==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=6-JAN-2010 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER IMMUNE SYSTEM 22-JUN-07 2Z4Q . COMPND 2 MOLECULE: ANTI EGFR ANTIBODY FAB, LIGHT CHAIN; . SOURCE 2 ORGANISM_SCIENTIFIC: MUS MUSCULUS; . AUTHOR T.NAKANISHI,K.TSUMOTO,R.ASANO,H.KONDO,I.KUMAGAI . 437 2 5 4 1 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 19924.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 288 65.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 11 2.5 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 175 40.0 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 5 1.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 2 0.5 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 49 11.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 31 7.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 13 3.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 4 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 5 3 8 2 4 8 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 5 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 A D 0 0 150 0, 0.0 2,-0.5 0, 0.0 98,-0.0 0.000 360.0 360.0 360.0 138.6 -54.9 2.4 -15.9 2 2 A I - 0 0 9 98,-0.4 2,-0.5 96,-0.1 93,-0.1 -0.585 360.0-144.1 -73.8 121.9 -53.3 4.5 -13.2 3 3 A L - 0 0 94 -2,-0.5 23,-1.6 99,-0.0 2,-0.7 -0.773 7.7-155.3 -89.8 124.2 -49.7 5.0 -13.9 4 4 A M E -A 25 0A 8 -2,-0.5 2,-0.7 99,-0.4 101,-0.6 -0.891 7.4-163.8-104.1 107.0 -47.5 5.1 -10.8 5 5 A T E -A 24 0A 42 19,-3.0 19,-3.0 -2,-0.7 2,-0.4 -0.828 9.1-172.1 -96.1 117.2 -44.3 7.0 -11.4 6 6 A Q E -A 23 0A 18 -2,-0.7 17,-0.2 17,-0.2 99,-0.1 -0.896 11.1-132.5-113.9 135.5 -41.6 6.4 -8.8 7 7 A T E +A 22 0A 79 15,-2.3 15,-2.7 -2,-0.4 2,-0.1 -0.983 67.0 31.2-143.4 150.1 -38.3 8.2 -8.3 8 8 A P - 0 0 50 0, 0.0 3,-0.1 0, 0.0 -1,-0.1 0.628 56.7-154.6 -84.1 172.8 -35.4 7.9 -7.9 9 9 A L S S+ 0 0 130 1,-0.2 99,-2.5 98,-0.2 2,-0.4 0.874 87.4 25.3 -71.8 -39.7 -34.5 4.6 -9.7 10 10 A S E -d 108 0B 78 97,-0.2 -1,-0.2 80,-0.0 99,-0.2 -0.980 67.5-170.0-127.9 139.1 -31.5 4.2 -7.4 11 11 A L E -d 109 0B 26 97,-2.7 99,-2.1 -2,-0.4 2,-0.6 -0.835 2.0-168.9-135.6 97.5 -31.0 5.6 -3.9 12 12 A P E +d 110 0B 64 0, 0.0 2,-0.4 0, 0.0 99,-0.2 -0.745 20.2 175.5 -84.7 121.4 -27.6 5.4 -2.2 13 13 A V E -d 111 0B 8 97,-3.0 99,-3.0 -2,-0.6 2,-0.1 -0.989 29.4-121.1-136.7 137.3 -27.9 6.3 1.4 14 14 A S E > -d 112 0B 40 -2,-0.4 3,-2.6 97,-0.2 69,-0.3 -0.412 42.1 -96.9 -70.3 143.3 -25.6 6.4 4.4 15 15 A L T 3 S+ 0 0 83 97,-1.5 69,-0.2 1,-0.3 -1,-0.1 -0.374 115.5 23.1 -56.8 133.8 -26.5 4.3 7.4 16 16 A G T 3 S+ 0 0 16 67,-2.7 66,-0.5 1,-0.3 -1,-0.3 0.189 102.7 109.3 91.9 -18.6 -28.2 6.6 9.9 17 17 A D S < S- 0 0 86 -3,-2.6 66,-2.9 65,-0.2 2,-0.4 -0.335 74.1 -99.8 -85.6 173.2 -29.2 9.1 7.2 18 18 A Q - 0 0 115 64,-0.2 2,-0.4 63,-0.2 63,-0.2 -0.767 28.3-154.2 -97.8 137.7 -32.7 9.7 5.9 19 19 A A E - B 0 80A 0 61,-2.8 61,-2.7 -2,-0.4 2,-0.4 -0.897 6.9-166.3-109.9 138.5 -34.1 8.1 2.7 20 20 A S E - B 0 79A 53 -2,-0.4 2,-0.5 59,-0.2 59,-0.2 -0.966 3.9-174.3-130.3 117.7 -36.9 9.8 0.7 21 21 A I E - B 0 78A 0 57,-3.0 57,-2.8 -2,-0.4 2,-0.3 -0.940 10.4-154.0-114.7 128.1 -38.7 7.9 -2.1 22 22 A S E +AB 7 77A 47 -15,-2.7 -15,-2.3 -2,-0.5 2,-0.3 -0.731 13.0 179.1-105.4 150.2 -41.3 9.7 -4.3 23 23 A a E -AB 6 76A 3 53,-2.3 53,-2.7 -2,-0.3 2,-0.4 -0.974 5.5-168.6-148.4 127.1 -44.3 8.3 -6.2 24 24 A R E -AB 5 75A 129 -19,-3.0 -19,-3.0 -2,-0.3 2,-0.3 -0.966 13.2-143.1-121.7 135.7 -46.8 10.2 -8.3 25 25 A S E -A 4 0A 3 49,-1.7 -21,-0.2 -2,-0.4 4,-0.1 -0.701 17.3-131.3 -93.4 147.8 -50.1 8.8 -9.6 26 26 A S S S+ 0 0 59 -23,-1.6 2,-0.3 -2,-0.3 -1,-0.1 0.657 89.3 7.1 -70.5 -14.4 -51.3 9.8 -13.1 27 27 A Q S S- 0 0 84 -24,-0.2 -2,-0.1 46,-0.1 -1,-0.1 -0.960 99.9 -59.2-157.2 172.3 -54.7 10.4 -11.5 28 28 A N - 0 0 88 -2,-0.3 46,-0.1 1,-0.1 -2,-0.1 -0.293 39.4-170.8 -55.9 137.8 -56.7 10.7 -8.2 29 29 A I + 0 0 0 44,-0.6 2,-0.7 2,-0.1 8,-0.6 -0.046 37.8 128.3-125.1 32.4 -56.6 7.4 -6.3 30 30 A V B -Q 36 0C 55 6,-0.2 6,-0.2 7,-0.1 5,-0.1 -0.810 56.8-132.7 -89.7 117.1 -59.2 8.0 -3.6 31 31 A H > - 0 0 71 4,-2.7 3,-2.2 -2,-0.7 67,-0.1 -0.204 26.1-103.8 -62.3 161.1 -61.6 5.1 -3.6 32 32 A N T 3 S+ 0 0 168 1,-0.3 -1,-0.1 65,-0.2 -2,-0.0 0.795 121.9 64.2 -58.6 -27.3 -65.3 6.0 -3.5 33 33 A N T 3 S- 0 0 85 2,-0.2 -1,-0.3 1,-0.1 3,-0.1 0.361 122.0-107.7 -78.2 8.2 -65.3 4.9 0.2 34 34 A G S < S+ 0 0 52 -3,-2.2 2,-0.4 1,-0.2 -2,-0.1 0.385 80.9 125.7 83.9 -4.9 -63.0 7.8 0.8 35 35 A I - 0 0 49 -5,-0.1 -4,-2.7 62,-0.0 2,-0.8 -0.739 52.9-145.3 -94.4 134.8 -59.9 5.6 1.4 36 36 A T B -Q 30 0C 28 -2,-0.4 2,-1.9 -6,-0.2 -6,-0.2 -0.848 8.5-156.8 -98.0 106.3 -56.7 6.1 -0.6 37 37 A Y + 0 0 28 -2,-0.8 59,-2.0 -8,-0.6 2,-0.6 -0.357 36.7 147.2 -82.0 59.2 -55.2 2.6 -1.1 38 38 A L E +E 95 0B 0 -2,-1.9 18,-2.9 57,-0.2 2,-0.3 -0.861 22.7 176.7 -96.2 125.0 -51.6 3.8 -1.6 39 39 A E E -E 94 0B 0 55,-3.1 55,-1.9 -2,-0.6 2,-0.4 -0.889 24.1-143.7-129.6 158.4 -49.1 1.2 -0.3 40 40 A W E -EF 93 53B 0 13,-2.1 12,-2.7 -2,-0.3 13,-1.4 -0.986 17.5-170.4-123.7 131.5 -45.4 0.7 -0.1 41 41 A Y E -EF 92 51B 0 51,-2.7 51,-2.0 -2,-0.4 2,-0.4 -0.837 10.6-152.2-118.7 155.6 -43.7 -2.7 -0.4 42 42 A L E -EF 91 50B 21 8,-2.3 8,-2.0 -2,-0.3 2,-0.4 -0.997 14.2-172.9-129.7 126.3 -40.1 -3.7 0.2 43 43 A Q E -E 90 0B 12 47,-2.4 47,-2.4 -2,-0.4 5,-0.1 -0.978 12.4-154.3-125.4 129.2 -38.6 -6.7 -1.7 44 44 A R > - 0 0 75 -2,-0.4 3,-2.2 4,-0.4 45,-0.1 -0.736 46.9 -84.3 -93.2 147.2 -35.3 -8.4 -1.2 45 45 A P T 3 S+ 0 0 66 0, 0.0 -1,-0.1 0, 0.0 3,-0.1 -0.223 115.8 14.9 -52.7 134.9 -33.9 -10.2 -4.2 46 46 A G T 3 S+ 0 0 85 1,-0.3 2,-0.3 -3,-0.1 -3,-0.0 0.489 112.4 93.5 80.1 1.9 -35.2 -13.7 -4.5 47 47 A Q S < S- 0 0 80 -3,-2.2 -1,-0.3 3,-0.0 3,-0.1 -0.849 80.1 -98.8-125.7 163.8 -38.0 -13.1 -2.0 48 48 A S - 0 0 29 -2,-0.3 -4,-0.4 1,-0.1 -6,-0.1 -0.447 51.2 -91.6 -77.2 150.7 -41.7 -12.0 -2.2 49 49 A P - 0 0 3 0, 0.0 2,-0.4 0, 0.0 -6,-0.2 -0.224 37.4-143.0 -60.8 151.7 -42.5 -8.3 -1.5 50 50 A K E -F 42 0B 127 -8,-2.0 -8,-2.3 -3,-0.1 2,-0.3 -0.968 14.3-121.4-120.9 136.7 -43.4 -7.3 2.0 51 51 A L E +F 41 0B 9 -2,-0.4 -10,-0.2 275,-0.3 3,-0.1 -0.599 38.6 160.3 -77.2 129.9 -46.0 -4.8 2.9 52 52 A L E + 0 0 16 -12,-2.7 8,-3.1 1,-0.4 2,-0.4 0.758 63.0 21.6-113.0 -53.7 -44.7 -1.8 4.9 53 53 A I E -FG 40 59B 0 -13,-1.4 -13,-2.1 6,-0.2 -1,-0.4 -0.959 63.6-179.8-122.9 137.9 -47.1 1.1 4.6 54 54 A Y E > + G 0 58B 45 4,-3.0 4,-2.5 -2,-0.4 3,-0.3 -0.943 64.3 24.2-134.9 157.2 -50.9 0.9 3.8 55 55 A K T 4 S- 0 0 67 -2,-0.3 2,-2.4 1,-0.3 -1,-0.2 0.918 124.6 -72.2 53.1 46.9 -53.6 3.5 3.4 56 56 A V T 4 S+ 0 0 19 -18,-2.9 -1,-0.3 1,-0.2 21,-0.1 -0.256 128.0 19.6 70.5 -51.7 -50.9 6.1 2.7 57 57 A S T 4 S+ 0 0 63 -2,-2.4 2,-0.5 -3,-0.3 12,-0.3 0.309 88.3 112.6-134.6 10.1 -49.5 6.4 6.2 58 58 A D E < -G 54 0B 60 -4,-2.5 -4,-3.0 10,-0.1 2,-0.4 -0.763 59.0-134.8 -90.9 130.5 -50.4 3.4 8.3 59 59 A R E -G 53 0B 107 -2,-0.5 -6,-0.2 -6,-0.2 3,-0.1 -0.694 19.8-124.2 -85.1 129.1 -47.6 1.1 9.3 60 60 A F > - 0 0 40 -8,-3.1 3,-2.0 -2,-0.4 -1,-0.1 -0.285 49.0 -71.6 -66.4 159.5 -48.2 -2.6 8.8 61 61 A S T 3 S+ 0 0 109 1,-0.3 -1,-0.2 3,-0.0 0, 0.0 -0.286 120.8 15.9 -56.5 125.2 -47.8 -4.8 11.9 62 62 A G T 3 S+ 0 0 78 1,-0.3 -1,-0.3 -3,-0.1 -2,-0.1 0.347 88.4 133.3 95.1 -6.5 -44.1 -5.1 12.9 63 63 A V < - 0 0 22 -3,-2.0 -1,-0.3 -11,-0.1 -4,-0.1 -0.690 61.3-113.3 -84.3 125.8 -42.8 -2.2 10.8 64 64 A P > - 0 0 58 0, 0.0 3,-1.9 0, 0.0 -1,-0.1 -0.192 20.4-122.1 -54.1 141.8 -40.4 0.1 12.6 65 65 A D T 3 S+ 0 0 130 1,-0.3 16,-0.1 -6,-0.1 -2,-0.0 0.502 104.0 80.2 -68.9 -1.9 -41.7 3.6 13.2 66 66 A R T 3 S+ 0 0 32 14,-0.1 15,-2.4 16,-0.1 2,-0.5 0.597 78.2 84.6 -79.1 -7.2 -38.7 5.1 11.3 67 67 A F E < +C 80 0A 7 -3,-1.9 2,-0.4 13,-0.2 13,-0.2 -0.839 64.4 175.7 -93.6 125.9 -40.7 4.2 8.2 68 68 A S E -C 79 0A 53 11,-2.7 11,-3.5 -2,-0.5 2,-0.4 -0.993 16.6-164.1-140.0 131.7 -43.1 6.9 7.4 69 69 A G E +C 78 0A 6 -2,-0.4 2,-0.3 -12,-0.3 9,-0.2 -0.875 18.8 155.9-114.1 146.4 -45.6 7.5 4.5 70 70 A S E +C 77 0A 52 7,-2.1 7,-2.6 -2,-0.4 2,-0.2 -0.926 13.9 91.6-154.2 176.7 -47.3 10.7 3.6 71 71 A G E -C 76 0A 37 -2,-0.3 2,-0.3 5,-0.3 5,-0.2 -0.517 32.7-152.4 120.6 171.8 -48.9 12.5 0.7 72 72 A S E > +C 75 0A 91 3,-1.7 3,-1.8 -2,-0.2 2,-0.8 -0.954 62.0 28.7-172.0 168.2 -52.2 13.1 -1.1 73 73 A G T 3 S- 0 0 44 -2,-0.3 -44,-0.6 1,-0.2 -46,-0.1 -0.599 129.7 -30.1 73.9-109.8 -53.6 14.0 -4.5 74 74 A T T 3 S+ 0 0 55 -2,-0.8 -49,-1.7 -46,-0.1 2,-0.5 0.288 122.8 81.0-124.7 6.0 -51.1 12.6 -6.9 75 75 A D E < +BC 24 72A 100 -3,-1.8 -3,-1.7 -51,-0.2 2,-0.3 -0.963 50.1 170.5-122.2 127.4 -48.0 12.9 -4.9 76 76 A F E -BC 23 71A 3 -53,-2.7 -53,-2.3 -2,-0.5 2,-0.4 -0.957 11.8-160.0-133.6 150.6 -46.9 10.4 -2.2 77 77 A T E -BC 22 70A 31 -7,-2.6 -7,-2.1 -2,-0.3 2,-0.5 -0.994 9.7-159.1-137.9 137.2 -43.6 10.0 -0.2 78 78 A L E -BC 21 69A 0 -57,-2.8 -57,-3.0 -2,-0.4 2,-0.4 -0.964 18.4-169.8-112.0 124.3 -42.0 7.2 1.8 79 79 A K E -BC 20 68A 87 -11,-3.5 -11,-2.7 -2,-0.5 2,-0.6 -0.950 11.3-166.9-118.9 136.0 -39.4 8.4 4.3 80 80 A I E -BC 19 67A 0 -61,-2.7 -61,-2.8 -2,-0.4 3,-0.4 -0.959 11.6-160.7-121.1 109.5 -37.0 6.2 6.2 81 81 A S S S+ 0 0 45 -15,-2.4 -63,-0.2 -2,-0.6 -64,-0.1 -0.616 73.4 12.1 -87.4 151.1 -35.3 8.0 9.0 82 82 A R S S- 0 0 164 -66,-0.5 -1,-0.2 -2,-0.2 -64,-0.2 0.940 89.8-140.8 49.7 59.3 -32.1 6.5 10.4 83 83 A V - 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