==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=10-JUN-2012 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER BIOTIN-BINDING PROTEIN 23-JUL-11 3T2X . COMPND 2 MOLECULE: AVIDIN/STREPTAVIDIN; . SOURCE 2 ORGANISM_SCIENTIFIC: SHEWANELLA DENITRIFICANS; . AUTHOR O.LIVNAH,A.MEIR . 474 4 4 4 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 19145.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 343 72.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 8 1.7 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 252 53.2 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 1.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 12 2.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 46 9.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 35 7.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 2 2 4 1 8 3 8 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 3 A M 0 0 200 0, 0.0 3,-0.1 0, 0.0 87,-0.0 0.000 360.0 360.0 360.0 128.5 -14.4 14.1 -27.6 2 4 A A - 0 0 13 1,-0.1 2,-0.2 86,-0.1 86,-0.2 -0.145 360.0-107.6 -42.2 131.2 -16.8 13.0 -24.8 3 5 A Q - 0 0 35 84,-0.2 2,-0.2 86,-0.1 114,-0.1 -0.495 37.3-156.9 -66.2 138.5 -14.9 12.1 -21.7 4 6 A E - 0 0 107 -2,-0.2 2,-1.5 85,-0.1 85,-0.1 -0.550 37.8 -78.2-106.6 175.3 -14.8 8.3 -20.9 5 7 A L S S+ 0 0 24 -2,-0.2 2,-0.3 51,-0.1 51,-0.1 -0.628 81.7 136.9 -77.1 90.0 -14.3 6.5 -17.6 6 8 A T > - 0 0 58 -2,-1.5 3,-0.6 1,-0.1 15,-0.1 -0.859 63.2 -80.0-128.7 165.8 -10.5 7.0 -17.4 7 9 A A T 3 S+ 0 0 46 -2,-0.3 16,-0.2 1,-0.2 15,-0.2 -0.340 116.6 26.6 -53.5 148.0 -7.8 7.8 -14.9 8 10 A M T 3 S+ 0 0 147 14,-1.2 2,-0.4 13,-0.7 -1,-0.2 0.903 89.5 137.1 57.7 44.8 -7.7 11.6 -14.4 9 11 A S E < -A 21 0A 3 -3,-0.6 12,-2.7 12,-0.6 2,-0.3 -0.966 37.5-155.6-119.0 141.3 -11.4 12.2 -15.3 10 12 A A E -A 20 0A 19 108,-0.5 108,-1.8 -2,-0.4 2,-0.4 -0.919 7.9-171.8-121.6 148.4 -13.7 14.5 -13.3 11 13 A W E -AB 19 117A 0 8,-2.2 8,-2.8 -2,-0.3 2,-0.4 -1.000 7.0-156.9-145.9 130.3 -17.4 14.5 -13.0 12 14 A V E -AB 18 116A 33 104,-2.8 104,-2.7 -2,-0.4 6,-0.2 -0.917 12.6-147.5-117.6 133.2 -19.8 17.0 -11.3 13 15 A N > - 0 0 11 4,-2.8 3,-1.8 -2,-0.4 102,-0.2 -0.202 37.1 -86.3 -94.5-174.9 -23.3 16.2 -10.0 14 16 A Q T 3 S+ 0 0 40 239,-0.4 240,-0.1 100,-0.3 101,-0.1 0.667 127.1 52.2 -67.7 -17.0 -26.5 18.2 -9.8 15 17 A D T 3 S- 0 0 64 2,-0.1 -1,-0.3 238,-0.1 24,-0.2 0.443 120.9-104.9 -94.2 -4.4 -25.5 19.6 -6.4 16 18 A G S < S+ 0 0 26 -3,-1.8 2,-0.2 1,-0.3 -2,-0.1 0.514 73.8 142.2 88.7 8.9 -22.1 20.9 -7.7 17 19 A S - 0 0 0 19,-0.1 -4,-2.8 20,-0.0 2,-0.5 -0.560 39.0-142.4 -84.5 146.4 -20.1 18.1 -6.0 18 20 A T E -AC 12 35A 8 17,-2.3 17,-2.5 -6,-0.2 2,-0.6 -0.947 13.8-155.4-121.8 132.2 -17.1 16.6 -7.9 19 21 A L E -AC 11 34A 0 -8,-2.8 -8,-2.2 -2,-0.5 2,-0.7 -0.892 14.6-163.2 -92.9 120.0 -15.7 13.1 -8.2 20 22 A Y E -AC 10 33A 86 13,-3.2 13,-2.2 -2,-0.6 2,-0.6 -0.912 6.7-155.3-106.0 101.8 -12.0 13.4 -9.2 21 23 A I E +AC 9 32A 4 -12,-2.7 -13,-0.7 -2,-0.7 -12,-0.6 -0.706 17.4 174.6 -79.1 124.0 -10.9 10.0 -10.5 22 24 A N E + 0 0 87 9,-2.5 -14,-1.2 -2,-0.6 2,-0.3 0.902 58.8 6.8 -90.4 -71.3 -7.1 9.7 -10.0 23 25 A S E - C 0 31A 43 8,-0.7 8,-2.2 -16,-0.2 2,-0.4 -0.835 44.2-174.3-118.9 149.5 -6.2 6.2 -11.1 24 26 A I E - C 0 30A 38 -2,-0.3 -17,-0.2 6,-0.2 6,-0.2 -0.939 28.5-152.8-134.5 109.1 -7.9 3.3 -12.7 25 27 A N > - 0 0 69 4,-2.4 3,-2.1 -2,-0.4 -18,-0.0 -0.055 35.3 -84.4 -80.9-177.6 -5.5 0.3 -12.7 26 28 A A T 3 S+ 0 0 97 1,-0.3 -1,-0.1 2,-0.1 0, 0.0 0.754 131.0 49.3 -62.1 -23.4 -5.3 -2.6 -15.1 27 29 A Q T 3 S- 0 0 125 2,-0.1 -1,-0.3 28,-0.0 3,-0.1 0.452 121.4-106.6 -91.2 -1.0 -8.0 -4.5 -13.1 28 30 A G < + 0 0 7 -3,-2.1 26,-2.2 1,-0.3 2,-0.3 0.560 65.9 154.0 83.1 13.1 -10.3 -1.5 -13.1 29 31 A E E - D 0 53A 63 24,-0.2 -4,-2.4 1,-0.0 2,-0.3 -0.593 31.4-147.6 -77.8 133.1 -9.7 -0.6 -9.4 30 32 A L E -CD 24 52A 0 22,-2.3 22,-1.1 -2,-0.3 2,-0.3 -0.793 21.5-179.1-109.3 142.8 -10.3 3.1 -8.9 31 33 A T E +CD 23 51A 52 -8,-2.2 -9,-2.5 -2,-0.3 -8,-0.7 -0.956 26.0 120.5-127.8 153.4 -8.8 5.7 -6.6 32 34 A G E -CD 21 50A 10 18,-1.7 18,-2.4 -2,-0.3 2,-0.3 -0.973 47.4 -95.5-177.2-164.1 -9.8 9.4 -6.3 33 35 A S E -CD 20 49A 50 -13,-2.2 -13,-3.2 -2,-0.3 2,-0.4 -0.925 21.8-151.0-135.0 159.1 -11.1 12.2 -4.3 34 36 A Y E -CD 19 48A 7 14,-2.1 14,-2.3 -2,-0.3 2,-0.5 -0.998 2.9-164.1-133.8 136.3 -14.5 13.8 -3.7 35 37 A I E -CD 18 47A 32 -17,-2.5 -17,-2.3 -2,-0.4 2,-0.4 -0.981 14.6-148.7-121.4 118.1 -15.5 17.3 -2.9 36 38 A N B +i 44 0B 17 7,-0.5 9,-2.4 10,-0.5 10,-0.2 -0.695 26.3 163.6 -83.8 131.7 -19.0 17.8 -1.6 37 39 A R + 0 0 82 -2,-0.4 2,-0.2 7,-0.2 -1,-0.1 0.121 21.9 145.1-131.4 20.7 -20.7 21.2 -2.4 38 40 A A > - 0 0 15 4,-0.1 3,-2.1 1,-0.1 -22,-0.1 -0.437 59.1-105.0 -67.1 128.6 -24.4 20.3 -1.7 39 41 A A T 3 S+ 0 0 101 1,-0.3 -1,-0.1 -2,-0.2 3,-0.1 -0.236 101.5 8.5 -55.5 130.4 -26.2 23.3 -0.3 40 42 A G T 3 S+ 0 0 87 1,-0.3 -1,-0.3 -3,-0.1 2,-0.1 0.383 100.3 126.6 80.2 -3.0 -26.8 22.9 3.5 41 43 A A < - 0 0 39 -3,-2.1 3,-0.3 1,-0.1 -1,-0.3 -0.464 56.3-126.4 -84.0 157.7 -24.7 19.8 3.8 42 44 A A S S+ 0 0 58 1,-0.2 -4,-0.1 -2,-0.1 30,-0.1 -0.370 88.1 45.8 -84.8 176.4 -21.8 19.2 6.3 43 45 A a S S+ 0 0 1 1,-0.2 -7,-0.5 28,-0.2 3,-0.3 0.894 81.8 158.7 54.2 46.9 -18.4 18.0 5.0 44 46 A Q B +i 36 0B 55 -3,-0.3 -7,-0.2 1,-0.2 -1,-0.2 -0.664 47.6 13.1 -95.0 155.6 -18.3 20.6 2.2 45 47 A N S S+ 0 0 117 -9,-2.4 -1,-0.2 -2,-0.3 -8,-0.1 0.652 99.1 95.3 65.2 20.5 -15.3 22.0 0.3 46 48 A S S S- 0 0 10 -3,-0.3 -10,-0.5 -10,-0.2 2,-0.3 -0.972 78.1 -99.6-139.1 153.9 -12.6 19.6 1.4 47 49 A P E -D 35 0A 78 0, 0.0 -12,-0.2 0, 0.0 -3,-0.0 -0.595 32.8-169.2 -83.3 134.0 -11.3 16.4 -0.3 48 50 A Y E -D 34 0A 2 -14,-2.3 -14,-2.1 -2,-0.3 19,-0.1 -0.939 29.1-102.7-115.4 152.0 -12.5 13.0 0.8 49 51 A P E -D 33 0A 45 0, 0.0 15,-0.9 0, 0.0 2,-0.4 -0.305 35.3-165.6 -80.4 143.7 -11.0 9.7 -0.2 50 52 A V E +DE 32 63A 5 -18,-2.4 -18,-1.7 13,-0.2 2,-0.3 -0.971 13.3 168.4-129.0 141.0 -12.6 7.5 -2.8 51 53 A N E +DE 31 62A 2 11,-1.9 11,-2.5 -2,-0.4 2,-0.3 -0.858 21.2 114.2-133.6 174.6 -12.1 3.9 -4.0 52 54 A G E -DE 30 61A 1 -22,-1.1 -22,-2.3 -2,-0.3 2,-0.3 -0.994 48.8 -80.8 160.5-155.5 -14.2 1.6 -6.2 53 55 A W E -DE 29 60A 9 7,-1.9 7,-2.5 -2,-0.3 2,-0.4 -0.991 15.1-153.2-146.6 150.1 -14.3 -0.3 -9.5 54 56 A V E + E 0 59A 12 -26,-2.2 2,-0.3 -2,-0.3 5,-0.2 -0.991 12.2 176.3-124.2 135.5 -14.9 0.2 -13.2 55 57 A F E > - E 0 58A 19 3,-2.9 3,-2.4 -2,-0.4 2,-0.5 -0.831 67.5 -62.5-138.3 91.6 -16.1 -2.6 -15.5 56 58 A G T 3 S- 0 0 51 -2,-0.3 -51,-0.1 1,-0.3 138,-0.0 -0.519 122.3 -14.9 69.2-113.3 -16.7 -1.3 -19.0 57 59 A T T 3 S+ 0 0 40 -2,-0.5 25,-2.5 -3,-0.1 2,-0.3 0.475 119.8 96.3 -97.7 -5.1 -19.4 1.3 -18.5 58 60 A A E < +EF 55 81A 0 -3,-2.4 -3,-2.9 23,-0.2 2,-0.3 -0.657 52.1 176.1 -82.4 144.1 -20.4 0.1 -15.0 59 61 A I E -EF 54 80A 0 21,-2.7 21,-2.8 -2,-0.3 2,-0.4 -0.996 18.1-172.2-148.0 140.4 -18.8 1.9 -12.0 60 62 A S E +EF 53 79A 3 -7,-2.5 -7,-1.9 -2,-0.3 2,-0.3 -0.985 12.3 173.1-122.0 145.2 -18.9 1.9 -8.3 61 63 A F E -EF 52 78A 2 17,-2.0 17,-3.3 -2,-0.4 2,-0.3 -0.990 7.1-170.4-147.6 151.1 -17.3 4.4 -6.0 62 64 A S E -EF 51 77A 8 -11,-2.5 -11,-1.9 -2,-0.3 2,-0.4 -0.998 4.4-161.4-143.4 142.0 -17.4 5.0 -2.3 63 65 A T E -EF 50 76A 0 13,-2.4 13,-2.2 -2,-0.3 2,-0.5 -0.976 14.0-142.7-127.0 138.0 -16.2 7.7 0.1 64 66 A K E - F 0 75A 18 -15,-0.9 2,-2.2 -2,-0.4 11,-0.2 -0.875 23.5-132.8 -92.9 124.3 -15.6 7.7 3.8 65 67 A W E S+ 0 0 19 9,-2.5 7,-2.9 -2,-0.5 8,-0.7 -0.323 72.7 100.2 -82.6 61.6 -16.6 11.1 5.3 66 68 A L E + F 0 71A 71 -2,-2.2 2,-0.2 5,-0.2 5,-0.2 -0.983 36.9 141.6-141.2 129.0 -13.5 11.5 7.4 67 69 A N - 0 0 33 3,-1.0 -19,-0.1 -2,-0.4 -2,-0.0 -0.814 65.8 -75.9-146.0-166.8 -10.5 13.7 6.5 68 70 A S S S+ 0 0 123 -2,-0.2 3,-0.1 1,-0.2 -2,-0.0 0.511 125.4 35.9 -77.1 -5.0 -8.1 16.0 8.4 69 71 A V S S+ 0 0 108 1,-0.3 2,-0.3 -26,-0.0 -1,-0.2 0.670 120.8 10.7-118.7 -26.4 -10.6 18.8 8.6 70 72 A E - 0 0 74 -27,-0.1 -3,-1.0 -28,-0.0 2,-0.4 -0.987 50.6-153.1-161.6 140.1 -14.1 17.4 9.2 71 73 A S E -F 66 0A 59 -2,-0.3 -5,-0.2 -5,-0.2 -28,-0.2 -0.958 10.7-179.6-112.2 138.6 -16.0 14.2 9.9 72 74 A a E - 0 0 37 -7,-2.9 -6,-0.1 -2,-0.4 3,-0.1 0.206 42.4-129.2-117.3 14.6 -19.6 13.3 9.0 73 75 A N E S+ 0 0 65 -8,-0.7 27,-2.8 1,-0.2 2,-0.3 0.785 73.1 82.3 57.0 35.7 -19.5 9.9 10.7 74 76 A S E - G 0 99A 10 25,-0.3 -9,-2.5 -9,-0.2 2,-0.4 -0.921 66.2-127.6-161.3 173.4 -20.7 8.0 7.6 75 77 A I E -FG 64 98A 0 23,-2.2 23,-2.5 -2,-0.3 2,-0.4 -0.999 15.2-155.9-137.2 131.3 -19.9 6.4 4.2 76 78 A T E -FG 63 97A 9 -13,-2.2 -13,-2.4 -2,-0.4 2,-0.4 -0.917 7.8-168.0-109.0 140.3 -21.6 7.0 0.9 77 79 A S E -FG 62 96A 6 19,-2.7 19,-2.3 -2,-0.4 2,-0.4 -0.995 2.6-162.5-127.2 129.5 -21.5 4.4 -1.9 78 80 A W E -FG 61 95A 5 -17,-3.3 -17,-2.0 -2,-0.4 2,-0.4 -0.896 4.9-170.0-109.9 140.7 -22.6 5.1 -5.5 79 81 A S E +FG 60 94A 0 15,-2.7 15,-2.5 -2,-0.4 2,-0.3 -0.998 30.3 133.9-123.7 131.3 -23.4 2.5 -8.2 80 82 A G E -FG 59 93A 0 -21,-2.8 -21,-2.7 -2,-0.4 2,-0.3 -0.937 41.3-119.5-164.2-176.8 -23.8 3.7 -11.7 81 83 A F E -FG 58 92A 4 11,-2.1 11,-2.6 -2,-0.3 2,-0.3 -0.988 26.3-114.7-141.1 150.6 -23.3 3.5 -15.4 82 84 A Y E + G 0 91A 31 -25,-2.5 2,-0.3 -2,-0.3 9,-0.2 -0.557 30.5 178.6 -83.7 139.3 -21.7 5.6 -18.0 83 85 A I E - 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