31 48 A G 0 0 69 0, 0.0 2,-0.2 0, 0.0 5,-0.1 0.000 360.0 360.0 360.0-109.0 -29.9 26.6 7.2 32 49 A N > - 0 0 84 -3,-0.2 3,-2.2 1,-0.1 4,-0.3 -0.550 360.0-122.4 -84.1 139.7 -30.9 29.2 9.7 33 50 A A G > S+ 0 0 37 1,-0.3 3,-1.6 -2,-0.2 33,-0.2 0.817 106.1 58.0 -47.6 -39.7 -28.9 32.4 10.0 34 51 A E G 3 S+ 0 0 84 1,-0.3 -1,-0.3 32,-0.1 30,-0.1 0.614 105.7 50.9 -80.7 -0.3 -28.1 32.1 13.7 35 52 A S G < S+ 0 0 44 -3,-2.2 2,-0.5 -6,-0.1 -1,-0.3 0.295 90.1 99.6-107.1 4.4 -26.4 28.7 13.2 36 53 A R < + 0 0 102 -3,-1.6 2,-0.3 -4,-0.3 -7,-0.2 -0.829 45.5 177.3-102.4 133.0 -24.1 29.8 10.4 37 54 A Y E -D 28 0A 27 -9,-2.6 -9,-3.1 -2,-0.5 2,-0.2 -0.960 31.3-108.8-132.5 149.9 -20.5 30.7 10.8 38 55 A V E -D 27 0A 72 -2,-0.3 23,-0.6 25,-0.3 2,-0.4 -0.530 33.7-163.3 -76.0 137.8 -17.8 31.7 8.3 39 56 A L E +DE 26 60A 2 -13,-2.3 -13,-1.1 -2,-0.2 2,-0.3 -0.923 10.5 179.4-118.3 152.7 -15.0 29.2 7.6 40 57 A T E +DE 25 59A 18 19,-1.9 19,-2.6 -2,-0.4 2,-0.3 -0.993 16.2 132.9-145.4 150.4 -11.7 29.6 6.0 41 58 A G E -DE 24 58A 0 -17,-2.1 -17,-2.2 -2,-0.3 2,-0.3 -0.895 37.7-108.0-165.9-166.3 -8.9 27.2 5.3 42 59 A R E -DE 23 57A 30 15,-1.9 15,-2.3 -2,-0.3 2,-0.3 -0.963 20.9-171.7-139.7 152.4 -6.3 25.8 2.9 43 60 A Y E - E 0 56A 29 -21,-2.2 2,-0.8 -2,-0.3 13,-0.2 -0.962 38.6 -94.1-141.6 165.3 -5.8 22.6 0.9 44 61 A D - 0 0 2 11,-2.5 -22,-0.1 -2,-0.3 146,-0.0 -0.764 34.1-168.9 -76.1 107.5 -3.1 20.9 -1.2 45 62 A S S S+ 0 0 42 -2,-0.8 -1,-0.1 1,-0.2 -23,-0.1 0.285 81.5 40.1 -84.2 4.9 -4.1 22.0 -4.8 46 63 A A S S- 0 0 52 -24,-0.0 -1,-0.2 0, 0.0 3,-0.1 -0.472 83.4-176.1-149.6 67.6 -1.6 19.5 -6.4 47 64 A P - 0 0 47 0, 0.0 7,-0.1 0, 0.0 6,-0.0 -0.100 36.0 -81.4 -63.0 163.5 -1.8 16.3 -4.4 48 65 A A - 0 0 29 5,-0.3 2,-0.5 1,-0.1 3,-0.4 -0.213 32.7-135.8 -59.9 149.0 0.3 13.2 -5.0 49 66 A T S S+ 0 0 144 1,-0.2 -1,-0.1 -3,-0.1 0, 0.0 -0.416 79.6 89.2-108.8 57.9 -0.8 10.9 -7.7 50 67 A D S S- 0 0 104 -2,-0.5 -1,-0.2 2,-0.1 3,-0.1 0.172 113.0 -78.9-137.6 13.1 -0.4 7.5 -5.9 51 68 A G S S+ 0 0 55 -3,-0.4 166,-0.3 1,-0.3 164,-0.1 0.366 88.4 136.4 97.9 -1.9 -3.7 6.9 -4.2 52 69 A S - 0 0 27 164,-0.1 -1,-0.3 162,-0.1 164,-0.3 -0.395 53.9-114.1 -74.3 154.6 -2.9 9.3 -1.3 53 70 A G - 0 0 8 162,-2.2 2,-0.6 160,-0.1 -5,-0.3 -0.191 30.4 -97.0 -80.3-179.1 -5.6 11.6 -0.1 54 71 A T E - F 0 79A 17 25,-2.4 25,-2.7 -7,-0.1 2,-0.1 -0.910 34.5-133.3-105.0 113.6 -5.6 15.4 -0.3 55 72 A A E + F 0 78A 1 -2,-0.6 -11,-2.5 23,-0.2 2,-0.3 -0.342 37.6 152.8 -67.3 142.7 -4.5 17.3 2.8 56 73 A L E -EF 43 77A 1 21,-2.2 21,-2.3 -13,-0.2 2,-0.3 -0.984 20.8-169.9-162.1 164.9 -6.5 20.2 4.0 57 74 A G E -EF 42 76A 0 -15,-2.3 -15,-1.9 -2,-0.3 2,-0.3 -0.967 5.3-176.8-159.2 152.9 -7.5 22.2 7.1 58 75 A W E -EF 41 75A 1 17,-1.6 17,-2.8 -2,-0.3 2,-0.4 -0.964 12.6-142.0-149.9 166.3 -10.0 24.9 8.0 59 76 A T E -EF 40 74A 0 -19,-2.6 -19,-1.9 -2,-0.3 2,-0.4 -0.980 6.8-169.5-132.2 143.1 -11.0 27.1 11.0 60 77 A V E -EF 39 73A 0 13,-2.3 13,-3.0 -2,-0.4 2,-0.5 -0.998 9.6-157.0-131.6 120.7 -14.3 28.3 12.3 61 78 A A E - F 0 72A 3 -23,-0.6 2,-1.8 -2,-0.4 11,-0.2 -0.914 24.5-130.3 -93.5 127.7 -14.5 31.0 15.0 62 79 A W E S+ 0 0 48 9,-2.5 7,-2.7 -2,-0.5 2,-0.4 -0.417 71.8 103.5 -85.7 62.5 -17.8 30.6 16.8 63 80 A K E + F 0 68A 66 -2,-1.8 -25,-0.3 5,-0.2 5,-0.3 -0.983 36.8 153.6-138.0 124.5 -19.0 34.2 16.6 64 81 A N - 0 0 21 3,-3.0 -27,-0.1 -2,-0.4 -30,-0.1 -0.713 64.5 -76.3-131.2-167.8 -21.6 35.4 14.2 65 82 A N S S+ 0 0 137 -2,-0.2 -31,-0.1 1,-0.2 3,-0.1 0.752 130.7 42.0 -58.1 -25.2 -24.1 38.3 14.2 66 83 A Y S S+ 0 0 140 1,-0.2 2,-0.3 -33,-0.2 -1,-0.2 0.838 119.6 5.1 -99.2 -37.1 -26.2 36.2 16.6 67 84 A R - 0 0 120 -33,-0.1 -3,-3.0 2,-0.0 2,-0.3 -0.923 45.4-154.1-152.2 166.0 -23.9 34.6 19.1 68 85 A N E +F 63 0A 47 -2,-0.3 -5,-0.2 -5,-0.3 -6,-0.0 -0.903 9.9 177.2-147.7 111.2 -20.4 34.3 20.5 69 86 A A E - 0 0 60 -7,-2.7 27,-0.2 -2,-0.3 -6,-0.1 0.344 45.7-125.8 -96.1 8.4 -19.0 31.2 22.3 70 87 A H E S+ 0 0 60 -8,-0.3 26,-2.7 1,-0.2 2,-0.3 0.930 75.4 100.0 45.1 53.5 -15.5 32.8 22.6 71 88 A S E - G 0 95A 10 24,-0.2 -9,-2.5 102,-0.1 2,-0.3 -0.985 54.7-152.7-158.3 163.9 -14.0 29.7 21.0 72 89 A A E -FG 61 94A 0 22,-2.2 22,-2.4 -2,-0.3 2,-0.4 -1.000 6.6-149.5-144.6 144.6 -12.7 28.4 17.7 73 90 A T E -FG 60 93A 9 -13,-3.0 -13,-2.3 -2,-0.3 2,-0.4 -0.941 6.5-163.9-116.3 138.9 -12.4 25.0 16.1 74 91 A T E -FG 59 92A 0 18,-2.6 18,-2.2 -2,-0.4 2,-0.5 -0.991 5.6-158.7-117.2 136.8 -9.7 23.9 13.7 75 92 A W E -FG 58 91A 6 -17,-2.8 -17,-1.6 -2,-0.4 2,-0.4 -0.930 7.5-174.4-114.3 131.4 -10.1 20.8 11.6 76 93 A S E +FG 57 90A 0 14,-3.2 14,-2.4 -2,-0.5 2,-0.3 -0.987 33.0 96.7-124.3 128.5 -7.1 19.0 10.1 77 94 A G E -FG 56 89A 2 -21,-2.3 -21,-2.2 -2,-0.4 2,-0.3 -0.862 56.1 -83.4-175.4-154.5 -7.6 16.0 7.7 78 95 A Q E -FG 55 88A 9 10,-2.2 10,-2.2 -2,-0.3 2,-0.4 -0.997 21.0-125.8-146.3 154.3 -7.8 14.8 4.2 79 96 A Y E -FG 54 87A 37 -25,-2.7 -25,-2.4 -2,-0.3 2,-0.4 -0.802 20.3-168.8 -97.2 136.5 -10.1 14.4 1.3 80 97 A V E - 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