==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=5-JAN-2010 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 09-JUN-08 2VVJ . COMPND 2 MOLECULE: GREEN TO RED PHOTOCONVERTIBLE GPF-LIKE PROTEIN . SOURCE 2 ORGANISM_SCIENTIFIC: LOBOPHYLLIA HEMPRICHII; . AUTHOR V.ADAM,M.LELIMOUSIN,S.BOEHME,G.DESFONDS,K.NIENHAUS, . 877 8 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 34093.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 649 74.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 52 5.9 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 411 46.9 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 10 1.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 12 1.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 74 8.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 77 8.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 17 1.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 8 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 4 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 0 4 0 5 3 4 4 8 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 3 A A 0 0 64 0, 0.0 2,-0.6 0, 0.0 76,-0.1 0.000 360.0 360.0 360.0 -33.0 11.6 -28.6 40.9 2 4 A I - 0 0 30 77,-0.1 106,-0.1 74,-0.1 74,-0.0 -0.799 360.0-166.8 -90.8 118.9 8.9 -26.5 42.7 3 5 A K - 0 0 72 -2,-0.6 29,-0.2 1,-0.1 3,-0.1 -0.717 31.8-113.8-101.1 154.3 9.5 -26.2 46.5 4 6 A P S S+ 0 0 86 0, 0.0 2,-0.5 0, 0.0 28,-0.2 0.816 106.2 35.0 -55.6 -35.9 6.9 -25.0 49.0 5 7 A D S S+ 0 0 96 25,-0.2 2,-0.3 26,-0.1 25,-0.2 -0.960 78.4 172.9-128.0 114.1 8.9 -21.8 49.8 6 8 A M E -A 29 0A 3 23,-2.4 23,-2.4 -2,-0.5 103,-0.2 -0.858 24.8-129.5-127.2 155.7 10.9 -20.1 47.1 7 9 A K E -Ab 28 109A 111 101,-3.4 103,-2.7 -2,-0.3 2,-0.4 -0.595 16.6-149.3 -97.8 159.6 12.9 -16.9 46.5 8 10 A I E -Ab 27 110A 3 19,-2.5 19,-2.8 101,-0.2 2,-0.4 -0.986 10.6-174.8-136.3 122.5 12.5 -14.5 43.6 9 11 A N E +Ab 26 111A 60 101,-3.3 103,-1.6 -2,-0.4 2,-0.3 -0.930 16.4 175.4-113.0 143.4 15.2 -12.3 42.0 10 12 A L E -Ab 25 112A 2 15,-2.1 15,-2.6 -2,-0.4 2,-0.4 -1.000 24.7-173.3-152.1 143.0 14.3 -9.8 39.3 11 13 A R E -Ab 24 113A 95 101,-2.1 103,-2.6 -2,-0.3 2,-0.4 -0.966 13.2-171.7-135.1 123.4 15.6 -7.0 37.1 12 14 A M E -Ab 23 114A 0 11,-3.1 11,-2.9 -2,-0.4 2,-0.4 -0.945 2.0-174.9-111.9 134.0 13.3 -4.9 35.0 13 15 A E E +Ab 22 115A 49 101,-2.2 103,-1.7 -2,-0.4 2,-0.3 -0.986 23.0 141.6-121.8 140.8 14.5 -2.5 32.4 14 16 A G E -Ab 21 116A 2 7,-2.5 7,-2.4 -2,-0.4 2,-0.3 -0.953 40.6-133.7-163.9-177.7 12.2 -0.2 30.5 15 17 A N E -Ab 20 117A 31 101,-2.1 103,-2.4 -2,-0.3 2,-0.4 -0.917 15.5-177.4-154.0 118.6 11.0 3.0 28.9 16 18 A V E > S-Ab 19 118A 1 3,-1.9 3,-2.3 -2,-0.3 103,-0.2 -0.990 78.8 -15.4-122.2 126.3 7.6 4.7 29.2 17 19 A N T 3 S- 0 0 33 101,-2.4 102,-0.1 -2,-0.4 -1,-0.1 0.800 131.4 -51.1 50.4 34.8 6.9 7.9 27.2 18 20 A G T 3 S+ 0 0 47 100,-0.4 2,-0.7 1,-0.2 -1,-0.3 0.266 109.6 125.5 92.0 -10.2 10.7 8.2 26.6 19 21 A H E < -A 16 0A 59 -3,-2.3 -3,-1.9 26,-0.0 -1,-0.2 -0.752 50.1-148.1 -91.3 112.0 11.7 7.8 30.2 20 22 A H E +A 15 0A 85 -2,-0.7 2,-0.3 -5,-0.2 -5,-0.2 -0.471 23.1 171.7 -76.5 144.5 14.3 5.1 30.8 21 23 A F E -A 14 0A 0 -7,-2.4 -7,-2.5 -2,-0.2 2,-0.4 -0.993 24.9-156.0-151.3 155.8 14.3 3.1 34.1 22 24 A V E -AC 13 44A 3 22,-2.0 21,-2.5 -2,-0.3 22,-1.6 -0.998 15.5-174.4-130.1 134.7 15.8 0.1 35.8 23 25 A I E -AC 12 42A 0 -11,-2.9 -11,-3.1 -2,-0.4 2,-0.4 -0.997 8.0-157.4-133.9 128.7 14.1 -1.6 38.8 24 26 A D E -AC 11 41A 52 17,-2.7 17,-2.7 -2,-0.4 2,-0.4 -0.833 11.1-165.6-103.7 145.3 15.5 -4.4 40.9 25 27 A G E -AC 10 40A 1 -15,-2.6 -15,-2.1 -2,-0.4 2,-0.4 -0.982 15.1-179.4-133.5 143.1 13.4 -6.9 42.9 26 28 A D E +AC 9 39A 101 13,-1.9 13,-2.8 -2,-0.4 2,-0.2 -0.972 28.3 140.9-137.3 122.7 13.9 -9.4 45.7 27 29 A G E -AC 8 38A 13 -19,-2.8 -19,-2.5 -2,-0.4 2,-0.3 -0.832 36.6-130.7-147.8-176.8 11.0 -11.5 47.0 28 30 A T E +AC 7 37A 83 9,-1.9 9,-2.5 -21,-0.3 2,-0.3 -0.966 23.1 175.6-139.8 156.9 9.6 -14.7 48.3 29 31 A G E -AC 6 36A 7 -23,-2.4 -23,-2.4 -2,-0.3 7,-0.3 -0.980 33.4-127.7-156.4 168.1 6.5 -16.8 47.5 30 32 A K E >>> - C 0 35A 78 5,-3.1 3,-1.5 -2,-0.3 5,-1.5 -0.888 21.7-167.2-121.1 94.0 4.5 -20.0 48.1 31 33 A P T 345S+ 0 0 0 0, 0.0 34,-0.8 0, 0.0 3,-0.5 0.847 86.2 50.1 -51.3 -42.5 3.9 -21.5 44.6 32 34 A F T 345S+ 0 0 107 1,-0.2 32,-0.1 -28,-0.2 -27,-0.1 0.611 114.9 44.2 -77.4 -12.0 1.2 -24.0 45.8 33 35 A E T <45S- 0 0 94 -3,-1.5 33,-0.4 -29,-0.2 -1,-0.2 0.480 107.2-127.3-104.2 -8.7 -0.7 -21.3 47.7 34 36 A G T <5 + 0 0 0 -4,-1.1 176,-2.4 -3,-0.5 2,-0.4 0.843 64.7 128.7 66.2 37.0 -0.4 -18.8 44.8 35 37 A K E < +CD 30 209A 74 -5,-1.5 -5,-3.1 174,-0.2 2,-0.3 -0.971 25.3 160.0-127.7 139.2 1.0 -16.0 46.9 36 38 A Q E -CD 29 208A 11 172,-2.1 172,-2.7 -2,-0.4 2,-0.3 -0.986 13.7-170.2-151.0 153.3 4.1 -13.9 46.4 37 39 A S E -CD 28 207A 39 -9,-2.5 -9,-1.9 -2,-0.3 2,-0.3 -0.977 8.5-170.0-142.5 155.1 5.5 -10.6 47.6 38 40 A M E -CD 27 206A 6 168,-2.3 168,-2.9 -2,-0.3 2,-0.6 -1.000 22.4-134.2-148.7 140.2 8.6 -8.6 46.5 39 41 A D E -CD 26 205A 86 -13,-2.8 -13,-1.9 -2,-0.3 2,-0.3 -0.885 31.8-161.3 -94.1 123.9 10.6 -5.6 47.6 40 42 A L E -CD 25 204A 6 164,-3.8 164,-2.0 -2,-0.6 2,-0.4 -0.838 11.1-171.7-111.1 143.3 11.3 -3.3 44.6 41 43 A E E -CD 24 203A 75 -17,-2.7 -17,-2.7 -2,-0.3 2,-0.6 -0.972 21.8-136.0-138.4 122.4 13.9 -0.6 44.2 42 44 A V E +C 23 0A 16 160,-2.6 -19,-0.3 -2,-0.4 3,-0.1 -0.657 25.7 175.4 -73.5 117.3 14.2 1.9 41.3 43 45 A K E + 0 0 106 -21,-2.5 2,-0.3 -2,-0.6 -20,-0.2 0.761 66.7 21.6 -95.7 -33.3 17.9 1.9 40.5 44 46 A E E S+C 22 0A 101 -22,-1.6 -22,-2.0 1,-0.1 -1,-0.3 -0.994 117.5 27.5-135.9 141.6 17.8 4.2 37.5 45 47 A G S S- 0 0 28 -2,-0.3 -1,-0.1 1,-0.3 -2,-0.0 0.517 83.4-172.6 90.5 6.8 15.3 6.8 36.4 46 48 A G + 0 0 40 1,-0.1 -1,-0.3 2,-0.1 2,-0.1 -0.572 61.4 44.8 -80.4 144.9 13.9 7.7 39.8 47 49 A P S S- 0 0 70 0, 0.0 -1,-0.1 0, 0.0 154,-0.1 0.491 90.4-139.0 -78.9 138.1 11.6 9.3 40.6 48 50 A L - 0 0 4 152,-0.5 4,-0.1 1,-0.1 -2,-0.1 -0.376 1.8-147.9 -62.1 134.5 9.4 7.8 37.9 49 51 A P S S+ 0 0 45 0, 0.0 2,-0.3 0, 0.0 -1,-0.1 0.463 76.2 62.4 -84.9 0.2 7.2 10.3 36.2 50 52 A F S S- 0 0 3 79,-0.1 78,-0.2 80,-0.1 2,-0.1 -0.810 101.3 -75.7-122.5 162.8 4.4 7.8 35.6 51 53 A A > - 0 0 4 76,-1.9 3,-1.1 -2,-0.3 4,-0.3 -0.350 32.8-144.9 -58.4 125.5 2.1 5.8 37.8 52 54 A F G >> S+ 0 0 8 1,-0.2 3,-1.9 2,-0.2 4,-0.7 0.797 91.1 77.3 -62.5 -28.6 3.9 2.8 39.3 53 55 A D G >4 S+ 0 0 7 1,-0.3 3,-0.8 2,-0.2 4,-0.5 0.793 81.1 65.4 -53.4 -34.6 0.7 0.7 39.1 54 56 A I G <4 S+ 0 0 0 -3,-1.1 -1,-0.3 1,-0.2 -2,-0.2 0.762 105.1 46.4 -61.8 -23.1 1.1 0.2 35.3 55 57 A L G X4 S+ 0 0 0 -3,-1.9 3,-2.0 -4,-0.3 4,-0.3 0.703 87.4 89.4 -87.3 -23.0 4.3 -1.8 36.0 56 58 A T G X< S+ 0 0 3 -3,-0.8 3,-1.1 -4,-0.7 -1,-0.1 0.760 83.4 50.3 -62.0 -37.6 3.1 -4.1 38.8 57 59 A T G 3 S+ 0 0 38 -4,-0.5 -1,-0.3 1,-0.2 -2,-0.1 0.621 103.6 63.7 -73.0 -11.9 1.7 -7.1 36.8 58 60 A A G < 0 0 1 -3,-2.0 -1,-0.2 -4,-0.1 -2,-0.2 0.535 360.0 360.0 -92.2 6.1 5.0 -7.3 34.9 59 61 A X < 0 0 11 -3,-1.1 -2,-0.1 -4,-0.3 -48,-0.1 0.797 360.0 360.0 -88.7 360.0 7.3 -8.1 38.0 60 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 61 65 A N > 0 0 5 0, 0.0 3,-2.0 0, 0.0 -3,-0.0 0.000 360.0 360.0 360.0 104.9 4.9 -15.2 37.3 62 66 A R T 3 + 0 0 12 1,-0.3 13,-0.2 13,-0.0 14,-0.2 0.458 360.0 85.4 -74.3 1.3 2.0 -17.6 37.0 63 67 A V T 3 S+ 0 0 0 1,-0.2 2,-1.4 12,-0.2 -1,-0.3 0.794 80.3 70.6 -64.5 -26.9 3.9 -20.3 38.8 64 68 A F S < S+ 0 0 0 -3,-2.0 2,-0.3 -30,-0.1 -1,-0.2 -0.617 78.0 105.2 -90.9 74.1 2.5 -18.5 41.9 65 69 A A S S- 0 0 0 -2,-1.4 2,-0.9 -34,-0.8 -31,-0.4 -0.962 74.5-119.1-151.3 134.2 -1.0 -19.6 41.4 66 70 A E B -e 211 0A 93 144,-2.4 146,-2.2 -33,-0.4 -34,-0.1 -0.689 35.4-168.3 -78.8 106.9 -3.0 -22.2 43.3 67 71 A Y - 0 0 28 -2,-0.9 2,-0.1 144,-0.2 146,-0.1 -0.850 12.0-138.5 -98.0 129.0 -4.0 -24.9 40.7 68 72 A P > - 0 0 40 0, 0.0 3,-1.5 0, 0.0 145,-0.0 -0.395 29.3-104.2 -77.6 165.3 -6.5 -27.6 41.6 69 73 A D T 3 S+ 0 0 172 1,-0.3 4,-0.1 -2,-0.1 -2,-0.0 0.768 115.0 61.3 -60.1 -31.6 -6.0 -31.2 40.5 70 74 A H T 3 S+ 0 0 130 2,-0.1 2,-0.4 1,-0.0 -1,-0.3 0.443 96.9 67.3 -81.9 0.9 -8.5 -31.1 37.7 71 75 A I S < S- 0 0 16 -3,-1.5 2,-0.1 141,-0.1 141,-0.1 -0.986 88.1-116.5-124.4 127.5 -6.6 -28.3 35.7 72 76 A Q - 0 0 52 -2,-0.4 2,-1.0 1,-0.1 3,-0.1 -0.399 21.4-141.3 -61.2 129.5 -3.2 -28.8 34.1 73 77 A D > + 0 0 20 1,-0.2 4,-1.5 -2,-0.1 5,-0.1 -0.777 23.3 177.4 -97.2 90.7 -0.6 -26.6 35.7 74 78 A Y T 4 S+ 0 0 17 -2,-1.0 4,-0.3 1,-0.2 -1,-0.2 0.793 75.6 53.0 -61.4 -32.1 1.5 -25.4 32.7 75 79 A F T >4 S+ 0 0 0 2,-0.2 3,-0.9 -13,-0.2 -1,-0.2 0.915 108.1 44.9 -81.3 -44.4 3.7 -23.2 34.8 76 80 A K G >4 S+ 0 0 21 1,-0.2 3,-0.9 -14,-0.2 -1,-0.2 0.821 112.9 54.9 -66.1 -29.8 4.9 -25.6 37.5 77 81 A Q G 3< S+ 0 0 113 -4,-1.5 -1,-0.2 1,-0.2 -2,-0.2 0.568 94.4 70.7 -76.2 -11.0 5.4 -28.2 34.7 78 82 A S G <> S+ 0 0 1 -3,-0.9 4,-3.2 -4,-0.3 98,-0.3 0.509 88.0 127.8 -83.4 -6.8 7.7 -25.6 32.9 79 83 A F T <4 + 0 0 17 -3,-0.9 -77,-0.1 2,-0.2 25,-0.0 -0.767 48.3 25.5-108.6 154.9 10.4 -26.0 35.5 80 84 A P T 4 S+ 0 0 83 0, 0.0 25,-0.6 0, 0.0 -1,-0.2 -0.961 131.6 41.6 -73.4 -18.6 13.4 -26.5 36.1 81 85 A K T 4 S- 0 0 121 1,-0.2 -2,-0.2 23,-0.1 24,-0.2 0.886 102.4-148.9 -66.2 -41.1 14.1 -25.2 32.6 82 86 A G < - 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