==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=14-APR-2010 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER RNA-BINDING PROTEIN 16-MAR-09 2WCT . COMPND 2 MOLECULE: NON-STRUCTURAL PROTEIN 3; . SOURCE 2 ORGANISM_SCIENTIFIC: SARS CORONAVIRUS; . AUTHOR J.TAN,C.VONRHEIN,O.S.SMART,G.BRICOGNE,M.BOLLATI,G.HANSEN, . 1014 10 4 0 4 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 46512.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 638 62.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 144 14.2 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 48 4.7 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 10 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 6 0.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 4 0.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 92 9.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 70 6.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 254 25.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 8 0.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 2 4 0 0 8 0 8 8 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 8 8 8 4 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 4 0 0 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 4 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 389 A K 0 0 124 0, 0.0 122,-2.2 0, 0.0 2,-1.0 0.000 360.0 360.0 360.0 127.2 -8.2 -6.9 -22.2 2 390 A I E -a 123 0A 39 120,-0.2 122,-0.1 1,-0.2 3,-0.1 -0.656 360.0-178.6 -76.8 101.9 -4.6 -5.9 -21.5 3 391 A K E - 0 0 143 120,-1.5 2,-0.3 -2,-1.0 121,-0.2 0.923 64.8 -15.0 -66.6 -46.8 -3.4 -4.8 -25.0 4 392 A A E -a 124 0A 25 119,-1.6 121,-2.3 -3,-0.1 2,-0.5 -0.976 56.0-131.9-155.1 161.7 0.1 -4.0 -23.8 5 393 A C E -a 125 0A 51 -2,-0.3 2,-0.2 119,-0.2 121,-0.2 -0.990 31.4-119.5-122.5 124.3 2.2 -3.4 -20.7 6 394 A I + 0 0 19 119,-2.3 6,-0.2 -2,-0.5 4,-0.1 -0.479 48.7 148.4 -66.0 124.7 4.5 -0.3 -20.6 7 395 A D - 0 0 120 4,-1.3 -1,-0.1 -2,-0.2 5,-0.1 0.352 68.3-105.5-136.6 -3.5 8.2 -1.4 -20.1 8 396 A E S S+ 0 0 145 3,-1.5 2,-0.3 0, 0.0 4,-0.2 0.163 98.4 88.1 85.2 -9.4 10.1 1.3 -22.1 9 397 A V S S- 0 0 108 2,-0.3 3,-0.0 1,-0.0 0, 0.0 -0.867 104.6 -11.2-161.5 125.0 10.7 -1.4 -24.7 10 398 A T S S+ 0 0 121 -2,-0.3 2,-0.4 -3,-0.1 -4,-0.1 -0.827 134.0 44.0-110.2 33.1 8.9 -2.0 -27.0 11 399 A T - 0 0 33 -6,-0.2 -3,-1.5 4,-0.0 -4,-1.3 -0.989 64.2-179.0-136.0 116.1 5.9 0.1 -25.8 12 400 A T > - 0 0 29 -2,-0.4 4,-2.4 -4,-0.2 5,-0.1 -0.788 38.1-117.9-115.0 160.5 6.4 3.6 -24.4 13 401 A L H > S+ 0 0 56 -2,-0.3 4,-1.1 2,-0.2 -1,-0.1 0.875 119.1 47.9 -62.3 -40.5 3.9 6.2 -23.1 14 402 A E H 4 S+ 0 0 136 1,-0.2 4,-0.2 2,-0.2 -1,-0.2 0.838 113.3 49.5 -68.5 -34.6 4.8 8.7 -25.9 15 403 A E H >4 S+ 0 0 96 1,-0.2 3,-0.9 2,-0.2 4,-0.3 0.854 109.5 50.6 -71.4 -39.7 4.3 5.8 -28.4 16 404 A T H >X S+ 0 0 7 -4,-2.4 4,-1.4 1,-0.2 3,-1.1 0.694 90.6 78.4 -74.0 -21.2 0.9 4.7 -26.9 17 405 A K T 3< S+ 0 0 107 -4,-1.1 -1,-0.2 1,-0.3 -2,-0.2 0.687 85.8 62.1 -62.1 -18.2 -0.4 8.3 -27.1 18 406 A F T <4 S+ 0 0 180 -3,-0.9 -1,-0.3 -4,-0.2 -2,-0.2 0.831 107.0 44.8 -73.7 -33.2 -1.0 7.7 -30.9 19 407 A L T <4 S- 0 0 93 -3,-1.1 2,-0.3 -4,-0.3 -2,-0.2 0.787 132.8 -15.0 -78.0 -38.0 -3.4 4.9 -30.0 20 408 A T < - 0 0 13 -4,-1.4 -1,-0.3 1,-0.1 75,-0.1 -0.952 37.1-165.2-165.6 154.7 -5.3 6.8 -27.3 21 409 A N + 0 0 82 -2,-0.3 2,-0.1 73,-0.2 44,-0.1 0.458 61.7 110.7-120.7 -14.4 -5.1 9.8 -25.0 22 410 A K - 0 0 61 72,-0.3 74,-2.0 44,-0.1 2,-0.4 -0.410 53.0-157.1 -64.8 132.8 -7.8 8.7 -22.6 23 411 A L E -bc 66 96A 21 42,-1.4 44,-2.3 72,-0.2 2,-0.6 -0.910 9.8-144.2-118.1 144.8 -6.7 7.7 -19.1 24 412 A L E +bc 67 97A 0 72,-2.1 74,-0.6 -2,-0.4 2,-0.3 -0.919 23.7 179.1-111.9 106.7 -8.4 5.5 -16.6 25 413 A L E -b 68 0A 0 42,-3.0 44,-2.0 -2,-0.6 2,-0.4 -0.730 16.9-139.7-103.4 154.4 -7.9 6.7 -13.0 26 414 A F E +b 69 0A 1 -2,-0.3 8,-3.0 42,-0.2 2,-0.3 -0.943 20.9 177.0-119.7 138.6 -9.3 5.0 -9.8 27 415 A A E -bD 70 33A 1 42,-2.0 44,-1.9 -2,-0.4 6,-0.2 -0.835 22.5-118.5-133.0 168.2 -10.7 6.7 -6.7 28 416 A D - 0 0 40 4,-1.9 3,-0.5 -2,-0.3 27,-0.2 -0.302 45.4 -84.0 -96.6-175.8 -12.3 5.8 -3.4 29 417 A I S S+ 0 0 29 1,-0.2 26,-2.6 42,-0.1 928,-0.1 0.898 128.5 52.2 -56.0 -45.9 -15.8 6.6 -2.0 30 418 A N S S- 0 0 83 24,-0.2 -1,-0.2 23,-0.1 23,-0.1 0.759 119.9-109.0 -63.1 -29.7 -14.7 10.0 -0.7 31 419 A G S S+ 0 0 4 -3,-0.5 2,-0.4 1,-0.3 -2,-0.1 0.399 70.7 140.5 111.5 3.0 -13.3 10.9 -4.2 32 420 A K - 0 0 138 1,-0.1 -4,-1.9 18,-0.0 -1,-0.3 -0.639 51.7-121.1 -79.5 129.0 -9.6 10.7 -3.4 33 421 A L B -D 27 0A 6 -2,-0.4 -6,-0.3 -6,-0.2 -1,-0.1 -0.355 28.4-100.8 -70.6 150.8 -7.5 9.2 -6.2 34 422 A Y > - 0 0 36 -8,-3.0 4,-2.0 1,-0.1 5,-0.2 -0.299 31.4-111.2 -69.4 153.2 -5.4 6.1 -5.7 35 423 A H H > S+ 0 0 98 67,-0.4 4,-1.4 1,-0.2 3,-0.1 0.891 118.1 46.6 -51.5 -49.1 -1.6 6.4 -5.2 36 424 A D H > S+ 0 0 20 1,-0.2 4,-0.5 66,-0.2 -1,-0.2 0.850 116.1 45.8 -62.9 -39.2 -0.8 4.8 -8.6 37 425 A S H 4 S+ 0 0 1 -11,-0.2 4,-0.2 1,-0.2 -1,-0.2 0.700 106.5 59.2 -78.5 -23.0 -3.4 6.9 -10.4 38 426 A Q H >< S+ 0 0 84 -4,-2.0 3,-0.9 1,-0.2 -2,-0.2 0.834 102.5 53.3 -73.0 -35.1 -2.3 10.1 -8.6 39 427 A N H 3< S+ 0 0 97 -4,-1.4 -1,-0.2 1,-0.2 -2,-0.2 0.718 96.7 67.5 -71.5 -24.8 1.3 9.7 -10.1 40 428 A M T 3< S+ 0 0 25 -4,-0.5 -1,-0.2 2,-0.0 -2,-0.2 0.632 83.1 100.3 -68.9 -18.5 -0.2 9.5 -13.6 41 429 A L < - 0 0 27 -3,-0.9 2,-0.1 -4,-0.2 -3,-0.0 -0.429 54.6-164.8 -75.2 145.8 -1.3 13.1 -13.4 42 430 A R S S- 0 0 203 -2,-0.1 -1,-0.1 0, 0.0 -3,-0.1 -0.573 70.7 -62.3-129.4 64.2 0.6 16.0 -15.0 43 431 A G S S+ 0 0 86 1,-0.2 2,-0.2 -2,-0.1 -2,-0.0 0.820 84.3 160.1 60.7 41.0 -0.8 19.1 -13.4 44 432 A E - 0 0 73 1,-0.1 2,-0.8 2,-0.0 -1,-0.2 -0.534 51.7 -91.3 -90.3 157.0 -4.4 18.7 -14.6 45 433 A D + 0 0 112 -2,-0.2 3,-0.4 1,-0.1 -1,-0.1 -0.577 41.7 176.9 -71.8 105.4 -7.5 20.3 -13.0 46 434 A M + 0 0 45 -2,-0.8 4,-0.4 1,-0.2 3,-0.2 -0.054 51.6 102.8-100.7 30.6 -8.8 17.8 -10.5 47 435 A S >> + 0 0 52 1,-0.2 4,-0.9 2,-0.2 3,-0.6 0.752 67.7 67.5 -82.5 -27.6 -11.7 20.0 -9.3 48 436 A F T 34 S+ 0 0 32 -3,-0.4 4,-0.3 1,-0.2 -1,-0.2 0.706 96.2 56.7 -64.4 -25.4 -14.4 18.2 -11.2 49 437 A L T 34 S+ 0 0 8 -3,-0.2 3,-0.2 1,-0.2 -1,-0.2 0.738 108.3 44.0 -79.6 -28.8 -13.9 15.1 -9.0 50 438 A E T <4 S+ 0 0 129 -3,-0.6 3,-0.4 -4,-0.4 -2,-0.2 0.591 98.7 75.4 -91.4 -12.6 -14.6 16.9 -5.7 51 439 A K S < S+ 0 0 162 -4,-0.9 -1,-0.2 1,-0.2 -2,-0.2 0.697 106.8 30.1 -71.2 -23.2 -17.6 18.8 -7.2 52 440 A D S S+ 0 0 51 -4,-0.3 -1,-0.2 -3,-0.2 -2,-0.1 0.248 97.0 166.2-122.1 11.6 -19.8 15.7 -7.0 53 441 A A + 0 0 31 -3,-0.4 -23,-0.1 -4,-0.1 2,-0.1 -0.396 40.7 58.2 -72.4 147.4 -18.3 13.8 -4.0 54 442 A P S S- 0 0 54 0, 0.0 2,-0.2 0, 0.0 -24,-0.2 0.535 73.4-173.3 -81.0 156.5 -19.2 11.5 -2.2 55 443 A Y - 0 0 6 -26,-2.6 2,-0.2 -27,-0.2 -24,-0.1 -0.726 20.9-123.6-115.2 164.0 -19.7 8.7 -4.7 56 444 A M > - 0 0 3 -2,-0.2 3,-1.7 867,-0.1 2,-0.3 -0.628 47.9 -81.2 -98.8 160.4 -21.0 5.1 -4.8 57 445 A V T 3 S+ 0 0 12 926,-0.8 928,-0.3 1,-0.2 14,-0.2 -0.499 119.4 28.3 -66.6 124.0 -18.9 2.1 -6.0 58 446 A G T 3 S+ 0 0 2 12,-2.5 2,-0.4 1,-0.3 -1,-0.2 0.410 81.6 147.3 103.3 4.2 -19.0 2.0 -9.8 59 447 A D < - 0 0 21 -3,-1.7 11,-1.8 11,-0.1 2,-0.5 -0.590 35.0-152.1 -74.5 125.3 -19.5 5.7 -10.3 60 448 A V E -E 69 0A 2 207,-0.5 2,-0.4 -2,-0.4 9,-0.2 -0.904 11.8-173.0-105.7 123.1 -17.8 6.9 -13.6 61 449 A I E -E 68 0A 8 7,-3.2 7,-3.3 -2,-0.5 2,-0.4 -0.966 4.1-173.5-118.4 126.6 -16.6 10.6 -13.8 62 450 A T E +E 67 0A 75 -2,-0.4 2,-0.3 5,-0.2 5,-0.2 -0.976 17.8 172.8-127.3 135.6 -15.3 12.1 -17.1 63 451 A S E > S-E 66 0A 57 3,-2.5 3,-1.2 -2,-0.4 2,-0.6 -0.769 72.3 -61.3-138.5 84.5 -13.6 15.4 -17.9 64 452 A G T 3 S- 0 0 68 -2,-0.3 -42,-0.1 1,-0.3 0, 0.0 -0.658 121.9 -10.1 77.8-113.1 -12.4 15.3 -21.5 65 453 A D T 3 S+ 0 0 69 -2,-0.6 -42,-1.4 -44,-0.1 2,-0.4 0.760 115.6 93.7 -87.9 -28.1 -9.8 12.5 -21.8 66 454 A I E < -bE 23 63A 21 -3,-1.2 -3,-2.5 -44,-0.2 2,-0.4 -0.552 55.0-176.0 -71.7 119.0 -9.5 11.9 -18.0 67 455 A T E -bE 24 62A 3 -44,-2.3 -42,-3.0 -2,-0.4 2,-0.4 -0.979 2.1-169.5-121.0 128.1 -11.9 9.1 -16.9 68 456 A C E -bE 25 61A 0 -7,-3.3 -7,-3.2 -2,-0.4 2,-0.4 -0.967 5.1-170.2-123.3 138.5 -12.3 8.1 -13.2 69 457 A V E -bE 26 60A 0 -44,-2.0 -42,-2.0 -2,-0.4 2,-0.7 -0.985 23.5-130.3-128.5 133.4 -14.1 5.2 -11.6 70 458 A V E -b 27 0A 0 -11,-1.8 -12,-2.5 -2,-0.4 -42,-0.2 -0.762 34.6-164.8 -82.8 114.9 -14.8 4.6 -8.0 71 459 A I - 0 0 3 -44,-1.9 -42,-0.1 -2,-0.7 12,-0.1 -0.688 21.9 -98.8-102.3 155.0 -13.6 1.0 -7.2 72 460 A P - 0 0 2 0, 0.0 2,-0.6 0, 0.0 -1,-0.1 -0.414 32.8-123.7 -74.2 152.5 -14.3 -1.3 -4.3 73 461 A S > - 0 0 32 1,-0.1 5,-3.3 -2,-0.1 3,-0.4 -0.863 12.0-160.3 -99.0 120.8 -11.8 -1.5 -1.4 74 462 A K T > 5S+ 0 0 35 -2,-0.6 3,-1.6 3,-0.3 768,-0.2 0.872 95.5 54.6 -66.0 -38.0 -10.5 -5.1 -0.6 75 463 A K T 3 5S+ 0 0 97 1,-0.3 -1,-0.2 29,-0.1 768,-0.1 0.705 108.7 50.5 -67.2 -20.9 -9.4 -4.1 2.9 76 464 A A T 3 5S- 0 0 60 -3,-0.4 -1,-0.3 765,-0.1 -2,-0.2 0.273 137.8 -86.4 -97.7 7.0 -13.0 -2.8 3.4 77 465 A G T < 5S+ 0 0 15 -3,-1.6 -3,-0.3 1,-0.2 -2,-0.1 0.311 74.0 156.0 107.4 -6.9 -14.3 -6.2 2.1 78 466 A G < - 0 0 8 -5,-3.3 -1,-0.2 762,-0.1 2,-0.2 -0.132 29.2-142.6 -54.5 141.5 -14.5 -5.7 -1.7 79 467 A T > - 0 0 31 1,-0.1 4,-1.6 28,-0.0 5,-0.1 -0.590 22.7-113.4-105.2 168.3 -14.3 -8.7 -3.9 80 468 A T H > S+ 0 0 43 1,-0.2 4,-2.3 2,-0.2 5,-0.1 0.867 120.4 51.3 -64.2 -39.5 -12.7 -9.5 -7.3 81 469 A E H > S+ 0 0 110 1,-0.2 4,-1.5 2,-0.2 -1,-0.2 0.825 107.7 53.2 -65.8 -37.5 -16.2 -9.8 -8.9 82 470 A M H > S+ 0 0 8 2,-0.2 4,-1.3 1,-0.2 -2,-0.2 0.844 110.5 46.1 -67.2 -40.2 -17.1 -6.4 -7.4 83 471 A L H X S+ 0 0 0 -4,-1.6 4,-1.9 2,-0.2 3,-0.2 0.921 111.7 51.4 -67.9 -46.9 -14.1 -4.7 -9.0 84 472 A S H X S+ 0 0 8 -4,-2.3 4,-1.0 1,-0.2 -2,-0.2 0.819 109.9 50.9 -59.5 -33.9 -14.7 -6.4 -12.3 85 473 A R H < S+ 0 0 61 -4,-1.5 -1,-0.2 2,-0.2 4,-0.2 0.823 108.8 49.6 -74.1 -34.3 -18.3 -5.2 -12.2 86 474 A A H >< S+ 0 0 4 -4,-1.3 3,-0.7 -3,-0.2 -2,-0.2 0.805 108.9 53.7 -73.3 -31.5 -17.2 -1.6 -11.5 87 475 A L H >< S+ 0 0 0 -4,-1.9 3,-1.1 1,-0.2 -2,-0.2 0.776 100.5 60.6 -71.5 -28.9 -14.8 -1.8 -14.4 88 476 A K T 3< S+ 0 0 101 -4,-1.0 -1,-0.2 1,-0.2 -2,-0.2 0.581 93.7 65.7 -74.0 -11.9 -17.7 -2.9 -16.7 89 477 A K T < S+ 0 0 58 -3,-0.7 -1,-0.2 -4,-0.2 -2,-0.2 0.574 81.3 94.9 -84.5 -12.4 -19.4 0.5 -15.9 90 478 A V S < S- 0 0 4 -3,-1.1 2,-0.2 -4,-0.2 31,-0.1 -0.624 92.1-103.6 -78.6 134.7 -16.5 2.3 -17.8 91 479 A P - 0 0 68 0, 0.0 2,-0.3 0, 0.0 -2,-0.1 -0.460 43.5 -98.6 -67.0 128.8 -17.4 3.0 -21.4 92 480 A V S S+ 0 0 95 -2,-0.2 2,-0.2 -4,-0.1 29,-0.1 -0.294 74.7 110.8 -53.7 109.4 -15.7 0.6 -23.9 93 481 A D S S- 0 0 50 -2,-0.3 29,-1.8 29,-0.1 2,-0.5 -0.881 71.6 -70.4-160.8-173.6 -12.6 2.3 -25.3 94 482 A E E - f 0 122A 74 -2,-0.2 2,-0.3 27,-0.2 -72,-0.3 -0.848 55.3-179.4 -96.4 127.3 -8.7 2.3 -25.4 95 483 A Y E - f 0 123A 3 27,-2.5 29,-2.5 -2,-0.5 2,-0.6 -0.976 31.9-139.8-133.7 147.7 -7.2 3.5 -22.1 96 484 A I E +cf 23 124A 1 -74,-2.0 -72,-2.1 -2,-0.3 2,-0.2 -0.918 48.6 151.2-101.9 121.3 -3.8 4.1 -20.5 97 485 A T E -cf 24 125A 0 27,-2.3 29,-3.1 -2,-0.6 2,-0.2 -0.781 41.5-124.2-142.0-178.4 -4.1 3.0 -16.9 98 486 A T - 0 0 5 -74,-0.6 30,-0.0 -2,-0.2 -61,-0.0 -0.590 52.3 -74.6-117.2-179.3 -2.4 1.5 -13.8 99 487 A Y - 0 0 12 27,-0.3 3,-0.3 -2,-0.2 2,-0.2 -0.712 55.7-118.8 -83.8 127.3 -3.3 -1.6 -11.8 100 488 A P S S+ 0 0 0 0, 0.0 15,-0.1 0, 0.0 12,-0.1 -0.474 80.5 16.5 -74.7 140.8 -6.5 -1.0 -9.7 101 489 A G S S- 0 0 5 1,-0.2 6,-1.0 -2,-0.2 2,-0.4 0.966 81.0-152.7 70.1 65.6 -6.5 -1.2 -5.9 102 490 A Q - 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