==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=29-JUL-2012 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 28-JUL-11 3T6H . COMPND 2 MOLECULE: TAGRFP-T; . SOURCE 2 ORGANISM_SCIENTIFIC: SYNTHETIC CONSTRUCT; . AUTHOR X.HU,R.LIU . 900 8 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 33246.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 646 71.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 53 5.9 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 408 45.3 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 12 1.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 69 7.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 84 9.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 29 3.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 11 1.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 4 0 0 0 0 0 0 3 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 4 0 0 8 0 4 4 8 4 12 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 A G > 0 0 51 0, 0.0 3,-1.4 0, 0.0 4,-0.4 0.000 360.0 360.0 360.0 -69.7 -32.5 28.2 -12.8 2 2 A E T 3 + 0 0 78 1,-0.2 3,-0.3 2,-0.2 78,-0.1 0.576 360.0 72.9 -61.0 -12.7 -29.4 27.9 -14.9 3 3 A E T 3 S+ 0 0 150 1,-0.2 -1,-0.2 79,-0.1 77,-0.1 0.686 86.0 62.8 -74.5 -22.4 -27.7 29.5 -11.7 4 4 A L S < S+ 0 0 54 -3,-1.4 2,-0.8 75,-0.2 -1,-0.2 0.754 87.6 79.5 -77.2 -22.3 -28.1 26.2 -9.9 5 5 A I S S- 0 0 4 -4,-0.4 -1,-0.1 -3,-0.3 4,-0.1 -0.792 74.8-161.7 -88.7 108.1 -25.8 24.5 -12.4 6 6 A K - 0 0 113 -2,-0.8 29,-0.2 2,-0.2 3,-0.1 -0.180 33.6-101.7 -85.6 176.9 -22.3 25.4 -11.4 7 7 A E S S+ 0 0 93 1,-0.2 2,-0.4 26,-0.1 29,-0.3 0.704 114.1 38.4 -77.1 -20.1 -19.1 25.3 -13.5 8 8 A N S S+ 0 0 104 25,-0.2 2,-0.3 26,-0.1 25,-0.2 -1.000 78.0 177.7-130.0 126.0 -18.1 22.0 -11.8 9 9 A M E -A 32 0A 9 23,-2.7 23,-2.0 -2,-0.4 2,-0.2 -0.968 19.5-147.6-134.8 148.5 -20.8 19.4 -10.8 10 10 A H E -A 31 0A 122 101,-0.3 103,-2.1 -2,-0.3 2,-0.3 -0.700 16.6-166.3-103.1 160.2 -21.0 15.9 -9.3 11 11 A M E -Ab 30 113A 1 19,-2.0 19,-2.0 -2,-0.2 2,-0.3 -0.993 9.3-163.8-146.0 152.8 -23.6 13.3 -10.2 12 12 A K E -Ab 29 114A 104 101,-2.5 103,-2.6 -2,-0.3 2,-0.4 -0.975 15.7-167.0-133.7 149.1 -24.7 10.0 -8.7 13 13 A L E -Ab 28 115A 10 15,-2.4 15,-2.0 -2,-0.3 2,-0.4 -0.994 18.4-164.5-144.9 141.0 -26.8 7.3 -10.5 14 14 A Y E -Ab 27 116A 85 101,-2.6 103,-2.7 -2,-0.4 2,-0.4 -0.993 14.7-171.2-122.2 131.7 -28.8 4.2 -10.0 15 15 A M E +Ab 26 117A 0 11,-3.0 11,-2.5 -2,-0.4 2,-0.4 -0.976 7.9 177.9-121.8 135.5 -29.7 1.9 -12.9 16 16 A E E +Ab 25 118A 61 101,-2.2 103,-1.8 -2,-0.4 2,-0.3 -0.988 26.4 126.0-129.3 134.0 -32.1 -1.1 -12.9 17 17 A G E -Ab 24 119A 2 7,-2.5 7,-2.3 -2,-0.4 2,-0.3 -0.988 42.0-125.1-166.5-178.5 -32.8 -3.1 -16.0 18 18 A T E -Ab 23 120A 10 101,-1.5 103,-3.2 -2,-0.3 2,-0.4 -0.999 8.7-169.9-138.4 147.9 -33.1 -6.3 -18.2 19 19 A V E > S-Ab 22 121A 1 3,-1.6 3,-2.3 -2,-0.3 103,-0.2 -0.976 80.5 -23.9-134.2 122.4 -31.7 -7.3 -21.5 20 20 A N T 3 S- 0 0 7 101,-2.3 102,-0.1 -2,-0.4 -1,-0.1 0.891 129.4 -45.5 46.9 52.8 -33.1 -10.5 -23.1 21 21 A N T 3 S+ 0 0 86 100,-0.4 2,-0.7 1,-0.2 -1,-0.3 0.494 109.9 121.7 68.9 1.9 -34.2 -11.9 -19.7 22 22 A H E < -A 19 0A 59 -3,-2.3 -3,-1.6 2,-0.0 -1,-0.2 -0.887 51.0-151.6 -95.2 112.3 -31.0 -11.1 -17.8 23 23 A H E +A 18 0A 64 -2,-0.7 2,-0.3 -5,-0.2 -5,-0.2 -0.489 23.4 164.0 -79.6 143.1 -31.7 -8.7 -14.8 24 24 A F E -A 17 0A 0 -7,-2.3 -7,-2.5 -2,-0.2 2,-0.3 -0.985 24.7-154.4-152.1 158.3 -29.0 -6.3 -13.6 25 25 A K E -AC 16 47A 42 22,-1.9 21,-1.8 -2,-0.3 22,-1.6 -0.981 14.5-174.2-131.5 148.0 -28.5 -3.2 -11.4 26 26 A C E -AC 15 45A 0 -11,-2.5 -11,-3.0 -2,-0.3 2,-0.3 -0.947 6.9-166.2-131.4 158.2 -25.7 -0.6 -11.7 27 27 A T E +AC 14 44A 47 17,-2.6 17,-3.2 -2,-0.3 2,-0.3 -0.965 13.2 174.7-134.9 158.4 -24.6 2.4 -9.7 28 28 A S E -AC 13 43A 4 -15,-2.0 -15,-2.4 -2,-0.3 2,-0.3 -0.997 16.7-165.4-157.9 162.5 -22.2 5.1 -10.6 29 29 A E E +AC 12 42A 114 13,-1.8 13,-2.7 -2,-0.3 2,-0.3 -0.978 25.0 139.0-141.4 150.3 -20.7 8.4 -9.6 30 30 A G E -AC 11 41A 2 -19,-2.0 -19,-2.0 -2,-0.3 2,-0.3 -0.944 29.6-128.9-165.6-161.9 -18.8 11.0 -11.6 31 31 A E E +AC 10 40A 71 9,-1.6 9,-2.5 -2,-0.3 2,-0.3 -0.980 22.1 156.6-160.2 154.0 -18.1 14.7 -12.3 32 32 A G E -AC 9 39A 10 -23,-2.0 -23,-2.7 -2,-0.3 7,-0.3 -0.969 43.8 -90.8-163.3-169.4 -17.8 17.2 -15.1 33 33 A K E >> - C 0 38A 79 5,-2.0 5,-1.5 -2,-0.3 4,-1.2 -0.936 29.9-162.8-117.5 97.9 -17.9 20.7 -16.4 34 34 A P T 45S+ 0 0 0 0, 0.0 34,-0.2 0, 0.0 3,-0.2 0.815 89.7 41.8 -55.2 -44.9 -21.5 21.5 -17.5 35 35 A Y T 45S+ 0 0 63 -28,-0.2 -28,-0.1 1,-0.2 -27,-0.1 0.696 114.5 53.5 -76.9 -22.1 -20.6 24.5 -19.7 36 36 A E T 45S- 0 0 97 -3,-0.3 33,-0.3 -29,-0.3 -1,-0.2 0.633 105.2-129.1 -85.6 -11.7 -17.6 22.8 -21.1 37 37 A G T <5 + 0 0 1 -4,-1.2 178,-2.2 31,-0.3 2,-0.3 0.768 65.9 119.7 73.9 26.2 -19.5 19.7 -22.2 38 38 A T E < +CD 33 214A 36 -5,-1.5 -5,-2.0 176,-0.2 2,-0.3 -0.938 28.3 155.0-127.3 146.0 -17.2 17.2 -20.5 39 39 A Q E -CD 32 213A 9 174,-1.6 174,-2.4 -2,-0.3 2,-0.3 -0.962 23.7-150.7-163.0 161.3 -17.8 14.6 -17.8 40 40 A T E -CD 31 212A 28 -9,-2.5 -9,-1.6 -2,-0.3 2,-0.4 -0.958 12.2-165.6-133.7 138.9 -16.5 11.2 -16.5 41 41 A M E -CD 30 211A 6 170,-3.0 170,-2.1 -2,-0.3 2,-0.5 -0.990 11.6-151.3-129.0 144.5 -18.5 8.5 -14.8 42 42 A R E -CD 29 210A 81 -13,-2.7 -13,-1.8 -2,-0.4 2,-0.4 -0.977 25.3-161.2-113.7 121.0 -17.2 5.6 -12.8 43 43 A I E -CD 28 209A 1 166,-3.6 166,-2.0 -2,-0.5 2,-0.4 -0.863 13.7-168.1-115.8 137.5 -19.7 2.7 -13.1 44 44 A K E -CD 27 208A 128 -17,-3.2 -17,-2.6 -2,-0.4 2,-0.7 -0.980 17.6-145.2-122.5 134.1 -20.3 -0.5 -11.0 45 45 A V E +C 26 0A 18 162,-1.7 -19,-0.3 -2,-0.4 3,-0.1 -0.866 25.7 174.1 -87.6 115.6 -22.5 -3.4 -11.9 46 46 A V E + 0 0 82 -21,-1.8 2,-0.4 -2,-0.7 -20,-0.2 0.644 60.5 31.3-105.8 -18.2 -23.7 -4.3 -8.4 47 47 A E E S+C 25 0A 96 -22,-1.6 -22,-1.9 1,-0.1 -1,-0.3 -0.996 117.9 13.2-143.3 131.6 -26.2 -7.1 -9.3 48 48 A G S S+ 0 0 30 -2,-0.4 -2,-0.1 1,-0.3 -1,-0.1 0.551 90.0 173.6 87.5 11.2 -26.3 -9.6 -12.1 49 49 A G + 0 0 38 2,-0.1 -1,-0.3 -24,-0.1 2,-0.1 -0.567 50.7 61.6 -94.9 163.4 -22.7 -9.2 -13.3 50 50 A P S S- 0 0 73 0, 0.0 156,-0.1 0, 0.0 155,-0.1 0.502 88.8-136.6 -68.8 136.9 -20.6 -10.2 -15.1 51 51 A L - 0 0 8 154,-0.4 4,-0.1 1,-0.1 -2,-0.1 -0.369 4.6-141.3 -63.9 139.7 -23.1 -9.3 -17.8 52 52 A P S S+ 0 0 51 0, 0.0 2,-0.3 0, 0.0 -1,-0.1 0.401 82.2 58.7 -80.7 4.2 -23.6 -11.7 -20.7 53 53 A F S S- 0 0 0 79,-0.1 78,-0.2 80,-0.1 77,-0.1 -0.900 102.4 -76.4-129.4 160.0 -23.9 -8.9 -23.3 54 54 A A > - 0 0 1 76,-2.4 3,-1.4 -2,-0.3 4,-0.2 -0.291 31.7-142.7 -48.8 122.2 -21.6 -6.0 -24.4 55 55 A F G >> S+ 0 0 0 1,-0.2 3,-2.4 2,-0.1 4,-0.6 0.799 92.1 83.9 -60.8 -26.3 -21.6 -3.1 -21.8 56 56 A D G >4 S+ 0 0 7 1,-0.3 3,-1.0 2,-0.2 4,-0.5 0.782 78.9 61.2 -41.6 -36.5 -21.5 -0.9 -24.8 57 57 A I G <4 S+ 0 0 3 -3,-1.4 -1,-0.3 1,-0.3 -2,-0.1 0.646 107.8 44.9 -77.7 -12.3 -25.3 -1.0 -25.3 58 58 A L G X> S+ 0 0 0 -3,-2.4 3,-1.7 -4,-0.2 4,-0.6 0.506 88.1 94.5 -98.2 -7.4 -25.8 0.5 -21.9 59 59 A A G X< S+ 0 0 2 -3,-1.0 3,-1.2 -4,-0.6 -2,-0.2 0.883 83.0 48.3 -61.5 -44.8 -23.1 3.2 -22.4 60 60 A T G 34 S+ 0 0 26 -4,-0.5 -1,-0.3 1,-0.2 -2,-0.1 0.503 100.6 66.4 -71.6 -6.3 -25.5 5.9 -23.6 61 61 A S G <4 0 0 1 -3,-1.7 -1,-0.2 1,-0.1 -2,-0.2 0.676 360.0 360.0 -90.0 -11.5 -27.9 5.4 -20.8 62 62 A F << 0 0 12 -3,-1.2 -2,-0.2 -4,-0.6 -1,-0.1 0.870 360.0 360.0 -82.5 360.0 -25.4 6.6 -18.2 63 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 64 66 A S > 0 0 12 0, 0.0 3,-1.0 0, 0.0 -2,-0.1 0.000 360.0 360.0 360.0 78.9 -26.6 13.0 -18.7 65 67 A R T 3 + 0 0 14 1,-0.2 3,-0.4 2,-0.0 13,-0.2 0.480 360.0 77.5 -77.7 -10.8 -27.8 16.1 -20.6 66 68 A T T 3 S+ 0 0 0 1,-0.2 2,-1.2 12,-0.1 -1,-0.2 0.820 86.4 68.7 -67.6 -22.6 -26.8 18.9 -18.2 67 69 A F < + 0 0 0 -3,-1.0 2,-0.4 -30,-0.1 -1,-0.2 -0.411 69.9 117.1-102.3 56.5 -23.2 18.5 -19.4 68 70 A I - 0 0 2 -2,-1.2 2,-0.9 -3,-0.4 -31,-0.3 -0.985 63.5-127.1-122.1 138.5 -23.5 19.8 -23.0 69 71 A N E -e 216 0A 48 146,-2.8 148,-3.0 -2,-0.4 2,-1.0 -0.749 21.4-156.2 -87.9 105.4 -21.6 22.9 -24.2 70 72 A H E -e 217 0A 32 -2,-0.9 3,-0.4 146,-0.2 2,-0.2 -0.775 17.4-175.8 -86.2 102.7 -24.2 25.2 -25.8 71 73 A T > + 0 0 41 146,-2.0 3,-1.6 -2,-1.0 145,-0.0 -0.462 57.2 31.5 -90.3 166.0 -22.3 27.4 -28.2 72 74 A Q T 3 S- 0 0 101 1,-0.3 -1,-0.2 -2,-0.2 146,-0.1 0.717 127.1 -67.6 61.3 26.7 -23.7 30.3 -30.3 73 75 A G T 3 S+ 0 0 68 -3,-0.4 -1,-0.3 1,-0.2 -2,-0.1 0.674 76.9 168.6 74.0 17.5 -26.3 31.3 -27.8 74 76 A I < - 0 0 6 -3,-1.6 -1,-0.2 143,-0.2 143,-0.1 -0.564 43.5-113.2 -64.2 118.8 -28.4 28.1 -28.1 75 77 A P - 0 0 21 0, 0.0 2,-1.0 0, 0.0 -1,-0.1 -0.267 27.5-125.7 -52.4 132.5 -30.9 28.2 -25.2 76 78 A D > - 0 0 6 1,-0.2 4,-2.3 -6,-0.1 5,-0.1 -0.712 26.1-175.0 -94.3 103.4 -30.1 25.3 -22.8 77 79 A F T 4 S+ 0 0 0 -2,-1.0 4,-0.4 1,-0.2 -1,-0.2 0.823 84.6 43.5 -62.6 -37.5 -33.3 23.3 -22.3 78 80 A F T >4 S+ 0 0 0 -13,-0.2 3,-1.0 2,-0.2 4,-0.3 0.928 114.0 47.2 -81.8 -46.2 -31.7 21.0 -19.8 79 81 A K G >4 S+ 0 0 1 1,-0.3 3,-1.2 -14,-0.2 -2,-0.2 0.901 110.6 53.8 -63.7 -37.6 -29.9 23.6 -17.7 80 82 A Q G 3< S+ 0 0 32 -4,-2.3 -1,-0.3 1,-0.2 -2,-0.2 0.609 93.7 74.8 -68.6 -17.3 -33.0 25.9 -17.6 81 83 A S G <> S+ 0 0 0 -3,-1.0 4,-2.9 -4,-0.4 -1,-0.2 0.616 78.9 138.1 -72.0 -13.4 -35.0 23.0 -16.2 82 84 A F T <4 + 0 0 0 -3,-1.2 -79,-0.1 -4,-0.3 -78,-0.1 -0.439 52.1 23.2 -82.3 154.4 -33.6 23.1 -12.7 83 85 A P T 4 S+ 0 0 72 0, 0.0 25,-0.6 0, 0.0 -1,-0.3 -0.993 132.3 42.6 -75.4 -22.3 -34.5 23.0 -9.8 84 86 A E T 4 S- 0 0 88 -3,-0.2 -2,-0.3 1,-0.1 2,-0.2 0.926 99.4-161.7 -54.6 -44.9 -37.5 21.0 -11.0 85 87 A G < - 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