==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=6-JAN-2010 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER FLUORESCENT PROTEIN 09-AUG-07 2Z6Z . COMPND 2 MOLECULE: FLUORESCENT PROTEIN DRONPA; . SOURCE 2 ORGANISM_SCIENTIFIC: ECHINOPHYLLIA SP. SC22; . AUTHOR A.KIKUCHI,J.JEYAKANTHAN,J.TAKA,Y.SHIRO,H.MIZUNO,A.MIYAWAKI . 1292 12 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 51107.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 958 74.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 78 6.0 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 612 47.4 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 12 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 12 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 18 1.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 108 8.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 91 7.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 48 3.7 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 12 0.9 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 9 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 6 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 0 0 6 0 12 0 6 6 12 12 12 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 0 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 2 A S 0 0 120 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 132.7 -15.8 72.4 -20.1 2 3 A V + 0 0 51 75,-0.1 2,-0.8 78,-0.1 75,-0.0 0.632 360.0 86.6 -82.7 -15.7 -14.7 74.5 -17.1 3 4 A I - 0 0 8 77,-0.1 106,-0.1 74,-0.1 4,-0.1 -0.784 64.7-172.5 -90.7 108.7 -11.1 73.6 -17.7 4 5 A K - 0 0 105 -2,-0.8 29,-0.2 2,-0.1 -2,-0.1 -0.637 37.7-108.7-100.0 159.0 -9.7 76.1 -20.3 5 6 A P S S+ 0 0 86 0, 0.0 2,-0.4 0, 0.0 29,-0.2 0.799 110.7 37.1 -52.8 -34.0 -6.2 76.0 -22.0 6 7 A D S S+ 0 0 99 25,-0.2 2,-0.3 26,-0.1 25,-0.2 -0.988 76.6 168.7-126.1 124.0 -5.2 79.0 -19.9 7 8 A M E -A 30 0A 2 23,-2.4 23,-2.5 -2,-0.4 103,-0.2 -0.928 24.3-126.4-135.3 159.4 -6.2 79.5 -16.3 8 9 A K E -Ab 29 110A 100 101,-3.0 103,-2.7 -2,-0.3 2,-0.4 -0.559 14.3-148.2-101.4 166.5 -5.4 81.6 -13.3 9 10 A I E +Ab 28 111A 5 19,-2.6 19,-2.5 101,-0.2 2,-0.4 -0.999 13.7 176.7-138.0 137.3 -4.4 80.9 -9.8 10 11 A K E +Ab 27 112A 128 101,-2.3 103,-2.7 -2,-0.4 2,-0.3 -0.998 16.8 162.7-138.6 131.9 -5.0 82.6 -6.5 11 12 A L E -Ab 26 113A 10 15,-2.1 15,-2.4 -2,-0.4 2,-0.3 -0.987 25.8-162.2-150.6 160.2 -3.9 81.3 -3.1 12 13 A R E -Ab 25 114A 71 101,-1.4 103,-2.5 -2,-0.3 2,-0.5 -0.972 11.9-163.7-144.7 127.2 -3.2 82.1 0.5 13 14 A M E -Ab 24 115A 0 11,-3.5 11,-2.5 -2,-0.3 2,-0.4 -0.955 5.8-176.8-115.3 130.0 -1.2 79.9 2.9 14 15 A E E +Ab 23 116A 92 101,-2.1 103,-1.8 -2,-0.5 2,-0.3 -0.957 29.8 120.0-119.6 140.1 -1.3 80.3 6.6 15 16 A G E -Ab 22 117A 2 7,-2.3 7,-2.0 -2,-0.4 2,-0.3 -0.950 44.1-120.2-172.2-165.9 1.1 78.1 8.7 16 17 A A E -Ab 21 118A 10 101,-2.1 103,-2.6 -2,-0.3 2,-0.4 -0.982 5.5-154.8-159.2 149.0 3.9 77.6 11.1 17 18 A V E > S-Ab 20 119A 3 3,-1.7 3,-1.7 -2,-0.3 103,-0.2 -0.990 86.0 -14.4-130.8 121.6 7.3 76.0 11.3 18 19 A N T 3 S- 0 0 10 101,-2.8 102,-0.2 -2,-0.4 -1,-0.1 0.842 132.5 -51.5 55.5 33.9 8.7 74.9 14.7 19 20 A G T 3 S+ 0 0 34 100,-0.6 -1,-0.3 1,-0.2 101,-0.1 0.530 111.1 121.4 83.7 6.0 5.9 77.0 16.3 20 21 A H E < -A 17 0A 124 -3,-1.7 -3,-1.7 26,-0.0 -1,-0.2 -0.904 54.9-139.9-109.0 111.1 6.6 80.2 14.4 21 22 A P E +A 16 0A 88 0, 0.0 2,-0.3 0, 0.0 -5,-0.3 -0.210 28.3 160.4 -67.2 153.7 3.6 81.5 12.3 22 23 A F E -A 15 0A 5 -7,-2.0 -7,-2.3 27,-0.0 2,-0.3 -0.963 21.0-150.4-163.9 164.7 3.7 83.0 8.9 23 24 A A E -AC 14 45A 1 22,-2.1 21,-2.9 -2,-0.3 22,-2.0 -0.993 10.2-175.8-147.6 144.7 1.5 83.7 5.9 24 25 A I E -AC 13 43A 0 -11,-2.5 -11,-3.5 -2,-0.3 2,-0.3 -0.994 6.2-160.3-142.2 144.8 2.0 83.9 2.2 25 26 A E E -AC 12 42A 78 17,-2.6 17,-3.1 -2,-0.3 2,-0.3 -0.891 10.9-169.1-125.0 157.0 -0.2 84.8 -0.8 26 27 A G E -AC 11 41A 5 -15,-2.4 -15,-2.1 -2,-0.3 2,-0.4 -0.978 17.3-164.6-147.7 160.8 0.2 84.1 -4.5 27 28 A V E +AC 10 40A 86 13,-1.7 13,-2.8 -2,-0.3 2,-0.3 -0.993 30.4 143.3-142.0 132.3 -1.1 84.8 -7.9 28 29 A G E -AC 9 39A 11 -19,-2.5 -19,-2.6 -2,-0.4 2,-0.3 -0.881 32.2-137.0-155.1-171.9 -0.4 82.7 -11.0 29 30 A L E +AC 8 38A 78 9,-2.2 9,-2.0 -21,-0.3 2,-0.3 -0.983 21.2 171.3-153.9 154.5 -1.4 81.2 -14.3 30 31 A G E -AC 7 37A 6 -23,-2.5 -23,-2.4 -2,-0.3 7,-0.3 -0.982 37.1-113.4-161.0 171.2 -1.0 77.8 -16.0 31 32 A K E >>> - C 0 36A 91 5,-3.1 3,-1.5 -2,-0.3 5,-1.5 -0.863 24.5-167.2-115.0 92.9 -1.8 75.5 -18.9 32 33 A P G >45S+ 0 0 0 0, 0.0 34,-0.6 0, 0.0 3,-0.6 0.863 88.0 47.4 -45.2 -47.9 -3.7 72.6 -17.4 33 34 A F G 345S+ 0 0 98 1,-0.2 32,-0.1 -29,-0.2 -27,-0.1 0.653 113.9 46.8 -75.0 -14.2 -3.4 70.4 -20.5 34 35 A E G <45S- 0 0 100 -3,-1.5 33,-0.3 -29,-0.2 -1,-0.2 0.502 106.8-125.2-101.3 -7.9 0.3 71.1 -20.9 35 36 A G T <<5 + 0 0 0 -4,-1.2 175,-2.9 -3,-0.6 2,-0.4 0.885 67.2 126.7 66.0 39.8 1.0 70.4 -17.2 36 37 A K E < +CD 31 209A 108 -5,-1.5 -5,-3.1 173,-0.2 2,-0.3 -0.985 26.3 157.2-134.4 143.0 2.7 73.7 -16.7 37 38 A Q E -CD 30 208A 14 171,-1.5 171,-1.9 -2,-0.4 2,-0.3 -0.980 13.6-171.4-155.3 161.3 2.2 76.6 -14.2 38 39 A S E +CD 29 207A 26 -9,-2.0 -9,-2.2 -2,-0.3 2,-0.3 -0.977 10.6 179.0-154.7 151.0 4.1 79.5 -12.7 39 40 A M E -CD 28 206A 4 167,-2.0 167,-2.9 -2,-0.3 2,-0.5 -0.988 27.2-126.3-154.7 160.5 3.2 81.9 -9.9 40 41 A D E -CD 27 205A 84 -13,-2.8 -13,-1.7 -2,-0.3 2,-0.4 -0.962 28.5-161.4-114.1 123.1 4.5 84.9 -7.9 41 42 A L E -CD 26 204A 1 163,-3.1 163,-2.1 -2,-0.5 2,-0.4 -0.873 5.8-167.6-110.2 137.8 4.4 84.4 -4.1 42 43 A K E -CD 25 203A 95 -17,-3.1 -17,-2.6 -2,-0.4 2,-0.3 -0.982 21.2-130.6-128.9 124.2 4.6 87.1 -1.5 43 44 A V E +C 24 0A 29 159,-1.9 -19,-0.3 -2,-0.4 3,-0.1 -0.552 27.6 174.3 -70.0 125.7 5.2 86.7 2.3 44 45 A K E + 0 0 97 -21,-2.9 2,-0.3 -2,-0.3 -20,-0.2 0.727 63.2 17.9-103.9 -31.3 2.5 88.6 4.2 45 46 A E E S+C 23 0A 103 -22,-2.0 -22,-2.1 1,-0.1 -1,-0.3 -0.983 112.7 28.8-142.3 151.9 3.3 87.6 7.8 46 47 A G S S- 0 0 41 -2,-0.3 -1,-0.1 -24,-0.3 -22,-0.0 0.537 85.7-172.0 79.5 7.2 6.2 86.0 9.7 47 48 A G + 0 0 48 1,-0.1 -1,-0.3 2,-0.1 154,-0.1 -0.492 59.1 51.5 -81.5 153.9 8.9 87.4 7.4 48 49 A P S S- 0 0 86 0, 0.0 -1,-0.1 0, 0.0 153,-0.1 0.389 90.4-135.1 -78.9 130.7 11.8 87.2 7.1 49 50 A L - 0 0 6 151,-0.5 4,-0.1 1,-0.2 -2,-0.1 -0.268 10.5-156.7 -53.7 126.8 11.2 83.4 7.0 50 51 A P + 0 0 59 0, 0.0 2,-0.3 0, 0.0 -1,-0.2 0.394 69.8 67.0 -86.6 1.3 13.8 81.6 9.1 51 52 A F S S- 0 0 7 79,-0.1 2,-0.2 80,-0.1 78,-0.2 -0.828 100.3 -77.2-122.8 161.3 13.5 78.3 7.3 52 53 A A > - 0 0 4 76,-1.8 3,-1.8 -2,-0.3 4,-0.2 -0.371 33.9-145.3 -58.9 120.0 14.3 77.0 3.8 53 54 A Y T >> S+ 0 0 5 1,-0.3 3,-2.4 -2,-0.2 4,-0.8 0.793 90.5 78.8 -57.5 -29.4 11.6 78.3 1.5 54 55 A D H 3> S+ 0 0 9 1,-0.3 4,-0.5 2,-0.2 -1,-0.3 0.729 78.6 67.4 -54.2 -25.6 11.9 75.0 -0.5 55 56 A I H <4 S+ 0 0 0 -3,-1.8 -1,-0.3 1,-0.2 -2,-0.2 0.700 104.6 46.4 -71.1 -13.1 9.8 73.1 2.1 56 57 A L H X> S+ 0 0 0 -3,-2.4 3,-2.1 -4,-0.2 4,-1.2 0.745 88.6 83.7 -96.6 -28.9 6.8 75.1 1.0 57 58 A T H 3< S+ 0 0 2 -4,-0.8 3,-0.3 1,-0.3 -2,-0.1 0.797 86.6 52.0 -50.6 -43.0 7.0 75.0 -2.8 58 59 A T T 3< S+ 0 0 27 -4,-0.5 -1,-0.3 1,-0.2 -2,-0.1 0.566 106.0 58.1 -75.0 -3.6 5.3 71.7 -3.4 59 60 A V T <4 0 0 0 -3,-2.1 -1,-0.2 1,-0.1 -2,-0.2 0.800 360.0 360.0 -89.8 -32.0 2.4 72.9 -1.3 60 61 A F < 0 0 8 -4,-1.2 -2,-0.2 -3,-0.3 -3,-0.1 0.843 360.0 360.0 -76.8 360.0 1.9 75.9 -3.6 61 ! 0 0 0 0, 0.0 0, 0.0 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 360.0 0.0 0.0 0.0 62 65 A N > 0 0 7 0, 0.0 3,-1.8 0, 0.0 -3,-0.0 0.000 360.0 360.0 360.0 104.3 -2.6 71.3 -7.8 63 66 A R T 3 + 0 0 13 1,-0.3 3,-0.2 13,-0.0 13,-0.2 0.458 360.0 85.9 -71.1 -0.1 -2.4 68.2 -9.9 64 67 A V T 3 S+ 0 0 0 1,-0.3 2,-1.6 12,-0.2 -1,-0.3 0.814 79.8 70.9 -67.2 -26.1 -4.9 69.6 -12.3 65 68 A F S < S+ 0 0 1 -3,-1.8 2,-0.3 -30,-0.1 -1,-0.3 -0.529 78.6 101.8 -88.9 68.8 -1.7 71.1 -13.7 66 69 A A S S- 0 0 0 -2,-1.6 2,-0.9 -34,-0.6 -31,-0.3 -0.963 77.2-116.6-151.9 132.5 -0.4 67.8 -15.1 67 70 A K B -e 211 0A 81 143,-2.5 145,-2.7 -33,-0.3 -34,-0.1 -0.614 34.6-169.1 -76.4 105.1 -0.5 66.6 -18.7 68 71 A Y - 0 0 20 -2,-0.9 6,-0.1 143,-0.2 145,-0.1 -0.849 15.6-134.4 -99.2 122.6 -2.7 63.5 -18.7 69 72 A P > - 0 0 37 0, 0.0 3,-1.9 0, 0.0 144,-0.0 -0.312 28.8-106.8 -69.0 159.3 -2.7 61.4 -21.8 70 73 A E T 3 S+ 0 0 194 1,-0.3 4,-0.1 3,-0.0 -2,-0.0 0.762 115.8 63.7 -58.6 -25.4 -6.1 60.2 -23.1 71 74 A N T 3 S+ 0 0 110 2,-0.1 2,-0.4 1,-0.0 -1,-0.3 0.401 93.5 71.2 -83.0 3.9 -5.5 56.7 -21.9 72 75 A I S < S- 0 0 15 -3,-1.9 2,-0.2 111,-0.1 140,-0.1 -0.982 87.8-116.8-123.1 129.5 -5.3 57.6 -18.2 73 76 A V - 0 0 53 -2,-0.4 2,-1.0 109,-0.1 3,-0.1 -0.448 24.6-141.4 -64.1 126.0 -8.3 58.7 -16.1 74 77 A D > - 0 0 24 -2,-0.2 4,-1.3 1,-0.2 5,-0.1 -0.776 21.0-179.4 -95.0 96.8 -7.8 62.3 -15.0 75 78 A Y H > S+ 0 0 9 -2,-1.0 4,-0.9 1,-0.2 -1,-0.2 0.853 78.1 50.4 -61.7 -36.7 -9.2 62.4 -11.5 76 79 A F H > S+ 0 0 0 2,-0.2 4,-0.5 1,-0.2 3,-0.4 0.927 108.6 43.4 -73.6 -50.8 -8.4 66.1 -11.1 77 80 A K H >4 S+ 0 0 28 1,-0.2 3,-0.8 2,-0.2 -1,-0.2 0.839 112.0 58.9 -66.9 -28.5 -9.8 67.8 -14.2 78 81 A Q H 3< S+ 0 0 66 -4,-1.3 -1,-0.2 1,-0.2 -2,-0.2 0.830 93.7 66.9 -62.5 -38.4 -12.9 65.6 -13.6 79 82 A S H 3X S+ 0 0 0 -4,-0.9 4,-1.9 -3,-0.4 3,-0.3 0.713 87.9 129.9 -61.6 -24.4 -13.5 67.1 -10.2 80 83 A F T << + 0 0 7 -3,-0.8 -78,-0.1 -4,-0.5 -77,-0.1 -0.444 49.5 28.2 -85.4 162.9 -14.3 70.6 -11.4 81 84 A P T 4 S+ 0 0 65 0, 0.0 25,-0.5 0, 0.0 -1,-0.3 -0.980 132.3 42.8 -67.5 -19.1 -16.2 72.8 -11.3 82 85 A E T 4 S- 0 0 89 -3,-0.3 -2,-0.3 1,-0.2 24,-0.1 0.873 100.8-159.6 -57.5 -39.1 -16.8 71.6 -7.7 83 86 A G < - 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