==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=10-MAR-2010 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER HYDROLASE 15-DEC-08 2W5W . COMPND 2 MOLECULE: ALKALINE PHOSPHATASE; . SOURCE 2 ORGANISM_SCIENTIFIC: ANTARCTIC BACTERIUM TAB5; . AUTHOR D.KOUTSIOULIS,A.LYSKOWSKI,S.MAKI,E.GUTHRIE,G.FELLER, . 692 2 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 23968.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 483 69.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 84 12.1 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 56 8.1 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 4 0.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 2 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 2 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 35 5.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 90 13.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 163 23.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 10 1.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 5 0 4 4 0 1 5 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 8 0 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 8 2 2 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 2 2 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 30 A Q 0 0 237 0, 0.0 2,-0.1 0, 0.0 320,-0.1 0.000 360.0 360.0 360.0 105.6 -4.0 39.2 -17.3 2 31 A L - 0 0 60 1,-0.2 2,-0.1 319,-0.1 0, 0.0 -0.411 360.0 -93.3 -88.0 171.9 -4.9 38.4 -13.7 3 32 A K - 0 0 135 -2,-0.1 -1,-0.2 316,-0.1 0, 0.0 -0.256 32.6-116.7 -82.8 174.9 -2.9 39.5 -10.6 4 33 A T - 0 0 43 338,-0.1 2,-0.2 -2,-0.1 338,-0.2 -0.944 21.9-145.2-120.5 110.4 -0.2 37.5 -8.9 5 34 A P - 0 0 2 0, 0.0 260,-0.2 0, 0.0 259,-0.1 -0.523 5.2-157.5 -74.5 142.0 -0.7 36.4 -5.3 6 35 A K S S+ 0 0 71 258,-1.7 2,-0.3 -2,-0.2 259,-0.2 0.846 79.9 33.5 -77.0 -39.6 2.3 36.2 -3.0 7 36 A N E -a 265 0A 5 257,-2.0 259,-2.7 212,-0.2 2,-0.4 -0.842 67.4-157.7-120.6 152.2 0.7 33.8 -0.6 8 37 A V E -ab 266 221A 0 212,-2.3 214,-2.1 -2,-0.3 2,-0.5 -0.998 5.4-170.9-132.9 134.3 -1.7 30.9 -1.0 9 38 A I E -ab 267 222A 0 257,-2.6 259,-2.7 -2,-0.4 2,-0.6 -0.992 7.9-167.5-119.6 120.2 -4.1 29.3 1.5 10 39 A L E -ab 268 223A 1 212,-3.4 214,-2.2 -2,-0.5 2,-0.6 -0.944 3.1-167.3-109.8 115.8 -5.8 26.1 0.3 11 40 A L E -ab 269 224A 0 257,-3.0 259,-2.3 -2,-0.6 2,-0.6 -0.915 4.6-165.9-106.7 121.1 -8.7 25.0 2.5 12 41 A I E -ab 270 225A 0 212,-2.3 214,-2.3 -2,-0.6 2,-0.8 -0.924 11.5-161.1-113.8 121.6 -10.0 21.5 1.9 13 42 A S E > - b 0 226A 0 257,-2.5 3,-1.4 -2,-0.6 259,-0.5 -0.858 24.9-136.4 -95.8 108.1 -13.2 20.2 3.2 14 43 A D T 3 S- 0 0 1 212,-1.4 216,-0.6 -2,-0.8 217,-0.1 -0.470 81.5 -0.5 -71.4 118.9 -12.9 16.4 3.0 15 44 A G T 3 S+ 0 0 2 258,-0.5 260,-1.7 -2,-0.4 2,-0.8 0.538 91.1 138.2 89.1 8.4 -16.2 14.9 1.7 16 45 A A < + 0 0 0 -3,-1.4 -1,-0.2 258,-0.2 -3,-0.1 -0.767 23.7 175.9 -97.2 105.8 -18.2 18.1 1.2 17 46 A G > - 0 0 1 -2,-0.8 4,-2.3 258,-0.2 3,-0.2 -0.276 55.6 -82.0 -84.2-173.0 -20.3 18.3 -1.9 18 47 A L H > S+ 0 0 5 294,-0.4 4,-2.8 1,-0.2 641,-0.4 0.843 130.6 58.1 -65.0 -30.3 -22.6 21.3 -2.5 19 48 A S H > S+ 0 0 18 2,-0.2 4,-1.0 1,-0.2 -1,-0.2 0.927 107.8 46.5 -63.7 -42.5 -25.3 19.7 -0.4 20 49 A Q H >> S+ 0 0 1 -3,-0.2 3,-0.8 2,-0.2 4,-0.6 0.932 112.5 50.2 -63.0 -45.6 -22.9 19.7 2.6 21 50 A I H >< S+ 0 0 1 -4,-2.3 3,-1.6 1,-0.2 4,-0.4 0.933 105.9 57.8 -55.2 -43.9 -21.9 23.2 1.9 22 51 A S H >< S+ 0 0 0 -4,-2.8 3,-1.7 1,-0.3 4,-0.3 0.759 89.0 73.0 -61.5 -25.7 -25.6 24.2 1.7 23 52 A S H XX S+ 0 0 0 -4,-1.0 4,-2.7 -3,-0.8 3,-1.5 0.833 87.7 63.0 -62.4 -27.6 -26.1 22.9 5.3 24 53 A T H - 0 0 6 0, 0.0 3,-1.6 0, 0.0 4,-0.1 -0.353 15.7-126.4 -66.9 148.1 -21.8 31.5 6.0 33 62 A N G > S+ 0 0 34 1,-0.3 3,-2.2 2,-0.2 4,-0.3 0.740 102.9 80.2 -66.9 -22.4 -18.4 32.7 5.0 34 63 A Y G > S+ 0 0 0 1,-0.3 3,-1.8 2,-0.2 -1,-0.3 0.794 79.1 71.0 -46.8 -33.2 -18.9 31.0 1.7 35 64 A T G < S+ 0 0 43 -3,-1.6 640,-0.3 1,-0.3 -1,-0.3 0.657 82.0 71.4 -65.9 -14.3 -21.0 34.1 0.7 36 65 A Q G < S+ 0 0 52 -3,-2.2 2,-0.3 -4,-0.1 -1,-0.3 0.708 82.0 93.4 -71.6 -16.1 -17.8 36.2 0.5 37 66 A F < + 0 0 0 -3,-1.8 638,-0.4 -4,-0.3 281,-0.1 -0.567 38.5 167.3 -86.0 137.9 -16.8 34.4 -2.7 38 67 A K + 0 0 112 279,-0.5 636,-1.7 -2,-0.3 2,-0.6 0.717 64.4 71.9-105.8 -42.6 -17.5 35.6 -6.2 39 68 A N E +C 317 0A 22 278,-0.8 278,-2.2 634,-0.2 2,-0.4 -0.663 64.2 171.8 -85.7 118.9 -15.3 33.4 -8.3 40 69 A I E +C 316 0A 1 -2,-0.6 287,-0.7 347,-0.4 347,-0.4 -0.970 7.5 171.1-131.6 136.3 -16.6 29.9 -8.4 41 70 A G E -Cd 315 327A 0 274,-2.5 274,-2.8 -2,-0.4 2,-0.4 -0.755 23.4-128.2-129.6-178.7 -15.6 26.8 -10.4 42 71 A L E -Cd 314 328A 0 285,-2.3 287,-2.4 272,-0.3 2,-0.4 -1.000 16.4-155.1-135.1 137.5 -16.4 23.1 -10.5 43 72 A I E -Cd 313 329A 0 270,-2.7 270,-2.3 -2,-0.4 2,-0.5 -0.918 13.1-138.1-115.7 141.9 -14.0 20.1 -10.5 44 73 A K E - d 0 330A 46 285,-2.1 287,-2.7 -2,-0.4 288,-0.3 -0.832 19.4-166.3 -90.2 128.4 -14.4 16.6 -11.7 45 74 A T + 0 0 0 -2,-0.5 -1,-0.1 265,-0.3 16,-0.1 0.382 34.3 131.3-108.6 4.5 -12.9 14.3 -9.2 46 75 A S - 0 0 11 264,-0.2 5,-0.2 6,-0.1 285,-0.1 -0.179 50.1-130.6 -59.2 150.8 -12.5 10.8 -10.8 47 76 A S - 0 0 13 3,-2.2 22,-0.2 20,-0.1 20,-0.1 -0.211 33.4 -88.3 -91.4-174.2 -9.0 9.2 -10.3 48 77 A S S S+ 0 0 48 20,-2.1 21,-0.2 1,-0.2 3,-0.1 0.709 125.0 16.4 -68.2 -19.1 -6.9 7.6 -13.0 49 78 A R S S+ 0 0 142 19,-0.5 2,-0.4 1,-0.2 -1,-0.2 0.499 119.1 60.6-129.6 -10.7 -8.6 4.2 -12.5 50 79 A E - 0 0 37 1,-0.1 -3,-2.2 4,-0.0 594,-0.2 -0.982 58.1-148.2-133.0 134.1 -11.8 4.8 -10.6 51 80 A D S S+ 0 0 58 593,-1.7 2,-0.6 -2,-0.4 593,-0.2 0.879 98.1 53.1 -63.7 -37.5 -15.0 6.8 -11.3 52 81 A V S S- 0 0 0 591,-2.7 -1,-0.2 -6,-0.1 258,-0.1 -0.890 87.2-147.4-103.1 119.2 -15.3 7.4 -7.5 53 82 A T - 0 0 0 -2,-0.6 2,-0.2 256,-0.2 255,-0.1 -0.356 1.4-141.9 -83.1 158.1 -12.2 8.8 -5.9 54 83 A D > - 0 0 15 -2,-0.1 4,-2.0 -4,-0.1 5,-0.1 -0.605 35.9 -98.3-103.7 176.7 -10.7 8.2 -2.5 55 84 A X H > S+ 0 0 17 -2,-0.2 4,-2.0 2,-0.2 5,-0.2 0.797 117.5 57.0 -66.3 -30.1 -9.1 10.9 -0.4 56 85 A A H > S+ 0 0 0 2,-0.2 4,-2.2 1,-0.2 18,-0.3 0.971 113.8 35.1 -69.5 -55.4 -5.5 9.9 -1.3 57 86 A S H > S+ 0 0 2 1,-0.2 4,-1.8 2,-0.2 -2,-0.2 0.878 117.2 57.1 -65.5 -35.5 -5.8 10.2 -5.1 58 87 A G H X S+ 0 0 0 -4,-2.0 4,-0.8 1,-0.2 -1,-0.2 0.879 112.4 38.6 -62.0 -40.2 -8.1 13.2 -4.6 59 88 A A H X S+ 0 0 0 -4,-2.0 4,-3.0 2,-0.2 5,-0.2 0.810 111.0 58.3 -82.2 -33.3 -5.6 15.2 -2.6 60 89 A T H X>S+ 0 0 0 -4,-2.2 4,-2.4 12,-0.3 6,-0.6 0.847 99.8 59.7 -65.2 -30.6 -2.7 14.0 -4.7 61 90 A A H X5S+ 0 0 1 -4,-1.8 4,-1.2 -5,-0.2 274,-0.5 0.932 112.9 38.8 -59.8 -41.1 -4.5 15.6 -7.7 62 91 A F H <5S+ 0 0 2 -4,-0.8 -2,-0.2 1,-0.2 -1,-0.2 0.910 121.5 41.6 -72.6 -44.6 -4.3 18.9 -5.8 63 92 A S H <5S+ 0 0 0 -4,-3.0 22,-2.6 1,-0.1 23,-0.2 0.781 131.8 19.9 -78.6 -26.1 -0.8 18.5 -4.3 64 93 A C H <5S- 0 0 7 -4,-2.4 -3,-0.2 20,-0.2 -2,-0.2 0.479 94.1-124.1-126.6 -11.3 0.9 17.1 -7.4 65 94 A G S < - 0 0 29 -2,-0.3 3,-2.6 -22,-0.2 6,-0.1 -0.445 56.2 -89.8 -80.5 147.4 -4.9 5.6 -8.4 70 99 A N T 3 S+ 0 0 23 1,-0.3 -1,-0.1 -2,-0.1 3,-0.1 -0.294 116.7 25.1 -51.6 132.1 -7.1 5.4 -5.3 71 100 A A T 3 S+ 0 0 16 1,-0.3 48,-1.5 47,-0.1 -1,-0.3 0.142 89.4 132.6 91.1 -12.8 -4.8 5.1 -2.3 72 101 A A < - 0 0 0 -3,-2.6 2,-0.5 46,-0.2 -1,-0.3 -0.470 41.1-158.8 -69.2 139.0 -1.8 6.7 -3.9 73 102 A I B S-J 116 0B 0 43,-2.1 43,-2.3 -2,-0.2 3,-0.1 -0.934 72.1 -29.0-120.7 102.6 0.0 9.3 -1.8 74 103 A G S S+ 0 0 0 -2,-0.5 8,-1.7 -18,-0.3 2,-0.3 0.792 110.9 111.5 64.5 27.7 2.1 11.7 -3.9 75 104 A V B -K 81 0C 4 6,-0.2 6,-0.2 41,-0.2 -1,-0.2 -0.958 65.0-117.0-130.1 154.6 2.8 9.2 -6.6 76 105 A A > - 0 0 17 4,-2.9 3,-2.5 -2,-0.3 -7,-0.2 -0.167 42.1 -89.7 -81.7 177.8 1.7 8.9 -10.2 77 106 A D T 3 S+ 0 0 60 -10,-0.6 -9,-0.1 1,-0.3 -1,-0.1 0.812 131.5 51.5 -62.6 -28.8 -0.4 6.2 -11.8 78 107 A D T 3 S- 0 0 111 2,-0.1 -1,-0.3 -9,-0.0 3,-0.1 0.266 122.0-111.0 -88.7 11.2 2.7 4.2 -12.6 79 108 A S S < S+ 0 0 47 -3,-2.5 2,-0.3 1,-0.2 -2,-0.1 0.480 71.0 140.8 75.5 7.2 3.8 4.7 -8.9 80 109 A T - 0 0 73 1,-0.1 -4,-2.9 -5,-0.1 -1,-0.2 -0.606 59.1-107.1 -80.1 139.1 6.7 7.0 -9.6 81 110 A A B -K 75 0C 67 -2,-0.3 2,-0.3 -6,-0.2 -6,-0.2 -0.371 36.0-159.5 -65.7 142.5 7.0 9.8 -7.0 82 111 A V - 0 0 32 -8,-1.7 2,-0.2 -2,-0.0 -17,-0.1 -0.906 25.3 -99.0-122.5 153.2 6.1 13.3 -8.3 83 112 A K - 0 0 76 -2,-0.3 2,-0.2 -19,-0.1 -9,-0.0 -0.510 39.7-144.7 -73.1 133.2 7.0 16.7 -6.9 84 113 A S > - 0 0 1 -2,-0.2 4,-2.5 1,-0.1 -20,-0.2 -0.498 26.3-106.4 -93.6 166.8 4.3 18.3 -4.7 85 114 A I H > S+ 0 0 2 -22,-2.6 4,-2.8 1,-0.2 5,-0.2 0.828 121.8 57.4 -58.7 -32.3 3.4 21.9 -4.4 86 115 A V H > S+ 0 0 0 26,-0.2 4,-2.2 2,-0.2 -1,-0.2 0.923 108.9 44.1 -65.0 -42.9 5.1 21.9 -1.0 87 116 A E H > S+ 0 0 48 2,-0.2 4,-1.9 1,-0.2 -2,-0.2 0.897 115.8 47.5 -66.8 -41.5 8.4 20.8 -2.6 88 117 A I H X S+ 0 0 0 -4,-2.5 4,-1.3 2,-0.2 -2,-0.2 0.917 110.2 52.6 -67.0 -43.8 8.0 23.3 -5.4 89 118 A A H ><>S+ 0 0 0 -4,-2.8 5,-2.4 1,-0.2 3,-0.9 0.954 107.6 52.1 -56.7 -48.7 7.2 26.0 -3.0 90 119 A A H ><5S+ 0 0 34 -4,-2.2 3,-1.9 1,-0.3 -1,-0.2 0.903 105.7 54.2 -54.6 -43.0 10.3 25.2 -1.0 91 120 A L H 3<5S+ 0 0 122 -4,-1.9 -1,-0.3 1,-0.3 -2,-0.2 0.795 108.9 50.0 -62.5 -26.9 12.4 25.5 -4.2 92 121 A N T <<5S- 0 0 49 -4,-1.3 -1,-0.3 -3,-0.9 -2,-0.2 0.166 123.4-105.5 -96.4 14.7 10.9 29.0 -4.7 93 122 A N T < 5S+ 0 0 128 -3,-1.9 2,-0.4 1,-0.2 126,-0.4 0.854 71.6 147.2 66.2 35.5 11.8 30.0 -1.2 94 123 A I < - 0 0 13 -5,-2.4 -1,-0.2 -6,-0.1 126,-0.2 -0.891 47.0-127.8-103.3 134.0 8.1 29.8 -0.0 95 124 A K E -e 220 0A 63 124,-2.6 126,-2.6 -2,-0.4 2,-0.3 -0.576 30.0-153.0 -78.7 143.9 7.4 28.8 3.6 96 125 A T E +e 221 0A 0 37,-0.3 39,-2.0 -2,-0.2 40,-1.3 -0.907 18.0 175.6-129.2 148.5 4.9 25.9 3.9 97 126 A G E -ef 222 136A 0 124,-1.9 126,-2.1 -2,-0.3 2,-0.4 -0.996 11.8-161.2-148.6 150.5 2.4 24.5 6.4 98 127 A V E -ef 223 137A 0 38,-1.9 40,-1.6 -2,-0.3 2,-0.4 -1.000 9.9-178.3-133.2 131.9 -0.2 21.8 6.8 99 128 A V E +ef 224 138A 0 124,-2.5 126,-2.5 -2,-0.4 2,-0.3 -0.986 9.2 164.7-129.3 138.9 -3.1 21.7 9.3 100 129 A A E -e 225 0A 0 38,-2.3 40,-0.2 -2,-0.4 126,-0.1 -0.993 39.8-142.5-150.4 154.5 -5.7 18.9 9.7 101 130 A T E S+ 0 0 0 124,-0.8 90,-1.6 -2,-0.3 2,-0.1 0.460 91.8 65.3 -94.7 -4.1 -8.3 17.8 12.2 102 131 A S E S- 0 0 0 1,-0.2 38,-2.6 88,-0.2 -2,-0.2 -0.286 115.7 -59.5 -88.6-173.0 -7.5 14.1 11.4 103 132 A S E > - f 0 140A 7 36,-0.2 3,-1.9 1,-0.1 38,-0.3 -0.483 50.7-121.4 -58.3 147.0 -4.1 12.6 12.2 104 133 A I T 3 S+ 0 0 0 36,-2.5 -1,-0.1 1,-0.3 37,-0.1 0.633 114.1 60.5 -69.9 -11.4 -1.5 14.5 10.3 105 134 A T T 3 S+ 0 0 0 35,-0.1 -1,-0.3 4,-0.1 20,-0.1 0.431 79.8 116.3 -92.2 -1.9 -0.6 11.2 8.6 106 135 A D S <> S- 0 0 20 -3,-1.9 4,-2.6 1,-0.1 5,-0.2 -0.184 84.5 -94.1 -68.4 165.4 -4.1 10.8 7.1 107 136 A A H > S+ 0 0 1 1,-0.2 4,-1.8 2,-0.2 -1,-0.1 0.788 117.0 52.4 -56.3 -39.0 -4.4 10.9 3.3 108 137 A T H 4 S+ 0 0 0 2,-0.2 4,-0.4 1,-0.2 -1,-0.2 0.982 117.5 32.8 -69.5 -59.0 -5.2 14.5 2.8 109 138 A P H >4 S+ 0 0 0 0, 0.0 3,-1.8 0, 0.0 4,-0.5 0.907 115.8 60.7 -61.3 -37.5 -2.3 16.1 4.7 110 139 A A H >X S+ 0 0 0 -4,-2.6 4,-2.9 1,-0.3 3,-1.6 0.873 94.8 61.8 -58.3 -37.9 0.0 13.2 3.8 111 140 A S T 3< S+ 0 0 1 -4,-1.8 -1,-0.3 1,-0.3 -3,-0.1 0.699 101.2 55.2 -62.7 -18.1 -0.4 13.9 0.1 112 141 A F T <4 S+ 0 0 1 -3,-1.8 -1,-0.3 -4,-0.4 -26,-0.2 0.583 126.0 14.5 -91.8 -8.8 1.2 17.4 0.7 113 142 A Y T <4 S+ 0 0 9 -3,-1.6 2,-0.3 -4,-0.5 -2,-0.2 0.528 114.5 60.1-139.0 -7.8 4.4 16.1 2.3 114 143 A A < - 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