==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=20-DEC-2009 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER CONTRACTILE PROTEIN 13-MAY-05 1X44 . COMPND 2 MOLECULE: MYOSIN-BINDING PROTEIN C, SLOW-TYPE; . SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS; . AUTHOR X.-R.QIN,C.KUROSAKI,F.HAYASHI,M.YOSHIDA,S.YOKOYAMA,RIKEN . 103 1 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 6767.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 67 65.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 5 4.9 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 33 32.0 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 1 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 3 2.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 22 21.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 6 5.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 1 1.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 1 1 0 1 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 A G 0 0 130 0, 0.0 2,-1.3 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 -92.9 -25.3 -2.7 13.4 2 2 A S + 0 0 134 1,-0.0 2,-0.4 2,-0.0 31,-0.0 -0.681 360.0 140.7 -89.1 88.8 -23.5 -3.9 10.3 3 3 A S + 0 0 120 -2,-1.3 2,-0.3 0, 0.0 -1,-0.0 -0.821 18.9 134.4-134.3 94.5 -25.5 -2.2 7.5 4 4 A G - 0 0 57 -2,-0.4 -2,-0.0 1,-0.2 29,-0.0 -0.967 59.2 -70.9-140.8 156.6 -23.5 -0.9 4.6 5 5 A S - 0 0 68 -2,-0.3 -1,-0.2 1,-0.0 4,-0.1 0.117 53.7-105.7 -38.1 156.9 -23.6 -0.9 0.8 6 6 A S S S+ 0 0 119 2,-0.1 79,-0.1 78,-0.0 78,-0.1 0.981 100.8 45.0 -52.5 -74.7 -22.7 -4.2 -0.9 7 7 A G S S- 0 0 17 77,-0.1 2,-0.4 1,-0.1 78,-0.1 0.101 96.5 -96.5 -61.6-177.9 -19.2 -3.4 -2.1 8 8 A I - 0 0 7 24,-0.1 2,-0.4 78,-0.1 77,-0.4 -0.858 29.1-156.1-109.1 141.8 -16.6 -1.7 -0.0 9 9 A M - 0 0 80 -2,-0.4 2,-1.1 -4,-0.1 23,-0.4 -0.959 21.3-123.5-119.9 132.2 -15.7 2.0 -0.1 10 10 A V - 0 0 20 -2,-0.4 21,-0.3 21,-0.2 3,-0.1 -0.593 27.2-178.5 -75.1 99.4 -12.4 3.6 0.9 11 11 A T S S+ 0 0 86 -2,-1.1 2,-0.6 19,-0.3 -1,-0.2 0.923 74.8 42.0 -63.9 -45.8 -13.3 6.0 3.6 12 12 A K B S-A 30 0A 57 18,-1.3 18,-1.0 -3,-0.1 -1,-0.2 -0.913 81.3-150.5-109.7 113.7 -9.7 7.2 4.1 13 13 A Q - 0 0 129 -2,-0.6 15,-0.1 16,-0.1 75,-0.1 -0.548 31.2 -93.8 -82.1 145.5 -7.7 7.7 0.9 14 14 A L - 0 0 15 -2,-0.2 2,-0.4 65,-0.1 75,-0.2 -0.127 39.6-140.7 -54.3 152.1 -3.9 7.2 0.9 15 15 A E - 0 0 131 13,-0.3 13,-0.1 -3,-0.1 75,-0.1 -0.955 28.7 -88.5-122.9 139.5 -1.8 10.3 1.5 16 16 A D - 0 0 101 -2,-0.4 2,-0.4 73,-0.1 76,-0.2 -0.104 47.0-159.9 -43.2 130.2 1.5 11.3 -0.1 17 17 A T E -d 92 0B 35 74,-1.8 76,-1.7 2,-0.0 2,-0.6 -0.974 11.5-163.3-123.8 131.9 4.4 9.8 1.8 18 18 A T E +d 93 0B 88 -2,-0.4 2,-0.2 74,-0.2 76,-0.2 -0.907 21.6 163.7-117.6 105.0 8.0 10.9 1.8 19 19 A A E -d 94 0B 13 74,-1.2 76,-2.1 -2,-0.6 2,-0.1 -0.690 31.5-113.4-114.8 168.9 10.5 8.5 3.2 20 20 A Y E > -d 95 0B 101 74,-0.3 3,-0.9 -2,-0.2 2,-0.3 -0.369 47.8 -70.9 -94.4 176.2 14.3 8.1 3.0 21 21 A C T 3 S+ 0 0 48 74,-1.8 51,-0.3 1,-0.3 76,-0.1 -0.508 119.9 4.1 -70.8 128.9 16.4 5.4 1.4 22 22 A G T 3 S+ 0 0 50 49,-1.6 48,-0.4 -2,-0.3 -1,-0.3 0.262 102.7 131.5 81.6 -14.1 16.3 2.1 3.2 23 23 A E E < - B 0 71A 105 -3,-0.9 48,-2.8 48,-0.6 2,-0.6 -0.108 65.4-101.8 -65.5 168.3 13.7 3.6 5.6 24 24 A R E - 0 0 118 46,-0.2 2,-0.4 45,-0.1 45,-0.2 -0.854 32.9-154.9-100.0 119.8 10.5 1.8 6.4 25 25 A V E - B 0 68A 2 43,-1.4 43,-2.6 -2,-0.6 2,-0.4 -0.750 8.1-169.4 -95.4 138.9 7.3 3.1 4.6 26 26 A E E - B 0 67A 91 -2,-0.4 2,-0.7 41,-0.2 41,-0.2 -0.965 4.8-167.2-132.6 117.2 3.9 2.6 6.1 27 27 A L E - B 0 66A 5 39,-1.8 39,-2.7 -2,-0.4 2,-0.3 -0.892 11.3-170.7-107.4 109.7 0.7 3.3 4.2 28 28 A E E + B 0 65A 73 -2,-0.7 2,-0.3 37,-0.2 -13,-0.3 -0.699 12.5 158.5 -99.0 151.0 -2.5 3.4 6.4 29 29 A C E - B 0 64A 1 35,-1.9 35,-1.6 -2,-0.3 2,-0.3 -0.980 26.4-134.4-162.0 165.5 -6.0 3.6 5.1 30 30 A E E -AB 12 63A 78 -18,-1.0 -18,-1.3 -2,-0.3 -19,-0.3 -0.949 15.4-167.0-130.8 150.7 -9.7 2.9 6.1 31 31 A V - 0 0 4 31,-2.3 -21,-0.2 -2,-0.3 -23,-0.0 -0.642 33.9-110.6-125.0-177.2 -12.6 1.1 4.4 32 32 A S S S+ 0 0 42 -23,-0.4 2,-0.2 -2,-0.2 -22,-0.1 0.829 98.7 55.4 -84.9 -35.9 -16.3 0.8 4.7 33 33 A E S S- 0 0 84 -24,-0.1 3,-0.4 1,-0.1 -2,-0.3 -0.528 84.3-117.7 -95.7 164.5 -16.3 -2.9 5.8 34 34 A D S S+ 0 0 87 1,-0.2 28,-0.2 -2,-0.2 3,-0.1 -0.587 88.2 7.2 -99.3 163.1 -14.4 -4.5 8.6 35 35 A D S S+ 0 0 117 26,-2.8 -1,-0.2 -2,-0.2 27,-0.1 0.806 94.3 164.1 34.5 39.9 -11.6 -7.1 8.5 36 36 A A - 0 0 10 -3,-0.4 2,-0.4 25,-0.4 -1,-0.1 -0.137 41.1-124.1 -76.6 177.3 -11.8 -6.6 4.7 37 37 A N - 0 0 132 -3,-0.1 2,-0.1 27,-0.0 -1,-0.0 -0.767 29.9-174.4-129.8 87.1 -9.2 -7.7 2.2 38 38 A V - 0 0 4 -2,-0.4 2,-0.6 45,-0.1 45,-0.2 -0.347 21.9-126.5 -77.5 160.6 -7.9 -4.9 0.1 39 39 A K E -E 82 0C 103 43,-2.2 43,-2.0 -2,-0.1 2,-0.5 -0.930 17.4-154.3-114.7 112.4 -5.5 -5.3 -2.9 40 40 A W E -E 81 0C 4 -2,-0.6 7,-2.1 41,-0.2 2,-0.4 -0.721 13.2-171.9 -87.7 127.6 -2.4 -3.2 -2.9 41 41 A F E -EF 80 46C 27 39,-2.8 39,-2.6 -2,-0.5 2,-0.8 -0.968 17.5-144.1-123.5 135.2 -0.8 -2.5 -6.3 42 42 A K E > S-EF 79 45C 30 3,-1.9 3,-1.6 -2,-0.4 37,-0.2 -0.830 84.8 -28.0-100.6 102.4 2.5 -0.9 -7.0 43 43 A N T 3 S- 0 0 100 35,-2.7 -1,-0.2 -2,-0.8 36,-0.1 0.817 129.7 -45.3 63.8 31.0 2.3 1.2 -10.1 44 44 A G T 3 S+ 0 0 65 1,-0.4 -1,-0.3 34,-0.4 2,-0.1 0.054 116.0 117.8 101.2 -25.1 -0.5 -1.0 -11.4 45 45 A E E < -F 42 0C 102 -3,-1.6 -3,-1.9 1,-0.1 2,-0.5 -0.464 68.7-115.2 -77.1 148.0 1.3 -4.2 -10.5 46 46 A E E -F 41 0C 107 -5,-0.2 2,-0.3 -2,-0.1 -5,-0.2 -0.715 26.2-135.5 -87.4 128.4 -0.2 -6.6 -8.0 47 47 A I - 0 0 17 -7,-2.1 -7,-0.1 -2,-0.5 -1,-0.0 -0.598 15.0-166.5 -84.0 142.2 1.8 -7.1 -4.8 48 48 A I - 0 0 121 -2,-0.3 7,-0.1 2,-0.0 -2,-0.0 -0.778 11.8-179.1-133.1 89.6 2.2 -10.6 -3.4 49 49 A P + 0 0 45 0, 0.0 7,-0.1 0, 0.0 3,-0.1 0.191 15.9 132.1 -69.8-165.2 3.5 -10.7 0.2 50 50 A G S > S- 0 0 42 5,-0.5 3,-1.0 1,-0.1 -2,-0.0 -0.950 70.6 -45.3 149.1-168.4 4.3 -13.8 2.3 51 51 A P T 3 S+ 0 0 140 0, 0.0 -1,-0.1 0, 0.0 0, 0.0 0.693 137.1 35.2 -69.8 -19.2 6.9 -15.4 4.6 52 52 A K T 3 S+ 0 0 203 -3,-0.1 2,-0.3 0, 0.0 0, 0.0 -0.332 99.2 103.6-131.4 51.5 9.6 -14.4 2.1 53 53 A S < - 0 0 48 -3,-1.0 -3,-0.1 1,-0.1 3,-0.0 -0.806 64.2-135.3-128.2 169.4 8.5 -11.0 0.8 54 54 A R S S+ 0 0 132 -2,-0.3 15,-1.4 14,-0.0 2,-0.3 0.839 86.9 68.2 -91.8 -40.8 9.3 -7.4 1.3 55 55 A Y E -C 68 0A 32 13,-0.2 -5,-0.5 -7,-0.1 2,-0.5 -0.622 63.6-166.1 -84.3 139.2 5.8 -6.0 1.6 56 56 A R E -C 67 0A 122 11,-1.8 11,-1.7 -2,-0.3 2,-0.5 -0.917 6.2-155.5-130.8 106.6 3.6 -6.9 4.6 57 57 A I E -C 66 0A 57 -2,-0.5 2,-0.4 9,-0.2 9,-0.2 -0.699 14.6-170.7 -84.1 121.6 -0.1 -6.2 4.5 58 58 A R E -C 65 0A 125 7,-1.7 7,-2.1 -2,-0.5 2,-0.5 -0.918 10.8-151.2-116.0 139.3 -1.6 -5.8 8.0 59 59 A V E +C 64 0A 50 -2,-0.4 2,-0.5 5,-0.2 5,-0.2 -0.936 15.2 176.1-113.2 122.3 -5.3 -5.7 8.8 60 60 A E E > -C 63 0A 112 3,-2.8 3,-2.1 -2,-0.5 2,-0.7 -0.885 59.3 -67.7-128.4 101.0 -6.5 -3.7 11.8 61 61 A G T 3 S- 0 0 38 -2,-0.5 -26,-2.8 1,-0.3 -25,-0.4 -0.419 117.6 -15.1 61.3-106.0 -10.3 -3.5 12.4 62 62 A K T 3 S+ 0 0 101 -2,-0.7 -31,-2.3 -28,-0.2 2,-0.4 0.026 125.9 77.8-118.0 24.3 -11.5 -1.3 9.5 63 63 A K E < -BC 30 60A 71 -3,-2.1 -3,-2.8 -33,-0.2 2,-0.3 -0.997 62.0-150.6-137.5 139.8 -8.1 0.1 8.5 64 64 A H E -BC 29 59A 1 -35,-1.6 -35,-1.9 -2,-0.4 2,-0.4 -0.743 2.6-158.9-108.0 156.6 -5.2 -1.5 6.5 65 65 A I E -BC 28 58A 12 -7,-2.1 -7,-1.7 -2,-0.3 2,-0.9 -0.897 1.0-163.8-140.1 108.0 -1.4 -0.8 6.8 66 66 A L E -BC 27 57A 0 -39,-2.7 -39,-1.8 -2,-0.4 2,-0.3 -0.785 16.0-169.6 -94.6 101.1 1.0 -1.6 3.9 67 67 A I E -BC 26 56A 9 -11,-1.7 -11,-1.8 -2,-0.9 2,-0.5 -0.647 8.5-153.9 -91.3 147.1 4.5 -1.6 5.3 68 68 A I E -BC 25 55A 0 -43,-2.6 -43,-1.4 -2,-0.3 2,-0.8 -0.954 9.3-143.0-126.1 113.7 7.6 -1.7 3.1 69 69 A E E S+ 0 0 88 -15,-1.4 -45,-0.1 -2,-0.5 -46,-0.1 -0.629 83.4 2.8 -76.9 108.8 10.9 -3.1 4.4 70 70 A G E S- 0 0 23 -2,-0.8 2,-1.1 -48,-0.4 -47,-0.2 0.935 73.6-146.2 79.9 86.9 13.7 -1.0 3.0 71 71 A A E +B 23 0A 0 -48,-2.8 -49,-1.6 -51,-0.2 -48,-0.6 -0.718 28.2 176.3 -88.4 96.8 12.3 1.9 0.9 72 72 A T >> - 0 0 70 -2,-1.1 4,-1.3 -51,-0.3 3,-0.6 -0.374 52.4 -86.6 -93.4 174.8 14.9 2.3 -1.9 73 73 A K T 34 S+ 0 0 130 1,-0.3 21,-0.2 2,-0.2 -1,-0.1 0.666 132.6 52.3 -54.6 -14.5 14.9 4.7 -4.9 74 74 A A T 34 S+ 0 0 70 1,-0.1 -1,-0.3 3,-0.0 -3,-0.1 0.834 96.8 61.8 -90.0 -38.9 12.9 1.9 -6.5 75 75 A D T <4 S+ 0 0 4 -3,-0.6 2,-1.2 -5,-0.1 -2,-0.2 0.815 79.3 99.8 -56.9 -31.1 10.2 1.5 -3.8 76 76 A A < + 0 0 33 -4,-1.3 2,-0.3 15,-0.1 17,-0.1 -0.419 58.1 122.5 -61.9 94.5 9.2 5.1 -4.5 77 77 A A S S- 0 0 9 -2,-1.2 2,-1.1 16,-0.1 15,-0.9 -0.926 72.9 -76.9-148.7 171.6 6.2 4.4 -6.8 78 78 A E E - G 0 91C 91 -2,-0.3 -35,-2.7 13,-0.2 2,-0.7 -0.631 46.5-161.1 -78.3 100.4 2.5 5.1 -7.1 79 79 A Y E +EG 42 90C 0 -2,-1.1 11,-1.0 11,-0.6 2,-0.4 -0.745 13.1 178.9 -87.6 115.9 0.8 2.7 -4.6 80 80 A S E -EG 41 89C 23 -39,-2.6 -39,-2.8 -2,-0.7 9,-0.3 -0.958 14.4-148.5-121.4 135.5 -2.8 2.2 -5.4 81 81 A V E -EG 40 88C 2 7,-0.9 7,-2.0 -2,-0.4 2,-0.4 -0.669 5.8-157.9-100.1 155.8 -5.3 -0.1 -3.5 82 82 A M E +EG 39 87C 104 -43,-2.0 -43,-2.2 -2,-0.2 5,-0.2 -0.978 12.6 176.9-138.3 123.3 -8.3 -2.0 -4.9 83 83 A T E > - G 0 86C 19 3,-3.2 3,-0.5 -2,-0.4 -45,-0.1 -0.473 51.6 -84.2-112.6-174.9 -11.3 -3.2 -2.9 84 84 A T T 3 S+ 0 0 107 1,-0.2 -77,-0.1 -2,-0.2 -75,-0.1 0.735 127.5 2.9 -63.2 -21.8 -14.5 -5.0 -3.8 85 85 A G T 3 S+ 0 0 30 -77,-0.4 2,-0.3 -78,-0.1 -1,-0.2 -0.040 124.2 56.6-158.4 42.8 -16.0 -1.5 -4.5 86 86 A G E < -G 83 0C 20 -3,-0.5 -3,-3.2 -77,-0.2 2,-0.3 -0.984 48.4-163.1-168.7 161.9 -13.3 1.1 -4.1 87 87 A Q E -G 82 0C 144 -2,-0.3 2,-0.3 -5,-0.2 -5,-0.2 -0.992 6.4-177.1-154.7 147.7 -9.8 2.3 -5.2 88 88 A S E -G 81 0C 5 -7,-2.0 -7,-0.9 -2,-0.3 2,-0.3 -0.948 5.8-161.0-143.4 162.3 -7.1 4.6 -4.0 89 89 A S E +G 80 0C 58 -2,-0.3 2,-0.3 -9,-0.3 -9,-0.2 -0.996 8.6 177.1-147.0 148.8 -3.7 6.0 -5.1 90 90 A A E -G 79 0C 10 -11,-1.0 -11,-0.6 -2,-0.3 2,-0.5 -0.936 25.9-121.5-146.1 167.4 -0.7 7.7 -3.5 91 91 A K E -G 78 0C 133 -2,-0.3 -74,-1.8 -13,-0.2 2,-0.7 -0.960 18.7-166.9-119.6 120.0 2.8 8.9 -4.3 92 92 A L E -d 17 0B 8 -15,-0.9 2,-0.5 -2,-0.5 -74,-0.2 -0.900 6.4-170.3-109.4 109.4 5.8 7.6 -2.4 93 93 A S E -d 18 0B 55 -76,-1.7 -74,-1.2 -2,-0.7 2,-0.5 -0.864 8.2-152.7-102.6 125.9 9.0 9.7 -2.9 94 94 A V E -d 19 0B 13 -2,-0.5 2,-0.4 -76,-0.2 -74,-0.3 -0.835 8.1-162.6-100.4 130.6 12.3 8.3 -1.6 95 95 A D E -d 20 0B 71 -76,-2.1 -74,-1.8 -2,-0.5 2,-0.9 -0.925 20.0-126.3-115.2 136.0 15.1 10.6 -0.6 96 96 A L - 0 0 105 -2,-0.4 -76,-0.1 -76,-0.2 -24,-0.0 -0.692 37.7-110.6 -82.7 106.6 18.7 9.7 -0.2 97 97 A K - 0 0 111 -2,-0.9 2,-0.2 -76,-0.1 4,-0.1 -0.031 36.1-133.6 -36.6 122.3 19.8 10.8 3.2 98 98 A S + 0 0 120 2,-0.1 -1,-0.1 -3,-0.0 -3,-0.0 -0.603 62.9 29.8 -86.5 145.2 22.3 13.7 2.7 99 99 A G S S- 0 0 51 -2,-0.2 2,-0.4 2,-0.1 0, 0.0 -0.004 102.3 -48.7 92.9 159.2 25.6 13.8 4.6 100 100 A P - 0 0 125 0, 0.0 2,-1.6 0, 0.0 -2,-0.1 -0.518 51.3-133.1 -69.7 120.4 27.9 11.1 5.9 101 101 A S + 0 0 119 -2,-0.4 2,-0.3 -4,-0.1 -2,-0.1 -0.573 54.0 137.4 -77.3 88.6 26.0 8.5 8.0 102 102 A S 0 0 114 -2,-1.6 0, 0.0 1,-0.0 0, 0.0 -0.983 360.0 360.0-136.5 147.0 28.2 8.3 11.1 103 103 A G 0 0 127 -2,-0.3 -2,-0.1 0, 0.0 -1,-0.0 0.237 360.0 360.0 69.8 360.0 27.6 8.2 14.8