==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=22-APR-2012 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER PROTEIN BINDING 18-DEC-11 3V64 . COMPND 2 MOLECULE: AGRIN; . SOURCE 2 ORGANISM_SCIENTIFIC: RATTUS NORVEGICUS; . AUTHOR Y.ZONG,B.ZHANG,S.GU,K.LEE,J.ZHOU,G.YAO,D.FIGUEIEDO,K.PERRY,L . 1053 6 12 10 2 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 46091.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 635 60.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 4 0.4 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 404 38.4 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 22 2.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 10 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 12 1.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 1 0.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 2 0.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 99 9.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 57 5.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 27 2.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 17 1.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 6 4 17 10 15 16 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 8 0 10 2 0 3 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 404 C Y 0 0 221 0, 0.0 2,-0.7 0, 0.0 3,-0.1 0.000 360.0 360.0 360.0 2.0 14.5 -7.8 23.4 2 405 C a - 0 0 24 1,-0.2 25,-0.2 2,-0.1 4,-0.1 -0.778 360.0-155.2 -91.1 120.8 16.4 -4.9 22.1 3 406 C S S S+ 0 0 4 23,-1.9 -1,-0.2 -2,-0.7 24,-0.1 0.859 84.5 0.6 -62.4 -38.2 14.0 -2.4 20.7 4 407 C Q S S- 0 0 23 22,-0.5 2,-0.3 10,-0.3 11,-0.1 0.739 120.3 -22.1-114.5 -76.6 16.6 -1.0 18.4 5 408 C G E -A 14 0A 10 9,-1.1 9,-3.7 2,-0.0 2,-0.5 -0.998 37.5-152.4-145.3 149.7 20.1 -2.3 18.3 6 409 C C E +A 13 0A 48 -2,-0.3 2,-0.4 7,-0.3 7,-0.2 -0.982 19.7 178.1-120.9 122.9 22.6 -4.2 20.4 7 410 C T + 0 0 97 5,-1.2 5,-0.3 -2,-0.5 -2,-0.0 -0.978 49.5 50.7-129.5 130.8 26.3 -3.7 19.9 8 411 C N 0 0 130 -2,-0.4 -1,-0.1 1,-0.4 5,-0.0 0.442 360.0 360.0 131.9 5.8 29.3 -5.3 21.8 9 412 C S 0 0 121 -3,-0.2 -1,-0.4 3,-0.1 -3,-0.0 -0.833 360.0 360.0-102.6 360.0 29.0 -9.1 21.9 10 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 11 416 C F 0 0 126 0, 0.0 2,-0.1 0, 0.0 -3,-0.1 0.000 360.0 360.0 360.0 34.7 25.3 -7.6 24.5 12 417 C Q - 0 0 155 -5,-0.3 -5,-1.2 1,-0.1 -3,-0.1 -0.446 360.0-117.3 -74.2 140.4 23.9 -4.3 25.8 13 418 C a E -A 6 0A 16 12,-0.6 2,-0.3 -7,-0.2 -7,-0.3 -0.373 26.0-146.1 -73.9 155.8 21.0 -2.6 23.8 14 419 C W E -A 5 0A 111 -9,-3.7 -9,-1.1 12,-0.1 2,-0.4 -0.910 1.6-141.6-125.0 150.9 21.5 0.9 22.3 15 420 C b - 0 0 37 -2,-0.3 3,-0.1 -11,-0.1 6,-0.1 -0.943 15.8-128.1-118.1 135.8 19.1 3.8 21.8 16 421 C E > - 0 0 71 -2,-0.4 3,-1.7 1,-0.2 187,-0.0 -0.247 45.7 -75.0 -72.2 162.3 18.9 6.1 18.8 17 422 C A T 3 S+ 0 0 76 1,-0.3 -1,-0.2 13,-0.1 3,-0.1 -0.344 123.9 29.0 -60.9 138.0 19.0 10.0 19.1 18 423 C G T 3 S+ 0 0 20 1,-0.3 13,-2.5 -3,-0.1 2,-0.3 0.460 112.0 91.8 83.6 3.7 15.6 11.3 20.3 19 424 C Y E < -B 30 0B 23 -3,-1.7 2,-0.4 11,-0.3 -1,-0.3 -0.913 58.0-160.4-127.8 155.7 15.2 8.0 22.1 20 425 C E E -B 29 0B 117 9,-3.1 9,-3.7 -2,-0.3 -4,-0.1 -1.000 27.1-109.6-139.7 133.9 16.1 6.8 25.6 21 426 C L E -B 28 0B 71 -2,-0.4 7,-0.2 7,-0.2 6,-0.1 -0.344 36.8-128.0 -62.0 139.7 16.5 3.3 27.0 22 427 C R > - 0 0 79 5,-2.9 3,-2.2 1,-0.1 -1,-0.1 -0.233 31.1 -91.9 -81.3 173.4 13.7 2.2 29.4 23 428 C P T 3 S+ 0 0 130 0, 0.0 -1,-0.1 0, 0.0 -2,-0.1 0.779 124.3 66.8 -54.6 -28.6 14.2 0.7 32.9 24 429 C D T 3 S- 0 0 84 1,-0.1 -3,-0.0 3,-0.0 -2,-0.0 0.514 101.2-135.3 -73.0 -6.1 14.1 -2.8 31.3 25 430 C R S < S+ 0 0 206 -3,-2.2 -12,-0.6 2,-0.2 -1,-0.1 0.401 93.5 68.4 69.4 -3.7 17.5 -1.9 29.6 26 431 C R S S+ 0 0 84 -24,-0.1 -23,-1.9 -14,-0.1 -22,-0.5 0.810 81.4 70.7-109.5 -53.8 16.0 -3.4 26.4 27 432 C S - 0 0 6 -25,-0.2 -5,-2.9 -24,-0.1 2,-0.5 -0.305 68.0-137.5 -74.7 152.0 13.2 -1.1 25.2 28 433 C b E -B 21 0B 1 -7,-0.2 2,-0.4 -2,-0.0 -7,-0.2 -0.954 14.0-161.3-112.8 126.0 13.6 2.4 23.7 29 434 C K E -B 20 0B 78 -9,-3.7 -9,-3.1 -2,-0.5 2,-0.3 -0.896 22.2-118.4-108.1 135.7 11.3 5.3 24.7 30 435 C A E -B 19 0B 8 -2,-0.4 2,-0.3 -11,-0.2 -11,-0.3 -0.560 19.9-130.2 -79.3 128.0 11.0 8.4 22.6 31 436 C L S S+ 0 0 74 -13,-2.5 257,-0.2 -2,-0.3 -1,-0.1 -0.601 73.3 49.6 -73.1 131.7 12.0 11.8 24.1 32 437 C G S S- 0 0 58 -2,-0.3 254,-0.0 255,-0.1 -14,-0.0 -0.930 98.8 -19.9 137.5-161.0 9.3 14.4 23.6 33 438 C P S S- 0 0 81 0, 0.0 3,-0.1 0, 0.0 -3,-0.0 -0.159 82.2 -78.3 -69.4 172.8 5.5 14.5 24.2 34 439 C E - 0 0 104 1,-0.1 -3,-0.0 252,-0.0 0, 0.0 -0.551 61.0 -94.4 -72.1 140.5 3.3 11.4 24.6 35 440 C P - 0 0 16 0, 0.0 2,-0.4 0, 0.0 251,-0.2 -0.188 39.1-159.1 -58.2 144.8 2.5 9.7 21.2 36 441 C V E -C 285 0C 25 249,-1.3 249,-4.9 13,-0.1 2,-0.6 -0.989 11.4-136.5-127.7 137.2 -0.7 10.6 19.4 37 442 C L E -CD 284 48C 0 11,-3.1 11,-1.4 -2,-0.4 2,-0.5 -0.844 17.3-165.1 -95.5 118.8 -2.6 8.6 16.8 38 443 C L E +CD 283 47C 0 245,-1.5 245,-1.4 -2,-0.6 2,-0.3 -0.905 19.7 163.1-104.0 124.1 -3.9 10.7 13.8 39 444 C F E -CD 282 46C 0 7,-2.2 7,-2.6 -2,-0.5 2,-0.4 -0.975 35.5-120.5-144.3 156.2 -6.4 8.9 11.6 40 445 C A E - D 0 45C 0 241,-2.3 240,-3.0 -2,-0.3 5,-0.2 -0.798 16.1-176.1 -99.1 136.7 -9.1 9.6 9.0 41 446 C N - 0 0 2 3,-3.4 2,-3.0 -2,-0.4 237,-0.2 -0.008 44.8-116.2-119.9 26.4 -12.7 8.6 9.5 42 447 C R B S+F 65 0D 41 23,-1.6 23,-2.8 2,-0.3 24,-0.1 -0.297 115.8 37.9 68.1 -56.6 -14.2 9.5 6.1 43 448 C I S S+ 0 0 64 -2,-3.0 19,-2.1 21,-0.2 18,-0.3 0.642 128.6 23.0 -93.3 -19.2 -16.4 12.1 7.8 44 449 C D - 0 0 4 21,-0.2 -3,-3.4 17,-0.2 2,-0.4 -0.865 61.3-133.9-138.9 167.4 -13.8 13.2 10.3 45 450 C I E -DE 40 59C 0 14,-1.8 13,-2.0 -2,-0.3 14,-1.5 -0.994 41.2-173.7-122.1 123.2 -10.1 13.4 11.2 46 451 C R E -DE 39 57C 50 -7,-2.6 -7,-2.2 -2,-0.4 2,-0.4 -0.764 20.4-145.5-124.9 162.6 -9.5 12.3 14.7 47 452 C Q E -DE 38 56C 35 9,-3.4 9,-2.9 -2,-0.3 2,-0.3 -0.958 15.4-179.9-127.4 144.4 -6.9 12.0 17.4 48 453 C V E -DE 37 55C 2 -11,-1.4 -11,-3.1 -2,-0.4 7,-0.2 -0.997 26.3-138.7-142.1 143.5 -6.3 9.3 20.2 49 454 C L > - 0 0 37 5,-1.5 3,-1.2 -2,-0.3 5,-0.3 -0.930 39.5-122.5 -97.3 110.5 -3.8 8.7 23.0 50 455 C P T 3 S+ 0 0 14 0, 0.0 3,-0.1 0, 0.0 4,-0.1 -0.201 90.8 18.3 -54.7 140.2 -3.2 5.0 22.9 51 456 C H T 3 S+ 0 0 109 1,-0.2 2,-0.3 2,-0.0 221,-0.0 0.677 116.3 76.6 68.8 21.9 -3.9 3.0 26.1 52 457 C R S < S- 0 0 126 -3,-1.2 -1,-0.2 2,-0.1 -3,-0.1 -0.930 87.2-113.6-147.0 170.4 -6.1 5.8 27.5 53 458 C S + 0 0 95 -2,-0.3 2,-0.3 -3,-0.1 -2,-0.0 0.394 67.1 129.9 -90.8 3.9 -9.6 7.0 26.8 54 459 C E + 0 0 103 -5,-0.3 -5,-1.5 -4,-0.1 2,-0.4 -0.440 29.6 172.7 -68.5 125.0 -8.7 10.4 25.3 55 460 C Y E -E 48 0C 64 -2,-0.3 2,-0.4 -7,-0.2 -7,-0.2 -0.973 6.2-177.2-142.0 114.5 -10.5 10.9 22.0 56 461 C T E -E 47 0C 58 -9,-2.9 -9,-3.4 -2,-0.4 2,-0.8 -0.959 22.4-142.6-126.0 131.4 -10.4 14.2 20.0 57 462 C L E +E 46 0C 70 -2,-0.4 -11,-0.2 -11,-0.2 3,-0.1 -0.813 22.6 178.2 -90.3 111.0 -12.2 15.2 16.8 58 463 C L E + 0 0 30 -13,-2.0 2,-0.4 -2,-0.8 -12,-0.2 0.977 65.3 17.7 -80.4 -60.7 -9.7 17.4 14.9 59 464 C L E S-E 45 0C 10 -14,-1.5 -14,-1.8 36,-0.0 -1,-0.3 -0.962 73.7-167.1-119.1 133.2 -11.4 18.3 11.7 60 465 C N + 0 0 83 -2,-0.4 -16,-0.1 -16,-0.2 -14,-0.0 -0.731 56.4 35.6-118.3 164.4 -15.2 18.0 11.1 61 466 C N S S+ 0 0 143 -18,-0.3 2,-0.2 -2,-0.2 -1,-0.2 0.840 70.9 158.1 64.3 38.4 -17.7 18.0 8.3 62 467 C L - 0 0 24 -19,-2.1 -1,-0.2 -3,-0.2 3,-0.1 -0.624 38.4-142.8 -86.4 151.1 -15.5 16.3 5.7 63 468 C E S S- 0 0 101 -2,-0.2 2,-0.3 1,-0.2 -1,-0.1 0.983 82.1 -14.4 -76.5 -69.3 -17.0 14.5 2.7 64 469 C N - 0 0 2 -21,-0.1 18,-1.6 497,-0.0 2,-0.7 -0.770 69.4-171.6-139.9 91.6 -14.8 11.5 2.3 65 470 C A E +FG 42 81D 0 -23,-2.8 -23,-1.6 -2,-0.3 16,-0.2 -0.762 23.3 145.3 -90.4 116.6 -11.5 11.6 4.2 66 471 C I E + 0 0 0 14,-2.1 215,-1.4 -2,-0.7 -26,-0.3 0.756 52.9 35.0-113.1 -79.8 -9.2 8.8 3.3 67 472 C A E + 0 0 0 41,-0.5 13,-2.4 213,-0.1 -1,-0.3 -0.630 63.5 174.7 -88.4 140.6 -5.4 9.4 3.2 68 473 C L E + G 0 79D 0 -2,-0.3 2,-0.3 11,-0.2 11,-0.2 -0.999 10.1 167.7-144.1 146.8 -3.7 11.7 5.6 69 474 C D E - G 0 78D 13 9,-2.3 9,-3.6 -2,-0.3 2,-0.3 -0.923 19.2-138.8-144.7 172.7 -0.2 12.8 6.5 70 475 C F E - G 0 77D 2 -2,-0.3 2,-0.5 7,-0.3 7,-0.3 -0.922 12.5-144.0-136.8 156.2 1.4 15.6 8.6 71 476 C H E > - G 0 76D 1 5,-3.5 5,-2.7 -2,-0.3 4,-0.5 -0.948 20.6-167.6-119.5 106.5 4.2 18.2 8.7 72 477 C H T > 5S+ 0 0 70 -2,-0.5 3,-0.8 1,-0.2 -1,-0.2 0.913 80.7 41.4 -64.7 -51.6 5.3 18.5 12.3 73 478 C R T 3 5S+ 0 0 179 1,-0.3 -1,-0.2 217,-0.1 -2,-0.0 0.702 119.2 46.2 -75.5 -19.9 7.5 21.6 12.4 74 479 C R T 3 5S- 0 0 120 2,-0.1 -1,-0.3 -3,-0.1 -2,-0.2 0.443 107.0-128.6 -94.7 -3.2 5.1 23.5 10.1 75 480 C E T < 5 + 0 0 105 -3,-0.8 17,-2.6 -4,-0.5 2,-0.3 0.900 59.0 146.3 54.0 45.7 2.2 22.3 12.2 76 481 C L E < -GH 71 91D 22 -5,-2.7 -5,-3.5 15,-0.3 2,-0.4 -0.868 35.8-164.9-115.2 146.0 0.4 21.2 9.0 77 482 C V E -GH 70 90D 0 13,-2.8 13,-1.9 -2,-0.3 2,-0.4 -0.998 8.5-162.8-129.1 127.9 -1.9 18.2 8.3 78 483 C F E +GH 69 89D 0 -9,-3.6 -9,-2.3 -2,-0.4 2,-0.3 -0.869 13.3 167.6-109.6 143.7 -2.7 17.0 4.8 79 484 C W E -GH 68 88D 5 9,-2.5 9,-2.4 -2,-0.4 2,-0.3 -0.984 24.3-133.1-148.3 159.5 -5.6 14.8 3.7 80 485 C S E - H 0 87D 0 -13,-2.4 -14,-2.1 -2,-0.3 2,-0.5 -0.852 9.0-151.4-112.1 149.4 -7.4 13.6 0.6 81 486 C D E >> -GH 65 86D 2 5,-2.9 5,-1.8 -2,-0.3 4,-1.3 -0.977 5.1-168.7-123.1 119.0 -11.1 13.5 -0.2 82 487 C V T 45S+ 0 0 2 -18,-1.6 -1,-0.1 -2,-0.5 -17,-0.1 0.873 85.6 48.1 -79.7 -39.4 -12.3 10.8 -2.6 83 488 C T T 45S+ 0 0 23 1,-0.2 481,-0.3 -19,-0.2 -1,-0.2 0.821 120.8 40.5 -68.5 -28.9 -15.9 11.9 -3.3 84 489 C L T 45S- 0 0 76 -20,-0.2 -2,-0.2 2,-0.1 -1,-0.2 0.718 100.3-134.2 -88.1 -24.6 -14.6 15.5 -3.9 85 490 C D T <5 + 0 0 43 -4,-1.3 2,-0.3 1,-0.3 20,-0.3 0.920 63.0 126.6 64.0 47.6 -11.5 14.5 -5.8 86 491 C R E < -H 81 0D 82 -5,-1.8 -5,-2.9 22,-0.1 2,-0.4 -0.893 55.0-148.5-131.6 157.9 -9.5 17.0 -3.7 87 492 C I E -HI 80 101D 0 14,-2.3 13,-2.7 -2,-0.3 14,-1.9 -0.977 28.4-175.5-126.6 117.5 -6.3 17.1 -1.6 88 493 C L E -HI 79 99D 25 -9,-2.4 -9,-2.5 -2,-0.4 2,-0.3 -0.708 11.5-163.2-112.7 161.8 -6.6 19.6 1.3 89 494 C R E +HI 78 98D 45 9,-2.2 9,-2.8 -2,-0.2 2,-0.3 -0.967 11.5 169.0-139.5 155.1 -4.3 20.9 4.0 90 495 C A E -H 77 0D 1 -13,-1.9 -13,-2.8 -2,-0.3 6,-0.2 -0.979 44.4 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7,-0.5 7,-3.2 -125,-0.2 2,-0.5 -0.731 12.5-179.8 -90.5 122.8 -37.3 -2.4 -13.1 698 1924 B V E -QN 703 571N 20 -127,-3.1 -127,-2.9 -2,-0.5 2,-0.7 -0.957 8.8-175.2-127.0 112.9 -33.8 -1.9 -14.5 699 1925 B V E > S-QN 702 570N 32 3,-1.8 3,-1.5 -2,-0.5 2,-0.5 -0.927 73.7 -39.3-107.1 109.9 -31.5 0.7 -13.0 700 1926 B G T 3 S- 0 0 24 -131,-3.5 -129,-0.1 -2,-0.7 -132,-0.0 -0.653 122.1 -34.0 70.6-123.3 -28.4 0.9 -15.1 701 1927 B H T 3 S+ 0 0 53 -2,-0.5 2,-0.4 2,-0.1 -1,-0.2 0.367 117.6 101.6-107.1 0.9 -27.6 -2.7 -16.1 702 1928 B R E < S-Q 699 0N 109 -3,-1.5 -3,-1.8 1,-0.0 2,-0.6 -0.722 71.8-130.0 -91.0 133.6 -28.9 -4.2 -12.8 703 1929 B Q E -Q 698 0N 93 -2,-0.4 2,-0.3 -5,-0.2 -5,-0.2 -0.723 30.5-133.3 -78.2 120.6 -32.3 -5.8 -12.5 704 1930 B L - 0 0 10 -7,-3.2 2,-0.8 -2,-0.6 -7,-0.5 -0.596 1.3-138.0 -83.6 133.0 -34.0 -4.3 -9.5 705 1931 B H > - 0 0 96 -2,-0.3 4,-2.1 1,-0.2 5,-0.3 -0.823 15.6-154.8 -88.0 108.8 -35.8 -6.4 -6.9 706 1932 B L T 4 S+ 0 0 5 -2,-0.8 4,-0.4 1,-0.2 -1,-0.2 0.844 85.7 28.2 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