==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=19-DEC-2009 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER IMMUNE SYSTEM 16-NOV-04 1WT5 . COMPND 2 MOLECULE: ANTI EGFR ANTIBODY FV REGION; . SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS; . AUTHOR K.MAKABE,K.TSUMOTO,R.ASANO,H.KONDO,I.KUMAGAI . 452 4 4 4 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 19403.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 306 67.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 14 3.1 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 174 38.5 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 7 1.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 3 0.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 1 0.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 55 12.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 36 8.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 8 1.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 3 0.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 3 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 5 8 5 1 13 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 3 1 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 A Q 0 0 187 0, 0.0 2,-0.3 0, 0.0 26,-0.0 0.000 360.0 360.0 360.0 144.2 8.8 33.1 -18.3 2 2 A V - 0 0 26 24,-0.1 2,-0.4 22,-0.0 24,-0.3 -0.715 360.0-164.4 -94.3 145.9 7.4 34.3 -15.0 3 3 A Q E -A 25 0A 103 22,-3.3 22,-1.5 -2,-0.3 2,-0.6 -0.998 13.6-166.5-134.1 131.8 9.7 34.7 -12.1 4 4 A L E -A 24 0A 1 104,-0.5 106,-0.7 -2,-0.4 2,-0.6 -0.955 18.4-170.0-114.6 106.8 8.7 35.1 -8.4 5 5 A V E -A 23 0A 57 18,-3.4 18,-2.6 -2,-0.6 2,-0.1 -0.888 1.4-164.7-110.0 116.1 11.9 36.3 -6.6 6 6 A Q E -A 22 0A 5 -2,-0.6 16,-0.2 16,-0.2 2,-0.1 -0.329 26.5 -89.6 -90.3 171.0 11.9 36.3 -2.8 7 7 A S - 0 0 22 14,-1.3 -1,-0.1 1,-0.1 3,-0.1 -0.336 52.7 -86.1 -77.4 160.1 14.2 38.0 -0.3 8 8 A G - 0 0 15 381,-0.1 -1,-0.1 1,-0.1 13,-0.1 -0.139 55.8 -73.8 -67.7 161.7 17.4 36.4 1.1 9 9 A A - 0 0 26 102,-0.1 2,-0.3 1,-0.1 105,-0.2 0.015 51.3-161.2 -50.5 157.9 17.6 34.1 4.1 10 10 A E B -d 114 0B 49 103,-2.6 105,-2.0 -3,-0.1 2,-0.4 -0.994 15.1-146.7-147.4 150.4 17.3 35.6 7.5 11 11 A V - 0 0 70 -2,-0.3 2,-0.4 103,-0.2 103,-0.0 -0.970 23.6-168.3-119.0 131.4 18.1 34.7 11.1 12 12 A K - 0 0 55 103,-3.3 3,-0.1 -2,-0.4 6,-0.1 -0.942 19.3-129.6-129.8 150.2 15.8 35.8 13.9 13 13 A K > - 0 0 125 -2,-0.4 3,-2.6 1,-0.1 73,-0.3 -0.635 49.3 -84.5 -89.0 144.5 15.8 36.0 17.7 14 14 A P T 3 S+ 0 0 116 0, 0.0 73,-0.2 0, 0.0 -1,-0.1 -0.167 117.9 19.9 -49.6 138.9 12.8 34.5 19.6 15 15 A G T 3 S+ 0 0 54 71,-3.5 70,-0.7 1,-0.3 72,-0.1 0.170 101.2 116.0 86.1 -20.1 10.0 37.1 19.8 16 16 A A < - 0 0 20 -3,-2.6 70,-3.1 69,-0.2 2,-0.3 -0.114 60.4-124.2 -73.6 177.0 11.4 39.0 16.9 17 17 A S - 0 0 51 67,-0.2 2,-0.4 68,-0.2 67,-0.2 -0.932 13.4-152.8-126.6 151.5 9.5 39.5 13.6 18 18 A V E - B 0 83A 3 65,-1.3 65,-2.2 -2,-0.3 2,-0.6 -0.976 1.6-157.7-126.8 138.7 10.5 38.8 9.9 19 19 A K E - B 0 82A 92 -2,-0.4 2,-0.5 63,-0.2 63,-0.2 -0.926 13.1-167.9-118.3 106.2 9.3 40.5 6.7 20 20 A V E - B 0 81A 1 61,-2.9 61,-1.9 -2,-0.6 2,-0.2 -0.815 6.1-153.4 -99.2 130.3 9.7 38.4 3.6 21 21 A S E - B 0 80A 31 -2,-0.5 -14,-1.3 59,-0.2 2,-0.3 -0.619 9.7-167.1-101.9 159.8 9.2 39.9 0.1 22 22 A a E -AB 6 79A 0 57,-1.9 57,-2.1 -16,-0.2 2,-0.5 -0.803 5.7-167.8-148.1 97.5 8.2 38.3 -3.2 23 23 A K E -AB 5 78A 103 -18,-2.6 -18,-3.4 -2,-0.3 2,-0.3 -0.778 11.0-160.3 -92.0 129.6 8.7 40.3 -6.4 24 24 A A E +A 4 0A 3 53,-2.8 2,-0.3 -2,-0.5 -20,-0.2 -0.764 16.8 162.5-109.4 153.6 6.9 38.9 -9.5 25 25 A S E +A 3 0A 63 -22,-1.5 -22,-3.3 -2,-0.3 3,-0.1 -0.957 49.6 57.4-164.0 151.2 7.5 39.5 -13.2 26 26 A G S S+ 0 0 63 1,-0.3 2,-0.2 -2,-0.3 -24,-0.1 0.069 104.7 44.1 110.9 -23.7 6.6 37.8 -16.5 27 27 A Y S S- 0 0 38 -24,-0.2 2,-1.3 -26,-0.0 -1,-0.3 -0.805 105.5 -70.9-139.5-179.5 2.9 38.0 -16.0 28 28 A T > - 0 0 89 -2,-0.2 3,-2.2 1,-0.2 4,-0.2 -0.666 43.5-161.8 -83.4 95.5 0.2 40.5 -14.8 29 29 A F G > S+ 0 0 9 -2,-1.3 3,-0.9 1,-0.3 25,-0.2 0.775 83.2 54.7 -45.1 -42.3 1.0 40.7 -11.0 30 30 A T G 3 S+ 0 0 46 1,-0.2 24,-0.7 23,-0.2 -1,-0.3 0.498 98.8 63.1 -77.5 -3.0 -2.4 42.2 -10.1 31 31 A S G < S+ 0 0 49 -3,-2.2 2,-0.3 22,-0.1 -1,-0.2 0.316 94.6 61.1-106.8 11.8 -4.5 39.5 -11.7 32 32 A Y S < S- 0 0 79 -3,-0.9 2,-0.2 -4,-0.2 22,-0.2 -0.988 81.9-109.8-137.8 146.4 -3.5 36.4 -9.7 33 33 A W - 0 0 81 66,-0.4 66,-1.7 -2,-0.3 2,-0.5 -0.506 27.2-147.0 -75.2 142.7 -3.7 35.4 -6.1 34 34 A M E -EF 51 98B 2 17,-2.9 17,-1.9 64,-0.2 2,-0.3 -0.945 17.8-172.3-112.8 120.3 -0.5 35.2 -4.1 35 35 A H E -EF 50 97B 5 62,-3.1 62,-2.3 -2,-0.5 2,-0.5 -0.785 17.1-143.0-112.7 156.9 -0.2 32.6 -1.4 36 36 A W E +EF 49 96B 0 13,-2.1 12,-2.7 -2,-0.3 13,-0.9 -0.978 22.3 175.0-118.9 126.4 2.4 31.9 1.3 37 37 A V E -EF 47 95B 1 58,-2.0 58,-2.8 -2,-0.5 2,-0.4 -0.965 14.9-149.3-126.5 144.5 3.2 28.3 2.2 38 38 A R E -EF 46 94B 6 8,-2.2 8,-1.5 -2,-0.4 2,-0.8 -0.918 12.7-145.1-123.3 150.4 5.9 27.2 4.5 39 39 A Q E +EF 45 93B 29 54,-2.7 54,-1.8 -2,-0.4 6,-0.2 -0.882 36.2 170.4-108.3 92.2 8.1 24.1 4.8 40 40 A A > - 0 0 6 4,-1.3 3,-1.2 -2,-0.8 4,-0.3 -0.872 43.0 -82.5-112.1 144.5 8.5 23.6 8.5 41 41 A P T 3 S- 0 0 59 0, 0.0 -1,-0.1 0, 0.0 50,-0.0 -0.062 111.9 -3.5 -42.3 123.9 10.0 20.6 10.4 42 42 A G T 3 S+ 0 0 77 1,-0.1 295,-0.0 295,-0.1 -3,-0.0 0.899 123.1 78.7 55.1 43.5 7.5 17.7 10.8 43 43 A Q S < S- 0 0 92 -3,-1.2 -3,-0.1 294,-0.1 -5,-0.1 0.175 74.1-161.8-159.2 8.3 4.7 19.8 9.2 44 44 A G - 0 0 13 -4,-0.3 -4,-1.3 293,-0.1 2,-0.4 -0.066 61.9 -13.4 39.5-120.1 5.7 19.4 5.5 45 45 A L E -E 39 0B 4 291,-0.5 290,-0.5 -6,-0.2 2,-0.4 -0.894 68.1-180.0-111.2 140.7 3.9 22.2 3.7 46 46 A E E -E 38 0B 16 -8,-1.5 -8,-2.2 -2,-0.4 2,-0.1 -0.992 28.3-120.5-143.9 134.7 1.2 24.3 5.2 47 47 A W E -E 37 0B 21 286,-0.4 -10,-0.2 -2,-0.4 3,-0.1 -0.463 25.0-178.2 -69.7 142.3 -0.8 27.2 3.7 48 48 A M E - 0 0 0 -12,-2.7 13,-2.2 1,-0.5 2,-0.3 0.722 51.4 -71.3-113.6 -33.0 -0.5 30.4 5.7 49 49 A G E -EG 36 60B 0 -13,-0.9 -13,-2.1 11,-0.3 -1,-0.5 -0.947 42.9-125.6 162.0-173.5 -2.7 32.8 3.8 50 50 A N E -EG 35 59B 16 9,-2.4 9,-1.7 -2,-0.3 2,-0.3 -0.958 7.5-154.6-156.6 170.4 -3.0 34.7 0.6 51 51 A I E -EG 34 58B 1 -17,-1.9 -17,-2.9 -2,-0.3 7,-0.2 -0.990 18.8-130.8-151.7 149.0 -3.5 38.2 -0.9 52 52 A Y >> - 0 0 68 5,-2.2 4,-3.7 -2,-0.3 3,-1.0 -0.906 13.3-154.7-106.0 108.4 -4.9 39.6 -4.2 53 53 A P T 34 S+ 0 0 0 0, 0.0 -23,-0.2 0, 0.0 21,-0.2 0.880 93.7 54.8 -47.3 -41.6 -2.4 42.1 -5.6 54 54 A G T 34 S+ 0 0 40 -24,-0.7 -24,-0.1 -25,-0.2 -23,-0.1 0.815 125.3 18.8 -65.0 -33.2 -5.3 43.8 -7.4 55 55 A S T <4 S- 0 0 88 -3,-1.0 -1,-0.2 2,-0.2 3,-0.1 0.539 89.2-133.2-114.9 -11.8 -7.5 44.4 -4.4 56 56 A G < + 0 0 21 -4,-3.7 2,-0.1 1,-0.3 14,-0.1 0.499 56.8 148.0 70.2 0.0 -5.1 44.0 -1.5 57 57 A G - 0 0 30 -5,-0.4 -5,-2.2 -6,-0.1 -1,-0.3 -0.441 27.2-167.1 -69.5 143.3 -7.7 41.8 0.0 58 58 A T E -G 51 0B 47 -7,-0.2 2,-0.3 -3,-0.1 -7,-0.2 -0.760 23.0-162.5-129.5 174.5 -6.3 39.0 2.2 59 59 A N E -G 50 0B 87 -9,-1.7 -9,-2.4 -2,-0.2 2,-0.4 -0.954 13.1-164.7-154.5 134.6 -7.1 35.7 4.0 60 60 A Y E -G 49 0B 41 -2,-0.3 -11,-0.3 -11,-0.2 5,-0.1 -0.988 25.1-116.7-130.7 135.3 -5.1 34.1 6.8 61 61 A A >> - 0 0 12 -13,-2.2 3,-1.7 -2,-0.4 4,-1.4 -0.361 32.4-119.9 -62.0 147.2 -5.2 30.6 8.2 62 62 A E T 34 S+ 0 0 166 1,-0.3 4,-0.5 2,-0.2 -1,-0.1 0.843 108.8 71.5 -59.7 -33.0 -6.2 30.8 11.9 63 63 A K T 34 S+ 0 0 107 1,-0.2 -1,-0.3 2,-0.1 -2,-0.1 0.698 118.4 17.9 -56.6 -20.4 -2.9 29.1 12.9 64 64 A F T X> S+ 0 0 2 -3,-1.7 4,-2.8 -16,-0.2 3,-2.5 0.420 91.5 105.1-130.7 -4.0 -1.1 32.4 12.0 65 65 A K T 3< S+ 0 0 126 -4,-1.4 -2,-0.1 1,-0.3 -3,-0.1 0.713 82.7 53.7 -55.9 -21.3 -3.8 35.1 11.8 66 66 A N T 34 S+ 0 0 105 -4,-0.5 -1,-0.3 1,-0.1 -3,-0.1 0.448 122.2 26.9 -93.6 0.6 -2.6 36.5 15.2 67 67 A R T <4 S+ 0 0 8 -3,-2.5 17,-2.8 16,-0.0 -2,-0.2 0.565 106.4 75.4-131.8 -23.7 1.0 36.9 14.0 68 68 A V E < -C 83 0A 5 -4,-2.8 2,-0.3 15,-0.2 15,-0.2 -0.493 45.6-178.0 -95.4 165.9 1.0 37.3 10.2 69 69 A T E -C 82 0A 71 13,-0.7 13,-2.2 -2,-0.2 2,-0.4 -0.914 9.8-158.3-159.7 130.1 0.2 40.2 7.9 70 70 A M E +C 81 0A 5 -2,-0.3 2,-0.3 11,-0.2 11,-0.2 -0.935 20.0 157.1-119.1 142.9 0.2 40.2 4.1 71 71 A T E -C 80 0A 57 9,-1.7 9,-2.4 -2,-0.4 2,-0.3 -0.870 18.2-152.3-149.0 177.7 0.5 43.3 1.9 72 72 A R E -C 79 0A 91 -2,-0.3 2,-0.4 7,-0.3 7,-0.3 -0.993 13.4-144.6-158.3 159.8 1.6 44.3 -1.6 73 73 A D E > > -C 78 0A 57 5,-3.0 5,-1.9 -2,-0.3 3,-1.1 -0.913 8.4-171.4-131.7 103.7 2.9 47.1 -3.8 74 74 A T T 3 5S+ 0 0 64 -2,-0.4 3,-0.3 1,-0.2 -44,-0.1 0.686 83.8 65.5 -68.4 -19.4 1.6 47.1 -7.3 75 75 A S T 3 5S+ 0 0 100 1,-0.2 -1,-0.2 3,-0.1 -2,-0.0 0.757 118.0 25.3 -74.9 -23.0 4.1 49.8 -8.3 76 76 A I T < 5S- 0 0 71 -3,-1.1 -1,-0.2 2,-0.2 -2,-0.2 0.129 106.2-121.4-124.7 18.7 6.9 47.4 -7.8 77 77 A S T 5 + 0 0 39 -3,-0.3 -53,-2.8 1,-0.2 2,-0.4 0.867 65.3 137.7 40.4 56.4 5.0 44.1 -8.3 78 78 A T E < -BC 23 73A 8 -5,-1.9 -5,-3.0 -55,-0.2 2,-0.3 -0.985 39.7-161.5-136.4 143.2 5.9 42.8 -4.8 79 79 A A E -BC 22 72A 0 -57,-2.1 -57,-1.9 -2,-0.4 2,-0.3 -0.880 12.6-158.2-117.7 150.0 4.1 41.0 -2.0 80 80 A Y E -BC 21 71A 50 -9,-2.4 -9,-1.7 -2,-0.3 2,-0.4 -0.952 10.1-166.5-132.3 150.7 5.2 40.8 1.6 81 81 A M E -BC 20 70A 1 -61,-1.9 -61,-2.9 -2,-0.3 2,-0.5 -0.979 6.4-165.3-138.4 120.6 4.5 38.5 4.6 82 82 A E E -BC 19 69A 60 -13,-2.2 2,-0.7 -2,-0.4 -13,-0.7 -0.937 2.3-165.6-108.6 122.0 5.5 39.5 8.2 83 83 A L E -BC 18 68A 0 -65,-2.2 -65,-1.3 -2,-0.5 3,-0.2 -0.887 19.2-162.1-107.8 101.2 5.5 36.7 10.8 84 84 A S + 0 0 42 -17,-2.8 -67,-0.2 -2,-0.7 -68,-0.1 -0.370 61.4 24.0 -84.4 161.6 5.6 38.4 14.2 85 85 A R S S- 0 0 126 -70,-0.7 -1,-0.2 -2,-0.1 -69,-0.2 0.962 85.7-160.1 50.8 60.8 6.6 37.0 17.6 86 86 A L - 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