==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=5-JAN-2010 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 20-SEP-08 2V4E . COMPND 2 MOLECULE: RED FLUORESCENT PROTEIN DRFP583; . SOURCE 2 ORGANISM_SCIENTIFIC: DISCOSOMA SP.; . AUTHOR R.L.STRACK,D.E.STRONGIN,D.BHATTACHARYYA,W.TAO,A.BERMAN, . 1732 16 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 64438.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 1237 71.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 101 5.8 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 813 46.9 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 16 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 16 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 24 1.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 116 6.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 135 7.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 56 3.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 13 0.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 4 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 9 0 0 0 0 0 0 0 5 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 8 0 0 9 7 8 9 15 17 15 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 7 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 8 A I 0 0 49 0, 0.0 4,-0.1 0, 0.0 74,-0.0 0.000 360.0 360.0 360.0 120.4 59.1 -9.0 -60.3 2 9 A K - 0 0 138 105,-0.1 29,-0.1 1,-0.1 3,-0.1 -0.525 360.0-103.0-105.1 163.4 59.6 -5.6 -58.8 3 10 A P S S+ 0 0 96 0, 0.0 2,-0.3 0, 0.0 28,-0.2 0.574 111.8 22.4 -70.2 -8.9 58.1 -3.9 -55.8 4 11 A F S S+ 0 0 98 25,-0.2 2,-0.3 26,-0.1 25,-0.2 -0.889 75.0 168.1-154.2 128.7 55.9 -1.9 -58.1 5 12 A M E -A 28 0A 8 23,-2.0 23,-2.0 -2,-0.3 2,-0.3 -0.968 24.1-133.5-139.1 155.2 54.9 -2.8 -61.7 6 13 A R E -A 27 0A 123 101,-0.5 103,-2.2 -2,-0.3 2,-0.3 -0.738 18.5-160.9-101.3 153.8 52.5 -1.6 -64.2 7 14 A F E -Ab 26 109A 1 19,-3.5 19,-2.5 -2,-0.3 2,-0.4 -0.953 7.7-152.1-131.2 155.8 50.2 -3.7 -66.4 8 15 A K E -Ab 25 110A 115 101,-3.0 103,-3.7 -2,-0.3 2,-0.4 -0.981 16.0-163.9-122.8 142.2 48.3 -3.2 -69.6 9 16 A V E -Ab 24 111A 4 15,-3.1 15,-2.1 -2,-0.4 2,-0.5 -0.983 14.3-172.4-136.0 135.0 45.1 -5.1 -70.5 10 17 A H E -Ab 23 112A 47 101,-2.5 103,-3.0 -2,-0.4 2,-0.4 -0.986 14.6-168.4-120.5 123.4 43.0 -5.8 -73.6 11 18 A M E -Ab 22 113A 0 11,-3.0 11,-2.4 -2,-0.5 2,-0.5 -0.961 12.9-174.2-117.5 133.6 39.6 -7.5 -73.3 12 19 A E E +Ab 21 114A 55 101,-2.5 103,-1.9 -2,-0.4 2,-0.3 -0.990 28.0 162.1-118.3 120.9 37.4 -9.0 -76.0 13 20 A G E -Ab 20 115A 6 7,-2.5 7,-2.1 -2,-0.5 2,-0.3 -0.898 30.2-156.0-137.5 164.0 34.1 -10.2 -74.6 14 21 A S E -Ab 19 116A 2 101,-2.1 103,-3.2 -2,-0.3 2,-0.4 -0.997 6.3-177.0-137.5 137.7 30.5 -11.2 -75.3 15 22 A V E > S-Ab 18 117A 0 3,-2.3 3,-2.9 -2,-0.3 103,-0.2 -0.992 78.0 -18.8-130.9 131.6 27.4 -11.2 -73.1 16 23 A N T 3 S- 0 0 43 101,-1.3 102,-0.1 -2,-0.4 -1,-0.1 0.880 129.0 -50.0 40.9 52.6 24.1 -12.5 -74.4 17 24 A G T 3 S+ 0 0 8 100,-0.4 2,-0.7 1,-0.2 -1,-0.3 0.439 113.6 119.7 71.8 -2.2 25.3 -12.2 -78.0 18 25 A H E < -A 15 0A 70 -3,-2.9 -3,-2.3 2,-0.0 -1,-0.2 -0.877 50.1-155.2-101.1 116.8 26.4 -8.6 -77.5 19 26 A E E +A 14 0A 96 -2,-0.7 2,-0.3 -5,-0.2 -5,-0.2 -0.435 18.0 165.9 -89.6 158.8 30.1 -8.1 -78.1 20 27 A F E -A 13 0A 6 -7,-2.1 -7,-2.5 -2,-0.1 2,-0.3 -0.976 23.8-139.3-164.7 159.1 32.4 -5.4 -76.7 21 28 A E E -AC 12 43A 47 22,-2.3 22,-1.7 -2,-0.3 21,-1.1 -0.972 15.8-167.8-128.3 146.0 36.1 -4.4 -76.3 22 29 A I E -AC 11 41A 0 -11,-2.4 -11,-3.0 -2,-0.3 2,-0.4 -0.990 3.2-165.3-137.9 143.2 37.8 -2.9 -73.3 23 30 A E E +AC 10 40A 75 17,-2.8 17,-3.7 -2,-0.4 2,-0.3 -0.967 20.3 150.9-129.2 143.8 41.2 -1.3 -72.8 24 31 A G E -AC 9 39A 13 -15,-2.1 -15,-3.1 -2,-0.4 2,-0.3 -0.999 29.4-145.8-166.4 164.9 43.0 -0.5 -69.6 25 32 A E E +AC 8 38A 94 13,-1.8 13,-3.2 -2,-0.3 2,-0.3 -0.938 20.9 175.1-132.7 155.2 46.1 0.0 -67.5 26 33 A G E -AC 7 37A 1 -19,-2.5 -19,-3.5 -2,-0.3 2,-0.3 -0.976 10.3-161.3-155.8 172.2 46.9 -0.7 -63.9 27 34 A E E +AC 6 36A 73 9,-1.6 9,-2.5 -2,-0.3 2,-0.3 -0.996 19.1 132.2-155.8 146.9 49.6 -0.6 -61.3 28 35 A G E -AC 5 35A 3 -23,-2.0 -23,-2.0 -2,-0.3 7,-0.2 -0.979 53.6 -71.8-172.9-165.1 50.5 -2.0 -57.8 29 36 A K E >>> - C 0 34A 85 5,-1.5 3,-1.8 -2,-0.3 4,-1.6 -0.849 32.4-162.0-111.4 94.4 52.8 -3.7 -55.4 30 37 A P T 345S+ 0 0 0 0, 0.0 34,-0.4 0, 0.0 -1,-0.1 0.690 90.8 43.8 -53.4 -25.3 53.1 -7.4 -56.3 31 38 A Y T 345S+ 0 0 119 -28,-0.2 35,-0.2 1,-0.1 32,-0.1 0.569 115.1 46.8 -96.5 -15.5 54.5 -8.3 -52.8 32 39 A E T <45S- 0 0 111 -3,-1.8 33,-0.2 2,-0.2 176,-0.2 0.470 104.4-130.5-101.1 -8.0 52.0 -6.2 -50.8 33 40 A G T <5 + 0 0 0 -4,-1.6 175,-3.2 1,-0.2 2,-0.3 0.812 63.2 122.0 65.6 33.2 49.1 -7.5 -52.9 34 41 A T E < +CD 29 207A 32 -5,-0.8 -5,-1.5 173,-0.2 2,-0.3 -0.901 28.4 154.7-118.5 154.1 47.4 -4.2 -53.7 35 42 A Q E -CD 28 206A 11 171,-1.0 171,-2.4 -2,-0.3 2,-0.3 -0.956 23.7-151.4-168.4 157.7 46.7 -2.8 -57.1 36 43 A T E -CD 27 205A 55 -9,-2.5 -9,-1.6 -2,-0.3 2,-0.3 -0.854 11.3-168.0-127.6 169.6 44.5 -0.4 -59.1 37 44 A A E -CD 26 204A 8 167,-1.7 167,-1.9 -2,-0.3 2,-0.4 -0.986 14.0-149.5-154.7 148.6 43.3 -0.3 -62.7 38 45 A K E -CD 25 203A 78 -13,-3.2 -13,-1.8 -2,-0.3 2,-0.4 -0.994 21.1-168.8-123.5 124.7 41.6 2.1 -65.1 39 46 A L E -CD 24 202A 0 163,-2.8 163,-2.1 -2,-0.4 2,-0.4 -0.923 8.0-166.9-118.1 137.0 39.4 0.6 -67.9 40 47 A K E -CD 23 201A 95 -17,-3.7 -17,-2.8 -2,-0.4 2,-1.0 -0.977 24.1-130.3-116.7 136.3 37.7 2.2 -71.0 41 48 A V E +C 22 0A 20 159,-2.6 -19,-0.2 -2,-0.4 4,-0.1 -0.734 30.5 174.4 -83.1 107.1 35.1 0.6 -73.2 42 49 A T E + 0 0 72 -21,-1.1 2,-0.3 -2,-1.0 -20,-0.2 0.575 63.3 25.3 -89.5 -12.6 36.6 1.1 -76.6 43 50 A K E S+C 21 0A 129 -22,-1.7 -22,-2.3 -3,-0.1 -1,-0.2 -0.944 116.2 13.7-159.9 129.8 33.9 -0.9 -78.5 44 51 A G S S- 0 0 30 -2,-0.3 -2,-0.1 1,-0.3 -1,-0.0 0.686 90.5-178.1 83.7 20.9 30.2 -1.9 -78.1 45 52 A G + 0 0 39 -4,-0.1 -1,-0.3 2,-0.1 2,-0.1 -0.554 52.8 60.4 -97.5 164.6 29.4 0.6 -75.2 46 53 A P S S- 0 0 75 0, 0.0 153,-0.1 0, 0.0 154,-0.1 0.521 89.9-128.2 -67.0 139.1 27.5 1.6 -73.3 47 54 A L - 0 0 9 151,-0.4 -2,-0.1 1,-0.1 4,-0.1 -0.385 8.7-147.1 -63.0 131.3 27.5 -2.0 -72.0 48 55 A P S S+ 0 0 43 0, 0.0 2,-0.3 0, 0.0 -1,-0.1 0.339 77.1 57.6 -84.3 10.2 23.9 -3.2 -71.7 49 56 A F S S- 0 0 4 79,-0.1 78,-0.2 80,-0.1 2,-0.2 -0.834 100.6 -70.2-134.7 171.9 24.8 -5.4 -68.7 50 57 A A > - 0 0 6 76,-2.3 3,-1.1 -2,-0.3 4,-0.3 -0.415 31.8-143.4 -74.9 132.0 26.2 -5.0 -65.2 51 58 A W G >> S+ 0 0 9 1,-0.2 3,-2.5 -2,-0.2 4,-1.1 0.808 88.7 79.7 -61.7 -33.7 29.9 -4.1 -65.0 52 59 A D G 34 S+ 0 0 13 1,-0.3 -1,-0.2 2,-0.2 3,-0.2 0.772 84.9 59.6 -51.0 -32.2 30.5 -6.2 -61.9 53 60 A I G <4 S+ 0 0 0 -3,-1.1 -1,-0.3 1,-0.2 -2,-0.2 0.761 109.8 45.2 -68.3 -22.2 30.7 -9.5 -63.9 54 61 A L T X> S+ 0 0 0 -3,-2.5 3,-2.1 -4,-0.3 4,-0.6 0.760 92.5 81.8 -85.8 -26.6 33.6 -8.0 -65.8 55 62 A S G >< S+ 0 0 1 -4,-1.1 3,-0.6 1,-0.3 -2,-0.1 0.765 87.4 51.8 -64.2 -33.4 35.6 -6.6 -62.9 56 63 A P G 34 S+ 0 0 21 0, 0.0 -1,-0.3 0, 0.0 -2,-0.1 0.399 98.2 67.5 -82.1 5.2 37.4 -9.7 -61.7 57 64 A Q G <4 0 0 1 -3,-2.1 -2,-0.2 1,-0.1 -3,-0.1 0.743 360.0 360.0 -98.1 -3.4 38.7 -10.4 -65.2 58 65 A F << 0 0 11 -4,-0.6 -1,-0.1 -3,-0.6 -3,-0.1 0.867 360.0 360.0 -95.4 360.0 40.9 -7.3 -65.1 59 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 60 69 A S > 0 0 11 0, 0.0 3,-1.6 0, 0.0 -2,-0.1 0.000 360.0 360.0 360.0 48.9 45.9 -9.9 -61.9 61 70 A K T 3 + 0 0 27 1,-0.3 3,-0.3 2,-0.1 13,-0.2 0.311 360.0 84.6 -80.3 8.3 46.9 -12.4 -59.2 62 71 A V T 3 S+ 0 0 0 1,-0.2 2,-2.5 12,-0.1 -1,-0.3 0.896 83.2 66.7 -66.8 -38.7 50.6 -11.8 -59.7 63 72 A Y S < S+ 0 0 0 -3,-1.6 2,-0.4 -30,-0.1 -1,-0.2 -0.307 78.0 121.1 -75.7 58.6 49.9 -8.9 -57.4 64 73 A T - 0 0 1 -2,-2.5 2,-0.3 -34,-0.4 145,-0.2 -0.990 64.9-121.0-127.1 134.2 49.1 -11.2 -54.4 65 74 A K B -e 209 0A 93 143,-1.8 145,-3.5 -2,-0.4 -34,-0.1 -0.504 31.6-170.1 -71.2 124.7 50.9 -11.2 -51.1 66 75 A H - 0 0 31 -2,-0.3 145,-0.1 143,-0.2 6,-0.1 -0.947 21.8-126.9-118.1 131.1 52.5 -14.5 -50.3 67 76 A P > - 0 0 48 0, 0.0 3,-1.1 0, 0.0 144,-0.0 -0.373 37.4-107.1 -65.6 163.3 54.1 -15.6 -47.0 68 77 A A T 3 S+ 0 0 117 1,-0.2 4,-0.1 3,-0.0 -2,-0.0 0.711 115.6 57.1 -71.1 -19.8 57.6 -17.0 -47.6 69 78 A D T 3 S+ 0 0 110 1,-0.1 -1,-0.2 2,-0.1 -3,-0.0 0.565 92.3 75.3 -89.8 -8.9 56.6 -20.6 -46.9 70 79 A I S < S- 0 0 9 -3,-1.1 -1,-0.1 111,-0.1 140,-0.1 -0.899 90.5-120.8-103.7 110.5 53.9 -20.7 -49.7 71 80 A P - 0 0 32 0, 0.0 2,-1.3 0, 0.0 3,-0.2 -0.205 16.0-134.3 -48.6 129.6 55.5 -21.0 -53.2 72 81 A D > + 0 0 22 1,-0.2 4,-3.0 -4,-0.1 5,-0.2 -0.686 29.3 177.6 -90.1 82.4 54.4 -18.0 -55.4 73 82 A Y H > S+ 0 0 8 -2,-1.3 4,-1.1 1,-0.2 -1,-0.2 0.859 76.1 48.4 -54.6 -45.7 53.6 -20.0 -58.5 74 83 A K H 4 S+ 0 0 4 2,-0.2 4,-0.3 1,-0.2 -1,-0.2 0.891 114.2 44.2 -68.3 -42.6 52.4 -17.0 -60.5 75 84 A K H >4 S+ 0 0 19 1,-0.2 3,-1.8 2,-0.2 -2,-0.2 0.935 110.5 55.6 -65.9 -45.2 55.4 -14.8 -59.7 76 85 A L H 3< S+ 0 0 85 -4,-3.0 -1,-0.2 1,-0.3 -2,-0.2 0.795 94.4 71.8 -57.2 -27.9 57.9 -17.7 -60.3 77 86 A S T 3X S+ 0 0 0 -4,-1.1 4,-3.0 -5,-0.2 -1,-0.3 0.663 87.1 138.4 -66.3 -16.8 56.4 -18.1 -63.8 78 87 A F T <4 + 0 0 14 -3,-1.8 25,-0.0 2,-0.3 96,-0.0 -0.379 45.1 24.7 -84.7 162.0 58.0 -14.9 -65.1 79 88 A P T 4 S+ 0 0 80 0, 0.0 25,-1.2 0, 0.0 -1,-0.3 -0.973 134.6 40.9 -76.2 -19.8 59.4 -13.4 -67.2 80 89 A E T 4 S- 0 0 93 -3,-0.2 -2,-0.3 1,-0.1 24,-0.3 0.962 102.5-166.1 -48.8 -58.5 57.7 -16.0 -69.5 81 90 A G < - 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