==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=5-JAN-2010 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER CELL ADHESION 18-JUL-07 2V6H . COMPND 2 MOLECULE: MYOSIN-BINDING PROTEIN C, CARDIAC-TYPE; . SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS; . AUTHOR L.GOVATA,L.CARPENTER,P.C.A.DA FONSECA,J.R.HELLIWELL, . 104 2 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 6730.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 62 59.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 5 4.8 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 39 37.5 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 1 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 1 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 2 1.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 7 6.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 6 5.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 2 1.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 1 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 1 1 0 0 0 1 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 151 A D 0 0 210 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0-153.0 -3.7 18.9 68.1 2 152 A D - 0 0 133 1,-0.1 3,-0.1 0, 0.0 0, 0.0 -0.228 360.0 -90.2 -75.8 151.7 -3.3 19.3 64.3 3 153 A P - 0 0 121 0, 0.0 2,-0.6 0, 0.0 -1,-0.1 -0.173 44.8-104.9 -54.0 149.0 -2.1 16.5 61.9 4 154 A I + 0 0 165 1,-0.1 4,-0.1 2,-0.1 2,-0.0 -0.718 45.9 178.7 -80.6 118.5 1.6 16.3 61.3 5 155 A G - 0 0 33 -2,-0.6 90,-0.1 2,-0.3 -1,-0.1 0.106 49.6 -71.4 -94.2-150.7 2.5 17.7 57.9 6 156 A L S S+ 0 0 21 1,-0.1 2,-1.0 82,-0.1 23,-0.5 0.669 102.6 94.4 -84.5 -15.8 5.9 18.0 56.2 7 157 A F + 0 0 24 1,-0.2 -2,-0.3 21,-0.1 3,-0.2 -0.694 37.7 172.1 -93.1 105.7 7.1 20.8 58.4 8 158 A V S S+ 0 0 93 19,-1.8 2,-0.4 -2,-1.0 20,-0.2 0.830 92.9 15.2 -63.5 -36.0 9.2 19.7 61.4 9 159 A M B S-A 27 0A 85 18,-1.7 18,-2.1 -3,-0.2 -1,-0.3 -0.961 83.5-148.7-141.1 122.9 9.7 23.4 61.7 10 160 A R - 0 0 113 -2,-0.4 15,-0.1 -3,-0.2 16,-0.1 -0.607 31.3 -96.5 -84.1 151.5 7.6 26.0 59.9 11 161 A P - 0 0 6 0, 0.0 2,-0.2 0, 0.0 -1,-0.1 -0.344 46.7-141.3 -59.8 145.9 8.9 29.4 58.8 12 162 A Q - 0 0 111 13,-0.1 2,-0.1 87,-0.1 87,-0.1 -0.704 19.1 -91.8-111.4 161.0 8.0 32.1 61.3 13 163 A D - 0 0 112 -2,-0.2 2,-0.3 86,-0.1 88,-0.2 -0.451 50.7-164.1 -61.5 143.8 6.9 35.7 61.2 14 164 A G E -d 101 0B 12 86,-2.8 88,-2.1 -2,-0.1 2,-0.4 -0.948 18.6-156.7-137.1 158.9 9.9 38.1 61.2 15 165 A E E +d 102 0B 137 -2,-0.3 2,-0.3 86,-0.2 88,-0.2 -0.985 17.1 169.6-133.0 140.5 10.8 41.7 61.8 16 166 A V E -d 103 0B 13 86,-2.2 88,-3.1 -2,-0.4 2,-0.3 -0.986 30.7-119.7-148.6 149.7 13.8 43.7 60.5 17 167 A T E > -d 104 0B 69 -2,-0.3 3,-2.7 86,-0.2 59,-0.3 -0.669 50.9 -82.8 -89.2 149.8 14.9 47.3 60.3 18 168 A V T 3 S+ 0 0 53 86,-2.6 59,-0.2 1,-0.3 -1,-0.1 -0.247 122.8 23.7 -50.1 135.9 15.6 48.9 56.9 19 169 A G T 3 S+ 0 0 40 57,-2.5 56,-0.5 1,-0.3 -1,-0.3 0.243 104.1 118.6 86.3 -11.5 19.2 47.9 55.8 20 170 A G < - 0 0 22 -3,-2.7 56,-2.5 56,-0.2 2,-0.3 -0.177 57.8-130.7 -81.1 173.6 19.0 44.8 58.1 21 171 A S + 0 0 81 53,-0.2 2,-0.3 54,-0.2 53,-0.2 -0.944 23.0 178.0-125.4 152.1 19.2 41.1 57.3 22 172 A I E - B 0 73A 28 51,-2.1 51,-3.3 -2,-0.3 2,-0.4 -0.989 16.4-144.0-153.7 145.6 17.0 38.3 58.3 23 173 A T E - B 0 72A 69 -2,-0.3 2,-0.3 49,-0.2 49,-0.2 -0.956 8.4-168.2-114.1 132.0 16.7 34.6 57.7 24 174 A F E + B 0 71A 7 47,-2.8 47,-2.7 -2,-0.4 2,-0.3 -0.866 18.9 178.4-106.3 148.7 13.6 32.5 57.3 25 175 A S E - B 0 70A 30 -2,-0.3 2,-0.3 45,-0.2 45,-0.2 -0.986 22.7-159.0-150.7 163.9 13.7 28.7 57.3 26 176 A A E - B 0 69A 0 43,-2.2 43,-2.7 -2,-0.3 2,-0.4 -0.992 10.4-153.1-136.9 146.3 11.9 25.4 57.2 27 177 A R E -AB 9 68A 101 -18,-2.1 -19,-1.8 -2,-0.3 -18,-1.7 -0.975 12.0-177.2-123.2 141.0 12.9 21.9 58.3 28 178 A V E - B 0 67A 1 39,-2.2 39,-3.4 -2,-0.4 -21,-0.1 -0.999 27.7-116.5-136.0 132.5 11.6 18.7 56.8 29 179 A A E B 0 66A 63 -23,-0.5 37,-0.2 -2,-0.4 36,-0.1 -0.378 360.0 360.0 -72.2 144.2 12.3 15.1 57.9 30 180 A G 0 0 75 35,-2.8 36,-0.1 34,-0.2 -1,-0.1 0.850 360.0 360.0 -86.9 360.0 14.1 12.9 55.4 31 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 32 185 A K 0 0 138 0, 0.0 59,-0.2 0, 0.0 3,-0.1 0.000 360.0 360.0 360.0 147.5 15.3 12.3 46.9 33 186 A P - 0 0 74 0, 0.0 -3,-0.1 0, 0.0 27,-0.0 -0.344 360.0-104.0 -56.0 144.2 15.4 15.4 48.9 34 187 A P - 0 0 9 0, 0.0 2,-0.4 0, 0.0 56,-0.2 -0.417 40.6-129.5 -62.6 154.0 12.2 17.3 49.5 35 188 A V E -E 89 0B 94 54,-2.5 54,-2.5 -3,-0.1 2,-0.3 -0.959 19.3-152.5-117.2 134.3 12.0 20.3 47.2 36 189 A V E -E 88 0B 20 -2,-0.4 2,-0.3 52,-0.2 52,-0.2 -0.706 12.3-173.5-106.3 151.1 11.2 23.9 48.3 37 190 A K E -E 87 0B 99 50,-2.2 50,-3.1 -2,-0.3 2,-0.4 -0.990 11.3-155.5-132.7 140.4 9.7 26.9 46.7 38 191 A W E -E 86 0B 3 -2,-0.3 8,-2.8 48,-0.2 9,-0.5 -0.955 15.7-175.1-115.2 139.4 9.5 30.3 48.4 39 192 A F E -EF 85 45B 19 46,-3.0 46,-2.4 -2,-0.4 2,-0.5 -0.957 26.1-148.9-129.5 151.0 6.9 32.9 47.3 40 193 A K E > S-EF 84 44B 57 4,-1.8 4,-1.7 -2,-0.3 44,-0.3 -0.982 83.9 -17.4-105.5 128.9 5.9 36.5 48.1 41 194 A G T 4 S- 0 0 37 42,-3.1 44,-0.1 -2,-0.5 -2,-0.0 -0.224 112.9 -52.0 68.9-165.5 2.1 36.7 47.6 42 195 A K T 4 S+ 0 0 142 1,-0.1 -1,-0.2 -3,-0.1 42,-0.0 0.885 132.4 20.5 -71.0 -36.3 0.3 33.9 45.7 43 196 A W T 4 S+ 0 0 208 -3,-0.2 2,-0.9 2,-0.0 -2,-0.2 0.542 83.0 106.4-122.4 -7.2 2.5 34.0 42.6 44 197 A V E < -F 40 0B 63 -4,-1.7 -4,-1.8 2,-0.0 2,-0.8 -0.693 54.0-151.6 -88.5 110.5 5.9 35.5 42.9 45 198 A D E > -F 39 0B 51 -2,-0.9 3,-2.0 -6,-0.2 -6,-0.2 -0.687 2.7-153.5 -75.8 107.8 8.4 32.8 42.9 46 199 A L G > S+ 0 0 8 -8,-2.8 3,-2.1 -2,-0.8 4,-0.2 0.649 86.2 76.7 -68.0 -11.2 11.4 34.1 44.9 47 200 A S G > S+ 0 0 67 -9,-0.5 3,-0.7 1,-0.3 -1,-0.3 0.814 92.5 56.3 -62.7 -25.2 13.9 31.9 43.1 48 201 A S G < S+ 0 0 87 -3,-2.0 -1,-0.3 1,-0.2 -2,-0.2 0.427 103.1 55.2 -82.3 0.2 13.5 34.4 40.3 49 202 A K G < S+ 0 0 126 -3,-2.1 4,-0.3 -4,-0.1 -1,-0.2 0.223 72.1 137.2-117.6 6.7 14.5 37.3 42.6 50 203 A V < + 0 0 69 -3,-0.7 2,-0.3 -4,-0.2 5,-0.2 -0.338 34.6 84.4 -62.6 133.6 17.9 36.0 43.8 51 204 A G S S- 0 0 34 3,-2.5 3,-0.3 -2,-0.1 -1,-0.1 -0.863 91.5 -13.4 170.1-136.3 20.5 38.7 43.9 52 205 A Q S S+ 0 0 153 -2,-0.3 3,-0.1 1,-0.2 -2,-0.1 0.727 136.7 33.1 -74.5 -20.5 21.8 41.4 46.2 53 206 A H S S+ 0 0 51 -4,-0.3 21,-2.7 1,-0.2 2,-0.4 0.504 120.1 42.2-108.6 -11.6 18.8 41.4 48.5 54 207 A L E +C 73 0A 17 -3,-0.3 -3,-2.5 19,-0.2 2,-0.3 -0.987 53.8 169.1-148.7 134.2 17.8 37.7 48.5 55 208 A Q E -C 72 0A 89 17,-2.3 17,-3.3 -2,-0.4 2,-0.4 -0.984 17.2-147.4-142.4 152.4 19.5 34.4 48.6 56 209 A L E +C 71 0A 56 -2,-0.3 2,-0.3 15,-0.2 15,-0.2 -0.934 13.7 179.5-119.6 141.7 18.4 30.7 49.0 57 210 A H E -C 70 0A 101 13,-2.3 13,-2.8 -2,-0.4 2,-0.4 -0.944 4.9-171.5-146.3 130.3 20.3 27.9 50.6 58 211 A D E +C 69 0A 84 -2,-0.3 2,-0.3 11,-0.2 11,-0.2 -0.927 16.1 160.7-118.4 138.5 19.3 24.2 51.1 59 212 A S E -C 68 0A 46 9,-2.6 9,-2.4 -2,-0.4 2,-0.4 -0.893 26.7-133.7-148.6 176.7 21.2 21.7 53.2 60 213 A Y E -C 67 0A 101 -2,-0.3 2,-0.7 7,-0.2 7,-0.2 -0.998 6.5-147.8-141.9 131.2 20.9 18.3 55.0 61 214 A D E >> -C 66 0A 83 5,-2.6 4,-1.7 -2,-0.4 5,-0.9 -0.889 10.8-161.6 -99.1 113.4 22.0 17.2 58.4 62 215 A R T 45S+ 0 0 198 -2,-0.7 -1,-0.1 1,-0.2 5,-0.0 0.895 84.3 54.5 -64.2 -39.4 23.0 13.5 58.3 63 216 A A T 45S+ 0 0 92 1,-0.2 -1,-0.2 2,-0.1 -2,-0.0 0.868 121.0 28.8 -62.0 -43.3 22.7 12.7 62.0 64 217 A S T 45S- 0 0 63 -3,-0.2 -1,-0.2 2,-0.1 -2,-0.2 0.590 99.0-131.7 -96.7 -13.7 19.2 13.9 62.4 65 218 A K T <5 + 0 0 131 -4,-1.7 -35,-2.8 1,-0.2 2,-0.4 0.770 58.0 144.2 64.3 28.6 18.1 13.2 58.8 66 219 A V E < -BC 29 61A 29 -5,-0.9 -5,-2.6 -37,-0.2 2,-0.4 -0.835 44.3-144.6-103.1 136.1 16.6 16.7 58.6 67 220 A Y E -BC 28 60A 41 -39,-3.4 -39,-2.2 -2,-0.4 2,-0.4 -0.839 14.5-155.3 -93.9 134.9 16.5 18.9 55.5 68 221 A L E -BC 27 59A 27 -9,-2.4 -9,-2.6 -2,-0.4 2,-0.5 -0.926 7.3-168.9-111.7 130.7 16.9 22.6 56.1 69 222 A F E -BC 26 58A 5 -43,-2.7 -43,-2.2 -2,-0.4 2,-0.4 -0.984 11.8-167.9-119.9 122.8 15.6 25.3 53.6 70 223 A E E -BC 25 57A 42 -13,-2.8 -13,-2.3 -2,-0.5 2,-0.5 -0.925 17.3-166.5-120.4 142.2 16.7 28.8 54.1 71 224 A L E -BC 24 56A 0 -47,-2.7 -47,-2.8 -2,-0.4 2,-0.6 -0.988 15.4-162.4-119.0 116.8 15.6 32.2 52.7 72 225 A H E -BC 23 55A 48 -17,-3.3 -17,-2.3 -2,-0.5 2,-0.5 -0.896 4.3-167.4-107.0 122.7 18.0 35.0 53.3 73 226 A I E -BC 22 54A 3 -51,-3.3 -51,-2.1 -2,-0.6 2,-0.3 -0.909 12.5-172.2-106.4 130.9 16.9 38.6 53.0 74 227 A T + 0 0 43 -21,-2.7 -53,-0.2 -2,-0.5 -54,-0.1 -0.929 59.0 8.2-129.7 152.7 19.6 41.3 53.0 75 228 A D S S- 0 0 67 -56,-0.5 -1,-0.2 -2,-0.3 -54,-0.2 0.954 90.1-138.5 46.4 60.8 19.7 45.1 53.1 76 229 A A - 0 0 0 -56,-2.5 -57,-2.5 -59,-0.3 -56,-0.2 -0.199 19.8-160.5 -57.8 135.8 16.0 45.1 53.7 77 230 A Q > - 0 0 68 -59,-0.2 3,-2.7 -3,-0.1 4,-0.4 -0.821 29.8-111.5-114.7 154.5 13.9 47.6 51.9 78 231 A P G > S+ 0 0 78 0, 0.0 3,-1.7 0, 0.0 25,-0.3 0.855 113.8 63.4 -53.8 -37.0 10.4 48.8 52.9 79 232 A A G 3 S+ 0 0 79 1,-0.3 -3,-0.0 3,-0.0 0, 0.0 0.695 87.3 73.2 -67.0 -11.7 8.8 47.2 49.9 80 233 A F G < S+ 0 0 45 -3,-2.7 -1,-0.3 2,-0.0 21,-0.2 0.689 71.8 109.5 -75.5 -16.9 9.9 43.8 51.2 81 234 A T < + 0 0 57 -3,-1.7 2,-0.3 -4,-0.4 21,-0.2 -0.291 55.1 69.6 -49.6 134.8 7.2 44.0 53.9 82 235 A G E S- G 0 101B 20 19,-2.7 19,-2.5 -41,-0.0 2,-0.5 -0.945 79.3 -30.0 152.6-164.1 4.4 41.5 53.2 83 236 A S E + G 0 100B 70 -2,-0.3 -42,-3.1 17,-0.2 2,-0.3 -0.775 50.3 179.6 -97.1 126.2 3.0 38.1 53.0 84 237 A Y E -EG 40 99B 10 15,-3.0 15,-2.2 -2,-0.5 2,-0.3 -0.764 10.6-156.4-122.6 164.3 5.2 35.1 52.1 85 238 A R E -EG 39 98B 33 -46,-2.4 -46,-3.0 -2,-0.3 2,-0.5 -1.000 7.3-151.9-148.8 141.2 4.8 31.4 51.7 86 239 A C E -EG 38 97B 0 11,-2.4 11,-1.9 -2,-0.3 2,-0.4 -0.976 15.9-169.0-111.2 124.2 7.0 28.3 51.8 87 240 A E E -EG 37 96B 32 -50,-3.1 -50,-2.2 -2,-0.5 2,-0.4 -0.939 3.1-173.5-115.0 134.5 6.0 25.3 49.7 88 241 A V E -EG 36 95B 0 7,-2.8 7,-2.3 -2,-0.4 2,-0.4 -0.965 1.8-168.3-127.8 145.0 7.6 21.9 50.0 89 242 A S E +EG 35 94B 49 -54,-2.5 -54,-2.5 -2,-0.4 2,-0.2 -0.974 6.3 179.2-126.8 144.6 7.1 18.8 47.9 90 243 A T - 0 0 46 3,-2.7 -84,-0.0 -2,-0.4 -2,-0.0 -0.732 52.8 -89.4-122.2 178.0 8.1 15.1 48.4 91 244 A K S S+ 0 0 150 -2,-0.2 3,-0.0 -59,-0.2 -2,-0.0 0.834 127.2 23.7 -54.6 -30.0 7.5 12.2 46.1 92 245 A D S S+ 0 0 142 1,-0.1 2,-0.3 0, 0.0 -1,-0.2 0.555 120.7 38.0-118.9 -13.1 4.2 11.6 48.0 93 246 A K - 0 0 124 2,-0.0 -3,-2.7 -87,-0.0 2,-0.3 -0.925 49.3-154.1-141.0 162.3 3.1 14.9 49.6 94 247 A F E + G 0 89B 118 -2,-0.3 2,-0.3 -5,-0.2 -5,-0.2 -0.975 21.4 165.3-129.1 147.9 2.8 18.6 49.3 95 248 A D E - G 0 88B 32 -7,-2.3 -7,-2.8 -2,-0.3 2,-0.3 -0.985 10.4-170.5-152.6 164.6 2.7 21.2 52.1 96 249 A C E - G 0 87B 39 -2,-0.3 2,-0.3 -9,-0.2 -9,-0.2 -0.978 6.9-155.7-154.9 171.6 3.0 24.9 52.6 97 250 A S E - G 0 86B 6 -11,-1.9 -11,-2.4 -2,-0.3 2,-0.3 -0.961 11.5-141.1-155.8 125.2 3.3 27.5 55.3 98 251 A N E + G 0 85B 113 -2,-0.3 2,-0.3 -13,-0.2 -13,-0.2 -0.626 27.5 158.8 -92.0 148.4 2.4 31.2 55.5 99 252 A F E - G 0 84B 1 -15,-2.2 -15,-3.0 -2,-0.3 2,-0.3 -0.969 25.8-127.9-158.7 168.9 4.5 33.8 57.2 100 253 A N E - G 0 83B 65 -2,-0.3 -86,-2.8 -17,-0.2 2,-0.4 -0.924 4.1-154.4-128.2 150.1 5.0 37.5 57.2 101 254 A L E -dG 14 82B 1 -19,-2.5 -19,-2.7 -2,-0.3 2,-0.5 -0.988 9.7-167.2-117.4 133.6 7.9 40.0 57.1 102 255 A T E -d 15 0B 55 -88,-2.1 -86,-2.2 -2,-0.4 2,-0.5 -0.990 5.7-158.1-118.4 126.1 7.7 43.5 58.5 103 256 A V E -d 16 0B 16 -2,-0.5 2,-0.4 -25,-0.3 -86,-0.2 -0.887 13.8-152.4-100.7 125.0 10.4 46.0 57.6 104 257 A H E d 17 0B 76 -88,-3.1 -86,-2.6 -2,-0.5 -2,-0.0 -0.831 360.0 360.0-104.4 141.3 10.7 48.9 60.1 105 258 A E 0 0 202 -2,-0.4 -1,-0.1 -88,-0.2 -88,-0.1 -0.536 360.0 360.0 87.8 360.0 11.9 52.4 59.4